RLG00000030858

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006300
Physical Location & Seq
Reverse (-)
235382 .. 243943
8562 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030858

Sequence Viewer

Length: 3498 bp
ATGAAGCTGGTGGTTCAAGTGATGGGGGCTCGAGATCTGCCGGCAATGGATCTAAACGGGCTGAGTGATCCGTACGTGAAGGTGAAGATGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACCTTAAACCCATATTGGGGTGAAGAGTTCACGTTTCGAGTCGACGACCTCAACGACGAGCTCCTCATCTCTTGTTTGGATGAAGATAAGTACTTCAACGACGACTTCGTGGGCTGCGTCAAATTCCCTGTTTCCCAAGTCTTCGATTCCCCCAACAAGTGCCTCGACACTTGTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAGGATTGCGGCGAGATTCTTCTTAATATCTCTCTTAGTACAAACAATGCATTCTCCGACTCCGCTTCCGCTTCCGCTTCCGCTTCCGCTTCCGATGGTGATCATATAAGGAGGGATTCCGACATTGGAGGTGAATCACCCTCAAGGTTATCCGAAACGGCTTCCCCTCACAGAGGCAAGCTTGATGAAGTTCCATTCTCCAAGGAAGATAAAGAAAAGGAAAAGACTCTTGCCCAGAAATCCCTTGCTGGCCGACTTGTTCAGATGTTCAATAAAAATCCTGACGCACCTCCAATATCTTCAAACCACTCTAGCAAAGCTGACTTGACTGAGCTTGTTGAAAACAAGGCCGACATTTCTGAGGCCACTTCTGAGGATCACTCCTCCTGTGTTCCTTTTGAAGAGCTAATGAAAACAATGCAGTTACGCGATCAGGCTACTGAAACCCCTGAGAACCTACCGGCTGGAGTGCTTCTAGATCAAATGTATGTTACTGAACCCAAACACCTCAACACTCTACTCTTTTCACCGGATTCCACTTTTCCAAAGGCGTTGGCAGATCTGCATGGAACTACAGAATTGGAACAAGGACCGTGGAAATTTGAAAATGATTGCTTAAAAAGAGTGGTTACATATGTTAAGGCAGCTAGCAAATTAATCAAGGCTTGCAAAGGAAGTGAGGACCAAACATATCTCAAAGCCGATGGGAAAGTTTTTGCAGTTTTATCAAGTGTAAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACCGAGTTGCTCTACTGCATTACACCTGGGCCTGAGCTGCCATCAGGAGAACAATCTACACGCTTTGTAATATCCTGGCGCATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGGCAAGGTTTAAAGGACAGTTATGAGCAGTATGCAACTTTATTATCGCAGAACGTTAAGCCAGCTGATTCTAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCATTGCAGGCTGAATCCCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTTATTGGATTGTACATGCTGGTACACATCTACCTGGCTACACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCTATTGGTGAATTCATTGTTTGTGGTGTCCTGGTACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCACGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGACCATGGAGTCAAAGCCCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAAGTGGGTTCTCTGATCCATACGTGGTGTTTTCTTGCAATGGAAAAACTAGAACTAGCTCAATCAAGTTCCAGAAATGCGACCCTATGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTTGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATATGTGGATTCCTCTTCAAGGAAAGTTAGCTCAGGCTTGTCAGTCAAAACTCCACTTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTTGTTAATCACTTTCTAAATAAGATGGAGAAGGAGGTTGGAAAGAAGATTACTGTGCGTTCTCCTCAAACAAATTCAGCATTCCAAAAACTCTTTGGGCTTCCACCAGAGGAATTTCTTATCAATGACTTTACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGGCGCCTATTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTCTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTCATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGTGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTCCAGTCCTTTGTATCCTTCAATGTAGCGAACAGGACAATTATGGCCCTGTGGAAGGCTAGATCTTTGAGTCCTGAGCAGAAGGTGCAAATAATAGAAGAAGAATCAGAAGTCAAGAGCCTCCAAACTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCATCCTTGCATTCAGTTCCTACAAATTTCTTCGCAGAGCTATTCGGTGGAGGTGAATTGGATCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTATTCTCACACCCCATGGGAATCAGAGAAGGGCGATGTCTATGTGAGGCAAATATATTACAGATATGACAAACGTGTTTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGCCTTTCTGATAGAAATGGTTGGCTTTTTCAAGAAGTCATGACCCTCCATGCAATTCCACTTGGTGACTATTTCAATGTTCACATCCGATACCAAATTGAGGACTCGTCCTCAACACCATCAGGGTGTCTGGTAAAAGTATACTTTGGCATTGAGTGGCTCAAAAGCACTAAGCATCAGAAAAGAATTACAAAAAATGTTCTCAAGAATCTGCAAGATCGCCTAAAGATGTGCAATAACTTGGGTCATCGGATTGTGAAGGTTAAAGCCTTGAGCCTTAAAGTAGATACAGAGGTGGCAGGTCTAGTTTTTGTGGTCTTGCCATTGCAGGCCAAACAACAGTTCTACTTCACTGTCAGTGGTGATCATTCTTTTAGAATAGCTCAAACTCGAAAGCGACTGGTGTCTTCTCCTTTGGCTTCAGTTTCATCTATTACTATCGAGGGTATCTGGTTTGTGCGCTGCATCTTTGATCCCTTTGATGGTGATGGGGTGAATGGCGTTAGTCTCTATTGGACGTGGTTTGCTGGTTTGATGGTGGTTATGGCGTTTGGCTGTGGACTAGCTGAAGGTAAGGCTGGCTTTCAACCTTCGTCTGGGGGCGGCGACGTCTCCTGTGATGGTGGTGTCGTGACAGTGCTTAGCGCCCGTTGGATCTGGCAAGCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1166

Amino Acids

130.2

Weight (kDa)

6.25

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 93 2.3e-29 C2 domain
VASt PF16016 271 - 417 2.7e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 552 - 653 2.8e-16 C2 domain
GRAM PF02893 705 - 812 3.9e-17 GRAM domain
VASt PF16016 877 - 1011 5.4e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1628
AatII GACGTC 1 cut(s) 3438
Acc36I ACCTGC 1 cut(s) 3116
AccB1I GGYRCC 2 cut(s) 303, 2202
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccI GTMKAC 2 cut(s) 168, 2967
AccII CGCG 1 cut(s) 768
AciI CCGC 9 cut(s) 348, 402, 408, 414, 420, 426, 2356, 2827, 3429
AclI AACGTT 1 cut(s) 1296
AclWI GGATC 9 cut(s) 57, 62, 723, 1320, 1333, 1712, 2679, 3293, 3488
AcoI YGGCCR 1 cut(s) 589
AcuI CTGAAG 4 cut(s) 2438, 2577, 3231, 3414
AcyI GRCGYC 2 cut(s) 2203, 3435
AdeI CACNNNGTG 1 cut(s) 2891
AfaI GTAC 8 cut(s) 74, 218, 379, 1392, 1442, 1452, 1554, 2812
AflIII ACRYGT 3 cut(s) 1567, 2168, 2779
AhlI ACTAGT 1 cut(s) 2807
AjiI CACGTC 1 cut(s) 3345
AjnI CCWGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
AjuI GAANNNNNNNTTGG 2 cut(s) 3152, 3184
Alw21I GWGCWC 3 cut(s) 189, 1211, 1243
Alw26I GTCTC 3 cut(s) 1423, 3338, 3442
AlwI GGATC 9 cut(s) 57, 62, 723, 1320, 1333, 1712, 2679, 3293, 3488
Ama87I CYCGRG 2 cut(s) 30, 1638
AoxI GGCC 9 cut(s) 589, 687, 702, 1139, 1599, 1894, 2472, 2578, 3156
ApeKI GCWGC 5 cut(s) 240, 983, 1147, 1691, 3288
AseI ATTAAT 1 cut(s) 995
Asp700I GAANNNNTTC 1 cut(s) 2295
AspLEI GCGC 4 cut(s) 1191, 2205, 3288, 3473
AspS9I GGNCC 9 cut(s) 929, 1021, 1110, 1139, 1336, 1868, 1894, 2473, 2578
AsuNHI GCTAGC 1 cut(s) 986
AvaI CYCGRG 2 cut(s) 30, 1638
AvaII GGWCC 5 cut(s) 929, 1021, 1110, 1336, 1868
BaeI ACNNNNGTAYC 2 cut(s) 3105, 3138
BamHI GGATCC 1 cut(s) 1325
BanI GGYRCC 2 cut(s) 303, 2202
BanII GRGCYC 3 cut(s) 31, 189, 1243
BbsI GAAGAC 3 cut(s) 131, 259, 3225
Bbv12I GWGCWC 3 cut(s) 189, 1211, 1243
BbvI GCAGC 5 cut(s) 227, 995, 1134, 1703, 3275
BceAI ACGGC 1 cut(s) 513
BciT130I CCWGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
BciVI GTATCC 1 cut(s) 2452
BclI TGATCA 2 cut(s) 439, 3190
BcoDI GTCTC 3 cut(s) 1423, 3338, 3442
BcuI ACTAGT 1 cut(s) 2807
BfaI CTAG 9 cut(s) 651, 815, 987, 1752, 1758, 2487, 2808, 3131, 3389
BfmI CTRYAG 5 cut(s) 912, 1202, 2195, 2340, 3494
BfoI RGCGCY 2 cut(s) 2206, 3474
BfuAI ACCTGC 1 cut(s) 3116
BfuI GTATCC 1 cut(s) 2452
BglII AGATCT 3 cut(s) 34, 898, 2489
BisI GCNGC 8 cut(s) 241, 349, 984, 1148, 1692, 2357, 3289, 3430
BlpI GCTNAGC 1 cut(s) 3467
BlsI GCNGC 8 cut(s) 242, 350, 985, 1149, 1693, 2358, 3290, 3431
BmcAI AGTACT 2 cut(s) 218, 2812
Bme1390I CCNGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
Bme18I GGWCC 5 cut(s) 929, 1021, 1110, 1336, 1868
BmeT110I CYCGRG 2 cut(s) 30, 1638
BmgBI CACGTC 1 cut(s) 3345
BmgT120I GGNCC 9 cut(s) 929, 1021, 1110, 1139, 1336, 1868, 1894, 2473, 2578
BmiI GGNNCC 4 cut(s) 305, 1327, 2204, 2234
BmrFI CCNGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
BmrI ACTGGG 1 cut(s) 1363
BmsI GCATC 5 cut(s) 1358, 1804, 2365, 3010, 3300
BmtI GCTAGC 1 cut(s) 990
BmuI ACTGGG 1 cut(s) 1363
BoxI GACNNNNGTC 1 cut(s) 3229
BpiI GAAGAC 3 cut(s) 131, 259, 3225
BplI GAGNNNNNCTC 2 cut(s) 704, 736
BpmI CTGGAG 2 cut(s) 296, 825
Bpu10I CCTNAGC 3 cut(s) 1143, 1974, 2502
Bpu1102I GCTNAGC 1 cut(s) 3467
BpuEI CTTGAG 4 cut(s) 466, 2020, 3014, 3118
BsaAI YACGTR 2 cut(s) 76, 1726
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 2 cut(s) 2203, 3435
BsaI GGTCTC 1 cut(s) 1423
BsaJI CCNNGG 7 cut(s) 540, 932, 1136, 1321, 1624, 1639, 2720
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 2 cut(s) 1554, 1584
Bse118I RCCGGY 2 cut(s) 40, 799
Bse1I ACTGG 3 cut(s) 1369, 2431, 3231
Bse3DI GCAATG 6 cut(s) 51, 1352, 1747, 1824, 2038, 3149
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
BseDI CCNNGG 7 cut(s) 540, 932, 1136, 1321, 1624, 1639, 2720
BseGI GGATG 7 cut(s) 211, 1828, 1849, 2380, 2407, 2612, 2910
BseJI GATNNNNATC 1 cut(s) 438
BseMI GCAATG 6 cut(s) 51, 1352, 1747, 1824, 2038, 3149
BseNI ACTGG 3 cut(s) 1369, 2431, 3231
BseRI GAGGAG 5 cut(s) 179, 712, 1656, 2088, 2811
BseXI GCAGC 5 cut(s) 227, 995, 1134, 1703, 3275
BseYI CCCAGC 2 cut(s) 1472, 1573
BsgI GTGCAG 1 cut(s) 1628
Bsh1236I CGCG 1 cut(s) 768
BshFI GGCC 9 cut(s) 591, 689, 704, 1141, 1601, 1896, 2474, 2580, 3158
BshNI GGYRCC 2 cut(s) 303, 2202
BsiHKAI GWGCWC 3 cut(s) 189, 1211, 1243
BsiHKCI CYCGRG 2 cut(s) 30, 1638
BsiSI CCGG 3 cut(s) 41, 800, 869
BsiWI CGTACG 1 cut(s) 72
BsmAI GTCTC 3 cut(s) 1423, 3338, 3442
BsmBI CGTCTC 1 cut(s) 3442
BsmI GAATGC 3 cut(s) 389, 2114, 2620
BsnI GGCC 9 cut(s) 591, 689, 704, 1141, 1601, 1896, 2474, 2580, 3158
Bso31I GGTCTC 1 cut(s) 1423
BsoBI CYCGRG 2 cut(s) 30, 1638
Bsp1286I GDGCHC 4 cut(s) 31, 189, 1211, 1243
Bsp1407I TGTACA 1 cut(s) 1440
Bsp1720I GCTNAGC 1 cut(s) 3467
Bsp19I CCATGG 2 cut(s) 1624, 2720
BspACI CCGC 9 cut(s) 348, 402, 408, 414, 420, 426, 2356, 2827, 3429
BspANI GGCC 9 cut(s) 591, 689, 704, 1141, 1601, 1896, 2474, 2580, 3158
BspFNI CGCG 1 cut(s) 768
BspHI TCATGA 2 cut(s) 2346, 2865
BspLI GGNNCC 4 cut(s) 305, 1327, 2204, 2234
BspMAI CTGCAG 2 cut(s) 1206, 2199
BspMI ACCTGC 1 cut(s) 3116
BspOI GCTAGC 1 cut(s) 990
BspPI GGATC 9 cut(s) 57, 62, 723, 1320, 1333, 1712, 2679, 3293, 3488
BspQI GCTCTTC 1 cut(s) 735
BspT107I GGYRCC 2 cut(s) 303, 2202
BspTNI GGTCTC 1 cut(s) 1423
BsrDI GCAATG 6 cut(s) 51, 1352, 1747, 1824, 2038, 3149
BsrFI RCCGGY 2 cut(s) 40, 799
BsrGI TGTACA 1 cut(s) 1440
BsrI ACTGG 3 cut(s) 1369, 2431, 3231
BssAI RCCGGY 2 cut(s) 40, 799
BssECI CCNNGG 7 cut(s) 540, 932, 1136, 1321, 1624, 1639, 2720
BssNAI GTATAC 1 cut(s) 2968
BssNI GRCGYC 2 cut(s) 2203, 3435
BssT1I CCWWGG 4 cut(s) 540, 1321, 1624, 2720
Bst1107I GTATAC 1 cut(s) 2968
Bst2UI CCWGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
Bst4CI ACNGT 8 cut(s) 933, 1262, 1414, 1663, 2089, 3168, 3181, 3463
Bst6I CTCTTC 4 cut(s) 144, 735, 1962, 2553
BstACI GRCGYC 2 cut(s) 2203, 3435
BstAPI GCANNNNNTGC 1 cut(s) 2512
BstAUI TGTACA 1 cut(s) 1440
BstBAI YACGTR 2 cut(s) 76, 1726
BstDSI CCRYGG 3 cut(s) 932, 1624, 2720
BstENI CCTNNNNNAGG 4 cut(s) 510, 1959, 2279, 2480
BstF5I GGATG 7 cut(s) 211, 1828, 1849, 2380, 2407, 2612, 2910
BstFNI CGCG 1 cut(s) 768
BstH2I RGCGCY 2 cut(s) 2206, 3474
BstHHI GCGC 4 cut(s) 1191, 2205, 3288, 3473
BstMAI GTCTC 3 cut(s) 1423, 3338, 3442
BstMWI GCNNNNNNNGC 3 cut(s) 1147, 1510, 2512
BstNI CCWGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
BstNSI RCATGY 3 cut(s) 1447, 2172, 2599
BstPAI GACNNNNGTC 1 cut(s) 3229
BstSCI CCNGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
BstSFI CTRYAG 5 cut(s) 912, 1202, 2195, 2340, 3494
BstUI CGCG 1 cut(s) 768
BstV1I GCAGC 5 cut(s) 227, 995, 1134, 1703, 3275
BstV2I GAAGAC 3 cut(s) 131, 259, 3225
BstX2I RGATCY 6 cut(s) 34, 49, 898, 1325, 2489, 3480
BstYI RGATCY 6 cut(s) 34, 49, 898, 1325, 2489, 3480
BstZ17I GTATAC 1 cut(s) 2968
BsuI GTATCC 1 cut(s) 2452
BsuRI GGCC 9 cut(s) 591, 689, 704, 1141, 1601, 1896, 2474, 2580, 3158
BtgI CCRYGG 3 cut(s) 932, 1624, 2720
BtgZI GCGATG 1 cut(s) 2754
BtrI CACGTC 1 cut(s) 3345
BtsCI GGATG 7 cut(s) 211, 1828, 1849, 2380, 2407, 2612, 2910
BtsI GCAGTG 1 cut(s) 1624
BtsIMutI CAGTG 5 cut(s) 1410, 1624, 3177, 3190, 3468
BveI ACCTGC 1 cut(s) 3116
CciI TCATGA 2 cut(s) 2346, 2865
CfoI GCGC 4 cut(s) 1191, 2205, 3288, 3473
Cfr10I RCCGGY 2 cut(s) 40, 799
Cfr13I GGNCC 9 cut(s) 929, 1021, 1110, 1139, 1336, 1868, 1894, 2473, 2578
CseI GACGC 2 cut(s) 232, 632
CsiI ACCWGGT 1 cut(s) 1338
Csp6I GTAC 8 cut(s) 73, 217, 378, 1391, 1441, 1451, 1553, 2811
CspCI CAANNNNNGTGG 2 cut(s) 914, 949
CviQI GTAC 8 cut(s) 73, 217, 378, 1391, 1441, 1451, 1553, 2811
DinI GGCGCC 1 cut(s) 2204
DraI TTTAAA 1 cut(s) 1254
DraIII CACNNNGTG 1 cut(s) 2891
DrdI GACNNNNNNGTC 1 cut(s) 1628
DseDI GACNNNNNNGTC 1 cut(s) 1628
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 4 cut(s) 144, 735, 1962, 2553
EarI CTCTTC 4 cut(s) 144, 735, 1962, 2553
Ecl136II GAGCTC 2 cut(s) 187, 1241
Eco130I CCWWGG 4 cut(s) 540, 1321, 1624, 2720
Eco24I GRGCYC 3 cut(s) 31, 189, 1243
Eco31I GGTCTC 1 cut(s) 1423
Eco47I GGWCC 5 cut(s) 929, 1021, 1110, 1336, 1868
Eco53kI GAGCTC 2 cut(s) 187, 1241
Eco57I CTGAAG 4 cut(s) 2438, 2577, 3231, 3414
Eco88I CYCGRG 2 cut(s) 30, 1638
EcoICRI GAGCTC 2 cut(s) 187, 1241
EcoNI CCTNNNNNAGG 4 cut(s) 510, 1959, 2279, 2480
EcoRI GAATTC 1 cut(s) 1529
EcoRII CCWGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
EcoT14I CCWWGG 4 cut(s) 540, 1321, 1624, 2720
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 3 cut(s) 31, 189, 1243
EgeI GGCGCC 1 cut(s) 2204
EheI GGCGCC 1 cut(s) 2204
ErhI CCWWGG 4 cut(s) 540, 1321, 1624, 2720
Esp3I CGTCTC 1 cut(s) 3442
FalI AAGNNNNNCTT 6 cut(s) 113, 145, 2411, 2443, 3392, 3424
FauI CCCGC 1 cut(s) 2834
FauNDI CATATG 2 cut(s) 973, 1093
FbaI TGATCA 2 cut(s) 439, 3190
FblI GTMKAC 2 cut(s) 168, 2967
Fnu4HI GCNGC 8 cut(s) 241, 349, 984, 1148, 1692, 2357, 3289, 3430
FokI GGATG 7 cut(s) 218, 1835, 1856, 2387, 2414, 2599, 2897
FriOI GRGCYC 3 cut(s) 31, 189, 1243
Fsp4HI GCNGC 8 cut(s) 241, 349, 984, 1148, 1692, 2357, 3289, 3430
FspBI CTAG 9 cut(s) 651, 815, 987, 1752, 1758, 2487, 2808, 3131, 3389
GlaI GCGC 4 cut(s) 1190, 2204, 3287, 3472
GluI GCNGC 8 cut(s) 241, 349, 984, 1148, 1692, 2357, 3289, 3430
GsaI CCCAGC 2 cut(s) 1476, 1577
GsuI CTGGAG 2 cut(s) 296, 825
HaeII RGCGCY 2 cut(s) 2206, 3474
HaeIII GGCC 9 cut(s) 591, 689, 704, 1141, 1601, 1896, 2474, 2580, 3158
HapII CCGG 3 cut(s) 41, 800, 869
HgaI GACGC 2 cut(s) 232, 632
HhaI GCGC 4 cut(s) 1191, 2205, 3288, 3473
Hin1I GRCGYC 2 cut(s) 2203, 3435
Hin6I GCGC 4 cut(s) 1189, 2203, 3286, 3471
HinP1I GCGC 4 cut(s) 1189, 2203, 3286, 3471
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 518
HpaII CCGG 3 cut(s) 41, 800, 869
Hpy166II GTNNAC 6 cut(s) 156, 169, 1453, 2908, 2968, 3386
Hpy8I GTNNAC 6 cut(s) 156, 169, 1453, 2908, 2968, 3386
Hpy99I CGWCG 4 cut(s) 173, 185, 230, 3437
HpyCH4III ACNGT 8 cut(s) 933, 1262, 1414, 1663, 2089, 3168, 3181, 3463
HpyCH4IV ACGT 8 cut(s) 75, 158, 1296, 1567, 1725, 2779, 3344, 3435
HpyF10VI GCNNNNNNNGC 3 cut(s) 1147, 1510, 2512
HpySE526I ACGT 8 cut(s) 75, 158, 1296, 1567, 1725, 2779, 3344, 3435
Hsp92I GRCGYC 2 cut(s) 2203, 3435
HspAI GCGC 4 cut(s) 1189, 2203, 3286, 3471
KasI GGCGCC 1 cut(s) 2202
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 2 cut(s) 439, 3190
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 2 cut(s) 192, 1238
Lsp1109I GCAGC 5 cut(s) 227, 995, 1134, 1703, 3275
LweI GCATC 5 cut(s) 1358, 1804, 2365, 3010, 3300
MabI ACCWGGT 1 cut(s) 1338
MaeI CTAG 9 cut(s) 651, 815, 987, 1752, 1758, 2487, 2808, 3131, 3389
MaeII ACGT 8 cut(s) 75, 158, 1296, 1567, 1725, 2779, 3344, 3435
MaeIII GTNAC 8 cut(s) 762, 829, 967, 1619, 2171, 2803, 2891, 3457
MflI RGATCY 6 cut(s) 34, 49, 898, 1325, 2489, 3480
MhlI GDGCHC 4 cut(s) 31, 189, 1211, 1243
Mly113I GGCGCC 1 cut(s) 2203
MlyI GAGTC 7 cut(s) 174, 392, 559, 1638, 2344, 2506, 2924
MmeI TCCRAC 7 cut(s) 420, 483, 1470, 2053, 2336, 2360, 3458
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 2295
MslI CAYNNNNRTG 2 cut(s) 1214, 2950
MspA1I CMGCKG 2 cut(s) 1307, 1694
MspI CCGG 3 cut(s) 41, 800, 869
MspR9I CCNGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
Mva1269I GAATGC 3 cut(s) 389, 2114, 2620
MvaI CCWGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
MvnI CGCG 1 cut(s) 768
MwoI GCNNNNNNNGC 3 cut(s) 1147, 1510, 2512
NaeI GCCGGC 1 cut(s) 42
NarI GGCGCC 1 cut(s) 2203
NcoI CCATGG 2 cut(s) 1624, 2720
NdeI CATATG 2 cut(s) 973, 1093
NgoMIV GCCGGC 1 cut(s) 40
NheI GCTAGC 1 cut(s) 986
NlaIV GGNNCC 4 cut(s) 305, 1327, 2204, 2234
NmeAIII GCCGAG 1 cut(s) 1222
NmuCI GTSAC 5 cut(s) 1619, 2171, 2803, 2891, 3457
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 3 cut(s) 1447, 2172, 2599
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 2 cut(s) 2346, 2865
PciI ACATGT 1 cut(s) 2168
PciSI GCTCTTC 1 cut(s) 735
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 3 cut(s) 389, 2114, 2620
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 2295
Pfl23II CGTACG 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 1345
PkrI GCNGC 8 cut(s) 242, 350, 985, 1149, 1693, 2358, 3290, 3431
PleI GAGTC 7 cut(s) 173, 392, 559, 1637, 2344, 2505, 2924
PluTI GGCGCC 1 cut(s) 2206
PpsI GAGTC 7 cut(s) 173, 392, 559, 1637, 2344, 2505, 2924
Ppu21I YACGTR 2 cut(s) 76, 1726
PscI ACATGT 1 cut(s) 2168
PshAI GACNNNNGTC 1 cut(s) 3229
PshBI ATTAAT 1 cut(s) 995
Psp124BI GAGCTC 2 cut(s) 189, 1243
Psp1406I AACGTT 1 cut(s) 1296
Psp6I CCWGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
PspFI CCCAGC 2 cut(s) 1472, 1573
PspGI CCWGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 305, 1327, 2204, 2234
PspPI GGNCC 9 cut(s) 929, 1021, 1110, 1139, 1336, 1868, 1894, 2473, 2578
PstI CTGCAG 2 cut(s) 1206, 2199
PsuI RGATCY 6 cut(s) 34, 49, 898, 1325, 2489, 3480
PvuII CAGCTG 2 cut(s) 1307, 1694
RsaI GTAC 8 cut(s) 74, 218, 379, 1392, 1442, 1452, 1554, 2812
RsaNI GTAC 8 cut(s) 73, 217, 378, 1391, 1441, 1451, 1553, 2811
RseI CAYNNNNRTG 2 cut(s) 1214, 2950
SacI GAGCTC 2 cut(s) 189, 1243
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 735
SatI GCNGC 8 cut(s) 241, 349, 984, 1148, 1692, 2357, 3289, 3430
Sau96I GGNCC 9 cut(s) 929, 1021, 1110, 1139, 1336, 1868, 1894, 2473, 2578
SbfI CCTGCAGG 1 cut(s) 2199
ScaI AGTACT 2 cut(s) 218, 2812
SchI GAGTC 7 cut(s) 174, 392, 559, 1638, 2344, 2506, 2924
ScrFI CCNGG 5 cut(s) 1137, 1186, 1340, 1463, 1550
SdaI CCTGCAGG 1 cut(s) 2199
SduI GDGCHC 4 cut(s) 31, 189, 1211, 1243
SexAI ACCWGGT 1 cut(s) 1338
SfaNI GCATC 5 cut(s) 1358, 1804, 2365, 3010, 3300
SfcI CTRYAG 5 cut(s) 912, 1202, 2195, 2340, 3494
SfoI GGCGCC 1 cut(s) 2204
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 5 cut(s) 929, 1021, 1110, 1336, 1868
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 1214, 2950
SmlI CTYRAG 5 cut(s) 30, 481, 1999, 3029, 3097
SmoI CTYRAG 5 cut(s) 30, 481, 1999, 3029, 3097
SpeI ACTAGT 1 cut(s) 2807
Sse8387I CCTGCAGG 1 cut(s) 2199
SsiI CCGC 9 cut(s) 348, 402, 408, 414, 420, 426, 2356, 2827, 3429
SspDI GGCGCC 1 cut(s) 2202
SspI AATATT 2 cut(s) 1397, 1803
SspMI CTAG 9 cut(s) 651, 815, 987, 1752, 1758, 2487, 2808, 3131, 3389
SstI GAGCTC 2 cut(s) 189, 1243
StyD4I CCNGG 5 cut(s) 1135, 1184, 1338, 1461, 1548
StyI CCWWGG 4 cut(s) 540, 1321, 1624, 2720
TaaI ACNGT 8 cut(s) 933, 1262, 1414, 1663, 2089, 3168, 3181, 3463
TaiI ACGT 8 cut(s) 78, 161, 1299, 1570, 1728, 2782, 3347, 3438
TaqI TCGA 7 cut(s) 31, 163, 168, 270, 291, 3217, 3267
TaqII GACCGA 1 cut(s) 1127
TatI WGTACW 5 cut(s) 216, 377, 1390, 1440, 2810
TauI GCSGC 3 cut(s) 351, 2359, 3432
TscAI CASTG 5 cut(s) 1417, 1624, 3184, 3190, 3468
TseFI GTSAC 5 cut(s) 1619, 2171, 2803, 2891, 3457
TseI GCWGC 5 cut(s) 240, 983, 1147, 1691, 3288
Tsp45I GTSAC 5 cut(s) 1619, 2171, 2803, 2891, 3457
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 5 cut(s) 1417, 1624, 3184, 3190, 3468
Van91I CCANNNNNTGG 1 cut(s) 1345
VpaK11BI GGWCC 5 cut(s) 929, 1021, 1110, 1336, 1868
VspI ATTAAT 1 cut(s) 995
XagI CCTNNNNNAGG 4 cut(s) 510, 1959, 2279, 2480
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 3 cut(s) 1447, 2172, 2599
XcmI CCANNNNNNNNNTGG 1 cut(s) 2126
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 168, 2967
XmnI GAANNNNTTC 1 cut(s) 2295
XspI CTAG 9 cut(s) 651, 815, 987, 1752, 1758, 2487, 2808, 3131, 3389
ZraI GACGTC 1 cut(s) 3436
ZrmI AGTACT 2 cut(s) 218, 2812
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.