Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0006300

Physical Location & Seq

Genomic Coordinates Reverse (-)

199993 .. 205962

Gene Structure 5970 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG002300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2727 bp

ATGAAGCTGGTGGTTCAAGTGATGGGGGCTCGAGATCTGCCGGCAATGGATCTAAACGGGCTGAGTGATCCGTACGTGAAGGTGAAGATGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACGTTAAACCCATATTGGGGTGAAGAGTTCACGTTTCGAGTCGACGACCTCAACGACGAGCTCCTCATCTCTTGTTTGGATGAAGATAAGTACTTCAACGACGACTTCGTGGGCTGCGTCAAATTCCCTGTTTCCCAAGTCTTCGATTCCCCCAACAAGTGCCTCGACACTTGTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAGGATTGCGGCGAGATTCTTCTTAATATCTCTCTCAGTACAAACAATGCATTCTCCGACTCCGCTTCCGATGGTGATCACATCAGGAGGGATTCCGACATTGGAGGTGAATCACCCTCAAGGTTATCCGAAACGGCTTCCCCTCACAGAGGCAGGCTTGATGAACTTCCATTCTCCAAGGAAGATAAAGAAAAGGAAAAGAGTACTCTTGCCCAGAAATCCCTTGCTGGCCGACTTGTTCAGATGTTCAATAAAAATCCTGACGCACCTCCAATATCTTCAAACCACTCTAGCAAAGCTGACTTGACTGAGCTTGTTGACATTTCTGAGGCCACTTATTCTGAGGATCACTCCTCTTGTGTTCCTTTTGAAGAGCTAATGAAAACAATGCAGTTACGCGATCAGGCTACTGAAACCCCCGATAACCTACCCGGTGGAGTGCTTCTAGATCAAATGTATGTTACTGAACCCAAACACCTCAACACTCTACTCTTTTCTCCGGATTCTACTTTTCCAAAGGCGTTGGCAGATCTGCATGGAACTACAGAATTGGAACAAGGACCCTGGAAATTTGAAAACGACAGCTTAAAAAGAGTGGTTACATATGTTAAGGCAGCTAGCAAATTAATCAAGGCTTGCAAAGGAAGTGAGGACCAAACATATCTCAAAGCCGATGGGAAAGTTTTTGCAGTTTTATCAAGTGTGAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACGGAGTTGCTCTACTGCATTACACCTGGGCCTGAGCTGCCATCGGGAGAACAATCTACACGCTTTGTAATATCCTGGCGCATGAATTTTCTTCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGGCAAGGTTTAAAGGACAGTTATGAGCAGTATGCAACTTTATTATCGCAGAACGTTAAGCCAGCTGATTCTAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCATTGCAGGCTGAATCCCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTTATTGGATTGTATATGCTGGTACACATCTACCTGGCTACACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCAATTGGTGAATTCATTGTTTGTGGTGTCCTGGTACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCACGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGACCATGGAGTCAAAGCCCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAAGTGGGTTCTCTGATCCATACGTGGTGTTTTCTTGCAATGGAAAAGCTAGAACTAGCTCAATCAAGTTCCAGAAATGCGACCCTATGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTTGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATATGTGGATTCCTCTTCAAGGAAAGTTAGCTCAGGCTTGTCAGTCAAAACTCCACTTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTTGTTAATCACTTTTTAAATAAGATGGAGAAGGAGGTTGGAAAGAAGATTACTGTACGTTCTCCTCAAACAAATTCAGCATTCCAAAAACTCTTTGGGCTTCCACCAGAGGAATTTCTTATCAACGACTTTACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTCTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTCATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGGGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTCCAGTCCTTCGTATCCTTCAATGTAGCGAACAGGACAATTATGGCCCTGTGGAAGGCTAGATCTTTGAGTCCTGAGCAGAAGGTGCAAATAATAGAAGAAGAATCAGAAGTCAAGAGCCTCCAAACTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCATCCTTGCATTCAGTTCCTGTGAGCACTTTCTCTCTGGACCATACATGTACCCTTTGTTGTTTTTCTTTCCTTCCCTTTTCGGTACAAATATTCACATGTGTTTTTTGGACCTGCAGTAAAGCTGCATCTCCTTTAGAATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 DUF4782 GRAM

Protein Analysis

908

Amino Acids

101.46

Weight (kDa)

5.62

Isoelectric Point (pI)

49.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	2 - 93	1.7e-29	C2 domain
VASt	PF16016	261 - 407	2.8e-32	VAD1 Analog of StAR-related lipid transfer domain
C2	PF00168	542 - 643	3.4e-16	C2 domain
GRAM	PF02893	695 - 802	2.8e-17	GRAM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006300)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03370 AT1G03370
fragaria_vesca	FvH4_3g00230 FvH4_3g00230 FvH4_3g00230 FvH4_3g00230
malus_domestica	MD05G1362700.v1.1 MD10G1340200.v1.1
prunus_persica	Prupe.4G002600_v2.0.a1 Prupe.4G002600_v2.0.a1 Prupe.4G002600_v2.0.a1 Prupe.4G002600_v2.0.a1 Prupe.4G002600_v2.0.a1 Prupe.4G002600_v2.0.a1
pyrus_communis	pycom05g32910 pycom10g29010
rosa_chinensis	RchiOBHm_Chr5g0000311
rosa_laevigata	RLG00000030858
rosa_multiflora	Rmu_ssc0000385.1_g000007
rosa_roxburghii	Rroxscaffold_1G00075810
rosa_rugosa	Rorug04G0383000 Rorug04G0383100 Rorug04G0383100
rosa_samantha	Rh5AG002300 Rh5CG002600 Rh5DG002600
rosa_wichuraiana	Rw5G000280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1598
Acc36I	ACCTGC	1 cut(s)	2702
AccB1I	GGYRCC	1 cut(s)	303
AccB7I	CCANNNNNTGG	1 cut(s)	1315
AccI	GTMKAC	1 cut(s)	168
AccII	CGCG	1 cut(s)	738
AccIII	TCCGGA	1 cut(s)	838
AciI	CCGC	4 cut(s)	348, 402, 2173, 2326
AclI	AACGTT	1 cut(s)	1266
AclWI	GGATC	6 cut(s)	57, 62, 693, 1290, 1303, 1682
AcoI	YGGCCR	1 cut(s)	568
AcsI	RAATTY	9 cut(s)	248, 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212
AcuI	CTGAAG	3 cut(s)	1157, 2408, 2547
AfiI	CCNNNNNNNGG	8 cut(s)	142, 143, 488, 1315, 1931, 2168, 2251, 2452
AflIII	ACRYGT	4 cut(s)	1537, 2138, 2627, 2678
AjnI	CCWGG	6 cut(s)	902, 1105, 1154, 1308, 1431, 1518
Alw21I	GWGCWC	5 cut(s)	189, 1049, 1181, 1213, 2609
Alw26I	GTCTC	1 cut(s)	1393
AlwI	GGATC	6 cut(s)	57, 62, 693, 1290, 1303, 1682
AlwNI	CAGNNNCTG	1 cut(s)	2600
Ama87I	CYCGRG	2 cut(s)	30, 1608
Aor13HI	TCCGGA	1 cut(s)	838
AoxI	GGCC	8 cut(s)	568, 669, 1109, 1569, 1864, 2170, 2442, 2548
ApeKI	GCWGC	5 cut(s)	240, 953, 1117, 1661, 2705
ApoI	RAATTY	9 cut(s)	248, 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212
AseI	ATTAAT	1 cut(s)	965
Asp700I	GAANNNNTTC	1 cut(s)	2265
AspLEI	GCGC	1 cut(s)	1161
AsuC2I	CCSGG	1 cut(s)	771
AsuHPI	GGTGA	7 cut(s)	94, 130, 158, 425, 444, 458, 1508
AsuNHI	GCTAGC	1 cut(s)	956
AvaI	CYCGRG	2 cut(s)	30, 1608
AvaII	GGWCC	6 cut(s)	899, 991, 1306, 1838, 2620, 2691
BamHI	GGATCC	1 cut(s)	1295
BanI	GGYRCC	1 cut(s)	303
BanII	GRGCYC	3 cut(s)	31, 189, 1213
BbsI	GAAGAC	2 cut(s)	131, 259
Bbv12I	GWGCWC	5 cut(s)	189, 1049, 1181, 1213, 2609
BbvI	GCAGC	5 cut(s)	227, 965, 1104, 1673, 2692
BccI	CCATC	8 cut(s)	16, 82, 404, 1007, 1129, 1610, 1829, 2023
BceAI	ACGGC	1 cut(s)	489
BciT130I	CCWGG	6 cut(s)	904, 1107, 1156, 1310, 1433, 1520
BciVI	GTATCC	1 cut(s)	2422
BclI	TGATCA	1 cut(s)	415
BcnI	CCSGG	1 cut(s)	771
BcoDI	GTCTC	1 cut(s)	1393
BfaI	CTAG	6 cut(s)	630, 785, 957, 1722, 1728, 2457
BfmI	CTRYAG	4 cut(s)	882, 2165, 2310, 2695
BfuAI	ACCTGC	1 cut(s)	2702
BfuI	GTATCC	1 cut(s)	2422
BglII	AGATCT	3 cut(s)	34, 868, 2459
BisI	GCNGC	8 cut(s)	241, 349, 954, 1118, 1662, 2173, 2327, 2706
BlsI	GCNGC	8 cut(s)	242, 350, 955, 1119, 1663, 2174, 2328, 2707
BmcAI	AGTACT	2 cut(s)	218, 544
Bme1390I	CCNGG	7 cut(s)	771, 904, 1107, 1156, 1310, 1433, 1520
Bme18I	GGWCC	6 cut(s)	899, 991, 1306, 1838, 2620, 2691
BmeT110I	CYCGRG	2 cut(s)	30, 1608
BmiI	GGNNCC	4 cut(s)	305, 901, 1297, 2204
BmrFI	CCNGG	7 cut(s)	771, 904, 1107, 1156, 1310, 1433, 1520
BmrI	ACTGGG	1 cut(s)	1333
BmsI	GCATC	4 cut(s)	1328, 1774, 2335, 2717
BmtI	GCTAGC	1 cut(s)	960
BmuI	ACTGGG	1 cut(s)	1333
BpiI	GAAGAC	2 cut(s)	131, 259
BplI	GAGNNNNNCTC	2 cut(s)	674, 706
BpmI	CTGGAG	1 cut(s)	296
Bpu10I	CCTNAGC	3 cut(s)	1113, 1944, 2472
BpuEI	CTTGAG	2 cut(s)	442, 1990
BpuMI	CCSGG	1 cut(s)	771
BsaAI	YACGTR	2 cut(s)	76, 1696
BsaBI	GATNNNNATC	1 cut(s)	414
BsaI	GGTCTC	1 cut(s)	1393
BsaJI	CCNNGG	6 cut(s)	516, 902, 1106, 1291, 1594, 1609
BsaWI	WCCGGW	1 cut(s)	838
BsaXI	ACNNNNNCTCC	2 cut(s)	1524, 1554
Bsc4I	CCNNNNNNNGG	8 cut(s)	142, 143, 488, 1315, 1931, 2168, 2251, 2452
Bse118I	RCCGGY	1 cut(s)	40
Bse1I	ACTGG	2 cut(s)	1339, 2401
Bse3DI	GCAATG	5 cut(s)	51, 1322, 1717, 1794, 2008
Bse8I	GATNNNNATC	1 cut(s)	414
BseAI	TCCGGA	1 cut(s)	838
BseBI	CCWGG	6 cut(s)	904, 1107, 1156, 1310, 1433, 1520
BseDI	CCNNGG	6 cut(s)	516, 902, 1106, 1291, 1594, 1609
BseGI	GGATG	6 cut(s)	211, 1798, 1819, 2350, 2377, 2582
BseJI	GATNNNNATC	1 cut(s)	414
BseLI	CCNNNNNNNGG	8 cut(s)	142, 143, 488, 1315, 1931, 2168, 2251, 2452
BseMI	GCAATG	5 cut(s)	51, 1322, 1717, 1794, 2008
BseMII	CTCAG	9 cut(s)	53, 388, 639, 657, 672, 1104, 1958, 2463, 2562
BseNI	ACTGG	2 cut(s)	1339, 2401
BseRI	GAGGAG	4 cut(s)	179, 682, 1626, 2058
BseXI	GCAGC	5 cut(s)	227, 965, 1104, 1673, 2692
BseYI	CCCAGC	2 cut(s)	1442, 1543
BsgI	GTGCAG	1 cut(s)	1598
Bsh1236I	CGCG	1 cut(s)	738
BshFI	GGCC	8 cut(s)	570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BshNI	GGYRCC	1 cut(s)	303
BsiHKAI	GWGCWC	5 cut(s)	189, 1049, 1181, 1213, 2609
BsiHKCI	CYCGRG	2 cut(s)	30, 1608
BsiSI	CCGG	3 cut(s)	41, 771, 839
BsiWI	CGTACG	1 cut(s)	72
BslI	CCNNNNNNNGG	8 cut(s)	142, 143, 488, 1315, 1931, 2168, 2251, 2452
BsmAI	GTCTC	1 cut(s)	1393
BsmI	GAATGC	3 cut(s)	389, 2084, 2590
BsnI	GGCC	8 cut(s)	570, 671, 1111, 1571, 1866, 2172, 2444, 2550
Bso31I	GGTCTC	1 cut(s)	1393
BsoBI	CYCGRG	2 cut(s)	30, 1608
Bsp1286I	GDGCHC	6 cut(s)	31, 189, 1049, 1181, 1213, 2609
Bsp13I	TCCGGA	1 cut(s)	838
Bsp19I	CCATGG	1 cut(s)	1594
BspACI	CCGC	4 cut(s)	348, 402, 2173, 2326
BspANI	GGCC	8 cut(s)	570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BspCNI	CTCAG	9 cut(s)	54, 387, 640, 658, 673, 1105, 1957, 2464, 2563
BspEI	TCCGGA	1 cut(s)	838
BspFNI	CGCG	1 cut(s)	738
BspHI	TCATGA	1 cut(s)	2316
BspLI	GGNNCC	4 cut(s)	305, 901, 1297, 2204
BspMAI	CTGCAG	2 cut(s)	2169, 2699
BspMI	ACCTGC	1 cut(s)	2702
BspOI	GCTAGC	1 cut(s)	960
BspPI	GGATC	6 cut(s)	57, 62, 693, 1290, 1303, 1682
BspQI	GCTCTTC	1 cut(s)	705
BspT107I	GGYRCC	1 cut(s)	303
BspTNI	GGTCTC	1 cut(s)	1393
BsrDI	GCAATG	5 cut(s)	51, 1322, 1717, 1794, 2008
BsrFI	RCCGGY	1 cut(s)	40
BsrI	ACTGG	2 cut(s)	1339, 2401
BssAI	RCCGGY	1 cut(s)	40
BssECI	CCNNGG	6 cut(s)	516, 902, 1106, 1291, 1594, 1609
BssT1I	CCWWGG	3 cut(s)	516, 1291, 1594
Bst2UI	CCWGG	6 cut(s)	904, 1107, 1156, 1310, 1433, 1520
Bst4CI	ACNGT	4 cut(s)	1232, 1384, 1633, 2059
Bst6I	CTCTTC	4 cut(s)	144, 705, 1932, 2523
BstAPI	GCANNNNNTGC	1 cut(s)	2482
BstBAI	YACGTR	2 cut(s)	76, 1696
BstDEI	CTNAG	9 cut(s)	62, 374, 648, 666, 681, 1113, 1944, 2472, 2571
BstDSI	CCRYGG	1 cut(s)	1594
BstENI	CCTNNNNNAGG	4 cut(s)	486, 1929, 2249, 2450
BstF5I	GGATG	6 cut(s)	211, 1798, 1819, 2350, 2377, 2582
BstFNI	CGCG	1 cut(s)	738
BstHHI	GCGC	1 cut(s)	1161
BstMAI	GTCTC	1 cut(s)	1393
BstMWI	GCNNNNNNNGC	3 cut(s)	1117, 1480, 2482
BstNI	CCWGG	6 cut(s)	904, 1107, 1156, 1310, 1433, 1520
BstNSI	RCATGY	4 cut(s)	2142, 2569, 2631, 2682
BstSCI	CCNGG	7 cut(s)	769, 902, 1105, 1154, 1308, 1431, 1518
BstSFI	CTRYAG	4 cut(s)	882, 2165, 2310, 2695
BstUI	CGCG	1 cut(s)	738
BstV1I	GCAGC	5 cut(s)	227, 965, 1104, 1673, 2692
BstV2I	GAAGAC	2 cut(s)	131, 259
BstX2I	RGATCY	5 cut(s)	34, 49, 868, 1295, 2459
BstYI	RGATCY	5 cut(s)	34, 49, 868, 1295, 2459
BsuI	GTATCC	1 cut(s)	2422
BsuRI	GGCC	8 cut(s)	570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BtgI	CCRYGG	1 cut(s)	1594
BtsCI	GGATG	6 cut(s)	211, 1798, 1819, 2350, 2377, 2582
BtsI	GCAGTG	1 cut(s)	1594
BtsIMutI	CAGTG	2 cut(s)	1380, 1594
BveI	ACCTGC	1 cut(s)	2702
CaiI	CAGNNNCTG	1 cut(s)	2600
CciI	TCATGA	1 cut(s)	2316
CfoI	GCGC	1 cut(s)	1161
Cfr10I	RCCGGY	1 cut(s)	40
CseI	GACGC	2 cut(s)	232, 611
CsiI	ACCWGGT	1 cut(s)	1308
DdeI	CTNAG	9 cut(s)	62, 374, 648, 666, 681, 1113, 1944, 2472, 2571
DraI	TTTAAA	2 cut(s)	1224, 2022
DrdI	GACNNNNNNGTC	1 cut(s)	1598
DseDI	GACNNNNNNGTC	1 cut(s)	1598
EaeI	YGGCCR	1 cut(s)	568
Eam1104I	CTCTTC	4 cut(s)	144, 705, 1932, 2523
EarI	CTCTTC	4 cut(s)	144, 705, 1932, 2523
Ecl136II	GAGCTC	2 cut(s)	187, 1211
Eco130I	CCWWGG	3 cut(s)	516, 1291, 1594
Eco24I	GRGCYC	3 cut(s)	31, 189, 1213
Eco31I	GGTCTC	1 cut(s)	1393
Eco47I	GGWCC	6 cut(s)	899, 991, 1306, 1838, 2620, 2691
Eco53kI	GAGCTC	2 cut(s)	187, 1211
Eco57I	CTGAAG	3 cut(s)	1157, 2408, 2547
Eco88I	CYCGRG	2 cut(s)	30, 1608
EcoICRI	GAGCTC	2 cut(s)	187, 1211
EcoNI	CCTNNNNNAGG	4 cut(s)	486, 1929, 2249, 2450
EcoO109I	RGGNCCY	1 cut(s)	899
EcoRI	GAATTC	1 cut(s)	1499
EcoRII	CCWGG	6 cut(s)	902, 1105, 1154, 1308, 1431, 1518
EcoT14I	CCWWGG	3 cut(s)	516, 1291, 1594
EcoT22I	ATGCAT	1 cut(s)	391
EcoT38I	GRGCYC	3 cut(s)	31, 189, 1213
ErhI	CCWWGG	3 cut(s)	516, 1291, 1594
FalI	AAGNNNNNCTT	2 cut(s)	2381, 2413
FauNDI	CATATG	2 cut(s)	943, 1063
FbaI	TGATCA	1 cut(s)	415
FblI	GTMKAC	1 cut(s)	168
Fnu4HI	GCNGC	8 cut(s)	241, 349, 954, 1118, 1662, 2173, 2327, 2706
FokI	GGATG	6 cut(s)	218, 1805, 1826, 2357, 2384, 2569
FriOI	GRGCYC	3 cut(s)	31, 189, 1213
Fsp4HI	GCNGC	8 cut(s)	241, 349, 954, 1118, 1662, 2173, 2327, 2706
FspBI	CTAG	6 cut(s)	630, 785, 957, 1722, 1728, 2457
GlaI	GCGC	1 cut(s)	1160
GluI	GCNGC	8 cut(s)	241, 349, 954, 1118, 1662, 2173, 2327, 2706
GsaI	CCCAGC	2 cut(s)	1446, 1547
GsuI	CTGGAG	1 cut(s)	296
HaeIII	GGCC	8 cut(s)	570, 671, 1111, 1571, 1866, 2172, 2444, 2550
HapII	CCGG	3 cut(s)	41, 771, 839
HgaI	GACGC	2 cut(s)	232, 611
HhaI	GCGC	1 cut(s)	1161
Hin6I	GCGC	1 cut(s)	1159
HinP1I	GCGC	1 cut(s)	1159
HincII	GTYRAC	2 cut(s)	169, 658
HindII	GTYRAC	2 cut(s)	169, 658
HpaII	CCGG	3 cut(s)	41, 771, 839
HphI	GGTGA	7 cut(s)	94, 130, 158, 425, 444, 458, 1508
Hpy166II	GTNNAC	4 cut(s)	156, 169, 658, 1423
Hpy8I	GTNNAC	4 cut(s)	156, 169, 658, 1423
Hpy99I	CGWCG	3 cut(s)	173, 185, 230
HpyCH4III	ACNGT	4 cut(s)	1232, 1384, 1633, 2059
HpyCH4IV	ACGT	7 cut(s)	75, 128, 158, 1266, 1537, 1695, 2062
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1117, 1480, 2482
HpyF3I	CTNAG	9 cut(s)	62, 374, 648, 666, 681, 1113, 1944, 2472, 2571
HpySE526I	ACGT	7 cut(s)	75, 128, 158, 1266, 1537, 1695, 2062
HspAI	GCGC	1 cut(s)	1159
Kpn2I	TCCGGA	1 cut(s)	838
KroI	GCCGGC	1 cut(s)	40
KroNI	GCCGGC	1 cut(s)	42
Ksp22I	TGATCA	1 cut(s)	415
LguI	GCTCTTC	1 cut(s)	705
LmnI	GCTCC	2 cut(s)	192, 1208
Lsp1109I	GCAGC	5 cut(s)	227, 965, 1104, 1673, 2692
LweI	GCATC	4 cut(s)	1328, 1774, 2335, 2717
MabI	ACCWGGT	1 cut(s)	1308
MaeI	CTAG	6 cut(s)	630, 785, 957, 1722, 1728, 2457
MaeII	ACGT	7 cut(s)	75, 128, 158, 1266, 1537, 1695, 2062
MaeIII	GTNAC	5 cut(s)	732, 799, 937, 1589, 2141
MfeI	CAATTG	1 cut(s)	1491
MflI	RGATCY	5 cut(s)	34, 49, 868, 1295, 2459
MhlI	GDGCHC	6 cut(s)	31, 189, 1049, 1181, 1213, 2609
MlyI	GAGTC	5 cut(s)	174, 392, 1608, 2314, 2476
MmeI	TCCRAC	6 cut(s)	420, 459, 1440, 2023, 2306, 2330
Mph1103I	ATGCAT	1 cut(s)	391
MroI	TCCGGA	1 cut(s)	838
MroNI	GCCGGC	1 cut(s)	40
MroXI	GAANNNNTTC	1 cut(s)	2265
MslI	CAYNNNNRTG	1 cut(s)	1184
MspA1I	CMGCKG	2 cut(s)	1277, 1664
MspI	CCGG	3 cut(s)	41, 771, 839
MspR9I	CCNGG	7 cut(s)	771, 904, 1107, 1156, 1310, 1433, 1520
MunI	CAATTG	1 cut(s)	1491
Mva1269I	GAATGC	3 cut(s)	389, 2084, 2590
MvaI	CCWGG	6 cut(s)	904, 1107, 1156, 1310, 1433, 1520
MvnI	CGCG	1 cut(s)	738
MwoI	GCNNNNNNNGC	3 cut(s)	1117, 1480, 2482
NaeI	GCCGGC	1 cut(s)	42
NciI	CCSGG	1 cut(s)	771
NcoI	CCATGG	1 cut(s)	1594
NdeI	CATATG	2 cut(s)	943, 1063
NgoMIV	GCCGGC	1 cut(s)	40
NheI	GCTAGC	1 cut(s)	956
NlaIV	GGNNCC	4 cut(s)	305, 901, 1297, 2204
NmeAIII	GCCGAG	1 cut(s)	1192
NmuCI	GTSAC	2 cut(s)	1589, 2141
NsiI	ATGCAT	1 cut(s)	391
NspI	RCATGY	4 cut(s)	2142, 2569, 2631, 2682
PaeR7I	CTCGAG	1 cut(s)	30
PagI	TCATGA	1 cut(s)	2316
PciI	ACATGT	3 cut(s)	2138, 2627, 2678
PciSI	GCTCTTC	1 cut(s)	705
PcsI	WCGNNNNNNNCGW	3 cut(s)	177, 231, 240
PctI	GAATGC	3 cut(s)	389, 2084, 2590
PdiI	GCCGGC	1 cut(s)	42
PdmI	GAANNNNTTC	1 cut(s)	2265
Pfl23II	CGTACG	1 cut(s)	72
PflMI	CCANNNNNTGG	1 cut(s)	1315
PkrI	GCNGC	8 cut(s)	242, 350, 955, 1119, 1663, 2174, 2328, 2707
PleI	GAGTC	5 cut(s)	173, 392, 1607, 2314, 2475
PpsI	GAGTC	5 cut(s)	173, 392, 1607, 2314, 2475
Ppu21I	YACGTR	2 cut(s)	76, 1696
PpuMI	RGGWCCY	1 cut(s)	899
PscI	ACATGT	3 cut(s)	2138, 2627, 2678
PshBI	ATTAAT	1 cut(s)	965
Psp124BI	GAGCTC	2 cut(s)	189, 1213
Psp1406I	AACGTT	1 cut(s)	1266
Psp5II	RGGWCCY	1 cut(s)	899
Psp6I	CCWGG	6 cut(s)	902, 1105, 1154, 1308, 1431, 1518
PspFI	CCCAGC	2 cut(s)	1442, 1543
PspGI	CCWGG	6 cut(s)	902, 1105, 1154, 1308, 1431, 1518
PspLI	CGTACG	1 cut(s)	72
PspN4I	GGNNCC	4 cut(s)	305, 901, 1297, 2204
PspPPI	RGGWCCY	1 cut(s)	899
PstI	CTGCAG	2 cut(s)	2169, 2699
PstNI	CAGNNNCTG	1 cut(s)	2600
PsuI	RGATCY	5 cut(s)	34, 49, 868, 1295, 2459
PvuII	CAGCTG	2 cut(s)	1277, 1664
RseI	CAYNNNNRTG	1 cut(s)	1184
SacI	GAGCTC	2 cut(s)	189, 1213
SalI	GTCGAC	1 cut(s)	167
SapI	GCTCTTC	1 cut(s)	705
SatI	GCNGC	8 cut(s)	241, 349, 954, 1118, 1662, 2173, 2327, 2706
SbfI	CCTGCAGG	1 cut(s)	2169
ScaI	AGTACT	2 cut(s)	218, 544
SchI	GAGTC	5 cut(s)	174, 392, 1608, 2314, 2476
ScrFI	CCNGG	7 cut(s)	771, 904, 1107, 1156, 1310, 1433, 1520
SdaI	CCTGCAGG	1 cut(s)	2169
SduI	GDGCHC	6 cut(s)	31, 189, 1049, 1181, 1213, 2609
SexAI	ACCWGGT	1 cut(s)	1308
SfaNI	GCATC	4 cut(s)	1328, 1774, 2335, 2717
SfcI	CTRYAG	4 cut(s)	882, 2165, 2310, 2695
Sfr274I	CTCGAG	1 cut(s)	30
SinI	GGWCC	6 cut(s)	899, 991, 1306, 1838, 2620, 2691
SlaI	CTCGAG	1 cut(s)	30
SmiMI	CAYNNNNRTG	1 cut(s)	1184
SmlI	CTYRAG	3 cut(s)	30, 457, 1969
SmoI	CTYRAG	3 cut(s)	30, 457, 1969
Sse8387I	CCTGCAGG	1 cut(s)	2169
SsiI	CCGC	4 cut(s)	348, 402, 2173, 2326
SspI	AATATT	3 cut(s)	1367, 1773, 2673
SspMI	CTAG	6 cut(s)	630, 785, 957, 1722, 1728, 2457
SstI	GAGCTC	2 cut(s)	189, 1213
StyD4I	CCNGG	7 cut(s)	769, 902, 1105, 1154, 1308, 1431, 1518
StyI	CCWWGG	3 cut(s)	516, 1291, 1594
TaaI	ACNGT	4 cut(s)	1232, 1384, 1633, 2059
TaiI	ACGT	7 cut(s)	78, 131, 161, 1269, 1540, 1698, 2065
TaqI	TCGA	5 cut(s)	31, 163, 168, 270, 291
TatI	WGTACW	4 cut(s)	216, 377, 542, 1360
TauI	GCSGC	3 cut(s)	351, 2175, 2329
TscAI	CASTG	2 cut(s)	1387, 1594
TseFI	GTSAC	2 cut(s)	1589, 2141
TseI	GCWGC	5 cut(s)	240, 953, 1117, 1661, 2705
Tsp45I	GTSAC	2 cut(s)	1589, 2141
TspGWI	ACGGA	2 cut(s)	60, 1097
TspRI	CASTG	2 cut(s)	1387, 1594
Van91I	CCANNNNNTGG	1 cut(s)	1315
VpaK11BI	GGWCC	6 cut(s)	899, 991, 1306, 1838, 2620, 2691
VspI	ATTAAT	1 cut(s)	965
XagI	CCTNNNNNAGG	4 cut(s)	486, 1929, 2249, 2450
XapI	RAATTY	9 cut(s)	248, 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212
XbaI	TCTAGA	1 cut(s)	784
XceI	RCATGY	4 cut(s)	2142, 2569, 2631, 2682
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2096
XhoI	CTCGAG	1 cut(s)	30
XmiI	GTMKAC	1 cut(s)	168
XmnI	GAANNNNTTC	1 cut(s)	2265
XspI	CTAG	6 cut(s)	630, 785, 957, 1722, 1728, 2457
ZrmI	AGTACT	2 cut(s)	218, 544
Zsp2I	ATGCAT	1 cut(s)	391

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.