FvH4_3g03041

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011014
Physical Location & Seq
Forward (+)
1607881 .. 1612771
4891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03041.t3

Sequence Viewer

Length: 3174 bp
ATGCAGAGGGCTAGTAGTGCATCATGTTCAAGTAGTCGAGAAGATTCCAGCCATGATGCGGTGGGGGAAATACTTAGAAGTGTTTCTGTTGATAAGTTTAGTCATTTACAGAATGATAGTGAATCTGCTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAGAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCATTGGAAATATCCTTAAGGAAAAGGCGTAAAAAAGATTTACTCTCTGACAATGTGGCTGAGGGCCAGATTAAAGATGACCCTTCTCAATCTGGAAGAAGGAACAGGGTCACTCCTCCAGTCGGTAAAGAAAAAGATGACCTGTCTGCGGAGCTTAAAGAATTAGGGTGGTCCGATGAAGATGATAAAAAACAAGCGAGTCTGAGTTTGGAGGGTGAGCTCTCTTCTCTTCAGGGAGAAGTCTCACAAAAGAACAACAAGAGTACGGCTACTAGTGCCATTGACAAAACACAGGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGAAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTTGAAGAGCATGAATTTTTAGCTGAGGCTGAAGAGTCTGATGATGAAATATCTGCACTGATTCGTAGCATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAAGATGGCTTTAATTTCGACAGTCTTATTAATGTTGCTGATGATCATATTATAGATAGTAATTTTGAAGTGACAGATGAAGATATGGAGGACCCAGAAATAACTGCTGCCTTAAAATCTTTGGGATGGACTCAAGATTCGTACAATCCTCCTGAAACCTCTGCTCCCCAGATAGCTTTGGTTGACAAGGAAGCACTGTTAACTGAAATTCAATCCTTAAAAAGAGAGGCTTTAACTCATAAACGGGCAGGTAATGTCACAGAAGCAATGACACAGCTAAAGAAGGCAAAACTGCTTGAAAGGGACCTTGAAAACATCAAGTCTCAAAAGGGCAATGTCGTGAAACCTTCTGTAACAGTTCATAATCAAACTATTGATAGATCATCAAAGTCGTTGGGTGATGGAAATTTTAGTGCAATGGAACATATTGACATCAAACCTGCGAGGAAGAGTAAGTTGATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAAGGTCGATTAGATGAAGCTGAAGAAGAGTTGAAGAAAGGCAGAGTTCTCGAGCATCAGCTTGAAGAGATGGAAAATGGTAAGAACTCAGTTTTGGAACATCAGCATGCCAATGTTTCTGGAAGTCTGTCCGTTGCGGATGAGGAAGGAGACAATGTAACAGATCAAGATATGTATGACCCCGCATATCTTTCCATGCTAAAGAATTTGGGTTGGAATGATGATAATGAAGTTGCAAACTCTTTGTCAGAACCTTATAAGCAAATTGATAACATGCAGGCTACTGAATCTTCTGCAGCACAAGTCCCAAGTACGCTCCCGCTTGGAGGATCAAGGAAAAGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAAGCTCTTGCTTTGAGGCGCCAGGGAGACATGGAGGAAGCAGAAGAAGTGCTGAAAAAGGCAAAAGCATTAGAGGGTCAGTTGGCTGAGATGGAAGCACCAAAGAAAGTTCAATTGGACATCGCTAGGGACAAGGAAAATTTCATTGATCCTCCACTTGACAGTGTAGAGGAAAAAGGTGATGTCGGGGATGTAACAGAAAATGATATGCAGGACCCAGCACTACTGTCAGTGCTAAAGAGTTTGGGGTGGGAGGCTGAAGAGCATTCTGTAGATGCATCTCTTCCGCAATCTTCTTCCACACTTGCTGTTGCTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTCTGGATTTGAAAAGAAAGGCTCATGCGTTTAGACGAAAGGGACAGACTGAAGAAGCTGAAGAAGTGCTGAAGATGGCAAAGGTACTAGAAGTTCAGATTGAGGAGTTGGAAGCCCCAAAGGGTCTACCTATGGAATTGGGTCCTGGTCATCCAGAGAACCAGAGAATTGCTCAAGGTGCTACTCAATCTTCACCTGCCCAATCTGGGAACTTTGCAGATTTGTTGACAGGGGATGACTGGAGAGGTTCTCCAGGATCCGCTGAAAAACAATATGATAGCCTAACTTGCTCTGCTGATTCCGTTAATGCCAGTCCTCCAATTCAGCTGAGATCGTCACAGGAAGATCTTATAAAAAGAGATGATGCAATTATTCATAAACAGGAAGATACGGTTGTGGCCAATGAGAAGCGAGATGCTAAAGAGGCACATTTGGTTGTTAGACCTTCTTCACAGGAGAATGAAAGTGCCATTCGGCAAGAAATCATGTCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGGAAACTGACAGAAGCCCGGGAAGAACTTAAGCAGGCAAAAATGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTTGAGTGATATGTCGAGTCCTGCAAACAATGTTTCTCCAGCTGCGCAAAAGCAACATGGTTCACCGTCCTCTGGCCCGAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGACTGAAGAAGCGGAAGCAGAGTTTGAATTGGCCAAGGCACTTGAGGCTCAGTTAGAGGAAAGTGCTGCTCATGATTCCACAACTGTAGCACCGGTGGATGATGTGGCCGTAGAGGGACTTCTTGATCCTGAAATTCTTTCCGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACATCGTCTCAAGGTCCTGGAAGACCCGAGCCTTCAAAACCCAATGTTGGCAAGAATGACAATGTAATCCAAGATAGAAGTAATATAGAGGAACAGATCAAGGCAGAGAAGGGGAAGGCACTAAATCTGAAACGTGCTGGAAAACAAGCTGAGGCCTTGGATGCCCTTCGAAGAGCCAAGATGCTTGAAAAGAAGCTCAATTCCTCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1058

Amino Acids

116.94

Weight (kDa)

5.2

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 1.6e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1533, 2357
AarI CACCTGC 1 cut(s) 2209
AasI GACNNNNNNGTC 1 cut(s) 1278
Acc16I TGCGCA 1 cut(s) 2664
Acc36I ACCTGC 3 cut(s) 1001, 1210, 2209
AccB1I GGYRCC 1 cut(s) 1674
AccI GTMKAC 1 cut(s) 2131
AclWI GGATC 5 cut(s) 1612, 1799, 2256, 2269, 2906
AcoI YGGCCR 3 cut(s) 2403, 2817, 2892
AcsI RAATTY 8 cut(s) 180, 665, 969, 1168, 1480, 1626, 1795, 2919
AcyI GRCGYC 1 cut(s) 1675
AfaI GTAC 4 cut(s) 523, 905, 1588, 2091
AfiI CCNNNNNNNGG 7 cut(s) 58, 380, 406, 1601, 2211, 2690, 3008
AflII CTTAAG 3 cut(s) 274, 2558, 2932
AgeI ACCGGT 1 cut(s) 2878
AhlI ACTAGT 2 cut(s) 530, 2615
AjnI CCWGG 4 cut(s) 1677, 2149, 2257, 2977
AjuI GAANNNNNNNTTGG 6 cut(s) 1352, 1384, 1754, 1786, 1989, 2021
Alw21I GWGCWC 2 cut(s) 480, 1267
Alw26I GTCTC 7 cut(s) 505, 1089, 1264, 1419, 1677, 2745, 2973
AlwI GGATC 5 cut(s) 1612, 1799, 2256, 2269, 2906
Ama87I CYCGRG 3 cut(s) 1325, 2547, 2987
AoxI GGCC 7 cut(s) 322, 1257, 2403, 2692, 2817, 2892, 3114
ApeKI GCWGC 6 cut(s) 869, 1571, 1970, 2660, 2770, 2852
ApoI RAATTY 8 cut(s) 180, 665, 969, 1168, 1480, 1626, 1795, 2919
ArsI GACNNNNNNTTYG 8 cut(s) 883, 915, 1505, 1537, 2690, 2722, 2767, 2799
AseI ATTAAT 1 cut(s) 792
AsiGI ACCGGT 1 cut(s) 2878
Asp700I GAANNNNTTC 1 cut(s) 82
AspLEI GCGC 2 cut(s) 1677, 2665
AspS9I GGNCC 9 cut(s) 322, 429, 853, 1066, 1258, 1870, 2147, 2693, 2975
AsuC2I CCSGG 2 cut(s) 2548, 2549
AsuHPI GGTGA 6 cut(s) 485, 1172, 1847, 2000, 2190, 2673
AsuII TTCGAA 1 cut(s) 3130
AvaI CYCGRG 3 cut(s) 1325, 2547, 2987
AvaII GGWCC 6 cut(s) 429, 853, 1066, 1870, 2147, 2975
AxyI CCTNAGG 1 cut(s) 135
BaeI ACNNNNGTAYC 2 cut(s) 2385, 2418
BalI TGGCCA 2 cut(s) 2405, 2819
BamHI GGATCC 1 cut(s) 2261
BanI GGYRCC 1 cut(s) 1674
BanII GRGCYC 1 cut(s) 480
BarI GAAGNNNNNNTAC 2 cut(s) 2889, 2921
BbsI GAAGAC 2 cut(s) 2601, 2989
Bbv12I GWGCWC 2 cut(s) 480, 1267
BbvCI CCTCAGC 3 cut(s) 318, 675, 3111
BbvI GCAGC 6 cut(s) 856, 1583, 1957, 2647, 2757, 2839
BccI CCATC 6 cut(s) 761, 882, 1157, 1339, 1741, 2074
BceAI ACGGC 2 cut(s) 540, 2879
BcgI CGANNNNNNTGC 2 cut(s) 1306, 1340
BciT130I CCWGG 4 cut(s) 1679, 2151, 2259, 2979
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 2 cut(s) 2548, 2549
BcoDI GTCTC 7 cut(s) 505, 1089, 1264, 1419, 1677, 2745, 2973
BcuI ACTAGT 2 cut(s) 530, 2615
BfaI CTAG 7 cut(s) 12, 531, 641, 1782, 2093, 2616, 2744
BfmI CTRYAG 3 cut(s) 1569, 1926, 2871
BfoI RGCGCY 1 cut(s) 1678
BfrI CTTAAG 3 cut(s) 274, 2558, 2932
BfuAI ACCTGC 3 cut(s) 1001, 1210, 2209
BglII AGATCT 1 cut(s) 2350
BisI GCNGC 6 cut(s) 870, 1572, 1971, 2661, 2771, 2853
BlsI GCNGC 6 cut(s) 871, 1573, 1972, 2662, 2772, 2854
Bme1390I CCNGG 6 cut(s) 1679, 2151, 2259, 2548, 2549, 2979
Bme18I GGWCC 6 cut(s) 429, 853, 1066, 1870, 2147, 2975
BmeT110I CYCGRG 3 cut(s) 1325, 2547, 2987
BmgT120I GGNCC 9 cut(s) 322, 429, 853, 1066, 1258, 1870, 2147, 2693, 2975
BmiI GGNNCC 6 cut(s) 855, 1067, 1676, 1872, 2148, 2263
BmrFI CCNGG 6 cut(s) 1679, 2151, 2259, 2548, 2549, 2979
BpiI GAAGAC 2 cut(s) 2601, 2989
BplI GAGNNNNNCTC 4 cut(s) 2161, 2193, 2239, 2271
BpmI CTGGAG 5 cut(s) 360, 563, 2241, 2266, 2640
Bpu10I CCTNAGC 3 cut(s) 318, 675, 3111
Bpu14I TTCGAA 1 cut(s) 3130
BpuEI CTTGAG 6 cut(s) 263, 879, 2163, 2609, 2849, 2955
BpuMI CCSGG 2 cut(s) 2548, 2549
BsaHI GRCGYC 1 cut(s) 1675
BsaJI CCNNGG 6 cut(s) 612, 1678, 2547, 2820, 3117, 3165
BsaWI WCCGGW 1 cut(s) 2878
BsaXI ACNNNNNCTCC 4 cut(s) 910, 940, 1416, 1446
Bsc4I CCNNNNNNNGG 7 cut(s) 58, 380, 406, 1601, 2211, 2690, 3008
Bse118I RCCGGY 1 cut(s) 2878
Bse1I ACTGG 4 cut(s) 377, 2249, 2316, 2709
Bse21I CCTNAGG 1 cut(s) 135
Bse3DI GCAATG 3 cut(s) 1035, 1102, 1185
BseBI CCWGG 4 cut(s) 1679, 2151, 2259, 2979
BseDI CCNNGG 6 cut(s) 612, 1678, 2547, 2820, 3117, 3165
BseGI GGATG 9 cut(s) 166, 730, 893, 1420, 1852, 2155, 2245, 2890, 3127
BseLI CCNNNNNNNGG 7 cut(s) 58, 380, 406, 1601, 2211, 2690, 3008
BseMI GCAATG 3 cut(s) 1035, 1102, 1185
BseNI ACTGG 4 cut(s) 377, 2249, 2316, 2709
BseRI GAGGAG 3 cut(s) 363, 2123, 2518
BseXI GCAGC 6 cut(s) 856, 1583, 1957, 2647, 2757, 2839
BseYI CCCAGC 1 cut(s) 1873
BsgI GTGCAG 2 cut(s) 690, 1956
BshFI GGCC 7 cut(s) 324, 1259, 2405, 2694, 2819, 2894, 3116
BshNI GGYRCC 1 cut(s) 1674
BshTI ACCGGT 1 cut(s) 2878
BsiHKAI GWGCWC 2 cut(s) 480, 1267
BsiHKCI CYCGRG 3 cut(s) 1325, 2547, 2987
BsiSI CCGG 2 cut(s) 2548, 2879
BslFI GGGAC 5 cut(s) 1079, 1565, 1799, 2061, 2916
BslI CCNNNNNNNGG 7 cut(s) 58, 380, 406, 1601, 2211, 2690, 3008
BsmAI GTCTC 7 cut(s) 505, 1089, 1264, 1419, 1677, 2745, 2973
BsmBI CGTCTC 1 cut(s) 2973
BsmFI GGGAC 5 cut(s) 1079, 1565, 1799, 2061, 2916
BsmI GAATGC 1 cut(s) 1921
BsnI GGCC 7 cut(s) 324, 1259, 2405, 2694, 2819, 2894, 3116
BsoBI CYCGRG 3 cut(s) 1325, 2547, 2987
Bsp119I TTCGAA 1 cut(s) 3130
Bsp1286I GDGCHC 2 cut(s) 480, 1267
BspANI GGCC 7 cut(s) 324, 1259, 2405, 2694, 2819, 2894, 3116
BspHI TCATGA 1 cut(s) 2857
BspLI GGNNCC 6 cut(s) 855, 1067, 1676, 1872, 2148, 2263
BspMAI CTGCAG 1 cut(s) 1573
BspMI ACCTGC 3 cut(s) 1001, 1210, 2209
BspPI GGATC 5 cut(s) 1612, 1799, 2256, 2269, 2906
BspQI GCTCTTC 3 cut(s) 651, 1911, 3127
BspT104I TTCGAA 1 cut(s) 3130
BspT107I GGYRCC 1 cut(s) 1674
BspTI CTTAAG 3 cut(s) 274, 2558, 2932
BsrDI GCAATG 3 cut(s) 1035, 1102, 1185
BsrFI RCCGGY 1 cut(s) 2878
BsrI ACTGG 4 cut(s) 377, 2249, 2316, 2709
BssAI RCCGGY 1 cut(s) 2878
BssECI CCNNGG 6 cut(s) 612, 1678, 2547, 2820, 3117, 3165
BssNI GRCGYC 1 cut(s) 1675
BssT1I CCWWGG 3 cut(s) 612, 2820, 3117
Bst2UI CCWGG 4 cut(s) 1679, 2151, 2259, 2979
Bst4CI ACNGT 9 cut(s) 785, 960, 1120, 1820, 1884, 2398, 2600, 2685, 2872
BstACI GRCGYC 1 cut(s) 1675
BstAFI CTTAAG 3 cut(s) 274, 2558, 2932
BstBI TTCGAA 1 cut(s) 3130
BstC8I GCNNGC 6 cut(s) 153, 252, 603, 1383, 1554, 2565
BstF5I GGATG 9 cut(s) 166, 730, 893, 1420, 1852, 2155, 2245, 2890, 3127
BstH2I RGCGCY 1 cut(s) 1678
BstHHI GCGC 2 cut(s) 1677, 2665
BstMAI GTCTC 7 cut(s) 505, 1089, 1264, 1419, 1677, 2745, 2973
BstNI CCWGG 4 cut(s) 1679, 2151, 2259, 2979
BstNSI RCATGY 2 cut(s) 1385, 1552
BstSCI CCNGG 6 cut(s) 1677, 2149, 2257, 2546, 2547, 2977
BstSFI CTRYAG 3 cut(s) 1569, 1926, 2871
BstV1I GCAGC 6 cut(s) 856, 1583, 1957, 2647, 2757, 2839
BstV2I GAAGAC 2 cut(s) 2601, 2989
BstX2I RGATCY 2 cut(s) 2261, 2350
BstYI RGATCY 2 cut(s) 2261, 2350
Bsu36I CCTNAGG 1 cut(s) 135
BsuRI GGCC 7 cut(s) 324, 1259, 2405, 2694, 2819, 2894, 3116
BtgZI GCGATG 1 cut(s) 1762
BtsCI GGATG 9 cut(s) 166, 730, 893, 1420, 1852, 2155, 2245, 2890, 3127
BtsIMutI CAGTG 4 cut(s) 707, 956, 1825, 1893
BveI ACCTGC 3 cut(s) 1001, 1210, 2209
Cac8I GCNNGC 6 cut(s) 153, 252, 603, 1383, 1554, 2565
CciI TCATGA 1 cut(s) 2857
CfoI GCGC 2 cut(s) 1677, 2665
Cfr10I RCCGGY 1 cut(s) 2878
Cfr13I GGNCC 9 cut(s) 322, 429, 853, 1066, 1258, 1870, 2147, 2693, 2975
Cfr9I CCCGGG 1 cut(s) 2547
CseI GACGC 2 cut(s) 134, 2573
Csp6I GTAC 4 cut(s) 522, 904, 1587, 2090
CspAI ACCGGT 1 cut(s) 2878
CviQI GTAC 4 cut(s) 522, 904, 1587, 2090
DinI GGCGCC 1 cut(s) 1676
DrdI GACNNNNNNGTC 1 cut(s) 1278
DseDI GACNNNNNNGTC 1 cut(s) 1278
EaeI YGGCCR 3 cut(s) 2403, 2817, 2892
EciI GGCGGA 1 cut(s) 2917
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 3 cut(s) 612, 2820, 3117
Eco147I AGGCCT 1 cut(s) 3116
Eco24I GRGCYC 1 cut(s) 480
Eco47I GGWCC 6 cut(s) 429, 853, 1066, 1870, 2147, 2975
Eco53kI GAGCTC 1 cut(s) 478
Eco81I CCTNAGG 1 cut(s) 135
Eco88I CYCGRG 3 cut(s) 1325, 2547, 2987
EcoICRI GAGCTC 1 cut(s) 478
EcoO109I RGGNCCY 5 cut(s) 853, 1066, 1870, 2147, 2975
EcoRII CCWGG 4 cut(s) 1677, 2149, 2257, 2977
EcoT14I CCWWGG 3 cut(s) 612, 2820, 3117
EcoT22I ATGCAT 2 cut(s) 262, 1936
EcoT38I GRGCYC 1 cut(s) 480
EgeI GGCGCC 1 cut(s) 1676
EheI GGCGCC 1 cut(s) 1676
ErhI CCWWGG 3 cut(s) 612, 2820, 3117
Esp3I CGTCTC 1 cut(s) 2973
FalI AAGNNNNNCTT 2 cut(s) 976, 1008
FaqI GGGAC 5 cut(s) 1079, 1565, 1799, 2061, 2916
FauI CCCGC 2 cut(s) 1465, 1602
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 2131
Fnu4HI GCNGC 6 cut(s) 870, 1572, 1971, 2661, 2771, 2853
FokI GGATG 9 cut(s) 173, 737, 900, 1427, 1859, 2142, 2252, 2897, 3134
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 6 cut(s) 870, 1572, 1971, 2661, 2771, 2853
FspBI CTAG 7 cut(s) 12, 531, 641, 1782, 2093, 2616, 2744
FspI TGCGCA 1 cut(s) 2664
GlaI GCGC 2 cut(s) 1676, 2664
GluI GCNGC 6 cut(s) 870, 1572, 1971, 2661, 2771, 2853
GsaI CCCAGC 1 cut(s) 1877
GsuI CTGGAG 5 cut(s) 360, 563, 2241, 2266, 2640
HaeII RGCGCY 1 cut(s) 1678
HaeIII GGCC 7 cut(s) 324, 1259, 2405, 2694, 2819, 2894, 3116
HapII CCGG 2 cut(s) 2548, 2879
HgaI GACGC 2 cut(s) 134, 2573
HhaI GCGC 2 cut(s) 1677, 2665
Hin1I GRCGYC 1 cut(s) 1675
Hin6I GCGC 2 cut(s) 1675, 2663
HinP1I GCGC 2 cut(s) 1675, 2663
HincII GTYRAC 3 cut(s) 946, 963, 2232
HindII GTYRAC 3 cut(s) 946, 963, 2232
HindIII AAGCTT 2 cut(s) 153, 599
HpaI GTTAAC 1 cut(s) 963
HpaII CCGG 2 cut(s) 2548, 2879
HphI GGTGA 6 cut(s) 485, 1172, 1847, 2000, 2190, 2673
Hpy166II GTNNAC 5 cut(s) 946, 963, 2132, 2232, 2681
Hpy8I GTNNAC 5 cut(s) 946, 963, 2132, 2232, 2681
HpyCH4III ACNGT 9 cut(s) 785, 960, 1120, 1820, 1884, 2398, 2600, 2685, 2872
HpyCH4IV ACGT 2 cut(s) 2755, 3094
HpySE526I ACGT 2 cut(s) 2755, 3094
Hsp92I GRCGYC 1 cut(s) 1675
HspAI GCGC 2 cut(s) 1675, 2663
KasI GGCGCC 1 cut(s) 1674
Ksp22I TGATCA 1 cut(s) 805
KspAI GTTAAC 1 cut(s) 963
LguI GCTCTTC 3 cut(s) 651, 1911, 3127
LmnI GCTCC 5 cut(s) 238, 409, 931, 1596, 2002
Lsp1109I GCAGC 6 cut(s) 856, 1583, 1957, 2647, 2757, 2839
MaeI CTAG 7 cut(s) 12, 531, 641, 1782, 2093, 2616, 2744
MaeII ACGT 2 cut(s) 2755, 3094
MaeIII GTNAC 8 cut(s) 367, 832, 1018, 1114, 1432, 1849, 2340, 2747
MfeI CAATTG 2 cut(s) 1769, 2940
MflI RGATCY 2 cut(s) 2261, 2350
MhlI GDGCHC 2 cut(s) 480, 1267
MlsI TGGCCA 2 cut(s) 2405, 2819
MluNI TGGCCA 2 cut(s) 2405, 2819
Mly113I GGCGCC 1 cut(s) 1675
MlyI GAGTC 5 cut(s) 466, 695, 886, 2644, 2747
MmeI TCCRAC 4 cut(s) 1469, 2094, 2556, 2765
Mox20I TGGCCA 2 cut(s) 2405, 2819
Mph1103I ATGCAT 2 cut(s) 262, 1936
MroXI GAANNNNTTC 1 cut(s) 82
MscI TGGCCA 2 cut(s) 2405, 2819
MslI CAYNNNNRTG 2 cut(s) 1380, 1386
Msp20I TGGCCA 2 cut(s) 2405, 2819
MspA1I CMGCKG 4 cut(s) 583, 2267, 2332, 2660
MspCI CTTAAG 3 cut(s) 274, 2558, 2932
MspI CCGG 2 cut(s) 2548, 2879
MspR9I CCNGG 6 cut(s) 1679, 2151, 2259, 2548, 2549, 2979
MunI CAATTG 2 cut(s) 1769, 2940
Mva1269I GAATGC 1 cut(s) 1921
MvaI CCWGG 4 cut(s) 1679, 2151, 2259, 2979
NarI GGCGCC 1 cut(s) 1675
NciI CCSGG 2 cut(s) 2548, 2549
NlaIV GGNNCC 6 cut(s) 855, 1067, 1676, 1872, 2148, 2263
NmeAIII GCCGAG 1 cut(s) 3146
NmuCI GTSAC 5 cut(s) 367, 832, 1018, 2340, 2747
NsbI TGCGCA 1 cut(s) 2664
NsiI ATGCAT 2 cut(s) 262, 1936
NspI RCATGY 2 cut(s) 1385, 1552
NspV TTCGAA 1 cut(s) 3130
PaeI GCATGC 1 cut(s) 1385
PaeR7I CTCGAG 1 cut(s) 1325
PagI TCATGA 1 cut(s) 2857
PaqCI CACCTGC 1 cut(s) 2209
PceI AGGCCT 1 cut(s) 3116
PciSI GCTCTTC 3 cut(s) 651, 1911, 3127
PctI GAATGC 1 cut(s) 1921
PdmI GAANNNNTTC 1 cut(s) 82
PfeI GAWTC 8 cut(s) 44, 122, 712, 899, 1225, 1562, 2303, 2861
PfoI TCCNGGA 2 cut(s) 2257, 2977
PinAI ACCGGT 1 cut(s) 2878
PkrI GCNGC 6 cut(s) 871, 1573, 1972, 2662, 2772, 2854
PleI GAGTC 5 cut(s) 465, 694, 886, 2643, 2746
PluTI GGCGCC 1 cut(s) 1678
PpsI GAGTC 5 cut(s) 465, 694, 886, 2643, 2746
PpuMI RGGWCCY 5 cut(s) 853, 1066, 1870, 2147, 2975
PshBI ATTAAT 1 cut(s) 792
PsiI TTATAA 2 cut(s) 1533, 2357
Psp124BI GAGCTC 1 cut(s) 480
Psp5II RGGWCCY 5 cut(s) 853, 1066, 1870, 2147, 2975
Psp6I CCWGG 4 cut(s) 1677, 2149, 2257, 2977
PspFI CCCAGC 1 cut(s) 1873
PspGI CCWGG 4 cut(s) 1677, 2149, 2257, 2977
PspN4I GGNNCC 6 cut(s) 855, 1067, 1676, 1872, 2148, 2263
PspPI GGNCC 9 cut(s) 322, 429, 853, 1066, 1258, 1870, 2147, 2693, 2975
PspPPI RGGWCCY 5 cut(s) 853, 1066, 1870, 2147, 2975
PsrI GAACNNNNNNTAC 2 cut(s) 2082, 2114
PstI CTGCAG 1 cut(s) 1573
PsuI RGATCY 2 cut(s) 2261, 2350
PvuII CAGCTG 3 cut(s) 583, 2332, 2660
RsaI GTAC 4 cut(s) 523, 905, 1588, 2091
RsaNI GTAC 4 cut(s) 522, 904, 1587, 2090
RseI CAYNNNNRTG 2 cut(s) 1380, 1386
SacI GAGCTC 1 cut(s) 480
SapI GCTCTTC 3 cut(s) 651, 1911, 3127
SatI GCNGC 6 cut(s) 870, 1572, 1971, 2661, 2771, 2853
Sau96I GGNCC 9 cut(s) 322, 429, 853, 1066, 1258, 1870, 2147, 2693, 2975
SchI GAGTC 5 cut(s) 466, 695, 886, 2644, 2747
ScrFI CCNGG 6 cut(s) 1679, 2151, 2259, 2548, 2549, 2979
SduI GDGCHC 2 cut(s) 480, 1267
SfcI CTRYAG 3 cut(s) 1569, 1926, 2871
SfoI GGCGCC 1 cut(s) 1676
Sfr274I CTCGAG 1 cut(s) 1325
SfuI TTCGAA 1 cut(s) 3130
SgrAI CRCCGGYG 1 cut(s) 2878
SinI GGWCC 6 cut(s) 429, 853, 1066, 1870, 2147, 2975
SlaI CTCGAG 1 cut(s) 1325
SmaI CCCGGG 1 cut(s) 2549
SmiMI CAYNNNNRTG 2 cut(s) 1380, 1386
SpeI ACTAGT 2 cut(s) 530, 2615
SphI GCATGC 1 cut(s) 1385
SseBI AGGCCT 1 cut(s) 3116
SspDI GGCGCC 1 cut(s) 1674
SspMI CTAG 7 cut(s) 12, 531, 641, 1782, 2093, 2616, 2744
SstI GAGCTC 1 cut(s) 480
StuI AGGCCT 1 cut(s) 3116
StyD4I CCNGG 6 cut(s) 1677, 2149, 2257, 2546, 2547, 2977
StyI CCWWGG 3 cut(s) 612, 2820, 3117
TaaI ACNGT 9 cut(s) 785, 960, 1120, 1820, 1884, 2398, 2600, 2685, 2872
TaiI ACGT 2 cut(s) 2758, 3097
TaqI TCGA 6 cut(s) 37, 780, 1282, 1326, 2633, 3130
TfiI GAWTC 8 cut(s) 44, 122, 712, 899, 1225, 1562, 2303, 2861
TscAI CASTG 4 cut(s) 714, 963, 1825, 1893
TseFI GTSAC 5 cut(s) 367, 832, 1018, 2340, 2747
TseI GCWGC 6 cut(s) 869, 1571, 1970, 2660, 2770, 2852
Tsp45I GTSAC 5 cut(s) 367, 832, 1018, 2340, 2747
TspGWI ACGGA 2 cut(s) 1396, 2296
TspMI CCCGGG 1 cut(s) 2547
TspRI CASTG 4 cut(s) 714, 963, 1825, 1893
Vha464I CTTAAG 3 cut(s) 274, 2558, 2932
VpaK11BI GGWCC 6 cut(s) 429, 853, 1066, 1870, 2147, 2975
VspI ATTAAT 1 cut(s) 792
XapI RAATTY 8 cut(s) 180, 665, 969, 1168, 1480, 1626, 1795, 2919
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 2 cut(s) 1385, 1552
XhoI CTCGAG 1 cut(s) 1325
XmaI CCCGGG 1 cut(s) 2547
XmiI GTMKAC 1 cut(s) 2131
XmnI GAANNNNTTC 1 cut(s) 82
XspI CTAG 7 cut(s) 12, 531, 641, 1782, 2093, 2616, 2744
Zsp2I ATGCAT 2 cut(s) 262, 1936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.