Rw5G003950

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011014
Physical Location & Seq
Forward (+)
3923777 .. 3927933
4157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003950.1

Sequence Viewer

Length: 3642 bp
ATGCTGGAGAAGATCGGGTTGCCGGCGAAGCCATCACTGAGAGGGAATAATTGGGTGGTTGACGCCTCACACTGTCAGGGATGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCATCATTGCCGGAGGTGTGGGGGCATTTTCTGTGGCAGCTGTACACAGCAGAGGATGTTTTTGCGTGGGCAAGGCGATGCGCCTGTGCGGATATGTGAGCCTTGTAAGAAGCTAGAAGAAGCAGCGCGGTTCGAGAGATATGGACACAAGAGTAGAGCTGCGAGAGGCAGTTCAAATTTGACATCAAAGCCTGAGGATGAGATTTTGAACCAGATACTTGGTAATGAAGGGAAGGAATCAGGACAAGAGGTTAACAAGAATGTAGTTTCCAGTATGCTGAGGGCAACTAGCAGTGCATCATGTTCAAATAGTCGAGGAGATTCCAGCCATGATGCGGTGGGAGAAATACTTAGAAGCGTTTCTGTTGATAAGTTTAGTCAATTACAGAATGATAGTGAATCTGTTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAAAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCGTTGGAAATATCCTTAAGGAAAAAGCGTAAAAAGGATTTACTCTCTGACAATGTGGCTGAGGGCCAGATTAAAGATGACTCTTCCCAATCTGGAAGAAGAAACAGGGTCACTCCTCCAGTGGGTAAAGAAAAAGATGACCTTTCAGCGGAGCTTAAAGAATTAGGGTGGTCTGATATGGATCTCCGTGACGAGAATAAAAAACAAGCGAGTCTGACTTTGGAGGGCGAGCTGTCTTCTCTTCTGGGAGAAATCTCACAAAAGAGCAATAAAAGTAAGGCTACTAGTGCCATTGACAAAACACAGGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGCAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTTGAAGAACAGGAATTGTTGGGTGAGGCTGAAGAGTCCGATGATGAAATATCTGCACTGATTCGTAGTATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAGGATGGCTTTAATTTTGATAGTCTTATTAATGCTGCTGATGATCATATTATAGATAGTAATTTTGAAGTGACAGAAGAGGATATGGAGGACCCAGAAATAACTGCTGCCTTAAAATCTTTAGGATGGACTCAAGATTCAAATAATCCTCCTGAAACCTCTGCTACCCAGATAGCTTCAGTTGACAAGGAAGCACTATTAAGTGAAATTCAATCCTTAAAACGAGAGGCTGTAACTCTTAAACGAGCAGGTAATGCCACAGAAGCAATGGTGCAGCTAAAAAAGGCAAAACTGCTTGAAAGGGACCTTGAAAGCATTGAGTCCCAAGAGGGCAATGTCGTGAAACCTTCTGCAACAGTTCATAATCAAACTATTGATAGATCATCAAAGTCATCTATGGTGGATGATGGAAATATTAGTGCAATGGAACATATTGACTTCAAACCTGCGAGGAAGAGTAAGTTAATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAGGGACGATTAGATGAAGCTGAAGAAGAGTTGAAGAGAGGCAGGATTCTTGAGCATCAGCTTGAAGAGATGGAGAATGGTAAGGACTCAGTTTTGGAACATGAGCATGCCAATGTCTCTGGAAGTCTGTCTTTTGCAGATGAGGAAGGAGAGAATGTAACAGATCAAGATATGTATGATCCAACATATCTTTCCATGCTAAGGAATTTGGGTTGGAATGATGATAATGAAGTTGCAAACTCTTCATCAGCACCTGATAAGAAAATTGATAACATGCAGGCTAGTGAATCTTCTGGAGCACAAGCCCGAAGTGATTTCCCGGTTGGAGGATCAAGGAAAAGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAGGCTCTTGCTCTGAGGCGCCAGGGAGACACCGAGGAAGCAGAAGAAATGCTGAAAAAGGCAAAAGCATTAGAGGGGGAGATGGCGGAGATGGAAGCACCGAAGAAAGTTCAGTCGGACTTTGGTAGGGACAAGGACAATTTCATTGATCCTATTCCTTTCAGTGTAGAGGAAGAAGGTGATGGCGGGGATGTAACAGAAAATGATATGCAGGACCCAGCACTACTCTCAGTGCTAAAGAGTTTGGGGTGGGAGGATAAAGAGCATTCTGTAGAGGCATCTCCTCTGCAATCTTCTTCCAAACTTGCTGTTGCTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTTTGGATTTGAAAAGAAAGGCTCATGCATTTAGACGAAAGGGGCAGACTGAAGAAGCTGAAGAAGTGTTGAAGATGGCAAAGGTACTAGAAGTTCAAATTGAAGAGTTGGAAGCCCCAAAAGGTTTACCAATGGAATTGGGTCCAGGTCGTCCAGACAACCAGAGAATTGCTCAAGGCGCTAGTCAGTCTTCACCTGCCCAATCTGGGAACTTCGCAGATTTGTTGACGGGGGATGACTGGAGAGGTTCTCCAGGATCTGCTGAAAAACAATATGATAACCTATCTTATTCTGTTGATTCTGTTAATGCCAGTCCTCCCATTCAGTTGAGATCCTCACAAGAAGATCTGAGAAAAAGAGATGATGCAATAATTAATAAACGGGAAGATACGATTGTAGCCAATGAGAAGCAAGGTGCTAAAGAGGTCAATTTGGTTCTGGGATCTTCTTCACAGAAGAATGAAAGTGCCAATGAGAAGCAAGATGCTACAGAGGTCAATTTGGTTCTGGGATCATCCTCACAGAAGAATGAAAGTGCCATTCGGCAAGAAATCGTGGCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGAAAACTGACAGAAGCCCGCGAAGAACTTAGGCAGGCTAAAGTGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTCGAGTGATGTGTCCGGTTCTGCTAACAATGTTCCTCCAGCTGCCCAAAAGCAGCATGGATCACCGTCCTCGGGCCAAAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGATTGAAGAAGCGGAAGCAGAGTTTGAATTGGCCAAGGTACTTGAGGCTCAGTTAGAGGAAAGTGCTACTCATGATTCCACTAGTGCAGCACCAGTGGATGATGCGGCCGTAGAGGGACTTCTTGATCCTGAACTTCTGTCTGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACATCATCTCAAGGTCCTGGAAGACCAGAGCATTCAAAACCCAACGATGTAATCCAAGATAGAAGTAATCTAGAGGAACAGATCAAGGCAGAGAAGGTGAAGGCACTAAATCTGAAACGTGCAGGAAAACAAGCCGAGGCCTTGGATGCTCTTCGAAGGGCCAAGATGCTTGAAAAGAAGCTGAATTCCTCGGCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1213

Amino Acids

134.0

Weight (kDa)

5.35

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 1.6e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2617
Acc36I ACCTGC 3 cut(s) 1403, 1618, 2617
AccB1I GGYRCC 1 cut(s) 2082
AccII CGCG 2 cut(s) 241, 3027
AcoI YGGCCR 2 cut(s) 3294, 3369
AcsI RAATTY 6 cut(s) 289, 570, 1371, 1888, 2034, 3622
AcuI CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3263
AcyI GRCGYC 2 cut(s) 63, 2083
AfaI GTAC 3 cut(s) 157, 2499, 3303
AfiI CCNNNNNNNGG 7 cut(s) 448, 770, 796, 1884, 2009, 2619, 3167
AflII CTTAAG 2 cut(s) 664, 3409
AhlI ACTAGT 3 cut(s) 932, 3092, 3344
AjnI CCWGG 4 cut(s) 2085, 2557, 2665, 3454
AjuI GAANNNNNNNTTGG 2 cut(s) 1858, 1890
Alw21I GWGCWC 2 cut(s) 1675, 1984
Alw26I GTCTC 4 cut(s) 1672, 1804, 2085, 3222
AlwNI CAGNNNCTG 2 cut(s) 1937, 2672
Ama87I CYCGRG 1 cut(s) 3166
AoxI GGCC 7 cut(s) 712, 1665, 3169, 3294, 3369, 3576, 3597
ApoI RAATTY 6 cut(s) 289, 570, 1371, 1888, 2034, 3622
ArsI GACNNNNNNTTYG 2 cut(s) 3244, 3276
AseI ATTAAT 2 cut(s) 1194, 2787
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 4 cut(s) 196, 241, 2085, 2594
AspS9I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3169, 3452, 3597
AsuC2I CCSGG 1 cut(s) 2003
AsuHPI GGTGA 7 cut(s) 88, 1088, 2255, 2408, 2598, 3150, 3547
AsuII TTCGAA 1 cut(s) 3592
AvaI CYCGRG 1 cut(s) 3166
AvaII GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3452
AxyI CCTNAGG 2 cut(s) 306, 525
BaeI ACNNNNGTAYC 2 cut(s) 2793, 2826
BalI TGGCCA 1 cut(s) 3296
BanI GGYRCC 1 cut(s) 2082
BarI GAAGNNNNNNTAC 2 cut(s) 3366, 3398
BbsI GAAGAC 4 cut(s) 876, 2595, 3078, 3466
Bbv12I GWGCWC 2 cut(s) 1675, 1984
BbvCI CCTCAGC 2 cut(s) 392, 708
BccI CCATC 9 cut(s) 40, 1163, 1284, 1565, 1747, 2140, 2149, 2240, 2482
BceAI ACGGC 1 cut(s) 3356
BciT130I CCWGG 4 cut(s) 2087, 2559, 2667, 3456
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 2003
BcoDI GTCTC 4 cut(s) 1672, 1804, 2085, 3222
BcuI ACTAGT 3 cut(s) 932, 3092, 3344
BfmI CTRYAG 2 cut(s) 2334, 2901
BfoI RGCGCY 2 cut(s) 2086, 2595
BfrI CTTAAG 2 cut(s) 664, 3409
BfuAI ACCTGC 3 cut(s) 1403, 1618, 2617
BglII AGATCT 1 cut(s) 2758
Bme1390I CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3456
Bme18I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3452
BmeT110I CYCGRG 1 cut(s) 3166
BmgT120I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3169, 3452, 3597
BmiI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3633
BmrFI CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3456
BpiI GAAGAC 4 cut(s) 876, 2595, 3078, 3466
BplI GAGNNNNNCTC 4 cut(s) 2569, 2601, 2647, 2679
BpmI CTGGAG 7 cut(s) 26, 750, 965, 1998, 2649, 2674, 3117
Bpu10I CCTNAGC 3 cut(s) 392, 708, 1883
Bpu14I TTCGAA 1 cut(s) 3592
BpuEI CTTGAG 7 cut(s) 653, 1281, 1754, 2571, 3086, 3326, 3432
BpuMI CCSGG 1 cut(s) 2003
BsaBI GATNNNNATC 1 cut(s) 828
BsaHI GRCGYC 2 cut(s) 63, 2083
BsaJI CCNNGG 8 cut(s) 1014, 2086, 2097, 3165, 3297, 3573, 3579, 3627
BsaWI WCCGGW 1 cut(s) 3110
BsaXI ACNNNNNCTCC 2 cut(s) 1824, 1854
Bsc4I CCNNNNNNNGG 7 cut(s) 448, 770, 796, 1884, 2009, 2619, 3167
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 6 cut(s) 384, 767, 2657, 2724, 3186, 3356
Bse21I CCTNAGG 2 cut(s) 306, 525
Bse3DI GCAATG 4 cut(s) 118, 1437, 1504, 1593
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 4 cut(s) 2087, 2559, 2667, 3456
BseDI CCNNGG 8 cut(s) 1014, 2086, 2097, 3165, 3297, 3573, 3579, 3627
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 7 cut(s) 448, 770, 796, 1884, 2009, 2619, 3167
BseMI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BseNI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3186, 3356
BseRI GAGGAG 4 cut(s) 444, 753, 2337, 2995
BseX3I CGGCCG 1 cut(s) 3369
BseYI CCCAGC 1 cut(s) 2281
BsgI GTGCAG 5 cut(s) 1092, 1457, 2364, 3369, 3579
Bsh1236I CGCG 2 cut(s) 241, 3027
Bsh1285I CGRYCG 1 cut(s) 3372
BshFI GGCC 7 cut(s) 714, 1667, 3171, 3296, 3371, 3578, 3599
BshNI GGYRCC 1 cut(s) 2082
BsiEI CGRYCG 1 cut(s) 3372
BsiHKAI GWGCWC 2 cut(s) 1675, 1984
BsiHKCI CYCGRG 1 cut(s) 3166
BsiSI CCGG 4 cut(s) 23, 124, 2003, 3111
BslFI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3393
BslI CCNNNNNNNGG 7 cut(s) 448, 770, 796, 1884, 2009, 2619, 3167
BsmAI GTCTC 4 cut(s) 1672, 1804, 2085, 3222
BsmFI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3393
BsmI GAATGC 2 cut(s) 2329, 3469
BsnI GGCC 7 cut(s) 714, 1667, 3171, 3296, 3371, 3578, 3599
BsoBI CYCGRG 1 cut(s) 3166
Bsp119I TTCGAA 1 cut(s) 3592
Bsp1286I GDGCHC 2 cut(s) 1675, 1984
Bsp1407I TGTACA 1 cut(s) 155
BspANI GGCC 7 cut(s) 714, 1667, 3171, 3296, 3371, 3578, 3599
BspFNI CGCG 2 cut(s) 241, 3027
BspHI TCATGA 1 cut(s) 3334
BspLI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3633
BspMI ACCTGC 3 cut(s) 1403, 1618, 2617
BspQI GCTCTTC 1 cut(s) 3594
BspT104I TTCGAA 1 cut(s) 3592
BspT107I GGYRCC 1 cut(s) 2082
BspTI CTTAAG 2 cut(s) 664, 3409
BsrDI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BsrFI RCCGGY 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 155
BsrI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3186, 3356
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 8 cut(s) 1014, 2086, 2097, 3165, 3297, 3573, 3579, 3627
BssNI GRCGYC 2 cut(s) 63, 2083
BssT1I CCWWGG 3 cut(s) 1014, 3297, 3579
Bst2UI CCWGG 4 cut(s) 2087, 2559, 2667, 3456
Bst4CI ACNGT 4 cut(s) 74, 1522, 3077, 3162
BstACI GRCGYC 2 cut(s) 63, 2083
BstAFI CTTAAG 2 cut(s) 664, 3409
BstAPI GCANNNNNTGC 1 cut(s) 1418
BstAUI TGTACA 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 3592
BstFNI CGCG 2 cut(s) 241, 3027
BstH2I RGCGCY 2 cut(s) 2086, 2595
BstHHI GCGC 4 cut(s) 196, 241, 2085, 2594
BstMAI GTCTC 4 cut(s) 1672, 1804, 2085, 3222
BstMCI CGRYCG 1 cut(s) 3372
BstNI CCWGG 4 cut(s) 2087, 2559, 2667, 3456
BstNSI RCATGY 2 cut(s) 1793, 1960
BstSCI CCNGG 5 cut(s) 2001, 2085, 2557, 2665, 3454
BstSFI CTRYAG 2 cut(s) 2334, 2901
BstUI CGCG 2 cut(s) 241, 3027
BstV2I GAAGAC 4 cut(s) 876, 2595, 3078, 3466
BstX2I RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstZI CGGCCG 1 cut(s) 3369
Bsu36I CCTNAGG 2 cut(s) 306, 525
BsuRI GGCC 7 cut(s) 714, 1667, 3171, 3296, 3371, 3578, 3599
BtgZI GCGATG 1 cut(s) 204
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 8 cut(s) 35, 70, 412, 774, 1109, 2233, 2301, 3363
BveI ACCTGC 3 cut(s) 1403, 1618, 2617
CaiI CAGNNNCTG 2 cut(s) 1937, 2672
CciI TCATGA 1 cut(s) 3334
CfoI GCGC 4 cut(s) 196, 241, 2085, 2594
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3169, 3452, 3597
CseI GACGC 3 cut(s) 71, 524, 3050
Csp6I GTAC 3 cut(s) 156, 2498, 3302
CviAII CATG 9 cut(s) 414, 443, 1784, 1790, 1879, 1957, 2438, 3152, 3335
CviQI GTAC 3 cut(s) 156, 2498, 3302
DinI GGCGCC 1 cut(s) 2084
EaeI YGGCCR 2 cut(s) 3294, 3369
EagI CGGCCG 1 cut(s) 3369
EciI GGCGGA 1 cut(s) 2165
EclXI CGGCCG 1 cut(s) 3369
Eco130I CCWWGG 3 cut(s) 1014, 3297, 3579
Eco147I AGGCCT 1 cut(s) 3578
Eco47I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3452
Eco52I CGGCCG 1 cut(s) 3369
Eco57I CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3263
Eco81I CCTNAGG 2 cut(s) 306, 525
Eco88I CYCGRG 1 cut(s) 3166
EcoO109I RGGNCCY 4 cut(s) 1255, 1468, 2278, 3452
EcoRI GAATTC 1 cut(s) 3622
EcoRII CCWGG 4 cut(s) 2085, 2557, 2665, 3454
EcoT14I CCWWGG 3 cut(s) 1014, 3297, 3579
EcoT22I ATGCAT 1 cut(s) 2443
EgeI GGCGCC 1 cut(s) 2084
EheI GGCGCC 1 cut(s) 2084
ErhI CCWWGG 3 cut(s) 1014, 3297, 3579
FaeI CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3155, 3338
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1798, 1830
FaqI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3393
FatI CATG 9 cut(s) 413, 442, 1783, 1789, 1878, 1956, 2437, 3151, 3334
FauI CCCGC 2 cut(s) 2243, 3032
FbaI TGATCA 1 cut(s) 1207
GlaI GCGC 4 cut(s) 195, 240, 2084, 2593
GsaI CCCAGC 1 cut(s) 2285
GsuI CTGGAG 7 cut(s) 26, 750, 965, 1998, 2649, 2674, 3117
HaeII RGCGCY 2 cut(s) 2086, 2595
HaeIII GGCC 7 cut(s) 714, 1667, 3171, 3296, 3371, 3578, 3599
HapII CCGG 4 cut(s) 23, 124, 2003, 3111
HgaI GACGC 3 cut(s) 71, 524, 3050
HhaI GCGC 4 cut(s) 196, 241, 2085, 2594
Hin1I GRCGYC 2 cut(s) 63, 2083
Hin1II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3155, 3338
Hin6I GCGC 4 cut(s) 194, 239, 2083, 2592
HinP1I GCGC 4 cut(s) 194, 239, 2083, 2592
HincII GTYRAC 4 cut(s) 61, 367, 1348, 2640
HindII GTYRAC 4 cut(s) 61, 367, 1348, 2640
HindIII AAGCTT 2 cut(s) 543, 1001
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 4 cut(s) 23, 124, 2003, 3111
HphI GGTGA 7 cut(s) 88, 1088, 2255, 2408, 2598, 3150, 3547
Hpy166II GTNNAC 7 cut(s) 61, 96, 158, 367, 1348, 2540, 2640
Hpy8I GTNNAC 7 cut(s) 61, 96, 158, 367, 1348, 2540, 2640
HpyCH4III ACNGT 4 cut(s) 74, 1522, 3077, 3162
HpyCH4IV ACGT 2 cut(s) 3232, 3556
HpySE526I ACGT 2 cut(s) 3232, 3556
Hsp92I GRCGYC 2 cut(s) 63, 2083
Hsp92II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3155, 3338
HspAI GCGC 4 cut(s) 194, 239, 2083, 2592
KasI GGCGCC 1 cut(s) 2082
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 3594
LmnI GCTCC 5 cut(s) 628, 799, 1979, 2410, 3637
MaeII ACGT 2 cut(s) 3232, 3556
MaeIII GTNAC 8 cut(s) 517, 757, 836, 1234, 1396, 1840, 2257, 3224
MfeI CAATTG 1 cut(s) 3417
MflI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
MhlI GDGCHC 2 cut(s) 1675, 1984
MlsI TGGCCA 1 cut(s) 3296
MluNI TGGCCA 1 cut(s) 3296
Mly113I GGCGCC 1 cut(s) 2083
MlyI GAGTC 7 cut(s) 722, 868, 1097, 1288, 1493, 1763, 3224
MmeI TCCRAC 8 cut(s) 633, 1877, 1889, 1987, 2160, 2502, 3033, 3242
Mox20I TGGCCA 1 cut(s) 3296
Mph1103I ATGCAT 1 cut(s) 2443
MroNI GCCGGC 1 cut(s) 22
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 3296
MslI CAYNNNNRTG 2 cut(s) 1788, 1794
Msp20I TGGCCA 1 cut(s) 3296
MspA1I CMGCKG 4 cut(s) 153, 797, 985, 3137
MspCI CTTAAG 2 cut(s) 664, 3409
MspI CCGG 4 cut(s) 23, 124, 2003, 3111
MspR9I CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3456
MunI CAATTG 1 cut(s) 3417
Mva1269I GAATGC 2 cut(s) 2329, 3469
MvaI CCWGG 4 cut(s) 2087, 2559, 2667, 3456
MvnI CGCG 2 cut(s) 241, 3027
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 2083
NciI CCSGG 1 cut(s) 2003
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3155, 3338
NlaIV GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3633
NmeAIII GCCGAG 2 cut(s) 3598, 3608
NmuCI GTSAC 4 cut(s) 757, 836, 1234, 3224
NsiI ATGCAT 1 cut(s) 2443
NspI RCATGY 2 cut(s) 1793, 1960
NspV TTCGAA 1 cut(s) 3592
PaeI GCATGC 1 cut(s) 1793
PagI TCATGA 1 cut(s) 3334
PaqCI CACCTGC 1 cut(s) 2617
PceI AGGCCT 1 cut(s) 3578
PciSI GCTCTTC 1 cut(s) 3594
PctI GAATGC 2 cut(s) 2329, 3469
PdiI GCCGGC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 472
PfoI TCCNGGA 2 cut(s) 2665, 3454
PleI GAGTC 7 cut(s) 722, 867, 1096, 1288, 1492, 1763, 3223
PluTI GGCGCC 1 cut(s) 2086
PpsI GAGTC 7 cut(s) 722, 867, 1096, 1288, 1492, 1763, 3223
PpuMI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3452
PshBI ATTAAT 2 cut(s) 1194, 2787
Psp5II RGGWCCY 4 cut(s) 1255, 1468, 2278, 3452
Psp6I CCWGG 4 cut(s) 2085, 2557, 2665, 3454
PspFI CCCAGC 1 cut(s) 2281
PspGI CCWGG 4 cut(s) 2085, 2557, 2665, 3454
PspN4I GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3633
PspPI GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3169, 3452, 3597
PspPPI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3452
PsrI GAACNNNNNNTAC 2 cut(s) 2490, 2522
PstNI CAGNNNCTG 2 cut(s) 1937, 2672
PsuI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
PvuII CAGCTG 3 cut(s) 153, 985, 3137
RsaI GTAC 3 cut(s) 157, 2499, 3303
RsaNI GTAC 3 cut(s) 156, 2498, 3302
RseI CAYNNNNRTG 2 cut(s) 1788, 1794
SapI GCTCTTC 1 cut(s) 3594
Sau96I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3169, 3452, 3597
SchI GAGTC 7 cut(s) 722, 868, 1097, 1288, 1493, 1763, 3224
ScrFI CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3456
SduI GDGCHC 2 cut(s) 1675, 1984
SfcI CTRYAG 2 cut(s) 2334, 2901
SfoI GGCGCC 1 cut(s) 2084
SfuI TTCGAA 1 cut(s) 3592
SinI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3452
SmiMI CAYNNNNRTG 2 cut(s) 1788, 1794
SmlI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 3065, 3305, 3409, 3447
SmoI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 3065, 3305, 3409, 3447
SpeI ACTAGT 3 cut(s) 932, 3092, 3344
SphI GCATGC 1 cut(s) 1793
SseBI AGGCCT 1 cut(s) 3578
SspDI GGCGCC 1 cut(s) 2082
SspI AATATT 1 cut(s) 1579
StuI AGGCCT 1 cut(s) 3578
StyD4I CCNGG 5 cut(s) 2001, 2085, 2557, 2665, 3454
StyI CCWWGG 3 cut(s) 1014, 3297, 3579
TaaI ACNGT 4 cut(s) 74, 1522, 3077, 3162
TaiI ACGT 2 cut(s) 3235, 3559
TaqI TCGA 4 cut(s) 246, 427, 3098, 3592
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 3371
TscAI CASTG 8 cut(s) 42, 77, 412, 774, 1116, 2233, 2301, 3363
TseFI GTSAC 4 cut(s) 757, 836, 1234, 3224
Tsp45I GTSAC 4 cut(s) 757, 836, 1234, 3224
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 8 cut(s) 42, 77, 412, 774, 1116, 2233, 2301, 3363
Vha464I CTTAAG 2 cut(s) 664, 3409
VpaK11BI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3452
VspI ATTAAT 2 cut(s) 1194, 2787
XapI RAATTY 6 cut(s) 289, 570, 1371, 1888, 2034, 3622
XbaI TCTAGA 2 cut(s) 1042, 3508
XceI RCATGY 2 cut(s) 1793, 1960
XcmI CCANNNNNNNNNTGG 2 cut(s) 1429, 3149
XmnI GAANNNNTTC 1 cut(s) 472
Zsp2I ATGCAT 1 cut(s) 2443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.