MD05G1335200.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011014
Physical Location & Seq
Reverse (-)
45796219 .. 45800696
4478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1335200.v1.1.491

Sequence Viewer

Length: 3297 bp
ATGTTGGAGAAGATCGGGCTGCCGCCGAAGCCGTCACTGAGAGGGAATACTTGGGTGGTTGACGCCTCACACTGCCAGGGGTGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCATCATTGTAGGAGGTGTGGTGGCTTGTTTTGCAACAGTTGCACCCAGCAAAGAATGCTCTTACGTGGGCAAGGTGATTCGCCTGTGCGTATATGTGACCCTTGTAAAAAGCTAGAAGAAGCAGCACGCATTGAGAGATATGGACACAAGAGTAGAGCTGGGAGAGGCAGTTCAAAGTTGACATCAAATCATGAGGATGAAGTTTTGGACGAGATTCTTGGTAGTGACGGAAAGGAATTAGGACAAGAGTCAAATAGTAATGTGGTTTCCAGTATGCAGAGAGCCGCTAGTAGTGCATCATGTTCAAATGGTCAACAAAATTCCAGCCACGAGGGGGTGGGAGAAATACATAGAAGTCTTTCTGTTGATGAGCCTAATTTACAGAGTGGTGGTGGATCTGCTTCTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAGAAAAAGAAGTATAAAGTTCTTAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAGAGGGAAGGAGCTTGAGAGGCAGGCTGACGCATTGGAAATATCTTTGAGAAAAGAGCGTAGAAAGGTTTTACTCTCTGCCAATGTGGTGGAGAGCCAGATTAAAGATGGGCCTTCTGAATCTGGAAGAAGAAATAAGGTCACTCCTTCAGTGGGTAAAGAAAAAGATGATCTTTCTGCTGAGCTTAAAGAACTAGGATGGTCCGATATGGATCTCCTTGATGAAAACAAAAAGCAAGCAAGTCTGAGTTTGGAGGGTGAACTTTCTTCTCTTTTGGGAGAGGCCTCACAAAAGACCAATAAAAACAAAGGTACTAGGGCCATTGACAAAGCCCAGGTTGTTGCACTTAAAAAGAAGGCTCTCATGCTGAAGCGTGATGGGAAACTTACAGAAGCTAAGGAGGAACTGAAAAGAGCTAAAATCCTAGAGAAGGAACTCGAGGAACAGGAATTCTTGGCTGAGGCTGAAGAATCTGATGATGAAATATCTGTGTTGATTCGTGGTATGGATGATGACAAACAGGAAGAATTTTCAATTCAGTATGAGCAGGAGGATGGCTTTAATTTTGATCATCTTATTAGTGCTTCTGATGATCTGGATGGTCATTTCGAAGTGACAGATGAGGATATGGAGGACCCAGAAATAGCTGCTGCTTTACAATCTTTAGGATGGTCTCAAGATTCTAATAATGCGGAAACTTCTCCCCAGATTGCTGCCGTTGACAGGGAAGCTCTATTAAGCGAAATTCAATCATTAAAAAGAGAGGCAGTTAATCATAAACGGGCAGGTAATGTTCAAGAGGCAATGGCGCAGCTACAGAGGGCAAAACTACTTGAAAGGGACCTTGAAAGCCTTGAGTCTGAAGAGGGAAATGTTGCAAATGATCGTGCGACAATGCATAAACAAACTGCTGATAAATCGTCCAAGTCATTTATGGTGTATGATGGAAATATTAGTGCAATGGAACGTACGGACTCCAAACCTGCAAGGAAGAGTAAATTAATGATTCAGAAAGAGCTTCTGGGCTTGAAAAAGAAAGCCCTTTCTCTGAGAAGGGAAGGACGATTAAATGAGGCTGACGAAGAATTGAAGAAGGGCAGGGTTCTTGAACAGCAGCTTGAAGAGCTAGAGAATGGTTCTGTACAGAAGGCAATGCCTGGGACTGTTGGCAATAAAGTCCCAGATTTGGCACATGAGCTTCCCAATGTCTCTGAAAGTCTGCCAGTTGCAGACGAGGAAGGAGAAAATGTAACAGATCAAGATATGCATGATCCAGCATATCTTTCTATGCTAAAGAATTTGGGTTGGAATGATGACGATAATGAAGGGACCAACTCGTCGTTGGAACCTTCTAAGCAAACGGATATTCTCTCCATGAAGGTCAGTGCACCCTCTGCAACTCAAGCCCCGGCTAATGTCCCAGCTGGAGGATCAAGGAGAAGCAAAGCTGAGATTCAGAGGGAACTCTTAGGGTTGAAAAGGAAAGCTCTTGCTCTGAGGCGCCAGGGAGAGACCGAGGATGCAGAAGAACTGCTAAAAAAGGCGAAAGCACTAGAGGGCCAGATGATGGAGATGGAAGCACCCAAGGAAGTTATCACTGAACCTCCTCCTAATAGTGTTGAGGAGGAAGGAGAGGGAGGTAATGTAACAGGAAATAGTATGCAGGACCCAGCACTTTTCTCAGAGGGGACTTCCTTTTCCAAACCAGCCGTTTCTGCACCAAGAAATAAGGGTGCAATCCAAAGGGAACTTCTGGATTTAAAAAGAAAGGCTCTTGTCTTTAGACGGAAGGGAGAAACCAAAGAAGCTGAGGAAGTTTTGAGAATGGCAAAAGTGCTAGAGATTCAAATTGAAGAGATGGAAGCCCCAAAGGATTTGAGTCTGCATGATGATTCAAAGGAAGAGAAATCTGATAATTTCGGATCACTGATTAACACTGAAAAGCCAGGGAAGTTGAAGCATGATACAGATGTTAGAAGGTTTACTGAGGTGGCAATGGGTCCAATTGACGGAGTAGTTAAGTTGTCAGCAAGGAATTCTGAGTCTGTTCCTCTAACCTCACAGTTAGCTAAGGGAAACGAACCATTACCAGTGGAATTGGGTGCTTCGGGTGAAAATTATTTTCCAGACGACCAAAGAGTAGCCAAAGGCGTTAATCAGATTTCTGCGCCTGTCCAATCTGGAAACTTAGTGGATTTGTTGGCGGGGGATGACTGGAGAAGTTCTCAAAGGCCTGCTGAAAAACCTGCTGAAAAGCAAGATGATGGCCTAAAATTTGATTCTGTTGGTTCCCTTACTGCCAGTCCTCCTGTTCAGTTGGGATCCCAGACATGTTCGAATGTAAATCTGGGAAGCCAAAACAATAAATTTGATAAACAGGAAGATAAGCGAGATGTTAATGAAGCAAATTCGGTTCAGGAATCTGCTTCTCAGAGCAACCATAGTGCCATTCAGCAAGAAATCCTGGGTTTTAAGAGGAGAGCATTAGCTTTAAAGAGAGAAGGGAAACTGACCGAAGCTCGCGAGGAACTAAGGCAGGCAAAGTTATTGGAGAAGCGTCTTGATGAAGGTAGTCCTCAGTCCAAAACTACTTCAAGTGAGGCGTCCAGTGCCGCACAAAATACAGCTGGCGAGCAGAGGCAACCCCAACCATTTACAGTCACGAGACATCCCATCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006897 GO:0007034 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009057 GO:0009653 GO:0009987 GO:0010927 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030163 GO:0030435 GO:0030437 GO:0031090 GO:0031321 GO:0031410 GO:0031982 GO:0032266 GO:0032502 GO:0032505 GO:0032509 GO:0032511 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033565 GO:0034293 GO:0034613 GO:0035091 GO:0036211 GO:0036452 GO:0042763 GO:0042764 GO:0042886 GO:0043162 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043328 GO:0043412 GO:0043632 GO:0043934 GO:0043935 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045184 GO:0045324 GO:0046907 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051321 GO:0051603 GO:0051641 GO:0051649 GO:0051704 GO:0061659 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0120113 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901981 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE

Protein Analysis

1099

Amino Acids

120.85

Weight (kDa)

5.28

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 4e-17 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1951
Acc36I ACCTGC 3 cut(s) 1391, 1606, 2869
AccB1I GGYRCC 1 cut(s) 2115
AccB7I CCANNNNNTGG 1 cut(s) 2182
AccII CGCG 1 cut(s) 3138
AciI CCGC 6 cut(s) 23, 399, 593, 1307, 2819, 3228
AclWI GGATC 7 cut(s) 517, 834, 1880, 2053, 2545, 2931, 2944
AcsI RAATTY 9 cut(s) 433, 1064, 1142, 1359, 1912, 2650, 2888, 2981, 3022
AcuI CTGAAG 4 cut(s) 747, 1004, 1101, 1497
AcyI GRCGYC 3 cut(s) 63, 2116, 3218
AfaI GTAC 3 cut(s) 928, 1585, 1758
AfiI CCNNNNNNNGG 8 cut(s) 448, 767, 1045, 1338, 1801, 2042, 2182, 2624
AflIII ACRYGT 1 cut(s) 2945
AjnI CCWGG 6 cut(s) 75, 948, 1771, 2118, 2560, 3078
AjuI GAANNNNNNNTTGG 2 cut(s) 2349, 2381
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
Alw21I GWGCWC 1 cut(s) 2005
Alw26I GTCTC 4 cut(s) 1293, 1828, 2120, 3274
Alw44I GTGCAC 1 cut(s) 2001
AlwI GGATC 7 cut(s) 517, 834, 1880, 2053, 2545, 2931, 2944
Ama87I CYCGRG 1 cut(s) 1052
AoxI GGCC 6 cut(s) 725, 897, 933, 2173, 2846, 2881
ApaLI GTGCAC 1 cut(s) 2001
ApeKI GCWGC 7 cut(s) 19, 236, 1262, 1265, 1328, 1426, 1729
ApoI RAATTY 9 cut(s) 433, 1064, 1142, 1359, 1912, 2650, 2888, 2981, 3022
AseI ATTAAT 1 cut(s) 1616
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 3 cut(s) 1426, 2118, 2785
AspS9I GGNCC 9 cut(s) 725, 816, 933, 1249, 1456, 1944, 2173, 2281, 2615
AsuC2I CCSGG 1 cut(s) 2024
AsuHPI GGTGA 4 cut(s) 88, 200, 884, 2738
AsuII TTCGAA 2 cut(s) 1224, 2951
AvaI CYCGRG 1 cut(s) 1052
AvaII GGWCC 6 cut(s) 816, 1249, 1456, 1944, 2281, 2615
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 2005
BaeI ACNNNNGTAYC 2 cut(s) 2571, 2604
BamHI GGATCC 1 cut(s) 2936
BanI GGYRCC 1 cut(s) 2115
BauI CACGAG 2 cut(s) 443, 3277
Bbv12I GWGCWC 1 cut(s) 2005
BbvCI CCTCAGC 2 cut(s) 1074, 2424
BbvI GCAGC 7 cut(s) 6, 248, 1249, 1252, 1315, 1438, 1741
BceAI ACGGC 3 cut(s) 16, 1316, 2309
BciT130I CCWGG 6 cut(s) 77, 950, 1773, 2120, 2562, 3080
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 1 cut(s) 2024
BcoDI GTCTC 4 cut(s) 1293, 1828, 2120, 3274
BfaI CTAG 9 cut(s) 227, 402, 809, 930, 1040, 1742, 2168, 2453, 3295
BfmI CTRYAG 1 cut(s) 1430
BfoI RGCGCY 1 cut(s) 2119
BfuAI ACCTGC 3 cut(s) 1391, 1606, 2869
BlpI GCTNAGC 1 cut(s) 795
Bme1390I CCNGG 7 cut(s) 77, 950, 1773, 2024, 2120, 2562, 3080
Bme18I GGWCC 6 cut(s) 816, 1249, 1456, 1944, 2281, 2615
BmeT110I CYCGRG 1 cut(s) 1052
BmgT120I GGNCC 9 cut(s) 725, 816, 933, 1249, 1456, 1944, 2173, 2281, 2615
BmiI GGNNCC 9 cut(s) 1251, 1457, 1945, 1962, 2117, 2283, 2616, 2905, 2938
BmrFI CCNGG 7 cut(s) 77, 950, 1773, 2024, 2120, 2562, 3080
BmsI GCATC 3 cut(s) 124, 419, 2125
BoxI GACNNNNGTC 1 cut(s) 361
BplI GAGNNNNNCTC 2 cut(s) 2824, 2856
BpmI CTGGAG 2 cut(s) 2061, 2851
Bpu10I CCTNAGC 4 cut(s) 1011, 1074, 2424, 2685
Bpu1102I GCTNAGC 1 cut(s) 795
Bpu14I TTCGAA 2 cut(s) 1224, 2951
BpuEI CTTGAG 4 cut(s) 650, 1275, 1490, 2001
BpuMI CCSGG 1 cut(s) 2024
BsaAI YACGTR 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 825
BsaHI GRCGYC 3 cut(s) 63, 2116, 3218
BsaI GGTCTC 2 cut(s) 1293, 2120
BsaJI CCNNGG 9 cut(s) 76, 948, 1772, 2022, 2119, 2130, 2199, 2561, 3079
Bsc4I CCNNNNNNNGG 8 cut(s) 448, 767, 1045, 1338, 1801, 2042, 2182, 2624
Bse1I ACTGG 6 cut(s) 384, 1838, 2705, 2834, 2916, 3222
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 4 cut(s) 1425, 1581, 1773, 2616
Bse8I GATNNNNATC 1 cut(s) 825
BseBI CCWGG 6 cut(s) 77, 950, 1773, 2120, 2562, 3080
BseDI CCNNGG 9 cut(s) 76, 948, 1772, 2022, 2119, 2130, 2199, 2561, 3079
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 8 cut(s) 448, 767, 1045, 1338, 1801, 2042, 2182, 2624
BseMI GCAATG 4 cut(s) 1425, 1581, 1773, 2616
BseNI ACTGG 6 cut(s) 384, 1838, 2705, 2834, 2916, 3222
BseRI GAGGAG 3 cut(s) 2211, 2252, 3106
BseSI GKGCMC 1 cut(s) 2005
BseXI GCAGC 7 cut(s) 6, 248, 1249, 1252, 1315, 1438, 1741
BseYI CCCAGC 4 cut(s) 159, 272, 2035, 2284
BsgI GTGCAG 1 cut(s) 2316
Bsh1236I CGCG 1 cut(s) 3138
BshFI GGCC 6 cut(s) 727, 899, 935, 2175, 2848, 2883
BshNI GGYRCC 1 cut(s) 2115
BsiHKAI GWGCWC 1 cut(s) 2005
BsiHKCI CYCGRG 1 cut(s) 1052
BsiSI CCGG 1 cut(s) 2024
BsiWI CGTACG 1 cut(s) 1583
BslFI GGGAC 6 cut(s) 1469, 1778, 1789, 1957, 2018, 2317
BslI CCNNNNNNNGG 8 cut(s) 448, 767, 1045, 1338, 1801, 2042, 2182, 2624
BsmAI GTCTC 4 cut(s) 1293, 1828, 2120, 3274
BsmFI GGGAC 6 cut(s) 1469, 1778, 1789, 1957, 2018, 2317
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 6 cut(s) 727, 899, 935, 2175, 2848, 2883
Bso31I GGTCTC 2 cut(s) 1293, 2120
BsoBI CYCGRG 1 cut(s) 1052
Bsp119I TTCGAA 2 cut(s) 1224, 2951
Bsp1286I GDGCHC 1 cut(s) 2005
Bsp1407I TGTACA 1 cut(s) 1756
Bsp1720I GCTNAGC 1 cut(s) 795
Bsp68I TCGCGA 1 cut(s) 3138
BspACI CCGC 6 cut(s) 23, 399, 593, 1307, 2819, 3228
BspANI GGCC 6 cut(s) 727, 899, 935, 2175, 2848, 2883
BspFNI CGCG 1 cut(s) 3138
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 9 cut(s) 1251, 1457, 1945, 1962, 2117, 2283, 2616, 2905, 2938
BspMI ACCTGC 3 cut(s) 1391, 1606, 2869
BspPI GGATC 7 cut(s) 517, 834, 1880, 2053, 2545, 2931, 2944
BspQI GCTCTTC 1 cut(s) 1731
BspT104I TTCGAA 2 cut(s) 1224, 2951
BspT107I GGYRCC 1 cut(s) 2115
BspTNI GGTCTC 2 cut(s) 1293, 2120
BsrDI GCAATG 4 cut(s) 1425, 1581, 1773, 2616
BsrGI TGTACA 1 cut(s) 1756
BsrI ACTGG 6 cut(s) 384, 1838, 2705, 2834, 2916, 3222
BssECI CCNNGG 9 cut(s) 76, 948, 1772, 2022, 2119, 2130, 2199, 2561, 3079
BssNI GRCGYC 3 cut(s) 63, 2116, 3218
BssSI CACGAG 2 cut(s) 443, 3277
BssT1I CCWWGG 1 cut(s) 2199
Bst2BI CACGAG 2 cut(s) 443, 3277
Bst2UI CCWGG 6 cut(s) 77, 950, 1773, 2120, 2562, 3080
Bst4CI ACNGT 4 cut(s) 152, 1780, 2679, 3274
Bst6I CTCTTC 5 cut(s) 1473, 1601, 1731, 2463, 2511
BstACI GRCGYC 3 cut(s) 63, 2116, 3218
BstAPI GCANNNNNTGC 3 cut(s) 153, 169, 2009
BstAUI TGTACA 1 cut(s) 1756
BstBAI YACGTR 1 cut(s) 179
BstBI TTCGAA 2 cut(s) 1224, 2951
BstENI CCTNNNNNAGG 1 cut(s) 1043
BstFNI CGCG 1 cut(s) 3138
BstH2I RGCGCY 1 cut(s) 2119
BstHHI GCGC 3 cut(s) 1426, 2118, 2785
BstMAI GTCTC 4 cut(s) 1293, 1828, 2120, 3274
BstNI CCWGG 6 cut(s) 77, 950, 1773, 2120, 2562, 3080
BstNSI RCATGY 1 cut(s) 2949
BstPAI GACNNNNGTC 1 cut(s) 361
BstSCI CCNGG 7 cut(s) 75, 948, 1771, 2022, 2118, 2560, 3078
BstSFI CTRYAG 1 cut(s) 1430
BstSLI GKGCMC 1 cut(s) 2005
BstUI CGCG 1 cut(s) 3138
BstV1I GCAGC 7 cut(s) 6, 248, 1249, 1252, 1315, 1438, 1741
BstX2I RGATCY 3 cut(s) 509, 826, 2936
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 3 cut(s) 509, 826, 2936
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 6 cut(s) 727, 899, 935, 2175, 2848, 2883
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 9 cut(s) 35, 70, 771, 2005, 2211, 2540, 2550, 2712, 3229
BtuMI TCGCGA 1 cut(s) 3138
BveI ACCTGC 3 cut(s) 1391, 1606, 2869
CciI TCATGA 1 cut(s) 304
CfoI GCGC 3 cut(s) 1426, 2118, 2785
Cfr13I GGNCC 9 cut(s) 725, 816, 933, 1249, 1456, 1944, 2173, 2281, 2615
CseI GACGC 5 cut(s) 71, 521, 653, 3161, 3207
Csp6I GTAC 3 cut(s) 927, 1584, 1757
CviAII CATG 9 cut(s) 305, 414, 979, 1808, 1883, 1990, 2501, 2576, 2946
CviQI GTAC 3 cut(s) 927, 1584, 1757
DinI GGCGCC 1 cut(s) 2117
DraI TTTAAA 2 cut(s) 2376, 3108
DrdI GACNNNNNNGTC 1 cut(s) 1951
DseDI GACNNNNNNGTC 1 cut(s) 1951
Eam1104I CTCTTC 5 cut(s) 1473, 1601, 1731, 2463, 2511
EarI CTCTTC 5 cut(s) 1473, 1601, 1731, 2463, 2511
Eco130I CCWWGG 1 cut(s) 2199
Eco147I AGGCCT 2 cut(s) 899, 2848
Eco31I GGTCTC 2 cut(s) 1293, 2120
Eco47I GGWCC 6 cut(s) 816, 1249, 1456, 1944, 2281, 2615
Eco57I CTGAAG 4 cut(s) 747, 1004, 1101, 1497
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 1052
EcoNI CCTNNNNNAGG 1 cut(s) 1043
EcoO109I RGGNCCY 3 cut(s) 1249, 1456, 2281
EcoRI GAATTC 2 cut(s) 1064, 2650
EcoRII CCWGG 6 cut(s) 75, 948, 1771, 2118, 2560, 3078
EcoT14I CCWWGG 1 cut(s) 2199
EcoT22I ATGCAT 2 cut(s) 1515, 1884
EgeI GGCGCC 1 cut(s) 2117
EheI GGCGCC 1 cut(s) 2117
ErhI CCWWGG 1 cut(s) 2199
FaeI CATG 9 cut(s) 308, 417, 982, 1811, 1886, 1993, 2504, 2579, 2949
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 6 cut(s) 1469, 1778, 1789, 1957, 2018, 2317
FatI CATG 9 cut(s) 304, 413, 978, 1807, 1882, 1989, 2500, 2575, 2945
FauI CCCGC 1 cut(s) 2812
FbaI TGATCA 1 cut(s) 1183
FspBI CTAG 9 cut(s) 227, 402, 809, 930, 1040, 1742, 2168, 2453, 3295
GlaI GCGC 3 cut(s) 1425, 2117, 2784
GsaI CCCAGC 4 cut(s) 163, 276, 2039, 2288
GsuI CTGGAG 2 cut(s) 2061, 2851
HaeII RGCGCY 1 cut(s) 2119
HaeIII GGCC 6 cut(s) 727, 899, 935, 2175, 2848, 2883
HapII CCGG 1 cut(s) 2024
HgaI GACGC 5 cut(s) 71, 521, 653, 3161, 3207
HhaI GCGC 3 cut(s) 1426, 2118, 2785
Hin1I GRCGYC 3 cut(s) 63, 2116, 3218
Hin1II CATG 9 cut(s) 308, 417, 982, 1811, 1886, 1993, 2504, 2579, 2949
Hin6I GCGC 3 cut(s) 1424, 2116, 2783
HinP1I GCGC 3 cut(s) 1424, 2116, 2783
HincII GTYRAC 4 cut(s) 61, 294, 428, 1336
HindII GTYRAC 4 cut(s) 61, 294, 428, 1336
HindIII AAGCTT 1 cut(s) 540
HpaII CCGG 1 cut(s) 2024
HphI GGTGA 4 cut(s) 88, 200, 884, 2738
Hpy166II GTNNAC 8 cut(s) 61, 96, 294, 428, 875, 1336, 2003, 2598
Hpy8I GTNNAC 8 cut(s) 61, 96, 294, 428, 875, 1336, 2003, 2598
Hpy99I CGWCG 1 cut(s) 1957
HpyCH4III ACNGT 4 cut(s) 152, 1780, 2679, 3274
HpyCH4IV ACGT 2 cut(s) 178, 1582
HpySE526I ACGT 2 cut(s) 178, 1582
Hsp92I GRCGYC 3 cut(s) 63, 2116, 3218
Hsp92II CATG 9 cut(s) 308, 417, 982, 1811, 1886, 1993, 2504, 2579, 2949
HspAI GCGC 3 cut(s) 1424, 2116, 2783
KasI GGCGCC 1 cut(s) 2115
Ksp22I TGATCA 1 cut(s) 1183
LguI GCTCTTC 1 cut(s) 1731
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 7 cut(s) 6, 248, 1249, 1252, 1315, 1438, 1741
LweI GCATC 3 cut(s) 124, 419, 2125
MaeI CTAG 9 cut(s) 227, 402, 809, 930, 1040, 1742, 2168, 2453, 3295
MaeII ACGT 2 cut(s) 178, 1582
MaeIII GTNAC 8 cut(s) 33, 209, 338, 754, 1228, 1864, 2260, 3274
MfeI CAATTG 1 cut(s) 2619
MflI RGATCY 3 cut(s) 509, 826, 2936
MhlI GDGCHC 1 cut(s) 2005
Mly113I GGCGCC 1 cut(s) 2116
MlyI GAGTC 5 cut(s) 371, 1481, 1583, 2503, 2666
MmeI TCCRAC 2 cut(s) 1901, 1938
Mph1103I ATGCAT 2 cut(s) 1515, 1884
MroXI GAANNNNTTC 1 cut(s) 472
MslI CAYNNNNRTG 1 cut(s) 309
MspA1I CMGCKG 2 cut(s) 2039, 3242
MspI CCGG 1 cut(s) 2024
MspR9I CCNGG 7 cut(s) 77, 950, 1773, 2024, 2120, 2562, 3080
MunI CAATTG 1 cut(s) 2619
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 6 cut(s) 77, 950, 1773, 2120, 2562, 3080
MvnI CGCG 1 cut(s) 3138
NarI GGCGCC 1 cut(s) 2116
NciI CCSGG 1 cut(s) 2024
NlaIII CATG 9 cut(s) 308, 417, 982, 1811, 1886, 1993, 2504, 2579, 2949
NlaIV GGNNCC 9 cut(s) 1251, 1457, 1945, 1962, 2117, 2283, 2616, 2905, 2938
NmuCI GTSAC 6 cut(s) 33, 209, 338, 754, 1228, 3274
NruI TCGCGA 1 cut(s) 3138
NsiI ATGCAT 2 cut(s) 1515, 1884
NspI RCATGY 1 cut(s) 2949
NspV TTCGAA 2 cut(s) 1224, 2951
PaeR7I CTCGAG 1 cut(s) 1052
PagI TCATGA 1 cut(s) 304
PceI AGGCCT 2 cut(s) 899, 2848
PciI ACATGT 1 cut(s) 2945
PciSI GCTCTTC 1 cut(s) 1731
PctI GAATGC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 472
Pfl23II CGTACG 1 cut(s) 1583
PflMI CCANNNNNTGG 1 cut(s) 2182
PleI GAGTC 5 cut(s) 370, 1480, 1583, 2502, 2665
PluTI GGCGCC 1 cut(s) 2119
PpsI GAGTC 5 cut(s) 370, 1480, 1583, 2502, 2665
Ppu21I YACGTR 1 cut(s) 179
PpuMI RGGWCCY 3 cut(s) 1249, 1456, 2281
PscI ACATGT 1 cut(s) 2945
PshAI GACNNNNGTC 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 1616
Psp5II RGGWCCY 3 cut(s) 1249, 1456, 2281
Psp6I CCWGG 6 cut(s) 75, 948, 1771, 2118, 2560, 3078
PspFI CCCAGC 4 cut(s) 159, 272, 2035, 2284
PspGI CCWGG 6 cut(s) 75, 948, 1771, 2118, 2560, 3078
PspLI CGTACG 1 cut(s) 1583
PspN4I GGNNCC 9 cut(s) 1251, 1457, 1945, 1962, 2117, 2283, 2616, 2905, 2938
PspPI GGNCC 9 cut(s) 725, 816, 933, 1249, 1456, 1944, 2173, 2281, 2615
PspPPI RGGWCCY 3 cut(s) 1249, 1456, 2281
PspXI VCTCGAGB 1 cut(s) 1052
PsuI RGATCY 3 cut(s) 509, 826, 2936
PvuII CAGCTG 2 cut(s) 2039, 3242
RruI TCGCGA 1 cut(s) 3138
RsaI GTAC 3 cut(s) 928, 1585, 1758
RsaNI GTAC 3 cut(s) 927, 1584, 1757
RseI CAYNNNNRTG 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 1731
Sau96I GGNCC 9 cut(s) 725, 816, 933, 1249, 1456, 1944, 2173, 2281, 2615
SchI GAGTC 5 cut(s) 371, 1481, 1583, 2503, 2666
ScrFI CCNGG 7 cut(s) 77, 950, 1773, 2024, 2120, 2562, 3080
SduI GDGCHC 1 cut(s) 2005
SfaNI GCATC 3 cut(s) 124, 419, 2125
SfcI CTRYAG 1 cut(s) 1430
SfoI GGCGCC 1 cut(s) 2117
Sfr274I CTCGAG 1 cut(s) 1052
SfuI TTCGAA 2 cut(s) 1224, 2951
SinI GGWCC 6 cut(s) 816, 1249, 1456, 1944, 2281, 2615
SlaI CTCGAG 1 cut(s) 1052
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 5 cut(s) 629, 1052, 1290, 1469, 2016
SmoI CTYRAG 5 cut(s) 629, 1052, 1290, 1469, 2016
SseBI AGGCCT 2 cut(s) 899, 2848
SsiI CCGC 6 cut(s) 23, 399, 593, 1307, 2819, 3228
SspDI GGCGCC 1 cut(s) 2115
SspI AATATT 1 cut(s) 1567
SspMI CTAG 9 cut(s) 227, 402, 809, 930, 1040, 1742, 2168, 2453, 3295
StuI AGGCCT 2 cut(s) 899, 2848
StyD4I CCNGG 7 cut(s) 75, 948, 1771, 2022, 2118, 2560, 3078
StyI CCWWGG 1 cut(s) 2199
TaaI ACNGT 4 cut(s) 152, 1780, 2679, 3274
TaiI ACGT 2 cut(s) 181, 1585
TaqI TCGA 3 cut(s) 1053, 1224, 2951
TaqII GACCGA 2 cut(s) 2144, 3143
TatI WGTACW 1 cut(s) 1756
TauI GCSGC 3 cut(s) 25, 401, 3230
TscAI CASTG 9 cut(s) 42, 77, 771, 2005, 2218, 2547, 2557, 2712, 3229
TseFI GTSAC 6 cut(s) 33, 209, 338, 754, 1228, 3274
TseI GCWGC 7 cut(s) 19, 236, 1262, 1265, 1328, 1426, 1729
Tsp45I GTSAC 6 cut(s) 33, 209, 338, 754, 1228, 3274
TspDTI ATGAA 8 cut(s) 91, 327, 852, 1110, 1953, 2006, 3030, 3195
TspGWI ACGGA 5 cut(s) 357, 1601, 1991, 2416, 2640
TspRI CASTG 9 cut(s) 42, 77, 771, 2005, 2218, 2547, 2557, 2712, 3229
Van91I CCANNNNNTGG 1 cut(s) 2182
VneI GTGCAC 1 cut(s) 2001
VpaK11BI GGWCC 6 cut(s) 816, 1249, 1456, 1944, 2281, 2615
VspI ATTAAT 1 cut(s) 1616
XagI CCTNNNNNAGG 1 cut(s) 1043
XapI RAATTY 9 cut(s) 433, 1064, 1142, 1359, 1912, 2650, 2888, 2981, 3022
XceI RCATGY 1 cut(s) 2949
XcmI CCANNNNNNNNNTGG 3 cut(s) 719, 1546, 1954
XhoI CTCGAG 1 cut(s) 1052
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 9 cut(s) 227, 402, 809, 930, 1040, 1742, 2168, 2453, 3295
Zsp2I ATGCAT 2 cut(s) 1515, 1884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.