Rh5BG040800

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011014
Physical Location & Seq
Forward (+)
2974733 .. 2979578
4846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG040800.1

Sequence Viewer

Length: 3591 bp
ATGCTGGAGAAGATCGGGTTGCCGGCGAAGCCATCGCTGAGAGGGAATAATTGGGTGGTTGACGCCTCACACTGTCAGGGATGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCATCATTGCCGGAGGTGTGGGGGCATTTTCTGTGGCAGCTGTACACAGCAGAGGATGTTTTTGCGTGGGCAAGGCGATGCGCCTGTGCGGATATGTGAGCCTTGTAAGAAGCTAGAAGAAGCAGCGCGGTTCGAGAGATATGGACACAAGAGTAGAGCTGCGAGAGGCAGTTCAAATTTGACATCAAAGCCTGGGGATGAAATTTTGAACCAGATACTTGGTAATGAAGGGAAGGAATCAGGACAAGAGGTTAACAAGAATGTAGTTTCCAGTATGCTGAGGGCAACTAGTAGTGCATCATGTTCAAATAGTCGAGAAGATTCCAGCCATGATGCAGTGGGAGAAATACTTAGAAGTGTTTCTGTTGATAAGTTTAGTCAATTACAGAATGATAGTGAATCTGCTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAAAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCGTTGGAAATATCCTTAAGAAAAAAGCGTAAAAAGGATTTACTCTCTGACAATGTGGCTGAGGGCCAGATTAAAGATGACTCTTCCCAATCTGGAAGAAGAAACAGGGTCACTACTCCAGTGGGTAAAGAAAAAGATGACCTTTCAGCGGAGCTTAAAGAATTAGGGTGGTCTGATATGGATCTCCGTGACGAGAATAAAAAACAAGCGAGTCTGACTTTGGAGGGCGAGCTCTCTTCTCTTCTGGGAGAAATCTCACAAAAGAGCAATAAAAGTAAGGCTACTAGTGCCATTGACAAAACACAAGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGCAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTCGAAGAACAGGAATTGTTGGGTGAGGCTGAAGAGTCTGATGATGAAATATCTGCACTGATTCGTAGTATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAGGATGGCCTTAATTTTGATAGTCTTATTAATGCTGCTGATGATCATATTATAGATAGTAATTTTGAAGTGACTGACGATGATATGGAGGACCCAGAAATAACTGCTGCCTTAAAATCTTTAGGATGGACTCAAGATTCAAATAATCCTCCTGAAACCTCTGCTACCCAGATAGCTTCAGTTGACAAGGAAGCACTATTAAGTGAAATTCAATTCTTAAAAAGAGAGGCTGTAACTCTTAAACGAGCAGGTAATGCCACAGAAGCAATGGCGCAGCTAAAGAAGGCAAAACGGCTTGAAAGGGACCTTGAAAGCATCGAGTCCCAAGAGGGCAATGTCGTGAAACCGTCTGCAACAGTTCATAATCGAACTATTGATAGATCATCAAAGTCATCTATGGTGGATGATGGAAATATTAGTGCAATGGAACATATTGACTTCAAACCTGCGAGGAAGAGTAAGTTAATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAGGGACGATTAGATGAAGCTGAAGAAGAGTTGAAGAGAGGCAGGGTTCTTGAGCATCAGCTTGAAGAGATGGAGAATGGTAAGGACTCAGTTTTGGAACATGAGCATGCCAATGTCTCTGGAAATCTGTCTGTTGCGGATGAGGAAGGAGAGAATGTAACAGATCAAGATATGTATGATCCAACATATCTTTCCATGCTAAGGAATTTGGGTTGGAATGATGATAATGAAGTTGCAAACTCTTCATCAGCACCTGATAAGAAAACTGATAACATGCAGGCTAGTGAATCTTCTGTAGCACAAGCCCTAAGTGATTTCCCGGTTGGAGGATCAAGGAAAGGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAGGCTCTTGCTCTGAGGCGCCAGGGAGACACCGAGGAAGCAGAAGAAATGCTGAAAAAGGCAAAAGCATTAGAGGGTGAGATGGCGGAGATGGAAGCACCGAAGAAAGTTCAGTCGGACTTTGTTAAGGACAAGGACAATTTCATTGATCCTCTTCCTCTCAGTGTAGAGGAAGAAGGTGATGGCGGGGATGTAACAGAAAATGATGTGCAGGACCCAGCACTACTCTCAGTGCTAAAGAGTTTGGGGTGGGAGGATAAAGAGCATTCTGTAAAGGCATCTCCTCTGCAATCTTCTTCCAAACTTGCTGTTACTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTTTGGATTTGAAAAGAAAGGCTCATGCGTTTAGACGAAAGGGACAGACTGAAGAAGCTGAAGAAGTGTTGAAGATGGCAAAGGTACTAGAAGTTCAAATTGAAGAGTTGGAAGCCCCAAAGGGTTTACCAATGGAATTGGGTCCAGGTCGTCCAAACAACCAGAGAATTGCTCAAGGCGCTAGTCAGTCTTCACCTGCCCAATCTGGGAACTTTGCAGATTTGTTGACGGGGGATGACTGGAGTGGTTCTCCAGGATCTGCTGAAAAACAATATGATAACCTATCTTGTTCTGTTGATTCTGTTAATGCCAGTCCTCCCATTCAGTTGAGATCCTCACAGGAAGATCTGAGAAAAAGAGATGATGCAATAATTAATAAACGGGAAGATACGATGGTAGCCAATGAGAAGCAAGATGCTAAAGAGGCAAATTTGGTTCTGGGATCTTCTCCACAGAAGAATGAAAGTGCCATTCGGCAAGAAATCGTGGCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGAAAACTGACAGAAGCCCGCGAAGAACTTAGGCAGGCAAAAGTGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTCGAGTGATGTGTCCGGTTCTGCTAACAATGTTCCTCCAGCTGCCCAAAAGCAGCATGGTTCACCGTCCTCGGGCCAGAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGATTGAAGAAGCGGAAGCAGAGTTTGAATTGGCCAAGGCACTTGAGGCTCAGTTAGAGGAAAGTGCCGCTCATGATTCCACAACTGTAGCACCAGTGGATGATGCGGCCGTAGAGGGACTTCTTGATCCTGAACTTCTGTCCGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACTACATCATCTCAAGGTCCTGGAAGACCAGAGCATTCAAAACCCAATGTTGGCAAGAGTGACAACGTAATCCAAGATAGAAGTACCCTGGAGGAACAGATCAAGGCAGAGAAGGTGAAGGCACTAAATCTGAAACGTGCAGGAAAACAAGCCGAGGCCTTGGATGCTCTTCGAAAGGCCAAGATGCTTGAAAAGAAGCTGAATTCCTCGGCTCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1196

Amino Acids

131.94

Weight (kDa)

5.45

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 1.6e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2617
Acc36I ACCTGC 3 cut(s) 1403, 1618, 2617
AccB1I GGYRCC 1 cut(s) 2082
AccBSI CCGCTC 1 cut(s) 3263
AccII CGCG 2 cut(s) 241, 2958
AclWI GGATC 8 cut(s) 837, 1856, 2020, 2207, 2677, 2739, 2863, 3314
AcoI YGGCCR 2 cut(s) 3225, 3300
AcsI RAATTY 8 cut(s) 289, 315, 570, 1371, 1888, 2034, 2842, 3571
AcuI CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
AcyI GRCGYC 2 cut(s) 63, 2083
AfaI GTAC 3 cut(s) 157, 2499, 3454
AfiI CCNNNNNNNGG 6 cut(s) 796, 1884, 2009, 2619, 3098, 3419
AflII CTTAAG 2 cut(s) 664, 3340
AhlI ACTAGT 3 cut(s) 401, 932, 3023
AjnI CCWGG 6 cut(s) 304, 2085, 2557, 2665, 3388, 3456
AjuI GAANNNNNNNTTGG 2 cut(s) 1858, 1890
Alw21I GWGCWC 2 cut(s) 882, 1675
Alw26I GTCTC 4 cut(s) 1672, 1804, 2085, 3153
AlwI GGATC 8 cut(s) 837, 1856, 2020, 2207, 2677, 2739, 2863, 3314
AlwNI CAGNNNCTG 2 cut(s) 1937, 2672
Ama87I CYCGRG 1 cut(s) 3097
AoxI GGCC 8 cut(s) 712, 1171, 1665, 3100, 3225, 3300, 3525, 3546
ApeKI GCWGC 9 cut(s) 150, 236, 272, 1199, 1271, 1438, 3068, 3079, 3178
ApoI RAATTY 8 cut(s) 289, 315, 570, 1371, 1888, 2034, 2842, 3571
ArsI GACNNNNNNTTYG 2 cut(s) 3175, 3207
AseI ATTAAT 2 cut(s) 1194, 2787
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 5 cut(s) 196, 241, 1438, 2085, 2594
AspS9I GGNCC 8 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3386
AsuC2I CCSGG 1 cut(s) 2003
AsuHPI GGTGA 8 cut(s) 88, 1088, 2153, 2255, 2408, 2598, 3081, 3496
AsuII TTCGAA 1 cut(s) 3541
AvaI CYCGRG 1 cut(s) 3097
AvaII GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3386
AxyI CCTNAGG 1 cut(s) 525
BaeI ACNNNNGTAYC 2 cut(s) 2793, 2826
BalI TGGCCA 1 cut(s) 3227
BanI GGYRCC 1 cut(s) 2082
BanII GRGCYC 1 cut(s) 882
BarI GAAGNNNNNNTAC 2 cut(s) 3297, 3329
BbsI GAAGAC 3 cut(s) 2595, 3009, 3400
Bbv12I GWGCWC 2 cut(s) 882, 1675
BbvCI CCTCAGC 2 cut(s) 392, 708
BbvI GCAGC 9 cut(s) 162, 248, 259, 1186, 1258, 1450, 3055, 3091, 3165
BceAI ACGGC 2 cut(s) 1472, 3287
BciT130I CCWGG 6 cut(s) 306, 2087, 2559, 2667, 3390, 3458
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 2003
BcoDI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BcuI ACTAGT 3 cut(s) 401, 932, 3023
BfaI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
BfmI CTRYAG 2 cut(s) 1977, 3279
BfoI RGCGCY 2 cut(s) 2086, 2595
BfrI CTTAAG 2 cut(s) 664, 3340
BfuAI ACCTGC 3 cut(s) 1403, 1618, 2617
BglII AGATCT 1 cut(s) 2758
Bme1390I CCNGG 7 cut(s) 306, 2003, 2087, 2559, 2667, 3390, 3458
Bme18I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3386
BmeT110I CYCGRG 1 cut(s) 3097
BmgT120I GGNCC 8 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3386
BmiI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3582
BmrFI CCNGG 7 cut(s) 306, 2003, 2087, 2559, 2667, 3390, 3458
BpiI GAAGAC 3 cut(s) 2595, 3009, 3400
BplI GAGNNNNNCTC 4 cut(s) 2569, 2601, 2647, 2679
BpmI CTGGAG 7 cut(s) 26, 750, 965, 2649, 2674, 3048, 3479
Bpu10I CCTNAGC 3 cut(s) 392, 708, 1883
Bpu14I TTCGAA 1 cut(s) 3541
BpuEI CTTGAG 7 cut(s) 653, 1281, 1754, 2571, 3017, 3257, 3366
BpuMI CCSGG 1 cut(s) 2003
BsaBI GATNNNNATC 1 cut(s) 828
BsaHI GRCGYC 2 cut(s) 63, 2083
BsaWI WCCGGW 1 cut(s) 3041
BsaXI ACNNNNNCTCC 2 cut(s) 1824, 1854
Bsc4I CCNNNNNNNGG 6 cut(s) 796, 1884, 2009, 2619, 3098, 3419
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
Bse21I CCTNAGG 1 cut(s) 525
Bse3DI GCAATG 4 cut(s) 118, 1437, 1504, 1593
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 6 cut(s) 306, 2087, 2559, 2667, 3390, 3458
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 6 cut(s) 796, 1884, 2009, 2619, 3098, 3419
BseMI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BseNI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BseRI GAGGAG 2 cut(s) 2337, 2926
BseX3I CGGCCG 1 cut(s) 3300
BseXI GCAGC 9 cut(s) 162, 248, 259, 1186, 1258, 1450, 3055, 3091, 3165
BseYI CCCAGC 1 cut(s) 2281
BsgI GTGCAG 4 cut(s) 1092, 2294, 2364, 3528
Bsh1236I CGCG 2 cut(s) 241, 2958
Bsh1285I CGRYCG 1 cut(s) 3303
BshFI GGCC 8 cut(s) 714, 1173, 1667, 3102, 3227, 3302, 3527, 3548
BshNI GGYRCC 1 cut(s) 2082
BsiEI CGRYCG 1 cut(s) 3303
BsiHKAI GWGCWC 2 cut(s) 882, 1675
BsiHKCI CYCGRG 1 cut(s) 3097
BsiSI CCGG 4 cut(s) 23, 124, 2003, 3042
BslFI GGGAC 5 cut(s) 1471, 1481, 1701, 2469, 3324
BslI CCNNNNNNNGG 6 cut(s) 796, 1884, 2009, 2619, 3098, 3419
BsmAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BsmFI GGGAC 5 cut(s) 1471, 1481, 1701, 2469, 3324
BsmI GAATGC 2 cut(s) 2329, 3403
BsnI GGCC 8 cut(s) 714, 1173, 1667, 3102, 3227, 3302, 3527, 3548
BsoBI CYCGRG 1 cut(s) 3097
Bsp119I TTCGAA 1 cut(s) 3541
Bsp1286I GDGCHC 2 cut(s) 882, 1675
Bsp1407I TGTACA 1 cut(s) 155
BspANI GGCC 8 cut(s) 714, 1173, 1667, 3102, 3227, 3302, 3527, 3548
BspFNI CGCG 2 cut(s) 241, 2958
BspHI TCATGA 1 cut(s) 3265
BspLI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3582
BspMI ACCTGC 3 cut(s) 1403, 1618, 2617
BspPI GGATC 8 cut(s) 837, 1856, 2020, 2207, 2677, 2739, 2863, 3314
BspQI GCTCTTC 1 cut(s) 3543
BspT104I TTCGAA 1 cut(s) 3541
BspT107I GGYRCC 1 cut(s) 2082
BspTI CTTAAG 2 cut(s) 664, 3340
BsrBI CCGCTC 1 cut(s) 3263
BsrDI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BsrFI RCCGGY 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 155
BsrI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BssAI RCCGGY 1 cut(s) 22
BssNI GRCGYC 2 cut(s) 63, 2083
BssT1I CCWWGG 3 cut(s) 1014, 3228, 3528
Bst2UI CCWGG 6 cut(s) 306, 2087, 2559, 2667, 3390, 3458
Bst4CI ACNGT 6 cut(s) 74, 1512, 1522, 3008, 3093, 3280
BstACI GRCGYC 2 cut(s) 63, 2083
BstAFI CTTAAG 2 cut(s) 664, 3340
BstAPI GCANNNNNTGC 1 cut(s) 1418
BstAUI TGTACA 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 3541
BstFNI CGCG 2 cut(s) 241, 2958
BstH2I RGCGCY 2 cut(s) 2086, 2595
BstHHI GCGC 5 cut(s) 196, 241, 1438, 2085, 2594
BstMAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BstMCI CGRYCG 1 cut(s) 3303
BstNI CCWGG 6 cut(s) 306, 2087, 2559, 2667, 3390, 3458
BstNSI RCATGY 2 cut(s) 1793, 1960
BstSCI CCNGG 7 cut(s) 304, 2001, 2085, 2557, 2665, 3388, 3456
BstSFI CTRYAG 2 cut(s) 1977, 3279
BstUI CGCG 2 cut(s) 241, 2958
BstV1I GCAGC 9 cut(s) 162, 248, 259, 1186, 1258, 1450, 3055, 3091, 3165
BstV2I GAAGAC 3 cut(s) 2595, 3009, 3400
BstX2I RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstZI CGGCCG 1 cut(s) 3300
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 8 cut(s) 714, 1173, 1667, 3102, 3227, 3302, 3527, 3548
BtgZI GCGATG 2 cut(s) 18, 204
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 7 cut(s) 70, 456, 774, 1109, 2233, 2301, 3294
BveI ACCTGC 3 cut(s) 1403, 1618, 2617
CaiI CAGNNNCTG 2 cut(s) 1937, 2672
CciI TCATGA 1 cut(s) 3265
CfoI GCGC 5 cut(s) 196, 241, 1438, 2085, 2594
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 8 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3386
CseI GACGC 3 cut(s) 71, 524, 2981
Csp6I GTAC 3 cut(s) 156, 2498, 3453
CviAII CATG 9 cut(s) 414, 443, 1784, 1790, 1879, 1957, 2438, 3083, 3266
CviQI GTAC 3 cut(s) 156, 2498, 3453
DinI GGCGCC 1 cut(s) 2084
EaeI YGGCCR 2 cut(s) 3225, 3300
EagI CGGCCG 1 cut(s) 3300
EciI GGCGGA 2 cut(s) 2165, 3325
Ecl136II GAGCTC 1 cut(s) 880
EclXI CGGCCG 1 cut(s) 3300
Eco130I CCWWGG 3 cut(s) 1014, 3228, 3528
Eco147I AGGCCT 1 cut(s) 3527
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3386
Eco52I CGGCCG 1 cut(s) 3300
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 3097
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 4 cut(s) 1255, 1468, 2278, 3386
EcoRI GAATTC 1 cut(s) 3571
EcoRII CCWGG 6 cut(s) 304, 2085, 2557, 2665, 3388, 3456
EcoT14I CCWWGG 3 cut(s) 1014, 3228, 3528
EcoT38I GRGCYC 1 cut(s) 882
EgeI GGCGCC 1 cut(s) 2084
EheI GGCGCC 1 cut(s) 2084
ErhI CCWWGG 3 cut(s) 1014, 3228, 3528
FaeI CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
FalI AAGNNNNNCTT 2 cut(s) 774, 806
FaqI GGGAC 5 cut(s) 1471, 1481, 1701, 2469, 3324
FatI CATG 9 cut(s) 413, 442, 1783, 1789, 1878, 1956, 2437, 3082, 3265
FauI CCCGC 2 cut(s) 2243, 2963
FbaI TGATCA 1 cut(s) 1207
FriOI GRGCYC 1 cut(s) 882
FspBI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
GlaI GCGC 5 cut(s) 195, 240, 1437, 2084, 2593
GsaI CCCAGC 1 cut(s) 2285
GsuI CTGGAG 7 cut(s) 26, 750, 965, 2649, 2674, 3048, 3479
HaeII RGCGCY 2 cut(s) 2086, 2595
HaeIII GGCC 8 cut(s) 714, 1173, 1667, 3102, 3227, 3302, 3527, 3548
HapII CCGG 4 cut(s) 23, 124, 2003, 3042
HgaI GACGC 3 cut(s) 71, 524, 2981
HhaI GCGC 5 cut(s) 196, 241, 1438, 2085, 2594
Hin1I GRCGYC 2 cut(s) 63, 2083
Hin1II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
Hin6I GCGC 5 cut(s) 194, 239, 1436, 2083, 2592
HinP1I GCGC 5 cut(s) 194, 239, 1436, 2083, 2592
HincII GTYRAC 4 cut(s) 61, 367, 1348, 2640
HindII GTYRAC 4 cut(s) 61, 367, 1348, 2640
HindIII AAGCTT 2 cut(s) 543, 1001
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 4 cut(s) 23, 124, 2003, 3042
HphI GGTGA 8 cut(s) 88, 1088, 2153, 2255, 2408, 2598, 3081, 3496
Hpy166II GTNNAC 8 cut(s) 61, 96, 158, 367, 1348, 2540, 2640, 3089
Hpy8I GTNNAC 8 cut(s) 61, 96, 158, 367, 1348, 2540, 2640, 3089
HpyCH4III ACNGT 6 cut(s) 74, 1512, 1522, 3008, 3093, 3280
HpyCH4IV ACGT 3 cut(s) 3163, 3435, 3505
HpySE526I ACGT 3 cut(s) 3163, 3435, 3505
Hsp92I GRCGYC 2 cut(s) 63, 2083
Hsp92II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
HspAI GCGC 5 cut(s) 194, 239, 1436, 2083, 2592
KasI GGCGCC 1 cut(s) 2082
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 3543
LmnI GCTCC 4 cut(s) 628, 799, 2410, 3586
Lsp1109I GCAGC 9 cut(s) 162, 248, 259, 1186, 1258, 1450, 3055, 3091, 3165
MaeI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
MaeII ACGT 3 cut(s) 3163, 3435, 3505
MaeIII GTNAC 9 cut(s) 757, 836, 1234, 1396, 1840, 2257, 2374, 3155, 3428
MbiI CCGCTC 1 cut(s) 3263
MfeI CAATTG 1 cut(s) 3348
MflI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
MhlI GDGCHC 2 cut(s) 882, 1675
MlsI TGGCCA 1 cut(s) 3227
MluNI TGGCCA 1 cut(s) 3227
Mly113I GGCGCC 1 cut(s) 2083
MlyI GAGTC 7 cut(s) 722, 868, 1097, 1288, 1493, 1763, 3155
MmeI TCCRAC 8 cut(s) 633, 1877, 1889, 1987, 2160, 2502, 2964, 3173
Mox20I TGGCCA 1 cut(s) 3227
MroNI GCCGGC 1 cut(s) 22
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 3227
MslI CAYNNNNRTG 2 cut(s) 1788, 1794
Msp20I TGGCCA 1 cut(s) 3227
MspA1I CMGCKG 4 cut(s) 153, 797, 985, 3068
MspCI CTTAAG 2 cut(s) 664, 3340
MspI CCGG 4 cut(s) 23, 124, 2003, 3042
MspR9I CCNGG 7 cut(s) 306, 2003, 2087, 2559, 2667, 3390, 3458
MunI CAATTG 1 cut(s) 3348
Mva1269I GAATGC 2 cut(s) 2329, 3403
MvaI CCWGG 6 cut(s) 306, 2087, 2559, 2667, 3390, 3458
MvnI CGCG 2 cut(s) 241, 2958
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 2083
NciI CCSGG 1 cut(s) 2003
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
NlaIV GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3582
NmeAIII GCCGAG 2 cut(s) 3547, 3557
NmuCI GTSAC 5 cut(s) 757, 836, 1234, 3155, 3428
NspI RCATGY 2 cut(s) 1793, 1960
NspV TTCGAA 1 cut(s) 3541
PaeI GCATGC 1 cut(s) 1793
PagI TCATGA 1 cut(s) 3265
PaqCI CACCTGC 1 cut(s) 2617
PceI AGGCCT 1 cut(s) 3527
PciSI GCTCTTC 1 cut(s) 3543
PctI GAATGC 2 cut(s) 2329, 3403
PdiI GCCGGC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 9 cut(s) 350, 434, 512, 1114, 1301, 1633, 1970, 2711, 3269
PfoI TCCNGGA 2 cut(s) 2665, 3388
PleI GAGTC 7 cut(s) 722, 867, 1096, 1288, 1492, 1763, 3154
PluTI GGCGCC 1 cut(s) 2086
PpsI GAGTC 7 cut(s) 722, 867, 1096, 1288, 1492, 1763, 3154
PpuMI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3386
PshBI ATTAAT 2 cut(s) 1194, 2787
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 4 cut(s) 1255, 1468, 2278, 3386
Psp6I CCWGG 6 cut(s) 304, 2085, 2557, 2665, 3388, 3456
PspFI CCCAGC 1 cut(s) 2281
PspGI CCWGG 6 cut(s) 304, 2085, 2557, 2665, 3388, 3456
PspN4I GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3582
PspPI GGNCC 8 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3386
PspPPI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3386
PsrI GAACNNNNNNTAC 2 cut(s) 2490, 2522
PstNI CAGNNNCTG 2 cut(s) 1937, 2672
PsuI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
PvuII CAGCTG 3 cut(s) 153, 985, 3068
RsaI GTAC 3 cut(s) 157, 2499, 3454
RsaNI GTAC 3 cut(s) 156, 2498, 3453
RseI CAYNNNNRTG 2 cut(s) 1788, 1794
SacI GAGCTC 1 cut(s) 882
SapI GCTCTTC 1 cut(s) 3543
Sau96I GGNCC 8 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3386
SchI GAGTC 7 cut(s) 722, 868, 1097, 1288, 1493, 1763, 3155
ScrFI CCNGG 7 cut(s) 306, 2003, 2087, 2559, 2667, 3390, 3458
SduI GDGCHC 2 cut(s) 882, 1675
SfcI CTRYAG 2 cut(s) 1977, 3279
SfoI GGCGCC 1 cut(s) 2084
SfuI TTCGAA 1 cut(s) 3541
SinI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3386
SmiMI CAYNNNNRTG 2 cut(s) 1788, 1794
SmlI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3381
SmoI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3381
SpeI ACTAGT 3 cut(s) 401, 932, 3023
SphI GCATGC 1 cut(s) 1793
SseBI AGGCCT 1 cut(s) 3527
SspDI GGCGCC 1 cut(s) 2082
SspI AATATT 1 cut(s) 1579
SspMI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
SstI GAGCTC 1 cut(s) 882
StuI AGGCCT 1 cut(s) 3527
StyD4I CCNGG 7 cut(s) 304, 2001, 2085, 2557, 2665, 3388, 3456
StyI CCWWGG 3 cut(s) 1014, 3228, 3528
TaaI ACNGT 6 cut(s) 74, 1512, 1522, 3008, 3093, 3280
TaiI ACGT 3 cut(s) 3166, 3438, 3508
TaqI TCGA 7 cut(s) 246, 427, 1056, 1482, 1531, 3029, 3541
TatI WGTACW 1 cut(s) 155
TauI GCSGC 2 cut(s) 3263, 3302
TfiI GAWTC 9 cut(s) 350, 434, 512, 1114, 1301, 1633, 1970, 2711, 3269
TscAI CASTG 7 cut(s) 77, 456, 774, 1116, 2233, 2301, 3294
TseFI GTSAC 5 cut(s) 757, 836, 1234, 3155, 3428
TseI GCWGC 9 cut(s) 150, 236, 272, 1199, 1271, 1438, 3068, 3079, 3178
Tsp45I GTSAC 5 cut(s) 757, 836, 1234, 3155, 3428
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 7 cut(s) 77, 456, 774, 1116, 2233, 2301, 3294
Vha464I CTTAAG 2 cut(s) 664, 3340
VpaK11BI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3386
VspI ATTAAT 2 cut(s) 1194, 2787
XapI RAATTY 8 cut(s) 289, 315, 570, 1371, 1888, 2034, 2842, 3571
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 2 cut(s) 1793, 1960
XcmI CCANNNNNNNNNTGG 2 cut(s) 1429, 3080
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.