Rh5DG040700

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011014
Physical Location & Seq
Forward (+)
3025081 .. 3029210
4130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG040700.1

Sequence Viewer

Length: 3588 bp
ATGCTGGAGAAGATCGGGTTGCCGGCGAAGCCATCGCTGAGAGGGAATAATTGGGTGGTTGACGCCTCACACTGTCAGGGATGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCATCATTGCCGGAGGTGTGGGGGCATTTTCTGTGGCAGCTGTACACAGCAGAGGATGTTTTTGCGTGGGCAAGGCGATGCGCCTGTGCGGATATGTGAGCCTTGTAAGGAGCTAGAAGAAGCAGCACGGTTCGAGAGATATGGACACAAGAGTAGAGCTGCGAGAGGCGGTTCAAAGTTGACATCAAAGCCTGAGGATGAAATTTTGAACCAGATACTTGGTAATGAAGGGAAGGAATCAGGACAAGAGGTTAACAAGAATGTAGATTCCAGTATGCTGAGGGCAACTAGTAGTGCATCATGTTCAAATAGTCGAGGAGATTCCAGCCATGATGCGGTGGGAGAAATACTTAGAAGTGTTTCTGTTGATAAGTTTAGTCAATTACAGAATGATAGTGAATCTGCTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAAAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCGTTGGAAATATCCTTAAGAAAAAAGCGTAAAAAGGATTTACTCTCTGACAATGTGGCTGACGGCCAGATTAAAGATGACCCTTCCCAATCTGGAAGAAGAAACAGGGTCACTCCTCCAGTGGGTAAAGAAAAAGATGACCTTTCTGCGGAGCTTAAAGAATTAGGGTGGTCCGATATGGATCTCCATGACGAGGATAAGAAAAAAGCGAGTCTGAGTTTGGAGGGTGAGCTCTCTTCTCTTCTGGGAGAAATCTCACAAAAGAGCAATAAAAGTAAGGCTACTAGTGCCATTGACAAAACACAGGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGCAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTTGAAGAACAGGAATTGTTGGGTGAGGCTGAAGAGTCCGATGATGAAATATCTGCACTGATTCGTAGTATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAGGATGGCTTTAATTTTGATAGTCTTATTAATGCTGATGATGATCATATTATAGATAGTAATTTTGAAGTGACAGAAGATGATATGGAGGACCCAGAAATAACTGCTACCTTAAAATCTTTAGGATGGACTCAAGATTCAAATAATCCTCCTGAAACCTCTGCTACCCGGATAGCTTCAGTTGACAAGGAAGCACTATTAAGTGAAATTCAATCCTTAAAAAGAGAGGCTGTAACTCTTAAACGAGCAGGTAATGCCACAGAAGCAATGGCGCAGCTAAAAAAGGCAAAACTGCTTGAAAGGGACCTTGAAAGCATTGAGTCCCAAGAGGGCAATGTCGTGAAACCTTCTGCAACAGTTCATAATCAAACTATTGATAGATCATCAAAGTCATCTATGGTGGATGATGGAAATATTAGTGCAATGGAACATATTGACTTCAAACCTGCGAGGAAGAGTAAGTTAATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAGGGACGATTAGATGAAGCTGAAGAAGAGTTGAAGAGAGGCAGGGTTCTTGAGCATCAGCTTGAAGAGATGGAGAATGGTAAGGACTCAGTTTTGGAACATGAGCATGCCAATGTCTCTGGAAGTCTGTCTTTTGCAGATGAGGAAGGAGAGAATGTAACAGATCAAGATATGTATGATCCAACATATCTTTCCATGCTAAGGAATTTGGGTTGGAATGATGATAATGAAGTTGGAAACTCTTCATCAGCACCTGATAAGAAAATTGATAACATGCAGGCTAGTGAATCTTCTGTAGCACAAGCCCCAAGTGATTTCCCGGTTGGAGGATCAAGGAAAAGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAGGCTCTTGCTCTGAGGCGCCAGGGAGACACCGAGGAAGCAGAAGAAATGCTGAAAAAGGCAAAAGCATTAGAGGGTGAGATGGCGGAGATGGAAGCACCGAAGAAAGTTCAGTCGGACTTTGGTAGGGACAAGGACAATTTCATTGATCCTCTTCCTTTCAGTGTAGAGGAAGAAGGTGATGGCGGGAATGTAACAGAAGATGATATGCAGGACCCAGCACTACTCTCAGTGCTAAAGAGTTTGGGGTGGGAGGATAAAGAGCATTCTGTAGAGGCATCTCCTCTGCAATCTTCTTCCAAACTTGCTGTTGCTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTTTGGATTTGAAAAGAAAGGCTCATGCATTTAGACGAAAGGGACAGACTGAAGAAGCTGAAGAAGTGTTGAAGATGGCAAAGGTACTAGAAGTTCAAATTGAAGAGTTGGAAGCCCCAAAGGGTTTACCAATGGAATTGGGTCCAGGTCGTCCAGACAACCAGAGAATTGCTCAAGGCGCTAGTCAGTCTTCACATGCCCAATCTGGGAACTTTGCAGATTTGTTGACGGGGGATGACTGGAGAGGTTCTCCAGGATCTGCTGAAAAACAATATGATAACCTATCTTATTCTGTTGATTCTGTTAATGCCAGTCCTCCCATTCAGTTGAGATCCTCACAGGAAGATCTGAGAAAAAGAGATGATGCAATAATTAATAAACGGGAAGATACGATTGTAGCCAATGAGAAGCAAGATGCTAAAGAGGCAAATTTGGTTCTGGGATCTTCTTCACAGAAGAATGAAAGTGCCATTCGGCAAGAAATCGTGGCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGAAAACTGACAGAAGCCCGCGAAGAACTTAGGCAGGCAAAAGTGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTCGAGTGATGTGTCCGGTTCTGCTAACAATGTTCCTCCAGCTGCCCAAAAGCAGCATGGTTCACCGTCCTCGGGCCAAAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGATTGAAGAAGCGGAAACAGAGTTTGAATTGGCCAAGGCACTTGAGGCTCAGTTAGAGGAAAGTGCTGCTCATGATCCCACAAGTGTAGCACCAGTGGATGATGCGGCCGTAGAGGGACTTCTTGATCCTGAACTTCTGTCTGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACATCATCTCAAGGTCCCGGAAGACCAGAGCATTCAAAACCCAATGTTGGCAAGAGTGACAACGTGATCCAAGATAGAAGTAACCTGGAGGAACAGATCAAGGCAGAGAAGGTGAAGGCACTAAATCTGAAACGTGCAGGAAAACAAGCCGAGGCCTTAGATGCTCTTCGAAGGGCCAAGATGCTTGAAAAGAAGCTGAATTCCTCGGCTCCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1195

Amino Acids

132.09

Weight (kDa)

5.34

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 1.3e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1403, 1618
AccB1I GGYRCC 1 cut(s) 2082
AccII CGCG 1 cut(s) 2958
AcoI YGGCCR 3 cut(s) 712, 3225, 3300
AcsI RAATTY 7 cut(s) 315, 570, 1371, 1888, 2034, 2842, 3568
AcuI CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
AcyI GRCGYC 2 cut(s) 63, 2083
AfaI GTAC 2 cut(s) 157, 2499
AfiI CCNNNNNNNGG 9 cut(s) 448, 770, 796, 841, 1884, 2009, 2619, 3098, 3416
AflII CTTAAG 2 cut(s) 664, 3340
AhlI ACTAGT 3 cut(s) 401, 932, 3023
AjnI CCWGG 4 cut(s) 2085, 2557, 2665, 3453
AjuI GAANNNNNNNTTGG 2 cut(s) 1858, 1890
Alw21I GWGCWC 2 cut(s) 882, 1675
Alw26I GTCTC 4 cut(s) 1672, 1804, 2085, 3153
AlwNI CAGNNNCTG 2 cut(s) 1937, 2672
Ama87I CYCGRG 1 cut(s) 3097
AoxI GGCC 7 cut(s) 712, 1665, 3100, 3225, 3300, 3522, 3543
ApeKI GCWGC 9 cut(s) 150, 236, 272, 1438, 2378, 3068, 3079, 3178, 3260
ApoI RAATTY 7 cut(s) 315, 570, 1371, 1888, 2034, 2842, 3568
ArsI GACNNNNNNTTYG 2 cut(s) 3175, 3207
AseI ATTAAT 2 cut(s) 1194, 2787
Asp700I GAANNNNTTC 2 cut(s) 472, 1924
AspLEI GCGC 4 cut(s) 196, 1438, 2085, 2594
AspS9I GGNCC 9 cut(s) 819, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
AsuC2I CCSGG 3 cut(s) 1333, 2003, 3387
AsuHPI GGTGA 8 cut(s) 88, 887, 1088, 2153, 2255, 2408, 3081, 3493
AsuII TTCGAA 1 cut(s) 3538
AvaI CYCGRG 1 cut(s) 3097
AvaII GGWCC 6 cut(s) 819, 1255, 1468, 2278, 2555, 3383
AxyI CCTNAGG 2 cut(s) 306, 525
BaeI ACNNNNGTAYC 2 cut(s) 2793, 2826
BalI TGGCCA 1 cut(s) 3227
BanI GGYRCC 1 cut(s) 2082
BanII GRGCYC 1 cut(s) 882
BarI GAAGNNNNNNTAC 2 cut(s) 3297, 3329
BbsI GAAGAC 3 cut(s) 2595, 3009, 3397
Bbv12I GWGCWC 2 cut(s) 882, 1675
BbvCI CCTCAGC 1 cut(s) 392
BbvI GCAGC 9 cut(s) 162, 248, 259, 1450, 2365, 3055, 3091, 3165, 3247
BccI CCATC 9 cut(s) 40, 1163, 1284, 1565, 1747, 2140, 2149, 2240, 2482
BceAI ACGGC 2 cut(s) 727, 3287
BciT130I CCWGG 4 cut(s) 2087, 2559, 2667, 3455
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 3 cut(s) 1333, 2003, 3387
BcoDI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BcuI ACTAGT 3 cut(s) 401, 932, 3023
BfaI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
BfmI CTRYAG 2 cut(s) 1977, 2334
BfoI RGCGCY 2 cut(s) 2086, 2595
BfrI CTTAAG 2 cut(s) 664, 3340
BfuAI ACCTGC 2 cut(s) 1403, 1618
BglII AGATCT 1 cut(s) 2758
Bme1390I CCNGG 7 cut(s) 1333, 2003, 2087, 2559, 2667, 3387, 3455
Bme18I GGWCC 6 cut(s) 819, 1255, 1468, 2278, 2555, 3383
BmeT110I CYCGRG 1 cut(s) 3097
BmgT120I GGNCC 9 cut(s) 819, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
BmiI GGNNCC 7 cut(s) 1257, 1469, 2084, 2280, 2556, 3385, 3579
BmrFI CCNGG 7 cut(s) 1333, 2003, 2087, 2559, 2667, 3387, 3455
BpiI GAAGAC 3 cut(s) 2595, 3009, 3397
BplI GAGNNNNNCTC 4 cut(s) 2569, 2601, 2647, 2679
BpmI CTGGAG 7 cut(s) 26, 750, 965, 2649, 2674, 3048, 3476
Bpu10I CCTNAGC 2 cut(s) 392, 1883
Bpu14I TTCGAA 1 cut(s) 3538
BpuEI CTTGAG 7 cut(s) 653, 1281, 1754, 2571, 3017, 3257, 3363
BpuMI CCSGG 3 cut(s) 1333, 2003, 3387
BsaBI GATNNNNATC 2 cut(s) 828, 1206
BsaHI GRCGYC 2 cut(s) 63, 2083
BsaJI CCNNGG 7 cut(s) 1014, 2086, 2097, 3096, 3228, 3519, 3573
BsaWI WCCGGW 1 cut(s) 3041
BsaXI ACNNNNNCTCC 2 cut(s) 1824, 1854
Bsc4I CCNNNNNNNGG 9 cut(s) 448, 770, 796, 841, 1884, 2009, 2619, 3098, 3416
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
Bse21I CCTNAGG 2 cut(s) 306, 525
Bse3DI GCAATG 4 cut(s) 118, 1437, 1504, 1593
Bse8I GATNNNNATC 2 cut(s) 828, 1206
BseBI CCWGG 4 cut(s) 2087, 2559, 2667, 3455
BseDI CCNNGG 7 cut(s) 1014, 2086, 2097, 3096, 3228, 3519, 3573
BseJI GATNNNNATC 2 cut(s) 828, 1206
BseLI CCNNNNNNNGG 9 cut(s) 448, 770, 796, 841, 1884, 2009, 2619, 3098, 3416
BseMI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BseNI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BseRI GAGGAG 4 cut(s) 444, 753, 2337, 2926
BseX3I CGGCCG 1 cut(s) 3300
BseXI GCAGC 9 cut(s) 162, 248, 259, 1450, 2365, 3055, 3091, 3165, 3247
BseYI CCCAGC 1 cut(s) 2281
BsgI GTGCAG 3 cut(s) 1092, 2364, 3525
Bsh1236I CGCG 1 cut(s) 2958
Bsh1285I CGRYCG 1 cut(s) 3303
BshFI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BshNI GGYRCC 1 cut(s) 2082
BsiEI CGRYCG 1 cut(s) 3303
BsiHKAI GWGCWC 2 cut(s) 882, 1675
BsiHKCI CYCGRG 1 cut(s) 3097
BsiSI CCGG 6 cut(s) 23, 124, 1333, 2003, 3042, 3387
BslFI GGGAC 7 cut(s) 1471, 1481, 1701, 2207, 2469, 3324, 3369
BslI CCNNNNNNNGG 9 cut(s) 448, 770, 796, 841, 1884, 2009, 2619, 3098, 3416
BsmAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BsmFI GGGAC 7 cut(s) 1471, 1481, 1701, 2207, 2469, 3324, 3369
BsmI GAATGC 2 cut(s) 2329, 3400
BsnI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BsoBI CYCGRG 1 cut(s) 3097
Bsp119I TTCGAA 1 cut(s) 3538
Bsp1286I GDGCHC 2 cut(s) 882, 1675
Bsp1407I TGTACA 1 cut(s) 155
BspANI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BspFNI CGCG 1 cut(s) 2958
BspHI TCATGA 1 cut(s) 3265
BspLI GGNNCC 7 cut(s) 1257, 1469, 2084, 2280, 2556, 3385, 3579
BspMI ACCTGC 2 cut(s) 1403, 1618
BspQI GCTCTTC 1 cut(s) 3540
BspT104I TTCGAA 1 cut(s) 3538
BspT107I GGYRCC 1 cut(s) 2082
BspTI CTTAAG 2 cut(s) 664, 3340
BsrDI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BsrFI RCCGGY 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 155
BsrI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 7 cut(s) 1014, 2086, 2097, 3096, 3228, 3519, 3573
BssNI GRCGYC 2 cut(s) 63, 2083
BssT1I CCWWGG 2 cut(s) 1014, 3228
Bst2UI CCWGG 4 cut(s) 2087, 2559, 2667, 3455
Bst4CI ACNGT 5 cut(s) 74, 243, 1522, 3008, 3093
BstACI GRCGYC 2 cut(s) 63, 2083
BstAFI CTTAAG 2 cut(s) 664, 3340
BstAPI GCANNNNNTGC 1 cut(s) 1418
BstAUI TGTACA 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 3538
BstFNI CGCG 1 cut(s) 2958
BstH2I RGCGCY 2 cut(s) 2086, 2595
BstHHI GCGC 4 cut(s) 196, 1438, 2085, 2594
BstMAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BstMCI CGRYCG 1 cut(s) 3303
BstNI CCWGG 4 cut(s) 2087, 2559, 2667, 3455
BstNSI RCATGY 3 cut(s) 1793, 1960, 2612
BstSCI CCNGG 7 cut(s) 1331, 2001, 2085, 2557, 2665, 3385, 3453
BstSFI CTRYAG 2 cut(s) 1977, 2334
BstUI CGCG 1 cut(s) 2958
BstV1I GCAGC 9 cut(s) 162, 248, 259, 1450, 2365, 3055, 3091, 3165, 3247
BstV2I GAAGAC 3 cut(s) 2595, 3009, 3397
BstX2I RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
BstZI CGGCCG 1 cut(s) 3300
Bsu36I CCTNAGG 2 cut(s) 306, 525
BsuRI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BtgZI GCGATG 2 cut(s) 18, 204
BtsIMutI CAGTG 6 cut(s) 70, 774, 1109, 2233, 2301, 3294
BveI ACCTGC 2 cut(s) 1403, 1618
CaiI CAGNNNCTG 2 cut(s) 1937, 2672
CciI TCATGA 1 cut(s) 3265
CfoI GCGC 4 cut(s) 196, 1438, 2085, 2594
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 9 cut(s) 819, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
CseI GACGC 3 cut(s) 71, 524, 2981
Csp6I GTAC 2 cut(s) 156, 2498
CviQI GTAC 2 cut(s) 156, 2498
DinI GGCGCC 1 cut(s) 2084
EaeI YGGCCR 3 cut(s) 712, 3225, 3300
EagI CGGCCG 1 cut(s) 3300
EciI GGCGGA 1 cut(s) 2165
Ecl136II GAGCTC 1 cut(s) 880
EclXI CGGCCG 1 cut(s) 3300
Eco130I CCWWGG 2 cut(s) 1014, 3228
Eco147I AGGCCT 1 cut(s) 3524
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 6 cut(s) 819, 1255, 1468, 2278, 2555, 3383
Eco52I CGGCCG 1 cut(s) 3300
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
Eco81I CCTNAGG 2 cut(s) 306, 525
Eco88I CYCGRG 1 cut(s) 3097
EcoICRI GAGCTC 1 cut(s) 880
EcoO109I RGGNCCY 4 cut(s) 1255, 1468, 2278, 3383
EcoRI GAATTC 1 cut(s) 3568
EcoRII CCWGG 4 cut(s) 2085, 2557, 2665, 3453
EcoT14I CCWWGG 2 cut(s) 1014, 3228
EcoT22I ATGCAT 1 cut(s) 2443
EcoT38I GRGCYC 1 cut(s) 882
EgeI GGCGCC 1 cut(s) 2084
EheI GGCGCC 1 cut(s) 2084
ErhI CCWWGG 2 cut(s) 1014, 3228
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1798, 1830
FaqI GGGAC 7 cut(s) 1471, 1481, 1701, 2207, 2469, 3324, 3369
FauI CCCGC 2 cut(s) 2243, 2963
FbaI TGATCA 1 cut(s) 1207
FriOI GRGCYC 1 cut(s) 882
FspBI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
GlaI GCGC 4 cut(s) 195, 1437, 2084, 2593
GsaI CCCAGC 1 cut(s) 2285
GsuI CTGGAG 7 cut(s) 26, 750, 965, 2649, 2674, 3048, 3476
HaeII RGCGCY 2 cut(s) 2086, 2595
HaeIII GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
HapII CCGG 6 cut(s) 23, 124, 1333, 2003, 3042, 3387
HgaI GACGC 3 cut(s) 71, 524, 2981
HhaI GCGC 4 cut(s) 196, 1438, 2085, 2594
Hin1I GRCGYC 2 cut(s) 63, 2083
Hin6I GCGC 4 cut(s) 194, 1436, 2083, 2592
HinP1I GCGC 4 cut(s) 194, 1436, 2083, 2592
HincII GTYRAC 5 cut(s) 61, 294, 367, 1348, 2640
HindII GTYRAC 5 cut(s) 61, 294, 367, 1348, 2640
HindIII AAGCTT 2 cut(s) 543, 1001
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 6 cut(s) 23, 124, 1333, 2003, 3042, 3387
HphI GGTGA 8 cut(s) 88, 887, 1088, 2153, 2255, 2408, 3081, 3493
Hpy166II GTNNAC 9 cut(s) 61, 96, 158, 294, 367, 1348, 2540, 2640, 3089
Hpy8I GTNNAC 9 cut(s) 61, 96, 158, 294, 367, 1348, 2540, 2640, 3089
HpyCH4III ACNGT 5 cut(s) 74, 243, 1522, 3008, 3093
HpyCH4IV ACGT 3 cut(s) 3163, 3432, 3502
HpySE526I ACGT 3 cut(s) 3163, 3432, 3502
Hsp92I GRCGYC 2 cut(s) 63, 2083
HspAI GCGC 4 cut(s) 194, 1436, 2083, 2592
KasI GGCGCC 1 cut(s) 2082
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 3540
LmnI GCTCC 5 cut(s) 223, 628, 799, 2410, 3583
Lsp1109I GCAGC 9 cut(s) 162, 248, 259, 1450, 2365, 3055, 3091, 3165, 3247
MaeI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
MaeII ACGT 3 cut(s) 3163, 3432, 3502
MaeIII GTNAC 8 cut(s) 757, 1234, 1396, 1840, 2257, 3155, 3425, 3449
MfeI CAATTG 1 cut(s) 3348
MflI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
MhlI GDGCHC 2 cut(s) 882, 1675
MlsI TGGCCA 1 cut(s) 3227
MluNI TGGCCA 1 cut(s) 3227
Mly113I GGCGCC 1 cut(s) 2083
MlyI GAGTC 6 cut(s) 868, 1097, 1288, 1493, 1763, 3155
MmeI TCCRAC 9 cut(s) 633, 1877, 1889, 1897, 1987, 2160, 2502, 2964, 3173
Mox20I TGGCCA 1 cut(s) 3227
Mph1103I ATGCAT 1 cut(s) 2443
MroNI GCCGGC 1 cut(s) 22
MroXI GAANNNNTTC 2 cut(s) 472, 1924
MscI TGGCCA 1 cut(s) 3227
MslI CAYNNNNRTG 2 cut(s) 1788, 1794
Msp20I TGGCCA 1 cut(s) 3227
MspA1I CMGCKG 3 cut(s) 153, 985, 3068
MspCI CTTAAG 2 cut(s) 664, 3340
MspI CCGG 6 cut(s) 23, 124, 1333, 2003, 3042, 3387
MspR9I CCNGG 7 cut(s) 1333, 2003, 2087, 2559, 2667, 3387, 3455
MunI CAATTG 1 cut(s) 3348
Mva1269I GAATGC 2 cut(s) 2329, 3400
MvaI CCWGG 4 cut(s) 2087, 2559, 2667, 3455
MvnI CGCG 1 cut(s) 2958
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 2083
NciI CCSGG 3 cut(s) 1333, 2003, 3387
NgoMIV GCCGGC 1 cut(s) 22
NlaIV GGNNCC 7 cut(s) 1257, 1469, 2084, 2280, 2556, 3385, 3579
NmeAIII GCCGAG 2 cut(s) 3544, 3554
NmuCI GTSAC 4 cut(s) 757, 1234, 3155, 3425
NsiI ATGCAT 1 cut(s) 2443
NspI RCATGY 3 cut(s) 1793, 1960, 2612
NspV TTCGAA 1 cut(s) 3538
PaeI GCATGC 1 cut(s) 1793
PagI TCATGA 1 cut(s) 3265
PceI AGGCCT 1 cut(s) 3524
PciSI GCTCTTC 1 cut(s) 3540
PctI GAATGC 2 cut(s) 2329, 3400
PdiI GCCGGC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 472, 1924
PfeI GAWTC 9 cut(s) 350, 380, 434, 512, 1114, 1301, 1633, 1970, 2711
PfoI TCCNGGA 2 cut(s) 2665, 3385
PleI GAGTC 6 cut(s) 867, 1096, 1288, 1492, 1763, 3154
PluTI GGCGCC 1 cut(s) 2086
PpsI GAGTC 6 cut(s) 867, 1096, 1288, 1492, 1763, 3154
PpuMI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
PshBI ATTAAT 2 cut(s) 1194, 2787
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
Psp6I CCWGG 4 cut(s) 2085, 2557, 2665, 3453
PspFI CCCAGC 1 cut(s) 2281
PspGI CCWGG 4 cut(s) 2085, 2557, 2665, 3453
PspN4I GGNNCC 7 cut(s) 1257, 1469, 2084, 2280, 2556, 3385, 3579
PspPI GGNCC 9 cut(s) 819, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
PspPPI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
PsrI GAACNNNNNNTAC 2 cut(s) 2490, 2522
PstNI CAGNNNCTG 2 cut(s) 1937, 2672
PsuI RGATCY 5 cut(s) 829, 2669, 2744, 2758, 2855
PvuII CAGCTG 3 cut(s) 153, 985, 3068
RsaI GTAC 2 cut(s) 157, 2499
RsaNI GTAC 2 cut(s) 156, 2498
RseI CAYNNNNRTG 2 cut(s) 1788, 1794
SacI GAGCTC 1 cut(s) 882
SapI GCTCTTC 1 cut(s) 3540
Sau96I GGNCC 9 cut(s) 819, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
SchI GAGTC 6 cut(s) 868, 1097, 1288, 1493, 1763, 3155
ScrFI CCNGG 7 cut(s) 1333, 2003, 2087, 2559, 2667, 3387, 3455
SduI GDGCHC 2 cut(s) 882, 1675
SfcI CTRYAG 2 cut(s) 1977, 2334
SfoI GGCGCC 1 cut(s) 2084
SfuI TTCGAA 1 cut(s) 3538
SinI GGWCC 6 cut(s) 819, 1255, 1468, 2278, 2555, 3383
SmiMI CAYNNNNRTG 2 cut(s) 1788, 1794
SmlI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3378
SmoI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3378
SpeI ACTAGT 3 cut(s) 401, 932, 3023
SphI GCATGC 1 cut(s) 1793
SseBI AGGCCT 1 cut(s) 3524
SspDI GGCGCC 1 cut(s) 2082
SspI AATATT 1 cut(s) 1579
SspMI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
SstI GAGCTC 1 cut(s) 882
StuI AGGCCT 1 cut(s) 3524
StyD4I CCNGG 7 cut(s) 1331, 2001, 2085, 2557, 2665, 3385, 3453
StyI CCWWGG 2 cut(s) 1014, 3228
TaaI ACNGT 5 cut(s) 74, 243, 1522, 3008, 3093
TaiI ACGT 3 cut(s) 3166, 3435, 3505
TaqI TCGA 4 cut(s) 246, 427, 3029, 3538
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 3302
TfiI GAWTC 9 cut(s) 350, 380, 434, 512, 1114, 1301, 1633, 1970, 2711
TscAI CASTG 6 cut(s) 77, 774, 1116, 2233, 2301, 3294
TseFI GTSAC 4 cut(s) 757, 1234, 3155, 3425
TseI GCWGC 9 cut(s) 150, 236, 272, 1438, 2378, 3068, 3079, 3178, 3260
Tsp45I GTSAC 4 cut(s) 757, 1234, 3155, 3425
TspRI CASTG 6 cut(s) 77, 774, 1116, 2233, 2301, 3294
Vha464I CTTAAG 2 cut(s) 664, 3340
VpaK11BI GGWCC 6 cut(s) 819, 1255, 1468, 2278, 2555, 3383
VspI ATTAAT 2 cut(s) 1194, 2787
XapI RAATTY 7 cut(s) 315, 570, 1371, 1888, 2034, 2842, 3568
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 3 cut(s) 1793, 1960, 2612
XcmI CCANNNNNNNNNTGG 2 cut(s) 1429, 3080
XmnI GAANNNNTTC 2 cut(s) 472, 1924
XspI CTAG 9 cut(s) 227, 402, 933, 1043, 1965, 2501, 2595, 3024, 3152
Zsp2I ATGCAT 1 cut(s) 2443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.