Rh5AG041000

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011014
Physical Location & Seq
Forward (+)
3069123 .. 3073885
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG041000.1

Sequence Viewer

Length: 3564 bp
ATGTACATTGTGGAATTTCAGCATCATTGCCGGAGGTGTGGGGGCATTTTCTGTGGCAGCTGTACACAGCAGAGGATGTTTTTGCGTGGGCAAGGCGATGCGCCTGTGCGGATATGTGAGCCTTGTAAGAAGCTAGAAGAAGCAGGGCGGTTCGAGAGATATGGACACAAGAGTAGAGCTGCGAGAGGCAGTTCAAATTTGACATCAAAGCCTGAGGATGAGATTTTGAACCAGATACTTGGTAATGAAGGGAAGGAATCAGGACAAGAGGTTAACAAGAATGTAGTTTCCAGTATGCTGAGGGCAACTAGCAGTGCATCATGTTCAAATAGTCGAGGAGATTCCAGCCATGATGCGGTGGGAGAAATACTTAGAAGTGTTTCTGTTGATAAGTTTAGTCAATTACAGAATGATAGTGAATCTGTTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAAAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTTCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCGTTGGAAATATCCTTAAGAAAAAAGCGTAAAAAGGATTTACTCTCTGACAATGTGGCTGAGGGCCAGATTAAAGATGACTCTTCCCAATCTGGAAGAAGAAACAGGGTCACTCCTCCAGTGGGTAAAGAAAAAGATGACCTTTCAGCGGAGCTTAAAGAATTAGGGTGGTCTGATATGGATCTCCGTGACGAGAATAAAAAACAAGCGAGTCTGACTGTGGAGGGCGAGCTGTCTTCTCTTCTGGGAGAAATCTCACAAAAGAGCAATAAAAGTAAGGCTACTAGTGCCATTGACAAAACACAGGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGCAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTTGAAGAACAGGAATTGTTGGGTGAGGCTGAAGAGTCCGATGATGAAATATCTGCACTGATTCGTAGTATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAGGATGGCTTTAATTTTGATAGTCTTATTAATGCTGCTGATGACCATATTATAGATAGTAATTTTGAAGTGACAGAAGAGGATATGGAGGACCCAGAAATAACTGCTGCCTTAAAATCTTTAGGATGGACTCAAGATTCAAATAATCCTCCTGAAACCTCTGCTACCCAGATAGCTTCAGTTGACAAGGAAGCACTATTAAGTGAAATTCAATCCTTAAAAAGAGAGGCTGTAACTCTTAAACGAGCAGGTAATGCCACAGAAGCAATGGTGCAGCTAAAAAAGGCAAAACTGCTTGAAAGGGACCTTGAAAGCATTGAGTCCCAAGAGGGCAATGTCGTGAAACCTTCTGCAACAGTTCATAATCAAACTATTGATAGATCATCAAAGTCATCTATGGTGGATGATGGAAATATTAGTGCAATGGAACATATTGACTTCAAACCTGCGAGGAAGAGTAAGTTAATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAGGGACGATTAGATGAAGCTGAAGAAGAGTTGAAGAGAGGCAGGATTCTTGAGCATCAGCTTGAAGAGATGGAGAATGGTAAGGACTCAGTTTTGGAACATGAGCATGCCAATGTCTCTGGAAGTCTGTCTTTTGCAGATGAGGAAGGAGAGAATGTAACAGATCAAGATATGTATGATCCAACATATCTTTCCATGCTAAGGAATTTGGGTTGGAATGATGATAATGAAGTTGCAAACTCTTCATCAGCACCTGATAAGAAAATTTATGACATGCAGGCTAGTGAATCTTCTGGAGCACAAGCCCGAAGTGATTTCCCGGTTGGAGGATCAAGGAAAAGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAGGCTCTTGCTCTGAGGCGCCAGGGAGACACTGAGGAAGCAGAAGAAATGCTGAAAAAGGCAAAAGCATTAGAGGGGGAGATGGCGGAGATGGAAGCACCGAAGAAAGTTCAGTCGGACTTTGGTAGGGACAAGGACAATTTCATTGATCCTATTCGTTTCAGTGTAGAGGAAGAAGGTGATGGCGGGGATGTAACAGAAAATGATATGCAGGACCCAGCACTACTCTCAGTGCTAAAGAGTTTGGGGTGGGAGGATAAAGAGCATTCTGTAGAGGCATCTCCTCTGCAATCTTCTTCCAAACTTGCTGTTGCTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTTTGGATTTGAAAAGAAAGGCTCATGCATTTAGACGAAAGGGGCAGACTGAAGAAGCTGAAGAAGTGTTGAAGATGGCAAAGGTACTAGAAGTTCAAATTGAAGAGTTGGAAGCCCCAAAAGGTTTACCAATGGAATTGGGTCCAGGTCGTCCAGACAACCAGAGAATTGCTCAAGGCGCTAGTCAGTCTTCACCTGCCCAATCTGGGAACTTCGCAGATTTGTTGACGGGGGATGACTGGAGAGGTTCTCCAGGATCTGCTGAAAGACAATATGATAACCTATCTTATTCTGTTGATTCTGTTAATGCCAGTCCTCCCATTCAGTTGAGATCCTCACAGGAAGATCTGAGAAAACGAGATGATGCAATAATTAATAAACGGGAAGATACGATTGTAGCCAATGAGAAGCAAGATGCTAAAGAGGTCAATTTGGTTCTGGGATCTTCTTCACAGAAGAATGAAAGTGCCAATGAGAAGCAAGATGCTACAGAGGTCAATTTGGTTCTGGGATCATCTTCACAGAAGAATGAAAGTGCCATTCGGCAAGAAATCGTGGCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGAAAACTGACAGAAGCCCGCGAAGAACTTAGGCAGGCTAAAGTGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTCGAGTGATGTGTCCGGTTCTGCTAACAATGTTCCTCCAGCTGCCCAAAAGCAGCATGGATCACCGTCCTCGGGCCAAAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGATTGAAGAAGCGGAAGCAGAGTTTGAATTGGCCAAGGTACTTGAGGCTCAGTTAGAGGAAAGTGCTACTCATGATTCCACTAGTGTAGCACCAGTGGATGATGCGGCCGTAGAGGGACTTCTTGATCCTGAACTTCTGTCTGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACATCATCTCAAGGTCCTGGAAGACCAGAGCATTCAAAACCCAGTGTTGGCAAGAGTGACGATGTAATCCAAGATAGAAGTAATCTGGAGGAACAGATCAAGGCAGAGAAGGTGAAGGCACTAAATCTGAAACGTGCAGGAAAACAAGCCGAGGCCTTGGATGCTCTTCGAAGGGCCAAGATGCTTGAAAAGAAGCTGAATTCCTCGGCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1187

Amino Acids

131.36

Weight (kDa)

5.26

Isoelectric Point (pI)

57.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 6 - 44 2.8e-09 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2524
Acc36I ACCTGC 3 cut(s) 1310, 1525, 2524
AccB1I GGYRCC 1 cut(s) 1989
AccII CGCG 1 cut(s) 2934
AciI CCGC 9 cut(s) 109, 148, 356, 704, 2057, 2157, 2932, 3182, 3275
AcoI YGGCCR 2 cut(s) 3201, 3276
AcsI RAATTY 8 cut(s) 14, 196, 477, 1278, 1795, 1854, 1941, 3544
AcuI CTGAAG 7 cut(s) 914, 1011, 1233, 1632, 2391, 2400, 3170
AcyI GRCGYC 1 cut(s) 1990
AfaI GTAC 4 cut(s) 5, 64, 2406, 3210
AfiI CCNNNNNNNGG 8 cut(s) 355, 677, 703, 1791, 1916, 2526, 3074, 3392
AflII CTTAAG 2 cut(s) 571, 3316
AhlI ACTAGT 3 cut(s) 839, 2999, 3251
AjnI CCWGG 4 cut(s) 1992, 2464, 2572, 3361
AjuI GAANNNNNNNTTGG 2 cut(s) 1765, 1797
Alw21I GWGCWC 2 cut(s) 1582, 1891
Alw26I GTCTC 4 cut(s) 1579, 1711, 1992, 3129
AlwNI CAGNNNCTG 2 cut(s) 1844, 2579
Ama87I CYCGRG 1 cut(s) 3073
AoxI GGCC 7 cut(s) 619, 1572, 3076, 3201, 3276, 3498, 3519
ApeKI GCWGC 9 cut(s) 57, 179, 1106, 1178, 1345, 2285, 3044, 3055, 3154
ApoI RAATTY 8 cut(s) 14, 196, 477, 1278, 1795, 1854, 1941, 3544
ArsI GACNNNNNNTTYG 2 cut(s) 3151, 3183
AseI ATTAAT 2 cut(s) 1101, 2694
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 3 cut(s) 103, 1992, 2501
AspS9I GGNCC 9 cut(s) 619, 1162, 1375, 1573, 2185, 2462, 3076, 3359, 3519
AsuC2I CCSGG 1 cut(s) 1910
AsuHPI GGTGA 6 cut(s) 995, 2162, 2315, 2505, 3057, 3469
AsuII TTCGAA 1 cut(s) 3514
AvaI CYCGRG 1 cut(s) 3073
AvaII GGWCC 5 cut(s) 1162, 1375, 2185, 2462, 3359
AxyI CCTNAGG 2 cut(s) 213, 432
BaeI ACNNNNGTAYC 2 cut(s) 2700, 2733
BalI TGGCCA 1 cut(s) 3203
BanI GGYRCC 1 cut(s) 1989
BarI GAAGNNNNNNTAC 2 cut(s) 3273, 3305
BbsI GAAGAC 5 cut(s) 492, 783, 2502, 2985, 3373
Bbv12I GWGCWC 2 cut(s) 1582, 1891
BbvCI CCTCAGC 2 cut(s) 299, 615
BbvI GCAGC 9 cut(s) 69, 166, 1093, 1165, 1357, 2272, 3031, 3067, 3141
BccI CCATC 8 cut(s) 1070, 1191, 1472, 1654, 2047, 2056, 2147, 2389
BceAI ACGGC 1 cut(s) 3263
BciT130I CCWGG 4 cut(s) 1994, 2466, 2574, 3363
BcnI CCSGG 1 cut(s) 1910
BcoDI GTCTC 4 cut(s) 1579, 1711, 1992, 3129
BcuI ACTAGT 3 cut(s) 839, 2999, 3251
BfmI CTRYAG 2 cut(s) 2241, 2808
BfoI RGCGCY 2 cut(s) 1993, 2502
BfrI CTTAAG 2 cut(s) 571, 3316
BfuAI ACCTGC 3 cut(s) 1310, 1525, 2524
BglII AGATCT 1 cut(s) 2665
Bme1390I CCNGG 5 cut(s) 1910, 1994, 2466, 2574, 3363
Bme18I GGWCC 5 cut(s) 1162, 1375, 2185, 2462, 3359
BmeT110I CYCGRG 1 cut(s) 3073
BmgT120I GGNCC 9 cut(s) 619, 1162, 1375, 1573, 2185, 2462, 3076, 3359, 3519
BmiI GGNNCC 6 cut(s) 1164, 1376, 1991, 2187, 2463, 3555
BmrFI CCNGG 5 cut(s) 1910, 1994, 2466, 2574, 3363
BmrI ACTGGG 1 cut(s) 3381
BmuI ACTGGG 1 cut(s) 3381
BpiI GAAGAC 5 cut(s) 492, 783, 2502, 2985, 3373
BplI GAGNNNNNCTC 4 cut(s) 2476, 2508, 2554, 2586
BpmI CTGGAG 7 cut(s) 657, 872, 1905, 2556, 2581, 3024, 3452
Bpu10I CCTNAGC 3 cut(s) 299, 615, 1790
Bpu14I TTCGAA 1 cut(s) 3514
BpuEI CTTGAG 7 cut(s) 560, 1188, 1661, 2478, 2993, 3233, 3339
BpuMI CCSGG 1 cut(s) 1910
BsaBI GATNNNNATC 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 1990
BsaJI CCNNGG 7 cut(s) 921, 1993, 3072, 3204, 3495, 3501, 3549
BsaWI WCCGGW 1 cut(s) 3017
BsaXI ACNNNNNCTCC 2 cut(s) 1731, 1761
Bsc4I CCNNNNNNNGG 8 cut(s) 355, 677, 703, 1791, 1916, 2526, 3074, 3392
Bse1I ACTGG 7 cut(s) 291, 674, 2564, 2631, 3093, 3263, 3387
Bse21I CCTNAGG 2 cut(s) 213, 432
Bse3DI GCAATG 4 cut(s) 25, 1344, 1411, 1500
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 4 cut(s) 1994, 2466, 2574, 3363
BseDI CCNNGG 7 cut(s) 921, 1993, 3072, 3204, 3495, 3501, 3549
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 8 cut(s) 355, 677, 703, 1791, 1916, 2526, 3074, 3392
BseMI GCAATG 4 cut(s) 25, 1344, 1411, 1500
BseNI ACTGG 7 cut(s) 291, 674, 2564, 2631, 3093, 3263, 3387
BseRI GAGGAG 4 cut(s) 351, 660, 2244, 2902
BseX3I CGGCCG 1 cut(s) 3276
BseXI GCAGC 9 cut(s) 69, 166, 1093, 1165, 1357, 2272, 3031, 3067, 3141
BseYI CCCAGC 1 cut(s) 2188
BsgI GTGCAG 4 cut(s) 999, 1364, 2271, 3501
Bsh1236I CGCG 1 cut(s) 2934
Bsh1285I CGRYCG 1 cut(s) 3279
BshFI GGCC 7 cut(s) 621, 1574, 3078, 3203, 3278, 3500, 3521
BshNI GGYRCC 1 cut(s) 1989
BsiEI CGRYCG 1 cut(s) 3279
BsiHKAI GWGCWC 2 cut(s) 1582, 1891
BsiHKCI CYCGRG 1 cut(s) 3073
BsiSI CCGG 3 cut(s) 31, 1910, 3018
BslFI GGGAC 5 cut(s) 1378, 1388, 1608, 2114, 3300
BslI CCNNNNNNNGG 8 cut(s) 355, 677, 703, 1791, 1916, 2526, 3074, 3392
BsmAI GTCTC 4 cut(s) 1579, 1711, 1992, 3129
BsmFI GGGAC 5 cut(s) 1378, 1388, 1608, 2114, 3300
BsmI GAATGC 2 cut(s) 2236, 3376
BsnI GGCC 7 cut(s) 621, 1574, 3078, 3203, 3278, 3500, 3521
BsoBI CYCGRG 1 cut(s) 3073
Bsp119I TTCGAA 1 cut(s) 3514
Bsp1286I GDGCHC 2 cut(s) 1582, 1891
Bsp1407I TGTACA 2 cut(s) 3, 62
BspACI CCGC 9 cut(s) 109, 148, 356, 704, 2057, 2157, 2932, 3182, 3275
BspANI GGCC 7 cut(s) 621, 1574, 3078, 3203, 3278, 3500, 3521
BspFNI CGCG 1 cut(s) 2934
BspHI TCATGA 1 cut(s) 3241
BspLI GGNNCC 6 cut(s) 1164, 1376, 1991, 2187, 2463, 3555
BspMI ACCTGC 3 cut(s) 1310, 1525, 2524
BspQI GCTCTTC 1 cut(s) 3516
BspT104I TTCGAA 1 cut(s) 3514
BspT107I GGYRCC 1 cut(s) 1989
BspTI CTTAAG 2 cut(s) 571, 3316
BsrDI GCAATG 4 cut(s) 25, 1344, 1411, 1500
BsrGI TGTACA 2 cut(s) 3, 62
BsrI ACTGG 7 cut(s) 291, 674, 2564, 2631, 3093, 3263, 3387
BssECI CCNNGG 7 cut(s) 921, 1993, 3072, 3204, 3495, 3501, 3549
BssNI GRCGYC 1 cut(s) 1990
BssT1I CCWWGG 3 cut(s) 921, 3204, 3501
Bst2UI CCWGG 4 cut(s) 1994, 2466, 2574, 3363
Bst4CI ACNGT 4 cut(s) 775, 1429, 2984, 3069
BstACI GRCGYC 1 cut(s) 1990
BstAFI CTTAAG 2 cut(s) 571, 3316
BstAPI GCANNNNNTGC 1 cut(s) 1325
BstAUI TGTACA 2 cut(s) 3, 62
BstBI TTCGAA 1 cut(s) 3514
BstC8I GCNNGC 9 cut(s) 450, 549, 785, 908, 912, 1698, 1869, 2932, 2949
BstFNI CGCG 1 cut(s) 2934
BstH2I RGCGCY 2 cut(s) 1993, 2502
BstHHI GCGC 3 cut(s) 103, 1992, 2501
BstMAI GTCTC 4 cut(s) 1579, 1711, 1992, 3129
BstMCI CGRYCG 1 cut(s) 3279
BstMWI GCNNNNNNNGC 9 cut(s) 515, 544, 842, 1325, 1334, 2285, 2498, 3320, 3506
BstNI CCWGG 4 cut(s) 1994, 2466, 2574, 3363
BstNSI RCATGY 2 cut(s) 1700, 1867
BstSCI CCNGG 5 cut(s) 1908, 1992, 2464, 2572, 3361
BstSFI CTRYAG 2 cut(s) 2241, 2808
BstUI CGCG 1 cut(s) 2934
BstV1I GCAGC 9 cut(s) 69, 166, 1093, 1165, 1357, 2272, 3031, 3067, 3141
BstV2I GAAGAC 5 cut(s) 492, 783, 2502, 2985, 3373
BstX2I RGATCY 5 cut(s) 736, 2576, 2651, 2665, 2762
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 5 cut(s) 736, 2576, 2651, 2665, 2762
BstZI CGGCCG 1 cut(s) 3276
Bsu36I CCTNAGG 2 cut(s) 213, 432
BsuRI GGCC 7 cut(s) 621, 1574, 3078, 3203, 3278, 3500, 3521
BtgZI GCGATG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 8 cut(s) 319, 681, 1016, 2001, 2140, 2208, 3270, 3394
BveI ACCTGC 3 cut(s) 1310, 1525, 2524
Cac8I GCNNGC 9 cut(s) 450, 549, 785, 908, 912, 1698, 1869, 2932, 2949
CaiI CAGNNNCTG 2 cut(s) 1844, 2579
CciI TCATGA 1 cut(s) 3241
CfoI GCGC 3 cut(s) 103, 1992, 2501
Cfr13I GGNCC 9 cut(s) 619, 1162, 1375, 1573, 2185, 2462, 3076, 3359, 3519
CseI GACGC 2 cut(s) 431, 2957
Csp6I GTAC 4 cut(s) 4, 63, 2405, 3209
CviAII CATG 9 cut(s) 321, 350, 1691, 1697, 1786, 1864, 2345, 3059, 3242
CviQI GTAC 4 cut(s) 4, 63, 2405, 3209
DinI GGCGCC 1 cut(s) 1991
EaeI YGGCCR 2 cut(s) 3201, 3276
EagI CGGCCG 1 cut(s) 3276
EciI GGCGGA 1 cut(s) 2072
EclXI CGGCCG 1 cut(s) 3276
Eco130I CCWWGG 3 cut(s) 921, 3204, 3501
Eco147I AGGCCT 1 cut(s) 3500
Eco47I GGWCC 5 cut(s) 1162, 1375, 2185, 2462, 3359
Eco52I CGGCCG 1 cut(s) 3276
Eco57I CTGAAG 7 cut(s) 914, 1011, 1233, 1632, 2391, 2400, 3170
Eco81I CCTNAGG 2 cut(s) 213, 432
Eco88I CYCGRG 1 cut(s) 3073
EcoO109I RGGNCCY 4 cut(s) 1162, 1375, 2185, 3359
EcoRI GAATTC 1 cut(s) 3544
EcoRII CCWGG 4 cut(s) 1992, 2464, 2572, 3361
EcoT14I CCWWGG 3 cut(s) 921, 3204, 3501
EcoT22I ATGCAT 1 cut(s) 2350
EgeI GGCGCC 1 cut(s) 1991
EheI GGCGCC 1 cut(s) 1991
ErhI CCWWGG 3 cut(s) 921, 3204, 3501
FaeI CATG 9 cut(s) 324, 353, 1694, 1700, 1789, 1867, 2348, 3062, 3245
FalI AAGNNNNNCTT 4 cut(s) 681, 713, 1705, 1737
FaqI GGGAC 5 cut(s) 1378, 1388, 1608, 2114, 3300
FatI CATG 9 cut(s) 320, 349, 1690, 1696, 1785, 1863, 2344, 3058, 3241
FauI CCCGC 2 cut(s) 2150, 2939
GlaI GCGC 3 cut(s) 102, 1991, 2500
GsaI CCCAGC 1 cut(s) 2192
GsuI CTGGAG 7 cut(s) 657, 872, 1905, 2556, 2581, 3024, 3452
HaeII RGCGCY 2 cut(s) 1993, 2502
HaeIII GGCC 7 cut(s) 621, 1574, 3078, 3203, 3278, 3500, 3521
HapII CCGG 3 cut(s) 31, 1910, 3018
HgaI GACGC 2 cut(s) 431, 2957
HhaI GCGC 3 cut(s) 103, 1992, 2501
Hin1I GRCGYC 1 cut(s) 1990
Hin1II CATG 9 cut(s) 324, 353, 1694, 1700, 1789, 1867, 2348, 3062, 3245
Hin6I GCGC 3 cut(s) 101, 1990, 2499
HinP1I GCGC 3 cut(s) 101, 1990, 2499
HincII GTYRAC 3 cut(s) 274, 1255, 2547
HindII GTYRAC 3 cut(s) 274, 1255, 2547
HindIII AAGCTT 2 cut(s) 450, 908
HpaI GTTAAC 1 cut(s) 274
HpaII CCGG 3 cut(s) 31, 1910, 3018
HphI GGTGA 6 cut(s) 995, 2162, 2315, 2505, 3057, 3469
Hpy166II GTNNAC 5 cut(s) 65, 274, 1255, 2447, 2547
Hpy8I GTNNAC 5 cut(s) 65, 274, 1255, 2447, 2547
HpyCH4III ACNGT 4 cut(s) 775, 1429, 2984, 3069
HpyCH4IV ACGT 2 cut(s) 3139, 3478
HpyF10VI GCNNNNNNNGC 9 cut(s) 515, 544, 842, 1325, 1334, 2285, 2498, 3320, 3506
HpySE526I ACGT 2 cut(s) 3139, 3478
Hsp92I GRCGYC 1 cut(s) 1990
Hsp92II CATG 9 cut(s) 324, 353, 1694, 1700, 1789, 1867, 2348, 3062, 3245
HspAI GCGC 3 cut(s) 101, 1990, 2499
KasI GGCGCC 1 cut(s) 1989
KspAI GTTAAC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 3516
LmnI GCTCC 5 cut(s) 535, 706, 1886, 2317, 3559
Lsp1109I GCAGC 9 cut(s) 69, 166, 1093, 1165, 1357, 2272, 3031, 3067, 3141
MaeII ACGT 2 cut(s) 3139, 3478
MaeIII GTNAC 9 cut(s) 424, 664, 743, 1141, 1303, 1747, 2164, 3131, 3401
MfeI CAATTG 1 cut(s) 3324
MflI RGATCY 5 cut(s) 736, 2576, 2651, 2665, 2762
MhlI GDGCHC 2 cut(s) 1582, 1891
MlsI TGGCCA 1 cut(s) 3203
MluNI TGGCCA 1 cut(s) 3203
Mly113I GGCGCC 1 cut(s) 1990
MlyI GAGTC 7 cut(s) 629, 775, 1004, 1195, 1400, 1670, 3131
MmeI TCCRAC 8 cut(s) 540, 1784, 1796, 1894, 2067, 2409, 2940, 3149
Mox20I TGGCCA 1 cut(s) 3203
Mph1103I ATGCAT 1 cut(s) 2350
MroXI GAANNNNTTC 1 cut(s) 379
MscI TGGCCA 1 cut(s) 3203
MslI CAYNNNNRTG 2 cut(s) 1695, 1701
Msp20I TGGCCA 1 cut(s) 3203
MspA1I CMGCKG 4 cut(s) 60, 704, 892, 3044
MspCI CTTAAG 2 cut(s) 571, 3316
MspI CCGG 3 cut(s) 31, 1910, 3018
MspR9I CCNGG 5 cut(s) 1910, 1994, 2466, 2574, 3363
MunI CAATTG 1 cut(s) 3324
Mva1269I GAATGC 2 cut(s) 2236, 3376
MvaI CCWGG 4 cut(s) 1994, 2466, 2574, 3363
MvnI CGCG 1 cut(s) 2934
MwoI GCNNNNNNNGC 9 cut(s) 515, 544, 842, 1325, 1334, 2285, 2498, 3320, 3506
NarI GGCGCC 1 cut(s) 1990
NciI CCSGG 1 cut(s) 1910
NlaIII CATG 9 cut(s) 324, 353, 1694, 1700, 1789, 1867, 2348, 3062, 3245
NlaIV GGNNCC 6 cut(s) 1164, 1376, 1991, 2187, 2463, 3555
NmeAIII GCCGAG 2 cut(s) 3520, 3530
NmuCI GTSAC 5 cut(s) 664, 743, 1141, 3131, 3401
NsiI ATGCAT 1 cut(s) 2350
NspI RCATGY 2 cut(s) 1700, 1867
NspV TTCGAA 1 cut(s) 3514
PaeI GCATGC 1 cut(s) 1700
PagI TCATGA 1 cut(s) 3241
PaqCI CACCTGC 1 cut(s) 2524
PceI AGGCCT 1 cut(s) 3500
PciSI GCTCTTC 1 cut(s) 3516
PctI GAATGC 2 cut(s) 2236, 3376
PdmI GAANNNNTTC 1 cut(s) 379
PfoI TCCNGGA 2 cut(s) 2572, 3361
PleI GAGTC 7 cut(s) 629, 774, 1003, 1195, 1399, 1670, 3130
PluTI GGCGCC 1 cut(s) 1993
PpsI GAGTC 7 cut(s) 629, 774, 1003, 1195, 1399, 1670, 3130
PpuMI RGGWCCY 4 cut(s) 1162, 1375, 2185, 3359
PshBI ATTAAT 2 cut(s) 1101, 2694
Psp5II RGGWCCY 4 cut(s) 1162, 1375, 2185, 3359
Psp6I CCWGG 4 cut(s) 1992, 2464, 2572, 3361
PspFI CCCAGC 1 cut(s) 2188
PspGI CCWGG 4 cut(s) 1992, 2464, 2572, 3361
PspN4I GGNNCC 6 cut(s) 1164, 1376, 1991, 2187, 2463, 3555
PspPI GGNCC 9 cut(s) 619, 1162, 1375, 1573, 2185, 2462, 3076, 3359, 3519
PspPPI RGGWCCY 4 cut(s) 1162, 1375, 2185, 3359
PsrI GAACNNNNNNTAC 2 cut(s) 2397, 2429
PstNI CAGNNNCTG 2 cut(s) 1844, 2579
PsuI RGATCY 5 cut(s) 736, 2576, 2651, 2665, 2762
PvuII CAGCTG 3 cut(s) 60, 892, 3044
RsaI GTAC 4 cut(s) 5, 64, 2406, 3210
RsaNI GTAC 4 cut(s) 4, 63, 2405, 3209
RseI CAYNNNNRTG 2 cut(s) 1695, 1701
SapI GCTCTTC 1 cut(s) 3516
Sau96I GGNCC 9 cut(s) 619, 1162, 1375, 1573, 2185, 2462, 3076, 3359, 3519
SchI GAGTC 7 cut(s) 629, 775, 1004, 1195, 1400, 1670, 3131
ScrFI CCNGG 5 cut(s) 1910, 1994, 2466, 2574, 3363
SduI GDGCHC 2 cut(s) 1582, 1891
SfcI CTRYAG 2 cut(s) 2241, 2808
SfoI GGCGCC 1 cut(s) 1991
SfuI TTCGAA 1 cut(s) 3514
SinI GGWCC 5 cut(s) 1162, 1375, 2185, 2462, 3359
SmiMI CAYNNNNRTG 2 cut(s) 1695, 1701
SmlI CTYRAG 9 cut(s) 539, 571, 1203, 1640, 2493, 2972, 3212, 3316, 3354
SmoI CTYRAG 9 cut(s) 539, 571, 1203, 1640, 2493, 2972, 3212, 3316, 3354
SpeI ACTAGT 3 cut(s) 839, 2999, 3251
SphI GCATGC 1 cut(s) 1700
SseBI AGGCCT 1 cut(s) 3500
SsiI CCGC 9 cut(s) 109, 148, 356, 704, 2057, 2157, 2932, 3182, 3275
SspDI GGCGCC 1 cut(s) 1989
SspI AATATT 1 cut(s) 1486
StuI AGGCCT 1 cut(s) 3500
StyD4I CCNGG 5 cut(s) 1908, 1992, 2464, 2572, 3361
StyI CCWWGG 3 cut(s) 921, 3204, 3501
TaaI ACNGT 4 cut(s) 775, 1429, 2984, 3069
TaiI ACGT 2 cut(s) 3142, 3481
TaqI TCGA 4 cut(s) 153, 334, 3005, 3514
TatI WGTACW 2 cut(s) 3, 62
TauI GCSGC 1 cut(s) 3278
TscAI CASTG 8 cut(s) 319, 681, 1023, 2008, 2140, 2208, 3270, 3394
TseFI GTSAC 5 cut(s) 664, 743, 1141, 3131, 3401
TseI GCWGC 9 cut(s) 57, 179, 1106, 1178, 1345, 2285, 3044, 3055, 3154
Tsp45I GTSAC 5 cut(s) 664, 743, 1141, 3131, 3401
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 8 cut(s) 319, 681, 1023, 2008, 2140, 2208, 3270, 3394
Vha464I CTTAAG 2 cut(s) 571, 3316
VpaK11BI GGWCC 5 cut(s) 1162, 1375, 2185, 2462, 3359
VspI ATTAAT 2 cut(s) 1101, 2694
XapI RAATTY 8 cut(s) 14, 196, 477, 1278, 1795, 1854, 1941, 3544
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 2 cut(s) 1700, 1867
XcmI CCANNNNNNNNNTGG 2 cut(s) 1336, 3056
XmnI GAANNNNTTC 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.