pycom05g30660

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011014
Physical Location & Seq
Reverse (-)
30704627 .. 30707311
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30660.1

Sequence Viewer

Length: 2685 bp
ATGATGACAAACAGGAAGAATTTTCAATTCAGTATGAGCAGGAGGATGGCTTTAATTTTGATCATCTTATTAGTGCTTCTGATCGATCTGGATGGTCATTTCGAAGTGACAGATGAGGATATGGAGGACCCAGAAATTGCTGCTGCTTTACAATCTTTAGGATGGTCTCAAGATTCTAATAATGCGGAAACTTCTCCCCAGATTGCTGCCGTTGACAGGGAAGCTCTATTAAGCGAAATTCAATCATTAAAAAGAGAGGCAGTTAATCATAAACGGGCAGGTAATGTTCAAGAGGCAATGGCGCAGCTAAAGAGGGCAAAACTACTTGAAAGGGACCTTGAAAGCCTTGAGTCTCAAGAGGGCAATGTTGCAAATGATCGTGCGACAATGCATAAACAAACTGCAGATAAGTCGTCCAAGTCATTTATGGTGGATGATGGAAGTATTAGTACAATGGAACGTACGGACTCCAAACCTGCAAGGAAAAGTAAATTAATGATTCAGAAAGAGCTTCTGGGCTTAAAAAAGAAAGCCCTTTCTCTGAGAAGGGAAGGACGATTAAATGAGGCTGACGAAGAATTGAAGAAGGGCATGGTTCTTGAACAGCAGCTAGAAGAGCTAGAGAATGGTTCTGTACAGAAGGCAATGCCTGGGACTGTTGGCAATAAAGTCCCAGATTTGGCACATGAGCTTCCCAATGTCTCTGAAAGTCTGCCAGTTGCAGATGAGGAAGGAGAAAATGTAACAGATCAGGATATGCATGATCCAGCATATCTTTCTATGCTAAAGAATTTGGGTTGGAATGATGATGATAATGAAGGGACCAACTCGTCGTTGGAACCTTCTAAGCAAACGGATAATCTCTCCATGAAGGTCAGTGCACCCTCTGCAACTCCCCCGGCTAATGTCCCAGCTGGAGGATCAAGGAGAAGCAAAGCTGAGATTCAGAGGGAACTCTTAGGGTTGAAAAGGAAAGCTCTTGCTCTGAGGCGCCAGGGAGAGACCGAGGATGCAGAAGAACTGCTAAAAAAGGTGAAAGCACTAGAGGGCCAGATGATGGAGATGGAAGCACCCAAGGAAGATATCACTGAACCTCCTCCTAATAGTGTTGAGGAGGAAGGAGATGGAGGTAATGTAACAGGAAATAGTACACAGGACCCAGCACTTTTCTCAGAGGGAACTTCCTTTTCCAAACCAGCCGTTTCTGCATCAAGAAATAAGGGTGCAATCCAAAGGGAACTTCTGGATTTAAAAAGAAAGGCTCTTGTCTTTAGACGGAAGGGAGAAACCAAAGAAGCTGAGGAAGTTTTGAGAATGGCAAAAGTGCTAGAGATTCAAATTGAAGAGATGGAAGCCCCAAAGGATTTGAGTCTGCATGATGATTCAAGGGAAGAGAAATCTGAGAATTTCGGATTACTAATTAACACTGAAAAGCCAGGGAATTTGAAGCATGATACAGATGTTAGAAGGTTAACTGAGGCGGCAATGGGTCCAATTGACAGAGTAGTTATGTTATCAGCAAGGAATTCTGAGTCTGTTCCTCTAACCTCACAGTTAGCTAAGGGAAACCAACCATTACCAGTGGAATTGGGTGCTTCGGGTGAAAATTATTTTCCAGATGACCAAAGAGTAGCCAAAGGCGTTAGTCAGATTTCTGCACCTGTCCAATCTGGAAACTTAGTGGATTTGTTGACGGGGGATGACTGGAGAAGTTCTCAAAGGCCTGCTGAAAAACAAGATGATGACCTAAAATTTGATTCTGTTGATTCCCTTACTGCCAGTCCTCCCGTTCAGTTGGGATCCCAGACATGTTCGAATGTAAATCTAGGAAGCCAAAATAATAAATTTGATAAACAGGAAGATAAGCGAGATGTTAATGAAGCAAATTCGGTTCAGGAATCTGCTTCTCAGAGCAACCAAAGTGCCATTCGGCAAGAAATCCTGGGTTTCAAGAGGAGAGCATTAGCTTTAAAGAGAGAAGGGAAACTGACTGAAGCTCGCGAGGAACTAAGGCAGGCAAAGTTATTGGAGAAGTGTCTTGATGAAGGTAGTCCTCAGTCCAAAACTACTTCAAGTGAGGCGTCCAGTGCCACACAAAATACAACTGGCGAGCAGAGCCAACCCCAACCATTACAGTCACGAGACATCCCATCTTCTATCCAAAAGCATCACGGTTCACCATCCTCGGACCCAAAACCTTTGTCCAGTCGTGATCGATTCAAGTTGCAACAGGAGTCTCTTGGCCACAAACGTCAGGCTATGAAGTTGCGTAGGGAGGGTCGGATGGAAGAAGCAGAAGCCGAGTTTGAATTAGCCAAGGCACTTGAGAATCAGTTGGAGGCGTCAGCTGCTCATGATTCCACAACCGTTGACAAAGTAGAATCAGTGGATAATGTCTCTGTTGAGGGGCTTCTTGATCCTCAACTACTGGCTGCCTTGAAAGAAATTGGAATTGAAAGTGCTACTAATTTATCTAAAGGCCCGGAAAGACCAGAGCCTTCAAAAGTTAATGTTGGCAAGAACAGTAACATAATCCAAGATAGAAGTCAGCTGGAAGAACAGATCAAGGCTGAGAAGGTAAAGGCGGTAAATTTGAAACGTGCTGGAAAACAAGCTGAGGCCTTGGATGCTCTTCGAAAGGCCAAGCTACTCGAAAAGAAGCTCAATTCTCCCTCCTCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006897 GO:0007034 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009057 GO:0009653 GO:0009987 GO:0010927 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030163 GO:0030435 GO:0030437 GO:0031090 GO:0031321 GO:0031410 GO:0031982 GO:0032266 GO:0032502 GO:0032505 GO:0032509 GO:0032511 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033565 GO:0034293 GO:0034613 GO:0035091 GO:0036211 GO:0036452 GO:0042763 GO:0042764 GO:0042886 GO:0043162 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043328 GO:0043412 GO:0043632 GO:0043934 GO:0043935 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045184 GO:0045324 GO:0046907 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051321 GO:0051603 GO:0051641 GO:0051649 GO:0051704 GO:0061659 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0120113 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901981 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE

Protein Analysis

895

Amino Acids

98.43

Weight (kDa)

5.24

Isoelectric Point (pI)

58.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 829
Acc36I ACCTGC 2 cut(s) 269, 484
AccB1I GGYRCC 1 cut(s) 990
AccB7I CCANNNNNTGG 1 cut(s) 1057
AccII CGCG 1 cut(s) 2001
AciI CCGC 3 cut(s) 185, 1481, 2585
AclWI GGATC 5 cut(s) 758, 928, 1794, 1807, 2411
AcoI YGGCCR 1 cut(s) 2242
AcuI CTGAAG 1 cut(s) 2013
AcyI GRCGYC 3 cut(s) 991, 2081, 2342
AfaI GTAC 4 cut(s) 451, 463, 636, 1150
AfiI CCNNNNNNNGG 4 cut(s) 216, 679, 917, 1057
AflIII ACRYGT 1 cut(s) 1808
AjnI CCWGG 4 cut(s) 649, 993, 1435, 1941
AjuI GAANNNNNNNTTGG 4 cut(s) 1224, 1256, 1911, 1943
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 7 cut(s) 171, 357, 706, 995, 2136, 2241, 2401
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 5 cut(s) 758, 928, 1794, 1807, 2411
AoxI GGCC 6 cut(s) 1048, 1721, 2242, 2479, 2619, 2640
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 7 cut(s) 140, 143, 206, 304, 607, 2348, 2432
ArsI GACNNNNNNTTYG 4 cut(s) 1736, 1768, 2186, 2218
AseI ATTAAT 1 cut(s) 494
AspLEI GCGC 2 cut(s) 304, 993
AspS9I GGNCC 8 cut(s) 127, 334, 822, 1048, 1156, 1490, 2188, 2480
AsuC2I CCSGG 2 cut(s) 899, 2483
AsuHPI GGTGA 3 cut(s) 1045, 1613, 2169
AsuII TTCGAA 3 cut(s) 102, 1814, 2635
AvaII GGWCC 6 cut(s) 127, 334, 822, 1156, 1490, 2188
BaeGI GKGCMC 1 cut(s) 883
BaeI ACNNNNGTAYC 2 cut(s) 1446, 1479
BalI TGGCCA 1 cut(s) 2244
BamHI GGATCC 1 cut(s) 1799
BanI GGYRCC 1 cut(s) 990
BarI GAAGNNNNNNTAC 2 cut(s) 433, 465
BauI CACGAG 1 cut(s) 2139
Bbv12I GWGCWC 1 cut(s) 883
BbvCI CCTCAGC 2 cut(s) 1299, 2616
BbvI GCAGC 7 cut(s) 127, 130, 193, 316, 619, 2335, 2419
BceAI ACGGC 2 cut(s) 194, 1184
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 4 cut(s) 651, 995, 1437, 1943
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 2 cut(s) 899, 2483
BcoDI GTCTC 7 cut(s) 171, 357, 706, 995, 2136, 2241, 2401
BfaI CTAG 5 cut(s) 611, 620, 1043, 1328, 1826
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 994
BfuAI ACCTGC 2 cut(s) 269, 484
BisI GCNGC 8 cut(s) 141, 144, 207, 305, 608, 1482, 2349, 2433
BlsI GCNGC 8 cut(s) 142, 145, 208, 306, 609, 1483, 2350, 2434
Bme1390I CCNGG 6 cut(s) 651, 899, 995, 1437, 1943, 2483
Bme18I GGWCC 6 cut(s) 127, 334, 822, 1156, 1490, 2188
BmgT120I GGNCC 8 cut(s) 127, 334, 822, 1048, 1156, 1490, 2188, 2480
BmiI GGNNCC 9 cut(s) 129, 335, 823, 840, 992, 1158, 1491, 1801, 2190
BmrFI CCNGG 6 cut(s) 651, 899, 995, 1437, 1943, 2483
BmsI GCATC 4 cut(s) 1000, 1217, 2176, 2617
BplI GAGNNNNNCTC 2 cut(s) 1699, 1731
BpmI CTGGAG 2 cut(s) 936, 1726
Bpu10I CCTNAGC 3 cut(s) 1299, 1560, 2616
Bpu14I TTCGAA 3 cut(s) 102, 1814, 2635
BpuEI CTTGAG 4 cut(s) 153, 339, 368, 2345
BpuMI CCSGG 2 cut(s) 899, 2483
Bsa29I ATCGAT 2 cut(s) 84, 2215
BsaHI GRCGYC 3 cut(s) 991, 2081, 2342
BsaI GGTCTC 2 cut(s) 171, 995
BsaXI ACNNNNNCTCC 6 cut(s) 726, 756, 1078, 1108, 1119, 1149
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 679, 917, 1057
Bse1I ACTGG 8 cut(s) 716, 1580, 1709, 1779, 2085, 2110, 2205, 2433
Bse3DI GCAATG 4 cut(s) 303, 370, 651, 1491
BseBI CCWGG 4 cut(s) 651, 995, 1437, 1943
BseCI ATCGAT 2 cut(s) 84, 2215
BseLI CCNNNNNNNGG 4 cut(s) 216, 679, 917, 1057
BseMI GCAATG 4 cut(s) 303, 370, 651, 1491
BseNI ACTGG 8 cut(s) 716, 1580, 1709, 1779, 2085, 2110, 2205, 2433
BseRI GAGGAG 4 cut(s) 1086, 1127, 1969, 2665
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 7 cut(s) 127, 130, 193, 316, 619, 2335, 2419
BseYI CCCAGC 2 cut(s) 910, 1159
BsgI GTGCAG 1 cut(s) 1641
Bsh1236I CGCG 1 cut(s) 2001
BshFI GGCC 6 cut(s) 1050, 1723, 2244, 2481, 2621, 2642
BshNI GGYRCC 1 cut(s) 990
BshVI ATCGAT 2 cut(s) 84, 2215
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 2 cut(s) 899, 2483
BsiWI CGTACG 1 cut(s) 461
BslFI GGGAC 5 cut(s) 347, 656, 667, 835, 893
BslI CCNNNNNNNGG 4 cut(s) 216, 679, 917, 1057
BsmAI GTCTC 7 cut(s) 171, 357, 706, 995, 2136, 2241, 2401
BsmFI GGGAC 5 cut(s) 347, 656, 667, 835, 893
BsnI GGCC 6 cut(s) 1050, 1723, 2244, 2481, 2621, 2642
Bso31I GGTCTC 2 cut(s) 171, 995
Bsp119I TTCGAA 3 cut(s) 102, 1814, 2635
Bsp1286I GDGCHC 1 cut(s) 883
Bsp1407I TGTACA 1 cut(s) 634
Bsp68I TCGCGA 1 cut(s) 2001
BspACI CCGC 3 cut(s) 185, 1481, 2585
BspANI GGCC 6 cut(s) 1050, 1723, 2244, 2481, 2621, 2642
BspDI ATCGAT 2 cut(s) 84, 2215
BspFNI CGCG 1 cut(s) 2001
BspHI TCATGA 1 cut(s) 2353
BspLI GGNNCC 9 cut(s) 129, 335, 823, 840, 992, 1158, 1491, 1801, 2190
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 2 cut(s) 269, 484
BspPI GGATC 5 cut(s) 758, 928, 1794, 1807, 2411
BspQI GCTCTTC 2 cut(s) 609, 2637
BspT104I TTCGAA 3 cut(s) 102, 1814, 2635
BspT107I GGYRCC 1 cut(s) 990
BspTNI GGTCTC 2 cut(s) 171, 995
BsrDI GCAATG 4 cut(s) 303, 370, 651, 1491
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 8 cut(s) 716, 1580, 1709, 1779, 2085, 2110, 2205, 2433
BssNI GRCGYC 3 cut(s) 991, 2081, 2342
BssSI CACGAG 1 cut(s) 2139
BssT1I CCWWGG 3 cut(s) 1074, 2316, 2622
Bst2BI CACGAG 1 cut(s) 2139
Bst2UI CCWGG 4 cut(s) 651, 995, 1437, 1943
Bst4CI ACNGT 6 cut(s) 658, 1554, 2136, 2174, 2368, 2525
Bst6I CTCTTC 4 cut(s) 609, 1338, 1386, 2637
BstACI GRCGYC 3 cut(s) 991, 2081, 2342
BstAPI GCANNNNNTGC 1 cut(s) 887
BstAUI TGTACA 1 cut(s) 634
BstBI TTCGAA 3 cut(s) 102, 1814, 2635
BstC8I GCNNGC 4 cut(s) 1725, 1999, 2016, 2111
BstFNI CGCG 1 cut(s) 2001
BstH2I RGCGCY 1 cut(s) 994
BstHHI GCGC 2 cut(s) 304, 993
BstMAI GTCTC 7 cut(s) 171, 357, 706, 995, 2136, 2241, 2401
BstMWI GCNNNNNNNGC 7 cut(s) 616, 887, 1205, 2087, 2115, 2348, 2627
BstNI CCWGG 4 cut(s) 651, 995, 1437, 1943
BstNSI RCATGY 1 cut(s) 1812
BstSCI CCNGG 6 cut(s) 649, 897, 993, 1435, 1941, 2481
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 883
BstUI CGCG 1 cut(s) 2001
BstV1I GCAGC 7 cut(s) 127, 130, 193, 316, 619, 2335, 2419
BstX2I RGATCY 1 cut(s) 1799
BstYI RGATCY 1 cut(s) 1799
Bsu15I ATCGAT 2 cut(s) 84, 2215
BsuRI GGCC 6 cut(s) 1050, 1723, 2244, 2481, 2621, 2642
BsuTUI ATCGAT 2 cut(s) 84, 2215
BtsIMutI CAGTG 6 cut(s) 883, 1086, 1425, 1587, 2092, 2391
BtuMI TCGCGA 1 cut(s) 2001
BveI ACCTGC 2 cut(s) 269, 484
Cac8I GCNNGC 4 cut(s) 1725, 1999, 2016, 2111
CciI TCATGA 1 cut(s) 2353
CfoI GCGC 2 cut(s) 304, 993
Cfr13I GGNCC 8 cut(s) 127, 334, 822, 1048, 1156, 1490, 2188, 2480
ClaI ATCGAT 2 cut(s) 84, 2215
CseI GACGC 2 cut(s) 2070, 2331
Csp6I GTAC 4 cut(s) 450, 462, 635, 1149
CspCI CAANNNNNGTGG 2 cut(s) 2350, 2385
CviAII CATG 8 cut(s) 592, 686, 761, 868, 1376, 1451, 1809, 2354
CviQI GTAC 4 cut(s) 450, 462, 635, 1149
DinI GGCGCC 1 cut(s) 992
DraI TTTAAA 2 cut(s) 1251, 1971
DrdI GACNNNNNNGTC 1 cut(s) 829
DseDI GACNNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 1 cut(s) 2242
Eam1104I CTCTTC 4 cut(s) 609, 1338, 1386, 2637
EarI CTCTTC 4 cut(s) 609, 1338, 1386, 2637
Eco130I CCWWGG 3 cut(s) 1074, 2316, 2622
Eco147I AGGCCT 2 cut(s) 1723, 2621
Eco31I GGTCTC 2 cut(s) 171, 995
Eco32I GATATC 1 cut(s) 1084
Eco47I GGWCC 6 cut(s) 127, 334, 822, 1156, 1490, 2188
Eco57I CTGAAG 1 cut(s) 2013
EcoO109I RGGNCCY 3 cut(s) 127, 334, 1156
EcoRI GAATTC 1 cut(s) 1525
EcoRII CCWGG 4 cut(s) 649, 993, 1435, 1941
EcoRV GATATC 1 cut(s) 1084
EcoT14I CCWWGG 3 cut(s) 1074, 2316, 2622
EcoT22I ATGCAT 2 cut(s) 393, 762
EgeI GGCGCC 1 cut(s) 992
EheI GGCGCC 1 cut(s) 992
ErhI CCWWGG 3 cut(s) 1074, 2316, 2622
FaeI CATG 8 cut(s) 595, 689, 764, 871, 1379, 1454, 1812, 2357
FaqI GGGAC 5 cut(s) 347, 656, 667, 835, 893
FatI CATG 8 cut(s) 591, 685, 760, 867, 1375, 1450, 1808, 2353
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 8 cut(s) 141, 144, 207, 305, 608, 1482, 2349, 2433
Fsp4HI GCNGC 8 cut(s) 141, 144, 207, 305, 608, 1482, 2349, 2433
FspBI CTAG 5 cut(s) 611, 620, 1043, 1328, 1826
GlaI GCGC 2 cut(s) 303, 992
GluI GCNGC 8 cut(s) 141, 144, 207, 305, 608, 1482, 2349, 2433
GsaI CCCAGC 2 cut(s) 914, 1163
GsuI CTGGAG 2 cut(s) 936, 1726
HaeII RGCGCY 1 cut(s) 994
HaeIII GGCC 6 cut(s) 1050, 1723, 2244, 2481, 2621, 2642
HapII CCGG 2 cut(s) 899, 2483
HgaI GACGC 2 cut(s) 2070, 2331
HhaI GCGC 2 cut(s) 304, 993
Hin1I GRCGYC 3 cut(s) 991, 2081, 2342
Hin1II CATG 8 cut(s) 595, 689, 764, 871, 1379, 1454, 1812, 2357
Hin6I GCGC 2 cut(s) 302, 991
HinP1I GCGC 2 cut(s) 302, 991
HincII GTYRAC 4 cut(s) 214, 1473, 1692, 2371
HindII GTYRAC 4 cut(s) 214, 1473, 1692, 2371
HpaI GTTAAC 1 cut(s) 1473
HpaII CCGG 2 cut(s) 899, 2483
HphI GGTGA 3 cut(s) 1045, 1613, 2169
Hpy166II GTNNAC 7 cut(s) 214, 881, 1151, 1473, 1692, 2177, 2371
Hpy8I GTNNAC 7 cut(s) 214, 881, 1151, 1473, 1692, 2177, 2371
Hpy99I CGWCG 1 cut(s) 835
HpyCH4III ACNGT 6 cut(s) 658, 1554, 2136, 2174, 2368, 2525
HpyCH4IV ACGT 3 cut(s) 460, 2251, 2599
HpyF10VI GCNNNNNNNGC 7 cut(s) 616, 887, 1205, 2087, 2115, 2348, 2627
HpySE526I ACGT 3 cut(s) 460, 2251, 2599
Hsp92I GRCGYC 3 cut(s) 991, 2081, 2342
Hsp92II CATG 8 cut(s) 595, 689, 764, 871, 1379, 1454, 1812, 2357
HspAI GCGC 2 cut(s) 302, 991
KasI GGCGCC 1 cut(s) 990
Ksp22I TGATCA 1 cut(s) 60
KspAI GTTAAC 1 cut(s) 1473
LguI GCTCTTC 2 cut(s) 609, 2637
Lsp1109I GCAGC 7 cut(s) 127, 130, 193, 316, 619, 2335, 2419
LweI GCATC 4 cut(s) 1000, 1217, 2176, 2617
MaeI CTAG 5 cut(s) 611, 620, 1043, 1328, 1826
MaeII ACGT 3 cut(s) 460, 2251, 2599
MaeIII GTNAC 5 cut(s) 106, 742, 1135, 2136, 2525
MfeI CAATTG 1 cut(s) 1494
MflI RGATCY 1 cut(s) 1799
MhlI GDGCHC 1 cut(s) 883
MlsI TGGCCA 1 cut(s) 2244
MluNI TGGCCA 1 cut(s) 2244
Mly113I GGCGCC 1 cut(s) 991
MlyI GAGTC 5 cut(s) 359, 461, 1378, 1541, 2243
MmeI TCCRAC 4 cut(s) 779, 816, 2261, 2316
Mox20I TGGCCA 1 cut(s) 2244
Mph1103I ATGCAT 2 cut(s) 393, 762
MscI TGGCCA 1 cut(s) 2244
Msp20I TGGCCA 1 cut(s) 2244
MspA1I CMGCKG 3 cut(s) 914, 2348, 2551
MspI CCGG 2 cut(s) 899, 2483
MspR9I CCNGG 6 cut(s) 651, 899, 995, 1437, 1943, 2483
MunI CAATTG 1 cut(s) 1494
MvaI CCWGG 4 cut(s) 651, 995, 1437, 1943
MvnI CGCG 1 cut(s) 2001
MwoI GCNNNNNNNGC 7 cut(s) 616, 887, 1205, 2087, 2115, 2348, 2627
NarI GGCGCC 1 cut(s) 991
NciI CCSGG 2 cut(s) 899, 2483
NlaIII CATG 8 cut(s) 595, 689, 764, 871, 1379, 1454, 1812, 2357
NlaIV GGNNCC 9 cut(s) 129, 335, 823, 840, 992, 1158, 1491, 1801, 2190
NmeAIII GCCGAG 1 cut(s) 2326
NmuCI GTSAC 2 cut(s) 106, 2136
NruI TCGCGA 1 cut(s) 2001
NsiI ATGCAT 2 cut(s) 393, 762
NspI RCATGY 1 cut(s) 1812
NspV TTCGAA 3 cut(s) 102, 1814, 2635
PagI TCATGA 1 cut(s) 2353
PceI AGGCCT 2 cut(s) 1723, 2621
PciI ACATGT 1 cut(s) 1808
PciSI GCTCTTC 2 cut(s) 609, 2637
Pfl23II CGTACG 1 cut(s) 461
PflMI CCANNNNNTGG 1 cut(s) 1057
PkrI GCNGC 8 cut(s) 142, 145, 208, 306, 609, 1483, 2350, 2434
PleI GAGTC 5 cut(s) 358, 461, 1377, 1540, 2242
PluTI GGCGCC 1 cut(s) 994
PpsI GAGTC 5 cut(s) 358, 461, 1377, 1540, 2242
PpuMI RGGWCCY 3 cut(s) 127, 334, 1156
PscI ACATGT 1 cut(s) 1808
PshBI ATTAAT 1 cut(s) 494
Psp5II RGGWCCY 3 cut(s) 127, 334, 1156
Psp6I CCWGG 4 cut(s) 649, 993, 1435, 1941
PspFI CCCAGC 2 cut(s) 910, 1159
PspGI CCWGG 4 cut(s) 649, 993, 1435, 1941
PspLI CGTACG 1 cut(s) 461
PspN4I GGNNCC 9 cut(s) 129, 335, 823, 840, 992, 1158, 1491, 1801, 2190
PspPI GGNCC 8 cut(s) 127, 334, 822, 1048, 1156, 1490, 2188, 2480
PspPPI RGGWCCY 3 cut(s) 127, 334, 1156
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 1799
PvuII CAGCTG 3 cut(s) 914, 2348, 2551
RruI TCGCGA 1 cut(s) 2001
RsaI GTAC 4 cut(s) 451, 463, 636, 1150
RsaNI GTAC 4 cut(s) 450, 462, 635, 1149
SapI GCTCTTC 2 cut(s) 609, 2637
SatI GCNGC 8 cut(s) 141, 144, 207, 305, 608, 1482, 2349, 2433
Sau96I GGNCC 8 cut(s) 127, 334, 822, 1048, 1156, 1490, 2188, 2480
SchI GAGTC 5 cut(s) 359, 461, 1378, 1541, 2243
ScrFI CCNGG 6 cut(s) 651, 899, 995, 1437, 1943, 2483
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 4 cut(s) 1000, 1217, 2176, 2617
SfcI CTRYAG 1 cut(s) 402
SfoI GGCGCC 1 cut(s) 992
SfuI TTCGAA 3 cut(s) 102, 1814, 2635
SinI GGWCC 6 cut(s) 127, 334, 822, 1156, 1490, 2188
SmlI CTYRAG 4 cut(s) 168, 347, 354, 2324
SmoI CTYRAG 4 cut(s) 168, 347, 354, 2324
SseBI AGGCCT 2 cut(s) 1723, 2621
SsiI CCGC 3 cut(s) 185, 1481, 2585
SspDI GGCGCC 1 cut(s) 990
SspMI CTAG 5 cut(s) 611, 620, 1043, 1328, 1826
StuI AGGCCT 2 cut(s) 1723, 2621
StyD4I CCNGG 6 cut(s) 649, 897, 993, 1435, 1941, 2481
StyI CCWWGG 3 cut(s) 1074, 2316, 2622
TaaI ACNGT 6 cut(s) 658, 1554, 2136, 2174, 2368, 2525
TaiI ACGT 3 cut(s) 463, 2254, 2602
TaqI TCGA 7 cut(s) 84, 102, 1814, 2215, 2635, 2652, 2678
TaqII GACCGA 1 cut(s) 1019
TatI WGTACW 3 cut(s) 449, 634, 1148
TauI GCSGC 1 cut(s) 1484
TscAI CASTG 6 cut(s) 883, 1093, 1432, 1587, 2092, 2391
TseFI GTSAC 2 cut(s) 106, 2136
TseI GCWGC 7 cut(s) 140, 143, 206, 304, 607, 2348, 2432
Tsp45I GTSAC 2 cut(s) 106, 2136
TspDTI ATGAA 5 cut(s) 831, 884, 1893, 2058, 2276
TspGWI ACGGA 3 cut(s) 479, 869, 1291
TspRI CASTG 6 cut(s) 883, 1093, 1432, 1587, 2092, 2391
Van91I CCANNNNNTGG 1 cut(s) 1057
VneI GTGCAC 1 cut(s) 879
VpaK11BI GGWCC 6 cut(s) 127, 334, 822, 1156, 1490, 2188
VspI ATTAAT 1 cut(s) 494
XceI RCATGY 1 cut(s) 1812
XcmI CCANNNNNNNNNTGG 2 cut(s) 424, 832
XspI CTAG 5 cut(s) 611, 620, 1043, 1328, 1826
Zsp2I ATGCAT 2 cut(s) 393, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.