MD10G1310700.v1.1

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011014
Physical Location & Seq
Reverse (-)
39569307 .. 39574323
5017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1310700.v1.1.491

Sequence Viewer

Length: 3801 bp
ATGTTGGAGAAGATCGGGCTGCCGGCGAAGCCGTCACTGAGAGGGAATACTTGGGTGGTTGACTGCTCTCACTGCCAGGGATGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCACCATTGCAGGAGGTGTGGCGGCTTGTTTTGCAACAGTTGCACCCAGCAAAGAATGCTCTTACGCGGGCAAGGTGATTCGCCTGTGCGTATTTGTGACCCTTGTAAAAAGCTAGAAGAAGCAGTACGCTTTGAGAGATATGGACACAAGAGTAGAGCCGGGAGAGGCAGTTCAAAGTTGACATCAAATCATGATGATGAAGTTTTGGACGAGATTCTCGGTAGTGACAGAAAGGAATTAGGACAAGAGTTGAACAGTAATATGGTTTCCAGTATGCAGAGAGCCGCTAGTAGTGCGTCATCTTCAAGTAGTCAACAAAATTCCAGCCACGATGGGGTAGGAGAAATACATAGAAGTCTTTCCGTTGATGAGCCTAATTTACAGAGTGGTGGTGGATCTGCTTCTCCTGATGAATTGCGCCAGCAAGCTTTGGAAGAGAAAAAGAAGTATAAAGTTCTTAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAGAGGGAAGGAGCTTGAGAGACAGGCTGACACATTGGAAACATCTTTGAGAAAAGAGCGTAGAAAGGTTTTACTTTCTGCCAATGTGGTGGAGAGCCAGATTAAAGATGGGCCTTCTGAATCTGGAAGAAGAAATAAGGTCACTCCTCCAGTTGGTAAAGAAAAGGATGACCTTTCTACTGAGCTTAAAGAACTAGGATGGTCGGATATGGATCTCCTTGATGAAAACAAAAAACAAGCAAGTCTGAGTTTGGAGGGCGAACTTTCTTCTCTTCTGGGGGAGGTCTCACAAAAGACCAATAAAAACAAAGGTACTGGTGCCATTGACAAAACCCAGGTTGTTGCACTTAAAAAGAAGGCTCTCATGCTGAAGCGTGAGGGTAAACTTACAGAAGCTAAGGAGGAACTAAAAAGAGCGAAAATTCTAGAGAAGGAACTCGAGGAACAGGAATTCTTGGCTGAGGCTGAAGATTCTGATGATGAGCTATCTGCGTTGATTCGTAGTATGGATGATGACAAACAGGAAGAATTTTCAATTCAATACGAGCAGGAGGATAGCTTCAATTTTGATCATCTTATTAGTGCTGCTGATGATCTGGATGGTAATTTTGAAGTGACAGATGAAGATATGGAGGACCCAGAAATAAGTGCTGCCTTACAATCTTTAGGATGGTCTCAAGATTCTAATAATCCGGAAACTTCTCCCCAGATACCTTCCGTTGACAGGGAAGCTCTATTAAGCGAAATTCAATCATTGAAAAGAGAGGCACTTAATCATAAACGGGCAGGTAATGTGCAACAGGCAATGACACAGCTAAAGAAGGCAAAACTACTTGAACGGGACCTTGAAAGCCTTGAGTCTCAAGAGGGCAATGTTGCAAATGATCCTGCGAGAATTCATAAACAAGCTGCTGATAAATCATTGCAGTCACCTATGGTGGGTGATATTCATACAATGGAACCTACGGACTCCAAACCTGCAAGGAAGACTAAACTAATGATTCAGAAAGAGCTTCTGGGCTTGAAAAAGAAAGCCCTTGCTCTGAGAAGGGAAGGAAGACTAAATGAGGCTGAAGAAGAATTGAAGAAAGGAATGGTACTTGAGCAGGAGCTTGAAGAGCTAGAGAATGGTTCTATGCGGAAGGAAATGCCTGGGACTGTTGGCAGTAAAGTCCCAGTTTTGGCACATGAGCTTCCCAATGTTTCTGCAGGTCTGCCAGTTGCAGATGAGGAAGGAGAAAATGTAACAGATCAAGATATGCATGATCCAGCATACCTTTCTATGTTAAAGAATTTGGGTTGGAATGATGAAGCAAATGAAGGGACCAACTCGTCTTTGGAAACTTCTAAGCGAATGGATAGTCTCTCAATGAAGGTCAGTGAACCCGCTGTAACTCAAGCCGCGGTTAATGTCCCGGCTGGAGGATCAAGGAGAAGCAAAGGTGAAATTCAGAGGGAACTCTTAGGTTTGAAAAGGAAAGCTCTTGCTCTGAGGTGCCAAGGAGAGACTGAGGATGCAGAAGAACTTCTAAAAAAGGCAAAAGCACTAGAGGGCCAGATGTTGGAAATGGAAGCACCCAAGGAAAATATCATTGAACCTCCTCTTAATAGTGCTGAGGAGGAACGTGATAGAGGTGATGTAACAGAAAGTAGTATGCTGGACCCAGCACTTATCTCAGAGGGAACTTACTCATACAAACCAGCCGTTTCTGCACCCAGAAATAAAGGTGCAATCCAAAGGGAACTTTTGGATTTGAAGAGAAAGGCTCTTGCCTTTAGACAGAAGGGAGAAACCAAAGAAGCTGAGGAAGTGTTGAGGATGGCAAAGGTGCTAGAGATTCAAATTGAAGAGATGGAAGCCCCAAGGGATTTGAGTCTGCATGATGATTCAAAGGAAGAGAAATCTGAGAGTTTCGGATATCTAATTAACACCGAAAAGCCAGGGAATTTGAAGGATGATACAGATGTTAGAAGGTTTACTGAGGCAGCAATGGGTCCAATTGACGATGTTGTTAAGTTGTCAGCAAAGAATTCTGAGTTTGTTCCTCTAACCACACAGCTAGCTAAAGGAAGCCAACCATTTCCAGTGGAATTGGGTGCTTTGTGTGAAACTTATTTTCCGGATGACCAAAAAATAGCGGAAGGCTTTAGTCAGATATCTACACCTGTCCAATCTGGAAACTTAGTTGATTTGTTGACAGGGGATGACTGGAGAAGTTATCAAAGGCCAGCTGAAAAACAAGATGATGGCCTAAAGTTTGTTTCTGTTGGTTCCTTTACCGCCAGTCCTCCTGTGCAGTTGGGATCCCAGACCTGTTCGAATGTATATCTGGGAAGCCAAGATGATAAAATTGATAAACAGGAAGATAAGCGAGATGTTAATGTAGCAAATTCAGTTCAGGAAGCTGCTTCTCAGAGCAGCCAAAGTGCCATTCGGCAAGAAATCCTAGCTTTTAAGAGGAGGGCATTAGCTTTAAAGAGAGAAGGGAAACTGACAGAAGCTCGAGAGGAACTTCGTCAGGCAAAGTTATTGGAGAAGCGTCTTGACGAAGATAGTCCTCAGTCCAAAACTACTTCAAGTGAGGTGTCCAGTGCCGTACAAAATACAACTGGAGAGCAGAGCCAATCCCAATCATTACAGTCACGAGACATCCCTTGTTCTAGCCAAAAGCATCACGGTTCACCATCTTCGGACCCAAAACCTTTGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTTGGCCACAAACGTCAGGCTATGAAGTTGCGGAGGGAGGGTCGGATGGAAGAAGCAGAAGCCGAGTTTGAATTAGCCAAGGCACTTGAGAATCAGTTGGATCTATCAGCTGCTCATGATTCCGCAACCGTTGACAAAGGAGAATCAATGGATGATGTCTCTGTTGAGGGTCTTGATCCTCAACTACTGGCTGCCTTGAAAGAAATTGGAATCGAAAATGCTAGTAATTTATCGCAAGGCCCCGAAAGACCAGAGCCTGCAAAAGTTAATGTTGGTAAGAGCAATAACACAATCCAAGATAGAAGTCAGCTGGAAGAACAGATCAAGGCAGAGAAGGTAAAGGCGGTAAATTTGAAACGTGCTGGAAAACAAGGGGAGGCCTTGGATGCTCTTCGAAAGGCCAAGCTACTCGAAAAGAAGCTGAATTCTCCGTCTTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006897 GO:0007034 GO:0007049 GO:0008104 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009057 GO:0009653 GO:0009987 GO:0010927 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030163 GO:0030435 GO:0030437 GO:0031090 GO:0031321 GO:0031410 GO:0031982 GO:0032266 GO:0032502 GO:0032505 GO:0032509 GO:0032511 GO:0032989 GO:0032991 GO:0033036 GO:0033365 GO:0033565 GO:0034293 GO:0034613 GO:0035091 GO:0036211 GO:0036452 GO:0042763 GO:0042764 GO:0042886 GO:0043162 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043328 GO:0043412 GO:0043632 GO:0043934 GO:0043935 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0045184 GO:0045324 GO:0046907 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051321 GO:0051603 GO:0051641 GO:0051649 GO:0051704 GO:0061659 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0120113 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901981 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYVE

Protein Analysis

1267

Amino Acids

139.98

Weight (kDa)

5.26

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 5.4e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1945
Acc36I ACCTGC 3 cut(s) 1391, 1600, 1814
AccB1I GGYRCC 2 cut(s) 932, 2109
AccB7I CCANNNNNTGG 1 cut(s) 2176
AccII CGCG 2 cut(s) 180, 2018
AccIII TCCGGA 2 cut(s) 1306, 2734
AclWI GGATC 9 cut(s) 517, 834, 1493, 1874, 2047, 2913, 2926, 3465, 3527
AcoI YGGCCR 1 cut(s) 3361
AcuI CTGAAG 3 cut(s) 1004, 1101, 1707
AfaI GTAC 4 cut(s) 240, 928, 1713, 3213
AfiI CCNNNNNNNGG 7 cut(s) 448, 767, 1338, 1553, 1795, 2036, 2176
AjnI CCWGG 4 cut(s) 75, 948, 1765, 2554
AjuI GAANNNNNNNTTGG 2 cut(s) 3030, 3062
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
Alw26I GTCTC 9 cut(s) 628, 904, 1293, 1479, 1982, 2114, 3255, 3360, 3520
AlwI GGATC 9 cut(s) 517, 834, 1493, 1874, 2047, 2913, 2926, 3465, 3527
AlwNI CAGNNNCTG 1 cut(s) 3614
Ama87I CYCGRG 2 cut(s) 1052, 3117
Aor13HI TCCGGA 2 cut(s) 1306, 2734
AoxI GGCC 8 cut(s) 725, 2167, 2840, 2863, 3361, 3595, 3735, 3756
ApeKI GCWGC 9 cut(s) 19, 1199, 1265, 1523, 2600, 3020, 3033, 3467, 3548
ArsI GACNNNNNNTTYG 4 cut(s) 1960, 1992, 3305, 3337
Asp700I GAANNNNTTC 2 cut(s) 472, 2139
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 9 cut(s) 725, 1249, 1456, 1938, 2167, 2275, 2609, 3307, 3596
AsuC2I CCSGG 2 cut(s) 274, 2030
AsuHPI GGTGA 7 cut(s) 88, 200, 1536, 1568, 2069, 2261, 3288
AsuII TTCGAA 2 cut(s) 2933, 3751
AsuNHI GCTAGC 1 cut(s) 2674
AvaI CYCGRG 2 cut(s) 1052, 3117
AvaII GGWCC 6 cut(s) 1249, 1456, 1938, 2275, 2609, 3307
BaeI ACNNNNGTAYC 2 cut(s) 2565, 2598
BalI TGGCCA 1 cut(s) 3363
BamHI GGATCC 1 cut(s) 2918
BanI GGYRCC 2 cut(s) 932, 2109
BarI GAAGNNNNNNTAC 2 cut(s) 222, 254
BauI CACGAG 1 cut(s) 3258
BbsI GAAGAC 3 cut(s) 1607, 1678, 3783
BbvCI CCTCAGC 3 cut(s) 1074, 2229, 2418
BbvI GCAGC 9 cut(s) 6, 1186, 1252, 1510, 2612, 3007, 3045, 3454, 3535
BceAI ACGGC 3 cut(s) 16, 2303, 3194
BciT130I CCWGG 4 cut(s) 77, 950, 1767, 2556
BclI TGATCA 1 cut(s) 1183
BcnI CCSGG 2 cut(s) 274, 2030
BcoDI GTCTC 9 cut(s) 628, 904, 1293, 1479, 1982, 2114, 3255, 3360, 3520
BfmI CTRYAG 1 cut(s) 1821
BfuAI ACCTGC 3 cut(s) 1391, 1600, 1814
Bme1390I CCNGG 6 cut(s) 77, 274, 950, 1767, 2030, 2556
Bme18I GGWCC 6 cut(s) 1249, 1456, 1938, 2275, 2609, 3307
BmeT110I CYCGRG 2 cut(s) 1052, 3117
BmgT120I GGNCC 9 cut(s) 725, 1249, 1456, 1938, 2167, 2275, 2609, 3307, 3596
BmrFI CCNGG 6 cut(s) 77, 274, 950, 1767, 2030, 2556
BmrI ACTGGG 1 cut(s) 1784
BmsI GCATC 3 cut(s) 2119, 3295, 3733
BmtI GCTAGC 1 cut(s) 2678
BmuI ACTGGG 1 cut(s) 1784
BpiI GAAGAC 3 cut(s) 1607, 1678, 3783
BplI GAGNNNNNCTC 2 cut(s) 2365, 2397
BpmI CTGGAG 4 cut(s) 747, 2055, 2845, 3246
Bpu10I CCTNAGC 4 cut(s) 1011, 1074, 2229, 2418
Bpu14I TTCGAA 2 cut(s) 2933, 3751
BpuEI CTTGAG 7 cut(s) 650, 1275, 1461, 1490, 1736, 1995, 3464
BpuMI CCSGG 2 cut(s) 274, 2030
BsaBI GATNNNNATC 1 cut(s) 825
BsaI GGTCTC 2 cut(s) 904, 1293
BsaWI WCCGGW 2 cut(s) 1306, 2734
BsaXI ACNNNNNCTCC 6 cut(s) 268, 298, 1842, 1872, 3382, 3412
Bsc4I CCNNNNNNNGG 7 cut(s) 448, 767, 1338, 1553, 1795, 2036, 2176
Bse118I RCCGGY 1 cut(s) 22
Bse3DI GCAATG 5 cut(s) 118, 1425, 1492, 1535, 2610
Bse8I GATNNNNATC 1 cut(s) 825
BseAI TCCGGA 2 cut(s) 1306, 2734
BseBI CCWGG 4 cut(s) 77, 950, 1767, 2556
BseJI GATNNNNATC 1 cut(s) 825
BseLI CCNNNNNNNGG 7 cut(s) 448, 767, 1338, 1553, 1795, 2036, 2176
BseMI GCAATG 5 cut(s) 118, 1425, 1492, 1535, 2610
BseRI GAGGAG 4 cut(s) 750, 2205, 2246, 3088
BseXI GCAGC 9 cut(s) 6, 1186, 1252, 1510, 2612, 3007, 3045, 3454, 3535
BseYI CCCAGC 2 cut(s) 159, 2278
BsgI GTGCAG 2 cut(s) 2310, 2930
Bsh1236I CGCG 2 cut(s) 180, 2018
BshFI GGCC 8 cut(s) 727, 2169, 2842, 2865, 3363, 3597, 3737, 3758
BshNI GGYRCC 2 cut(s) 932, 2109
BsiHKCI CYCGRG 2 cut(s) 1052, 3117
BsiSI CCGG 5 cut(s) 23, 273, 1307, 2030, 2735
BslFI GGGAC 5 cut(s) 1469, 1772, 1783, 1951, 2012
BslI CCNNNNNNNGG 7 cut(s) 448, 767, 1338, 1553, 1795, 2036, 2176
BsmAI GTCTC 9 cut(s) 628, 904, 1293, 1479, 1982, 2114, 3255, 3360, 3520
BsmFI GGGAC 5 cut(s) 1469, 1772, 1783, 1951, 2012
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 8 cut(s) 727, 2169, 2842, 2865, 3363, 3597, 3737, 3758
Bso31I GGTCTC 2 cut(s) 904, 1293
BsoBI CYCGRG 2 cut(s) 1052, 3117
Bsp119I TTCGAA 2 cut(s) 2933, 3751
Bsp13I TCCGGA 2 cut(s) 1306, 2734
BspANI GGCC 8 cut(s) 727, 2169, 2842, 2865, 3363, 3597, 3737, 3758
BspEI TCCGGA 2 cut(s) 1306, 2734
BspFNI CGCG 2 cut(s) 180, 2018
BspHI TCATGA 2 cut(s) 304, 3472
BspMAI CTGCAG 1 cut(s) 1825
BspMI ACCTGC 3 cut(s) 1391, 1600, 1814
BspOI GCTAGC 1 cut(s) 2678
BspPI GGATC 9 cut(s) 517, 834, 1493, 1874, 2047, 2913, 2926, 3465, 3527
BspQI GCTCTTC 2 cut(s) 1725, 3753
BspT104I TTCGAA 2 cut(s) 2933, 3751
BspT107I GGYRCC 2 cut(s) 932, 2109
BspTNI GGTCTC 2 cut(s) 904, 1293
BsrDI GCAATG 5 cut(s) 118, 1425, 1492, 1535, 2610
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssSI CACGAG 1 cut(s) 3258
BssT1I CCWWGG 5 cut(s) 2113, 2193, 2477, 3435, 3738
Bst2BI CACGAG 1 cut(s) 3258
Bst2UI CCWGG 4 cut(s) 77, 950, 1767, 2556
Bst4CI ACNGT 6 cut(s) 152, 371, 1774, 3255, 3293, 3487
Bst6I CTCTTC 7 cut(s) 543, 891, 1725, 2366, 2457, 2505, 3753
BstAPI GCANNNNNTGC 2 cut(s) 153, 169
BstBI TTCGAA 2 cut(s) 2933, 3751
BstC8I GCNNGC 8 cut(s) 24, 113, 182, 536, 540, 2676, 2844, 3615
BstDSI CCRYGG 1 cut(s) 2016
BstFNI CGCG 2 cut(s) 180, 2018
BstHHI GCGC 1 cut(s) 534
BstMAI GTCTC 9 cut(s) 628, 904, 1293, 1479, 1982, 2114, 3255, 3360, 3520
BstNI CCWGG 4 cut(s) 77, 950, 1767, 2556
BstSCI CCNGG 6 cut(s) 75, 272, 948, 1765, 2028, 2554
BstSFI CTRYAG 1 cut(s) 1821
BstUI CGCG 2 cut(s) 180, 2018
BstV1I GCAGC 9 cut(s) 6, 1186, 1252, 1510, 2612, 3007, 3045, 3454, 3535
BstV2I GAAGAC 3 cut(s) 1607, 1678, 3783
BstX2I RGATCY 4 cut(s) 509, 826, 2918, 3457
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 4 cut(s) 509, 826, 2918, 3457
BsuRI GGCC 8 cut(s) 727, 2169, 2842, 2865, 3363, 3597, 3737, 3758
BtgI CCRYGG 1 cut(s) 2016
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 5 cut(s) 35, 70, 1999, 2706, 3211
BveI ACCTGC 3 cut(s) 1391, 1600, 1814
Cac8I GCNNGC 8 cut(s) 24, 113, 182, 536, 540, 2676, 2844, 3615
CaiI CAGNNNCTG 1 cut(s) 3614
CciI TCATGA 2 cut(s) 304, 3472
CfoI GCGC 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 9 cut(s) 725, 1249, 1456, 1938, 2167, 2275, 2609, 3307, 3596
Cfr42I CCGCGG 1 cut(s) 2019
CseI GACGC 2 cut(s) 399, 3143
Csp6I GTAC 4 cut(s) 239, 927, 1712, 3212
CviAII CATG 6 cut(s) 305, 979, 1802, 1877, 2495, 3473
CviQI GTAC 4 cut(s) 239, 927, 1712, 3212
DraI TTTAAA 1 cut(s) 3090
DrdI GACNNNNNNGTC 1 cut(s) 1945
DseDI GACNNNNNNGTC 1 cut(s) 1945
EaeI YGGCCR 1 cut(s) 3361
Eam1104I CTCTTC 7 cut(s) 543, 891, 1725, 2366, 2457, 2505, 3753
EarI CTCTTC 7 cut(s) 543, 891, 1725, 2366, 2457, 2505, 3753
Eco130I CCWWGG 5 cut(s) 2113, 2193, 2477, 3435, 3738
Eco147I AGGCCT 1 cut(s) 3737
Eco31I GGTCTC 2 cut(s) 904, 1293
Eco32I GATATC 2 cut(s) 2534, 2772
Eco47I GGWCC 6 cut(s) 1249, 1456, 1938, 2275, 2609, 3307
Eco57I CTGAAG 3 cut(s) 1004, 1101, 1707
Eco88I CYCGRG 2 cut(s) 1052, 3117
EcoO109I RGGNCCY 3 cut(s) 1249, 1456, 3596
EcoRI GAATTC 4 cut(s) 1064, 1509, 2644, 3781
EcoRII CCWGG 4 cut(s) 75, 948, 1765, 2554
EcoRV GATATC 2 cut(s) 2534, 2772
EcoT14I CCWWGG 5 cut(s) 2113, 2193, 2477, 3435, 3738
EcoT22I ATGCAT 1 cut(s) 1878
ErhI CCWWGG 5 cut(s) 2113, 2193, 2477, 3435, 3738
FaeI CATG 6 cut(s) 308, 982, 1805, 1880, 2498, 3476
FaqI GGGAC 5 cut(s) 1469, 1772, 1783, 1951, 2012
FatI CATG 6 cut(s) 304, 978, 1801, 1876, 2494, 3472
FauI CCCGC 2 cut(s) 173, 2008
FbaI TGATCA 1 cut(s) 1183
GlaI GCGC 1 cut(s) 533
GsaI CCCAGC 2 cut(s) 163, 2282
GsuI CTGGAG 4 cut(s) 747, 2055, 2845, 3246
HaeIII GGCC 8 cut(s) 727, 2169, 2842, 2865, 3363, 3597, 3737, 3758
HapII CCGG 5 cut(s) 23, 273, 1307, 2030, 2735
HgaI GACGC 2 cut(s) 399, 3143
HhaI GCGC 1 cut(s) 534
Hin1II CATG 6 cut(s) 308, 982, 1805, 1880, 2498, 3476
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HincII GTYRAC 6 cut(s) 61, 294, 428, 1336, 2811, 3490
HindII GTYRAC 6 cut(s) 61, 294, 428, 1336, 2811, 3490
HindIII AAGCTT 1 cut(s) 540
HpaII CCGG 5 cut(s) 23, 273, 1307, 2030, 2735
HphI GGTGA 7 cut(s) 88, 200, 1536, 1568, 2069, 2261, 3288
HpyCH4III ACNGT 6 cut(s) 152, 371, 1774, 3255, 3293, 3487
HpyCH4IV ACGT 3 cut(s) 2239, 3370, 3715
HpySE526I ACGT 3 cut(s) 2239, 3370, 3715
Hsp92II CATG 6 cut(s) 308, 982, 1805, 1880, 2498, 3476
HspAI GCGC 1 cut(s) 532
Kpn2I TCCGGA 2 cut(s) 1306, 2734
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 1183
KspI CCGCGG 1 cut(s) 2019
LguI GCTCTTC 2 cut(s) 1725, 3753
LmnI GCTCC 2 cut(s) 625, 1723
Lsp1109I GCAGC 9 cut(s) 6, 1186, 1252, 1510, 2612, 3007, 3045, 3454, 3535
LweI GCATC 3 cut(s) 2119, 3295, 3733
MaeII ACGT 3 cut(s) 2239, 3370, 3715
MfeI CAATTG 1 cut(s) 2613
MflI RGATCY 4 cut(s) 509, 826, 2918, 3457
MlsI TGGCCA 1 cut(s) 3363
MluNI TGGCCA 1 cut(s) 3363
MlyI GAGTC 4 cut(s) 1481, 1577, 2497, 3362
MmeI TCCRAC 5 cut(s) 798, 1895, 2157, 3380, 3435
Mox20I TGGCCA 1 cut(s) 3363
Mph1103I ATGCAT 1 cut(s) 1878
MroI TCCGGA 2 cut(s) 1306, 2734
MroNI GCCGGC 1 cut(s) 22
MroXI GAANNNNTTC 2 cut(s) 472, 2139
MscI TGGCCA 1 cut(s) 3363
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 3363
MspA1I CMGCKG 5 cut(s) 2003, 2018, 2846, 3467, 3667
MspI CCGG 5 cut(s) 23, 273, 1307, 2030, 2735
MspR9I CCNGG 6 cut(s) 77, 274, 950, 1767, 2030, 2556
MunI CAATTG 1 cut(s) 2613
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 4 cut(s) 77, 950, 1767, 2556
MvnI CGCG 2 cut(s) 180, 2018
NaeI GCCGGC 1 cut(s) 24
NciI CCSGG 2 cut(s) 274, 2030
NgoMIV GCCGGC 1 cut(s) 22
NheI GCTAGC 1 cut(s) 2674
NlaIII CATG 6 cut(s) 308, 982, 1805, 1880, 2498, 3476
NmeAIII GCCGAG 1 cut(s) 3445
NmuCI GTSAC 7 cut(s) 33, 209, 338, 754, 1228, 1542, 3255
NsiI ATGCAT 1 cut(s) 1878
NspV TTCGAA 2 cut(s) 2933, 3751
PaeR7I CTCGAG 2 cut(s) 1052, 3117
PagI TCATGA 2 cut(s) 304, 3472
PceI AGGCCT 1 cut(s) 3737
PciSI GCTCTTC 2 cut(s) 1725, 3753
PctI GAATGC 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 472, 2139
PflMI CCANNNNNTGG 1 cut(s) 2176
PleI GAGTC 4 cut(s) 1480, 1577, 2496, 3361
PpsI GAGTC 4 cut(s) 1480, 1577, 2496, 3361
PpuMI RGGWCCY 2 cut(s) 1249, 1456
Psp5II RGGWCCY 2 cut(s) 1249, 1456
Psp6I CCWGG 4 cut(s) 75, 948, 1765, 2554
PspFI CCCAGC 2 cut(s) 159, 2278
PspGI CCWGG 4 cut(s) 75, 948, 1765, 2554
PspPI GGNCC 9 cut(s) 725, 1249, 1456, 1938, 2167, 2275, 2609, 3307, 3596
PspPPI RGGWCCY 2 cut(s) 1249, 1456
PspXI VCTCGAGB 1 cut(s) 1052
PstI CTGCAG 1 cut(s) 1825
PstNI CAGNNNCTG 1 cut(s) 3614
PsuI RGATCY 4 cut(s) 509, 826, 2918, 3457
PvuII CAGCTG 3 cut(s) 2846, 3467, 3667
RsaI GTAC 4 cut(s) 240, 928, 1713, 3213
RsaNI GTAC 4 cut(s) 239, 927, 1712, 3212
RseI CAYNNNNRTG 1 cut(s) 309
SacII CCGCGG 1 cut(s) 2019
SapI GCTCTTC 2 cut(s) 1725, 3753
Sau96I GGNCC 9 cut(s) 725, 1249, 1456, 1938, 2167, 2275, 2609, 3307, 3596
SchI GAGTC 4 cut(s) 1481, 1577, 2497, 3362
ScrFI CCNGG 6 cut(s) 77, 274, 950, 1767, 2030, 2556
SfaNI GCATC 3 cut(s) 2119, 3295, 3733
SfcI CTRYAG 1 cut(s) 1821
Sfr274I CTCGAG 2 cut(s) 1052, 3117
Sfr303I CCGCGG 1 cut(s) 2019
SfuI TTCGAA 2 cut(s) 2933, 3751
SgrBI CCGCGG 1 cut(s) 2019
SinI GGWCC 6 cut(s) 1249, 1456, 1938, 2275, 2609, 3307
SlaI CTCGAG 2 cut(s) 1052, 3117
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 9 cut(s) 629, 1052, 1290, 1469, 1476, 1715, 2010, 3117, 3443
SmoI CTYRAG 9 cut(s) 629, 1052, 1290, 1469, 1476, 1715, 2010, 3117, 3443
SseBI AGGCCT 1 cut(s) 3737
StuI AGGCCT 1 cut(s) 3737
StyD4I CCNGG 6 cut(s) 75, 272, 948, 1765, 2028, 2554
StyI CCWWGG 5 cut(s) 2113, 2193, 2477, 3435, 3738
TaaI ACNGT 6 cut(s) 152, 371, 1774, 3255, 3293, 3487
TaiI ACGT 3 cut(s) 2242, 3373, 3718
TaqI TCGA 6 cut(s) 1053, 2933, 3118, 3570, 3751, 3768
TauI GCSGC 3 cut(s) 138, 401, 2018
TscAI CASTG 5 cut(s) 42, 77, 1999, 2706, 3211
TseFI GTSAC 7 cut(s) 33, 209, 338, 754, 1228, 1542, 3255
TseI GCWGC 9 cut(s) 19, 1199, 1265, 1523, 2600, 3020, 3033, 3467, 3548
Tsp45I GTSAC 7 cut(s) 33, 209, 338, 754, 1228, 1542, 3255
TspGWI ACGGA 4 cut(s) 466, 1321, 1595, 3777
TspRI CASTG 5 cut(s) 42, 77, 1999, 2706, 3211
Van91I CCANNNNNTGG 1 cut(s) 2176
VpaK11BI GGWCC 6 cut(s) 1249, 1456, 1938, 2275, 2609, 3307
XbaI TCTAGA 1 cut(s) 1039
XcmI CCANNNNNNNNNTGG 2 cut(s) 719, 1948
XhoI CTCGAG 2 cut(s) 1052, 3117
XmnI GAANNNNTTC 2 cut(s) 472, 2139
Zsp2I ATGCAT 1 cut(s) 1878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.