RchiOBHm_Chr5g0004701

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011014
Physical Location & Seq
Forward (+)
3089821 .. 3094495
4675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28593

Sequence Viewer

Length: 3588 bp
ATGCTGGAGAAGATCGGGTTGCCGGCGAAGCCATCACTGAGAGGGAATAATTGGGTGGTTGACGCCTCACACTGTCAGGGATGTTCTTCTCAGTTCACCTTCATCAATCGCAAGCATCATTGCCGGAGGTGTGGGGGCATTTTCTGTGGCAGCTGTACACAGCAGAGGATGTTTTTGCGTGGGCAAGGCGATGCGCCTGTGCGGATATGTGAGCCTTGTAAGAAGCTAGAAGAAGCAGCGCGGTTCGAGAGATATGGACACAAGAGTAGAGCTGCGAGAGGCAGTTCAAATTTGACATCAAAGCCTGAGGATGAAATTTTGAACCAGATACTTGGTAATGAAGGGAAGGAATCAGGACAAGAGGTTAACAAGAATGTAGTTTCCAGTATGCTGAGGGCAACTAGTAGTGCATCATGTTCAAATAGTCGAGGAGATTCCAGCCATGATGCGGTGGGAGAAATACTTAGTAGTGTTTCTGTTGATAAGTTTAGTCAATTACAGAATGATAGTCAATCTGTTACTCCTGAGGAATTGCGTCAGCAAGCTTTGGATGAAAAAAAGAAGTATAAAATTCTGAAAGGAGAAGGGAAGTCTGCGGAAGCATTAAGAGCCTTTAAGAAAGGGAAGGAGCTTGAGAGGCAGGCTGATGCGTTGGAAATATCCTTAAGAAAAAAGCGTAAAAAGGATTTACTCTCTGACAATGTGGCTGAGGGCCAGACTAAAGATGACTCTTCCCAATCTGGAAGAAGAAATAGGGTCACTCCTCCAGTGGGTAAAGAAAAAGATGACCTTTCAGCGGAGCTTAAAGAATTAGGGTGGTCTGATATGGATCTCCGTGACAAGAATAAAAAACAAGCGAGTCTGACTGTGGAGGGCGAGCTGTCTTCTCTTCTGGGAGAAATCTCACAAAAGAGCAATAAAAGTAAGGCTACTAGTGCCATTGACAAAACACAGGTTGTTGCACATAAAAAGAAGGCTCTCCAGCTGAAGCGTGAGGGCAAGCTTGCAGAAGCCAAGGAAGAATTAAAGAGAGCTAAAGTTCTAGAGAAGCAACTTGAAGAACAGGAATTGTTGGGTGAGGCTGAAGAGTCCGATGATGAAATATCTGCACTGATTCGTAGTATGGATGATGACAAAGAAGATTTTTCAATTCAATATGAGCAGGAGGATGCCTTTAATTTTGATAGTCTTATTAATGCTGCTGATGATCATATTATAGATAGTAATTTTGAAGTGACAGAAGAGGATATGGAGGACCCAGAAATAACTGCTGCCTTAAAATCTTTAGGATGGGCTCAAGATTCAAATAATCCTCCTGAAACCTCTGCTACCCAGATAGCTTCAGTTGACAAGGAAGCACTATTAAGTGAAATTCAATCCTTAAAAAGAGAGGCTGTAACTCTTAAACGAGCAGGTAATGCCACAGAAGCAATGGTGCAGCTAAAAAAGGCAAAACTGCTTGAAAGGGACCTTGAAAGCATTGAGTCCCAAGAGGGCAATGTCGTGAAACCTTCTGCAACAGTTCATAATCAAACTATTGATAGATCATCAAAGTCATCTATGGTGGATGATGGAAATATTAGTGCAATGGAACATATTGACTTCAAACCTGCGAGGAAGAGTAAGTTAATGATTCAAAAAGAGCTTCTGGCTTTGAAGAAGAAGGCCCGTGCTCTGAGACAGGAGGGACGATTAGATGAAGCTGAAGAAGAGTTGAAGAGAGGCAGGATTCTTGAGCATCAGCTTGAAGAAATGGAGAATGGTAAGGACTCAGTTTTGGAACATGAGCATGCCAATGTCTCTGGAAGTCTGTTATTTGCAGATGAGGAAGGAGAGAATGTAACAGATCAAGATATGTATGATCCAACATATCTTTCCATGCTAAGGAATTTGGGTTGGAATGATGATAATGAAGTTGCAAACTCTTCATCAGCACCTGATAAGAAAATAGGTAACATGCAGGCTAGTGAATCTTCTGGAGCACAAGCCCGAAGTGATTTCCCGGTTGGAGGATCAAGGAAAAGTAAATCTGAAATTCAGAAGGAACTATTAGGGTTGAAAAGAAAGGCTCTTGCTCTGAGGCGCCAGGGAGACACCGAGGAAGCAGAAGAAATGCTGAAAAAGGCAAAAGCATTAGAGGGGGAGATGTCGGAGATGGAAGCACCGAAGAAAGTTCAGTCGGACTTTGGTAGGGACAAGGACAATTTCATTGATAATCTTCCTTTCAGTGTAGAGGAAGAAGGTGATGGCGGGGATGTAACAGAAAATGATATGCAGGACCCAGCACTACTCTCAGTGCTAAAGAGTTTGGGGTGGGAGGATAAAGAGCATTCTGTAGAGGCATCTCCTCTGCAATCTTCTTCCAAACTTGCTGTTGCTGCACCAAGAAGTAAGGGTGAAATCCAAAGGGAGCTTTTGGATTTGAAAAGAAAGGCTCATGCATTTAGACGAAAGGGGCAGACTGAAGAAGCTGAAGAAGTGTTGAAGATGGCAAAGGTACTAGAAGTTCAAATTGAAGAGTTGGAAGCCCCAAAGGGTTTACCAATGGAATTGGGTCCAGGTCGCCCAGACAACCAGAGAATTGCTCAAGGCGCTAGTCAGTCTTCACCTGCCCAATCTGGGAACTTCGCAGATTTATTGACGGGGGATGACTGGAAAGGTTCTCCAGGGTCTGCTGAAAAACAATATGATAACCTATCTTATTCTGTTGATTCTGTTAACGCCAGTCCTCCCATTCAGTTGAGATCCTCACAGGAAGATCTGAGAAAAAGAGATGATGCAATAATTAATAAACGGGAAGATACGATTGTAGCCAATGAGAAGCAAGATGCTAAAGACGTCAATTTGGTTCTGGGATCATCTTCACAGAAGAATGAAAGTGCCATTCGGCAAGAAATCGTGGCTTTTAAGAGGAGGGCATTAGCTCTAAAGAGAGAAGGAAAACTGACAGAAGCCCGTGAAGAACTTAGGCAGGCTAAAGTGTTGGAGAAGCGTCTTGAGGAAGACAGTCCTCAGTCCAAAACTAGTTCGAGTGATGTGTCCGGTTCTGCTAACAATGTTCCTCCAGCTGCCCAAAAGCAGCATGGATCACCGTCGTTGGGCCAAAAACCAATGTCCAGTCGTGATCGCTTCAAGTTGCAACAGGAGTCTCTAGGTCACAAACGTCAAGCCCTGAAGCTGCGAAGGGAGGGTCGGATTGAAGAAGCGGAAGCAGAGTTTGAATTGGCCAAGGTACTTGAGGCTCAGTTAGAGGAAAGTGCTACTCATGATTCCACTAGTGTAGCACCAGTGGATGATGCGGCCGTAGAGGGACTTCTTGATCCTGAACTTCTGTCTGCCTTAAGAGCAATTGGAATTGAAGATGCCAATACATCATCTCAAGGTCCTGGAAGACCAGAGCATTCAAAACCCAATGTTGGCAAGAGTGACGATGTAATCCAAGATAGAAGTAATCTGGAGGAACAGATCAAGGCAGAGAAGGTGAAGGCACTAAATCTGAAACGTGCAGGAAAACAAGCCGAGGCCTTGGATGCTCTTCGAAGGGCCAAGATGCTTGAAAAGAAGCTGAATTCCTCGGCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

1195

Amino Acids

132.01

Weight (kDa)

5.43

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FYVE PF01363 21 - 75 1.6e-16 FYVE zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011014)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61690
fragaria_vesca FvH4_3g03041 FvH4_3g03041 FvH4_3g03041
malus_domestica MD05G1335200.v1.1 MD10G1310700.v1.1
prunus_persica Prupe.4G011500_v2.0.a1 Prupe.4G030200_v2.0.a1
pyrus_communis pycom05g30660 pycom10g26170
rosa_chinensis RchiOBHm_Chr5g0004701
rosa_laevigata RLG00000031253
rosa_multiflora Rmu_sc0002210.1_g000004
rosa_roxburghii Rroxscaffold_1G00070860
rosa_rugosa Rorug04G0413400
rosa_samantha Rh5AG041000 Rh5BG040800 Rh5CG044500 Rh5DG040700
rosa_wichuraiana Rw5G003950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2617
AatII GACGTC 1 cut(s) 2841
Acc36I ACCTGC 3 cut(s) 1403, 1618, 2617
AccB1I GGYRCC 1 cut(s) 2082
AccII CGCG 1 cut(s) 241
AciI CCGC 8 cut(s) 202, 241, 449, 596, 797, 2250, 3206, 3299
AclWI GGATC 7 cut(s) 837, 1856, 2020, 2739, 2863, 3094, 3314
AcoI YGGCCR 2 cut(s) 3225, 3300
AcsI RAATTY 7 cut(s) 289, 315, 570, 1371, 1888, 2034, 3568
AcuI CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
AcyI GRCGYC 3 cut(s) 63, 2083, 2838
AfaI GTAC 3 cut(s) 157, 2499, 3234
AfiI CCNNNNNNNGG 8 cut(s) 448, 770, 796, 1884, 2009, 2619, 3098, 3416
AflII CTTAAG 2 cut(s) 664, 3340
AhlI ACTAGT 4 cut(s) 401, 932, 3023, 3275
AjnI CCWGG 4 cut(s) 2085, 2557, 2665, 3385
AjuI GAANNNNNNNTTGG 2 cut(s) 1858, 1890
Alw21I GWGCWC 2 cut(s) 1675, 1984
Alw26I GTCTC 4 cut(s) 1672, 1804, 2085, 3153
AlwI GGATC 7 cut(s) 837, 1856, 2020, 2739, 2863, 3094, 3314
AlwNI CAGNNNCTG 2 cut(s) 1937, 2672
AoxI GGCC 7 cut(s) 712, 1665, 3100, 3225, 3300, 3522, 3543
ApoI RAATTY 7 cut(s) 289, 315, 570, 1371, 1888, 2034, 3568
ArsI GACNNNNNNTTYG 2 cut(s) 3175, 3207
AseI ATTAAT 2 cut(s) 1194, 2787
AspLEI GCGC 4 cut(s) 196, 241, 2085, 2594
AspS9I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
AsuC2I CCSGG 1 cut(s) 2003
AsuHPI GGTGA 7 cut(s) 88, 1088, 2255, 2408, 2598, 3081, 3493
AsuII TTCGAA 1 cut(s) 3538
AvaII GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3383
AxyI CCTNAGG 2 cut(s) 306, 525
BaeI ACNNNNGTAYC 2 cut(s) 2793, 2826
BalI TGGCCA 1 cut(s) 3227
BanI GGYRCC 1 cut(s) 2082
BanII GRGCYC 1 cut(s) 1297
BarI GAAGNNNNNNTAC 2 cut(s) 3297, 3329
BbsI GAAGAC 4 cut(s) 876, 2595, 3009, 3397
Bbv12I GWGCWC 2 cut(s) 1675, 1984
BbvCI CCTCAGC 2 cut(s) 392, 708
BccI CCATC 6 cut(s) 40, 1284, 1565, 2149, 2240, 2482
BceAI ACGGC 1 cut(s) 3287
BciT130I CCWGG 4 cut(s) 2087, 2559, 2667, 3387
BclI TGATCA 1 cut(s) 1207
BcnI CCSGG 1 cut(s) 2003
BcoDI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BcuI ACTAGT 4 cut(s) 401, 932, 3023, 3275
BfmI CTRYAG 1 cut(s) 2334
BfoI RGCGCY 2 cut(s) 2086, 2595
BfrI CTTAAG 2 cut(s) 664, 3340
BfuAI ACCTGC 3 cut(s) 1403, 1618, 2617
BglII AGATCT 1 cut(s) 2758
Bme1390I CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3387
Bme18I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3383
BmgT120I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
BmiI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3579
BmrFI CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3387
BpiI GAAGAC 4 cut(s) 876, 2595, 3009, 3397
BplI GAGNNNNNCTC 2 cut(s) 2569, 2601
BpmI CTGGAG 7 cut(s) 26, 750, 965, 1998, 2649, 3048, 3476
Bpu10I CCTNAGC 3 cut(s) 392, 708, 1883
Bpu14I TTCGAA 1 cut(s) 3538
BpuEI CTTGAG 7 cut(s) 653, 1281, 1754, 2571, 3017, 3257, 3363
BpuMI CCSGG 1 cut(s) 2003
BsaBI GATNNNNATC 1 cut(s) 828
BsaHI GRCGYC 3 cut(s) 63, 2083, 2838
BsaJI CCNNGG 8 cut(s) 1014, 2086, 2097, 2666, 3228, 3519, 3525, 3573
BsaWI WCCGGW 1 cut(s) 3041
BsaXI ACNNNNNCTCC 2 cut(s) 1824, 1854
Bsc4I CCNNNNNNNGG 8 cut(s) 448, 770, 796, 1884, 2009, 2619, 3098, 3416
Bse118I RCCGGY 1 cut(s) 22
Bse1I ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
Bse21I CCTNAGG 2 cut(s) 306, 525
Bse3DI GCAATG 4 cut(s) 118, 1437, 1504, 1593
Bse8I GATNNNNATC 1 cut(s) 828
BseBI CCWGG 4 cut(s) 2087, 2559, 2667, 3387
BseDI CCNNGG 8 cut(s) 1014, 2086, 2097, 2666, 3228, 3519, 3525, 3573
BseJI GATNNNNATC 1 cut(s) 828
BseLI CCNNNNNNNGG 8 cut(s) 448, 770, 796, 1884, 2009, 2619, 3098, 3416
BseMI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BseNI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BseRI GAGGAG 4 cut(s) 444, 753, 2337, 2926
BseX3I CGGCCG 1 cut(s) 3300
BseYI CCCAGC 1 cut(s) 2281
BsgI GTGCAG 4 cut(s) 1092, 1457, 2364, 3525
Bsh1236I CGCG 1 cut(s) 241
Bsh1285I CGRYCG 1 cut(s) 3303
BshFI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BshNI GGYRCC 1 cut(s) 2082
BsiEI CGRYCG 1 cut(s) 3303
BsiHKAI GWGCWC 2 cut(s) 1675, 1984
BsiSI CCGG 4 cut(s) 23, 124, 2003, 3042
BslFI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3324
BslI CCNNNNNNNGG 8 cut(s) 448, 770, 796, 1884, 2009, 2619, 3098, 3416
BsmAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BsmFI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3324
BsmI GAATGC 2 cut(s) 2329, 3400
BsnI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
Bsp119I TTCGAA 1 cut(s) 3538
Bsp1286I GDGCHC 3 cut(s) 1297, 1675, 1984
Bsp1407I TGTACA 1 cut(s) 155
BspACI CCGC 8 cut(s) 202, 241, 449, 596, 797, 2250, 3206, 3299
BspANI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BspFNI CGCG 1 cut(s) 241
BspHI TCATGA 1 cut(s) 3265
BspLI GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3579
BspMI ACCTGC 3 cut(s) 1403, 1618, 2617
BspPI GGATC 7 cut(s) 837, 1856, 2020, 2739, 2863, 3094, 3314
BspQI GCTCTTC 1 cut(s) 3540
BspT104I TTCGAA 1 cut(s) 3538
BspT107I GGYRCC 1 cut(s) 2082
BspTI CTTAAG 2 cut(s) 664, 3340
BsrDI GCAATG 4 cut(s) 118, 1437, 1504, 1593
BsrFI RCCGGY 1 cut(s) 22
BsrGI TGTACA 1 cut(s) 155
BsrI ACTGG 6 cut(s) 384, 767, 2657, 2724, 3117, 3287
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 8 cut(s) 1014, 2086, 2097, 2666, 3228, 3519, 3525, 3573
BssNI GRCGYC 3 cut(s) 63, 2083, 2838
BssT1I CCWWGG 3 cut(s) 1014, 3228, 3525
Bst2UI CCWGG 4 cut(s) 2087, 2559, 2667, 3387
Bst4CI ACNGT 5 cut(s) 74, 868, 1522, 3008, 3093
BstACI GRCGYC 3 cut(s) 63, 2083, 2838
BstAFI CTTAAG 2 cut(s) 664, 3340
BstAPI GCANNNNNTGC 1 cut(s) 1418
BstAUI TGTACA 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 3538
BstFNI CGCG 1 cut(s) 241
BstH2I RGCGCY 2 cut(s) 2086, 2595
BstHHI GCGC 4 cut(s) 196, 241, 2085, 2594
BstMAI GTCTC 4 cut(s) 1672, 1804, 2085, 3153
BstMCI CGRYCG 1 cut(s) 3303
BstNI CCWGG 4 cut(s) 2087, 2559, 2667, 3387
BstNSI RCATGY 2 cut(s) 1793, 1960
BstSCI CCNGG 5 cut(s) 2001, 2085, 2557, 2665, 3385
BstSFI CTRYAG 1 cut(s) 2334
BstUI CGCG 1 cut(s) 241
BstV2I GAAGAC 4 cut(s) 876, 2595, 3009, 3397
BstX2I RGATCY 3 cut(s) 829, 2744, 2758
BstXI CCANNNNNNTGG 1 cut(s) 332
BstYI RGATCY 3 cut(s) 829, 2744, 2758
BstZI CGGCCG 1 cut(s) 3300
Bsu36I CCTNAGG 2 cut(s) 306, 525
BsuRI GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
BtgZI GCGATG 1 cut(s) 204
BtsIMutI CAGTG 7 cut(s) 35, 70, 774, 1109, 2233, 2301, 3294
BveI ACCTGC 3 cut(s) 1403, 1618, 2617
CaiI CAGNNNCTG 2 cut(s) 1937, 2672
CciI TCATGA 1 cut(s) 3265
CfoI GCGC 4 cut(s) 196, 241, 2085, 2594
Cfr10I RCCGGY 1 cut(s) 22
Cfr13I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
CseI GACGC 3 cut(s) 71, 524, 2981
Csp6I GTAC 3 cut(s) 156, 2498, 3233
CviAII CATG 9 cut(s) 414, 443, 1784, 1790, 1879, 1957, 2438, 3083, 3266
CviQI GTAC 3 cut(s) 156, 2498, 3233
DinI GGCGCC 1 cut(s) 2084
EaeI YGGCCR 2 cut(s) 3225, 3300
EagI CGGCCG 1 cut(s) 3300
EclXI CGGCCG 1 cut(s) 3300
Eco130I CCWWGG 3 cut(s) 1014, 3228, 3525
Eco147I AGGCCT 1 cut(s) 3524
Eco24I GRGCYC 1 cut(s) 1297
Eco47I GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3383
Eco52I CGGCCG 1 cut(s) 3300
Eco57I CTGAAG 7 cut(s) 1007, 1104, 1326, 1725, 2484, 2493, 3194
Eco81I CCTNAGG 2 cut(s) 306, 525
EcoO109I RGGNCCY 4 cut(s) 1255, 1468, 2278, 3383
EcoRI GAATTC 1 cut(s) 3568
EcoRII CCWGG 4 cut(s) 2085, 2557, 2665, 3385
EcoT14I CCWWGG 3 cut(s) 1014, 3228, 3525
EcoT22I ATGCAT 1 cut(s) 2443
EcoT38I GRGCYC 1 cut(s) 1297
EgeI GGCGCC 1 cut(s) 2084
EheI GGCGCC 1 cut(s) 2084
ErhI CCWWGG 3 cut(s) 1014, 3228, 3525
FaeI CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
FalI AAGNNNNNCTT 2 cut(s) 774, 806
FaqI GGGAC 5 cut(s) 1471, 1481, 1701, 2207, 3324
FatI CATG 9 cut(s) 413, 442, 1783, 1789, 1878, 1956, 2437, 3082, 3265
FauI CCCGC 1 cut(s) 2243
FbaI TGATCA 1 cut(s) 1207
FriOI GRGCYC 1 cut(s) 1297
GlaI GCGC 4 cut(s) 195, 240, 2084, 2593
GsaI CCCAGC 1 cut(s) 2285
GsuI CTGGAG 7 cut(s) 26, 750, 965, 1998, 2649, 3048, 3476
HaeII RGCGCY 2 cut(s) 2086, 2595
HaeIII GGCC 7 cut(s) 714, 1667, 3102, 3227, 3302, 3524, 3545
HapII CCGG 4 cut(s) 23, 124, 2003, 3042
HgaI GACGC 3 cut(s) 71, 524, 2981
HhaI GCGC 4 cut(s) 196, 241, 2085, 2594
Hin1I GRCGYC 3 cut(s) 63, 2083, 2838
Hin1II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
Hin6I GCGC 4 cut(s) 194, 239, 2083, 2592
HinP1I GCGC 4 cut(s) 194, 239, 2083, 2592
HincII GTYRAC 4 cut(s) 61, 367, 1348, 2719
HindII GTYRAC 4 cut(s) 61, 367, 1348, 2719
HindIII AAGCTT 2 cut(s) 543, 1001
HpaI GTTAAC 2 cut(s) 367, 2719
HpaII CCGG 4 cut(s) 23, 124, 2003, 3042
HphI GGTGA 7 cut(s) 88, 1088, 2255, 2408, 2598, 3081, 3493
Hpy166II GTNNAC 7 cut(s) 61, 96, 158, 367, 1348, 2540, 2719
Hpy8I GTNNAC 7 cut(s) 61, 96, 158, 367, 1348, 2540, 2719
Hpy99I CGWCG 1 cut(s) 3097
HpyCH4III ACNGT 5 cut(s) 74, 868, 1522, 3008, 3093
HpyCH4IV ACGT 3 cut(s) 2838, 3163, 3502
HpySE526I ACGT 3 cut(s) 2838, 3163, 3502
Hsp92I GRCGYC 3 cut(s) 63, 2083, 2838
Hsp92II CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
HspAI GCGC 4 cut(s) 194, 239, 2083, 2592
KasI GGCGCC 1 cut(s) 2082
KroI GCCGGC 1 cut(s) 22
KroNI GCCGGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 1207
KspAI GTTAAC 2 cut(s) 367, 2719
LguI GCTCTTC 1 cut(s) 3540
LmnI GCTCC 5 cut(s) 628, 799, 1979, 2410, 3583
MaeII ACGT 3 cut(s) 2838, 3163, 3502
MfeI CAATTG 1 cut(s) 3348
MflI RGATCY 3 cut(s) 829, 2744, 2758
MhlI GDGCHC 3 cut(s) 1297, 1675, 1984
MlsI TGGCCA 1 cut(s) 3227
MluNI TGGCCA 1 cut(s) 3227
Mly113I GGCGCC 1 cut(s) 2083
MlyI GAGTC 6 cut(s) 722, 868, 1097, 1493, 1763, 3155
MmeI TCCRAC 9 cut(s) 633, 1877, 1889, 1987, 2130, 2160, 2502, 2964, 3173
Mox20I TGGCCA 1 cut(s) 3227
Mph1103I ATGCAT 1 cut(s) 2443
MroNI GCCGGC 1 cut(s) 22
MscI TGGCCA 1 cut(s) 3227
MslI CAYNNNNRTG 2 cut(s) 1788, 1794
Msp20I TGGCCA 1 cut(s) 3227
MspA1I CMGCKG 4 cut(s) 153, 797, 985, 3068
MspCI CTTAAG 2 cut(s) 664, 3340
MspI CCGG 4 cut(s) 23, 124, 2003, 3042
MspR9I CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3387
MunI CAATTG 1 cut(s) 3348
Mva1269I GAATGC 2 cut(s) 2329, 3400
MvaI CCWGG 4 cut(s) 2087, 2559, 2667, 3387
MvnI CGCG 1 cut(s) 241
NaeI GCCGGC 1 cut(s) 24
NarI GGCGCC 1 cut(s) 2083
NciI CCSGG 1 cut(s) 2003
NgoMIV GCCGGC 1 cut(s) 22
NlaIII CATG 9 cut(s) 417, 446, 1787, 1793, 1882, 1960, 2441, 3086, 3269
NlaIV GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3579
NmeAIII GCCGAG 2 cut(s) 3544, 3554
NmuCI GTSAC 5 cut(s) 757, 836, 1234, 3155, 3425
NsiI ATGCAT 1 cut(s) 2443
NspI RCATGY 2 cut(s) 1793, 1960
NspV TTCGAA 1 cut(s) 3538
PaeI GCATGC 1 cut(s) 1793
PagI TCATGA 1 cut(s) 3265
PaqCI CACCTGC 1 cut(s) 2617
PceI AGGCCT 1 cut(s) 3524
PciSI GCTCTTC 1 cut(s) 3540
PctI GAATGC 2 cut(s) 2329, 3400
PdiI GCCGGC 1 cut(s) 24
PfeI GAWTC 9 cut(s) 350, 434, 1114, 1301, 1633, 1729, 1970, 2711, 3269
PfoI TCCNGGA 1 cut(s) 3385
PleI GAGTC 6 cut(s) 722, 867, 1096, 1492, 1763, 3154
PluTI GGCGCC 1 cut(s) 2086
PpsI GAGTC 6 cut(s) 722, 867, 1096, 1492, 1763, 3154
PpuMI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
PshBI ATTAAT 2 cut(s) 1194, 2787
Psp5II RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
Psp6I CCWGG 4 cut(s) 2085, 2557, 2665, 3385
PspFI CCCAGC 1 cut(s) 2281
PspGI CCWGG 4 cut(s) 2085, 2557, 2665, 3385
PspN4I GGNNCC 6 cut(s) 1257, 1469, 2084, 2280, 2556, 3579
PspPI GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
PspPPI RGGWCCY 4 cut(s) 1255, 1468, 2278, 3383
PsrI GAACNNNNNNTAC 2 cut(s) 2490, 2522
PstNI CAGNNNCTG 2 cut(s) 1937, 2672
PsuI RGATCY 3 cut(s) 829, 2744, 2758
PvuII CAGCTG 3 cut(s) 153, 985, 3068
RsaI GTAC 3 cut(s) 157, 2499, 3234
RsaNI GTAC 3 cut(s) 156, 2498, 3233
RseI CAYNNNNRTG 2 cut(s) 1788, 1794
SapI GCTCTTC 1 cut(s) 3540
Sau96I GGNCC 9 cut(s) 712, 1255, 1468, 1666, 2278, 2555, 3100, 3383, 3543
SchI GAGTC 6 cut(s) 722, 868, 1097, 1493, 1763, 3155
ScrFI CCNGG 5 cut(s) 2003, 2087, 2559, 2667, 3387
SduI GDGCHC 3 cut(s) 1297, 1675, 1984
SfcI CTRYAG 1 cut(s) 2334
SfoI GGCGCC 1 cut(s) 2084
SfuI TTCGAA 1 cut(s) 3538
SinI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3383
SmiMI CAYNNNNRTG 2 cut(s) 1788, 1794
SmlI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3378
SmoI CTYRAG 9 cut(s) 632, 664, 1296, 1733, 2586, 2996, 3236, 3340, 3378
SpeI ACTAGT 4 cut(s) 401, 932, 3023, 3275
SphI GCATGC 1 cut(s) 1793
SseBI AGGCCT 1 cut(s) 3524
SsiI CCGC 8 cut(s) 202, 241, 449, 596, 797, 2250, 3206, 3299
SspDI GGCGCC 1 cut(s) 2082
SspI AATATT 1 cut(s) 1579
StuI AGGCCT 1 cut(s) 3524
StyD4I CCNGG 5 cut(s) 2001, 2085, 2557, 2665, 3385
StyI CCWWGG 3 cut(s) 1014, 3228, 3525
TaaI ACNGT 5 cut(s) 74, 868, 1522, 3008, 3093
TaiI ACGT 3 cut(s) 2841, 3166, 3505
TaqI TCGA 4 cut(s) 246, 427, 3029, 3538
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 3302
TfiI GAWTC 9 cut(s) 350, 434, 1114, 1301, 1633, 1729, 1970, 2711, 3269
TscAI CASTG 7 cut(s) 42, 77, 774, 1116, 2233, 2301, 3294
TseFI GTSAC 5 cut(s) 757, 836, 1234, 3155, 3425
Tsp45I GTSAC 5 cut(s) 757, 836, 1234, 3155, 3425
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 7 cut(s) 42, 77, 774, 1116, 2233, 2301, 3294
Vha464I CTTAAG 2 cut(s) 664, 3340
VpaK11BI GGWCC 5 cut(s) 1255, 1468, 2278, 2555, 3383
VspI ATTAAT 2 cut(s) 1194, 2787
XapI RAATTY 7 cut(s) 289, 315, 570, 1371, 1888, 2034, 3568
XbaI TCTAGA 1 cut(s) 1042
XceI RCATGY 2 cut(s) 1793, 1960
XcmI CCANNNNNNNNNTGG 2 cut(s) 1429, 3080
ZraI GACGTC 1 cut(s) 2839
Zsp2I ATGCAT 1 cut(s) 2443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.