FvH4_3g04090

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008029
Physical Location & Seq
Forward (+)
2279559 .. 2283961
4403 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04090.t1

Sequence Viewer

Length: 1686 bp
ATGAAACTGAAAAACAAATATGCAACAAAAATGGTTTTTCGTGACTTTTGGTCAAATGCAACCAATATTGCAGTTGGCGTACGTTACCAACTTCCTCGTTCCCATCCAAAAACCCGGTCCAATTCTCTTTTTATAACCAAAAACCAACCCGCTTCTTCTTCCTCACCAAAACTTCGACTCGTCACCTCCGATATGGACCGGCCCGACTCCTCTCGGCCCGTCCTATCCGACCGTGTCCTTATAAACGGCGTCGTCACGCCGCTCACGTTCTTTGCCGACGGCGTTCTCCGGTGGACGGAGCGACGCCAGCGGACCTTGATCGTCGAGAAGGAGGTCCTCGGCCTCAAAGTGGAGGGTCCGAAGATCAGAATCGGAGCTCTGGTCCACGCCGGAGCTGGAATCTGCTGCGTCGCGAGCGGAGGAGGAGGAGGAGGAGCTCTGGTCAGGCGGGACTTGGTGTTCGAGCCTCTCTCCGAGGACTTGCGGAAGCTCTGGTGCCAGAAGCTTCTGGATTTCATCGATTCGCTTGGAAGGCCGAGGAGGCTGTTAGTTTTTGTGAATCCATTCGGAGGGAGCAAAGCTGCTTCGAAGATATTCGCAGATGAAGTGAAGCCGCTTTTTGATGATGCTGGTGTTCAGCTTACACTGCAAGAAACTCAGTATCAACTGCATGCCAAGGAAGTTGTGAAAACTCTGGATCTTTCCAAGTATGATGGCATTGTTTGCGTTAGTGGGGATGGAGTCTTGGTTGAGGTAGTAAATGGACTCCTTGAGAGAGAAGATTGGGACACTGCAATTAAGATGCCTCTTGGAGTAGTTCCTGCAGGTACTGGGAATGGAATGGCGAAATCTCTTCTAGAGTCTGTTGGTGAACCTTGTAAAGTGACGAATGCTGTTCTCGCCATTATTAGAGGACATAAGCGATCATTGGATGTATCTACCATATGGCAGGGGAAGACAAAATTTTTTAGCGTGTTGATGCTTGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTTTAGATATCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCACCTGGCTTCGAAGGTTTTGGGGAGCCTACTAGTTACAAGGACGGTGGTGAATCGACAAACACTGATCCAAGTCAAGAGAAGCCTGTTAAGGTTCGACAACTTGGCTATGAGGGTCCTGATATTAGCTTGGAGAACATGAACTGGAGAACAATTGAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGAAACAAAGGCAGCTCCTGATGCCGAGTTCTCAGATGGCTATCTAGATGTTATAATTATGAAGGCTTGTCCAAAGTTATCCTTGCTCTCGCTAATGACAGGGTTGAGCAGGGGGACTCATGTCAAGTCACCATATGTGTCGTACTTCAAGGTGAAAGCATTTGTTTTGGAGCCTGGTGCTCGTGCTGAGGATCCAACGAAGGAAGGGATAGTAGACTCGGATGGTGAGGTACTCGCTAGAGGCAAAGGAGCATACAAATGTCAGCAGAAGACACTAATGTCATACGATAAACTTCAAATAACTGTGGATCAAGGTTTGGCCACTCTGTTTACCCCAGAACAGAATATACACTTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

562

Amino Acids

61.79

Weight (kDa)

8.72

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 181 - 314 3.7e-31 Diacylglycerol kinase catalytic domain
YegS_C PF19279 430 - 523 3.3e-12 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 134, 242, 1379
AasI GACNNNNNNGTC 1 cut(s) 1603
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 495
AccBSI CCGCTC 2 cut(s) 262, 417
AccI GTMKAC 1 cut(s) 1539
AccII CGCG 1 cut(s) 413
AciI CCGC 7 cut(s) 150, 260, 310, 417, 448, 484, 614
AclWI GGATC 6 cut(s) 705, 1108, 1181, 1511, 1524, 1641
AcoI YGGCCR 1 cut(s) 1644
AcsI RAATTY 1 cut(s) 962
AcyI GRCGYC 2 cut(s) 249, 304
AfaI GTAC 5 cut(s) 81, 829, 1311, 1469, 1557
AfiI CCNNNNNNNGG 5 cut(s) 295, 349, 569, 1124, 1319
AgsI TTSAA 4 cut(s) 1070, 1277, 1474, 1622
AhdI GACNNNNNGTC 2 cut(s) 49, 1015
AhlI ACTAGT 1 cut(s) 1151
AjnI CCWGG 2 cut(s) 1123, 1498
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
AluBI AGCT 9 cut(s) 377, 395, 437, 490, 505, 581, 640, 1248, 1340
AluI AGCT 9 cut(s) 377, 395, 437, 490, 505, 581, 640, 1248, 1340
Alw21I GWGCWC 4 cut(s) 379, 439, 1048, 1507
AlwI GGATC 6 cut(s) 705, 1108, 1181, 1511, 1524, 1641
AlwNI CAGNNNCTG 2 cut(s) 830, 1343
AoxI GGCC 5 cut(s) 200, 215, 340, 533, 1644
ApeKI GCWGC 3 cut(s) 405, 581, 1337
ApoI RAATTY 1 cut(s) 962
ArsI GACNNNNNNTTYG 4 cut(s) 101, 133, 443, 475
Asp700I GAANNNNTTC 2 cut(s) 563, 593
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 8 cut(s) 156, 175, 881, 1181, 1334, 1446, 1489, 1562
AsuII TTCGAA 2 cut(s) 587, 1131
AvaII GGWCC 8 cut(s) 117, 196, 312, 334, 356, 382, 1235, 1280
BalI TGGCCA 1 cut(s) 1646
BamHI GGATCC 1 cut(s) 1516
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 2 cut(s) 379, 439
BauI CACGAG 1 cut(s) 1506
BbsI GAAGAC 2 cut(s) 962, 1601
Bbv12I GWGCWC 4 cut(s) 379, 439, 1048, 1507
BbvCI CCTCAGC 1 cut(s) 1512
BbvI GCAGC 3 cut(s) 392, 568, 1349
BccI CCATC 6 cut(s) 111, 707, 731, 1030, 1355, 1541
BceAI ACGGC 2 cut(s) 262, 295
BciT130I CCWGG 2 cut(s) 1125, 1500
BcnI CCSGG 1 cut(s) 115
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 4 cut(s) 857, 1152, 1370, 1563
BfmI CTRYAG 1 cut(s) 822
BfuAI ACCTGC 1 cut(s) 815
BglI GCCNNNNNGGC 1 cut(s) 541
BisI GCNGC 5 cut(s) 260, 406, 582, 614, 1338
BlsI GCNGC 5 cut(s) 261, 407, 583, 615, 1339
Bme1390I CCNGG 3 cut(s) 115, 1125, 1500
Bme18I GGWCC 8 cut(s) 117, 196, 312, 334, 356, 382, 1235, 1280
BmeRI GACNNNNNGTC 2 cut(s) 49, 1015
BmiI GGNNCC 6 cut(s) 357, 497, 1146, 1236, 1497, 1518
BmrFI CCNGG 3 cut(s) 115, 1125, 1500
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 4 cut(s) 616, 792, 969, 1336
BmuI ACTGGG 1 cut(s) 840
BoxI GACNNNNGTC 1 cut(s) 1445
BpiI GAAGAC 2 cut(s) 962, 1601
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpmI CTGGAG 1 cut(s) 1285
Bpu10I CCTNAGC 1 cut(s) 1512
Bpu14I TTCGAA 2 cut(s) 587, 1131
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 1 cut(s) 115
Bsa29I ATCGAT 1 cut(s) 519
BsaBI GATNNNNATC 2 cut(s) 368, 1365
BsaHI GRCGYC 2 cut(s) 249, 304
BsaJI CCNNGG 5 cut(s) 337, 474, 536, 675, 1313
BsaWI WCCGGW 1 cut(s) 288
BsaXI ACNNNNNCTCC 6 cut(s) 366, 396, 426, 456, 732, 762
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 349, 569, 1124, 1319
Bse118I RCCGGY 1 cut(s) 198
Bse1I ACTGG 2 cut(s) 835, 1268
Bse8I GATNNNNATC 2 cut(s) 368, 1365
BseBI CCWGG 2 cut(s) 1125, 1500
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 5 cut(s) 337, 474, 536, 675, 1313
BseGI GGATG 5 cut(s) 103, 742, 937, 1041, 1552
BseJI GATNNNNATC 2 cut(s) 368, 1365
BseLI CCNNNNNNNGG 5 cut(s) 295, 349, 569, 1124, 1319
BseMII CTCAG 3 cut(s) 671, 1371, 1503
BseNI ACTGG 2 cut(s) 835, 1268
BseRI GAGGAG 7 cut(s) 199, 435, 438, 441, 444, 447, 553
BseXI GCAGC 3 cut(s) 392, 568, 1349
BsgI GTGCAG 1 cut(s) 1104
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 5 cut(s) 202, 217, 342, 535, 1646
BshNI GGYRCC 1 cut(s) 495
BshVI ATCGAT 1 cut(s) 519
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 4 cut(s) 379, 439, 1048, 1507
BsiSI CCGG 4 cut(s) 115, 199, 289, 390
BsiWI CGTACG 1 cut(s) 79
BslFI GGGAC 3 cut(s) 464, 800, 1453
BslI CCNNNNNNNGG 5 cut(s) 295, 349, 569, 1124, 1319
BsmFI GGGAC 3 cut(s) 464, 800, 1453
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 5 cut(s) 202, 217, 342, 535, 1646
Bsp119I TTCGAA 2 cut(s) 587, 1131
Bsp1286I GDGCHC 4 cut(s) 379, 439, 1048, 1507
Bsp143I GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
Bsp68I TCGCGA 1 cut(s) 413
BspACI CCGC 7 cut(s) 150, 260, 310, 417, 448, 484, 614
BspANI GGCC 5 cut(s) 202, 217, 342, 535, 1646
BspCNI CTCAG 3 cut(s) 670, 1370, 1504
BspDI ATCGAT 1 cut(s) 519
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 1682
BspLI GGNNCC 6 cut(s) 357, 497, 1146, 1236, 1497, 1518
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 6 cut(s) 705, 1108, 1181, 1511, 1524, 1641
BspT104I TTCGAA 2 cut(s) 587, 1131
BspT107I GGYRCC 1 cut(s) 495
BsrBI CCGCTC 2 cut(s) 262, 417
BsrFI RCCGGY 1 cut(s) 198
BsrI ACTGG 2 cut(s) 835, 1268
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 5 cut(s) 337, 474, 536, 675, 1313
BssMI GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
BssNI GRCGYC 2 cut(s) 249, 304
BssSI CACGAG 1 cut(s) 1506
BssT1I CCWWGG 2 cut(s) 675, 1313
Bst2BI CACGAG 1 cut(s) 1506
Bst2UI CCWGG 2 cut(s) 1125, 1500
Bst4CI ACNGT 4 cut(s) 233, 1166, 1284, 1630
Bst6I CTCTTC 1 cut(s) 858
BstACI GRCGYC 2 cut(s) 249, 304
BstAPI GCANNNNNTGC 1 cut(s) 723
BstBI TTCGAA 2 cut(s) 587, 1131
BstC8I GCNNGC 5 cut(s) 308, 415, 672, 984, 1119
BstDEI CTNAG 4 cut(s) 657, 994, 1357, 1512
BstENI CCTNNNNNAGG 1 cut(s) 1317
BstF5I GGATG 5 cut(s) 103, 742, 937, 1041, 1552
BstFNI CGCG 1 cut(s) 413
BstKTI GATC 8 cut(s) 321, 366, 700, 926, 1103, 1189, 1519, 1636
BstMBI GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 9 cut(s) 307, 414, 532, 541, 646, 723, 899, 1087, 1346
BstNI CCWGG 2 cut(s) 1125, 1500
BstNSI RCATGY 1 cut(s) 674
BstPAI GACNNNNGTC 1 cut(s) 1445
BstSCI CCNGG 3 cut(s) 113, 1123, 1498
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 3 cut(s) 392, 568, 1349
BstV2I GAAGAC 2 cut(s) 962, 1601
BstX2I RGATCY 3 cut(s) 697, 1100, 1516
BstYI RGATCY 3 cut(s) 697, 1100, 1516
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 5 cut(s) 202, 217, 342, 535, 1646
BsuTUI ATCGAT 1 cut(s) 519
BtsCI GGATG 5 cut(s) 103, 742, 937, 1041, 1552
BtsI GCAGTG 2 cut(s) 644, 789
BtsIMutI CAGTG 4 cut(s) 644, 789, 1182, 1299
BtuMI TCGCGA 1 cut(s) 413
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 5 cut(s) 308, 415, 672, 984, 1119
CaiI CAGNNNCTG 2 cut(s) 830, 1343
CciI TCATGA 1 cut(s) 1682
Cfr10I RCCGGY 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 519
CseI GACGC 3 cut(s) 238, 312, 397
Csp6I GTAC 5 cut(s) 80, 828, 1310, 1468, 1556
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviAII CATG 5 cut(s) 671, 987, 1258, 1445, 1683
CviQI GTAC 5 cut(s) 80, 828, 1310, 1468, 1556
DdeI CTNAG 4 cut(s) 657, 994, 1357, 1512
DpnI GATC 8 cut(s) 320, 365, 699, 925, 1102, 1188, 1518, 1635
DpnII GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
DrdI GACNNNNNNGTC 1 cut(s) 1603
DriI GACNNNNNGTC 2 cut(s) 49, 1015
DseDI GACNNNNNNGTC 1 cut(s) 1603
EaeI YGGCCR 1 cut(s) 1644
Eam1104I CTCTTC 1 cut(s) 858
Eam1105I GACNNNNNGTC 2 cut(s) 49, 1015
EarI CTCTTC 1 cut(s) 858
Ecl136II GAGCTC 2 cut(s) 377, 437
Eco130I CCWWGG 2 cut(s) 675, 1313
Eco24I GRGCYC 2 cut(s) 379, 439
Eco32I GATATC 1 cut(s) 1057
Eco47I GGWCC 8 cut(s) 117, 196, 312, 334, 356, 382, 1235, 1280
Eco53kI GAGCTC 2 cut(s) 377, 437
EcoICRI GAGCTC 2 cut(s) 377, 437
EcoNI CCTNNNNNAGG 1 cut(s) 1317
EcoO109I RGGNCCY 2 cut(s) 334, 1235
EcoRII CCWGG 2 cut(s) 1123, 1498
EcoRV GATATC 1 cut(s) 1057
EcoT14I CCWWGG 2 cut(s) 675, 1313
EcoT38I GRGCYC 2 cut(s) 379, 439
ErhI CCWWGG 2 cut(s) 675, 1313
FaeI CATG 5 cut(s) 674, 990, 1261, 1448, 1686
FalI AAGNNNNNCTT 2 cut(s) 1391, 1423
FaqI GGGAC 3 cut(s) 464, 800, 1453
FatI CATG 5 cut(s) 670, 986, 1257, 1444, 1682
FauI CCCGC 2 cut(s) 157, 441
FauNDI CATATG 2 cut(s) 944, 1459
FblI GTMKAC 1 cut(s) 1539
Fnu4HI GCNGC 5 cut(s) 260, 406, 582, 614, 1338
FokI GGATG 5 cut(s) 90, 749, 944, 1048, 1559
FriOI GRGCYC 2 cut(s) 379, 439
Fsp4HI GCNGC 5 cut(s) 260, 406, 582, 614, 1338
FspBI CTAG 4 cut(s) 857, 1152, 1370, 1563
GluI GCNGC 5 cut(s) 260, 406, 582, 614, 1338
GsuI CTGGAG 1 cut(s) 1285
HaeIII GGCC 5 cut(s) 202, 217, 342, 535, 1646
HapII CCGG 4 cut(s) 115, 199, 289, 390
HgaI GACGC 3 cut(s) 238, 312, 397
Hin1I GRCGYC 2 cut(s) 249, 304
Hin1II CATG 5 cut(s) 674, 990, 1261, 1448, 1686
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HindIII AAGCTT 1 cut(s) 503
HpaII CCGG 4 cut(s) 115, 199, 289, 390
HphI GGTGA 8 cut(s) 156, 175, 881, 1181, 1334, 1446, 1489, 1562
Hpy166II GTNNAC 6 cut(s) 294, 385, 872, 1009, 1540, 1656
Hpy8I GTNNAC 6 cut(s) 294, 385, 872, 1009, 1540, 1656
Hpy99I CGWCG 5 cut(s) 254, 281, 306, 326, 413
HpyAV CCTTC 7 cut(s) 322, 525, 1127, 1271, 1381, 1519, 1523
HpyCH4III ACNGT 4 cut(s) 233, 1166, 1284, 1630
HpyCH4IV ACGT 2 cut(s) 82, 266
HpyF10VI GCNNNNNNNGC 9 cut(s) 307, 414, 532, 541, 646, 723, 899, 1087, 1346
HpyF3I CTNAG 4 cut(s) 657, 994, 1357, 1512
HpySE526I ACGT 2 cut(s) 82, 266
Hsp92I GRCGYC 2 cut(s) 249, 304
Hsp92II CATG 5 cut(s) 674, 990, 1261, 1448, 1686
Kzo9I GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
LmnI GCTCC 9 cut(s) 298, 374, 392, 434, 573, 1144, 1345, 1495, 1574
Lsp1109I GCAGC 3 cut(s) 392, 568, 1349
LweI GCATC 4 cut(s) 616, 792, 969, 1336
MaeI CTAG 4 cut(s) 857, 1152, 1370, 1563
MaeII ACGT 2 cut(s) 82, 266
MaeIII GTNAC 7 cut(s) 41, 83, 181, 253, 883, 1154, 1452
MalI GATC 8 cut(s) 320, 365, 699, 925, 1102, 1188, 1518, 1635
MbiI CCGCTC 2 cut(s) 262, 417
MboI GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
MboII GAAGA 9 cut(s) 147, 150, 373, 601, 791, 845, 967, 1337, 1606
MfeI CAATTG 1 cut(s) 1272
MflI RGATCY 3 cut(s) 697, 1100, 1516
MhlI GDGCHC 4 cut(s) 379, 439, 1048, 1507
MlsI TGGCCA 1 cut(s) 1646
MluCI AATT 6 cut(s) 121, 795, 962, 1074, 1272, 1380
MluNI TGGCCA 1 cut(s) 1646
MlyI GAGTC 8 cut(s) 171, 200, 750, 759, 869, 1025, 1435, 1535
MmeI TCCRAC 2 cut(s) 252, 1544
Mox20I TGGCCA 1 cut(s) 1646
MroXI GAANNNNTTC 2 cut(s) 563, 593
MscI TGGCCA 1 cut(s) 1646
MseI TTAA 2 cut(s) 798, 1209
MslI CAYNNNNRTG 1 cut(s) 1582
Msp20I TGGCCA 1 cut(s) 1646
MspA1I CMGCKG 1 cut(s) 310
MspI CCGG 4 cut(s) 115, 199, 289, 390
MspR9I CCNGG 3 cut(s) 115, 1125, 1500
MunI CAATTG 1 cut(s) 1272
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 2 cut(s) 1125, 1500
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 9 cut(s) 307, 414, 532, 541, 646, 723, 899, 1087, 1346
NciI CCSGG 1 cut(s) 115
NdeI CATATG 2 cut(s) 944, 1459
NdeII GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
NlaIII CATG 5 cut(s) 674, 990, 1261, 1448, 1686
NlaIV GGNNCC 6 cut(s) 357, 497, 1146, 1236, 1497, 1518
NmeAIII GCCGAG 4 cut(s) 193, 318, 561, 1375
NmuCI GTSAC 5 cut(s) 41, 181, 253, 883, 1452
NruI TCGCGA 1 cut(s) 413
NspI RCATGY 1 cut(s) 674
NspV TTCGAA 2 cut(s) 587, 1131
PaeI GCATGC 1 cut(s) 674
PagI TCATGA 1 cut(s) 1682
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 263
PctI GAATGC 1 cut(s) 895
PdmI GAANNNNTTC 2 cut(s) 563, 593
PfeI GAWTC 5 cut(s) 369, 399, 521, 559, 1172
Pfl23II CGTACG 1 cut(s) 79
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 5 cut(s) 261, 407, 583, 615, 1339
PleI GAGTC 8 cut(s) 171, 200, 749, 759, 868, 1024, 1435, 1535
PpsI GAGTC 8 cut(s) 171, 200, 749, 759, 868, 1024, 1435, 1535
PpuMI RGGWCCY 2 cut(s) 334, 1235
PshAI GACNNNNGTC 1 cut(s) 1445
PsiI TTATAA 3 cut(s) 134, 242, 1379
Psp124BI GAGCTC 2 cut(s) 379, 439
Psp5II RGGWCCY 2 cut(s) 334, 1235
Psp6I CCWGG 2 cut(s) 1123, 1498
PspGI CCWGG 2 cut(s) 1123, 1498
PspLI CGTACG 1 cut(s) 79
PspN4I GGNNCC 6 cut(s) 357, 497, 1146, 1236, 1497, 1518
PspPPI RGGWCCY 2 cut(s) 334, 1235
PsrI GAACNNNNNNTAC 1 cut(s) 1656
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 2 cut(s) 830, 1343
PsuI RGATCY 3 cut(s) 697, 1100, 1516
PsyI GACNNNGTC 1 cut(s) 233
RruI TCGCGA 1 cut(s) 413
RsaI GTAC 5 cut(s) 81, 829, 1311, 1469, 1557
RsaNI GTAC 5 cut(s) 80, 828, 1310, 1468, 1556
RseI CAYNNNNRTG 1 cut(s) 1582
SacI GAGCTC 2 cut(s) 379, 439
SaqAI TTAA 2 cut(s) 798, 1209
SatI GCNGC 5 cut(s) 260, 406, 582, 614, 1338
Sau3AI GATC 8 cut(s) 318, 363, 697, 923, 1100, 1186, 1516, 1633
SbfI CCTGCAGG 1 cut(s) 826
SchI GAGTC 8 cut(s) 171, 200, 750, 759, 869, 1025, 1435, 1535
ScrFI CCNGG 3 cut(s) 115, 1125, 1500
SdaI CCTGCAGG 1 cut(s) 826
SduI GDGCHC 4 cut(s) 379, 439, 1048, 1507
SfaNI GCATC 4 cut(s) 616, 792, 969, 1336
SfcI CTRYAG 1 cut(s) 822
SfuI TTCGAA 2 cut(s) 587, 1131
SinI GGWCC 8 cut(s) 117, 196, 312, 334, 356, 382, 1235, 1280
SmiMI CAYNNNNRTG 1 cut(s) 1582
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SpeI ACTAGT 1 cut(s) 1151
SphI GCATGC 1 cut(s) 674
Sse8387I CCTGCAGG 1 cut(s) 826
Sse9I AATT 6 cut(s) 121, 795, 962, 1074, 1272, 1380
SsiI CCGC 7 cut(s) 150, 260, 310, 417, 448, 484, 614
SspI AATATT 1 cut(s) 67
SspMI CTAG 4 cut(s) 857, 1152, 1370, 1563
SstI GAGCTC 2 cut(s) 379, 439
StyD4I CCNGG 3 cut(s) 113, 1123, 1498
StyI CCWWGG 2 cut(s) 675, 1313
TaaI ACNGT 4 cut(s) 233, 1166, 1284, 1630
TaiI ACGT 2 cut(s) 85, 269
TaqI TCGA 8 cut(s) 175, 324, 462, 519, 587, 1131, 1175, 1216
TasI AATT 6 cut(s) 121, 795, 962, 1074, 1272, 1380
TauI GCSGC 2 cut(s) 262, 616
TfiI GAWTC 5 cut(s) 369, 399, 521, 559, 1172
Tru1I TTAA 2 cut(s) 798, 1209
Tru9I TTAA 2 cut(s) 798, 1209
TscAI CASTG 4 cut(s) 651, 796, 1189, 1299
TseFI GTSAC 5 cut(s) 41, 181, 253, 883, 1452
TseI GCWGC 3 cut(s) 405, 581, 1337
Tsp45I GTSAC 5 cut(s) 41, 181, 253, 883, 1452
TspDTI ATGAA 6 cut(s) 17, 505, 618, 1274, 1400, 1671
TspGWI ACGGA 2 cut(s) 311, 1113
TspRI CASTG 4 cut(s) 651, 796, 1189, 1299
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 8 cut(s) 117, 196, 312, 334, 356, 382, 1235, 1280
XagI CCTNNNNNAGG 1 cut(s) 1317
XapI RAATTY 1 cut(s) 962
XbaI TCTAGA 2 cut(s) 856, 1369
XceI RCATGY 1 cut(s) 674
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 1539
XmnI GAANNNNTTC 2 cut(s) 563, 593
XspI CTAG 4 cut(s) 857, 1152, 1370, 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.