RchiOBHm_Chr5g0006561

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008029
Physical Location & Seq
Forward (+)
4061706 .. 4068168
6463 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28766

Sequence Viewer

Length: 1482 bp
ATGGACCGACCCGACTCCGACTCCTCCTCCCGGCCCGTACTCTCCGACCGGGTCCTCATAAACGGCGCCGTAACGCCGGTCACGTTCTTCGCCGACGGCGTGCTGCGGTGGACGGAGAGAGGCGAGAGGAGCTTGATCGTCGAGAAGGAGGTCCTCGGGGTCAAAGTGGAGGGTCCGAAGATCAGAATCGGAGCTCTGGTCGACGGCGGAGGTGGAATCTGCTGCGTCGGCAGTAGCGGAGGAGCTCTGGTCAGGATGGACTTGGTGTTCGAGCCTTTATCAGAGGACTCGCAGAGGCTCTGGTGCCAGAAGCTTCTCGAATTCATCGATTCTCTCGGACGGCCGAAGAGGCTGTTTGTGTTTGTGAATCCATTTGGAGGGAGCAAATCGGCTTCGAAGATTTTTGTTGATGAAGTGAAGCCGCTTTTTGATGATGCTGGTGTTCAGTTTACACTGCAAGAAACTCAGTATCAACTGCATGCGAAGGAAGTTGCGAAAACTCTAGATCTTTCAAAGTATGATGGCATTGTTTGCGTCAGTGGGGATGGAATCTTGGTTGAGGTAGTAAATGGACTTCTTGAGAGAGAGGATTGGGACACTGCAATAAAGATGCCTCTCGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCAAAATCTCTTCTAGATTCTGTTGGTGAACCTTGTAAAGTATCAAATGCTGTTCTCGCCATTATTAGAGGACATAAGCGTTCATTGGATGTAGCTACCATATGGCAGGGGAAGACAAAATTTTTTAGTGTGTTGATGCTCGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTCTTGATTTCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCGCCTGGCTTCGAAGGGTTTGGAGAGCCTACTAGTTACAATTACGATGGTGAATCTACTAACAATGATCCAAGTCAAGAAAAGCCTACTAAGGTTCGACAGCTTGGCTATGAGGGACCTGATATTAATCTAAAGAACATGAACTGGAGAACAATCAAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGACACAAGGGCAGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGACGTTATAATTATGAGGGCTTGCCCAAAATTGTCCTTGCTCTCATTAATGACAGGGTTGAGCACTGGGACTCATGTCAAGTCACCATATGTGCTATACTTCAAGGTGAAAGCTTTCATTCTAGAGCCTGGTGCTCGCGCTGATGATTCAACGAAGGAAGGGATAGTGGACTCAGATGGTGAGGTCCTCGCTAGGGGCAAAGGAGCATACAAATGTCATCAGAAAACACTAATGGTGTACGATAAACTTCAAATAACTTTGGATCAAGGTTTAGCCACTCTGTTTTCCCCTTCCCCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

493

Amino Acids

54.01

Weight (kDa)

5.84

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 117 - 250 1.4e-31 Diacylglycerol kinase catalytic domain
YegS_C PF19279 366 - 470 3.3e-12 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1187
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 2 cut(s) 65, 303
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 1317
AciI CCGC 4 cut(s) 106, 207, 237, 422
AclWI GGATC 3 cut(s) 916, 989, 1449
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 2 cut(s) 320, 770
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 4 cut(s) 39, 637, 1119, 1418
AfiI CCNNNNNNNGG 6 cut(s) 30, 377, 932, 1127, 1372, 1474
AgsI TTSAA 5 cut(s) 513, 878, 1282, 1329, 1430
AhdI GACNNNNNGTC 1 cut(s) 823
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 2 cut(s) 931, 1306
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
AluBI AGCT 8 cut(s) 132, 194, 245, 313, 746, 1030, 1148, 1292
AluI AGCT 8 cut(s) 132, 194, 245, 313, 746, 1030, 1148, 1292
Alw21I GWGCWC 5 cut(s) 196, 247, 856, 1244, 1315
AlwI GGATC 3 cut(s) 916, 989, 1449
AlwNI CAGNNNCTG 2 cut(s) 638, 1151
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 32, 341
ApeKI GCWGC 3 cut(s) 103, 222, 1145
ApoI RAATTY 2 cut(s) 320, 770
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AseI ATTAAT 2 cut(s) 1053, 1226
Asp700I GAANNNNTTC 1 cut(s) 1292
AspLEI GCGC 3 cut(s) 68, 931, 1319
AspS9I GGNCC 8 cut(s) 4, 33, 52, 151, 173, 1043, 1088, 1363
AsuC2I CCSGG 3 cut(s) 31, 50, 1475
AsuHPI GGTGA 6 cut(s) 689, 989, 1142, 1254, 1297, 1370
AsuII TTCGAA 2 cut(s) 395, 939
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 7 cut(s) 4, 52, 151, 173, 1043, 1088, 1363
BanI GGYRCC 2 cut(s) 65, 303
BanII GRGCYC 2 cut(s) 196, 247
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 2 cut(s) 770, 1140
Bbv12I GWGCWC 5 cut(s) 196, 247, 856, 1244, 1315
BbvI GCAGC 3 cut(s) 90, 209, 1157
BccI CCATC 7 cut(s) 250, 515, 539, 838, 968, 1163, 1349
BceAI ACGGC 5 cut(s) 53, 79, 112, 220, 356
BciT130I CCWGG 2 cut(s) 933, 1308
BcnI CCSGG 3 cut(s) 31, 50, 1475
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 6 cut(s) 503, 665, 960, 1178, 1301, 1371
BfmI CTRYAG 1 cut(s) 630
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 349
BglII AGATCT 1 cut(s) 505
BisI GCNGC 4 cut(s) 104, 223, 422, 1146
BlsI GCNGC 4 cut(s) 105, 224, 423, 1147
Bme1390I CCNGG 5 cut(s) 31, 50, 933, 1308, 1475
Bme18I GGWCC 7 cut(s) 4, 52, 151, 173, 1043, 1088, 1363
BmeRI GACNNNNNGTC 1 cut(s) 823
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 8 cut(s) 4, 33, 52, 151, 173, 1043, 1088, 1363
BmiI GGNNCC 5 cut(s) 53, 67, 174, 305, 1044
BmrFI CCNGG 5 cut(s) 31, 50, 933, 1308, 1475
BmrI ACTGGG 1 cut(s) 1254
BmsI GCATC 4 cut(s) 424, 600, 777, 1144
BmuI ACTGGG 1 cut(s) 1254
BoxI GACNNNNGTC 1 cut(s) 1253
BpiI GAAGAC 2 cut(s) 770, 1140
BpmI CTGGAG 1 cut(s) 1093
Bpu14I TTCGAA 2 cut(s) 395, 939
BpuEI CTTGAG 1 cut(s) 599
BpuMI CCSGG 3 cut(s) 31, 50, 1475
Bsa29I ATCGAT 1 cut(s) 327
BsaBI GATNNNNATC 3 cut(s) 185, 1053, 1173
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 154, 1121, 1473
BsaXI ACNNNNNCTCC 8 cut(s) 5, 11, 35, 41, 183, 213, 234, 264
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 377, 932, 1127, 1372, 1474
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 3 cut(s) 643, 1076, 1249
Bse8I GATNNNNATC 3 cut(s) 185, 1053, 1173
BseBI CCWGG 2 cut(s) 933, 1308
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 3 cut(s) 154, 1121, 1473
BseGI GGATG 4 cut(s) 261, 550, 745, 849
BseJI GATNNNNATC 3 cut(s) 185, 1053, 1173
BseLI CCNNNNNNNGG 6 cut(s) 30, 377, 932, 1127, 1372, 1474
BseMII CTCAG 3 cut(s) 479, 1179, 1365
BseNI ACTGG 3 cut(s) 643, 1076, 1249
BseRI GAGGAG 4 cut(s) 13, 16, 142, 255
BseX3I CGGCCG 1 cut(s) 341
BseXI GCAGC 3 cut(s) 90, 209, 1157
Bsh1236I CGCG 1 cut(s) 1317
Bsh1285I CGRYCG 2 cut(s) 49, 344
BshFI GGCC 2 cut(s) 34, 343
BshNI GGYRCC 2 cut(s) 65, 303
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 2 cut(s) 49, 344
BsiHKAI GWGCWC 5 cut(s) 196, 247, 856, 1244, 1315
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 4 cut(s) 31, 49, 77, 1475
BslFI GGGAC 3 cut(s) 608, 1056, 1261
BslI CCNNNNNNNGG 6 cut(s) 30, 377, 932, 1127, 1372, 1474
BsmFI GGGAC 3 cut(s) 608, 1056, 1261
BsnI GGCC 2 cut(s) 34, 343
BsoBI CYCGRG 1 cut(s) 155
Bsp119I TTCGAA 2 cut(s) 395, 939
Bsp1286I GDGCHC 5 cut(s) 196, 247, 856, 1244, 1315
Bsp143I GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
BspACI CCGC 4 cut(s) 106, 207, 237, 422
BspANI GGCC 2 cut(s) 34, 343
BspCNI CTCAG 3 cut(s) 478, 1178, 1364
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 1 cut(s) 1317
BspLI GGNNCC 5 cut(s) 53, 67, 174, 305, 1044
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 3 cut(s) 916, 989, 1449
BspT104I TTCGAA 2 cut(s) 395, 939
BspT107I GGYRCC 2 cut(s) 65, 303
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 3 cut(s) 643, 1076, 1249
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 3 cut(s) 154, 1121, 1473
BssMI GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 2 cut(s) 933, 1308
Bst4CI ACNGT 1 cut(s) 1092
Bst6I CTCTTC 2 cut(s) 341, 666
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 531
BstBI TTCGAA 2 cut(s) 395, 939
BstC8I GCNNGC 6 cut(s) 101, 480, 792, 927, 1201, 1315
BstDEI CTNAG 5 cut(s) 465, 802, 1017, 1165, 1351
BstENI CCTNNNNNAGG 1 cut(s) 1125
BstF5I GGATG 4 cut(s) 261, 550, 745, 849
BstFNI CGCG 1 cut(s) 1317
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 931, 1319
BstKTI GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
BstMBI GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
BstMCI CGRYCG 2 cut(s) 49, 344
BstMWI GCNNNNNNNGC 7 cut(s) 129, 228, 349, 531, 707, 895, 1154
BstNI CCWGG 2 cut(s) 933, 1308
BstNSI RCATGY 1 cut(s) 482
BstPAI GACNNNNGTC 1 cut(s) 1253
BstSCI CCNGG 5 cut(s) 29, 48, 931, 1306, 1473
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 1 cut(s) 1317
BstV1I GCAGC 3 cut(s) 90, 209, 1157
BstV2I GAAGAC 2 cut(s) 770, 1140
BstX2I RGATCY 2 cut(s) 505, 908
BstYI RGATCY 2 cut(s) 505, 908
BstZI CGGCCG 1 cut(s) 341
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 2 cut(s) 34, 343
BsuTUI ATCGAT 1 cut(s) 327
BtsCI GGATG 4 cut(s) 261, 550, 745, 849
BtsI GCAGTG 2 cut(s) 452, 597
BtsIMutI CAGTG 5 cut(s) 452, 544, 597, 1107, 1242
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 6 cut(s) 101, 480, 792, 927, 1201, 1315
CaiI CAGNNNCTG 2 cut(s) 638, 1151
CfoI GCGC 3 cut(s) 68, 931, 1319
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 8 cut(s) 4, 33, 52, 151, 173, 1043, 1088, 1363
ClaI ATCGAT 1 cut(s) 327
CseI GACGC 2 cut(s) 214, 523
Csp6I GTAC 4 cut(s) 38, 636, 1118, 1417
CviAII CATG 4 cut(s) 479, 795, 1066, 1253
CviQI GTAC 4 cut(s) 38, 636, 1118, 1417
DdeI CTNAG 5 cut(s) 465, 802, 1017, 1165, 1351
DinI GGCGCC 1 cut(s) 67
DpnI GATC 6 cut(s) 137, 182, 507, 910, 996, 1443
DpnII GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
DriI GACNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 341
EagI CGGCCG 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 341, 666
Eam1105I GACNNNNNGTC 1 cut(s) 823
EarI CTCTTC 2 cut(s) 341, 666
EciI GGCGGA 1 cut(s) 222
Ecl136II GAGCTC 2 cut(s) 194, 245
EclXI CGGCCG 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 1121
Eco24I GRGCYC 2 cut(s) 196, 247
Eco47I GGWCC 7 cut(s) 4, 52, 151, 173, 1043, 1088, 1363
Eco52I CGGCCG 1 cut(s) 341
Eco53kI GAGCTC 2 cut(s) 194, 245
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 2 cut(s) 194, 245
EcoNI CCTNNNNNAGG 1 cut(s) 1125
EcoO109I RGGNCCY 4 cut(s) 52, 151, 1043, 1363
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 931, 1306
EcoT14I CCWWGG 1 cut(s) 1121
EcoT38I GRGCYC 2 cut(s) 196, 247
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 1121
FaeI CATG 4 cut(s) 482, 798, 1069, 1256
FaqI GGGAC 3 cut(s) 608, 1056, 1261
FatI CATG 4 cut(s) 478, 794, 1065, 1252
FauNDI CATATG 2 cut(s) 752, 1267
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 104, 223, 422, 1146
FokI GGATG 4 cut(s) 268, 557, 752, 856
FriOI GRGCYC 2 cut(s) 196, 247
Fsp4HI GCNGC 4 cut(s) 104, 223, 422, 1146
FspBI CTAG 6 cut(s) 503, 665, 960, 1178, 1301, 1371
GlaI GCGC 3 cut(s) 67, 930, 1318
GluI GCNGC 4 cut(s) 104, 223, 422, 1146
GsuI CTGGAG 1 cut(s) 1093
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 34, 343
HapII CCGG 4 cut(s) 31, 49, 77, 1475
HgaI GACGC 2 cut(s) 214, 523
HhaI GCGC 3 cut(s) 68, 931, 1319
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 4 cut(s) 482, 798, 1069, 1256
Hin6I GCGC 3 cut(s) 66, 929, 1317
HinP1I GCGC 3 cut(s) 66, 929, 1317
HincII GTYRAC 2 cut(s) 202, 817
HindII GTYRAC 2 cut(s) 202, 817
HindIII AAGCTT 2 cut(s) 311, 1290
HpaII CCGG 4 cut(s) 31, 49, 77, 1475
HphI GGTGA 6 cut(s) 689, 989, 1142, 1254, 1297, 1370
Hpy166II GTNNAC 7 cut(s) 111, 202, 450, 680, 817, 1348, 1417
Hpy8I GTNNAC 7 cut(s) 111, 202, 450, 680, 817, 1348, 1417
Hpy99I CGWCG 4 cut(s) 98, 143, 206, 230
HpyAV CCTTC 6 cut(s) 139, 478, 935, 1327, 1331, 1479
HpyCH4III ACNGT 1 cut(s) 1092
HpyCH4IV ACGT 2 cut(s) 83, 1182
HpyCH4V TGCA 5 cut(s) 457, 478, 602, 632, 889
HpyF10VI GCNNNNNNNGC 7 cut(s) 129, 228, 349, 531, 707, 895, 1154
HpyF3I CTNAG 5 cut(s) 465, 802, 1017, 1165, 1351
HpySE526I ACGT 2 cut(s) 83, 1182
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 4 cut(s) 482, 798, 1069, 1256
HspAI GCGC 3 cut(s) 66, 929, 1317
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
LmnI GCTCC 6 cut(s) 129, 191, 242, 381, 1153, 1382
Lsp1109I GCAGC 3 cut(s) 90, 209, 1157
LweI GCATC 4 cut(s) 424, 600, 777, 1144
MaeI CTAG 6 cut(s) 503, 665, 960, 1178, 1301, 1371
MaeII ACGT 2 cut(s) 83, 1182
MaeIII GTNAC 4 cut(s) 70, 79, 962, 1260
MalI GATC 6 cut(s) 137, 182, 507, 910, 996, 1443
MboI GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
MboII GAAGA 7 cut(s) 79, 190, 358, 409, 653, 775, 1145
MflI RGATCY 2 cut(s) 505, 908
MhlI GDGCHC 5 cut(s) 196, 247, 856, 1244, 1315
MluCI AATT 6 cut(s) 320, 770, 882, 967, 1188, 1208
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 6 cut(s) 8, 14, 281, 833, 1243, 1343
MmeI TCCRAC 2 cut(s) 42, 69
MroXI GAANNNNTTC 1 cut(s) 1292
MseI TTAA 2 cut(s) 1053, 1226
MslI CAYNNNNRTG 1 cut(s) 1390
MspI CCGG 4 cut(s) 31, 49, 77, 1475
MspR9I CCNGG 5 cut(s) 31, 50, 933, 1308, 1475
MvaI CCWGG 2 cut(s) 933, 1308
MvnI CGCG 1 cut(s) 1317
MwoI GCNNNNNNNGC 7 cut(s) 129, 228, 349, 531, 707, 895, 1154
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 3 cut(s) 31, 50, 1475
NdeI CATATG 2 cut(s) 752, 1267
NdeII GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
NlaIII CATG 4 cut(s) 482, 798, 1069, 1256
NlaIV GGNNCC 5 cut(s) 53, 67, 174, 305, 1044
NmuCI GTSAC 2 cut(s) 79, 1260
NspI RCATGY 1 cut(s) 482
NspV TTCGAA 2 cut(s) 395, 939
PaeI GCATGC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 3 cut(s) 80, 96, 324
PdmI GAANNNNTTC 1 cut(s) 1292
PfeI GAWTC 8 cut(s) 186, 216, 329, 367, 549, 668, 980, 1325
PflFI GACNNNGTC 1 cut(s) 50
PkrI GCNGC 4 cut(s) 105, 224, 423, 1147
PleI GAGTC 6 cut(s) 8, 14, 281, 832, 1243, 1343
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 6 cut(s) 8, 14, 281, 832, 1243, 1343
PpuMI RGGWCCY 4 cut(s) 52, 151, 1043, 1363
PshAI GACNNNNGTC 1 cut(s) 1253
PshBI ATTAAT 2 cut(s) 1053, 1226
PsiI TTATAA 1 cut(s) 1187
Psp124BI GAGCTC 2 cut(s) 196, 247
Psp5II RGGWCCY 4 cut(s) 52, 151, 1043, 1363
Psp6I CCWGG 2 cut(s) 931, 1306
PspGI CCWGG 2 cut(s) 931, 1306
PspN4I GGNNCC 5 cut(s) 53, 67, 174, 305, 1044
PspPI GGNCC 8 cut(s) 4, 33, 52, 151, 173, 1043, 1088, 1363
PspPPI RGGWCCY 4 cut(s) 52, 151, 1043, 1363
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 2 cut(s) 638, 1151
PsuI RGATCY 2 cut(s) 505, 908
PsyI GACNNNGTC 1 cut(s) 50
RsaI GTAC 4 cut(s) 39, 637, 1119, 1418
RsaNI GTAC 4 cut(s) 38, 636, 1118, 1417
RseI CAYNNNNRTG 1 cut(s) 1390
SacI GAGCTC 2 cut(s) 196, 247
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 1053, 1226
SatI GCNGC 4 cut(s) 104, 223, 422, 1146
Sau3AI GATC 6 cut(s) 135, 180, 505, 908, 994, 1441
Sau96I GGNCC 8 cut(s) 4, 33, 52, 151, 173, 1043, 1088, 1363
SbfI CCTGCAGG 1 cut(s) 634
SchI GAGTC 6 cut(s) 8, 14, 281, 833, 1243, 1343
ScrFI CCNGG 5 cut(s) 31, 50, 933, 1308, 1475
SdaI CCTGCAGG 1 cut(s) 634
SduI GDGCHC 5 cut(s) 196, 247, 856, 1244, 1315
SfaNI GCATC 4 cut(s) 424, 600, 777, 1144
SfcI CTRYAG 1 cut(s) 630
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 2 cut(s) 395, 939
SinI GGWCC 7 cut(s) 4, 52, 151, 173, 1043, 1088, 1363
SmiMI CAYNNNNRTG 1 cut(s) 1390
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 1 cut(s) 959
SphI GCATGC 1 cut(s) 482
Sse8387I CCTGCAGG 1 cut(s) 634
Sse9I AATT 6 cut(s) 320, 770, 882, 967, 1188, 1208
SsiI CCGC 4 cut(s) 106, 207, 237, 422
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 6 cut(s) 503, 665, 960, 1178, 1301, 1371
SstI GAGCTC 2 cut(s) 196, 247
StyD4I CCNGG 5 cut(s) 29, 48, 931, 1306, 1473
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 1 cut(s) 1092
TaiI ACGT 2 cut(s) 86, 1185
TaqI TCGA 8 cut(s) 141, 201, 270, 318, 327, 395, 939, 1024
TaqII GACCGA 1 cut(s) 21
TasI AATT 6 cut(s) 320, 770, 882, 967, 1188, 1208
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 8 cut(s) 186, 216, 329, 367, 549, 668, 980, 1325
Tru1I TTAA 2 cut(s) 1053, 1226
Tru9I TTAA 2 cut(s) 1053, 1226
TscAI CASTG 5 cut(s) 459, 544, 604, 1107, 1249
TseFI GTSAC 2 cut(s) 79, 1260
TseI GCWGC 3 cut(s) 103, 222, 1145
Tsp45I GTSAC 2 cut(s) 79, 1260
TspDTI ATGAA 5 cut(s) 313, 426, 723, 1082, 1285
TspGWI ACGGA 2 cut(s) 128, 921
TspRI CASTG 5 cut(s) 459, 544, 604, 1107, 1249
Tth111I GACNNNGTC 1 cut(s) 50
VpaK11BI GGWCC 7 cut(s) 4, 52, 151, 173, 1043, 1088, 1363
VspI ATTAAT 2 cut(s) 1053, 1226
XagI CCTNNNNNAGG 1 cut(s) 1125
XapI RAATTY 2 cut(s) 320, 770
XbaI TCTAGA 4 cut(s) 502, 664, 1177, 1300
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 1292
XspI CTAG 6 cut(s) 503, 665, 960, 1178, 1301, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.