Rroxscaffold_1G00031830

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008029
Physical Location & Seq
Forward (+)
45110156 .. 45112594
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031830.1

Sequence Viewer

Length: 414 bp
ATGAACTGGAGAACAATTAAAGGACTGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGACACAAGGGCAGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGATGTTATAATTATGAGGGCTTGCCTGAAATTATCCTTGCTCTCGTTAATGACTGGGTTGAGCACTGGGACTCATGTCAAGTCACCATATGTGCTATACTTCAAGGTAAAAGCATTTATTCTGGAGCCTGGTGCTCGTGCTGAGGATCCAATGAAGGAAGGGATAGAAGACTCAGATGGTGAGGTCCTCGTTAGAGGCAAAGGAGCATACAAATCTGATCAGAAGACACTAGTGGTGTATGATAAACTTCAAATAACTCTGGACCAAGGTTTATCCACTCTGTTTTCCCCCCTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

137

Amino Acids

15.21

Weight (kDa)

6.72

Isoelectric Point (pI)

24.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YegS_C PF19279 12 - 101 8.7e-12 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
AclWI GGATC 2 cut(s) 254, 267
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 2 cut(s) 217, 365
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw21I GWGCWC 2 cut(s) 179, 250
AlwI GGATC 2 cut(s) 254, 267
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 298, 376
AsuHPI GGTGA 3 cut(s) 77, 189, 305
AvaII GGWCC 2 cut(s) 298, 376
BamHI GGATCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 249
BbsI GAAGAC 3 cut(s) 75, 288, 344
Bbv12I GWGCWC 2 cut(s) 179, 250
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 92
BccI CCATC 2 cut(s) 98, 284
BciT130I CCWGG 1 cut(s) 243
BclI TGATCA 1 cut(s) 331
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 2 cut(s) 113, 344
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 243
Bme18I GGWCC 2 cut(s) 298, 376
BmgT120I GGNCC 2 cut(s) 298, 376
BmiI GGNNCC 2 cut(s) 240, 261
BmrFI CCNGG 1 cut(s) 243
BmrI ACTGGG 2 cut(s) 177, 189
BmsI GCATC 1 cut(s) 79
BmuI ACTGGG 2 cut(s) 177, 189
BoxI GACNNNNGTC 1 cut(s) 188
BpiI GAAGAC 3 cut(s) 75, 288, 344
BpmI CTGGAG 2 cut(s) 28, 257
Bpu10I CCTNAGC 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 56, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 3 cut(s) 11, 172, 184
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 2 cut(s) 56, 379
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMII CTCAG 3 cut(s) 114, 246, 300
BseNI ACTGG 3 cut(s) 11, 172, 184
BseXI GCAGC 1 cut(s) 92
BsiHKAI GWGCWC 2 cut(s) 179, 250
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 196
Bsp1286I GDGCHC 2 cut(s) 179, 250
Bsp143I GATC 2 cut(s) 259, 331
BspCNI CTCAG 3 cut(s) 113, 247, 299
BspLI GGNNCC 2 cut(s) 240, 261
BspPI GGATC 2 cut(s) 254, 267
BsrI ACTGG 3 cut(s) 11, 172, 184
BssECI CCNNGG 2 cut(s) 56, 379
BssMI GATC 2 cut(s) 259, 331
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 2 cut(s) 56, 379
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 3 cut(s) 100, 255, 286
BstENI CCTNNNNNAGG 1 cut(s) 60
BstKTI GATC 2 cut(s) 262, 334
BstMBI GATC 2 cut(s) 259, 331
BstMWI GCNNNNNNNGC 1 cut(s) 89
BstNI CCWGG 1 cut(s) 243
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 92
BstV2I GAAGAC 3 cut(s) 75, 288, 344
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 42, 177
Cac8I GCNNGC 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 298, 376
Csp6I GTAC 1 cut(s) 53
CviAII CATG 1 cut(s) 188
CviJI RGCY 4 cut(s) 43, 83, 134, 241
CviKI_1 RGCY 4 cut(s) 43, 83, 134, 241
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 3 cut(s) 100, 255, 286
DpnI GATC 2 cut(s) 261, 333
DpnII GATC 2 cut(s) 259, 331
Eco130I CCWWGG 2 cut(s) 56, 379
Eco47I GGWCC 2 cut(s) 298, 376
EcoNI CCTNNNNNAGG 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 56, 379
ErhI CCWWGG 2 cut(s) 56, 379
FaeI CATG 1 cut(s) 191
FaiI YATR 8 cut(s) 122, 128, 189, 202, 204, 211, 322, 354
FaqI GGGAC 1 cut(s) 196
FatI CATG 1 cut(s) 187
FauNDI CATATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 81
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 113, 344
GluI GCNGC 1 cut(s) 81
GsuI CTGGAG 2 cut(s) 28, 257
Hin1II CATG 1 cut(s) 191
HinfI GANTC 2 cut(s) 184, 284
HphI GGTGA 3 cut(s) 77, 189, 305
Hpy188I TCNGA 4 cut(s) 103, 289, 331, 336
Hpy188III TCNNGA 4 cut(s) 86, 113, 236, 374
HpyAV CCTTC 2 cut(s) 262, 266
HpyCH4III ACNGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 1 cut(s) 89
HpyF3I CTNAG 3 cut(s) 100, 255, 286
Hsp92II CATG 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 2 cut(s) 259, 331
LmnI GCTCC 3 cut(s) 88, 238, 317
LpnPI CCDG 8 cut(s) 99, 152, 153, 165, 221, 228, 255, 359
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 1 cut(s) 79
MaeI CTAG 2 cut(s) 113, 344
MaeIII GTNAC 1 cut(s) 195
MalI GATC 2 cut(s) 261, 333
MboI GATC 2 cut(s) 259, 331
MboII GAAGA 3 cut(s) 80, 293, 349
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 2 cut(s) 179, 250
MluCI AATT 3 cut(s) 15, 123, 143
MlyI GAGTC 2 cut(s) 178, 278
MnlI CCTC 6 cut(s) 56, 123, 250, 289, 302, 311
MseI TTAA 2 cut(s) 18, 161
MspR9I CCNGG 1 cut(s) 243
MvaI CCWGG 1 cut(s) 243
MwoI GCNNNNNNNGC 1 cut(s) 89
NdeI CATATG 1 cut(s) 202
NdeII GATC 2 cut(s) 259, 331
NlaIII CATG 1 cut(s) 191
NlaIV GGNNCC 2 cut(s) 240, 261
NmuCI GTSAC 1 cut(s) 195
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 2 cut(s) 178, 278
PpsI GAGTC 2 cut(s) 178, 278
PpuMI RGGWCCY 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 188
PsiI TTATAA 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 240, 261
PspPI GGNCC 2 cut(s) 298, 376
PspPPI RGGWCCY 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 18, 161
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 2 cut(s) 259, 331
Sau96I GGNCC 2 cut(s) 298, 376
SchI GAGTC 2 cut(s) 178, 278
ScrFI CCNGG 1 cut(s) 243
SduI GDGCHC 2 cut(s) 179, 250
SetI ASST 6 cut(s) 58, 67, 85, 222, 300, 385
SfaNI GCATC 1 cut(s) 79
SinI GGWCC 2 cut(s) 298, 376
SpeI ACTAGT 1 cut(s) 343
Sse9I AATT 3 cut(s) 15, 123, 143
SspMI CTAG 2 cut(s) 113, 344
StyD4I CCNGG 1 cut(s) 241
StyI CCWWGG 2 cut(s) 56, 379
TaaI ACNGT 1 cut(s) 27
TasI AATT 3 cut(s) 15, 123, 143
Tru1I TTAA 2 cut(s) 18, 161
Tru9I TTAA 2 cut(s) 18, 161
TscAI CASTG 2 cut(s) 42, 184
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 2 cut(s) 17, 281
TspRI CASTG 2 cut(s) 42, 184
VpaK11BI GGWCC 2 cut(s) 298, 376
XagI CCTNNNNNAGG 1 cut(s) 60
XbaI TCTAGA 1 cut(s) 112
XspI CTAG 2 cut(s) 113, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.