RLG00000031399

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008029
Physical Location & Seq
Forward (+)
4776071 .. 4780725
4655 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031399

Sequence Viewer

Length: 1455 bp
ATGGACCGACCCGACTCCTCCGACTCCTCCTCCCGGCCCGTACTCTCCGACCGGGTCCTCATAAACGGCGCCGTAACGCCGGTCACGTTCTTCGCCGACGGTGTGCTGCGTTGGACGGAGAGAGGCGAGAGGAGCTTGATCGTCGAGAAGGAGGTCCTCGGGGTCAAAGTGGAGGGTCCGAAGATCAGAATCGGAGCTCTGGTCGACGGCGGAGGTGGAATCTGCTGCGTCGGCAGTAGCGGAGGAGGTCTGGTCAGGAAGGACTTGGTGTTCGAGCCTTTATCAGAGGACTCGCAGAGGCTCTGGTGCCAGAAGCTTCTCGAATTCATCGATTCTCTCGGACGGCCGAAGAGGCTGTTTGTGTTTGTGAATCCATTTGGAGGGAGCAAATCGGCTTCGAAGATTTTTGTTGATGAAGTGAAGCCGCTTTTTGATGATGCTGGTGTTCAGTTTACACTGCAAGAAACTCAGTATCAACTGCATGCGAAGGAAGTTGCGAAAACTCTAGATCTTTCAAAGTATGATGGCATTGTTTGCGTCAGTGGGGATGGAATCTTGGTTGAGGTAGTAAATGGACTTCTTGAGAGAGAGGATTGGGACACTGCAATAAAGATGCCTCTCGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCGAAATCTCTTCTAGATTCTGTTGGTGAACCTTGTAAAGTATCAAATGCTGTTCTTGCCATTATTAGAGGACATAAGCGTTCATTGGATGTAGCTACCATATGGCAGGGGAAGACAAAATTTTTCAGTGTGTTGATGCTCGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTCTAGATTTCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCGCCTGGCTTCGAAGGGTTTGGAGAGCCTACTAGTTACAATGACGATGGTGAATCTACTAACACTGATCCAAGTCAAGAAAAGCCTACTAAGGTTCGACAGCTTGGCTATGAGGGACCTGATATTAATCTAAAGAACATGAACTGGAGAACAATCAAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGGAGTGAAGACACAAGGGCAGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGACGTTATAATTATGAGGGCTTGCCCAAAATTGTCCTTGCTCTCGTTAATGACGGGTTTGAGCACTGGGACTCATGTCAATTCACCATATGTGCTATACTTCAAGGTGAAAGCTTTCATTCTAGAGCCTGGTGCTCGCGCTGATGATTCAACGAAGGAAGGGATAGTGGACTCAGATGGTGAGGTCCTTGCTAGAGGCAAAGGAGCATACAAATGTCATCAGAAAACACTAATGGTGTACGATAAACTTCAAATAACTATGGATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

485

Amino Acids

53.1

Weight (kDa)

5.6

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 118 - 251 1.4e-31 Diacylglycerol kinase catalytic domain
YegS_C PF19279 379 - 471 5.9e-11 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 626
AccB1I GGYRCC 2 cut(s) 68, 306
AccI GTMKAC 1 cut(s) 204
AccII CGCG 1 cut(s) 1320
AciI CCGC 3 cut(s) 210, 240, 425
AclWI GGATC 3 cut(s) 919, 992, 1452
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 2 cut(s) 323, 773
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 4 cut(s) 42, 640, 1122, 1421
AfiI CCNNNNNNNGG 3 cut(s) 33, 380, 935
AgsI TTSAA 5 cut(s) 516, 881, 1285, 1332, 1433
AhdI GACNNNNNGTC 1 cut(s) 826
AhlI ACTAGT 1 cut(s) 962
AjnI CCWGG 2 cut(s) 934, 1309
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 7 cut(s) 135, 197, 316, 749, 1033, 1151, 1295
AluI AGCT 7 cut(s) 135, 197, 316, 749, 1033, 1151, 1295
Alw21I GWGCWC 4 cut(s) 199, 859, 1247, 1318
AlwI GGATC 3 cut(s) 919, 992, 1452
AlwNI CAGNNNCTG 2 cut(s) 641, 1154
Ama87I CYCGRG 1 cut(s) 158
AoxI GGCC 2 cut(s) 35, 344
ApeKI GCWGC 3 cut(s) 106, 225, 1148
ApoI RAATTY 2 cut(s) 323, 773
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AseI ATTAAT 1 cut(s) 1056
Asp700I GAANNNNTTC 1 cut(s) 1295
AspLEI GCGC 3 cut(s) 71, 934, 1322
AspS9I GGNCC 8 cut(s) 4, 36, 55, 154, 176, 1046, 1091, 1366
AsuC2I CCSGG 2 cut(s) 34, 53
AsuHPI GGTGA 5 cut(s) 692, 992, 1257, 1300, 1373
AsuII TTCGAA 2 cut(s) 398, 942
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 7 cut(s) 4, 55, 154, 176, 1046, 1091, 1366
BanI GGYRCC 2 cut(s) 68, 306
BanII GRGCYC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 561, 593
BbsI GAAGAC 2 cut(s) 773, 1143
Bbv12I GWGCWC 4 cut(s) 199, 859, 1247, 1318
BbvI GCAGC 3 cut(s) 93, 212, 1160
BccI CCATC 6 cut(s) 518, 542, 841, 971, 1166, 1352
BceAI ACGGC 4 cut(s) 56, 82, 223, 359
BciT130I CCWGG 2 cut(s) 936, 1311
BcnI CCSGG 2 cut(s) 34, 53
BcuI ACTAGT 1 cut(s) 962
BfaI CTAG 7 cut(s) 506, 668, 863, 963, 1181, 1304, 1374
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 626
BglI GCCNNNNNGGC 1 cut(s) 352
BglII AGATCT 1 cut(s) 508
BisI GCNGC 4 cut(s) 107, 226, 425, 1149
BlsI GCNGC 4 cut(s) 108, 227, 426, 1150
Bme1390I CCNGG 4 cut(s) 34, 53, 936, 1311
Bme18I GGWCC 7 cut(s) 4, 55, 154, 176, 1046, 1091, 1366
BmeRI GACNNNNNGTC 1 cut(s) 826
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 8 cut(s) 4, 36, 55, 154, 176, 1046, 1091, 1366
BmiI GGNNCC 5 cut(s) 56, 70, 177, 308, 1047
BmrFI CCNGG 4 cut(s) 34, 53, 936, 1311
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 4 cut(s) 427, 603, 780, 1147
BmuI ACTGGG 1 cut(s) 1257
BoxI GACNNNNGTC 1 cut(s) 1256
BpiI GAAGAC 2 cut(s) 773, 1143
BpmI CTGGAG 1 cut(s) 1096
Bpu14I TTCGAA 2 cut(s) 398, 942
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 2 cut(s) 34, 53
Bsa29I ATCGAT 1 cut(s) 330
BsaBI GATNNNNATC 3 cut(s) 188, 1056, 1176
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 157, 1124
BsaXI ACNNNNNCTCC 6 cut(s) 14, 44, 186, 216, 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 380, 935
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 3 cut(s) 646, 1079, 1252
Bse8I GATNNNNATC 3 cut(s) 188, 1056, 1176
BseBI CCWGG 2 cut(s) 936, 1311
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 157, 1124
BseGI GGATG 3 cut(s) 553, 748, 852
BseJI GATNNNNATC 3 cut(s) 188, 1056, 1176
BseLI CCNNNNNNNGG 3 cut(s) 33, 380, 935
BseMII CTCAG 3 cut(s) 482, 1182, 1368
BseNI ACTGG 3 cut(s) 646, 1079, 1252
BseRI GAGGAG 5 cut(s) 7, 16, 19, 145, 258
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 93, 212, 1160
Bsh1236I CGCG 1 cut(s) 1320
Bsh1285I CGRYCG 2 cut(s) 52, 347
BshFI GGCC 2 cut(s) 37, 346
BshNI GGYRCC 2 cut(s) 68, 306
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 2 cut(s) 52, 347
BsiHKAI GWGCWC 4 cut(s) 199, 859, 1247, 1318
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 3 cut(s) 34, 52, 80
BslFI GGGAC 3 cut(s) 611, 1059, 1264
BslI CCNNNNNNNGG 3 cut(s) 33, 380, 935
BsmFI GGGAC 3 cut(s) 611, 1059, 1264
BsnI GGCC 2 cut(s) 37, 346
BsoBI CYCGRG 1 cut(s) 158
Bsp119I TTCGAA 2 cut(s) 398, 942
Bsp1286I GDGCHC 4 cut(s) 199, 859, 1247, 1318
Bsp143I GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
BspACI CCGC 3 cut(s) 210, 240, 425
BspANI GGCC 2 cut(s) 37, 346
BspCNI CTCAG 3 cut(s) 481, 1181, 1367
BspDI ATCGAT 1 cut(s) 330
BspFNI CGCG 1 cut(s) 1320
BspLI GGNNCC 5 cut(s) 56, 70, 177, 308, 1047
BspMAI CTGCAG 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 626
BspPI GGATC 3 cut(s) 919, 992, 1452
BspT104I TTCGAA 2 cut(s) 398, 942
BspT107I GGYRCC 2 cut(s) 68, 306
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 3 cut(s) 646, 1079, 1252
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 2 cut(s) 157, 1124
BssMI GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 1124
Bst2UI CCWGG 2 cut(s) 936, 1311
Bst4CI ACNGT 2 cut(s) 101, 1095
Bst6I CTCTTC 2 cut(s) 344, 669
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 534
BstBI TTCGAA 2 cut(s) 398, 942
BstC8I GCNNGC 5 cut(s) 483, 795, 930, 1204, 1318
BstDEI CTNAG 5 cut(s) 468, 805, 1020, 1168, 1354
BstF5I GGATG 3 cut(s) 553, 748, 852
BstFNI CGCG 1 cut(s) 1320
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 3 cut(s) 71, 934, 1322
BstKTI GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
BstMBI GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
BstMCI CGRYCG 2 cut(s) 52, 347
BstMWI GCNNNNNNNGC 7 cut(s) 132, 231, 352, 534, 710, 898, 1157
BstNI CCWGG 2 cut(s) 936, 1311
BstNSI RCATGY 1 cut(s) 485
BstPAI GACNNNNGTC 1 cut(s) 1256
BstSCI CCNGG 4 cut(s) 32, 51, 934, 1309
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 1 cut(s) 1320
BstV1I GCAGC 3 cut(s) 93, 212, 1160
BstV2I GAAGAC 2 cut(s) 773, 1143
BstX2I RGATCY 2 cut(s) 508, 911
BstYI RGATCY 2 cut(s) 508, 911
BstZI CGGCCG 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 2 cut(s) 37, 346
BsuTUI ATCGAT 1 cut(s) 330
BtsCI GGATG 3 cut(s) 553, 748, 852
BtsI GCAGTG 2 cut(s) 455, 600
BtsIMutI CAGTG 7 cut(s) 455, 547, 600, 787, 993, 1110, 1245
BveI ACCTGC 1 cut(s) 626
Cac8I GCNNGC 5 cut(s) 483, 795, 930, 1204, 1318
CaiI CAGNNNCTG 2 cut(s) 641, 1154
CfoI GCGC 3 cut(s) 71, 934, 1322
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 8 cut(s) 4, 36, 55, 154, 176, 1046, 1091, 1366
ClaI ATCGAT 1 cut(s) 330
CseI GACGC 2 cut(s) 217, 526
Csp6I GTAC 4 cut(s) 41, 639, 1121, 1420
CviAII CATG 4 cut(s) 482, 798, 1069, 1256
CviQI GTAC 4 cut(s) 41, 639, 1121, 1420
DdeI CTNAG 5 cut(s) 468, 805, 1020, 1168, 1354
DinI GGCGCC 1 cut(s) 70
DpnI GATC 6 cut(s) 140, 185, 510, 913, 999, 1446
DpnII GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
DriI GACNNNNNGTC 1 cut(s) 826
EaeI YGGCCR 1 cut(s) 344
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 344, 669
Eam1105I GACNNNNNGTC 1 cut(s) 826
EarI CTCTTC 2 cut(s) 344, 669
EciI GGCGGA 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 197
EclXI CGGCCG 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 1124
Eco24I GRGCYC 1 cut(s) 199
Eco47I GGWCC 7 cut(s) 4, 55, 154, 176, 1046, 1091, 1366
Eco52I CGGCCG 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 197
EcoO109I RGGNCCY 4 cut(s) 55, 154, 1046, 1366
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 934, 1309
EcoT14I CCWWGG 1 cut(s) 1124
EcoT38I GRGCYC 1 cut(s) 199
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 1124
FaeI CATG 4 cut(s) 485, 801, 1072, 1259
FaqI GGGAC 3 cut(s) 611, 1059, 1264
FatI CATG 4 cut(s) 481, 797, 1068, 1255
FauNDI CATATG 2 cut(s) 755, 1270
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 4 cut(s) 107, 226, 425, 1149
FokI GGATG 3 cut(s) 560, 755, 859
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 4 cut(s) 107, 226, 425, 1149
FspBI CTAG 7 cut(s) 506, 668, 863, 963, 1181, 1304, 1374
GlaI GCGC 3 cut(s) 70, 933, 1321
GluI GCNGC 4 cut(s) 107, 226, 425, 1149
GsuI CTGGAG 1 cut(s) 1096
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 2 cut(s) 37, 346
HapII CCGG 3 cut(s) 34, 52, 80
HgaI GACGC 2 cut(s) 217, 526
HhaI GCGC 3 cut(s) 71, 934, 1322
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 4 cut(s) 485, 801, 1072, 1259
Hin6I GCGC 3 cut(s) 69, 932, 1320
HinP1I GCGC 3 cut(s) 69, 932, 1320
HincII GTYRAC 2 cut(s) 205, 820
HindII GTYRAC 2 cut(s) 205, 820
HindIII AAGCTT 2 cut(s) 314, 1293
HpaII CCGG 3 cut(s) 34, 52, 80
HphI GGTGA 5 cut(s) 692, 992, 1257, 1300, 1373
Hpy166II GTNNAC 6 cut(s) 205, 453, 683, 820, 1351, 1420
Hpy8I GTNNAC 6 cut(s) 205, 453, 683, 820, 1351, 1420
Hpy99I CGWCG 4 cut(s) 101, 146, 209, 233
HpyAV CCTTC 6 cut(s) 142, 253, 481, 938, 1330, 1334
HpyCH4III ACNGT 2 cut(s) 101, 1095
HpyCH4IV ACGT 2 cut(s) 86, 1185
HpyCH4V TGCA 5 cut(s) 460, 481, 605, 635, 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 231, 352, 534, 710, 898, 1157
HpyF3I CTNAG 5 cut(s) 468, 805, 1020, 1168, 1354
HpySE526I ACGT 2 cut(s) 86, 1185
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 4 cut(s) 485, 801, 1072, 1259
HspAI GCGC 3 cut(s) 69, 932, 1320
KasI GGCGCC 1 cut(s) 68
Kzo9I GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
LmnI GCTCC 5 cut(s) 132, 194, 384, 1156, 1385
Lsp1109I GCAGC 3 cut(s) 93, 212, 1160
LweI GCATC 4 cut(s) 427, 603, 780, 1147
MaeI CTAG 7 cut(s) 506, 668, 863, 963, 1181, 1304, 1374
MaeII ACGT 2 cut(s) 86, 1185
MaeIII GTNAC 3 cut(s) 73, 82, 965
MalI GATC 6 cut(s) 140, 185, 510, 913, 999, 1446
MboI GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
MboII GAAGA 7 cut(s) 82, 193, 361, 412, 656, 778, 1148
MflI RGATCY 2 cut(s) 508, 911
MhlI GDGCHC 4 cut(s) 199, 859, 1247, 1318
MluCI AATT 6 cut(s) 323, 773, 885, 1191, 1211, 1261
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 6 cut(s) 8, 17, 284, 836, 1246, 1346
MmeI TCCRAC 3 cut(s) 45, 72, 92
MroXI GAANNNNTTC 1 cut(s) 1295
MseI TTAA 2 cut(s) 1056, 1229
MslI CAYNNNNRTG 1 cut(s) 1393
MspI CCGG 3 cut(s) 34, 52, 80
MspR9I CCNGG 4 cut(s) 34, 53, 936, 1311
MvaI CCWGG 2 cut(s) 936, 1311
MvnI CGCG 1 cut(s) 1320
MwoI GCNNNNNNNGC 7 cut(s) 132, 231, 352, 534, 710, 898, 1157
NarI GGCGCC 1 cut(s) 69
NciI CCSGG 2 cut(s) 34, 53
NdeI CATATG 2 cut(s) 755, 1270
NdeII GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
NlaIII CATG 4 cut(s) 485, 801, 1072, 1259
NlaIV GGNNCC 5 cut(s) 56, 70, 177, 308, 1047
NmuCI GTSAC 1 cut(s) 82
NspI RCATGY 1 cut(s) 485
NspV TTCGAA 2 cut(s) 398, 942
PaeI GCATGC 1 cut(s) 485
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 327
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 8 cut(s) 189, 219, 332, 370, 552, 671, 983, 1328
PflFI GACNNNGTC 1 cut(s) 53
PkrI GCNGC 4 cut(s) 108, 227, 426, 1150
PleI GAGTC 6 cut(s) 8, 17, 284, 835, 1246, 1346
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 6 cut(s) 8, 17, 284, 835, 1246, 1346
PpuMI RGGWCCY 4 cut(s) 55, 154, 1046, 1366
PshAI GACNNNNGTC 1 cut(s) 1256
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 199
Psp5II RGGWCCY 4 cut(s) 55, 154, 1046, 1366
Psp6I CCWGG 2 cut(s) 934, 1309
PspGI CCWGG 2 cut(s) 934, 1309
PspN4I GGNNCC 5 cut(s) 56, 70, 177, 308, 1047
PspPI GGNCC 8 cut(s) 4, 36, 55, 154, 176, 1046, 1091, 1366
PspPPI RGGWCCY 4 cut(s) 55, 154, 1046, 1366
PstI CTGCAG 1 cut(s) 637
PstNI CAGNNNCTG 2 cut(s) 641, 1154
PsuI RGATCY 2 cut(s) 508, 911
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 4 cut(s) 42, 640, 1122, 1421
RsaNI GTAC 4 cut(s) 41, 639, 1121, 1420
RseI CAYNNNNRTG 1 cut(s) 1393
SacI GAGCTC 1 cut(s) 199
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 2 cut(s) 1056, 1229
SatI GCNGC 4 cut(s) 107, 226, 425, 1149
Sau3AI GATC 6 cut(s) 138, 183, 508, 911, 997, 1444
Sau96I GGNCC 8 cut(s) 4, 36, 55, 154, 176, 1046, 1091, 1366
SbfI CCTGCAGG 1 cut(s) 637
SchI GAGTC 6 cut(s) 8, 17, 284, 836, 1246, 1346
ScrFI CCNGG 4 cut(s) 34, 53, 936, 1311
SdaI CCTGCAGG 1 cut(s) 637
SduI GDGCHC 4 cut(s) 199, 859, 1247, 1318
SfaNI GCATC 4 cut(s) 427, 603, 780, 1147
SfcI CTRYAG 1 cut(s) 633
SfoI GGCGCC 1 cut(s) 70
SfuI TTCGAA 2 cut(s) 398, 942
SinI GGWCC 7 cut(s) 4, 55, 154, 176, 1046, 1091, 1366
SmiMI CAYNNNNRTG 1 cut(s) 1393
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SpeI ACTAGT 1 cut(s) 962
SphI GCATGC 1 cut(s) 485
Sse8387I CCTGCAGG 1 cut(s) 637
Sse9I AATT 6 cut(s) 323, 773, 885, 1191, 1211, 1261
SsiI CCGC 3 cut(s) 210, 240, 425
SspDI GGCGCC 1 cut(s) 68
SspMI CTAG 7 cut(s) 506, 668, 863, 963, 1181, 1304, 1374
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 4 cut(s) 32, 51, 934, 1309
StyI CCWWGG 1 cut(s) 1124
TaaI ACNGT 2 cut(s) 101, 1095
TaiI ACGT 2 cut(s) 89, 1188
TaqI TCGA 8 cut(s) 144, 204, 273, 321, 330, 398, 942, 1027
TaqII GACCGA 1 cut(s) 21
TasI AATT 6 cut(s) 323, 773, 885, 1191, 1211, 1261
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 8 cut(s) 189, 219, 332, 370, 552, 671, 983, 1328
Tru1I TTAA 2 cut(s) 1056, 1229
Tru9I TTAA 2 cut(s) 1056, 1229
TscAI CASTG 7 cut(s) 462, 547, 607, 787, 1000, 1110, 1252
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 3 cut(s) 106, 225, 1148
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 5 cut(s) 316, 429, 726, 1085, 1288
TspGWI ACGGA 2 cut(s) 131, 924
TspRI CASTG 7 cut(s) 462, 547, 607, 787, 1000, 1110, 1252
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 7 cut(s) 4, 55, 154, 176, 1046, 1091, 1366
VspI ATTAAT 1 cut(s) 1056
XapI RAATTY 2 cut(s) 323, 773
XbaI TCTAGA 5 cut(s) 505, 667, 862, 1180, 1303
XceI RCATGY 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 7 cut(s) 506, 668, 863, 963, 1181, 1304, 1374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.