Rorug04G0422300

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008029
Physical Location & Seq
Reverse (-)
58208198 .. 58209774
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0422300.1

Sequence Viewer

Length: 861 bp
ATGGCAGACTCAAACACCCACCTCGACTTCGCCGTCAACAACCACTTCTCCATGCTCCGCCTCGATTCCTCCTCCAAACCCCATTCCTCCGCCGCCTTGAAACGCCCCTCTCCGCCTGCTTCCTCCCACCAACCCAACCCCAAGAAAGAGAAGCTCTCTCCTCCTCCTCCCCTCTTCCCCGGCTTTTCCAAAATTACCCCTCTCCCTCCTCTCCCCAAATCCCACTCGGGTCTTGCCCGAACCCGACCCCGCAACCCGCTCCTCCGCCGCTGCATCTCCGACCCCTTCACCCCTCCTCGCCTTGATTCTCTCTCCGCTCCTCCCGTCCCCGATTCCATCCTCTCTCCCGAGAACGACGTCACTGCCATCACTTCCCCGCCGTCCGTGATCTCCACGCGTCCGCCACCCCGCCCCACGCTCCACCGCACAGTCTCCGACCCCACCTCTTCCCCCGCCCCCAAATCCGCCTCCCGCACTTCCTCCAGCTCCGGCGACGGCGACGACGATACCCCCAACACTAAGAGGAGGCTTAAGAGGATCAAAGATCGGTTCAGAGAGATGACGCTCTGGTTTGAACAAGTCATGCTTGAAGATGAAGATGATGATGAGGAAAAAGTACCAGAAATTCAACAAGAACAAGAAGAAGAGGAACAGATTATCAATACTGAACCCACTGATGACCATGTCCAAGGTCTTGAAAACACTGCAGTCACTAAGGAGGATTGTGATCAAAAGAGGTTTGAGGAATCGGTGAGTGTGGAGAAGGTTGGGGAGTGCATTGTGATCCATTTCAGCTGCCACTGTGGGGTTCCCTATCAGTTTCTTCTTGCTGGAGGTAACTGCTACTACAAGCTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

286

Amino Acids

31.82

Weight (kDa)

5.63

Isoelectric Point (pI)

70.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 32
AatII GACGTC 1 cut(s) 360
AccBSI CCGCTC 2 cut(s) 259, 317
AccII CGCG 1 cut(s) 397
AclWI GGATC 2 cut(s) 545, 778
AcsI RAATTY 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 1 cut(s) 805
AflII CTTAAG 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 395
AgsI TTSAA 5 cut(s) 100, 575, 590, 629, 698
AluBI AGCT 4 cut(s) 154, 486, 795, 853
AluI AGCT 4 cut(s) 154, 486, 795, 853
Alw26I GTCTC 1 cut(s) 436
AlwI GGATC 2 cut(s) 545, 778
Ama87I CYCGRG 2 cut(s) 226, 347
ApeKI GCWGC 2 cut(s) 270, 795
ApoI RAATTY 1 cut(s) 624
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 280, 763
AvaI CYCGRG 2 cut(s) 226, 347
BbvI GCAGC 2 cut(s) 257, 782
BccI CCATC 2 cut(s) 344, 374
BceAI ACGGC 3 cut(s) 17, 364, 511
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 436
BfmI CTRYAG 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 530
BisI GCNGC 4 cut(s) 93, 268, 271, 796
BlsI GCNGC 4 cut(s) 94, 269, 272, 797
Bme1390I CCNGG 1 cut(s) 180
BmeT110I CYCGRG 2 cut(s) 226, 347
BmiI GGNNCC 1 cut(s) 810
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 140, 172
BpmI CTGGAG 2 cut(s) 466, 852
BpuMI CCSGG 1 cut(s) 180
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 178, 688
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse8I GATNNNNATC 1 cut(s) 726
BseDI CCNNGG 2 cut(s) 178, 688
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 1 cut(s) 805
BseRI GAGGAG 9 cut(s) 61, 150, 153, 156, 198, 251, 285, 309, 538
BseXI GCAGC 2 cut(s) 257, 782
Bsh1236I CGCG 1 cut(s) 397
BsiHKCI CYCGRG 2 cut(s) 226, 347
BsiSI CCGG 2 cut(s) 180, 489
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 311
BsoBI CYCGRG 2 cut(s) 226, 347
Bsp143I GATC 5 cut(s) 387, 537, 544, 727, 783
BspFNI CGCG 1 cut(s) 397
BspLI GGNNCC 1 cut(s) 810
BspMAI CTGCAG 1 cut(s) 709
BspPI GGATC 2 cut(s) 545, 778
BspTI CTTAAG 1 cut(s) 530
BsrBI CCGCTC 2 cut(s) 259, 317
BssECI CCNNGG 2 cut(s) 178, 688
BssMI GATC 5 cut(s) 387, 537, 544, 727, 783
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 688
Bst4CI ACNGT 2 cut(s) 430, 803
Bst6I CTCTTC 3 cut(s) 179, 451, 639
BstACI GRCGYC 1 cut(s) 357
BstAFI CTTAAG 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 2 cut(s) 519, 714
BstF5I GGATG 1 cut(s) 336
BstFNI CGCG 1 cut(s) 397
BstKTI GATC 5 cut(s) 390, 540, 547, 730, 786
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 5 cut(s) 387, 537, 544, 727, 783
BstSCI CCNGG 1 cut(s) 178
BstSFI CTRYAG 1 cut(s) 705
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 2 cut(s) 257, 782
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 2 cut(s) 360, 702
BtsIMutI CAGTG 4 cut(s) 360, 672, 702, 799
Cac8I GCNNGC 1 cut(s) 117
CseI GACGC 2 cut(s) 386, 571
Csp6I GTAC 1 cut(s) 617
CviAII CATG 4 cut(s) 52, 583, 683, 856
CviJI RGCY 6 cut(s) 154, 183, 486, 529, 795, 853
CviKI_1 RGCY 6 cut(s) 154, 183, 486, 529, 795, 853
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 2 cut(s) 519, 714
DpnI GATC 5 cut(s) 389, 539, 546, 729, 785
DpnII GATC 5 cut(s) 387, 537, 544, 727, 783
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
Eam1104I CTCTTC 3 cut(s) 179, 451, 639
EarI CTCTTC 3 cut(s) 179, 451, 639
EciI GGCGGA 6 cut(s) 47, 79, 102, 254, 390, 454
Eco130I CCWWGG 1 cut(s) 688
Eco88I CYCGRG 2 cut(s) 226, 347
EcoT14I CCWWGG 1 cut(s) 688
ErhI CCWWGG 1 cut(s) 688
FaeI CATG 4 cut(s) 55, 586, 686, 859
FaiI YATR 4 cut(s) 53, 584, 684, 857
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 311
FatI CATG 4 cut(s) 51, 582, 682, 855
FauI CCCGC 6 cut(s) 257, 264, 384, 416, 460, 479
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 4 cut(s) 93, 268, 271, 796
FokI GGATG 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 93, 268, 271, 796
GluI GCNGC 4 cut(s) 93, 268, 271, 796
GsuI CTGGAG 2 cut(s) 466, 852
HapII CCGG 2 cut(s) 180, 489
HgaI GACGC 2 cut(s) 386, 571
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 4 cut(s) 55, 586, 686, 859
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 5 cut(s) 8, 65, 305, 332, 746
HpaII CCGG 2 cut(s) 180, 489
HphI GGTGA 2 cut(s) 280, 763
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 3 cut(s) 280, 436, 554
Hpy188III TCNNGA 2 cut(s) 347, 695
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 4 cut(s) 359, 497, 503, 506
HpyAV CCTTC 2 cut(s) 295, 757
HpyCH4III ACNGT 2 cut(s) 430, 803
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 3 cut(s) 273, 707, 777
HpyF3I CTNAG 2 cut(s) 519, 714
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 4 cut(s) 55, 586, 686, 859
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 5 cut(s) 387, 537, 544, 727, 783
LmnI GCTCC 5 cut(s) 60, 264, 322, 423, 491
LpnPI CCDG 7 cut(s) 129, 193, 496, 502, 553, 633, 816
Lsp1109I GCAGC 2 cut(s) 257, 782
LweI GCATC 1 cut(s) 282
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 3 cut(s) 358, 709, 836
MalI GATC 5 cut(s) 389, 539, 546, 729, 785
MbiI CCGCTC 2 cut(s) 259, 317
MboI GATC 5 cut(s) 387, 537, 544, 727, 783
MboII GAAGA 7 cut(s) 166, 438, 602, 608, 653, 656, 815
MluCI AATT 2 cut(s) 192, 624
MluI ACGCGT 1 cut(s) 395
MlyI GAGTC 1 cut(s) 2
MmeI TCCRAC 2 cut(s) 303, 459
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 270, 795
MspCI CTTAAG 1 cut(s) 530
MspI CCGG 2 cut(s) 180, 489
MspR9I CCNGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 397
NciI CCSGG 1 cut(s) 180
NdeII GATC 5 cut(s) 387, 537, 544, 727, 783
NlaIII CATG 4 cut(s) 55, 586, 686, 859
NlaIV GGNNCC 1 cut(s) 810
NmuCI GTSAC 2 cut(s) 358, 709
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 501
PfeI GAWTC 4 cut(s) 65, 305, 332, 746
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 4 cut(s) 94, 269, 272, 797
PleI GAGTC 1 cut(s) 2
PpsI GAGTC 1 cut(s) 2
PspN4I GGNNCC 1 cut(s) 810
PstI CTGCAG 1 cut(s) 709
PsyI GACNNNGTC 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 795
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 4 cut(s) 93, 268, 271, 796
Sau3AI GATC 5 cut(s) 387, 537, 544, 727, 783
SchI GAGTC 1 cut(s) 2
ScrFI CCNGG 1 cut(s) 180
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 705
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 2 cut(s) 192, 624
StyD4I CCNGG 1 cut(s) 178
StyI CCWWGG 1 cut(s) 688
TaaI ACNGT 2 cut(s) 430, 803
TaiI ACGT 1 cut(s) 360
TaqI TCGA 2 cut(s) 24, 63
TasI AATT 2 cut(s) 192, 624
TauI GCSGC 2 cut(s) 95, 270
TfiI GAWTC 4 cut(s) 65, 305, 332, 746
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 367, 679, 709, 806
TseFI GTSAC 2 cut(s) 358, 709
TseI GCWGC 2 cut(s) 270, 795
Tsp45I GTSAC 2 cut(s) 358, 709
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 4 cut(s) 367, 679, 709, 806
Tth111I GACNNNGTC 1 cut(s) 683
Vha464I CTTAAG 1 cut(s) 530
XapI RAATTY 1 cut(s) 624
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.