Rroxscaffold_1G00069500

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008029
Physical Location & Seq
Reverse (-)
90443107 .. 90448614
5508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00069500.1

Sequence Viewer

Length: 1731 bp
ATGGACCGACCCGACTCCTCCTCACAGCCTGTACTCTCCGACCGGGTCCTCATAAATGGCGCCGTGACGCCGGTCACGTTCTTCGCCGACGGAGTGCTGCGGTGGACGGAGAGAGGCGAGAGGAGATTGATCGTCGAGAAGGAGGTCCTCGGGGTCAAAGTGGAGGGTCCGAAGATCAGAATCGGAGCTCTGGTCGACGGCGGCGGTGGAATCTTCTGTGTCGGCAGTAGCGGAGGAGCTCTGGTCAGGAAGGACTTGGTGTTCGAGCCTTTATCTGAGGACTCGCAGAGGCTCTGGTGCCAGAAGCTTCTCGAATTCATCGACTCTCTCGGACGGCCGAAGAGGCTGTTTGTGTTTGTGAATCCATTTGGAGGGAGCAAATCGGCTTCGAAGATTTTTGTTGATGAAGTGAAGCCGCTTATTGATGATGCTGGTGTTCAGTTTACACTGCAAGAAACTCAGTATCAACTGCACGCGAAGGAAGTTGCGAAAACTCTAGATCTTTCGAAGTATGATGGCATTATTTGCGTCAGTGGGGATGGAATCTTGGTTGAGGGGATCAAGTCTGATATATCATTAGTAGTAAATGGACTTCTTGAGAGAGAGGATTGGGACACTGCAATAAAGATGCCTCTTGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCGAAATCTCTTCTAGATTCTGTTGGTGAACCTTGTAAAGTATCAAATGCTGTTCTCGCCATTATTAGAGGACATAAGCGTTCATTGGATGTAGCTACCATATGGCAGGGGAAGACAAAATTTTTTAGTGTGTTGATGCTCGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTCTAGATTTCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCACCTGGATTCGAAGGGTTTGGAGAGCCTACTAATTACAATGACGATGATGAATCTACTAACACTGATCCAAGTCAAGAAAAGCCTACTAAGGTTCGACAACTTGGCTATGAGGGACCTGATATTAATCTAAAGAACATGAACTGGAGAACAATCAAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGACACAAGGGCGGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGACGTTATAATTATGAGGGCTTGCCCAAAATTGTCCTTGCTCTCTTTAATGACGGGGTTGAGCACTGGGACTCATGTCAAGTCACCATATGTGCTATACTTCAAGGTGAAAGCTTTCATTCTAGAGCCTGGTGCTCGCGCTGATGATTCAACGAAGGAAGGGATAGTGGACTCAGATGGTGAGATTGAATGGCAACCTTACCAGCTGGGAAACCTGCTGAATGATTATCCTCAGCACCGTAAGAGTTTTTTATGTAGGACTATACTCATTTGCTATAACAAGGTGGCATATCATCGCCCTGATCTATGTCCAAAGCAGTATGGCATTCAGAATCCAAATTATAGTGGTCTGCGAGAATTGATAAAAGTTAAATTCAAGCAGATTAAATCTTCAGGGATCACTGACTGGTCTAGGTATAAGTCAGGGAAAAAAGTTGACTATACTAAATATGTTCAGGAGTGGAATCTCCGGGGAGAGTCCCTTTTGAAAGATGCTTTAGGAGCTCAATCTCAGCACAATCCAGCCTGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

576

Amino Acids

64.1

Weight (kDa)

7.52

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 115 - 256 1.1e-26 Diacylglycerol kinase catalytic domain
YegS_C PF19279 372 - 461 7.5e-11 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1205
Acc36I ACCTGC 2 cut(s) 641, 1419
AccB1I GGYRCC 2 cut(s) 59, 297
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 476, 1335
AciI CCGC 6 cut(s) 100, 201, 204, 231, 416, 1163
AclWI GGATC 4 cut(s) 566, 934, 1007, 1601
AcoI YGGCCR 1 cut(s) 335
AcsI RAATTY 3 cut(s) 314, 788, 1568
AcuI CTGAAG 1 cut(s) 1572
AcyI GRCGYC 2 cut(s) 60, 68
AfaI GTAC 3 cut(s) 33, 655, 1137
AfiI CCNNNNNNNGG 3 cut(s) 371, 950, 1145
AgsI TTSAA 6 cut(s) 896, 1300, 1347, 1385, 1573, 1684
AhdI GACNNNNNGTC 1 cut(s) 841
AjnI CCWGG 2 cut(s) 949, 1324
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 7 cut(s) 188, 239, 307, 764, 1310, 1402, 1700
AluI AGCT 7 cut(s) 188, 239, 307, 764, 1310, 1402, 1700
Alw21I GWGCWC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
AlwI GGATC 4 cut(s) 566, 934, 1007, 1601
AlwNI CAGNNNCTG 1 cut(s) 656
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 3 cut(s) 314, 788, 1568
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AseI ATTAAT 1 cut(s) 1071
Asp700I GAANNNNTTC 1 cut(s) 1310
AspLEI GCGC 2 cut(s) 62, 1337
AspS9I GGNCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
AsuC2I CCSGG 2 cut(s) 44, 1667
AsuHPI GGTGA 5 cut(s) 707, 1160, 1272, 1315, 1388
AsuII TTCGAA 3 cut(s) 389, 506, 957
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
BanI GGYRCC 2 cut(s) 59, 297
BanII GRGCYC 3 cut(s) 190, 241, 1702
BarI GAAGNNNNNNTAC 2 cut(s) 576, 608
BbsI GAAGAC 2 cut(s) 788, 1158
Bbv12I GWGCWC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
BbvCI CCTCAGC 1 cut(s) 1428
BbvI GCAGC 1 cut(s) 84
BccI CCATC 5 cut(s) 509, 533, 856, 1181, 1367
BceAI ACGGC 3 cut(s) 47, 214, 350
BciT130I CCWGG 2 cut(s) 951, 1326
BcnI CCSGG 2 cut(s) 44, 1667
BfaI CTAG 6 cut(s) 497, 683, 878, 1196, 1319, 1608
BfmI CTRYAG 1 cut(s) 648
BfoI RGCGCY 1 cut(s) 63
BfuAI ACCTGC 2 cut(s) 641, 1419
BglI GCCNNNNNGGC 1 cut(s) 343
BglII AGATCT 1 cut(s) 499
BisI GCNGC 4 cut(s) 98, 202, 416, 1164
BlsI GCNGC 4 cut(s) 99, 203, 417, 1165
Bme1390I CCNGG 4 cut(s) 44, 951, 1326, 1667
Bme18I GGWCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
BmeRI GACNNNNNGTC 1 cut(s) 841
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
BmiI GGNNCC 6 cut(s) 47, 61, 168, 299, 1062, 1167
BmrFI CCNGG 4 cut(s) 44, 951, 1326, 1667
BmrI ACTGGG 1 cut(s) 1272
BmsI GCATC 5 cut(s) 418, 618, 795, 1162, 1678
BmuI ACTGGG 1 cut(s) 1272
BoxI GACNNNNGTC 2 cut(s) 71, 1271
BpiI GAAGAC 2 cut(s) 788, 1158
BpmI CTGGAG 1 cut(s) 1111
Bpu10I CCTNAGC 1 cut(s) 1428
Bpu14I TTCGAA 3 cut(s) 389, 506, 957
BpuEI CTTGAG 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 44, 1667
BsaBI GATNNNNATC 3 cut(s) 179, 1071, 1191
BsaHI GRCGYC 2 cut(s) 60, 68
BsaJI CCNNGG 3 cut(s) 148, 1139, 1666
BsaXI ACNNNNNCTCC 4 cut(s) 177, 207, 228, 258
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 950, 1145
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 4 cut(s) 661, 1094, 1267, 1607
Bse8I GATNNNNATC 3 cut(s) 179, 1071, 1191
BseBI CCWGG 2 cut(s) 951, 1326
BseDI CCNNGG 3 cut(s) 148, 1139, 1666
BseGI GGATG 3 cut(s) 544, 763, 867
BseJI GATNNNNATC 3 cut(s) 179, 1071, 1191
BseLI CCNNNNNNNGG 3 cut(s) 371, 950, 1145
BseMII CTCAG 6 cut(s) 267, 473, 1197, 1383, 1442, 1721
BseNI ACTGG 4 cut(s) 661, 1094, 1267, 1607
BseRI GAGGAG 4 cut(s) 7, 10, 136, 249
BseX3I CGGCCG 1 cut(s) 335
BseXI GCAGC 1 cut(s) 84
BseYI CCCAGC 1 cut(s) 1402
BsgI GTGCAG 2 cut(s) 455, 930
Bsh1236I CGCG 2 cut(s) 476, 1335
Bsh1285I CGRYCG 2 cut(s) 43, 338
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 2 cut(s) 59, 297
BsiEI CGRYCG 2 cut(s) 43, 338
BsiHKAI GWGCWC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 43, 71, 1666
BslFI GGGAC 4 cut(s) 626, 1074, 1279, 1660
BslI CCNNNNNNNGG 3 cut(s) 371, 950, 1145
BsmFI GGGAC 4 cut(s) 626, 1074, 1279, 1660
BsmI GAATGC 1 cut(s) 1521
BsnI GGCC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 3 cut(s) 389, 506, 957
Bsp1286I GDGCHC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
Bsp143I GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
BspACI CCGC 6 cut(s) 100, 201, 204, 231, 416, 1163
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 6 cut(s) 268, 472, 1196, 1382, 1441, 1720
BspFNI CGCG 2 cut(s) 476, 1335
BspLI GGNNCC 6 cut(s) 47, 61, 168, 299, 1062, 1167
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 641, 1419
BspPI GGATC 4 cut(s) 566, 934, 1007, 1601
BspT104I TTCGAA 3 cut(s) 389, 506, 957
BspT107I GGYRCC 2 cut(s) 59, 297
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 4 cut(s) 661, 1094, 1267, 1607
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 3 cut(s) 148, 1139, 1666
BssMI GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
BssNI GRCGYC 2 cut(s) 60, 68
BssT1I CCWWGG 1 cut(s) 1139
Bst2UI CCWGG 2 cut(s) 951, 1326
Bst4CI ACNGT 2 cut(s) 1110, 1436
Bst6I CTCTTC 2 cut(s) 335, 684
BstACI GRCGYC 2 cut(s) 60, 68
BstAPI GCANNNNNTGC 1 cut(s) 525
BstBI TTCGAA 3 cut(s) 389, 506, 957
BstC8I GCNNGC 6 cut(s) 474, 810, 945, 1219, 1333, 1723
BstDEI CTNAG 8 cut(s) 276, 459, 820, 1035, 1183, 1369, 1428, 1707
BstENI CCTNNNNNAGG 1 cut(s) 1143
BstF5I GGATG 3 cut(s) 544, 763, 867
BstFNI CGCG 2 cut(s) 476, 1335
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 2 cut(s) 62, 1337
BstKTI GATC 8 cut(s) 132, 177, 502, 561, 929, 1015, 1501, 1596
BstMBI GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
BstMCI CGRYCG 2 cut(s) 43, 338
BstMWI GCNNNNNNNGC 6 cut(s) 343, 525, 725, 913, 1172, 1697
BstNI CCWGG 2 cut(s) 951, 1326
BstPAI GACNNNNGTC 2 cut(s) 71, 1271
BstSCI CCNGG 4 cut(s) 42, 949, 1324, 1665
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 2 cut(s) 476, 1335
BstV1I GCAGC 1 cut(s) 84
BstV2I GAAGAC 2 cut(s) 788, 1158
BstX2I RGATCY 2 cut(s) 499, 926
BstXI CCANNNNNNTGG 1 cut(s) 1725
BstYI RGATCY 2 cut(s) 499, 926
BstZI CGGCCG 1 cut(s) 335
BsuRI GGCC 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 1475
BtsCI GGATG 3 cut(s) 544, 763, 867
BtsI GCAGTG 2 cut(s) 446, 615
BtsIMutI CAGTG 7 cut(s) 446, 538, 615, 1008, 1125, 1260, 1596
BveI ACCTGC 2 cut(s) 641, 1419
Cac8I GCNNGC 6 cut(s) 474, 810, 945, 1219, 1333, 1723
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 2 cut(s) 62, 1337
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
CseI GACGC 2 cut(s) 76, 517
Csp6I GTAC 3 cut(s) 32, 654, 1136
CviAII CATG 3 cut(s) 813, 1084, 1271
CviQI GTAC 3 cut(s) 32, 654, 1136
DdeI CTNAG 8 cut(s) 276, 459, 820, 1035, 1183, 1369, 1428, 1707
DinI GGCGCC 1 cut(s) 61
DpnI GATC 8 cut(s) 131, 176, 501, 560, 928, 1014, 1500, 1595
DpnII GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
DriI GACNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 1 cut(s) 335
EagI CGGCCG 1 cut(s) 335
Eam1104I CTCTTC 2 cut(s) 335, 684
Eam1105I GACNNNNNGTC 1 cut(s) 841
EarI CTCTTC 2 cut(s) 335, 684
Ecl136II GAGCTC 3 cut(s) 188, 239, 1700
EclXI CGGCCG 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 1139
Eco24I GRGCYC 3 cut(s) 190, 241, 1702
Eco47I GGWCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
Eco52I CGGCCG 1 cut(s) 335
Eco53kI GAGCTC 3 cut(s) 188, 239, 1700
Eco57I CTGAAG 1 cut(s) 1572
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 3 cut(s) 188, 239, 1700
EcoNI CCTNNNNNAGG 1 cut(s) 1143
EcoO109I RGGNCCY 3 cut(s) 46, 145, 1061
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 949, 1324
EcoT14I CCWWGG 1 cut(s) 1139
EcoT38I GRGCYC 3 cut(s) 190, 241, 1702
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 1139
FaeI CATG 3 cut(s) 816, 1087, 1274
FaqI GGGAC 4 cut(s) 626, 1074, 1279, 1660
FatI CATG 3 cut(s) 812, 1083, 1270
FauNDI CATATG 2 cut(s) 770, 1285
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 4 cut(s) 98, 202, 416, 1164
FokI GGATG 3 cut(s) 551, 770, 874
FriOI GRGCYC 3 cut(s) 190, 241, 1702
Fsp4HI GCNGC 4 cut(s) 98, 202, 416, 1164
FspBI CTAG 6 cut(s) 497, 683, 878, 1196, 1319, 1608
GlaI GCGC 2 cut(s) 61, 1336
GluI GCNGC 4 cut(s) 98, 202, 416, 1164
GsaI CCCAGC 1 cut(s) 1406
GsuI CTGGAG 1 cut(s) 1111
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 1 cut(s) 337
HapII CCGG 3 cut(s) 43, 71, 1666
HgaI GACGC 2 cut(s) 76, 517
HhaI GCGC 2 cut(s) 62, 1337
Hin1I GRCGYC 2 cut(s) 60, 68
Hin1II CATG 3 cut(s) 816, 1087, 1274
Hin6I GCGC 2 cut(s) 60, 1335
HinP1I GCGC 2 cut(s) 60, 1335
HincII GTYRAC 3 cut(s) 196, 835, 1633
HindII GTYRAC 3 cut(s) 196, 835, 1633
HindIII AAGCTT 2 cut(s) 305, 1308
HpaII CCGG 3 cut(s) 43, 71, 1666
HphI GGTGA 5 cut(s) 707, 1160, 1272, 1315, 1388
Hpy166II GTNNAC 7 cut(s) 105, 196, 444, 698, 835, 1366, 1633
Hpy8I GTNNAC 7 cut(s) 105, 196, 444, 698, 835, 1366, 1633
Hpy99I CGWCG 3 cut(s) 92, 137, 200
HpyAV CCTTC 6 cut(s) 133, 244, 472, 953, 1345, 1349
HpyCH4III ACNGT 2 cut(s) 1110, 1436
HpyCH4IV ACGT 2 cut(s) 77, 1200
HpyCH4V TGCA 6 cut(s) 451, 472, 620, 650, 907, 947
HpyF10VI GCNNNNNNNGC 6 cut(s) 343, 525, 725, 913, 1172, 1697
HpyF3I CTNAG 8 cut(s) 276, 459, 820, 1035, 1183, 1369, 1428, 1707
HpySE526I ACGT 2 cut(s) 77, 1200
Hsp92I GRCGYC 2 cut(s) 60, 68
Hsp92II CATG 3 cut(s) 816, 1087, 1274
HspAI GCGC 2 cut(s) 60, 1335
KasI GGCGCC 1 cut(s) 59
Kzo9I GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
LmnI GCTCC 5 cut(s) 185, 236, 375, 1171, 1697
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 5 cut(s) 418, 618, 795, 1162, 1678
MaeI CTAG 6 cut(s) 497, 683, 878, 1196, 1319, 1608
MaeII ACGT 2 cut(s) 77, 1200
MaeIII GTNAC 3 cut(s) 64, 73, 1278
MalI GATC 8 cut(s) 131, 176, 501, 560, 928, 1014, 1500, 1595
MboI GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
MboII GAAGA 9 cut(s) 73, 184, 205, 352, 403, 671, 793, 1163, 1578
MflI RGATCY 2 cut(s) 499, 926
MhlI GDGCHC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
MluCI AATT 9 cut(s) 314, 788, 900, 979, 1206, 1226, 1534, 1553, 1568
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 7 cut(s) 8, 275, 317, 851, 1261, 1361, 1682
MmeI TCCRAC 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 1310
MseI TTAA 4 cut(s) 1071, 1244, 1566, 1581
MspA1I CMGCKG 1 cut(s) 1402
MspI CCGG 3 cut(s) 43, 71, 1666
MspR9I CCNGG 4 cut(s) 44, 951, 1326, 1667
Mva1269I GAATGC 1 cut(s) 1521
MvaI CCWGG 2 cut(s) 951, 1326
MvnI CGCG 2 cut(s) 476, 1335
MwoI GCNNNNNNNGC 6 cut(s) 343, 525, 725, 913, 1172, 1697
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 2 cut(s) 44, 1667
NdeI CATATG 2 cut(s) 770, 1285
NdeII GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
NlaIII CATG 3 cut(s) 816, 1087, 1274
NlaIV GGNNCC 6 cut(s) 47, 61, 168, 299, 1062, 1167
NmuCI GTSAC 3 cut(s) 64, 73, 1278
NspV TTCGAA 3 cut(s) 389, 506, 957
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 318
PctI GAATGC 1 cut(s) 1521
PdmI GAANNNNTTC 1 cut(s) 1310
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 99, 203, 417, 1165
PleI GAGTC 7 cut(s) 8, 275, 317, 850, 1261, 1361, 1681
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 7 cut(s) 8, 275, 317, 850, 1261, 1361, 1681
PpuMI RGGWCCY 3 cut(s) 46, 145, 1061
PshAI GACNNNNGTC 2 cut(s) 71, 1271
PshBI ATTAAT 1 cut(s) 1071
PsiI TTATAA 1 cut(s) 1205
Psp124BI GAGCTC 3 cut(s) 190, 241, 1702
Psp5II RGGWCCY 3 cut(s) 46, 145, 1061
Psp6I CCWGG 2 cut(s) 949, 1324
PspFI CCCAGC 1 cut(s) 1402
PspGI CCWGG 2 cut(s) 949, 1324
PspN4I GGNNCC 6 cut(s) 47, 61, 168, 299, 1062, 1167
PspPI GGNCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
PspPPI RGGWCCY 3 cut(s) 46, 145, 1061
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 2 cut(s) 499, 926
PsyI GACNNNGTC 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 1402
RsaI GTAC 3 cut(s) 33, 655, 1137
RsaNI GTAC 3 cut(s) 32, 654, 1136
SacI GAGCTC 3 cut(s) 190, 241, 1702
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 4 cut(s) 1071, 1244, 1566, 1581
SatI GCNGC 4 cut(s) 98, 202, 416, 1164
Sau3AI GATC 8 cut(s) 129, 174, 499, 558, 926, 1012, 1498, 1593
Sau96I GGNCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
SbfI CCTGCAGG 1 cut(s) 652
SchI GAGTC 7 cut(s) 8, 275, 317, 851, 1261, 1361, 1682
ScrFI CCNGG 4 cut(s) 44, 951, 1326, 1667
SdaI CCTGCAGG 1 cut(s) 652
SduI GDGCHC 6 cut(s) 190, 241, 874, 1262, 1333, 1702
SfaNI GCATC 5 cut(s) 418, 618, 795, 1162, 1678
SfcI CTRYAG 1 cut(s) 648
SfoI GGCGCC 1 cut(s) 61
SfuI TTCGAA 3 cut(s) 389, 506, 957
SinI GGWCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse8387I CCTGCAGG 1 cut(s) 652
Sse9I AATT 9 cut(s) 314, 788, 900, 979, 1206, 1226, 1534, 1553, 1568
SsiI CCGC 6 cut(s) 100, 201, 204, 231, 416, 1163
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 6 cut(s) 497, 683, 878, 1196, 1319, 1608
SstI GAGCTC 3 cut(s) 190, 241, 1702
StyD4I CCNGG 4 cut(s) 42, 949, 1324, 1665
StyI CCWWGG 1 cut(s) 1139
TaaI ACNGT 2 cut(s) 1110, 1436
TaiI ACGT 2 cut(s) 80, 1203
TaqI TCGA 9 cut(s) 135, 195, 264, 312, 321, 389, 506, 957, 1042
TaqII GACCGA 1 cut(s) 21
TasI AATT 9 cut(s) 314, 788, 900, 979, 1206, 1226, 1534, 1553, 1568
TatI WGTACW 1 cut(s) 31
TauI GCSGC 3 cut(s) 204, 418, 1166
Tru1I TTAA 4 cut(s) 1071, 1244, 1566, 1581
Tru9I TTAA 4 cut(s) 1071, 1244, 1566, 1581
TscAI CASTG 7 cut(s) 453, 538, 622, 1015, 1125, 1267, 1603
TseFI GTSAC 3 cut(s) 64, 73, 1278
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 3 cut(s) 64, 73, 1278
TspDTI ATGAA 6 cut(s) 307, 420, 741, 1011, 1100, 1303
TspGWI ACGGA 3 cut(s) 105, 122, 939
TspRI CASTG 7 cut(s) 453, 538, 622, 1015, 1125, 1267, 1603
Tth111I GACNNNGTC 1 cut(s) 44
VpaK11BI GGWCC 6 cut(s) 4, 46, 145, 167, 1061, 1106
VspI ATTAAT 1 cut(s) 1071
XagI CCTNNNNNAGG 1 cut(s) 1143
XapI RAATTY 3 cut(s) 314, 788, 1568
XbaI TCTAGA 5 cut(s) 496, 682, 877, 1195, 1318
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 1310
XspI CTAG 6 cut(s) 497, 683, 878, 1196, 1319, 1608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.