MD05G1324800.v1.1

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008029
Physical Location & Seq
Reverse (-)
45136081 .. 45138565
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324800.v1.1.491

Sequence Viewer

Length: 948 bp
ATGAAGAGTGCTTTTTTTATAGTAGTAAATGGACTCCTAGAGAGAGATGATTGGGACGCTGCAATAAAGATTCCTCTTGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCAAAATCTCTTTTGGATTCTGTTGGTGAACCTTGTACACCATCTAATGCTGTCCTCGCCATTATTCGAGGGCATAAGCGTTCACTGGATGTAGCTACCATCTGCCAGGGGAAGATGAAATTCTTCAGTGTATTGATGCTTGCATGGGGTTTAGTGGCAGATGTTGACATTGAGTCTGAGAAATATAGGTGGATGGGAAGTGCTCGACTAGATTTCTATGCTGTTCAACGTATTGTGCATTTGAGGCAATATAATGGATCTATCTCCTTTGTGCCTGCACCTGGCTTTGAAACTTACGGAGAGCCTACTAGTTACAGTAATGCCAGTGAACCTACCAACCAACACAATGCAGGTGCTCCAAATCAAGAGGAGCCTATTAGCATTCGACAGCATGGCTATCCAGGGCCTGATATTAACTTGGCTAGCATGGACTGGAGAACTATTAGCGGACCTTTCGTTTCCATCTGGCTTCACAATGTGCCTTGGGGTGGTGAAGACACAAAGGCAGCTCCTGATGCCAAGTTCTCGGATGGTTATATAGACGTGATAATGATGAGGGTCTGTCCAAAATTATCATTGCTCTCGTTGATGTCTGGGTTGAGCACAGGAAGCCATATCAAATCACCATATGTCCTGTACTTCAAGGTGAAAGCATTTATTTTGCAACCTGGTCCTCTCACCGAGGACCCAACTAAGGAAGGGATAATCGACTCAGATGGCGAGGTCCTGGCTAGAGGCAAAGGAGCATACAAATGTGACCAGAAGACATTGATGGTCTACGATAAACTTCAAGTAACTGTGGATCAAGGTTTAGCTACTCTGTTTTCCCCGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

316

Amino Acids

34.39

Weight (kDa)

5.89

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 8 - 70 4.4e-08 Diacylglycerol kinase catalytic domain
YegS_C PF19279 192 - 290 3.6e-10 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 2 cut(s) 83, 452
AccI GTMKAC 1 cut(s) 888
AciI CCGC 1 cut(s) 558
AclWI GGATC 2 cut(s) 376, 921
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 1 cut(s) 220
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 3 cut(s) 97, 148, 749
AfiI CCNNNNNNNGG 3 cut(s) 392, 599, 805
AgsI TTSAA 4 cut(s) 338, 401, 754, 902
AhdI GACNNNNNGTC 1 cut(s) 283
AhlI ACTAGT 1 cut(s) 419
AjiI CACGTC 1 cut(s) 655
AjnI CCWGG 5 cut(s) 216, 391, 511, 778, 837
AluBI AGCT 3 cut(s) 206, 620, 926
AluI AGCT 3 cut(s) 206, 620, 926
Alw21I GWGCWC 3 cut(s) 316, 469, 716
AlwI GGATC 2 cut(s) 376, 921
AlwNI CAGNNNCTG 3 cut(s) 98, 518, 623
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 2 cut(s) 59, 617
ApoI RAATTY 1 cut(s) 230
Asp700I GAANNNNTTC 1 cut(s) 233
AspS9I GGNCC 5 cut(s) 515, 560, 782, 796, 835
AsuC2I CCSGG 1 cut(s) 941
AsuHPI GGTGA 5 cut(s) 149, 614, 726, 769, 781
AsuNHI GCTAGC 1 cut(s) 533
AvaII GGWCC 4 cut(s) 560, 782, 796, 835
BbsI GAAGAC 2 cut(s) 612, 881
Bbv12I GWGCWC 3 cut(s) 316, 469, 716
BbvI GCAGC 2 cut(s) 46, 629
BccI CCATC 7 cut(s) 160, 218, 298, 581, 635, 821, 877
BciT130I CCWGG 5 cut(s) 218, 393, 513, 780, 839
BcnI CCSGG 1 cut(s) 941
BcuI ACTAGT 1 cut(s) 419
BfaI CTAG 5 cut(s) 38, 320, 420, 534, 843
BfmI CTRYAG 1 cut(s) 90
BfuAI ACCTGC 2 cut(s) 83, 452
BisI GCNGC 2 cut(s) 60, 618
BlsI GCNGC 2 cut(s) 61, 619
Bme1390I CCNGG 6 cut(s) 218, 393, 513, 780, 839, 941
Bme18I GGWCC 4 cut(s) 560, 782, 796, 835
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgBI CACGTC 1 cut(s) 655
BmgT120I GGNCC 5 cut(s) 515, 560, 782, 796, 835
BmiI GGNNCC 2 cut(s) 483, 798
BmrFI CCNGG 6 cut(s) 218, 393, 513, 780, 839, 941
BmsI GCATC 2 cut(s) 237, 616
BmtI GCTAGC 1 cut(s) 537
BpiI GAAGAC 2 cut(s) 612, 881
BpmI CTGGAG 1 cut(s) 565
BpuMI CCSGG 1 cut(s) 941
BsaJI CCNNGG 5 cut(s) 217, 512, 593, 792, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 599, 805
Bse1I ACTGG 4 cut(s) 103, 201, 435, 548
Bse3DI GCAATG 1 cut(s) 686
BseBI CCWGG 5 cut(s) 218, 393, 513, 780, 839
BseDI CCNNGG 5 cut(s) 217, 512, 593, 792, 939
BseGI GGATG 3 cut(s) 205, 309, 646
BseLI CCNNNNNNNGG 3 cut(s) 392, 599, 805
BseMI GCAATG 1 cut(s) 686
BseMII CTCAG 2 cut(s) 279, 837
BseNI ACTGG 4 cut(s) 103, 201, 435, 548
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 46, 629
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 1 cut(s) 517
BsiHKAI GWGCWC 3 cut(s) 316, 469, 716
BsiSI CCGG 1 cut(s) 941
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 392, 599, 805
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 1 cut(s) 517
Bsp1286I GDGCHC 3 cut(s) 316, 469, 716
Bsp1407I TGTACA 1 cut(s) 146
Bsp143I GATC 2 cut(s) 368, 913
BspACI CCGC 1 cut(s) 558
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 2 cut(s) 280, 836
BspLI GGNNCC 2 cut(s) 483, 798
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 2 cut(s) 83, 452
BspOI GCTAGC 1 cut(s) 537
BspPI GGATC 2 cut(s) 376, 921
BsrDI GCAATG 1 cut(s) 686
BsrGI TGTACA 1 cut(s) 146
BsrI ACTGG 4 cut(s) 103, 201, 435, 548
BssECI CCNNGG 5 cut(s) 217, 512, 593, 792, 939
BssMI GATC 2 cut(s) 368, 913
BssT1I CCWWGG 1 cut(s) 593
Bst2UI CCWGG 5 cut(s) 218, 393, 513, 780, 839
Bst4CI ACNGT 2 cut(s) 428, 910
BstAUI TGTACA 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 252, 387, 535
BstDEI CTNAG 3 cut(s) 288, 804, 823
BstF5I GGATG 3 cut(s) 205, 309, 646
BstKTI GATC 2 cut(s) 371, 916
BstMBI GATC 2 cut(s) 368, 913
BstMWI GCNNNNNNNGC 4 cut(s) 167, 355, 626, 720
BstNI CCWGG 5 cut(s) 218, 393, 513, 780, 839
BstSCI CCNGG 6 cut(s) 216, 391, 511, 778, 837, 939
BstSFI CTRYAG 1 cut(s) 90
BstV1I GCAGC 2 cut(s) 46, 629
BstV2I GAAGAC 2 cut(s) 612, 881
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
BsuRI GGCC 1 cut(s) 517
BtrI CACGTC 1 cut(s) 655
BtsCI GGATG 3 cut(s) 205, 309, 646
BtsIMutI CAGTG 3 cut(s) 194, 244, 442
BveI ACCTGC 2 cut(s) 83, 452
Cac8I GCNNGC 3 cut(s) 252, 387, 535
CaiI CAGNNNCTG 3 cut(s) 98, 518, 623
Cfr13I GGNCC 5 cut(s) 515, 560, 782, 796, 835
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 3 cut(s) 96, 147, 748
CviAII CATG 3 cut(s) 255, 503, 538
CviQI GTAC 3 cut(s) 96, 147, 748
DdeI CTNAG 3 cut(s) 288, 804, 823
DpnI GATC 2 cut(s) 370, 915
DpnII GATC 2 cut(s) 368, 913
DraIII CACNNNGTG 1 cut(s) 589
DriI GACNNNNNGTC 1 cut(s) 283
Eam1105I GACNNNNNGTC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 593
Eco47I GGWCC 4 cut(s) 560, 782, 796, 835
Eco57I CTGAAG 1 cut(s) 220
EcoO109I RGGNCCY 3 cut(s) 515, 796, 835
EcoRII CCWGG 5 cut(s) 216, 391, 511, 778, 837
EcoT14I CCWWGG 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 593
FaeI CATG 3 cut(s) 258, 506, 541
FaqI GGGAC 1 cut(s) 68
FatI CATG 3 cut(s) 254, 502, 537
FauNDI CATATG 1 cut(s) 739
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 2 cut(s) 60, 618
FokI GGATG 3 cut(s) 212, 316, 653
Fsp4HI GCNGC 2 cut(s) 60, 618
FspBI CTAG 5 cut(s) 38, 320, 420, 534, 843
GluI GCNGC 2 cut(s) 60, 618
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 1 cut(s) 517
HapII CCGG 1 cut(s) 941
HgaI GACGC 1 cut(s) 65
Hin1II CATG 3 cut(s) 258, 506, 541
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 5 cut(s) 33, 70, 128, 284, 821
HpaII CCGG 1 cut(s) 941
HphI GGTGA 5 cut(s) 149, 614, 726, 769, 781
Hpy166II GTNNAC 6 cut(s) 140, 149, 194, 277, 440, 889
Hpy188I TCNGA 3 cut(s) 289, 640, 826
Hpy188III TCNNGA 2 cut(s) 476, 623
Hpy8I GTNNAC 6 cut(s) 140, 149, 194, 277, 440, 889
HpyAV CCTTC 1 cut(s) 803
HpyCH4III ACNGT 2 cut(s) 428, 910
HpyCH4IV ACGT 2 cut(s) 340, 654
HpyCH4V TGCA 7 cut(s) 62, 92, 254, 349, 389, 461, 775
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 355, 626, 720
HpyF3I CTNAG 3 cut(s) 288, 804, 823
HpySE526I ACGT 2 cut(s) 340, 654
Hsp92II CATG 3 cut(s) 258, 506, 541
Kzo9I GATC 2 cut(s) 368, 913
LmnI GCTCC 4 cut(s) 472, 481, 625, 854
Lsp1109I GCAGC 2 cut(s) 46, 629
LweI GCATC 2 cut(s) 237, 616
MabI ACCWGGT 1 cut(s) 778
MaeI CTAG 5 cut(s) 38, 320, 420, 534, 843
MaeII ACGT 2 cut(s) 340, 654
MaeIII GTNAC 3 cut(s) 422, 866, 904
MalI GATC 2 cut(s) 370, 915
MboI GATC 2 cut(s) 368, 913
MboII GAAGA 5 cut(s) 16, 226, 235, 617, 886
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 3 cut(s) 316, 469, 716
MluCI AATT 2 cut(s) 230, 680
MlyI GAGTC 3 cut(s) 27, 293, 815
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 1 cut(s) 862
MspI CCGG 1 cut(s) 941
MspR9I CCNGG 6 cut(s) 218, 393, 513, 780, 839, 941
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 5 cut(s) 218, 393, 513, 780, 839
MwoI GCNNNNNNNGC 4 cut(s) 167, 355, 626, 720
NciI CCSGG 1 cut(s) 941
NdeI CATATG 1 cut(s) 739
NdeII GATC 2 cut(s) 368, 913
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 3 cut(s) 258, 506, 541
NlaIV GGNNCC 2 cut(s) 483, 798
NmuCI GTSAC 1 cut(s) 866
PaqCI CACCTGC 1 cut(s) 452
PctI GAATGC 1 cut(s) 492
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 70, 128
PkrI GCNGC 2 cut(s) 61, 619
PleI GAGTC 3 cut(s) 27, 292, 815
PpsI GAGTC 3 cut(s) 27, 292, 815
PpuMI RGGWCCY 2 cut(s) 796, 835
Psp5II RGGWCCY 2 cut(s) 796, 835
Psp6I CCWGG 5 cut(s) 216, 391, 511, 778, 837
PspGI CCWGG 5 cut(s) 216, 391, 511, 778, 837
PspN4I GGNNCC 2 cut(s) 483, 798
PspPI GGNCC 5 cut(s) 515, 560, 782, 796, 835
PspPPI RGGWCCY 2 cut(s) 796, 835
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 3 cut(s) 98, 518, 623
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 3 cut(s) 97, 148, 749
RsaNI GTAC 3 cut(s) 96, 147, 748
RseI CAYNNNNRTG 1 cut(s) 862
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 2 cut(s) 60, 618
Sau3AI GATC 2 cut(s) 368, 913
Sau96I GGNCC 5 cut(s) 515, 560, 782, 796, 835
SbfI CCTGCAGG 1 cut(s) 94
SchI GAGTC 3 cut(s) 27, 293, 815
ScrFI CCNGG 6 cut(s) 218, 393, 513, 780, 839, 941
SdaI CCTGCAGG 1 cut(s) 94
SduI GDGCHC 3 cut(s) 316, 469, 716
SexAI ACCWGGT 1 cut(s) 778
SfaNI GCATC 2 cut(s) 237, 616
SfcI CTRYAG 1 cut(s) 90
SinI GGWCC 4 cut(s) 560, 782, 796, 835
SmiMI CAYNNNNRTG 1 cut(s) 862
SpeI ACTAGT 1 cut(s) 419
Sse8387I CCTGCAGG 1 cut(s) 94
Sse9I AATT 2 cut(s) 230, 680
SsiI CCGC 1 cut(s) 558
SspMI CTAG 5 cut(s) 38, 320, 420, 534, 843
StyD4I CCNGG 6 cut(s) 216, 391, 511, 778, 837, 939
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 2 cut(s) 428, 910
TaiI ACGT 2 cut(s) 343, 657
TaqI TCGA 4 cut(s) 178, 316, 496, 819
TasI AATT 2 cut(s) 230, 680
TatI WGTACW 2 cut(s) 146, 747
TfiI GAWTC 2 cut(s) 70, 128
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 3 cut(s) 201, 244, 442
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 2 cut(s) 59, 617
Tsp45I GTSAC 1 cut(s) 866
TspDTI ATGAA 2 cut(s) 17, 242
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 3 cut(s) 201, 244, 442
VpaK11BI GGWCC 4 cut(s) 560, 782, 796, 835
XapI RAATTY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 5 cut(s) 38, 320, 420, 534, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.