Prupe.4G036200_v2.0.a1

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0008029
Physical Location & Seq
Reverse (-)
1712820 .. 1717147
4328 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G036200.2

Sequence Viewer

Length: 1476 bp
ATGGACCGACCCGAGTCCGCTCAACCCATACTCTCCGACCGGGTCCTCATAAACGGCACCGTCACTCCGGTGACTCTATTCGCCGATGGAGTTCTCCGGTGGTCGGAGAGAGGTCAACGAAGCTTGATCGTGGAGAAGGAGGTCCTCGGGTTCGCAGCGGAAGGTCCGAAGATTAGGATCAGAGCCTTAGTGGAAGACGGAGATGGAATCTGCTGCGTCGCGAGCAGAGGAGCTGTGGTGAGGAAAGAATTCGTCTTCAAGCCTCTCTCTGAGGACTCGCATAGGCTCTGGTGCCAGAAGCTCCGCGAACACATTGATTCTCTGGGGCGGCCAAAGAGGCTCTTCGTTTTTGTGAATCCATTTGGTGGGAGCAAATCCGCTTCAAAAATATATGCTGAACACGTAAAACCTCTTTTCCAGGATGCTAATATCCAATTTACACTACAAGAAACTCAATATCAACTACATGCGAAGGAAGTTGCTAAAACACTAGATCTTTCAAAGTATGATGGCATTGTTTGTGTTAGTGGGGATGGAATCTTGGTTGAGGTAGTAAATGGACTCCTTGAGAGAGAGGATTGGGAAGCTGCAATAAAGATGCCTCTTGGAGTAGTTCCTGCAGGTACAGGAAATGGAATGGCAAAATCTCTTTTGGATTCTGTTGGTGAACCTTGTACAGCATCTAATGCTATTCTTGCCATTATTCGAGGGAATAAGCGTTCACTGGATGTAGCTACCATATGGCAGGGGAAGACCAAATTCTTCAGTGTATTGATGCTTGCATGGGGTTTAGTGGCAGATGTTGACATTGAGTCTGAGAAGTATAGGTGGATGGGAAGTGCTCGTCTAGATTTCTATGCTGTTCAACGAATTGTGCATTTGAGGCAATATAATGGATCTATTTCCTTTGTGCCTGCACCTGGCTTTGAAATTTATGGGGAGCCTACTAGTTACAACAACATTAGTGAACCTACTAACCATCACAATAAATGTGGTCCAAGTCAAGAGGAGCCTGTTAACATTCGACAGCATGGCTATGAGGGACCTGATATTAACTTGGAGAACATGGACTGGAGAACTATTAATGGACCATTCGTTTCAGTCTGGCTTCACAATGTCCCTTGGGGAAGTGAAGACACAAAGGCAGCGCCTGATGCCAAGTTCTCAGATGGTTACCTAGATGTGATAATTATGAGAGCTTGCCCAAAACTATCCTTGCTCTCGTTGATGTCAGGGTTGAGCACAGGAAGTCATGTCAATTCACCATATGTCATGTACTTCAAGGTGAAAGCATTAATTTTGGAACCAGGTCCTCTCACTGAGGACCCCACAAAGGGAGGGATTATAGACTCAGATGGTGAGGTCCTGGCTAGGGGCAAAGGAGCATACAAATGTGACCAAAAGACGCTGATGGCCTATGATAAACTCCAAATAACTGTGGATCAAGGTTTAGCTACTCTGTTTTCCCCGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

492

Amino Acids

54.17

Weight (kDa)

5.99

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 56, 291
AccB7I CCANNNNNTGG 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 20
AccII CGCG 2 cut(s) 221, 306
AciI CCGC 5 cut(s) 18, 158, 304, 328, 378
AclWI GGATC 3 cut(s) 185, 904, 1449
AcoI YGGCCR 1 cut(s) 329
AcsI RAATTY 3 cut(s) 248, 758, 930
AcuI CTGAAG 1 cut(s) 748
AfaI GTAC 3 cut(s) 625, 676, 1277
AfiI CCNNNNNNNGG 6 cut(s) 103, 365, 920, 1333, 1334, 1372
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 6 cut(s) 259, 384, 501, 866, 929, 1282
AhdI GACNNNNNGTC 1 cut(s) 811
AhlI ACTAGT 1 cut(s) 947
AjnI CCWGG 4 cut(s) 417, 919, 1306, 1365
AleI CACNNNNGTG 1 cut(s) 68
AloI GAACNNNNNNTCC 2 cut(s) 703, 735
AluBI AGCT 7 cut(s) 123, 233, 301, 587, 734, 1199, 1454
AluI AGCT 7 cut(s) 123, 233, 301, 587, 734, 1199, 1454
Alw21I GWGCWC 2 cut(s) 844, 1244
AlwI GGATC 3 cut(s) 185, 904, 1449
AlwNI CAGNNNCTG 1 cut(s) 1151
Ama87I CYCGRG 2 cut(s) 11, 146
AoxI GGCC 2 cut(s) 329, 1413
ApeKI GCWGC 4 cut(s) 155, 213, 587, 1145
ApoI RAATTY 3 cut(s) 248, 758, 930
AseI ATTAAT 2 cut(s) 1083, 1295
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 1150
AsuC2I CCSGG 2 cut(s) 41, 1469
AsuHPI GGTGA 6 cut(s) 82, 250, 677, 1254, 1297, 1370
AvaI CYCGRG 2 cut(s) 11, 146
BanI GGYRCC 2 cut(s) 56, 291
BbsI GAAGAC 4 cut(s) 201, 247, 758, 1140
Bbv12I GWGCWC 2 cut(s) 844, 1244
BbvI GCAGC 4 cut(s) 167, 200, 574, 1157
BccI CCATC 9 cut(s) 80, 197, 503, 527, 826, 987, 1163, 1349, 1405
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 4 cut(s) 419, 921, 1308, 1367
BcnI CCSGG 2 cut(s) 41, 1469
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 5 cut(s) 491, 848, 948, 1178, 1371
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 1 cut(s) 1151
BfuAI ACCTGC 1 cut(s) 611
BglI GCCNNNNNGGC 1 cut(s) 337
BglII AGATCT 1 cut(s) 493
BisI GCNGC 5 cut(s) 156, 214, 329, 588, 1146
BlsI GCNGC 5 cut(s) 157, 215, 330, 589, 1147
Bme1390I CCNGG 6 cut(s) 41, 419, 921, 1308, 1367, 1469
BmeRI GACNNNNNGTC 1 cut(s) 811
BmeT110I CYCGRG 2 cut(s) 11, 146
BmiI GGNNCC 8 cut(s) 44, 58, 293, 942, 1011, 1044, 1305, 1326
BmrFI CCNGG 6 cut(s) 41, 419, 921, 1308, 1367, 1469
BmsI GCATC 5 cut(s) 412, 588, 689, 765, 1144
BoxI GACNNNNGTC 1 cut(s) 13
BpiI GAAGAC 4 cut(s) 201, 247, 758, 1140
BpmI CTGGAG 1 cut(s) 1093
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 2 cut(s) 41, 1469
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 145, 1121, 1467
BsaWI WCCGGW 2 cut(s) 67, 96
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 222, 252
Bsc4I CCNNNNNNNGG 6 cut(s) 103, 365, 920, 1333, 1334, 1372
Bse1I ACTGG 2 cut(s) 729, 1076
Bse8I GATNNNNATC 1 cut(s) 176
BseBI CCWGG 4 cut(s) 419, 921, 1308, 1367
BseDI CCNNGG 3 cut(s) 145, 1121, 1467
BseGI GGATG 4 cut(s) 427, 538, 733, 837
BseJI GATNNNNATC 1 cut(s) 176
BseLI CCNNNNNNNGG 6 cut(s) 103, 365, 920, 1333, 1334, 1372
BseMII CTCAG 5 cut(s) 261, 807, 1179, 1311, 1365
BseNI ACTGG 2 cut(s) 729, 1076
BseRI GAGGAG 2 cut(s) 243, 1022
BseXI GCAGC 4 cut(s) 167, 200, 574, 1157
BsgI GTGCAG 1 cut(s) 900
Bsh1236I CGCG 2 cut(s) 221, 306
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 2 cut(s) 331, 1415
BshNI GGYRCC 2 cut(s) 56, 291
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 844, 1244
BsiHKCI CYCGRG 2 cut(s) 11, 146
BsiSI CCGG 4 cut(s) 40, 68, 97, 1469
BslFI GGGAC 2 cut(s) 1056, 1103
BslI CCNNNNNNNGG 6 cut(s) 103, 365, 920, 1333, 1334, 1372
BsmFI GGGAC 2 cut(s) 1056, 1103
BsnI GGCC 2 cut(s) 331, 1415
BsoBI CYCGRG 2 cut(s) 11, 146
Bsp1286I GDGCHC 2 cut(s) 844, 1244
Bsp1407I TGTACA 1 cut(s) 674
Bsp143I GATC 5 cut(s) 126, 177, 493, 896, 1441
Bsp68I TCGCGA 1 cut(s) 221
BspACI CCGC 5 cut(s) 18, 158, 304, 328, 378
BspANI GGCC 2 cut(s) 331, 1415
BspCNI CTCAG 5 cut(s) 262, 808, 1178, 1312, 1364
BspFNI CGCG 2 cut(s) 221, 306
BspLI GGNNCC 8 cut(s) 44, 58, 293, 942, 1011, 1044, 1305, 1326
BspMAI CTGCAG 1 cut(s) 622
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 185, 904, 1449
BspQI GCTCTTC 1 cut(s) 347
BspT107I GGYRCC 2 cut(s) 56, 291
BsrBI CCGCTC 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 674
BsrI ACTGG 2 cut(s) 729, 1076
BssECI CCNNGG 3 cut(s) 145, 1121, 1467
BssMI GATC 5 cut(s) 126, 177, 493, 896, 1441
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 4 cut(s) 419, 921, 1308, 1367
Bst4CI ACNGT 2 cut(s) 61, 1438
Bst6I CTCTTC 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 686
BstAUI TGTACA 1 cut(s) 674
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 4 cut(s) 223, 780, 915, 1201
BstDEI CTNAG 6 cut(s) 187, 270, 816, 1165, 1320, 1351
BstEII GGTNACC 1 cut(s) 1172
BstF5I GGATG 4 cut(s) 427, 538, 733, 837
BstFNI CGCG 2 cut(s) 221, 306
BstH2I RGCGCY 1 cut(s) 1151
BstHHI GCGC 1 cut(s) 1150
BstKTI GATC 5 cut(s) 129, 180, 496, 899, 1444
BstMBI GATC 5 cut(s) 126, 177, 493, 896, 1441
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 6 cut(s) 222, 337, 686, 695, 883, 1154
BstNI CCWGG 4 cut(s) 419, 921, 1308, 1367
BstNSI RCATGY 1 cut(s) 470
BstPAI GACNNNNGTC 1 cut(s) 13
BstPI GGTNACC 1 cut(s) 1172
BstSCI CCNGG 6 cut(s) 39, 417, 919, 1306, 1365, 1467
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 2 cut(s) 221, 306
BstV1I GCAGC 4 cut(s) 167, 200, 574, 1157
BstV2I GAAGAC 4 cut(s) 201, 247, 758, 1140
BstX2I RGATCY 2 cut(s) 493, 896
BstYI RGATCY 2 cut(s) 493, 896
BsuRI GGCC 2 cut(s) 331, 1415
BtsCI GGATG 4 cut(s) 427, 538, 733, 837
BtsIMutI CAGTG 3 cut(s) 722, 772, 1317
BtuMI TCGCGA 1 cut(s) 221
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 4 cut(s) 223, 780, 915, 1201
CaiI CAGNNNCTG 1 cut(s) 1151
CfoI GCGC 1 cut(s) 1150
CseI GACGC 2 cut(s) 205, 1414
CsiI ACCWGGT 1 cut(s) 1306
Csp6I GTAC 3 cut(s) 624, 675, 1276
CspCI CAANNNNNGTGG 2 cut(s) 973, 1008
CviAII CATG 6 cut(s) 467, 783, 1031, 1066, 1253, 1273
CviQI GTAC 3 cut(s) 624, 675, 1276
DdeI CTNAG 6 cut(s) 187, 270, 816, 1165, 1320, 1351
DpnI GATC 5 cut(s) 128, 179, 495, 898, 1443
DpnII GATC 5 cut(s) 126, 177, 493, 896, 1441
DriI GACNNNNNGTC 1 cut(s) 811
EaeI YGGCCR 1 cut(s) 329
Eam1104I CTCTTC 1 cut(s) 347
Eam1105I GACNNNNNGTC 1 cut(s) 811
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 1121
Eco57I CTGAAG 1 cut(s) 748
Eco88I CYCGRG 2 cut(s) 11, 146
Eco91I GGTNACC 1 cut(s) 1172
EcoO109I RGGNCCY 6 cut(s) 43, 142, 1043, 1310, 1324, 1363
EcoO65I GGTNACC 1 cut(s) 1172
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 4 cut(s) 417, 919, 1306, 1365
EcoT14I CCWWGG 1 cut(s) 1121
ErhI CCWWGG 1 cut(s) 1121
FaeI CATG 6 cut(s) 470, 786, 1034, 1069, 1256, 1276
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 1056, 1103
FatI CATG 6 cut(s) 466, 782, 1030, 1065, 1252, 1272
FauNDI CATATG 2 cut(s) 740, 1267
Fnu4HI GCNGC 5 cut(s) 156, 214, 329, 588, 1146
FokI GGATG 4 cut(s) 434, 545, 740, 844
Fsp4HI GCNGC 5 cut(s) 156, 214, 329, 588, 1146
FspBI CTAG 5 cut(s) 491, 848, 948, 1178, 1371
GlaI GCGC 1 cut(s) 1149
GluI GCNGC 5 cut(s) 156, 214, 329, 588, 1146
GsuI CTGGAG 1 cut(s) 1093
HaeII RGCGCY 1 cut(s) 1151
HaeIII GGCC 2 cut(s) 331, 1415
HapII CCGG 4 cut(s) 40, 68, 97, 1469
HgaI GACGC 2 cut(s) 205, 1414
HhaI GCGC 1 cut(s) 1150
Hin1II CATG 6 cut(s) 470, 786, 1034, 1069, 1256, 1276
Hin6I GCGC 1 cut(s) 1148
HinP1I GCGC 1 cut(s) 1148
HincII GTYRAC 3 cut(s) 116, 805, 1018
HindII GTYRAC 3 cut(s) 116, 805, 1018
HindIII AAGCTT 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 1018
HpaII CCGG 4 cut(s) 40, 68, 97, 1469
HphI GGTGA 6 cut(s) 82, 250, 677, 1254, 1297, 1370
Hpy166II GTNNAC 6 cut(s) 116, 668, 722, 805, 968, 1018
Hpy188I TCNGA 8 cut(s) 37, 106, 168, 182, 271, 817, 1168, 1354
Hpy188III TCNNGA 3 cut(s) 220, 848, 1004
Hpy8I GTNNAC 6 cut(s) 116, 668, 722, 805, 968, 1018
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 130, 155, 466
HpyCH4III ACNGT 2 cut(s) 61, 1438
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 590, 620, 782, 877, 917
HpyF10VI GCNNNNNNNGC 6 cut(s) 222, 337, 686, 695, 883, 1154
HpyF3I CTNAG 6 cut(s) 187, 270, 816, 1165, 1320, 1351
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 6 cut(s) 470, 786, 1034, 1069, 1256, 1276
HspAI GCGC 1 cut(s) 1148
KspAI GTTAAC 1 cut(s) 1018
Kzo9I GATC 5 cut(s) 126, 177, 493, 896, 1441
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 6 cut(s) 230, 306, 369, 940, 1009, 1382
Lsp1109I GCAGC 4 cut(s) 167, 200, 574, 1157
LweI GCATC 5 cut(s) 412, 588, 689, 765, 1144
MabI ACCWGGT 1 cut(s) 1306
MaeI CTAG 5 cut(s) 491, 848, 948, 1178, 1371
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 5 cut(s) 61, 70, 950, 1172, 1394
MalI GATC 5 cut(s) 128, 179, 495, 898, 1443
MbiI CCGCTC 1 cut(s) 20
MboI GATC 5 cut(s) 126, 177, 493, 896, 1441
MboII GAAGA 7 cut(s) 181, 206, 247, 334, 754, 763, 1145
MflI RGATCY 2 cut(s) 493, 896
MhlI GDGCHC 2 cut(s) 844, 1244
MluCI AATT 8 cut(s) 248, 434, 758, 870, 930, 1188, 1258, 1296
MlyI GAGTC 6 cut(s) 23, 67, 269, 555, 821, 1343
MmeI TCCRAC 2 cut(s) 60, 84
MroXI GAANNNNTTC 1 cut(s) 248
MseI TTAA 4 cut(s) 1017, 1053, 1083, 1295
MslI CAYNNNNRTG 3 cut(s) 68, 1035, 1390
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 4 cut(s) 40, 68, 97, 1469
MspR9I CCNGG 6 cut(s) 41, 419, 921, 1308, 1367, 1469
MvaI CCWGG 4 cut(s) 419, 921, 1308, 1367
MvnI CGCG 2 cut(s) 221, 306
MwoI GCNNNNNNNGC 6 cut(s) 222, 337, 686, 695, 883, 1154
NciI CCSGG 2 cut(s) 41, 1469
NdeI CATATG 2 cut(s) 740, 1267
NdeII GATC 5 cut(s) 126, 177, 493, 896, 1441
NlaIII CATG 6 cut(s) 470, 786, 1034, 1069, 1256, 1276
NlaIV GGNNCC 8 cut(s) 44, 58, 293, 942, 1011, 1044, 1305, 1326
NmuCI GTSAC 3 cut(s) 61, 70, 1394
NruI TCGCGA 1 cut(s) 221
NspI RCATGY 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 68
PciSI GCTCTTC 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 5 cut(s) 207, 317, 355, 537, 656
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 5 cut(s) 157, 215, 330, 589, 1147
PleI GAGTC 6 cut(s) 22, 67, 269, 555, 820, 1343
PpsI GAGTC 6 cut(s) 22, 67, 269, 555, 820, 1343
Ppu21I YACGTR 1 cut(s) 403
PpuMI RGGWCCY 6 cut(s) 43, 142, 1043, 1310, 1324, 1363
PshAI GACNNNNGTC 1 cut(s) 13
PshBI ATTAAT 2 cut(s) 1083, 1295
Psp5II RGGWCCY 6 cut(s) 43, 142, 1043, 1310, 1324, 1363
Psp6I CCWGG 4 cut(s) 417, 919, 1306, 1365
PspEI GGTNACC 1 cut(s) 1172
PspGI CCWGG 4 cut(s) 417, 919, 1306, 1365
PspN4I GGNNCC 8 cut(s) 44, 58, 293, 942, 1011, 1044, 1305, 1326
PspPPI RGGWCCY 6 cut(s) 43, 142, 1043, 1310, 1324, 1363
PstI CTGCAG 1 cut(s) 622
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 2 cut(s) 493, 896
PsyI GACNNNGTC 1 cut(s) 41
RruI TCGCGA 1 cut(s) 221
RsaI GTAC 3 cut(s) 625, 676, 1277
RsaNI GTAC 3 cut(s) 624, 675, 1276
RseI CAYNNNNRTG 3 cut(s) 68, 1035, 1390
SapI GCTCTTC 1 cut(s) 347
SaqAI TTAA 4 cut(s) 1017, 1053, 1083, 1295
SatI GCNGC 5 cut(s) 156, 214, 329, 588, 1146
Sau3AI GATC 5 cut(s) 126, 177, 493, 896, 1441
SbfI CCTGCAGG 1 cut(s) 622
SchI GAGTC 6 cut(s) 23, 67, 269, 555, 821, 1343
ScrFI CCNGG 6 cut(s) 41, 419, 921, 1308, 1367, 1469
SdaI CCTGCAGG 1 cut(s) 622
SduI GDGCHC 2 cut(s) 844, 1244
SexAI ACCWGGT 1 cut(s) 1306
SfaNI GCATC 5 cut(s) 412, 588, 689, 765, 1144
SfcI CTRYAG 1 cut(s) 618
SmiMI CAYNNNNRTG 3 cut(s) 68, 1035, 1390
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SpeI ACTAGT 1 cut(s) 947
Sse8387I CCTGCAGG 1 cut(s) 622
Sse9I AATT 8 cut(s) 248, 434, 758, 870, 930, 1188, 1258, 1296
SsiI CCGC 5 cut(s) 18, 158, 304, 328, 378
SspMI CTAG 5 cut(s) 491, 848, 948, 1178, 1371
StyD4I CCNGG 6 cut(s) 39, 417, 919, 1306, 1365, 1467
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 2 cut(s) 61, 1438
TaiI ACGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 706, 1024
TaqII GACCGA 1 cut(s) 21
TasI AATT 8 cut(s) 248, 434, 758, 870, 930, 1188, 1258, 1296
TatI WGTACW 2 cut(s) 674, 1275
TauI GCSGC 1 cut(s) 331
TfiI GAWTC 5 cut(s) 207, 317, 355, 537, 656
Tru1I TTAA 4 cut(s) 1017, 1053, 1083, 1295
Tru9I TTAA 4 cut(s) 1017, 1053, 1083, 1295
TscAI CASTG 3 cut(s) 729, 772, 1324
TseFI GTSAC 3 cut(s) 61, 70, 1394
TseI GCWGC 4 cut(s) 155, 213, 587, 1145
Tsp45I GTSAC 3 cut(s) 61, 70, 1394
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 3 cut(s) 729, 772, 1324
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 365
VspI ATTAAT 2 cut(s) 1083, 1295
XapI RAATTY 3 cut(s) 248, 758, 930
XbaI TCTAGA 1 cut(s) 847
XceI RCATGY 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 5 cut(s) 491, 848, 948, 1178, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.