pycom05g29900

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008029
Physical Location & Seq
Reverse (-)
30226971 .. 30230219
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29900.10

Sequence Viewer

Length: 1488 bp
ATGGACCGACCCGGACCCGAGTCTGCTCCCCCGCAACCCCTACTCTCCGACCGGGTCCTCATAAGCGGCGTCGTCACACCCGTGGCTCTATTCTCCGACGGAGTGCTCCGGTGGTCGGAGAGAAGTCAACGGAGCTTGATCTTCGAGAAGGAGGTCCTGGGGTTCGCGGCGGAAGGTCCGAAGATTAGGATAAGAGCTTTTGTGGAAGGCGGAGCTGGAATCTGCTGTGGCGCGAACAGAGGAGCTGTGGCGAGGAAGGACTTGGTGTTCGAGCCTCTCTCTGAGGACTCGCACAGGCTCTGGTGCCAGAAGCTCGGGGATTACATCGATTCTCTCGGACGGCCAAAGAGGCTGTTTGTTTTTGTGAATCCGTTTGGTGGTAGCAAATCTGCTTCAAGGATTTATGTTGATCACGTAAAACCTCTTTTCGAGGATGCTGATATCCAATTTACGCTGCAAGAAACTCAATATCAACTACATGCGAAGGAAGTTGTGAAAACACTAGATCTTTCAAAGTATGATGGCATTGTTTGTGTTAGTGGGGACGGAATTTTGGTTGAGGTAGTAAATGGACTCCTAGAGAGAGAGGATTGGGACGCTGCAATAAAGATTCCTCTTGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCAAAATCTCTTTTGGATTCTGTTGGTGAACCTTGTACACCGTCTAATGCTGTCCTCGCCATTATTCGAGGGCATAAGCGTTCACTGGATGTAGCTACCATCTGCCAGGGGAAGATGAAATTCTTCAGTGTATTGATGCTTGCATGGGGTTTAGTGGCAGATGTTGACATTGAGTCTGAGAAATATAGGTGGATGGGAAGTGCTCGACTAGATTTCTATGCTGTTCAACGGATTGTGCATTTGAGGCAATATAATGGATCTATCTCCTTTGTGCCTGCACCTGGCTTTGAAGCTTACGGAGAGCCTACTAGTTACAGTAATGCCAGTGAACCTACTAACCAACACAATGCAGGTGCTCCAAATCAAGAGGAGCTGATTAGCATTCGACAACATGGCTATCCAGGGCCTGATATTAACTTGGCTAGCATGGACTGGAGAACTATTAGCGGACCGTTTGTTTCCATCTGGCTTCACAATGTGCCTTGGGGTGGTGAAGACACAAAGGCAGCTCCCGATGCCAAGTTCTCGGATGGTTATATAGACGTGATAATGATGAGGGCCTGTCCAAAACTATCATTGCTTTCATTGATGTCTGGGTTGAGCACAGGAAGCCATATCAAATCACCATATGTCTTGTACTTCAAGGTGAAAGCATTTATTTTGCAACCTGGTCCTCTCACCAAGGACCCAACTCAGGAGGGGATAATCGACTCAGATGGCGAGGTCCTGGCTAGAGGCAAAGGAGCATACAAATGTGACCAGAAGACATTGATGGTCTACGATAAACTTCAAGTAACTGTGGATCAAGGTTTAGCTACTCTGTTTTCCCCCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

496

Amino Acids

54.14

Weight (kDa)

5.92

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 992
Acc36I ACCTGC 2 cut(s) 623, 992
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 1428
AccII CGCG 2 cut(s) 167, 233
AciI CCGC 6 cut(s) 32, 66, 167, 170, 210, 1098
AclWI GGATC 2 cut(s) 916, 1461
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 2 cut(s) 549, 770
AcuI CTGAAG 1 cut(s) 760
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 1129
AfaI GTAC 3 cut(s) 637, 688, 1289
AfiI CCNNNNNNNGG 5 cut(s) 115, 377, 932, 1139, 1345
AgsI TTSAA 6 cut(s) 396, 513, 878, 941, 1294, 1442
AhdI GACNNNNNGTC 1 cut(s) 823
AhlI ACTAGT 1 cut(s) 959
AjiI CACGTC 1 cut(s) 1195
AjnI CCWGG 6 cut(s) 156, 756, 931, 1051, 1318, 1377
AleI CACNNNNGTG 1 cut(s) 80
AloI GAACNNNNNNTCC 2 cut(s) 251, 283
Alw21I GWGCWC 4 cut(s) 108, 856, 1009, 1256
AlwI GGATC 2 cut(s) 916, 1461
AlwNI CAGNNNCTG 3 cut(s) 300, 638, 1058
Ama87I CYCGRG 2 cut(s) 17, 314
AoxI GGCC 3 cut(s) 341, 1055, 1209
ApeKI GCWGC 3 cut(s) 454, 599, 1157
ApoI RAATTY 2 cut(s) 549, 770
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
Asp700I GAANNNNTTC 1 cut(s) 773
AspLEI GCGC 1 cut(s) 233
AsuC2I CCSGG 2 cut(s) 12, 53
AsuHPI GGTGA 5 cut(s) 689, 1154, 1266, 1309, 1321
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 17, 314
AvaII GGWCC 9 cut(s) 4, 14, 55, 154, 176, 1100, 1322, 1336, 1375
BanI GGYRCC 1 cut(s) 303
BbsI GAAGAC 2 cut(s) 1152, 1421
Bbv12I GWGCWC 4 cut(s) 108, 856, 1009, 1256
BbvI GCAGC 3 cut(s) 441, 586, 1169
BccI CCATC 7 cut(s) 515, 758, 838, 1121, 1175, 1361, 1417
BceAI ACGGC 1 cut(s) 356
BciT130I CCWGG 6 cut(s) 158, 758, 933, 1053, 1320, 1379
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 2 cut(s) 12, 53
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 6 cut(s) 503, 578, 860, 960, 1074, 1383
BfmI CTRYAG 1 cut(s) 630
BfuAI ACCTGC 2 cut(s) 623, 992
BglI GCCNNNNNGGC 1 cut(s) 349
BglII AGATCT 1 cut(s) 505
BisI GCNGC 5 cut(s) 67, 168, 455, 600, 1158
BlsI GCNGC 5 cut(s) 68, 169, 456, 601, 1159
Bme1390I CCNGG 8 cut(s) 12, 53, 158, 758, 933, 1053, 1320, 1379
Bme18I GGWCC 9 cut(s) 4, 14, 55, 154, 176, 1100, 1322, 1336, 1375
BmeRI GACNNNNNGTC 1 cut(s) 823
BmeT110I CYCGRG 2 cut(s) 17, 314
BmgBI CACGTC 1 cut(s) 1195
BmiI GGNNCC 4 cut(s) 16, 56, 305, 1338
BmrFI CCNGG 8 cut(s) 12, 53, 158, 758, 933, 1053, 1320, 1379
BmsI GCATC 3 cut(s) 424, 777, 1156
BmtI GCTAGC 1 cut(s) 1077
BoxI GACNNNNGTC 1 cut(s) 19
BpiI GAAGAC 2 cut(s) 1152, 1421
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 1 cut(s) 1105
BpuMI CCSGG 2 cut(s) 12, 53
Bsa29I ATCGAT 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 6 cut(s) 81, 157, 757, 1052, 1133, 1332
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 5 cut(s) 115, 377, 932, 1139, 1345
Bse1I ACTGG 4 cut(s) 643, 741, 975, 1088
Bse3DI GCAATG 1 cut(s) 1226
BseBI CCWGG 6 cut(s) 158, 758, 933, 1053, 1320, 1379
BseCI ATCGAT 1 cut(s) 327
BseDI CCNNGG 6 cut(s) 81, 157, 757, 1052, 1133, 1332
BseGI GGATG 4 cut(s) 439, 745, 849, 1186
BseLI CCNNNNNNNGG 5 cut(s) 115, 377, 932, 1139, 1345
BseMI GCAATG 1 cut(s) 1226
BseMII CTCAG 4 cut(s) 273, 819, 1358, 1377
BseNI ACTGG 4 cut(s) 643, 741, 975, 1088
BseRI GAGGAG 2 cut(s) 255, 1034
BseXI GCAGC 3 cut(s) 441, 586, 1169
BsgI GTGCAG 1 cut(s) 912
Bsh1236I CGCG 2 cut(s) 167, 233
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 3 cut(s) 343, 1057, 1211
BshNI GGYRCC 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 4 cut(s) 108, 856, 1009, 1256
BsiHKCI CYCGRG 2 cut(s) 17, 314
BsiSI CCGG 3 cut(s) 12, 52, 109
BslFI GGGAC 2 cut(s) 557, 608
BslI CCNNNNNNNGG 5 cut(s) 115, 377, 932, 1139, 1345
BsmFI GGGAC 2 cut(s) 557, 608
BsmI GAATGC 1 cut(s) 1032
BsnI GGCC 3 cut(s) 343, 1057, 1211
BsoBI CYCGRG 2 cut(s) 17, 314
Bsp1286I GDGCHC 4 cut(s) 108, 856, 1009, 1256
Bsp1407I TGTACA 1 cut(s) 686
Bsp143I GATC 5 cut(s) 138, 409, 505, 908, 1453
BspACI CCGC 6 cut(s) 32, 66, 167, 170, 210, 1098
BspANI GGCC 3 cut(s) 343, 1057, 1211
BspCNI CTCAG 4 cut(s) 274, 820, 1357, 1376
BspDI ATCGAT 1 cut(s) 327
BspFNI CGCG 2 cut(s) 167, 233
BspLI GGNNCC 4 cut(s) 16, 56, 305, 1338
BspMAI CTGCAG 1 cut(s) 634
BspMI ACCTGC 2 cut(s) 623, 992
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 2 cut(s) 916, 1461
BspT107I GGYRCC 1 cut(s) 303
BsrDI GCAATG 1 cut(s) 1226
BsrGI TGTACA 1 cut(s) 686
BsrI ACTGG 4 cut(s) 643, 741, 975, 1088
BssECI CCNNGG 6 cut(s) 81, 157, 757, 1052, 1133, 1332
BssMI GATC 5 cut(s) 138, 409, 505, 908, 1453
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 1133, 1332
Bst2UI CCWGG 6 cut(s) 158, 758, 933, 1053, 1320, 1379
Bst4CI ACNGT 4 cut(s) 693, 968, 1104, 1450
BstACI GRCGYC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 686
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 3 cut(s) 792, 927, 1075
BstDEI CTNAG 4 cut(s) 282, 828, 1344, 1363
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 4 cut(s) 439, 745, 849, 1186
BstFNI CGCG 2 cut(s) 167, 233
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 5 cut(s) 141, 412, 508, 911, 1456
BstMBI GATC 5 cut(s) 138, 409, 505, 908, 1453
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 5 cut(s) 349, 707, 895, 1166, 1260
BstNI CCWGG 6 cut(s) 158, 758, 933, 1053, 1320, 1379
BstNSI RCATGY 1 cut(s) 482
BstPAI GACNNNNGTC 1 cut(s) 19
BstSCI CCNGG 8 cut(s) 10, 51, 156, 756, 931, 1051, 1318, 1377
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 167, 233
BstV1I GCAGC 3 cut(s) 441, 586, 1169
BstV2I GAAGAC 2 cut(s) 1152, 1421
BstX2I RGATCY 2 cut(s) 505, 908
BstYI RGATCY 2 cut(s) 505, 908
Bsu15I ATCGAT 1 cut(s) 327
BsuRI GGCC 3 cut(s) 343, 1057, 1211
BsuTUI ATCGAT 1 cut(s) 327
BtgI CCRYGG 1 cut(s) 81
BtrI CACGTC 1 cut(s) 1195
BtsCI GGATG 4 cut(s) 439, 745, 849, 1186
BtsIMutI CAGTG 3 cut(s) 734, 784, 982
BveI ACCTGC 2 cut(s) 623, 992
Cac8I GCNNGC 3 cut(s) 792, 927, 1075
CaiI CAGNNNCTG 3 cut(s) 300, 638, 1058
CfoI GCGC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 327
CpoI CGGWCCG 1 cut(s) 1100
CseI GACGC 2 cut(s) 58, 605
CsiI ACCWGGT 1 cut(s) 1318
Csp6I GTAC 3 cut(s) 636, 687, 1288
CspI CGGWCCG 1 cut(s) 1100
CviAII CATG 4 cut(s) 479, 795, 1043, 1078
CviQI GTAC 3 cut(s) 636, 687, 1288
DdeI CTNAG 4 cut(s) 282, 828, 1344, 1363
DpnI GATC 5 cut(s) 140, 411, 507, 910, 1455
DpnII GATC 5 cut(s) 138, 409, 505, 908, 1453
DraIII CACNNNGTG 1 cut(s) 1129
DriI GACNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 341
Eam1105I GACNNNNNGTC 1 cut(s) 823
EciI GGCGGA 2 cut(s) 185, 225
Eco130I CCWWGG 2 cut(s) 1133, 1332
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 9 cut(s) 4, 14, 55, 154, 176, 1100, 1322, 1336, 1375
Eco57I CTGAAG 1 cut(s) 760
Eco88I CYCGRG 2 cut(s) 17, 314
EcoO109I RGGNCCY 6 cut(s) 55, 154, 1055, 1209, 1336, 1375
EcoRII CCWGG 6 cut(s) 156, 756, 931, 1051, 1318, 1377
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 1133, 1332
ErhI CCWWGG 2 cut(s) 1133, 1332
FaeI CATG 4 cut(s) 482, 798, 1046, 1081
FaqI GGGAC 2 cut(s) 557, 608
FatI CATG 4 cut(s) 478, 794, 1042, 1077
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 1279
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 1 cut(s) 1428
Fnu4HI GCNGC 5 cut(s) 67, 168, 455, 600, 1158
FokI GGATG 4 cut(s) 446, 752, 856, 1193
Fsp4HI GCNGC 5 cut(s) 67, 168, 455, 600, 1158
FspBI CTAG 6 cut(s) 503, 578, 860, 960, 1074, 1383
GlaI GCGC 1 cut(s) 232
GluI GCNGC 5 cut(s) 67, 168, 455, 600, 1158
GsuI CTGGAG 1 cut(s) 1105
HaeIII GGCC 3 cut(s) 343, 1057, 1211
HapII CCGG 3 cut(s) 12, 52, 109
HgaI GACGC 2 cut(s) 58, 605
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 4 cut(s) 482, 798, 1046, 1081
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 2 cut(s) 128, 817
HindII GTYRAC 2 cut(s) 128, 817
HindIII AAGCTT 1 cut(s) 942
HpaII CCGG 3 cut(s) 12, 52, 109
HphI GGTGA 5 cut(s) 689, 1154, 1266, 1309, 1321
Hpy166II GTNNAC 7 cut(s) 128, 680, 689, 734, 817, 980, 1429
Hpy188I TCNGA 9 cut(s) 49, 97, 118, 180, 283, 338, 829, 1180, 1366
Hpy188III TCNNGA 4 cut(s) 145, 1016, 1163, 1346
Hpy8I GTNNAC 7 cut(s) 128, 680, 689, 734, 817, 980, 1429
Hpy99I CGWCG 2 cut(s) 74, 101
HpyAV CCTTC 5 cut(s) 142, 167, 200, 250, 478
HpyCH4III ACNGT 4 cut(s) 693, 968, 1104, 1450
HpyCH4IV ACGT 2 cut(s) 414, 1194
HpyCH4V TGCA 8 cut(s) 457, 602, 632, 794, 889, 929, 1001, 1315
HpyF10VI GCNNNNNNNGC 5 cut(s) 349, 707, 895, 1166, 1260
HpyF3I CTNAG 4 cut(s) 282, 828, 1344, 1363
HpySE526I ACGT 2 cut(s) 414, 1194
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 4 cut(s) 482, 798, 1046, 1081
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 138, 409, 505, 908, 1453
LmnI GCTCC 9 cut(s) 31, 111, 132, 212, 242, 1012, 1021, 1165, 1394
Lsp1109I GCAGC 3 cut(s) 441, 586, 1169
LweI GCATC 3 cut(s) 424, 777, 1156
MabI ACCWGGT 1 cut(s) 1318
MaeI CTAG 6 cut(s) 503, 578, 860, 960, 1074, 1383
MaeII ACGT 2 cut(s) 414, 1194
MaeIII GTNAC 4 cut(s) 73, 962, 1406, 1444
MalI GATC 5 cut(s) 140, 411, 507, 910, 1455
MboI GATC 5 cut(s) 138, 409, 505, 908, 1453
MboII GAAGA 6 cut(s) 133, 193, 766, 775, 1157, 1426
MflI RGATCY 2 cut(s) 505, 908
MhlI GDGCHC 4 cut(s) 108, 856, 1009, 1256
MluCI AATT 3 cut(s) 446, 549, 770
MlyI GAGTC 5 cut(s) 29, 281, 567, 833, 1355
MmeI TCCRAC 3 cut(s) 72, 96, 120
MroXI GAANNNNTTC 1 cut(s) 773
MseI TTAA 1 cut(s) 1065
MslI CAYNNNNRTG 2 cut(s) 80, 1402
MspI CCGG 3 cut(s) 12, 52, 109
MspR9I CCNGG 8 cut(s) 12, 53, 158, 758, 933, 1053, 1320, 1379
Mva1269I GAATGC 1 cut(s) 1032
MvaI CCWGG 6 cut(s) 158, 758, 933, 1053, 1320, 1379
MvnI CGCG 2 cut(s) 167, 233
MwoI GCNNNNNNNGC 5 cut(s) 349, 707, 895, 1166, 1260
NciI CCSGG 2 cut(s) 12, 53
NdeI CATATG 1 cut(s) 1279
NdeII GATC 5 cut(s) 138, 409, 505, 908, 1453
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 4 cut(s) 482, 798, 1046, 1081
NlaIV GGNNCC 4 cut(s) 16, 56, 305, 1338
NmuCI GTSAC 2 cut(s) 73, 1406
NspI RCATGY 1 cut(s) 482
OliI CACNNNNGTG 1 cut(s) 80
PaqCI CACCTGC 1 cut(s) 992
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PctI GAATGC 1 cut(s) 1032
PdmI GAANNNNTTC 1 cut(s) 773
PfeI GAWTC 5 cut(s) 219, 329, 367, 610, 668
PflFI GACNNNGTC 1 cut(s) 53
PkrI GCNGC 5 cut(s) 68, 169, 456, 601, 1159
PleI GAGTC 5 cut(s) 28, 281, 567, 832, 1355
PpsI GAGTC 5 cut(s) 28, 281, 567, 832, 1355
Ppu21I YACGTR 1 cut(s) 415
PpuMI RGGWCCY 4 cut(s) 55, 154, 1336, 1375
PshAI GACNNNNGTC 1 cut(s) 19
Psp5II RGGWCCY 4 cut(s) 55, 154, 1336, 1375
Psp6I CCWGG 6 cut(s) 156, 756, 931, 1051, 1318, 1377
PspGI CCWGG 6 cut(s) 156, 756, 931, 1051, 1318, 1377
PspN4I GGNNCC 4 cut(s) 16, 56, 305, 1338
PspPPI RGGWCCY 4 cut(s) 55, 154, 1336, 1375
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 3 cut(s) 300, 638, 1058
PsuI RGATCY 2 cut(s) 505, 908
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 3 cut(s) 637, 688, 1289
RsaNI GTAC 3 cut(s) 636, 687, 1288
RseI CAYNNNNRTG 2 cut(s) 80, 1402
Rsr2I CGGWCCG 1 cut(s) 1100
RsrII CGGWCCG 1 cut(s) 1100
SaqAI TTAA 1 cut(s) 1065
SatI GCNGC 5 cut(s) 67, 168, 455, 600, 1158
Sau3AI GATC 5 cut(s) 138, 409, 505, 908, 1453
SbfI CCTGCAGG 1 cut(s) 634
SchI GAGTC 5 cut(s) 29, 281, 567, 833, 1355
ScrFI CCNGG 8 cut(s) 12, 53, 158, 758, 933, 1053, 1320, 1379
SdaI CCTGCAGG 1 cut(s) 634
SduI GDGCHC 4 cut(s) 108, 856, 1009, 1256
SexAI ACCWGGT 1 cut(s) 1318
SfaNI GCATC 3 cut(s) 424, 777, 1156
SfcI CTRYAG 1 cut(s) 630
SinI GGWCC 9 cut(s) 4, 14, 55, 154, 176, 1100, 1322, 1336, 1375
SmiMI CAYNNNNRTG 2 cut(s) 80, 1402
SpeI ACTAGT 1 cut(s) 959
Sse8387I CCTGCAGG 1 cut(s) 634
Sse9I AATT 3 cut(s) 446, 549, 770
SsiI CCGC 6 cut(s) 32, 66, 167, 170, 210, 1098
SspMI CTAG 6 cut(s) 503, 578, 860, 960, 1074, 1383
StyD4I CCNGG 8 cut(s) 10, 51, 156, 756, 931, 1051, 1318, 1377
StyI CCWWGG 2 cut(s) 1133, 1332
TaaI ACNGT 4 cut(s) 693, 968, 1104, 1450
TaiI ACGT 2 cut(s) 417, 1197
TaqI TCGA 8 cut(s) 144, 270, 327, 429, 718, 856, 1036, 1359
TaqII GACCGA 1 cut(s) 21
TasI AATT 3 cut(s) 446, 549, 770
TatI WGTACW 2 cut(s) 686, 1287
TauI GCSGC 2 cut(s) 69, 170
TfiI GAWTC 5 cut(s) 219, 329, 367, 610, 668
Tru1I TTAA 1 cut(s) 1065
Tru9I TTAA 1 cut(s) 1065
TscAI CASTG 3 cut(s) 741, 784, 982
TseFI GTSAC 2 cut(s) 73, 1406
TseI GCWGC 3 cut(s) 454, 599, 1157
Tsp45I GTSAC 2 cut(s) 73, 1406
TspDTI ATGAA 2 cut(s) 782, 1224
TspGWI ACGGA 6 cut(s) 114, 145, 360, 561, 895, 963
TspRI CASTG 3 cut(s) 741, 784, 982
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 9 cut(s) 4, 14, 55, 154, 176, 1100, 1322, 1336, 1375
XapI RAATTY 2 cut(s) 549, 770
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 1428
XmnI GAANNNNTTC 1 cut(s) 773
XspI CTAG 6 cut(s) 503, 578, 860, 960, 1074, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.