Rh5CG057300

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008029
Physical Location & Seq
Forward (+)
4224745 .. 4229996
5252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG057300.1

Sequence Viewer

Length: 1458 bp
ATGGACCGACCCGACTCCGACTCCGACTCCTCCTCCCGGCCCGTACTCTCCGACCGGGTCCTCATAAACGGCGCCGTAACGCCGGTCACGTTCTTCGCCGACGGCGTGCTGCGGTGGACGGAGAGAGGCGAGAGGAGCTTGATCGTCGAGAAGGAGGTCCTCGGGGTCAAAGTGGAGGGTCCGAAGATCAGAATCGGAGCTCTGGTCGACGAAGGAGGTGGAATCTGCTGCGTCGGCAGTAGCGGAGGAGCTCTGGTCAGGAAGGACTTGGTGTTCGAGCCTTTATCAGAGGACTCGCAGAGGCTCTGGTGCCAGAAGCTTCTCGAATTCATCGATTCTCTCGGACGGCCGAAGAGGCTGTTTGTGTTTGTGAATCCATTTGGAGGGAGCAAATCGGCTTCGAAGATTTTTGTTGATGAAGTGAAGCCGCTTTTTGATGATGCTGGTGTTCAGTTTACACTGCAAGAAACTCAGTATCAACTGCACGCGAAGGAAGTTGCGAAAACTCTAGATCTTTCGAAGTATGATGGCATTGTTTGCGTCAGTGGGGATGGAATCTTGGTTGAGGTAGTAAATGGACTTCTTGAGAGAGAGGATTGGGACACTGCAATAAAGATGCCTCTCGGAGTAGTTCCTGCAGGTACTGGAAATGGAATGGCGAAATCTCTTCTAGATTCTGTTGGTGAACCTTGTAAAGTATCAAATGCTGTTCTCGCCATTATTAGAGGACATAAGCGTTCATTGGATGTAGCTACCATATGGCAGGGGAAGACAAAATTTTTTAGTGTGTTGATGCTCGCATGGGGCTTAGTAGCAGATGTTGACATTGAGTCTGAAAAGTATAGGTGGATGGGAAGTGCTCGTCTAGATTTCTATGCCATTCAACGAATTGTGCATTTGAGGCAATATAACGGATCTATTTCTTTTGTGCCTGCACCTGGCTTCGAAGGGTTTGGAGAGCCTACTAGTTACAATGACGATGATGAATCTACTAACACTGATCCAAGTCAAGAAAAGCCTACTAAGGTTCGACAACTTGGCTATGAGGGACCTGATATTAATCTAAAGAACATGAACTGGAGAACAATCAAAGGACCGTTTGTTTCAGTGTGGCTTCACAATGTACCTTGGGGAGGTGAAGACACAAGGGCAGCTCCTGATGCCAAGTTCTCAGATGGTTATCTAGATGTTATAATTATGAGGGCTTGCCCAAAATTGTCCTTGCTCTCGTTAATGACGGGGTTGAGCACTGGGACTCATGTCAAGTCACCATATGTGCTATACTTCAAGGTGAAAGCTTTCGTTCTAGAGCCTGGTGCTCGCGCTGATGATTCAACGAAGGAAGGGATAGTGGACTCAGATGGTGAGGTCCTCGCTAGGGGCAAAGGAGCATACAAATGTCATCAGAAAACACTAATGGTGTACGATAAACTTCAAATAACTTTGGATCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

485

Amino Acids

53.31

Weight (kDa)

5.42

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 119 - 252 1.4e-31 Diacylglycerol kinase catalytic domain
YegS_C PF19279 368 - 472 2.5e-12 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1193
Acc36I ACCTGC 1 cut(s) 629
AccB1I GGYRCC 2 cut(s) 71, 309
AccI GTMKAC 1 cut(s) 207
AccII CGCG 2 cut(s) 488, 1323
AciI CCGC 3 cut(s) 112, 243, 428
AclWI GGATC 3 cut(s) 922, 995, 1455
AcoI YGGCCR 1 cut(s) 347
AcsI RAATTY 2 cut(s) 326, 776
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 4 cut(s) 45, 643, 1125, 1424
AfiI CCNNNNNNNGG 5 cut(s) 36, 383, 938, 1133, 1378
AgsI TTSAA 4 cut(s) 884, 1288, 1335, 1436
AhdI GACNNNNNGTC 1 cut(s) 829
AhlI ACTAGT 1 cut(s) 965
AjnI CCWGG 2 cut(s) 937, 1312
AloI GAACNNNNNNTCC 2 cut(s) 257, 289
AluBI AGCT 7 cut(s) 138, 200, 251, 319, 752, 1154, 1298
AluI AGCT 7 cut(s) 138, 200, 251, 319, 752, 1154, 1298
Alw21I GWGCWC 5 cut(s) 202, 253, 862, 1250, 1321
AlwI GGATC 3 cut(s) 922, 995, 1455
AlwNI CAGNNNCTG 2 cut(s) 644, 1157
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 2 cut(s) 38, 347
ApeKI GCWGC 3 cut(s) 109, 228, 1151
ApoI RAATTY 2 cut(s) 326, 776
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AseI ATTAAT 1 cut(s) 1059
Asp700I GAANNNNTTC 1 cut(s) 1298
AspLEI GCGC 2 cut(s) 74, 1325
AspS9I GGNCC 8 cut(s) 4, 39, 58, 157, 179, 1049, 1094, 1369
AsuC2I CCSGG 2 cut(s) 37, 56
AsuHPI GGTGA 5 cut(s) 695, 1148, 1260, 1303, 1376
AsuII TTCGAA 3 cut(s) 401, 518, 945
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 7 cut(s) 4, 58, 157, 179, 1049, 1094, 1369
BanI GGYRCC 2 cut(s) 71, 309
BanII GRGCYC 2 cut(s) 202, 253
BarI GAAGNNNNNNTAC 2 cut(s) 564, 596
BbsI GAAGAC 2 cut(s) 776, 1146
Bbv12I GWGCWC 5 cut(s) 202, 253, 862, 1250, 1321
BbvI GCAGC 3 cut(s) 96, 215, 1163
BccI CCATC 5 cut(s) 521, 545, 844, 1169, 1355
BceAI ACGGC 4 cut(s) 59, 85, 118, 362
BciT130I CCWGG 2 cut(s) 939, 1314
BcnI CCSGG 2 cut(s) 37, 56
BcuI ACTAGT 1 cut(s) 965
BfaI CTAG 7 cut(s) 509, 671, 866, 966, 1184, 1307, 1377
BfmI CTRYAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 629
BglI GCCNNNNNGGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 511
BisI GCNGC 4 cut(s) 110, 229, 428, 1152
BlsI GCNGC 4 cut(s) 111, 230, 429, 1153
Bme1390I CCNGG 4 cut(s) 37, 56, 939, 1314
Bme18I GGWCC 7 cut(s) 4, 58, 157, 179, 1049, 1094, 1369
BmeRI GACNNNNNGTC 1 cut(s) 829
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 8 cut(s) 4, 39, 58, 157, 179, 1049, 1094, 1369
BmiI GGNNCC 5 cut(s) 59, 73, 180, 311, 1050
BmrFI CCNGG 4 cut(s) 37, 56, 939, 1314
BmrI ACTGGG 1 cut(s) 1260
BmsI GCATC 4 cut(s) 430, 606, 783, 1150
BmuI ACTGGG 1 cut(s) 1260
BoxI GACNNNNGTC 1 cut(s) 1259
BpiI GAAGAC 2 cut(s) 776, 1146
BpmI CTGGAG 1 cut(s) 1099
Bpu14I TTCGAA 3 cut(s) 401, 518, 945
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 2 cut(s) 37, 56
Bsa29I ATCGAT 1 cut(s) 333
BsaBI GATNNNNATC 3 cut(s) 191, 1059, 1179
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 160, 1127
Bsc4I CCNNNNNNNGG 5 cut(s) 36, 383, 938, 1133, 1378
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 649, 1082, 1255
Bse8I GATNNNNATC 3 cut(s) 191, 1059, 1179
BseBI CCWGG 2 cut(s) 939, 1314
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 160, 1127
BseGI GGATG 3 cut(s) 556, 751, 855
BseJI GATNNNNATC 3 cut(s) 191, 1059, 1179
BseLI CCNNNNNNNGG 5 cut(s) 36, 383, 938, 1133, 1378
BseMII CTCAG 3 cut(s) 485, 1185, 1371
BseNI ACTGG 3 cut(s) 649, 1082, 1255
BseRI GAGGAG 4 cut(s) 19, 22, 148, 261
BseX3I CGGCCG 1 cut(s) 347
BseXI GCAGC 3 cut(s) 96, 215, 1163
BsgI GTGCAG 2 cut(s) 467, 918
Bsh1236I CGCG 2 cut(s) 488, 1323
Bsh1285I CGRYCG 2 cut(s) 55, 350
BshFI GGCC 2 cut(s) 40, 349
BshNI GGYRCC 2 cut(s) 71, 309
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 2 cut(s) 55, 350
BsiHKAI GWGCWC 5 cut(s) 202, 253, 862, 1250, 1321
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 37, 55, 83
BslFI GGGAC 3 cut(s) 614, 1062, 1267
BslI CCNNNNNNNGG 5 cut(s) 36, 383, 938, 1133, 1378
BsmFI GGGAC 3 cut(s) 614, 1062, 1267
BsnI GGCC 2 cut(s) 40, 349
BsoBI CYCGRG 1 cut(s) 161
Bsp119I TTCGAA 3 cut(s) 401, 518, 945
Bsp1286I GDGCHC 5 cut(s) 202, 253, 862, 1250, 1321
Bsp143I GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
BspACI CCGC 3 cut(s) 112, 243, 428
BspANI GGCC 2 cut(s) 40, 349
BspCNI CTCAG 3 cut(s) 484, 1184, 1370
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 2 cut(s) 488, 1323
BspLI GGNNCC 5 cut(s) 59, 73, 180, 311, 1050
BspMAI CTGCAG 1 cut(s) 640
BspMI ACCTGC 1 cut(s) 629
BspPI GGATC 3 cut(s) 922, 995, 1455
BspT104I TTCGAA 3 cut(s) 401, 518, 945
BspT107I GGYRCC 2 cut(s) 71, 309
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 3 cut(s) 649, 1082, 1255
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 160, 1127
BssMI GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 1127
Bst2UI CCWGG 2 cut(s) 939, 1314
Bst4CI ACNGT 1 cut(s) 1098
Bst6I CTCTTC 2 cut(s) 347, 672
BstACI GRCGYC 1 cut(s) 72
BstAPI GCANNNNNTGC 1 cut(s) 537
BstBI TTCGAA 3 cut(s) 401, 518, 945
BstC8I GCNNGC 6 cut(s) 107, 486, 798, 933, 1207, 1321
BstDEI CTNAG 5 cut(s) 471, 808, 1023, 1171, 1357
BstENI CCTNNNNNAGG 1 cut(s) 1131
BstF5I GGATG 3 cut(s) 556, 751, 855
BstFNI CGCG 2 cut(s) 488, 1323
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 74, 1325
BstKTI GATC 6 cut(s) 144, 189, 514, 917, 1003, 1450
BstMBI GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
BstMCI CGRYCG 2 cut(s) 55, 350
BstMWI GCNNNNNNNGC 7 cut(s) 135, 234, 355, 537, 713, 901, 1160
BstNI CCWGG 2 cut(s) 939, 1314
BstPAI GACNNNNGTC 1 cut(s) 1259
BstSCI CCNGG 4 cut(s) 35, 54, 937, 1312
BstSFI CTRYAG 1 cut(s) 636
BstUI CGCG 2 cut(s) 488, 1323
BstV1I GCAGC 3 cut(s) 96, 215, 1163
BstV2I GAAGAC 2 cut(s) 776, 1146
BstX2I RGATCY 2 cut(s) 511, 914
BstYI RGATCY 2 cut(s) 511, 914
BstZI CGGCCG 1 cut(s) 347
Bsu15I ATCGAT 1 cut(s) 333
BsuRI GGCC 2 cut(s) 40, 349
BsuTUI ATCGAT 1 cut(s) 333
BtsCI GGATG 3 cut(s) 556, 751, 855
BtsI GCAGTG 2 cut(s) 458, 603
BtsIMutI CAGTG 6 cut(s) 458, 550, 603, 996, 1113, 1248
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 6 cut(s) 107, 486, 798, 933, 1207, 1321
CaiI CAGNNNCTG 2 cut(s) 644, 1157
CfoI GCGC 2 cut(s) 74, 1325
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 8 cut(s) 4, 39, 58, 157, 179, 1049, 1094, 1369
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 2 cut(s) 220, 529
Csp6I GTAC 4 cut(s) 44, 642, 1124, 1423
CviAII CATG 3 cut(s) 801, 1072, 1259
CviQI GTAC 4 cut(s) 44, 642, 1124, 1423
DdeI CTNAG 5 cut(s) 471, 808, 1023, 1171, 1357
DinI GGCGCC 1 cut(s) 73
DpnI GATC 6 cut(s) 143, 188, 513, 916, 1002, 1449
DpnII GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
DriI GACNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 1 cut(s) 347
EagI CGGCCG 1 cut(s) 347
Eam1104I CTCTTC 2 cut(s) 347, 672
Eam1105I GACNNNNNGTC 1 cut(s) 829
EarI CTCTTC 2 cut(s) 347, 672
Ecl136II GAGCTC 2 cut(s) 200, 251
EclXI CGGCCG 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 1127
Eco24I GRGCYC 2 cut(s) 202, 253
Eco47I GGWCC 7 cut(s) 4, 58, 157, 179, 1049, 1094, 1369
Eco52I CGGCCG 1 cut(s) 347
Eco53kI GAGCTC 2 cut(s) 200, 251
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 2 cut(s) 200, 251
EcoNI CCTNNNNNAGG 1 cut(s) 1131
EcoO109I RGGNCCY 4 cut(s) 58, 157, 1049, 1369
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 937, 1312
EcoT14I CCWWGG 1 cut(s) 1127
EcoT38I GRGCYC 2 cut(s) 202, 253
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 1127
FaeI CATG 3 cut(s) 804, 1075, 1262
FaqI GGGAC 3 cut(s) 614, 1062, 1267
FatI CATG 3 cut(s) 800, 1071, 1258
FauNDI CATATG 2 cut(s) 758, 1273
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 110, 229, 428, 1152
FokI GGATG 3 cut(s) 563, 758, 862
FriOI GRGCYC 2 cut(s) 202, 253
Fsp4HI GCNGC 4 cut(s) 110, 229, 428, 1152
FspBI CTAG 7 cut(s) 509, 671, 866, 966, 1184, 1307, 1377
GlaI GCGC 2 cut(s) 73, 1324
GluI GCNGC 4 cut(s) 110, 229, 428, 1152
GsuI CTGGAG 1 cut(s) 1099
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 40, 349
HapII CCGG 3 cut(s) 37, 55, 83
HgaI GACGC 2 cut(s) 220, 529
HhaI GCGC 2 cut(s) 74, 1325
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 3 cut(s) 804, 1075, 1262
Hin6I GCGC 2 cut(s) 72, 1323
HinP1I GCGC 2 cut(s) 72, 1323
HincII GTYRAC 2 cut(s) 208, 823
HindII GTYRAC 2 cut(s) 208, 823
HindIII AAGCTT 2 cut(s) 317, 1296
HpaII CCGG 3 cut(s) 37, 55, 83
HphI GGTGA 5 cut(s) 695, 1148, 1260, 1303, 1376
Hpy166II GTNNAC 7 cut(s) 117, 208, 456, 686, 823, 1354, 1423
Hpy8I GTNNAC 7 cut(s) 117, 208, 456, 686, 823, 1354, 1423
Hpy99I CGWCG 4 cut(s) 104, 149, 212, 236
HpyAV CCTTC 7 cut(s) 145, 206, 256, 484, 941, 1333, 1337
HpyCH4III ACNGT 1 cut(s) 1098
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 6 cut(s) 463, 484, 608, 638, 895, 935
HpyF10VI GCNNNNNNNGC 7 cut(s) 135, 234, 355, 537, 713, 901, 1160
HpyF3I CTNAG 5 cut(s) 471, 808, 1023, 1171, 1357
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 3 cut(s) 804, 1075, 1262
HspAI GCGC 2 cut(s) 72, 1323
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
LmnI GCTCC 6 cut(s) 135, 197, 248, 387, 1159, 1388
Lsp1109I GCAGC 3 cut(s) 96, 215, 1163
LweI GCATC 4 cut(s) 430, 606, 783, 1150
MaeI CTAG 7 cut(s) 509, 671, 866, 966, 1184, 1307, 1377
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 4 cut(s) 76, 85, 968, 1266
MalI GATC 6 cut(s) 143, 188, 513, 916, 1002, 1449
MboI GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
MboII GAAGA 7 cut(s) 85, 196, 364, 415, 659, 781, 1151
MflI RGATCY 2 cut(s) 511, 914
MhlI GDGCHC 5 cut(s) 202, 253, 862, 1250, 1321
MluCI AATT 5 cut(s) 326, 776, 888, 1194, 1214
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 7 cut(s) 8, 14, 20, 287, 839, 1249, 1349
MmeI TCCRAC 3 cut(s) 42, 48, 75
MroXI GAANNNNTTC 1 cut(s) 1298
MseI TTAA 2 cut(s) 1059, 1232
MslI CAYNNNNRTG 1 cut(s) 1396
MspI CCGG 3 cut(s) 37, 55, 83
MspR9I CCNGG 4 cut(s) 37, 56, 939, 1314
MvaI CCWGG 2 cut(s) 939, 1314
MvnI CGCG 2 cut(s) 488, 1323
MwoI GCNNNNNNNGC 7 cut(s) 135, 234, 355, 537, 713, 901, 1160
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 2 cut(s) 37, 56
NdeI CATATG 2 cut(s) 758, 1273
NdeII GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
NlaIII CATG 3 cut(s) 804, 1075, 1262
NlaIV GGNNCC 5 cut(s) 59, 73, 180, 311, 1050
NmuCI GTSAC 2 cut(s) 85, 1266
NspV TTCGAA 3 cut(s) 401, 518, 945
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 102, 330
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 8 cut(s) 192, 222, 335, 373, 555, 674, 986, 1331
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 111, 230, 429, 1153
PleI GAGTC 7 cut(s) 8, 14, 20, 287, 838, 1249, 1349
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 7 cut(s) 8, 14, 20, 287, 838, 1249, 1349
PpuMI RGGWCCY 4 cut(s) 58, 157, 1049, 1369
PshAI GACNNNNGTC 1 cut(s) 1259
PshBI ATTAAT 1 cut(s) 1059
PsiI TTATAA 1 cut(s) 1193
Psp124BI GAGCTC 2 cut(s) 202, 253
Psp5II RGGWCCY 4 cut(s) 58, 157, 1049, 1369
Psp6I CCWGG 2 cut(s) 937, 1312
PspGI CCWGG 2 cut(s) 937, 1312
PspN4I GGNNCC 5 cut(s) 59, 73, 180, 311, 1050
PspPI GGNCC 8 cut(s) 4, 39, 58, 157, 179, 1049, 1094, 1369
PspPPI RGGWCCY 4 cut(s) 58, 157, 1049, 1369
PstI CTGCAG 1 cut(s) 640
PstNI CAGNNNCTG 2 cut(s) 644, 1157
PsuI RGATCY 2 cut(s) 511, 914
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 4 cut(s) 45, 643, 1125, 1424
RsaNI GTAC 4 cut(s) 44, 642, 1124, 1423
RseI CAYNNNNRTG 1 cut(s) 1396
SacI GAGCTC 2 cut(s) 202, 253
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 2 cut(s) 1059, 1232
SatI GCNGC 4 cut(s) 110, 229, 428, 1152
Sau3AI GATC 6 cut(s) 141, 186, 511, 914, 1000, 1447
Sau96I GGNCC 8 cut(s) 4, 39, 58, 157, 179, 1049, 1094, 1369
SbfI CCTGCAGG 1 cut(s) 640
SchI GAGTC 7 cut(s) 8, 14, 20, 287, 839, 1249, 1349
ScrFI CCNGG 4 cut(s) 37, 56, 939, 1314
SdaI CCTGCAGG 1 cut(s) 640
SduI GDGCHC 5 cut(s) 202, 253, 862, 1250, 1321
SfaNI GCATC 4 cut(s) 430, 606, 783, 1150
SfcI CTRYAG 1 cut(s) 636
SfoI GGCGCC 1 cut(s) 73
SfuI TTCGAA 3 cut(s) 401, 518, 945
SinI GGWCC 7 cut(s) 4, 58, 157, 179, 1049, 1094, 1369
SmiMI CAYNNNNRTG 1 cut(s) 1396
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SpeI ACTAGT 1 cut(s) 965
Sse8387I CCTGCAGG 1 cut(s) 640
Sse9I AATT 5 cut(s) 326, 776, 888, 1194, 1214
SsiI CCGC 3 cut(s) 112, 243, 428
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 7 cut(s) 509, 671, 866, 966, 1184, 1307, 1377
SstI GAGCTC 2 cut(s) 202, 253
StyD4I CCNGG 4 cut(s) 35, 54, 937, 1312
StyI CCWWGG 1 cut(s) 1127
TaaI ACNGT 1 cut(s) 1098
TaiI ACGT 1 cut(s) 92
TaqI TCGA 9 cut(s) 147, 207, 276, 324, 333, 401, 518, 945, 1030
TaqII GACCGA 1 cut(s) 21
TasI AATT 5 cut(s) 326, 776, 888, 1194, 1214
TauI GCSGC 1 cut(s) 430
TfiI GAWTC 8 cut(s) 192, 222, 335, 373, 555, 674, 986, 1331
Tru1I TTAA 2 cut(s) 1059, 1232
Tru9I TTAA 2 cut(s) 1059, 1232
TscAI CASTG 6 cut(s) 465, 550, 610, 1003, 1113, 1255
TseFI GTSAC 2 cut(s) 85, 1266
TseI GCWGC 3 cut(s) 109, 228, 1151
Tsp45I GTSAC 2 cut(s) 85, 1266
TspDTI ATGAA 5 cut(s) 319, 432, 729, 999, 1088
TspGWI ACGGA 2 cut(s) 134, 927
TspRI CASTG 6 cut(s) 465, 550, 610, 1003, 1113, 1255
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 7 cut(s) 4, 58, 157, 179, 1049, 1094, 1369
VspI ATTAAT 1 cut(s) 1059
XagI CCTNNNNNAGG 1 cut(s) 1131
XapI RAATTY 2 cut(s) 326, 776
XbaI TCTAGA 5 cut(s) 508, 670, 865, 1183, 1306
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 7 cut(s) 509, 671, 866, 966, 1184, 1307, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.