MD10G1302700.v1.1

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008029
Physical Location & Seq
Reverse (-)
38935011 .. 38939570
4560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1302700.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGACCGACCCGGACGCGTACCCGAGTCCGCTCCACCAGAATCCCTTATCTCCGACCGGGTTCTCATAAACGGCGTTGTTACACCGGTGACTCTATTCTCCGACGGAGTGCTCCAGTGGTCGCAGAGAGGTCACCGGAGCTTGATCGTCGAGAAGGAGGTCCTCGGGTTCGCGGCAGAAGGCCCGAAGATTAGGATAAGAGCTTTAGTTGAAGGCCGAGCTGGAATCTGCTGCGCCGCGAGCAGAGGAGCTGTGGCGCGGAAGGACTTGGTGTTCGAGCCTCTCTTTGAGGAATCGCACCGGCTCTGGTGCCAGAAGCTCCGTGATTACATCGATTCTCTCGGACGGCCAAAGAGGCTGTTTGTTTTTGTGAATCCGTTTGGTGGGAGCAAATCTGCTTCAAAGATTTATCTTGATCACGTAAAACCTCTTTTCGAGGATGCCGGCATCCAATTTACGCTGCAAGAAACTCGATATCAACTACATGCGAAGGAAGTTGCTAAAACACTAGATCTTTCAAAGTATGATGGCGTTGTTTGTGTTAGTGGGGATGGAATCTTGGTTGAGGTTAGTTTGCAGTTATCGGTGGCAGCCATGATAAACACGAGCAATGCCATGTTGGAGAGCTGCGGTAATTTGGTAGTAAATGGATTCCTTGAGAGAGAGGATTGGGATGCTGCAATAAAGATCCCCCTTGGAGTAGTTCCTGCAGGTTTCGGGTGGTACTGGAAATGGAATGGCAAATCTCTTTCGGATTCTGTTGGTGAACCTTGTACACCTTCTAATGCTGTTCTCGCCATTATTCGAGGGCATAAGCGTTCACTGGATGTAGCTACCATATGCCAGGGGAAGACCAAATTCTTTAGTGTATTGATGCTTGCATGGGGTTTAGTGGCAGATATTGACATTGAGTCTGAGAAGTATAGGTGGATGGGAAGTGCTCGACTAGATTTCTATGCTGTTCAACGGATTGTGCGTTTGAGGCAATATCATGGATCTATTTCGTTTGTGCCTGCACCGGGCTTTGAAACTTACGGAGAGCCGACTAGTTACAGTAATGACAGTGAACCTGCTAACCAACACATTGCATGTGCTCCAAATCAAGAGGAACCGATTAACATTCGACAGCATGGCTATGAGGGGCCTGATATTAACTTGGATAGCATGGACTGGAGAACTATTAGGGGGCCCTTCGTTTCAATCTGGCTTCACAATGTGCCTTGGGGTGGTGAAGACACAAAGGCAGCGCCTGATGCCAAGTTCTCGGATGGTTATATAGACGTGATATTGATGAGGGCCTGTCCAAAATTATCATTGCTCTCGTTGATGGCTGGGTTGAGCACCGGAAGCCATATCAAATCACCATATGTCTTGTACTTCAAGGTGAAAGCATTTATTTTGCAACCTGGTCCTCGCACCGAGGACCCAGTTAAGGGAGGGATAATCGACTCAGATGGCGAGGTCCTGGCTAGAGGCAGAGGAGCATACAAATGTGACCAAAAGACACTGATGACGTACGACGAACTTCAAATAACCGTGGATCAAGGTTTAGCTACTCTGTTTTCCCCAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

525

Amino Acids

57.89

Weight (kDa)

6.25

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAGK_cat PF00781 119 - 279 4.6e-19 Diacylglycerol kinase catalytic domain
YegS_C PF19279 400 - 491 9.5e-10 YegS C-terminal NAD kinase beta sandwich-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 701, 1078
AccB1I GGYRCC 1 cut(s) 309
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 4 cut(s) 18, 173, 239, 259
AciI CCGC 5 cut(s) 30, 173, 237, 259, 630
AclWI GGATC 3 cut(s) 682, 1003, 1548
AcoI YGGCCR 1 cut(s) 347
AcsI RAATTY 1 cut(s) 857
AdeI CACNNNGTG 1 cut(s) 1216
AfaI GTAC 5 cut(s) 21, 725, 775, 1376, 1517
AfiI CCNNNNNNNGG 5 cut(s) 383, 1019, 1226, 1432, 1433
AflIII ACRYGT 1 cut(s) 16
AgeI ACCGGT 1 cut(s) 85
AgsI TTSAA 8 cut(s) 212, 402, 519, 965, 1028, 1200, 1381, 1529
AhdI GACNNNNNGTC 1 cut(s) 910
AhlI ACTAGT 1 cut(s) 1046
AjiI CACGTC 1 cut(s) 1282
AjnI CCWGG 3 cut(s) 843, 1405, 1464
AloI GAACNNNNNNTCC 2 cut(s) 257, 289
AluBI AGCT 8 cut(s) 141, 203, 221, 251, 319, 627, 833, 1553
AluI AGCT 8 cut(s) 141, 203, 221, 251, 319, 627, 833, 1553
Alw21I GWGCWC 4 cut(s) 114, 943, 1096, 1343
AlwI GGATC 3 cut(s) 682, 1003, 1548
AlwNI CAGNNNCTG 1 cut(s) 1250
Ama87I CYCGRG 2 cut(s) 23, 164
AoxI GGCC 6 cut(s) 181, 214, 347, 1142, 1187, 1296
ApaI GGGCCC 1 cut(s) 1191
ApeKI GCWGC 6 cut(s) 231, 460, 590, 627, 677, 1244
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AsiGI ACCGGT 1 cut(s) 85
AspLEI GCGC 3 cut(s) 236, 259, 1249
AsuC2I CCSGG 3 cut(s) 12, 59, 1020
AsuHPI GGTGA 6 cut(s) 100, 125, 776, 1241, 1353, 1396
AvaI CYCGRG 2 cut(s) 23, 164
AvaII GGWCC 5 cut(s) 4, 160, 1409, 1423, 1462
BaeGI GKGCMC 1 cut(s) 1191
BanI GGYRCC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 1191
BauI CACGAG 1 cut(s) 604
BbsI GAAGAC 2 cut(s) 857, 1239
Bbv12I GWGCWC 4 cut(s) 114, 943, 1096, 1343
BbvI GCAGC 6 cut(s) 218, 447, 602, 614, 664, 1256
BccI CCATC 6 cut(s) 521, 545, 925, 1262, 1321, 1448
BceAI ACGGC 2 cut(s) 88, 362
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 3 cut(s) 845, 1407, 1466
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 3 cut(s) 12, 59, 1020
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 4 cut(s) 509, 947, 1047, 1470
BfmI CTRYAG 1 cut(s) 708
BfoI RGCGCY 1 cut(s) 1250
BfuAI ACCTGC 2 cut(s) 701, 1078
BglI GCCNNNNNGGC 1 cut(s) 355
BglII AGATCT 1 cut(s) 511
BisI GCNGC 8 cut(s) 174, 232, 237, 461, 591, 628, 678, 1245
BlsI GCNGC 8 cut(s) 175, 233, 238, 462, 592, 629, 679, 1246
Bme1390I CCNGG 6 cut(s) 12, 59, 845, 1020, 1407, 1466
Bme18I GGWCC 5 cut(s) 4, 160, 1409, 1423, 1462
BmeRI GACNNNNNGTC 1 cut(s) 910
BmeT110I CYCGRG 2 cut(s) 23, 164
BmgBI CACGTC 1 cut(s) 1282
BmiI GGNNCC 6 cut(s) 311, 1110, 1143, 1188, 1189, 1425
BmrFI CCNGG 6 cut(s) 12, 59, 845, 1020, 1407, 1466
BmrI ACTGGG 2 cut(s) 1421, 1562
BmsI GCATC 5 cut(s) 430, 456, 664, 864, 1243
BmuI ACTGGG 2 cut(s) 1421, 1562
BpiI GAAGAC 2 cut(s) 857, 1239
BpmI CTGGAG 2 cut(s) 98, 1192
BpuEI CTTGAG 1 cut(s) 677
BpuMI CCSGG 3 cut(s) 12, 59, 1020
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 421
BsaJI CCNNGG 6 cut(s) 163, 694, 844, 1220, 1419, 1536
BsaWI WCCGGW 3 cut(s) 85, 135, 1343
Bsc4I CCNNNNNNNGG 5 cut(s) 383, 1019, 1226, 1432, 1433
Bse118I RCCGGY 3 cut(s) 85, 300, 443
Bse1I ACTGG 6 cut(s) 115, 731, 828, 1175, 1427, 1568
Bse3DI GCAATG 3 cut(s) 616, 1083, 1313
BseBI CCWGG 3 cut(s) 845, 1407, 1466
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 6 cut(s) 163, 694, 844, 1220, 1419, 1536
BseGI GGATG 7 cut(s) 445, 447, 556, 679, 832, 936, 1273
BseLI CCNNNNNNNGG 5 cut(s) 383, 1019, 1226, 1432, 1433
BseMI GCAATG 3 cut(s) 616, 1083, 1313
BseMII CTCAG 2 cut(s) 906, 1464
BseNI ACTGG 6 cut(s) 115, 731, 828, 1175, 1427, 1568
BseRI GAGGAG 2 cut(s) 261, 1494
BseSI GKGCMC 1 cut(s) 1191
BseXI GCAGC 6 cut(s) 218, 447, 602, 614, 664, 1256
BseYI CCCAGC 1 cut(s) 1331
BsgI GTGCAG 1 cut(s) 999
Bsh1236I CGCG 4 cut(s) 18, 173, 239, 259
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 6 cut(s) 183, 216, 349, 1144, 1189, 1298
BshNI GGYRCC 1 cut(s) 309
BshTI ACCGGT 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 4 cut(s) 114, 943, 1096, 1343
BsiHKCI CYCGRG 2 cut(s) 23, 164
BsiSI CCGG 8 cut(s) 12, 58, 86, 136, 301, 444, 1019, 1344
BsiWI CGTACG 1 cut(s) 1515
BslI CCNNNNNNNGG 5 cut(s) 383, 1019, 1226, 1432, 1433
BsnI GGCC 6 cut(s) 183, 216, 349, 1144, 1189, 1298
BsoBI CYCGRG 2 cut(s) 23, 164
Bsp120I GGGCCC 1 cut(s) 1187
Bsp1286I GDGCHC 5 cut(s) 114, 943, 1096, 1191, 1343
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
BspACI CCGC 5 cut(s) 30, 173, 237, 259, 630
BspANI GGCC 6 cut(s) 183, 216, 349, 1144, 1189, 1298
BspCNI CTCAG 2 cut(s) 907, 1463
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 4 cut(s) 18, 173, 239, 259
BspLI GGNNCC 6 cut(s) 311, 1110, 1143, 1188, 1189, 1425
BspMAI CTGCAG 1 cut(s) 712
BspMI ACCTGC 2 cut(s) 701, 1078
BspPI GGATC 3 cut(s) 682, 1003, 1548
BspT107I GGYRCC 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 32
BsrDI GCAATG 3 cut(s) 616, 1083, 1313
BsrFI RCCGGY 3 cut(s) 85, 300, 443
BsrGI TGTACA 1 cut(s) 773
BsrI ACTGG 6 cut(s) 115, 731, 828, 1175, 1427, 1568
BssAI RCCGGY 3 cut(s) 85, 300, 443
BssECI CCNNGG 6 cut(s) 163, 694, 844, 1220, 1419, 1536
BssMI GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
BssSI CACGAG 1 cut(s) 604
BssT1I CCWWGG 2 cut(s) 694, 1220
Bst2BI CACGAG 1 cut(s) 604
Bst2UI CCWGG 3 cut(s) 845, 1407, 1466
Bst4CI ACNGT 3 cut(s) 1055, 1064, 1537
BstAUI TGTACA 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 4 cut(s) 241, 445, 879, 1014
BstDEI CTNAG 2 cut(s) 915, 1450
BstDSI CCRYGG 1 cut(s) 1536
BstEII GGTNACC 1 cut(s) 131
BstF5I GGATG 7 cut(s) 445, 447, 556, 679, 832, 936, 1273
BstFNI CGCG 4 cut(s) 18, 173, 239, 259
BstH2I RGCGCY 1 cut(s) 1250
BstHHI GCGC 3 cut(s) 236, 259, 1249
BstKTI GATC 6 cut(s) 147, 418, 514, 690, 998, 1543
BstMBI GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 6 cut(s) 240, 355, 794, 982, 1253, 1347
BstNI CCWGG 3 cut(s) 845, 1407, 1466
BstNSI RCATGY 2 cut(s) 488, 1092
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 6 cut(s) 10, 57, 843, 1018, 1405, 1464
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 1191
BstUI CGCG 4 cut(s) 18, 173, 239, 259
BstV1I GCAGC 6 cut(s) 218, 447, 602, 614, 664, 1256
BstV2I GAAGAC 2 cut(s) 857, 1239
BstX2I RGATCY 3 cut(s) 511, 687, 995
BstYI RGATCY 3 cut(s) 511, 687, 995
Bsu15I ATCGAT 1 cut(s) 333
BsuRI GGCC 6 cut(s) 183, 216, 349, 1144, 1189, 1298
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 1536
BtrI CACGTC 1 cut(s) 1282
BtsCI GGATG 7 cut(s) 445, 447, 556, 679, 832, 936, 1273
BtsIMutI CAGTG 4 cut(s) 122, 821, 1069, 1505
BveI ACCTGC 2 cut(s) 701, 1078
Cac8I GCNNGC 4 cut(s) 241, 445, 879, 1014
CaiI CAGNNNCTG 1 cut(s) 1250
CfoI GCGC 3 cut(s) 236, 259, 1249
Cfr10I RCCGGY 3 cut(s) 85, 300, 443
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 1405
Csp6I GTAC 5 cut(s) 20, 724, 774, 1375, 1516
CspAI ACCGGT 1 cut(s) 85
CviAII CATG 8 cut(s) 485, 595, 616, 882, 992, 1089, 1130, 1165
CviQI GTAC 5 cut(s) 20, 724, 774, 1375, 1516
DdeI CTNAG 2 cut(s) 915, 1450
DpnI GATC 6 cut(s) 146, 417, 513, 689, 997, 1542
DpnII GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
DraIII CACNNNGTG 1 cut(s) 1216
DriI GACNNNNNGTC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 347
Eam1105I GACNNNNNGTC 1 cut(s) 910
Eco130I CCWWGG 2 cut(s) 694, 1220
Eco24I GRGCYC 1 cut(s) 1191
Eco32I GATATC 1 cut(s) 476
Eco47I GGWCC 5 cut(s) 4, 160, 1409, 1423, 1462
Eco88I CYCGRG 2 cut(s) 23, 164
Eco91I GGTNACC 1 cut(s) 131
EcoO109I RGGNCCY 7 cut(s) 160, 1142, 1187, 1188, 1296, 1423, 1462
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 843, 1405, 1464
EcoRV GATATC 1 cut(s) 476
EcoT14I CCWWGG 2 cut(s) 694, 1220
EcoT38I GRGCYC 1 cut(s) 1191
ErhI CCWWGG 2 cut(s) 694, 1220
FaeI CATG 8 cut(s) 488, 598, 619, 885, 995, 1092, 1133, 1168
FatI CATG 8 cut(s) 484, 594, 615, 881, 991, 1088, 1129, 1164
FauNDI CATATG 2 cut(s) 839, 1366
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 8 cut(s) 174, 232, 237, 461, 591, 628, 678, 1245
FokI GGATG 7 cut(s) 434, 452, 563, 686, 839, 943, 1280
FriOI GRGCYC 1 cut(s) 1191
Fsp4HI GCNGC 8 cut(s) 174, 232, 237, 461, 591, 628, 678, 1245
FspBI CTAG 4 cut(s) 509, 947, 1047, 1470
GlaI GCGC 3 cut(s) 235, 258, 1248
GluI GCNGC 8 cut(s) 174, 232, 237, 461, 591, 628, 678, 1245
GsaI CCCAGC 1 cut(s) 1335
GsuI CTGGAG 2 cut(s) 98, 1192
HaeII RGCGCY 1 cut(s) 1250
HaeIII GGCC 6 cut(s) 183, 216, 349, 1144, 1189, 1298
HapII CCGG 8 cut(s) 12, 58, 86, 136, 301, 444, 1019, 1344
HgaI GACGC 1 cut(s) 24
HhaI GCGC 3 cut(s) 236, 259, 1249
Hin1II CATG 8 cut(s) 488, 598, 619, 885, 995, 1092, 1133, 1168
Hin6I GCGC 3 cut(s) 234, 257, 1247
HinP1I GCGC 3 cut(s) 234, 257, 1247
HpaII CCGG 8 cut(s) 12, 58, 86, 136, 301, 444, 1019, 1344
HphI GGTGA 6 cut(s) 100, 125, 776, 1241, 1353, 1396
Hpy166II GTNNAC 4 cut(s) 767, 776, 821, 1067
Hpy188I TCNGA 7 cut(s) 55, 103, 344, 754, 916, 1267, 1453
Hpy188III TCNNGA 3 cut(s) 151, 413, 1103
Hpy8I GTNNAC 4 cut(s) 767, 776, 821, 1067
Hpy99I CGWCG 3 cut(s) 107, 152, 1523
HpyAV CCTTC 7 cut(s) 148, 173, 206, 256, 484, 789, 1201
HpyCH4III ACNGT 3 cut(s) 1055, 1064, 1537
HpyCH4IV ACGT 3 cut(s) 420, 1281, 1514
HpyCH4V TGCA 8 cut(s) 463, 577, 680, 710, 881, 1016, 1088, 1402
HpyF10VI GCNNNNNNNGC 6 cut(s) 240, 355, 794, 982, 1253, 1347
HpyF3I CTNAG 2 cut(s) 915, 1450
HpySE526I ACGT 3 cut(s) 420, 1281, 1514
Hsp92II CATG 8 cut(s) 488, 598, 619, 885, 995, 1092, 1133, 1168
HspAI GCGC 3 cut(s) 234, 257, 1247
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
LmnI GCTCC 8 cut(s) 37, 117, 138, 248, 324, 387, 1099, 1481
Lsp1109I GCAGC 6 cut(s) 218, 447, 602, 614, 664, 1256
LweI GCATC 5 cut(s) 430, 456, 664, 864, 1243
MabI ACCWGGT 1 cut(s) 1405
MaeI CTAG 4 cut(s) 509, 947, 1047, 1470
MaeII ACGT 3 cut(s) 420, 1281, 1514
MaeIII GTNAC 5 cut(s) 79, 88, 131, 1049, 1493
MalI GATC 6 cut(s) 146, 417, 513, 689, 997, 1542
MbiI CCGCTC 1 cut(s) 32
MboI GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
MboII GAAGA 3 cut(s) 199, 862, 1244
MflI RGATCY 3 cut(s) 511, 687, 995
MhlI GDGCHC 5 cut(s) 114, 943, 1096, 1191, 1343
MluCI AATT 4 cut(s) 452, 634, 857, 1307
MluI ACGCGT 1 cut(s) 16
MlyI GAGTC 4 cut(s) 35, 85, 920, 1442
MmeI TCCRAC 3 cut(s) 78, 126, 600
MroNI GCCGGC 1 cut(s) 443
MseI TTAA 3 cut(s) 1116, 1152, 1431
MslI CAYNNNNRTG 2 cut(s) 1134, 1489
MspI CCGG 8 cut(s) 12, 58, 86, 136, 301, 444, 1019, 1344
MspR9I CCNGG 6 cut(s) 12, 59, 845, 1020, 1407, 1466
MvaI CCWGG 3 cut(s) 845, 1407, 1466
MvnI CGCG 4 cut(s) 18, 173, 239, 259
MwoI GCNNNNNNNGC 6 cut(s) 240, 355, 794, 982, 1253, 1347
NaeI GCCGGC 1 cut(s) 445
NciI CCSGG 3 cut(s) 12, 59, 1020
NdeI CATATG 2 cut(s) 839, 1366
NdeII GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
NgoMIV GCCGGC 1 cut(s) 443
NlaIII CATG 8 cut(s) 488, 598, 619, 885, 995, 1092, 1133, 1168
NlaIV GGNNCC 6 cut(s) 311, 1110, 1143, 1188, 1189, 1425
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 3 cut(s) 88, 131, 1493
NspI RCATGY 2 cut(s) 488, 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 973
PdiI GCCGGC 1 cut(s) 445
PfeI GAWTC 8 cut(s) 41, 225, 293, 335, 373, 555, 651, 755
Pfl23II CGTACG 1 cut(s) 1515
PinAI ACCGGT 1 cut(s) 85
PkrI GCNGC 8 cut(s) 175, 233, 238, 462, 592, 629, 679, 1246
PleI GAGTC 4 cut(s) 34, 85, 919, 1442
PpsI GAGTC 4 cut(s) 34, 85, 919, 1442
Ppu21I YACGTR 1 cut(s) 421
PpuMI RGGWCCY 3 cut(s) 160, 1423, 1462
Psp5II RGGWCCY 3 cut(s) 160, 1423, 1462
Psp6I CCWGG 3 cut(s) 843, 1405, 1464
PspEI GGTNACC 1 cut(s) 131
PspFI CCCAGC 1 cut(s) 1331
PspGI CCWGG 3 cut(s) 843, 1405, 1464
PspLI CGTACG 1 cut(s) 1515
PspN4I GGNNCC 6 cut(s) 311, 1110, 1143, 1188, 1189, 1425
PspOMI GGGCCC 1 cut(s) 1187
PspPPI RGGWCCY 3 cut(s) 160, 1423, 1462
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 1250
PsuI RGATCY 3 cut(s) 511, 687, 995
RsaI GTAC 5 cut(s) 21, 725, 775, 1376, 1517
RsaNI GTAC 5 cut(s) 20, 724, 774, 1375, 1516
RseI CAYNNNNRTG 2 cut(s) 1134, 1489
SaqAI TTAA 3 cut(s) 1116, 1152, 1431
SatI GCNGC 8 cut(s) 174, 232, 237, 461, 591, 628, 678, 1245
Sau3AI GATC 6 cut(s) 144, 415, 511, 687, 995, 1540
SbfI CCTGCAGG 1 cut(s) 712
SchI GAGTC 4 cut(s) 35, 85, 920, 1442
ScrFI CCNGG 6 cut(s) 12, 59, 845, 1020, 1407, 1466
SdaI CCTGCAGG 1 cut(s) 712
SduI GDGCHC 5 cut(s) 114, 943, 1096, 1191, 1343
SexAI ACCWGGT 1 cut(s) 1405
SfaNI GCATC 5 cut(s) 430, 456, 664, 864, 1243
SfcI CTRYAG 1 cut(s) 708
SgrAI CRCCGGYG 1 cut(s) 85
SinI GGWCC 5 cut(s) 4, 160, 1409, 1423, 1462
SmiMI CAYNNNNRTG 2 cut(s) 1134, 1489
SmlI CTYRAG 1 cut(s) 656
SmoI CTYRAG 1 cut(s) 656
SpeI ACTAGT 1 cut(s) 1046
Sse8387I CCTGCAGG 1 cut(s) 712
Sse9I AATT 4 cut(s) 452, 634, 857, 1307
SsiI CCGC 5 cut(s) 30, 173, 237, 259, 630
SspMI CTAG 4 cut(s) 509, 947, 1047, 1470
StyD4I CCNGG 6 cut(s) 10, 57, 843, 1018, 1405, 1464
StyI CCWWGG 2 cut(s) 694, 1220
TaaI ACNGT 3 cut(s) 1055, 1064, 1537
TaiI ACGT 3 cut(s) 423, 1284, 1517
TaqI TCGA 9 cut(s) 150, 276, 333, 435, 472, 805, 943, 1123, 1446
TaqII GACCGA 1 cut(s) 21
TasI AATT 4 cut(s) 452, 634, 857, 1307
TatI WGTACW 2 cut(s) 773, 1374
TauI GCSGC 2 cut(s) 176, 239
TfiI GAWTC 8 cut(s) 41, 225, 293, 335, 373, 555, 651, 755
Tru1I TTAA 3 cut(s) 1116, 1152, 1431
Tru9I TTAA 3 cut(s) 1116, 1152, 1431
TscAI CASTG 4 cut(s) 122, 828, 1069, 1512
TseFI GTSAC 3 cut(s) 88, 131, 1493
TseI GCWGC 6 cut(s) 231, 460, 590, 627, 677, 1244
Tsp45I GTSAC 3 cut(s) 88, 131, 1493
TspGWI ACGGA 5 cut(s) 120, 311, 366, 982, 1050
TspRI CASTG 4 cut(s) 122, 828, 1069, 1512
VpaK11BI GGWCC 5 cut(s) 4, 160, 1409, 1423, 1462
XapI RAATTY 1 cut(s) 857
XceI RCATGY 2 cut(s) 488, 1092
XspI CTAG 4 cut(s) 509, 947, 1047, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.