Rorug04G0422500

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008029
Physical Location & Seq
Reverse (-)
58210354 .. 58211175
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0422500.1

Sequence Viewer

Length: 822 bp
ATGGCTGGAGAAATTCCTGCGAAGAAGCTTATCTTGTGTGGTGGTGATGATGATGGTGATGATGACAAATATTACGAAGACACGAGAAGTGAATGTAGCCTTTCACTAGATCGGCTGACTTTAGAACCCAGAAGAAAGAAGCTTCTTGTTTTTGATCTGAATGGGTTACTAGTCTACAGGGTTTATCGTTCCAACAAGCCTGAATTTCCAAGTGATCGTACACCGGATGGGAGATTTGGAAACCATCTAGTTTTCAAAAGACCTTTTGCTGAGGAGTTTGTGCAATTCTGCCTTGAAAGATTCGAGATCGGAATATGGTCTTCTGCCCTAGAGAAAAATGTTGATGCCGTCTTAGATTGTATGATGGCAAATCAGAGAAATAGACTATTATTTGTGTGGGGTCAATCTCAATGTACAGATTCTGGGTTCAAGTCCTTGGAGAAAAGAATGAAGCCTCTATTTTTCAAAGAACTGAAGAAAGTGTGGTATCATTTCGGCGGAAAGTATTCTGAATGTGATACTTTGTTGATTGATGATCAACCATACAAGGCCCTACTCAATCCTGCACACACAGGCATTTTTCTGGAATCCTACAATCCTGACAATGCTAGTGATAATTTATTGGATCCAAATGGACAGCTAGGGGTGTATTTGGATGGCCTTGCGGCTGCGACTAATGCTCAGGTTTATGTAAAGGAGAACCCTATTGGAGTGGCTGCAATATCATCAGATCATCCTGATTGGAATTTCTACTCTGAAGTCCTTCGAGGGATTAAGGGAGAAAGTGAGGAAAAGATTACGCCTCAGCAACAAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

273

Amino Acids

31.1

Weight (kDa)

5.03

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 47 - 218 8.4e-21 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AciI CCGC 2 cut(s) 498, 665
AclWI GGATC 2 cut(s) 620, 633
AcsI RAATTY 3 cut(s) 12, 203, 745
AcuI CTGAAG 2 cut(s) 494, 777
AfaI GTAC 2 cut(s) 220, 415
AgsI TTSAA 4 cut(s) 256, 296, 430, 466
AhlI ACTAGT 1 cut(s) 169
AluBI AGCT 3 cut(s) 28, 142, 640
AluI AGCT 3 cut(s) 28, 142, 640
AlwI GGATC 2 cut(s) 620, 633
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 2 cut(s) 549, 658
ApeKI GCWGC 2 cut(s) 668, 716
ApoI RAATTY 3 cut(s) 12, 203, 745
Asp700I GAANNNNTTC 2 cut(s) 505, 762
AspS9I GGNCC 1 cut(s) 550
AsuHPI GGTGA 2 cut(s) 56, 68
BamHI GGATCC 1 cut(s) 625
BauI CACGAG 1 cut(s) 82
BbsI GAAGAC 2 cut(s) 84, 312
BbvCI CCTCAGC 2 cut(s) 270, 804
BbvI GCAGC 2 cut(s) 655, 703
BccI CCATC 5 cut(s) 47, 221, 252, 358, 650
BceAI ACGGC 1 cut(s) 332
BclI TGATCA 1 cut(s) 535
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 6 cut(s) 107, 170, 248, 329, 609, 641
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 3 cut(s) 666, 669, 717
BlsI GCNGC 3 cut(s) 667, 670, 718
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 1 cut(s) 627
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 2 cut(s) 84, 312
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 3 cut(s) 270, 681, 804
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 3 cut(s) 232, 661, 733
BseMII CTCAG 3 cut(s) 261, 695, 818
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 2 cut(s) 655, 703
BsgI GTGCAG 1 cut(s) 549
BshFI GGCC 2 cut(s) 551, 660
BsiSI CCGG 1 cut(s) 224
BsnI GGCC 2 cut(s) 551, 660
Bsp1407I TGTACA 1 cut(s) 413
Bsp143I GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BspACI CCGC 2 cut(s) 498, 665
BspANI GGCC 2 cut(s) 551, 660
BspCNI CTCAG 3 cut(s) 262, 694, 817
BspLI GGNNCC 1 cut(s) 627
BspPI GGATC 2 cut(s) 620, 633
BsrGI TGTACA 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BssSI CACGAG 1 cut(s) 82
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 82
BstAUI TGTACA 1 cut(s) 413
BstDEI CTNAG 4 cut(s) 270, 352, 681, 804
BstF5I GGATG 3 cut(s) 232, 661, 733
BstKTI GATC 7 cut(s) 112, 157, 217, 309, 538, 628, 733
BstMBI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 655, 703
BstV2I GAAGAC 2 cut(s) 84, 312
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 2 cut(s) 551, 660
BtsCI GGATG 3 cut(s) 232, 661, 733
CaiI CAGNNNCTG 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 550
Csp6I GTAC 2 cut(s) 219, 414
CviQI GTAC 2 cut(s) 219, 414
DdeI CTNAG 4 cut(s) 270, 352, 681, 804
DpnI GATC 7 cut(s) 111, 156, 216, 308, 537, 627, 732
DpnII GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
EciI GGCGGA 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 435
Eco57I CTGAAG 2 cut(s) 494, 777
EcoO109I RGGNCCY 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaiI YATR 4 cut(s) 316, 362, 544, 690
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 666, 669, 717
FokI GGATG 3 cut(s) 239, 668, 720
Fsp4HI GCNGC 3 cut(s) 666, 669, 717
FspBI CTAG 6 cut(s) 107, 170, 248, 329, 609, 641
GluI GCNGC 3 cut(s) 666, 669, 717
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 2 cut(s) 551, 660
HapII CCGG 1 cut(s) 224
HindIII AAGCTT 2 cut(s) 26, 140
HinfI GANTC 4 cut(s) 300, 419, 587, 815
HpaII CCGG 1 cut(s) 224
HphI GGTGA 2 cut(s) 56, 68
Hpy166II GTNNAC 2 cut(s) 175, 221
Hpy188I TCNGA 6 cut(s) 159, 311, 375, 511, 730, 757
Hpy188III TCNNGA 5 cut(s) 304, 584, 599, 737, 819
Hpy8I GTNNAC 2 cut(s) 175, 221
HpyAV CCTTC 1 cut(s) 773
HpyCH4V TGCA 3 cut(s) 283, 566, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 4 cut(s) 270, 352, 681, 804
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
Lsp1109I GCAGC 2 cut(s) 655, 703
LweI GCATC 1 cut(s) 334
MaeI CTAG 6 cut(s) 107, 170, 248, 329, 609, 641
MaeIII GTNAC 1 cut(s) 165
MalI GATC 7 cut(s) 111, 156, 216, 308, 537, 627, 732
MboI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
MboII GAAGA 5 cut(s) 34, 89, 144, 312, 487
MflI RGATCY 1 cut(s) 625
MluCI AATT 5 cut(s) 12, 203, 284, 616, 745
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 5 cut(s) 265, 465, 761, 781, 813
MroXI GAANNNNTTC 2 cut(s) 505, 762
MseI TTAA 1 cut(s) 774
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeII GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
NlaIV GGNNCC 1 cut(s) 627
PdmI GAANNNNTTC 2 cut(s) 505, 762
PfeI GAWTC 3 cut(s) 300, 419, 587
PkrI GCNGC 3 cut(s) 667, 670, 718
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 625
RsaI GTAC 2 cut(s) 220, 415
RsaNI GTAC 2 cut(s) 219, 414
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 3 cut(s) 666, 669, 717
Sau3AI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
Sau96I GGNCC 1 cut(s) 550
SetI ASST 5 cut(s) 30, 144, 265, 642, 687
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 175
SpeI ACTAGT 1 cut(s) 169
Sse9I AATT 5 cut(s) 12, 203, 284, 616, 745
SsiI CCGC 2 cut(s) 498, 665
SspI AATATT 1 cut(s) 71
SspMI CTAG 6 cut(s) 107, 170, 248, 329, 609, 641
StyI CCWWGG 1 cut(s) 435
TaqI TCGA 2 cut(s) 303, 766
TasI AATT 5 cut(s) 12, 203, 284, 616, 745
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 3 cut(s) 300, 419, 587
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TseI GCWGC 2 cut(s) 668, 716
TspDTI ATGAA 1 cut(s) 464
XapI RAATTY 3 cut(s) 12, 203, 745
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 505, 762
XspI CTAG 6 cut(s) 107, 170, 248, 329, 609, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.