pycom10g25510

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008029
Physical Location & Seq
Reverse (-)
27130559 .. 27131545
987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25510.2

Sequence Viewer

Length: 492 bp
ATGCTGTTCTCGCCATTATTCGAGCGTTCACTGGATGTAGCTACCATATGCCAGGGGAAGACCAAATTCTTTAGTGTATTGATGCTTGCATGGGGTTTAGTGGCAGATGTTGACATTGAGTCTGAGAAGTATAGGTGGATGGGAAGTGCTCGACTAGATTTCTATGCTGTTCAACGGATTGTGCATTTGAGGCAATATAATGGATATATTTCCTTTGTGCCTGCACCGGGCTTTGAAACTTACGGAGAGCCTACTAGTTACAGTAATGACGGTGAACCTGCTAACCAACACAATGCATGTGCTCCGAATCACGAGGAGCCGATTAACATTCGACAGCATGGCTATGAGGGGCCTGATATTAACTTGCATAGCATGGACTGGAGAACCATTAGGGGGCCCTTCGTTTCAGTCTGGCTTCACAATGTGCCTTGGGGTGGTGAAGGCACAAAGGCAGCGCCTGATGCCAAGTTCTCGGATGGTTATATTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

164

Amino Acids

18.44

Weight (kDa)

5.43

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AcsI RAATTY 1 cut(s) 65
AdeI CACNNNGTG 1 cut(s) 424
AfiI CCNNNNNNNGG 3 cut(s) 227, 393, 434
AgsI TTSAA 2 cut(s) 173, 236
AhdI GACNNNNNGTC 1 cut(s) 118
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
Alw21I GWGCWC 2 cut(s) 151, 304
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 2 cut(s) 350, 395
ApaI GGGCCC 1 cut(s) 399
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 1 cut(s) 65
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 3 cut(s) 350, 395, 396
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 284, 449
BaeGI GKGCMC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 399
BauI CACGAG 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 2 cut(s) 151, 304
BbvI GCAGC 1 cut(s) 464
BccI CCATC 2 cut(s) 133, 470
BciT130I CCWGG 1 cut(s) 53
BcnI CCSGG 1 cut(s) 228
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 2 cut(s) 155, 255
BfoI RGCGCY 1 cut(s) 458
BfuAI ACCTGC 1 cut(s) 286
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 2 cut(s) 53, 228
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 3 cut(s) 350, 395, 396
BmiI GGNNCC 4 cut(s) 318, 351, 396, 397
BmrFI CCNGG 2 cut(s) 53, 228
BmsI GCATC 2 cut(s) 72, 451
BpiI GAAGAC 1 cut(s) 65
BpmI CTGGAG 1 cut(s) 400
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 52, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 393, 434
Bse1I ACTGG 2 cut(s) 36, 383
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 52, 428
BseGI GGATG 3 cut(s) 40, 144, 481
BseLI CCNNNNNNNGG 3 cut(s) 227, 393, 434
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 36, 383
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 399
BseXI GCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 2 cut(s) 352, 397
BsiHKAI GWGCWC 2 cut(s) 151, 304
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 3 cut(s) 227, 393, 434
BsnI GGCC 2 cut(s) 352, 397
Bsp120I GGGCCC 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 151, 304, 399
BspANI GGCC 2 cut(s) 352, 397
BspCNI CTCAG 1 cut(s) 115
BspLI GGNNCC 4 cut(s) 318, 351, 396, 397
BspMI ACCTGC 1 cut(s) 286
BsrI ACTGG 2 cut(s) 36, 383
BssECI CCNNGG 2 cut(s) 52, 428
BssSI CACGAG 1 cut(s) 311
BssT1I CCWWGG 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 311
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 2 cut(s) 263, 272
BstC8I GCNNGC 2 cut(s) 87, 222
BstDEI CTNAG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 40, 144, 481
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 1 cut(s) 457
BstMWI GCNNNNNNNGC 3 cut(s) 10, 190, 461
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 1 cut(s) 300
BstSCI CCNGG 2 cut(s) 51, 226
BstSLI GKGCMC 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 1 cut(s) 65
BsuRI GGCC 2 cut(s) 352, 397
BtsCI GGATG 3 cut(s) 40, 144, 481
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 87, 222
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 3 cut(s) 350, 395, 396
CviAII CATG 4 cut(s) 90, 297, 338, 373
CviJI RGCY 8 cut(s) 41, 231, 250, 319, 342, 352, 397, 415
CviKI_1 RGCY 8 cut(s) 41, 231, 250, 319, 342, 352, 397, 415
DdeI CTNAG 1 cut(s) 123
DraIII CACNNNGTG 1 cut(s) 424
DriI GACNNNNNGTC 1 cut(s) 118
Eam1105I GACNNNNNGTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 399
EcoO109I RGGNCCY 3 cut(s) 350, 395, 396
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 428
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 428
FaeI CATG 4 cut(s) 93, 300, 341, 376
FatI CATG 4 cut(s) 89, 296, 337, 372
FauNDI CATATG 1 cut(s) 47
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 3 cut(s) 47, 151, 488
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 2 cut(s) 155, 255
GlaI GCGC 1 cut(s) 456
GluI GCNGC 1 cut(s) 453
GsuI CTGGAG 1 cut(s) 400
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 2 cut(s) 352, 397
HapII CCGG 1 cut(s) 227
HhaI GCGC 1 cut(s) 457
Hin1II CATG 4 cut(s) 93, 300, 341, 376
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 2 cut(s) 119, 307
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 284, 449
Hpy166II GTNNAC 3 cut(s) 29, 112, 275
Hpy188I TCNGA 3 cut(s) 124, 306, 475
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 3 cut(s) 29, 112, 275
HpyAV CCTTC 2 cut(s) 409, 434
HpyCH4III ACNGT 2 cut(s) 263, 272
HpyCH4V TGCA 5 cut(s) 89, 184, 224, 296, 367
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 190, 461
HpyF3I CTNAG 1 cut(s) 123
Hsp92II CATG 4 cut(s) 93, 300, 341, 376
HspAI GCGC 1 cut(s) 455
LmnI GCTCC 2 cut(s) 307, 316
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 2 cut(s) 72, 451
MaeI CTAG 2 cut(s) 155, 255
MaeIII GTNAC 1 cut(s) 257
MboII GAAGA 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 151, 304, 399
MluCI AATT 1 cut(s) 65
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 3 cut(s) 183, 307, 340
Mph1103I ATGCAT 1 cut(s) 298
MseI TTAA 2 cut(s) 324, 360
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 53, 228
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 10, 190, 461
NciI CCSGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 47
NlaIII CATG 4 cut(s) 93, 300, 341, 376
NlaIV GGNNCC 4 cut(s) 318, 351, 396, 397
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 1 cut(s) 300
PfeI GAWTC 1 cut(s) 307
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 4 cut(s) 318, 351, 396, 397
PspOMI GGGCCC 1 cut(s) 395
PspPI GGNCC 3 cut(s) 350, 395, 396
PstNI CAGNNNCTG 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 2 cut(s) 324, 360
SatI GCNGC 1 cut(s) 453
Sau96I GGNCC 3 cut(s) 350, 395, 396
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 53, 228
SduI GDGCHC 3 cut(s) 151, 304, 399
SetI ASST 3 cut(s) 43, 137, 280
SfaNI GCATC 2 cut(s) 72, 451
SmiMI CAYNNNNRTG 1 cut(s) 342
SpeI ACTAGT 1 cut(s) 254
Sse9I AATT 1 cut(s) 65
SspMI CTAG 2 cut(s) 155, 255
StyD4I CCNGG 2 cut(s) 51, 226
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 2 cut(s) 263, 272
TaqI TCGA 3 cut(s) 21, 151, 331
TasI AATT 1 cut(s) 65
TfiI GAWTC 1 cut(s) 307
Tru1I TTAA 2 cut(s) 324, 360
Tru9I TTAA 2 cut(s) 324, 360
TscAI CASTG 1 cut(s) 36
TseI GCWGC 1 cut(s) 452
TspGWI ACGGA 2 cut(s) 190, 258
TspRI CASTG 1 cut(s) 36
XapI RAATTY 1 cut(s) 65
XceI RCATGY 1 cut(s) 300
XspI CTAG 2 cut(s) 155, 255
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.