FvH4_3g04681

Polyphenol oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005180
Physical Location & Seq
Reverse (-)
2690932 .. 2692416
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04681.t1

Sequence Viewer

Length: 567 bp
ATGAGTGAAACTCCCCCGGCGTTCGGTGAATCCGAAGCATATGCTGCAGCCGAGTCAACGTCCAAGAAGGTAGTAGAACTCACTGAGTTTCCGATCACTATGGACTCGGTGATCAGCGTCACGGTGCCGAGGCCGAAGAAGTGTCGGACAAAGGAGGAGAAGGAAGAGGAAGAGGAGGTGTTGGCGATTCAAGGGATAGAGTTTGTGGCGGATGAGCTTGTGAAGTTCGACGTGCATGTCAACGATGACGAAGACGATCTGAGTCGACCCGACAACTCGGAGTTCGCCGGAAGCTTTGTGTATTTGCCTCATCGGAGGAAGACGGTGACGACGAGCCTGAGGCTTGGGATAACGGACTTGTTGGATGATTTGGGTGCTGATGAAGATGACAGTATCAAGGTGACTTTAGTGCCAAAGTGTGTGAAACGACCTGTCACTATTGGGAAGCTCAAGATCGAGTTTCTCAAATACTTTTGGCGCAAATATAGTTGGGAGTGGGGGAGCTGGTGTGATGCATATGTAACATGTTTTCCTTGGCATAAGCCATATAAGGTTAAGGCCAGCTAG

Protein Analysis

189

Amino Acids

21.5

Weight (kDa)

4.97

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPO1_KFDV PF12143 30 - 154 1.7e-46 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 265
AciI CCGC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 524
AgsI TTSAA 1 cut(s) 191
AjiI CACGTC 1 cut(s) 232
AluBI AGCT 5 cut(s) 217, 294, 448, 504, 564
AluI AGCT 5 cut(s) 217, 294, 448, 504, 564
AoxI GGCC 2 cut(s) 131, 558
ApeKI GCWGC 2 cut(s) 44, 47
AspLEI GCGC 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 4 cut(s) 38, 121, 337, 412
AxyI CCTNAGG 1 cut(s) 338
BanI GGYRCC 1 cut(s) 124
BbsI GAAGAC 2 cut(s) 258, 326
BbvI GCAGC 2 cut(s) 31, 59
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BclI TGATCA 1 cut(s) 111
BcnI CCSGG 1 cut(s) 17
BfaI CTAG 1 cut(s) 565
BfmI CTRYAG 1 cut(s) 45
BisI GCNGC 2 cut(s) 45, 48
BlsI GCNGC 2 cut(s) 46, 49
Bme1390I CCNGG 1 cut(s) 17
BmgBI CACGTC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 126
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 502
BpiI GAAGAC 2 cut(s) 258, 326
BplI GAGNNNNNCTC 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 15, 128, 533
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse21I CCTNAGG 1 cut(s) 338
BseDI CCNNGG 3 cut(s) 15, 128, 533
BseGI GGATG 2 cut(s) 217, 370
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMII CTCAG 3 cut(s) 75, 251, 329
BseRI GAGGAG 2 cut(s) 170, 188
BseXI GCAGC 2 cut(s) 31, 59
BshFI GGCC 2 cut(s) 133, 560
BshNI GGYRCC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 17, 288
BslI CCNNNNNNNGG 1 cut(s) 23
BsnI GGCC 2 cut(s) 133, 560
Bsp143I GATC 4 cut(s) 93, 111, 256, 453
BspACI CCGC 1 cut(s) 209
BspANI GGCC 2 cut(s) 133, 560
BspCNI CTCAG 3 cut(s) 76, 252, 330
BspLI GGNNCC 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 15, 128, 533
BssMI GATC 4 cut(s) 93, 111, 256, 453
BssT1I CCWWGG 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 124, 325, 392
Bst6I CTCTTC 2 cut(s) 159, 165
BstAPI GCANNNNNTGC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 3 cut(s) 84, 260, 338
BstF5I GGATG 2 cut(s) 217, 370
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 4 cut(s) 96, 114, 259, 456
BstMBI GATC 4 cut(s) 93, 111, 256, 453
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNSI RCATGY 2 cut(s) 239, 528
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 31, 59
BstV2I GAAGAC 2 cut(s) 258, 326
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 2 cut(s) 133, 560
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 2 cut(s) 217, 370
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 562
CfoI GCGC 1 cut(s) 480
CseI GACGC 1 cut(s) 106
CviAII CATG 2 cut(s) 236, 525
DdeI CTNAG 3 cut(s) 84, 260, 338
DpnI GATC 4 cut(s) 95, 113, 258, 455
DpnII GATC 4 cut(s) 93, 111, 256, 453
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 2 cut(s) 159, 165
EarI CTCTTC 2 cut(s) 159, 165
EciI GGCGGA 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 533
Eco81I CCTNAGG 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 533
EcoT22I ATGCAT 1 cut(s) 517
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 239, 528
FatI CATG 2 cut(s) 235, 524
FauNDI CATATG 2 cut(s) 40, 517
FbaI TGATCA 1 cut(s) 111
FblI GTMKAC 1 cut(s) 265
Fnu4HI GCNGC 2 cut(s) 45, 48
FokI GGATG 2 cut(s) 224, 377
Fsp4HI GCNGC 2 cut(s) 45, 48
FspBI CTAG 1 cut(s) 565
GlaI GCGC 1 cut(s) 479
GluI GCNGC 2 cut(s) 45, 48
HaeIII GGCC 2 cut(s) 133, 560
HapII CCGG 2 cut(s) 17, 288
HgaI GACGC 1 cut(s) 106
HhaI GCGC 1 cut(s) 480
Hin1II CATG 2 cut(s) 239, 528
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HincII GTYRAC 3 cut(s) 57, 241, 266
HindII GTYRAC 3 cut(s) 57, 241, 266
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 5 cut(s) 29, 53, 104, 187, 262
HpaII CCGG 2 cut(s) 17, 288
HphI GGTGA 4 cut(s) 38, 121, 337, 412
Hpy166II GTNNAC 3 cut(s) 57, 241, 266
Hpy188I TCNGA 6 cut(s) 34, 93, 147, 261, 280, 315
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 3 cut(s) 57, 241, 266
Hpy99I CGWCG 2 cut(s) 233, 334
HpyAV CCTTC 2 cut(s) 61, 154
HpyCH4III ACNGT 3 cut(s) 124, 325, 392
HpyCH4IV ACGT 2 cut(s) 59, 231
HpyCH4V TGCA 3 cut(s) 47, 235, 515
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
HpyF3I CTNAG 3 cut(s) 84, 260, 338
HpySE526I ACGT 2 cut(s) 59, 231
Hsp92II CATG 2 cut(s) 239, 528
HspAI GCGC 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 4 cut(s) 93, 111, 256, 453
LmnI GCTCC 1 cut(s) 501
LpnPI CCDG 5 cut(s) 30, 301, 350, 444, 490
Lsp1109I GCAGC 2 cut(s) 31, 59
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 565
MaeII ACGT 2 cut(s) 59, 231
MaeIII GTNAC 5 cut(s) 118, 325, 400, 433, 520
MalI GATC 4 cut(s) 95, 113, 258, 455
MboI GATC 4 cut(s) 93, 111, 256, 453
MboII GAAGA 6 cut(s) 148, 176, 182, 263, 331, 395
MlyI GAGTC 3 cut(s) 62, 98, 271
MmeI TCCRAC 2 cut(s) 125, 342
MnlI CCTC 8 cut(s) 123, 148, 160, 166, 169, 309, 318, 333
Mph1103I ATGCAT 1 cut(s) 517
MseI TTAA 1 cut(s) 555
MspI CCGG 2 cut(s) 17, 288
MspR9I CCNGG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 44
NciI CCSGG 1 cut(s) 17
NdeI CATATG 2 cut(s) 40, 517
NdeII GATC 4 cut(s) 93, 111, 256, 453
NlaIII CATG 2 cut(s) 239, 528
NlaIV GGNNCC 1 cut(s) 126
NmeAIII GCCGAG 2 cut(s) 76, 153
NmuCI GTSAC 4 cut(s) 118, 325, 400, 433
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 2 cut(s) 239, 528
PciI ACATGT 1 cut(s) 524
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 329
PfeI GAWTC 2 cut(s) 29, 187
PkrI GCNGC 2 cut(s) 46, 49
PleI GAGTC 3 cut(s) 61, 98, 270
PpsI GAGTC 3 cut(s) 61, 98, 270
PscI ACATGT 1 cut(s) 524
PspN4I GGNNCC 1 cut(s) 126
PstI CTGCAG 1 cut(s) 49
SalI GTCGAC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 555
SatI GCNGC 2 cut(s) 45, 48
Sau3AI GATC 4 cut(s) 93, 111, 256, 453
SchI GAGTC 3 cut(s) 62, 98, 271
ScrFI CCNGG 1 cut(s) 17
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SsiI CCGC 1 cut(s) 209
SspMI CTAG 1 cut(s) 565
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 124, 325, 392
TaiI ACGT 2 cut(s) 62, 234
TaqI TCGA 3 cut(s) 228, 265, 456
TfiI GAWTC 2 cut(s) 29, 187
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 4 cut(s) 118, 325, 400, 433
TseI GCWGC 2 cut(s) 44, 47
Tsp45I GTSAC 4 cut(s) 118, 325, 400, 433
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 1 cut(s) 368
TspRI CASTG 1 cut(s) 88
XceI RCATGY 2 cut(s) 239, 528
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 1 cut(s) 565
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.