MD10G1298200.v1.1

Polyphenol oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005180
Physical Location & Seq
Forward (+)
38528342 .. 38529851
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1298200.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGCTTCTTCTCTTCCATTATCATCCAACACCAAGATTGCCACTTACGCTATCGCCCCTTCACTCCCTCAAATCTTCCCAAACAAATCCCAGATCTCCTTAGTCAGAAATCTTAGAGGTTCATGTAAAGCAACAACAATCAACAGTGACCAAAACCCTAATCATGATCCGCAAACTTGTCTGGTAAAATTGGATAGGAGAAATCTCCTCCTTGGTCTAGGAGGACTTGCCTTGGCTACTCATAACATAACAAGGCCAGCAAACGCGGCAGAGACGTCAAAGAAGGTAGTTTTGGTGGAGAGTAGTGATTTGCCAATAGCTTTGGACTCTGTGGTGAGCGTGATCGTGCCGCGGCCGAGGAGGTCGAGGAGCAAGAAGGACAAGGAGGAGGAAGAGGAGGTGTTGGCGATTGAAGGGATTGAGTTTGTGGCAGACAAGGTGCTCAAGTTCGACGTGCATGTGAACGATGATGAGGACTCGCTGTGTCGACCTGACAAGTCTGAGTTTGCAGGGAGCTTGGTGTTTTTGCCACAGTCCAAGAAAAAGGTTAAAACCAGCCTGCATCTTGGGATTTCGGCCTTGTTGGAGGACTTGGGAGTTGACGATGATGGCAGTATTAAGGTAACTTTGGTGCCAAGGAATGTCCAGCGACCCGTCACCATTGGTCGGATCAAGATCGACTATTTTGCCTAG

Protein Analysis

231

Amino Acids

25.1

Weight (kDa)

8.38

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPO1_KFDV PF12143 105 - 228 1.6e-42 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 487
AccII CGCG 2 cut(s) 266, 352
AciI CCGC 4 cut(s) 170, 266, 350, 352
AclWI GGATC 2 cut(s) 161, 677
AcoI YGGCCR 1 cut(s) 353
AcyI GRCGYC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 666
AgsI TTSAA 1 cut(s) 413
AjiI CACGTC 1 cut(s) 454
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 2 cut(s) 320, 516
AluI AGCT 2 cut(s) 320, 516
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 2 cut(s) 161, 677
AoxI GGCC 3 cut(s) 254, 353, 576
AsuHPI GGTGA 2 cut(s) 346, 649
BanI GGYRCC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 444
BccI CCATC 1 cut(s) 602
BcgI CGANNNNNNTGC 1 cut(s) 668
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 2 cut(s) 218, 691
BglII AGATCT 1 cut(s) 93
BisI GCNGC 3 cut(s) 267, 350, 353
BlsI GCNGC 3 cut(s) 268, 351, 354
BmgBI CACGTC 1 cut(s) 454
BmiI GGNNCC 1 cut(s) 633
BmsI GCATC 1 cut(s) 571
BpuEI CTTGAG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 275
BsaJI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
Bsc4I CCNNNNNNNGG 1 cut(s) 666
Bse8I GATNNNNATC 1 cut(s) 674
BseDI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
BseGI GGATG 1 cut(s) 23
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 1 cut(s) 666
BseMII CTCAG 1 cut(s) 492
BseRI GAGGAG 5 cut(s) 197, 373, 382, 401, 410
BseX3I CGGCCG 1 cut(s) 353
Bsh1236I CGCG 2 cut(s) 266, 352
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 256, 355, 578
BshNI GGYRCC 1 cut(s) 631
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 444
BslI CCNNNNNNNGG 1 cut(s) 666
BsmAI GTCTC 1 cut(s) 266
BsmBI CGTCTC 1 cut(s) 266
BsnI GGCC 3 cut(s) 256, 355, 578
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 5 cut(s) 93, 166, 342, 669, 675
BspACI CCGC 4 cut(s) 170, 266, 350, 352
BspANI GGCC 3 cut(s) 256, 355, 578
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 2 cut(s) 266, 352
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 633
BspPI GGATC 2 cut(s) 161, 677
BspT107I GGYRCC 1 cut(s) 631
BssECI CCNNGG 5 cut(s) 211, 231, 350, 356, 635
BssMI GATC 5 cut(s) 93, 166, 342, 669, 675
BssNI GRCGYC 1 cut(s) 275
BssT1I CCWWGG 3 cut(s) 211, 231, 635
Bst4CI ACNGT 2 cut(s) 146, 534
Bst6I CTCTTC 2 cut(s) 18, 387
BstACI GRCGYC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 258, 560
BstDEI CTNAG 3 cut(s) 100, 113, 501
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 1 cut(s) 23
BstFNI CGCG 2 cut(s) 266, 352
BstKTI GATC 5 cut(s) 96, 169, 345, 672, 678
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 5 cut(s) 93, 166, 342, 669, 675
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 47, 266
BstNSI RCATGY 1 cut(s) 461
BstUI CGCG 2 cut(s) 266, 352
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BstZI CGGCCG 1 cut(s) 353
BsuRI GGCC 3 cut(s) 256, 355, 578
BtgI CCRYGG 1 cut(s) 350
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 151
Cac8I GCNNGC 2 cut(s) 258, 560
CciI TCATGA 1 cut(s) 163
Cfr42I CCGCGG 1 cut(s) 353
CviAII CATG 3 cut(s) 123, 164, 458
CviJI RGCY 8 cut(s) 5, 236, 256, 320, 355, 516, 558, 578
CviKI_1 RGCY 8 cut(s) 5, 236, 256, 320, 355, 516, 558, 578
DdeI CTNAG 3 cut(s) 100, 113, 501
DpnI GATC 5 cut(s) 95, 168, 344, 671, 677
DpnII GATC 5 cut(s) 93, 166, 342, 669, 675
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 18, 387
EarI CTCTTC 2 cut(s) 18, 387
EclXI CGGCCG 1 cut(s) 353
Eco130I CCWWGG 3 cut(s) 211, 231, 635
Eco52I CGGCCG 1 cut(s) 353
EcoT14I CCWWGG 3 cut(s) 211, 231, 635
ErhI CCWWGG 3 cut(s) 211, 231, 635
Esp3I CGTCTC 1 cut(s) 266
FaeI CATG 3 cut(s) 126, 167, 461
FaiI YATR 5 cut(s) 124, 165, 243, 248, 459
FatI CATG 3 cut(s) 122, 163, 457
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 3 cut(s) 267, 350, 353
FokI GGATG 1 cut(s) 10
Fsp4HI GCNGC 3 cut(s) 267, 350, 353
FspBI CTAG 2 cut(s) 218, 691
GluI GCNGC 3 cut(s) 267, 350, 353
HaeIII GGCC 3 cut(s) 256, 355, 578
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 3 cut(s) 126, 167, 461
HincII GTYRAC 2 cut(s) 488, 601
HindII GTYRAC 2 cut(s) 488, 601
HinfI GANTC 2 cut(s) 326, 476
HphI GGTGA 2 cut(s) 346, 649
Hpy166II GTNNAC 3 cut(s) 463, 488, 601
Hpy188I TCNGA 3 cut(s) 107, 502, 669
Hpy188III TCNNGA 2 cut(s) 164, 673
Hpy8I GTNNAC 3 cut(s) 463, 488, 601
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 4 cut(s) 69, 277, 370, 407
HpyCH4III ACNGT 2 cut(s) 146, 534
HpyCH4IV ACGT 2 cut(s) 275, 453
HpyCH4V TGCA 3 cut(s) 457, 509, 562
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 266
HpyF3I CTNAG 3 cut(s) 100, 113, 501
HpySE526I ACGT 2 cut(s) 275, 453
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 3 cut(s) 126, 167, 461
KspI CCGCGG 1 cut(s) 353
Kzo9I GATC 5 cut(s) 93, 166, 342, 669, 675
LmnI GCTCC 2 cut(s) 369, 513
LpnPI CCDG 8 cut(s) 104, 167, 270, 495, 504, 568, 572, 659
LweI GCATC 1 cut(s) 571
MaeI CTAG 2 cut(s) 218, 691
MaeII ACGT 2 cut(s) 275, 453
MaeIII GTNAC 3 cut(s) 146, 622, 655
MalI GATC 5 cut(s) 95, 168, 344, 671, 677
MboI GATC 5 cut(s) 93, 166, 342, 669, 675
MboII GAAGA 3 cut(s) 5, 67, 404
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 1 cut(s) 188
MlyI GAGTC 2 cut(s) 320, 470
MmeI TCCRAC 3 cut(s) 51, 564, 647
MseI TTAA 2 cut(s) 549, 618
MspA1I CMGCKG 1 cut(s) 352
MvnI CGCG 2 cut(s) 266, 352
MwoI GCNNNNNNNGC 2 cut(s) 47, 266
NdeII GATC 5 cut(s) 93, 166, 342, 669, 675
NlaIII CATG 3 cut(s) 126, 167, 461
NlaIV GGNNCC 1 cut(s) 633
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 2 cut(s) 146, 655
NspI RCATGY 1 cut(s) 461
PagI TCATGA 1 cut(s) 163
PkrI GCNGC 3 cut(s) 268, 351, 354
PleI GAGTC 2 cut(s) 320, 470
PpsI GAGTC 2 cut(s) 320, 470
PspN4I GGNNCC 1 cut(s) 633
PsuI RGATCY 1 cut(s) 93
SacII CCGCGG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 486
SaqAI TTAA 2 cut(s) 549, 618
SatI GCNGC 3 cut(s) 267, 350, 353
Sau3AI GATC 5 cut(s) 93, 166, 342, 669, 675
SchI GAGTC 2 cut(s) 320, 470
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 1 cut(s) 571
Sfr303I CCGCGG 1 cut(s) 353
SgrBI CCGCGG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 1 cut(s) 188
SsiI CCGC 4 cut(s) 170, 266, 350, 352
SspMI CTAG 2 cut(s) 218, 691
StyI CCWWGG 3 cut(s) 211, 231, 635
TaaI ACNGT 2 cut(s) 146, 534
TaiI ACGT 2 cut(s) 278, 456
TaqI TCGA 4 cut(s) 365, 450, 487, 678
TasI AATT 1 cut(s) 188
TauI GCSGC 3 cut(s) 269, 352, 355
Tru1I TTAA 2 cut(s) 549, 618
Tru9I TTAA 2 cut(s) 549, 618
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 2 cut(s) 146, 655
Tsp45I GTSAC 2 cut(s) 146, 655
TspDTI ATGAA 1 cut(s) 111
TspRI CASTG 1 cut(s) 151
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 487
XspI CTAG 2 cut(s) 218, 691
ZraI GACGTC 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.