FvH4_3g06280

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008972
Physical Location & Seq
Forward (+)
3611147 .. 3614162
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06280.t1

Sequence Viewer

Length: 534 bp
ATGGAGTCATGCCCTACTGTAAAGAACATCCTCCTCCTAGATTCTGAAGGAAAACGTGTAGCTGTCAAGTATTATTCAGAGGACTGGCCAACGAATAATGCAAAAGAAGCTTTTGAGAAAGCTGTTTTCACTAAAACTCAAAAGACAAATGCACGGACAGAAGCTGAGATAGCGATGTTTGAGAACAGCATAGTAGTTTACAAGTTTGTTCAAGACCTTCACTTTTTTGTTACTGGGGGTGATAATGAAAATGAGCTCATTTTAGCCACAGTTCTTCAGGGATTTTTTGATGCAGTTGGAATTCTCCTCAGAGGCAATGTGGACAAAAAGGAAGCACTTGAGAATTTGGACCTCATTCTACTATGCCTTGATGAAATTGTTGATGGCGGCATTGTTCTTGAGACCGATTCAAATGTTATAGCAAGTAAGGTTGCGAGTCATAGTATTGATTCTGGAGCTCCATTATCTGAGCAGACAATAGGCCAAGCACTTGCTACTGCACGCGAACATTTTGCCAGATCTCTTCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

178

Amino Acids

19.47

Weight (kDa)

4.75

Isoelectric Point (pI)

32.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 6 - 146 5.6e-24 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 504
AciI CCGC 1 cut(s) 387
AcoI YGGCCR 1 cut(s) 86
AcsI RAATTY 2 cut(s) 300, 343
AcuI CTGAAG 2 cut(s) 66, 260
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 2 cut(s) 212, 411
AluBI AGCT 6 cut(s) 62, 110, 122, 164, 256, 458
AluI AGCT 6 cut(s) 62, 110, 122, 164, 256, 458
Alw21I GWGCWC 2 cut(s) 258, 460
Alw26I GTCTC 1 cut(s) 395
AlwNI CAGNNNCTG 1 cut(s) 164
AoxI GGCC 2 cut(s) 86, 481
ApoI RAATTY 2 cut(s) 300, 343
AspS9I GGNCC 1 cut(s) 349
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 1 cut(s) 349
BalI TGGCCA 1 cut(s) 88
BanII GRGCYC 2 cut(s) 258, 460
Bbv12I GWGCWC 2 cut(s) 258, 460
BccI CCATC 1 cut(s) 377
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 1 cut(s) 38
BglII AGATCT 1 cut(s) 518
BisI GCNGC 1 cut(s) 388
BlsI GCNGC 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 349
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 280
BmuI ACTGGG 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 3 cut(s) 359, 419, 512
BsaI GGTCTC 1 cut(s) 395
Bse1I ACTGG 2 cut(s) 89, 238
Bse3DI GCAATG 1 cut(s) 322
BseGI GGATG 1 cut(s) 27
BseMI GCAATG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 156, 322, 459
BseNI ACTGG 2 cut(s) 89, 238
BseRI GAGGAG 2 cut(s) 23, 296
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 88, 483
BsiHKAI GWGCWC 2 cut(s) 258, 460
BsmAI GTCTC 1 cut(s) 395
BsnI GGCC 2 cut(s) 88, 483
Bso31I GGTCTC 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 258, 460
Bsp143I GATC 1 cut(s) 518
BspACI CCGC 1 cut(s) 387
BspANI GGCC 2 cut(s) 88, 483
BspCNI CTCAG 3 cut(s) 157, 321, 460
BspFNI CGCG 1 cut(s) 504
BspTNI GGTCTC 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 322
BsrI ACTGG 2 cut(s) 89, 238
BssMI GATC 1 cut(s) 518
Bst4CI ACNGT 2 cut(s) 19, 271
Bst6I CTCTTC 1 cut(s) 528
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 3 cut(s) 165, 308, 468
BstF5I GGATG 1 cut(s) 27
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 1 cut(s) 521
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 1 cut(s) 518
BstMWI GCNNNNNNNGC 2 cut(s) 107, 170
BstUI CGCG 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 2 cut(s) 88, 483
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 502
CaiI CAGNNNCTG 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 349
CviAII CATG 1 cut(s) 9
CviJI RGCY 9 cut(s) 62, 88, 110, 122, 164, 256, 266, 458, 483
CviKI_1 RGCY 9 cut(s) 62, 88, 110, 122, 164, 256, 266, 458, 483
DdeI CTNAG 3 cut(s) 165, 308, 468
DpnI GATC 1 cut(s) 520
DpnII GATC 1 cut(s) 518
EaeI YGGCCR 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Ecl136II GAGCTC 2 cut(s) 256, 458
Eco24I GRGCYC 2 cut(s) 258, 460
Eco31I GGTCTC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 349
Eco53kI GAGCTC 2 cut(s) 256, 458
Eco57I CTGAAG 2 cut(s) 66, 260
EcoICRI GAGCTC 2 cut(s) 256, 458
EcoRI GAATTC 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 258, 460
FaeI CATG 1 cut(s) 12
FaiI YATR 5 cut(s) 10, 191, 364, 419, 441
FatI CATG 1 cut(s) 8
Fnu4HI GCNGC 1 cut(s) 388
FokI GGATG 1 cut(s) 14
FriOI GRGCYC 2 cut(s) 258, 460
Fsp4HI GCNGC 1 cut(s) 388
FspBI CTAG 1 cut(s) 38
GluI GCNGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 2 cut(s) 88, 483
Hin1II CATG 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 5 cut(s) 5, 41, 407, 436, 449
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 2 cut(s) 199, 322
Hpy188I TCNGA 4 cut(s) 46, 79, 311, 469
Hpy188III TCNNGA 3 cut(s) 212, 398, 453
Hpy8I GTNNAC 2 cut(s) 199, 322
HpyAV CCTTC 2 cut(s) 41, 227
HpyCH4III ACNGT 2 cut(s) 19, 271
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 4 cut(s) 101, 152, 293, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 170
HpyF3I CTNAG 3 cut(s) 165, 308, 468
HpySE526I ACGT 1 cut(s) 55
Hsp92II CATG 1 cut(s) 12
Kzo9I GATC 1 cut(s) 518
LmnI GCTCC 2 cut(s) 455, 463
LpnPI CCDG 5 cut(s) 70, 219, 263, 438, 529
LweI GCATC 1 cut(s) 280
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 520
MboI GATC 1 cut(s) 518
MboII GAAGA 2 cut(s) 266, 515
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 2 cut(s) 258, 460
MlsI TGGCCA 1 cut(s) 88
MluCI AATT 3 cut(s) 300, 343, 375
MluNI TGGCCA 1 cut(s) 88
MlyI GAGTC 2 cut(s) 14, 445
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 6 cut(s) 41, 44, 73, 305, 317, 362
Mox20I TGGCCA 1 cut(s) 88
MscI TGGCCA 1 cut(s) 88
Msp20I TGGCCA 1 cut(s) 88
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 2 cut(s) 107, 170
NdeII GATC 1 cut(s) 518
NlaIII CATG 1 cut(s) 12
PfeI GAWTC 3 cut(s) 41, 407, 449
PkrI GCNGC 1 cut(s) 389
PleI GAGTC 2 cut(s) 13, 444
PpsI GAGTC 2 cut(s) 13, 444
Psp124BI GAGCTC 2 cut(s) 258, 460
PspPI GGNCC 1 cut(s) 349
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 1 cut(s) 518
SacI GAGCTC 2 cut(s) 258, 460
SatI GCNGC 1 cut(s) 388
Sau3AI GATC 1 cut(s) 518
Sau96I GGNCC 1 cut(s) 349
SchI GAGTC 2 cut(s) 14, 445
SduI GDGCHC 2 cut(s) 258, 460
SfaNI GCATC 1 cut(s) 280
SinI GGWCC 1 cut(s) 349
SmlI CTYRAG 3 cut(s) 338, 398, 527
SmoI CTYRAG 3 cut(s) 338, 398, 527
Sse9I AATT 3 cut(s) 300, 343, 375
SsiI CCGC 1 cut(s) 387
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 2 cut(s) 258, 460
TaaI ACNGT 2 cut(s) 19, 271
TaiI ACGT 1 cut(s) 58
TaqII GACCGA 1 cut(s) 419
TasI AATT 3 cut(s) 300, 343, 375
TauI GCSGC 1 cut(s) 390
TfiI GAWTC 3 cut(s) 41, 407, 449
TspDTI ATGAA 2 cut(s) 261, 387
TspGWI ACGGA 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 2 cut(s) 300, 343
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.