MD10G1266600.v1.1

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008972
Physical Location & Seq
Forward (+)
35972783 .. 35974012
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1266600.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTCAGTGCTGAAATCATTTGGTGCAGTGTATCGTATAATGATTCAAGAGTCCTGCCCTTCGATAAAGAACATCCTCCTCCTCGATTCTGAAGGGAAACGTGTGGCTGTCAAGTATTATTCAGAGGAGGACTGGCCAACGAATACTGCCAAAGAATCTTTTGAGAAAGCTGTATTTACTAAAACTCAAAAGACAAATGCGCGGACAGAAGCTGAGATAGCAATGTTTGAGGGCACCATTGTAGTTTACAAGTTTGTTCAGGACCTTCACTTTTTTGTTACTGGTGGTGAAAATGAAAATGAGCTCATTTTAGCCACAGTTCTGCAGGGATTTTTTGATGCGGTTGGCATTCTCCTGAGAGGCAATGTGGACAAGAAGGAGGCACTTGAAAATTTGGATCTCATTTTACTATGCCTTGATGAAATTGTTGACGGGGGTAAGGTTGCAAGTCACAGTATTGATGCTGGAGCTCCGTTGTCCGAGCAGACAATAAGTCAAGCATTGGCAACTGCCCGCGAACATCTAGCGAGACAACTCCTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

182

Amino Acids

20.04

Weight (kDa)

5.04

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 22 - 152 4.7e-20 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccII CGCG 2 cut(s) 202, 516
AciI CCGC 3 cut(s) 202, 341, 514
AclWI GGATC 1 cut(s) 405
AcoI YGGCCR 1 cut(s) 134
AcsI RAATTY 1 cut(s) 391
AcuI CTGAAG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 2 cut(s) 47, 389
AhdI GACNNNNNGTC 1 cut(s) 492
AluBI AGCT 4 cut(s) 170, 212, 304, 470
AluI AGCT 4 cut(s) 170, 212, 304, 470
Alw21I GWGCWC 2 cut(s) 306, 472
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 1 cut(s) 405
AlwNI CAGNNNCTG 1 cut(s) 212
AoxI GGCC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 391
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 262
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 236
BalI TGGCCA 1 cut(s) 136
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 306, 472
Bbv12I GWGCWC 2 cut(s) 306, 472
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 1 cut(s) 323
Bme18I GGWCC 1 cut(s) 262
BmeRI GACNNNNNGTC 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 2 cut(s) 328, 451
BpmI CTGGAG 1 cut(s) 486
Bse1I ACTGG 2 cut(s) 137, 286
Bse3DI GCAATG 2 cut(s) 228, 370
BseGI GGATG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 228, 370
BseMII CTCAG 2 cut(s) 204, 347
BseNI ACTGG 2 cut(s) 137, 286
BseRI GAGGAG 3 cut(s) 68, 71, 140
BseSI GKGCMC 1 cut(s) 236
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 2 cut(s) 202, 516
BshFI GGCC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 2 cut(s) 306, 472
BsmAI GTCTC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 136
Bsp1286I GDGCHC 3 cut(s) 236, 306, 472
Bsp143I GATC 1 cut(s) 397
BspACI CCGC 3 cut(s) 202, 341, 514
BspANI GGCC 1 cut(s) 136
BspCNI CTCAG 2 cut(s) 205, 348
BspFNI CGCG 2 cut(s) 202, 516
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 2 cut(s) 228, 370
BsrI ACTGG 2 cut(s) 137, 286
BssMI GATC 1 cut(s) 397
Bst4CI ACNGT 2 cut(s) 319, 455
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 2 cut(s) 213, 356
BstF5I GGATG 1 cut(s) 72
BstFNI CGCG 2 cut(s) 202, 516
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 1 cut(s) 400
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 1 cut(s) 397
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 236
BstUI CGCG 2 cut(s) 202, 516
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 72
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 2 cut(s) 12, 33
Cac8I GCNNGC 1 cut(s) 514
CaiI CAGNNNCTG 1 cut(s) 212
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 262
CviJI RGCY 7 cut(s) 107, 136, 170, 212, 304, 314, 470
CviKI_1 RGCY 7 cut(s) 107, 136, 170, 212, 304, 314, 470
DdeI CTNAG 2 cut(s) 213, 356
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 134
Eam1105I GACNNNNNGTC 1 cut(s) 492
Ecl136II GAGCTC 2 cut(s) 304, 470
Eco24I GRGCYC 2 cut(s) 306, 472
Eco47I GGWCC 1 cut(s) 262
Eco53kI GAGCTC 2 cut(s) 304, 470
Eco57I CTGAAG 1 cut(s) 111
EcoICRI GAGCTC 2 cut(s) 304, 470
EcoO109I RGGNCCY 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 306, 472
FaiI YATR 2 cut(s) 38, 412
FauI CCCGC 1 cut(s) 521
FokI GGATG 1 cut(s) 59
FriOI GRGCYC 2 cut(s) 306, 472
FspBI CTAG 1 cut(s) 524
GlaI GCGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 1 cut(s) 136
HhaI GCGC 1 cut(s) 202
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 4 cut(s) 43, 50, 86, 155
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 247, 370, 430
Hpy188I TCNGA 3 cut(s) 91, 124, 481
Hpy188III TCNNGA 3 cut(s) 47, 260, 355
Hpy8I GTNNAC 3 cut(s) 247, 370, 430
HpyAV CCTTC 4 cut(s) 69, 86, 275, 370
HpyCH4III ACNGT 2 cut(s) 319, 455
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 3 cut(s) 26, 325, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 213, 356
HpySE526I ACGT 1 cut(s) 100
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 1 cut(s) 397
LmnI GCTCC 2 cut(s) 467, 475
LpnPI CCDG 7 cut(s) 67, 118, 245, 267, 311, 368, 450
LweI GCATC 2 cut(s) 328, 451
MaeI CTAG 1 cut(s) 524
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 2 cut(s) 277, 449
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MflI RGATCY 1 cut(s) 397
MhlI GDGCHC 3 cut(s) 236, 306, 472
MlsI TGGCCA 1 cut(s) 136
MluCI AATT 2 cut(s) 391, 423
MluNI TGGCCA 1 cut(s) 136
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 8 cut(s) 86, 89, 92, 118, 121, 223, 353, 373
Mox20I TGGCCA 1 cut(s) 136
MscI TGGCCA 1 cut(s) 136
MseI TTAA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 136
Mva1269I GAATGC 1 cut(s) 348
MvnI CGCG 2 cut(s) 202, 516
MwoI GCNNNNNNNGC 1 cut(s) 218
NdeII GATC 1 cut(s) 397
NlaIV GGNNCC 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 449
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 43, 86, 155
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PpuMI RGGWCCY 1 cut(s) 262
Psp124BI GAGCTC 2 cut(s) 306, 472
Psp5II RGGWCCY 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 262
PspPPI RGGWCCY 1 cut(s) 262
PstI CTGCAG 1 cut(s) 327
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 1 cut(s) 397
SacI GAGCTC 2 cut(s) 306, 472
SaqAI TTAA 1 cut(s) 540
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 262
SchI GAGTC 1 cut(s) 59
SduI GDGCHC 3 cut(s) 236, 306, 472
SetI ASST 7 cut(s) 103, 172, 214, 267, 306, 444, 472
SfaNI GCATC 2 cut(s) 328, 451
SfcI CTRYAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 262
Sse9I AATT 2 cut(s) 391, 423
SsiI CCGC 3 cut(s) 202, 341, 514
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 2 cut(s) 306, 472
TaaI ACNGT 2 cut(s) 319, 455
TaiI ACGT 1 cut(s) 103
TaqI TCGA 2 cut(s) 62, 84
TasI AATT 2 cut(s) 391, 423
TfiI GAWTC 3 cut(s) 43, 86, 155
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 2 cut(s) 12, 33
TseFI GTSAC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 2 cut(s) 309, 435
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 2 cut(s) 12, 33
VpaK11BI GGWCC 1 cut(s) 262
XapI RAATTY 1 cut(s) 391
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.