Rw5G008800

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008972
Physical Location & Seq
Reverse (-)
9499429 .. 9501897
2469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G008800.1

Sequence Viewer

Length: 555 bp
ATGATAAATTCACAAGCTTTAATTGAGTCATGCCCTACCATAAGGAACATCCTCCTCCTAGATTCTGAAGGAAAACGTATAGCTGTCAAGTATTATTCAGAGGACTGGCCAACGAATAGTGCAAAAGAAGCTTTTGAGAAAACTGTTTTCACTAAAACTCAAAAGACAAATGCGCGGACAGAAGCTGAGATAGCAATGTTTGAGAACAGCATAGTAGTTTACAAGTTTGTTCAAGACCTTCACTTTTTTGTTACCGGGGGTGATAATGAAAATGAACTCATTTTAGCCACAGTTCTTCAGGGATTTTTTGATGCAGTTGGAATTCTCCTCAGAGGCAATGTGGACAAAAAGGAGGCACTTGAGAATTTGGACCTCATTCTACTATGTCTTGATGAAATTGTTGATGGCGGCATTGTTCTTGAGACCGATTCAAATGTTATAGCAAGTAAGGTTGCGAGTCATAGTATTGATTCTGGAGCTCCGTTATCTGAGCAGACAATAGGCCAAGCACTTGCTACTGCACGTGAACATTTTGCAAGATCTCTTCTTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

184

Amino Acids

20.26

Weight (kDa)

4.75

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 13 - 153 7.5e-24 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 175
AciI CCGC 2 cut(s) 175, 408
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 3 cut(s) 7, 321, 364
AcuI CTGAAG 2 cut(s) 87, 281
AcvI CACGTG 1 cut(s) 524
AflII CTTAAG 1 cut(s) 548
AgsI TTSAA 2 cut(s) 233, 432
AluBI AGCT 5 cut(s) 17, 83, 131, 185, 479
AluI AGCT 5 cut(s) 17, 83, 131, 185, 479
Alw21I GWGCWC 1 cut(s) 481
Alw26I GTCTC 1 cut(s) 416
AlwNI CAGNNNCTG 1 cut(s) 185
AoxI GGCC 2 cut(s) 107, 502
ApoI RAATTY 3 cut(s) 7, 321, 364
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 370
BalI TGGCCA 1 cut(s) 109
BanII GRGCYC 1 cut(s) 481
BbrPI CACGTG 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 481
BccI CCATC 1 cut(s) 398
BcnI CCSGG 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 548
BglII AGATCT 1 cut(s) 539
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 370
BmrFI CCNGG 1 cut(s) 256
BmsI GCATC 1 cut(s) 301
BpmI CTGGAG 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 380, 440
BpuMI CCSGG 1 cut(s) 256
BsaAI YACGTR 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 2 cut(s) 201, 343
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 48
BseMI GCAATG 2 cut(s) 201, 343
BseMII CTCAG 3 cut(s) 177, 343, 480
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 2 cut(s) 44, 317
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 2 cut(s) 109, 504
BsiHKAI GWGCWC 1 cut(s) 481
BsiSI CCGG 1 cut(s) 255
BsmAI GTCTC 1 cut(s) 416
BsnI GGCC 2 cut(s) 109, 504
Bso31I GGTCTC 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 1 cut(s) 539
BspACI CCGC 2 cut(s) 175, 408
BspANI GGCC 2 cut(s) 109, 504
BspCNI CTCAG 3 cut(s) 178, 342, 481
BspFNI CGCG 1 cut(s) 175
BspTI CTTAAG 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 201, 343
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 1 cut(s) 539
Bst4CI ACNGT 2 cut(s) 145, 292
Bst6I CTCTTC 1 cut(s) 549
BstAFI CTTAAG 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 524
BstDEI CTNAG 3 cut(s) 186, 329, 489
BstF5I GGATG 1 cut(s) 48
BstFNI CGCG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 1 cut(s) 542
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 1 cut(s) 539
BstMWI GCNNNNNNNGC 2 cut(s) 128, 191
BstSCI CCNGG 1 cut(s) 254
BstUI CGCG 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 539
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 2 cut(s) 109, 504
BtsCI GGATG 1 cut(s) 48
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 370
CviAII CATG 1 cut(s) 30
CviJI RGCY 8 cut(s) 17, 83, 109, 131, 185, 287, 479, 504
CviKI_1 RGCY 8 cut(s) 17, 83, 109, 131, 185, 287, 479, 504
DdeI CTNAG 3 cut(s) 186, 329, 489
DpnI GATC 1 cut(s) 541
DpnII GATC 1 cut(s) 539
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
Ecl136II GAGCTC 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 479
Eco57I CTGAAG 2 cut(s) 87, 281
Eco72I CACGTG 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 481
FaeI CATG 1 cut(s) 33
FaiI YATR 7 cut(s) 31, 41, 80, 212, 385, 440, 462
FatI CATG 1 cut(s) 29
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 1 cut(s) 35
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 2 cut(s) 109, 504
HapII CCGG 1 cut(s) 255
HhaI GCGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 33
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 2 cut(s) 15, 129
HinfI GANTC 5 cut(s) 26, 62, 428, 457, 470
HpaII CCGG 1 cut(s) 255
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 3 cut(s) 220, 343, 527
Hpy188I TCNGA 4 cut(s) 67, 100, 332, 490
Hpy188III TCNNGA 4 cut(s) 233, 389, 419, 474
Hpy8I GTNNAC 3 cut(s) 220, 343, 527
HpyAV CCTTC 2 cut(s) 62, 248
HpyCH4III ACNGT 2 cut(s) 145, 292
HpyCH4IV ACGT 2 cut(s) 76, 523
HpyCH4V TGCA 4 cut(s) 122, 314, 521, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 191
HpyF3I CTNAG 3 cut(s) 186, 329, 489
HpySE526I ACGT 2 cut(s) 76, 523
Hsp92II CATG 1 cut(s) 33
HspAI GCGC 1 cut(s) 173
Kzo9I GATC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 476, 484
LpnPI CCDG 4 cut(s) 91, 268, 284, 459
LweI GCATC 1 cut(s) 301
MaeI CTAG 1 cut(s) 59
MaeII ACGT 2 cut(s) 76, 523
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 541
MboI GATC 1 cut(s) 539
MboII GAAGA 2 cut(s) 287, 536
MflI RGATCY 1 cut(s) 539
MhlI GDGCHC 1 cut(s) 481
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 5 cut(s) 7, 21, 321, 364, 396
MluNI TGGCCA 1 cut(s) 109
MlyI GAGTC 2 cut(s) 35, 466
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 7 cut(s) 62, 65, 94, 326, 338, 346, 383
Mox20I TGGCCA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 109
MseI TTAA 2 cut(s) 20, 549
Msp20I TGGCCA 1 cut(s) 109
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 1 cut(s) 255
MspR9I CCNGG 1 cut(s) 256
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 2 cut(s) 128, 191
NciI CCSGG 1 cut(s) 256
NdeII GATC 1 cut(s) 539
NlaIII CATG 1 cut(s) 33
PfeI GAWTC 3 cut(s) 62, 428, 470
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 2 cut(s) 34, 465
PmaCI CACGTG 1 cut(s) 524
PmlI CACGTG 1 cut(s) 524
PpsI GAGTC 2 cut(s) 34, 465
Ppu21I YACGTR 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 481
PspCI CACGTG 1 cut(s) 524
PspPI GGNCC 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 539
SacI GAGCTC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 20, 549
SatI GCNGC 1 cut(s) 409
Sau3AI GATC 1 cut(s) 539
Sau96I GGNCC 1 cut(s) 370
SchI GAGTC 2 cut(s) 35, 466
ScrFI CCNGG 1 cut(s) 256
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 1 cut(s) 301
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 3 cut(s) 359, 419, 548
SmoI CTYRAG 3 cut(s) 359, 419, 548
Sse9I AATT 5 cut(s) 7, 21, 321, 364, 396
SsiI CCGC 2 cut(s) 175, 408
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 481
StyD4I CCNGG 1 cut(s) 254
TaaI ACNGT 2 cut(s) 145, 292
TaiI ACGT 2 cut(s) 79, 526
TaqII GACCGA 1 cut(s) 440
TasI AATT 5 cut(s) 7, 21, 321, 364, 396
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 3 cut(s) 62, 428, 470
Tru1I TTAA 2 cut(s) 20, 549
Tru9I TTAA 2 cut(s) 20, 549
TspDTI ATGAA 3 cut(s) 282, 288, 408
TspGWI ACGGA 1 cut(s) 471
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 3 cut(s) 7, 321, 364
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.