MD05G1290800.v1.1

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008972
Physical Location & Seq
Forward (+)
42243255 .. 42245471
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290800.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGAGTCATGCCCTTCGATAAAGAACATCCTCCTCCTTGATTCTGAAGGGAAACGTGTGGCTGTCAAGTATTATTCAGAGGAGGACTGGCCAACGGATGCTGCCAAAGAATCTTTTGAGAAAGCTGTGTTTACTAAAACTCAAAAGACAAATGCACGGACAGAAGCTGAGATAGCAATGTTTGAGGGCACCATTGTAGTTTACAAGTTTGTTCAAGACCTTCACTTTTTTGTTACTGGGGGTGAAAATGAAAATGAGCTCATTTTAGCCACAGTTCTCCAGGGTTTTTTTGATGCAGTCGGCATTCTCCTGAGAGGCAATGTGGACAAGAAGGAAGCTCTTGAGAATTTGGATCTCATTTTACTATGCCTTGATGAAATTGTTGATGGCGGTATTGTTCTTGAGACTGATTCAAATGTTATAGCAAGTAAGGTTGCAAGTCATAGCATTGACGCTGGAGCTCCGTTGTCTGAGCAGACAATAAGTCAAGCATTGGCGACTGCCCGCGAACATCTAGCAAGATCACTTCTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

179

Amino Acids

19.52

Weight (kDa)

4.66

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 7 - 147 1.2e-22 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccII CGCG 1 cut(s) 507
AciI CCGC 2 cut(s) 390, 505
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 66
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 2 cut(s) 215, 414
AhdI GACNNNNNGTC 1 cut(s) 483
AjnI CCWGG 1 cut(s) 279
AluBI AGCT 5 cut(s) 125, 167, 259, 338, 461
AluI AGCT 5 cut(s) 125, 167, 259, 338, 461
Alw21I GWGCWC 2 cut(s) 261, 463
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 167
AoxI GGCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 101
ApoI RAATTY 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 254
BaeGI GKGCMC 1 cut(s) 191
BalI TGGCCA 1 cut(s) 91
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 2 cut(s) 261, 463
Bbv12I GWGCWC 2 cut(s) 261, 463
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 515
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
Bme1390I CCNGG 1 cut(s) 281
BmeRI GACNNNNNGTC 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 281
BmrI ACTGGG 1 cut(s) 246
BmsI GCATC 2 cut(s) 88, 283
BmuI ACTGGG 1 cut(s) 246
BpmI CTGGAG 2 cut(s) 263, 477
BpuEI CTTGAG 2 cut(s) 362, 422
BsaJI CCNNGG 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 92, 241
Bse3DI GCAATG 2 cut(s) 183, 325
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 27, 103
BseMI GCAATG 2 cut(s) 183, 325
BseMII CTCAG 3 cut(s) 159, 302, 462
BseNI ACTGG 2 cut(s) 92, 241
BseRI GAGGAG 2 cut(s) 23, 95
BseSI GKGCMC 1 cut(s) 191
BseXI GCAGC 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 507
BshFI GGCC 1 cut(s) 91
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 261, 463
BsmAI GTCTC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 303
BsnI GGCC 1 cut(s) 91
Bsp1286I GDGCHC 3 cut(s) 191, 261, 463
Bsp143I GATC 2 cut(s) 352, 521
BspACI CCGC 2 cut(s) 390, 505
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 3 cut(s) 160, 303, 463
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 183, 325
BsrI ACTGG 2 cut(s) 92, 241
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 2 cut(s) 352, 521
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 505
BstDEI CTNAG 3 cut(s) 168, 311, 471
BstF5I GGATG 2 cut(s) 27, 103
BstFNI CGCG 1 cut(s) 507
BstKTI GATC 2 cut(s) 355, 524
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 2 cut(s) 352, 521
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 191
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 91
BtsCI GGATG 2 cut(s) 27, 103
Cac8I GCNNGC 1 cut(s) 505
CaiI CAGNNNCTG 1 cut(s) 167
CseI GACGC 1 cut(s) 461
CviAII CATG 1 cut(s) 9
CviJI RGCY 8 cut(s) 62, 91, 125, 167, 259, 269, 338, 461
CviKI_1 RGCY 8 cut(s) 62, 91, 125, 167, 259, 269, 338, 461
DdeI CTNAG 3 cut(s) 168, 311, 471
DpnI GATC 2 cut(s) 354, 523
DpnII GATC 2 cut(s) 352, 521
DriI GACNNNNNGTC 1 cut(s) 483
EaeI YGGCCR 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 483
Ecl136II GAGCTC 2 cut(s) 259, 461
Eco24I GRGCYC 2 cut(s) 261, 463
Eco53kI GAGCTC 2 cut(s) 259, 461
Eco57I CTGAAG 1 cut(s) 66
EcoICRI GAGCTC 2 cut(s) 259, 461
EcoRII CCWGG 1 cut(s) 279
EcoT38I GRGCYC 2 cut(s) 261, 463
FaeI CATG 1 cut(s) 12
FaiI YATR 4 cut(s) 10, 367, 422, 444
FatI CATG 1 cut(s) 8
FauI CCCGC 1 cut(s) 512
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 2 cut(s) 14, 110
FriOI GRGCYC 2 cut(s) 261, 463
Fsp4HI GCNGC 1 cut(s) 102
FspBI CTAG 1 cut(s) 515
GluI GCNGC 1 cut(s) 102
GsuI CTGGAG 2 cut(s) 263, 477
HaeIII GGCC 1 cut(s) 91
HgaI GACGC 1 cut(s) 461
Hin1II CATG 1 cut(s) 12
HinfI GANTC 4 cut(s) 5, 41, 110, 410
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 3 cut(s) 132, 202, 325
Hpy188I TCNGA 3 cut(s) 46, 79, 472
Hpy188III TCNNGA 4 cut(s) 215, 310, 341, 401
Hpy8I GTNNAC 3 cut(s) 132, 202, 325
HpyAV CCTTC 4 cut(s) 24, 41, 230, 325
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 55
HpyCH4V TGCA 3 cut(s) 155, 296, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 3 cut(s) 168, 311, 471
HpySE526I ACGT 1 cut(s) 55
Hsp92II CATG 1 cut(s) 12
Kzo9I GATC 2 cut(s) 352, 521
LmnI GCTCC 2 cut(s) 458, 466
LpnPI CCDG 6 cut(s) 73, 222, 266, 293, 323, 441
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 2 cut(s) 88, 283
MaeI CTAG 1 cut(s) 515
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 1 cut(s) 232
MalI GATC 2 cut(s) 354, 523
MboI GATC 2 cut(s) 352, 521
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 191, 261, 463
MlsI TGGCCA 1 cut(s) 91
MluCI AATT 2 cut(s) 346, 378
MluNI TGGCCA 1 cut(s) 91
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 6 cut(s) 41, 44, 73, 76, 178, 308
Mox20I TGGCCA 1 cut(s) 91
MscI TGGCCA 1 cut(s) 91
MseI TTAA 1 cut(s) 531
Msp20I TGGCCA 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 281
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 2 cut(s) 352, 521
NlaIII CATG 1 cut(s) 12
NlaIV GGNNCC 1 cut(s) 190
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 41, 110, 410
PkrI GCNGC 1 cut(s) 103
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp124BI GAGCTC 2 cut(s) 261, 463
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 1 cut(s) 190
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 352
SacI GAGCTC 2 cut(s) 261, 463
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 2 cut(s) 352, 521
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 3 cut(s) 191, 261, 463
SetI ASST 8 cut(s) 58, 127, 169, 222, 261, 340, 435, 463
SfaNI GCATC 2 cut(s) 88, 283
SmlI CTYRAG 2 cut(s) 341, 401
SmoI CTYRAG 2 cut(s) 341, 401
Sse9I AATT 2 cut(s) 346, 378
SsiI CCGC 2 cut(s) 390, 505
SspMI CTAG 1 cut(s) 515
SstI GAGCTC 2 cut(s) 261, 463
StyD4I CCNGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 58
TaqI TCGA 1 cut(s) 17
TasI AATT 2 cut(s) 346, 378
TfiI GAWTC 3 cut(s) 41, 110, 410
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 2 cut(s) 264, 390
TspGWI ACGGA 3 cut(s) 110, 172, 453
XapI RAATTY 1 cut(s) 346
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.