Rorug05G0006400

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008972
Physical Location & Seq
Forward (+)
391407 .. 391979
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0006400.1

Sequence Viewer

Length: 471 bp
ATGCGTATAATCAGTGGTCCAAAACATGGAGATGGAGATGGAGATGGATCAGATGAAAATGGGGATGGAGAAGGATCAGATGGAGATGGAGATGGAGATGGTGATGGATCAGATGGAAATGGAAATGGAGATGGATCAGATGGGGGTGACATGAATCGAGTTGGGAAAGCATGCTCAAAGGACGACATAACAATCTTCCAAGGGGAAACGGCTCCACTCCCAAGTGGAATTCCGGCTTACACAGTTCAGATCCTCAACACGTGCGTCTCAGGTTGCAGCATCTCTGACATTCACATCAACTGTGGATGGTTTAGCTCCGCCCGCCTTGTCAATCCTAGAGTCTTCAGGCGCATGGACTACAACGACTGCCTAGTCAATGATGGTCAGCCTCTTGGCCCTGGACAAACCCTCTCTTTCCAATATGCCAATAGCTTTCGTTACCCGCTTTCTGTTTCATCTGTAGCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

156

Amino Acids

16.16

Weight (kDa)

4.15

Isoelectric Point (pI)

26.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 59 - 156 6.5e-43 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 26
AciI CCGC 3 cut(s) 318, 322, 443
AclWI GGATC 5 cut(s) 55, 82, 115, 142, 244
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 328
AcvI CACGTG 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 258
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 3 cut(s) 315, 432, 464
AluI AGCT 3 cut(s) 315, 432, 464
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 5 cut(s) 55, 82, 115, 142, 244
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 1 cut(s) 276
ApoI RAATTY 1 cut(s) 228
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 17, 395
AsuHPI GGTGA 2 cut(s) 113, 158
AvaII GGWCC 1 cut(s) 17
BbrPI CACGTG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 288
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 2 cut(s) 336, 371
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 1 cut(s) 277
BlsI GCNGC 1 cut(s) 278
Bme1390I CCNGG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 17, 395
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 1 cut(s) 288
BpiI GAAGAC 1 cut(s) 334
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 199, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 26
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 199, 397
BseGI GGATG 2 cut(s) 70, 311
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 1 cut(s) 282
BseXI GCAGC 1 cut(s) 288
BshFI GGCC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 271
BsmBI CGTCTC 1 cut(s) 271
BsnI GGCC 1 cut(s) 396
Bsp143I GATC 5 cut(s) 47, 74, 107, 134, 249
BspACI CCGC 3 cut(s) 318, 322, 443
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 281
BspLI GGNNCC 1 cut(s) 213
BspPI GGATC 5 cut(s) 55, 82, 115, 142, 244
BssECI CCNNGG 2 cut(s) 199, 397
BssMI GATC 5 cut(s) 47, 74, 107, 134, 249
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 244, 302
BstBAI YACGTR 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 172, 322
BstDEI CTNAG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 70, 311
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 5 cut(s) 50, 77, 110, 137, 252
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 5 cut(s) 47, 74, 107, 134, 249
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 1 cut(s) 288
BstV2I GAAGAC 1 cut(s) 334
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BsuRI GGCC 1 cut(s) 396
BtsCI GGATG 2 cut(s) 70, 311
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 172, 322
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 17, 395
CseI GACGC 1 cut(s) 253
CviAII CATG 4 cut(s) 26, 151, 171, 352
CviJI RGCY 7 cut(s) 212, 236, 315, 388, 396, 432, 464
CviKI_1 RGCY 7 cut(s) 212, 236, 315, 388, 396, 432, 464
DdeI CTNAG 1 cut(s) 268
DpnI GATC 5 cut(s) 49, 76, 109, 136, 251
DpnII GATC 5 cut(s) 47, 74, 107, 134, 249
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EciI GGCGGA 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 17
Eco57I CTGAAG 1 cut(s) 328
Eco72I CACGTG 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 199
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 4 cut(s) 29, 154, 174, 355
FaiI YATR 7 cut(s) 8, 27, 152, 172, 188, 353, 423
FatI CATG 4 cut(s) 25, 150, 170, 351
FauI CCCGC 2 cut(s) 329, 450
Fnu4HI GCNGC 1 cut(s) 277
FokI GGATG 2 cut(s) 77, 318
Fsp4HI GCNGC 1 cut(s) 277
FspBI CTAG 2 cut(s) 336, 371
GlaI GCGC 1 cut(s) 350
GluI GCNGC 1 cut(s) 277
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 351
Hin1II CATG 4 cut(s) 29, 154, 174, 355
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 2 cut(s) 154, 339
HpaII CCGG 1 cut(s) 233
HphI GGTGA 2 cut(s) 113, 158
Hpy188I TCNGA 6 cut(s) 52, 79, 112, 139, 249, 286
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 2 cut(s) 244, 302
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 1 cut(s) 268
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 4 cut(s) 29, 154, 174, 355
HspAI GCGC 1 cut(s) 349
Kzo9I GATC 5 cut(s) 47, 74, 107, 134, 249
LmnI GCTCC 2 cut(s) 217, 320
LpnPI CCDG 5 cut(s) 246, 255, 331, 384, 411
Lsp1109I GCAGC 1 cut(s) 288
LweI GCATC 1 cut(s) 288
MaeI CTAG 2 cut(s) 336, 371
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 2 cut(s) 146, 437
MalI GATC 5 cut(s) 49, 76, 109, 136, 251
MboI GATC 5 cut(s) 47, 74, 107, 134, 249
MboII GAAGA 2 cut(s) 187, 334
MflI RGATCY 1 cut(s) 249
MluCI AATT 1 cut(s) 228
MlyI GAGTC 1 cut(s) 348
MnlI CCTC 3 cut(s) 263, 399, 419
MslI CAYNNNNRTG 1 cut(s) 30
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 321
NdeII GATC 5 cut(s) 47, 74, 107, 134, 249
NlaIII CATG 4 cut(s) 29, 154, 174, 355
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 1 cut(s) 174
PaeI GCATGC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 154
PflMI CCANNNNNTGG 1 cut(s) 26
PkrI GCNGC 1 cut(s) 278
PleI GAGTC 1 cut(s) 347
PmaCI CACGTG 1 cut(s) 261
PmlI CACGTG 1 cut(s) 261
PpsI GAGTC 1 cut(s) 347
Ppu21I YACGTR 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 397
PspCI CACGTG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 2 cut(s) 17, 395
PsuI RGATCY 1 cut(s) 249
RseI CAYNNNNRTG 1 cut(s) 30
SatI GCNGC 1 cut(s) 277
Sau3AI GATC 5 cut(s) 47, 74, 107, 134, 249
Sau96I GGNCC 2 cut(s) 17, 395
SchI GAGTC 1 cut(s) 348
ScrFI CCNGG 1 cut(s) 399
SetI ASST 5 cut(s) 263, 274, 317, 434, 466
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 30
SphI GCATGC 1 cut(s) 174
Sse9I AATT 1 cut(s) 228
SsiI CCGC 3 cut(s) 318, 322, 443
SspMI CTAG 2 cut(s) 336, 371
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 2 cut(s) 244, 302
TaiI ACGT 1 cut(s) 263
TaqI TCGA 1 cut(s) 157
TasI AATT 1 cut(s) 228
TfiI GAWTC 1 cut(s) 154
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 1 cut(s) 276
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 3 cut(s) 69, 167, 444
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 174
XspI CTAG 2 cut(s) 336, 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.