FvH4_3g08250

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008757
Physical Location & Seq
Reverse (-)
4835183 .. 4837425
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08250.t1

Sequence Viewer

Length: 729 bp
ATGCGAAGAGCCTCAAGGATCATTACGTCATCAGCTTCAAACCTCCGCAACTCTGCTTCACCTAGTTTTGCTCTGCCCAGAAACTCCTTCACTACTTTGATTCAAAACCCAGAAAGGCAATCCCTTTCCGGTGGTTTTCTGAAATGGGGTTCGCTTGGTTCGGTTAGAGCTTCCAGTTTTGCTTCTGGGTTCTCGCCCTTGAAGCCCAAGCCCTTGGATTCCATTATCGACATTGATAGAGTAAGGGATCGCTCACCTGAAGACATTGCTTCGGCTTGGGATGATTATCACTTGGGGAGAGGACATATTGGTGCATCAATGAAAGCGAGCCTGTATCACTTGTTGGAGCATAGAGCAGCAGATTGCCGGTATTTCGTCATTCCTTTGTGGAGAGGGAGTGGTTATGTCACAATGTTTGCTCAAGTGCAGATGCCACATATGATTTTCACTGGTCTTGAAGATTATAAGGCAAGAGGAACTCAAGCTACTCCCTACTTCACTATGACGTATTACAATGAATTTTCACAAAGCAAGGACATGGTGCTTATCCGAGGCGATGTTGTACTACCCAGCAAGCTCAGTGACTCAGAAGCAAAATGGCTTCTGGAGACCACCCAAACCTTCTATTTGAATGATGTCAGGTACAAGTTGGTTGAACGGTTCAACAAACAAACACATGATTTCGAATTCAAGGATGTCTTGCAAGCACTGGATATGCCGAATCTGTGA

Protein Analysis

243

Amino Acids

27.55

Weight (kDa)

9.12

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP11 PF06644 65 - 233 1.1e-37 ATP11 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
AciI CCGC 1 cut(s) 46
AclWI GGATC 2 cut(s) 26, 255
AcsI RAATTY 2 cut(s) 518, 686
AcuI CTGAAG 1 cut(s) 279
AfaI GTAC 2 cut(s) 564, 644
AgsI TTSAA 8 cut(s) 39, 104, 202, 458, 631, 656, 664, 691
AluBI AGCT 4 cut(s) 35, 170, 485, 577
AluI AGCT 4 cut(s) 35, 170, 485, 577
Alw26I GTCTC 1 cut(s) 602
AlwI GGATC 2 cut(s) 26, 255
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 518, 686
AsuHPI GGTGA 2 cut(s) 51, 246
AsuII TTCGAA 1 cut(s) 684
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 1 cut(s) 63
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmsI GCATC 2 cut(s) 323, 420
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 684
BpuEI CTTGAG 2 cut(s) 405, 465
BsaBI GATNNNNATC 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 2 cut(s) 213, 550
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse118I RCCGGY 1 cut(s) 366
Bse1I ACTGG 3 cut(s) 174, 454, 714
Bse3DI GCAATG 1 cut(s) 264
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 213, 550
BseGI GGATG 2 cut(s) 286, 700
BseJI GATNNNNATC 1 cut(s) 285
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 592, 600
BseNI ACTGG 3 cut(s) 174, 454, 714
BseXI GCAGC 1 cut(s) 368
BseYI CCCAGC 1 cut(s) 569
BsgI GTGCAG 1 cut(s) 446
BsiSI CCGG 2 cut(s) 129, 367
BsmAI GTCTC 1 cut(s) 602
Bso31I GGTCTC 1 cut(s) 602
Bsp119I TTCGAA 1 cut(s) 684
Bsp143I GATC 2 cut(s) 18, 247
BspACI CCGC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 591, 599
BspPI GGATC 2 cut(s) 26, 255
BspT104I TTCGAA 1 cut(s) 684
BspTNI GGTCTC 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 366
BsrI ACTGG 3 cut(s) 174, 454, 714
BssAI RCCGGY 1 cut(s) 366
BssECI CCNNGG 2 cut(s) 213, 550
BssMI GATC 2 cut(s) 18, 247
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 660
BstBI TTCGAA 1 cut(s) 684
BstC8I GCNNGC 3 cut(s) 328, 575, 705
BstDEI CTNAG 2 cut(s) 578, 586
BstF5I GGATG 2 cut(s) 286, 700
BstKTI GATC 2 cut(s) 21, 250
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 2 cut(s) 18, 247
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 368
BstV2I GAAGAC 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 214
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 2 cut(s) 286, 700
BtsIMutI CAGTG 3 cut(s) 447, 586, 707
Cac8I GCNNGC 3 cut(s) 328, 575, 705
Cfr10I RCCGGY 1 cut(s) 366
Csp6I GTAC 2 cut(s) 563, 643
CviAII CATG 2 cut(s) 538, 677
CviQI GTAC 2 cut(s) 563, 643
DdeI CTNAG 2 cut(s) 578, 586
DpnI GATC 2 cut(s) 20, 249
DpnII GATC 2 cut(s) 18, 247
Eco130I CCWWGG 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 279
EcoRI GAATTC 1 cut(s) 686
EcoT14I CCWWGG 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 541, 680
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FatI CATG 2 cut(s) 537, 676
FauNDI CATATG 1 cut(s) 438
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 293, 707
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 1 cut(s) 63
GluI GCNGC 1 cut(s) 357
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 1 cut(s) 626
HapII CCGG 2 cut(s) 129, 367
Hin1II CATG 2 cut(s) 541, 680
HinfI GANTC 4 cut(s) 100, 218, 584, 721
HpaII CCGG 2 cut(s) 129, 367
HphI GGTGA 2 cut(s) 51, 246
Hpy188I TCNGA 3 cut(s) 141, 551, 589
Hpy188III TCNNGA 2 cut(s) 455, 605
HpyAV CCTTC 2 cut(s) 97, 631
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4IV ACGT 2 cut(s) 26, 506
HpyCH4V TGCA 3 cut(s) 314, 427, 703
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 2 cut(s) 578, 586
HpySE526I ACGT 2 cut(s) 26, 506
Hsp92II CATG 2 cut(s) 541, 680
Kzo9I GATC 2 cut(s) 18, 247
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 2 cut(s) 323, 420
MaeI CTAG 1 cut(s) 63
MaeII ACGT 2 cut(s) 26, 506
MaeIII GTNAC 2 cut(s) 406, 581
MalI GATC 2 cut(s) 20, 249
MboI GATC 2 cut(s) 18, 247
MboII GAAGA 3 cut(s) 18, 272, 470
MluCI AATT 2 cut(s) 518, 686
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 6 cut(s) 22, 53, 293, 386, 467, 545
MslI CAYNNNNRTG 1 cut(s) 309
MspI CCGG 2 cut(s) 129, 367
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeI CATATG 1 cut(s) 438
NdeII GATC 2 cut(s) 18, 247
NlaIII CATG 2 cut(s) 541, 680
NmuCI GTSAC 2 cut(s) 406, 581
NspV TTCGAA 1 cut(s) 684
PfeI GAWTC 3 cut(s) 100, 218, 721
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PsiI TTATAA 1 cut(s) 465
PspFI CCCAGC 1 cut(s) 569
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
RsaI GTAC 2 cut(s) 564, 644
RsaNI GTAC 2 cut(s) 563, 643
RseI CAYNNNNRTG 1 cut(s) 309
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 2 cut(s) 18, 247
SchI GAGTC 1 cut(s) 578
SfaNI GCATC 2 cut(s) 323, 420
SfuI TTCGAA 1 cut(s) 684
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 3 cut(s) 13, 420, 480
SmoI CTYRAG 3 cut(s) 13, 420, 480
Sse9I AATT 2 cut(s) 518, 686
SsiI CCGC 1 cut(s) 46
SspMI CTAG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 660
TaiI ACGT 2 cut(s) 29, 509
TaqI TCGA 2 cut(s) 228, 684
TasI AATT 2 cut(s) 518, 686
TatI WGTACW 1 cut(s) 562
TfiI GAWTC 3 cut(s) 100, 218, 721
TscAI CASTG 3 cut(s) 454, 586, 714
TseFI GTSAC 2 cut(s) 406, 581
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 406, 581
TspDTI ATGAA 2 cut(s) 335, 531
TspRI CASTG 3 cut(s) 454, 586, 714
XapI RAATTY 2 cut(s) 518, 686
XspI CTAG 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.