MD10G1285300.v1.1

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008757
Physical Location & Seq
Reverse (-)
37533846 .. 37536502
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1285300.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCGAAGAGCTTCCAGGATCTTTACGCTTCCAGCTTCAAACCTCATCGGCCATTCTTCTGCTTCTACTGGCGCTTTGCACAAACACTCCTTCACAACTCTGACCCTGCAACAAAATCGCCAAAACCCACAACAGCAATCGTTTCCGGGAGGATTTCTGAAATGGGGTTCGCTGGGTTTCTTGAGGGCTTTGAGTTTTGCTGCTGGGTTCAATCCCATGAAGGCTAAGCCGTTGGATTCCATTATTGATGTCGAGAGGGTGAAAGATCGCTCGGCCGAGGACATCGCTTCGGCTTGGGACGATTTTCACCTGGGAAGAGGTCATATCGGTGCATCCATGAATGCAAAACTATATCACCTGTTGGAGCATAGAGCGGCAGATTGCCGGTATTTTGTCATTCCCTTGTGGAGAGGAAATGGTTATACCACAATGTTTGCTCAAGTACAGATGCCACACATGGTTTTCACTGGTCTTGAAGATTACAAGGCGAGGGGAACTCAAGCTTCTCCCTACTTCGCTGTGACTTACTACAACGAATTTGCAGAAAGCAAGGACATGGTGCTTATTCGAGGGGATGTCGTACTCCCCAGCAAACTCACTGACTCGGAGGCAAAATGGCTTGTGGAGACCACCCAATCATTTTATTTGAACGACATGAGGTATAAGCTGGTCGAACGGTTCAACAAACAAACACACGATTTTGAGTTCAAGGATGTCTTGCAAGCATTAGAAATGCCGATTCTGTGA

Protein Analysis

249

Amino Acids

28.27

Weight (kDa)

8.89

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP11 PF06644 69 - 239 1.5e-38 ATP11 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 374
AciI CCGC 1 cut(s) 374
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 2 cut(s) 49, 273
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 2 cut(s) 444, 582
AgsI TTSAA 6 cut(s) 39, 211, 476, 649, 682, 709
AjnI CCWGG 2 cut(s) 14, 309
AluBI AGCT 4 cut(s) 11, 35, 503, 667
AluI AGCT 4 cut(s) 11, 35, 503, 667
Alw26I GTCTC 1 cut(s) 620
AlwI GGATC 1 cut(s) 26
AoxI GGCC 2 cut(s) 49, 273
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 3 cut(s) 271, 299, 347
BbvI GCAGC 1 cut(s) 187
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 2 cut(s) 16, 311
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 620
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 2 cut(s) 201, 375
BlpI GCTNAGC 1 cut(s) 225
BlsI GCNGC 2 cut(s) 202, 376
Bme1390I CCNGG 3 cut(s) 16, 147, 311
BmrFI CCNGG 3 cut(s) 16, 147, 311
BmsI GCATC 2 cut(s) 341, 438
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu1102I GCTNAGC 1 cut(s) 225
BpuEI CTTGAG 3 cut(s) 202, 423, 483
BpuMI CCSGG 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 2 cut(s) 276, 310
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse118I RCCGGY 1 cut(s) 384
Bse1I ACTGG 2 cut(s) 73, 472
BseBI CCWGG 2 cut(s) 16, 311
BseDI CCNNGG 2 cut(s) 276, 310
BseGI GGATG 3 cut(s) 332, 580, 718
BseNI ACTGG 2 cut(s) 73, 472
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 3 cut(s) 172, 203, 587
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 2 cut(s) 51, 275
BsiEI CGRYCG 1 cut(s) 276
BsiSI CCGG 2 cut(s) 146, 385
BslFI GGGAC 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 51, 275
Bso31I GGTCTC 1 cut(s) 620
Bsp143I GATC 2 cut(s) 18, 265
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 1 cut(s) 374
BspANI GGCC 2 cut(s) 51, 275
BspPI GGATC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 620
BsrBI CCGCTC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 384
BsrI ACTGG 2 cut(s) 73, 472
BssAI RCCGGY 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 276, 310
BssMI GATC 2 cut(s) 18, 265
Bst2UI CCWGG 2 cut(s) 16, 311
Bst4CI ACNGT 1 cut(s) 678
Bst6I CTCTTC 1 cut(s) 310
BstC8I GCNNGC 1 cut(s) 723
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 3 cut(s) 332, 580, 718
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 2 cut(s) 21, 268
BstMAI GTCTC 1 cut(s) 620
BstMBI GATC 2 cut(s) 18, 265
BstMCI CGRYCG 1 cut(s) 276
BstNI CCWGG 2 cut(s) 16, 311
BstSCI CCNGG 3 cut(s) 14, 145, 309
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 2 cut(s) 51, 275
BtgZI GCGATG 1 cut(s) 268
BtsCI GGATG 3 cut(s) 332, 580, 718
BtsIMutI CAGTG 2 cut(s) 465, 597
Cac8I GCNNGC 1 cut(s) 723
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 384
Csp6I GTAC 2 cut(s) 443, 581
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 5 cut(s) 217, 337, 457, 556, 655
CviQI GTAC 2 cut(s) 443, 581
DdeI CTNAG 1 cut(s) 225
DpnI GATC 2 cut(s) 20, 267
DpnII GATC 2 cut(s) 18, 265
EaeI YGGCCR 2 cut(s) 49, 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
EclXI CGGCCG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 273
EcoRII CCWGG 2 cut(s) 14, 309
FaeI CATG 5 cut(s) 220, 340, 460, 559, 658
FalI AAGNNNNNCTT 2 cut(s) 701, 733
FaqI GGGAC 1 cut(s) 311
FatI CATG 5 cut(s) 216, 336, 456, 555, 654
Fnu4HI GCNGC 2 cut(s) 201, 375
FokI GGATG 3 cut(s) 319, 587, 725
Fsp4HI GCNGC 2 cut(s) 201, 375
GlaI GCGC 1 cut(s) 73
GluI GCNGC 2 cut(s) 201, 375
GsaI CCCAGC 3 cut(s) 176, 207, 591
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 51, 275
HapII CCGG 2 cut(s) 146, 385
HhaI GCGC 1 cut(s) 74
Hin1II CATG 5 cut(s) 220, 340, 460, 559, 658
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 3 cut(s) 236, 602, 739
HpaII CCGG 2 cut(s) 146, 385
HphI GGTGA 3 cut(s) 271, 299, 347
Hpy188I TCNGA 3 cut(s) 102, 159, 607
Hpy188III TCNNGA 3 cut(s) 181, 253, 473
HpyAV CCTTC 2 cut(s) 100, 214
HpyCH4III ACNGT 1 cut(s) 678
HpyCH4V TGCA 6 cut(s) 79, 109, 332, 344, 542, 721
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 5 cut(s) 220, 340, 460, 559, 658
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 2 cut(s) 18, 265
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 2 cut(s) 341, 438
MaeIII GTNAC 1 cut(s) 520
MalI GATC 2 cut(s) 20, 267
MbiI CCGCTC 1 cut(s) 374
MboI GATC 2 cut(s) 18, 265
MboII GAAGA 4 cut(s) 18, 48, 327, 488
MflI RGATCY 1 cut(s) 18
MluCI AATT 1 cut(s) 536
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 2 cut(s) 213, 342
MroXI GAANNNNTTC 1 cut(s) 10
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 2 cut(s) 146, 385
MspR9I CCNGG 3 cut(s) 16, 147, 311
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 2 cut(s) 16, 311
NciI CCSGG 1 cut(s) 147
NdeII GATC 2 cut(s) 18, 265
NlaIII CATG 5 cut(s) 220, 340, 460, 559, 658
NmeAIII GCCGAG 2 cut(s) 251, 301
NmuCI GTSAC 1 cut(s) 520
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 236, 739
PfoI TCCNGGA 2 cut(s) 14, 145
PkrI GCNGC 2 cut(s) 202, 376
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
Psp6I CCWGG 2 cut(s) 14, 309
PspFI CCCAGC 3 cut(s) 172, 203, 587
PspGI CCWGG 2 cut(s) 14, 309
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 2 cut(s) 444, 582
RsaNI GTAC 2 cut(s) 443, 581
RseI CAYNNNNRTG 1 cut(s) 327
SatI GCNGC 2 cut(s) 201, 375
Sau3AI GATC 2 cut(s) 18, 265
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 3 cut(s) 16, 147, 311
SetI ASST 9 cut(s) 13, 37, 45, 312, 322, 360, 505, 662, 669
SfaNI GCATC 2 cut(s) 341, 438
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 3 cut(s) 181, 438, 498
SmoI CTYRAG 3 cut(s) 181, 438, 498
Sse9I AATT 1 cut(s) 536
SsiI CCGC 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 14, 145, 309
TaaI ACNGT 1 cut(s) 678
TaqI TCGA 3 cut(s) 252, 568, 672
TasI AATT 1 cut(s) 536
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 236, 739
TscAI CASTG 2 cut(s) 472, 604
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 2 cut(s) 233, 353
TspRI CASTG 2 cut(s) 472, 604
XapI RAATTY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.