MD05G1306300.v1.1

ATP synthase mitochondrial F1 complex assembly factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008757
Physical Location & Seq
Reverse (-)
43841741 .. 43844369
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1306300.v1.1.491

Sequence Viewer

Length: 744 bp
ATGCGAAGAGCCTCCAGAATCATTACGCTTCCAGCTTCGATCCTCATCGGCCATTCTTCTTCTGCTGGTGCTGTGCACAACCACTCCTTCACATCTCTGACCCTGCTACAAAATCGCCGCAACTCGCAACAACAATCGTTCCCGGGAGATTTTCTGAAATGGGGTTCGCTAGGTTTCTTCAGGGCTTCGAGCTTCGCTACTGGATTCAATCCCTTGAAGGCTAAGCCGTTGGATTCAATCATTGATGTTGAGAGGGTGAAAGATCGCTCGGCCGAGGACATCGCTTCCGCTTGGGACGATTTTCACCTGGGAAGAGGTCATATTGGTGCATCCATGAAAGCAAAACTATATAACCTGTTGGAGCATAGAGCGGTAGATTGCCGGTATTTTGTCATTCCCTTGTGGAGAGGAAATGGTTATACCACAATGTTTGCTCAAGTACAGATGCCACACATGATTTTCACCGGTCTTGAAGATTACAAGGCGAGGGGAACTCAAGCTTCTCCCTACTTCGCTGTAACTTACTACAACGAATTTGCAGAAAGCAAGGACATGGTGCTTATCCGAGGGGATGTCGTACTCCCAAGCAAACTCACTGACTCGGAGGCAAAATGGCTCGTGGAGACCACCCAATCATTTTATTTGAACGACATGAGGTATAAGCTGGTCGAACGGTTCAACAAACAAACACACGATTTTGAGTTCAAGGATGTCCTGCAAGCTTTAGAAATGCCGAATCTGTGA

Protein Analysis

248

Amino Acids

28.2

Weight (kDa)

8.89

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP11 PF06644 70 - 238 1.7e-38 ATP11 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 371
AciI CCGC 3 cut(s) 118, 288, 371
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 2 cut(s) 49, 270
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 163
AfaI GTAC 2 cut(s) 441, 579
AgeI ACCGGT 1 cut(s) 464
AgsI TTSAA 7 cut(s) 208, 217, 237, 473, 646, 679, 706
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 5 cut(s) 35, 192, 500, 664, 722
AluI AGCT 5 cut(s) 35, 192, 500, 664, 722
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 617
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 142
AoxI GGCC 2 cut(s) 49, 270
ApaLI GTGCAC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 533
AsiGI ACCGGT 1 cut(s) 464
AsuC2I CCSGG 2 cut(s) 143, 144
AsuHPI GGTGA 3 cut(s) 268, 296, 454
AvaI CYCGRG 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 78
BauI CACGAG 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 308
BcnI CCSGG 2 cut(s) 143, 144
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 1 cut(s) 170
BisI GCNGC 1 cut(s) 118
BlpI GCTNAGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 3 cut(s) 143, 144, 308
BmeT110I CYCGRG 1 cut(s) 142
BmrFI CCNGG 3 cut(s) 143, 144, 308
BmsI GCATC 2 cut(s) 338, 435
BplI GAGNNNNNCTC 2 cut(s) 478, 510
Bpu1102I GCTNAGC 1 cut(s) 222
BpuEI CTTGAG 2 cut(s) 420, 480
BpuMI CCSGG 2 cut(s) 143, 144
BsaBI GATNNNNATC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 4 cut(s) 142, 273, 307, 565
BsaWI WCCGGW 1 cut(s) 464
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse118I RCCGGY 2 cut(s) 381, 464
Bse1I ACTGG 1 cut(s) 205
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 4 cut(s) 142, 273, 307, 565
BseGI GGATG 3 cut(s) 329, 577, 715
BseJI GATNNNNATC 1 cut(s) 44
BseNI ACTGG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 78
BseX3I CGGCCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 2 cut(s) 51, 272
BshTI ACCGGT 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 3 cut(s) 143, 382, 465
BslFI GGGAC 1 cut(s) 308
BsmAI GTCTC 1 cut(s) 617
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 2 cut(s) 51, 272
Bso31I GGTCTC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 39, 262
Bsp1720I GCTNAGC 1 cut(s) 222
BspACI CCGC 3 cut(s) 118, 288, 371
BspANI GGCC 2 cut(s) 51, 272
BspPI GGATC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 617
BsrBI CCGCTC 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 381, 464
BsrI ACTGG 1 cut(s) 205
BssAI RCCGGY 2 cut(s) 381, 464
BssECI CCNNGG 4 cut(s) 142, 273, 307, 565
BssMI GATC 2 cut(s) 39, 262
BssSI CACGAG 1 cut(s) 617
Bst2BI CACGAG 1 cut(s) 617
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 675
Bst6I CTCTTC 1 cut(s) 307
BstC8I GCNNGC 1 cut(s) 720
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 329, 577, 715
BstKTI GATC 2 cut(s) 42, 265
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 2 cut(s) 39, 262
BstMCI CGRYCG 1 cut(s) 273
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 3 cut(s) 141, 142, 306
BstSLI GKGCMC 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 51, 272
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 3 cut(s) 329, 577, 715
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 720
Cfr10I RCCGGY 2 cut(s) 381, 464
Cfr9I CCCGGG 1 cut(s) 142
Csp6I GTAC 2 cut(s) 440, 578
CspAI ACCGGT 1 cut(s) 464
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviAII CATG 4 cut(s) 334, 454, 553, 652
CviQI GTAC 2 cut(s) 440, 578
DdeI CTNAG 1 cut(s) 222
DpnI GATC 2 cut(s) 41, 264
DpnII GATC 2 cut(s) 39, 262
EaeI YGGCCR 2 cut(s) 49, 270
EagI CGGCCG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 617
Eco52I CGGCCG 1 cut(s) 270
Eco57I CTGAAG 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 306
FaeI CATG 4 cut(s) 337, 457, 556, 655
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 333, 453, 552, 651
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 3 cut(s) 316, 584, 722
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 1 cut(s) 170
GluI GCNGC 1 cut(s) 118
HaeIII GGCC 2 cut(s) 51, 272
HapII CCGG 3 cut(s) 143, 382, 465
Hin1II CATG 4 cut(s) 337, 457, 556, 655
HindIII AAGCTT 2 cut(s) 498, 720
HinfI GANTC 5 cut(s) 18, 204, 233, 599, 736
HpaII CCGG 3 cut(s) 143, 382, 465
HphI GGTGA 3 cut(s) 268, 296, 454
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 4 cut(s) 99, 156, 566, 604
Hpy188III TCNNGA 2 cut(s) 15, 470
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 97, 211
HpyCH4III ACNGT 1 cut(s) 675
HpyCH4V TGCA 4 cut(s) 76, 329, 539, 718
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 4 cut(s) 337, 457, 556, 655
Kzo9I GATC 2 cut(s) 39, 262
LmnI GCTCC 1 cut(s) 361
LweI GCATC 2 cut(s) 338, 435
MaeI CTAG 1 cut(s) 170
MaeIII GTNAC 1 cut(s) 517
MalI GATC 2 cut(s) 41, 264
MbiI CCGCTC 1 cut(s) 371
MboI GATC 2 cut(s) 39, 262
MboII GAAGA 6 cut(s) 18, 48, 51, 169, 324, 485
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 1 cut(s) 533
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 2 cut(s) 210, 339
MslI CAYNNNNRTG 1 cut(s) 324
MspI CCGG 3 cut(s) 143, 382, 465
MspR9I CCNGG 3 cut(s) 143, 144, 308
MvaI CCWGG 1 cut(s) 308
NciI CCSGG 2 cut(s) 143, 144
NdeII GATC 2 cut(s) 39, 262
NlaIII CATG 4 cut(s) 337, 457, 556, 655
NmeAIII GCCGAG 2 cut(s) 248, 298
PfeI GAWTC 4 cut(s) 18, 204, 233, 736
PinAI ACCGGT 1 cut(s) 464
PkrI GCNGC 1 cut(s) 119
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
RsaI GTAC 2 cut(s) 441, 579
RsaNI GTAC 2 cut(s) 440, 578
RseI CAYNNNNRTG 1 cut(s) 324
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 2 cut(s) 39, 262
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 3 cut(s) 143, 144, 308
SduI GDGCHC 1 cut(s) 78
SfaNI GCATC 2 cut(s) 338, 435
SmaI CCCGGG 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 435, 495
SmoI CTYRAG 2 cut(s) 435, 495
Sse9I AATT 1 cut(s) 533
SsiI CCGC 3 cut(s) 118, 288, 371
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 3 cut(s) 141, 142, 306
TaaI ACNGT 1 cut(s) 675
TaqI TCGA 3 cut(s) 38, 188, 669
TasI AATT 1 cut(s) 533
TatI WGTACW 1 cut(s) 439
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 4 cut(s) 18, 204, 233, 736
TscAI CASTG 1 cut(s) 601
TspDTI ATGAA 1 cut(s) 350
TspMI CCCGGG 1 cut(s) 142
TspRI CASTG 1 cut(s) 601
VneI GTGCAC 1 cut(s) 74
XapI RAATTY 1 cut(s) 533
XmaI CCCGGG 1 cut(s) 142
XspI CTAG 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.