Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

5813537 .. 5817669

Gene Structure 4133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g09990.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGAACAGGGCCAAGAAGCGAGGCGGCGGCGGCGGCGGAGGAGGCGGAGGCAGAGGCAGAGGCTCAGGTCCCAAGGGAGCTAAAAAGGGAAAGAGGCAGCCCTCCTTCAAAGAAGACCCGTTTTTCACCAACGAATCGAAGAAGCGGCTCAAATACGACGAGGACATTCGCAGCGACGAGTCGTCCGATGAAGAAGACCGAAACGACGCCGTCCTCGGTAGCGGCGACGAGGAGTTGGAGGCGGCGGAGGAGATCATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTGGGAGAGCATGAGAGGAAGAAGAAAGAGGATGTGGATGACGATAGCGATGACGACAGCGAGAAGGAAGGGGAGAGAGACTCGCTTGTGGCTAAGGTCTTGCAGCAGCAGCAGCTCCAGGACAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCTGAAGCTACTGATGAATTTCGGATCTTAGTGAAGCATCGCCAATCTGTCACCGCGGTTGTTCTCTCTGAGGATGACTTGAGAGGTTTTTCAGCATCTAAGGATGGCACGATTTTGCATTGGGATGTAGATAGTGGGAAAAAAGAGAAGTATCAGTGGCCCAGCGACGAAGTATTAAAGTCCCATGGGGTCAAGGATCCACTAGGTCAAGCTACAAAGCATAGCAAACAAATGTTAGCCCTTGCAGTTAGTTCTGATGGCCGGTATTTGGCTAGTGGAGGATTAGATCGCCATGTTCATTTGTGGGATACTCGTACCAGACAACATGTTCAGGCTTTTCCAGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCGGAACTCTTTTCTGGTTCATATGATCGAACAATTAAGATATGGAATGCAGAAGATAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCAGAGGAGTCACGTTTAGTTTTCCGTGCCCCTGCATGCTCATTGGAGTGTTGCTCTTTTATTAGCAATGATGAATTTTTATCCGGCTCTGATGATGGAAGTGTTGAGCTCTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTGTATTGACTGCACACAAAGATGTTGAACAAAAGGATGGTGATAGAGTCCCCAATGGTCACATAGAAAGCTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGAAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATCAAGCCCTTACACAATCTCCCCGTGGTTGGATTTGTTAATTCTTTAGCTATTGCGAAATCTGGAGGCTTTCTAGTCGCAGGAGTTGGAAAGGAACCTCGACTAGGAAGGTGGGGACTTATCCCAACTGCTCAGAATGGAGTCGCAATCCACTCCCTGAAGCTTTCAGCAGATACAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

526

Amino Acids

58.02

Weight (kDa)

6.78

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	161 - 384	7.1e-19	CDC20/Fizzy WD40 domain
WDR55	PF24796	163 - 397	2e-15	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	192 - 342	6.6e-07	WDR36/Utp21 first beta-propeller
NBCH_WD40	PF20426	204 - 328	1.7e-06	Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd	PF25047	212 - 342	1.5e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	226 - 315	5e-17	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	229 - 345	1.9e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	230 - 349	3.1e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	231 - 390	1.6e-23	WDR5 beta-propeller domain
WD40	PF00400	231 - 262	3.4e-06	WD domain, G-beta repeat
Beta-prop_EML	PF23409	231 - 399	7.8e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	232 - 346	2.4e-23	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	232 - 337	1.6e-06	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	233 - 386	2.1e-11	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_1st	PF24780	234 - 309	5.8e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	235 - 349	4.8e-15	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	235 - 384	4.2e-18	Prp19 WD40 domain
WD40	PF00400	268 - 304	2.3e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	275 - 348	6.2e-08	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	277 - 368	5.9e-06	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	453
AccII	CGCG	1 cut(s)	533
AclWI	GGATC	4 cut(s)	509, 668, 681, 1304
AcoI	YGGCCR	2 cut(s)	439, 736
AcsI	RAATTY	2 cut(s)	494, 1112
AcuI	CTGAAG	3 cut(s)	326, 501, 1574
AcyI	GRCGYC	1 cut(s)	207
AfaI	GTAC	1 cut(s)	793
AfiI	CCNNNNNNNGG	3 cut(s)	745, 1424, 1499
AflIII	ACRYGT	1 cut(s)	802
AgsI	TTSAA	4 cut(s)	109, 1033, 1226, 1379
AhdI	GACNNNNNGTC	1 cut(s)	181
AjnI	CCWGG	2 cut(s)	429, 817
AleI	CACNNNNGTG	1 cut(s)	954
Alw21I	GWGCWC	1 cut(s)	1149
Alw26I	GTCTC	2 cut(s)	257, 384
AlwI	GGATC	4 cut(s)	509, 668, 681, 1304
AlwNI	CAGNNNCTG	1 cut(s)	1346
Ama87I	CYCGRG	1 cut(s)	269
AoxI	GGCC	4 cut(s)	9, 439, 635, 736
ApeKI	GCWGC	7 cut(s)	97, 171, 415, 418, 421, 424, 1159
ApoI	RAATTY	2 cut(s)	494, 1112
AspS9I	GGNCC	5 cut(s)	9, 68, 636, 830, 1152
AsuC2I	CCSGG	2 cut(s)	270, 271
AsuHPI	GGTGA	5 cut(s)	118, 520, 941, 968, 1250
AvaI	CYCGRG	1 cut(s)	269
AvaII	GGWCC	3 cut(s)	68, 830, 1152
BaeGI	GKGCMC	1 cut(s)	1069
BamHI	GGATCC	1 cut(s)	673
BanII	GRGCYC	1 cut(s)	1149
BbsI	GAAGAC	2 cut(s)	120, 201
Bbv12I	GWGCWC	1 cut(s)	1149
BbvCI	CCTCAGC	1 cut(s)	284
BbvI	GCAGC	7 cut(s)	109, 183, 427, 430, 433, 436, 1146
BccI	CCATC	5 cut(s)	575, 728, 1127, 1229, 1403
BceAI	ACGGC	2 cut(s)	194, 1007
BciT130I	CCWGG	2 cut(s)	431, 819
BciVI	GTATCC	1 cut(s)	778
BcnI	CCSGG	2 cut(s)	270, 271
BcoDI	GTCTC	2 cut(s)	257, 384
BfaI	CTAG	5 cut(s)	465, 680, 750, 1469, 1499
BfuI	GTATCC	1 cut(s)	778
Bme1390I	CCNGG	4 cut(s)	270, 271, 431, 819
Bme18I	GGWCC	3 cut(s)	68, 830, 1152
BmeRI	GACNNNNNGTC	1 cut(s)	181
BmeT110I	CYCGRG	1 cut(s)	269
BmgT120I	GGNCC	5 cut(s)	9, 68, 636, 830, 1152
BmiI	GGNNCC	5 cut(s)	70, 675, 831, 1038, 1491
BmrFI	CCNGG	4 cut(s)	270, 271, 431, 819
BmsI	GCATC	3 cut(s)	523, 581, 1346
BpiI	GAAGAC	2 cut(s)	120, 201
BplI	GAGNNNNNCTC	4 cut(s)	377, 409, 1076, 1108
BpmI	CTGGAG	2 cut(s)	413, 1479
Bpu10I	CCTNAGC	3 cut(s)	64, 284, 405
BpuEI	CTTGAG	1 cut(s)	577
BpuMI	CCSGG	2 cut(s)	270, 271
BsaHI	GRCGYC	1 cut(s)	207
BsaJI	CCNNGG	8 cut(s)	72, 214, 269, 270, 531, 661, 952, 1419
BsaXI	ACNNNNNCTCC	2 cut(s)	1398, 1428
Bsc4I	CCNNNNNNNGG	3 cut(s)	745, 1424, 1499
Bse118I	RCCGGY	1 cut(s)	738
Bse3DI	GCAATG	3 cut(s)	456, 1111, 1396
BseBI	CCWGG	2 cut(s)	431, 819
BseDI	CCNNGG	8 cut(s)	72, 214, 269, 270, 531, 661, 952, 1419
BseGI	GGATG	7 cut(s)	349, 355, 556, 586, 607, 1195, 1240
BseLI	CCNNNNNNNGG	3 cut(s)	745, 1424, 1499
BseMI	GCAATG	3 cut(s)	456, 1111, 1396
BseMII	CTCAG	4 cut(s)	78, 275, 537, 1541
BseRI	GAGGAG	5 cut(s)	54, 245, 263, 275, 1058
BseSI	GKGCMC	1 cut(s)	1069
BseX3I	CGGCCG	1 cut(s)	439
BseXI	GCAGC	7 cut(s)	109, 183, 427, 430, 433, 436, 1146
BseYI	CCCAGC	2 cut(s)	313, 638
BsgI	GTGCAG	1 cut(s)	1194
Bsh1236I	CGCG	1 cut(s)	533
Bsh1285I	CGRYCG	1 cut(s)	442
BshFI	GGCC	4 cut(s)	11, 441, 637, 738
BsiEI	CGRYCG	1 cut(s)	442
BsiHKAI	GWGCWC	1 cut(s)	1149
BsiHKCI	CYCGRG	1 cut(s)	269
BsiSI	CCGG	3 cut(s)	270, 739, 1122
BslFI	GGGAC	5 cut(s)	54, 643, 843, 1232, 1524
BslI	CCNNNNNNNGG	3 cut(s)	745, 1424, 1499
BsmAI	GTCTC	2 cut(s)	257, 384
BsmFI	GGGAC	5 cut(s)	54, 643, 843, 1232, 1524
BsmI	GAATGC	2 cut(s)	916, 1195
BsnI	GGCC	4 cut(s)	11, 441, 637, 738
BsoBI	CYCGRG	1 cut(s)	269
Bsp1286I	GDGCHC	2 cut(s)	1069, 1149
Bsp143I	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
Bsp19I	CCATGG	1 cut(s)	661
BspANI	GGCC	4 cut(s)	11, 441, 637, 738
BspCNI	CTCAG	4 cut(s)	77, 276, 538, 1540
BspFNI	CGCG	1 cut(s)	533
BspLI	GGNNCC	5 cut(s)	70, 675, 831, 1038, 1491
BspPI	GGATC	4 cut(s)	509, 668, 681, 1304
BsrBI	CCGCTC	1 cut(s)	453
BsrDI	GCAATG	3 cut(s)	456, 1111, 1396
BsrFI	RCCGGY	1 cut(s)	738
BssAI	RCCGGY	1 cut(s)	738
BssECI	CCNNGG	8 cut(s)	72, 214, 269, 270, 531, 661, 952, 1419
BssMI	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
BssNI	GRCGYC	1 cut(s)	207
BssT1I	CCWWGG	3 cut(s)	72, 661, 952
Bst2UI	CCWGG	2 cut(s)	431, 819
Bst4CI	ACNGT	1 cut(s)	1003
Bst6I	CTCTTC	1 cut(s)	302
BstACI	GRCGYC	1 cut(s)	207
BstC8I	GCNNGC	1 cut(s)	1075
BstDEI	CTNAG	7 cut(s)	64, 284, 405, 505, 546, 576, 1527
BstDSI	CCRYGG	3 cut(s)	531, 661, 1419
BstEII	GGTNACC	1 cut(s)	947
BstENI	CCTNNNNNAGG	1 cut(s)	1497
BstF5I	GGATG	7 cut(s)	349, 355, 556, 586, 607, 1195, 1240
BstFNI	CGCG	1 cut(s)	533
BstKTI	GATC	7 cut(s)	255, 504, 676, 766, 892, 1015, 1299
BstMAI	GTCTC	2 cut(s)	257, 384
BstMBI	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
BstMCI	CGRYCG	1 cut(s)	442
BstNI	CCWGG	2 cut(s)	431, 819
BstNSI	RCATGY	2 cut(s)	806, 1077
BstPI	GGTNACC	1 cut(s)	947
BstSCI	CCNGG	4 cut(s)	268, 269, 429, 817
BstSLI	GKGCMC	1 cut(s)	1069
BstUI	CGCG	1 cut(s)	533
BstV1I	GCAGC	7 cut(s)	109, 183, 427, 430, 433, 436, 1146
BstV2I	GAAGAC	2 cut(s)	120, 201
BstX2I	RGATCY	2 cut(s)	501, 673
BstYI	RGATCY	2 cut(s)	501, 673
BstZI	CGGCCG	1 cut(s)	439
BsuI	GTATCC	1 cut(s)	778
BsuRI	GGCC	4 cut(s)	11, 441, 637, 738
BtgI	CCRYGG	3 cut(s)	531, 661, 1419
BtgZI	GCGATG	2 cut(s)	375, 500
BtsCI	GGATG	7 cut(s)	349, 355, 556, 586, 607, 1195, 1240
BtsI	GCAGTG	1 cut(s)	1330
BtsIMutI	CAGTG	2 cut(s)	638, 1330
Cac8I	GCNNGC	1 cut(s)	1075
CaiI	CAGNNNCTG	1 cut(s)	1346
CciNI	GCGGCCGC	1 cut(s)	439
Cfr10I	RCCGGY	1 cut(s)	738
Cfr13I	GGNCC	5 cut(s)	9, 68, 636, 830, 1152
Cfr42I	CCGCGG	1 cut(s)	534
Cfr9I	CCCGGG	1 cut(s)	269
CseI	GACGC	1 cut(s)	215
Csp6I	GTAC	1 cut(s)	792
CviAII	CATG	9 cut(s)	323, 662, 770, 803, 840, 1010, 1021, 1074, 1156
CviQI	GTAC	1 cut(s)	792
DdeI	CTNAG	7 cut(s)	64, 284, 405, 505, 546, 576, 1527
DpnI	GATC	7 cut(s)	254, 503, 675, 765, 891, 1014, 1298
DpnII	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
DriI	GACNNNNNGTC	1 cut(s)	181
EaeI	YGGCCR	2 cut(s)	439, 736
EagI	CGGCCG	1 cut(s)	439
Eam1104I	CTCTTC	1 cut(s)	302
Eam1105I	GACNNNNNGTC	1 cut(s)	181
EarI	CTCTTC	1 cut(s)	302
EciI	GGCGGA	4 cut(s)	51, 60, 260, 437
Ecl136II	GAGCTC	1 cut(s)	1147
EclXI	CGGCCG	1 cut(s)	439
Eco130I	CCWWGG	3 cut(s)	72, 661, 952
Eco24I	GRGCYC	1 cut(s)	1149
Eco47I	GGWCC	3 cut(s)	68, 830, 1152
Eco52I	CGGCCG	1 cut(s)	439
Eco53kI	GAGCTC	1 cut(s)	1147
Eco57I	CTGAAG	3 cut(s)	326, 501, 1574
Eco88I	CYCGRG	1 cut(s)	269
Eco91I	GGTNACC	1 cut(s)	947
EcoICRI	GAGCTC	1 cut(s)	1147
EcoNI	CCTNNNNNAGG	1 cut(s)	1497
EcoO109I	RGGNCCY	2 cut(s)	68, 830
EcoO65I	GGTNACC	1 cut(s)	947
EcoRII	CCWGG	2 cut(s)	429, 817
EcoT14I	CCWWGG	3 cut(s)	72, 661, 952
EcoT22I	ATGCAT	1 cut(s)	1181
EcoT38I	GRGCYC	1 cut(s)	1149
ErhI	CCWWGG	3 cut(s)	72, 661, 952
FaeI	CATG	9 cut(s)	326, 665, 773, 806, 843, 1013, 1024, 1077, 1159
FalI	AAGNNNNNCTT	2 cut(s)	978, 1010
FaqI	GGGAC	5 cut(s)	54, 643, 843, 1232, 1524
FatI	CATG	9 cut(s)	322, 661, 769, 802, 839, 1009, 1020, 1073, 1155
FauNDI	CATATG	1 cut(s)	886
FokI	GGATG	7 cut(s)	356, 362, 563, 593, 614, 1182, 1247
FriOI	GRGCYC	1 cut(s)	1149
FspBI	CTAG	5 cut(s)	465, 680, 750, 1469, 1499
GsaI	CCCAGC	2 cut(s)	317, 642
GsuI	CTGGAG	2 cut(s)	413, 1479
HaeIII	GGCC	4 cut(s)	11, 441, 637, 738
HapII	CCGG	3 cut(s)	270, 739, 1122
HgaI	GACGC	1 cut(s)	215
Hin1I	GRCGYC	1 cut(s)	207
Hin1II	CATG	9 cut(s)	326, 665, 773, 806, 843, 1013, 1024, 1077, 1159
HindIII	AAGCTT	1 cut(s)	1556
HinfI	GANTC	7 cut(s)	134, 179, 265, 392, 1046, 1245, 1536
HpaII	CCGG	3 cut(s)	270, 739, 1122
HphI	GGTGA	5 cut(s)	118, 520, 941, 968, 1250
Hpy188I	TCNGA	9 cut(s)	187, 306, 474, 501, 547, 733, 868, 1129, 1530
Hpy188III	TCNNGA	4 cut(s)	1040, 1150, 1341, 1458
Hpy99I	CGWCG	5 cut(s)	161, 179, 209, 230, 647
HpyAV	CCTTC	5 cut(s)	115, 289, 370, 374, 1497
HpyCH4III	ACNGT	1 cut(s)	1003
HpyCH4IV	ACGT	1 cut(s)	1051
HpyCH4V	TGCA	7 cut(s)	415, 595, 722, 914, 1073, 1179, 1211
HpyF3I	CTNAG	7 cut(s)	64, 284, 405, 505, 546, 576, 1527
HpySE526I	ACGT	1 cut(s)	1051
Hsp92I	GRCGYC	1 cut(s)	207
Hsp92II	CATG	9 cut(s)	326, 665, 773, 806, 843, 1013, 1024, 1077, 1159
KspI	CCGCGG	1 cut(s)	534
Kzo9I	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
LmnI	GCTCC	4 cut(s)	77, 432, 458, 1343
Lsp1109I	GCAGC	7 cut(s)	109, 183, 427, 430, 433, 436, 1146
LweI	GCATC	3 cut(s)	523, 581, 1346
MaeI	CTAG	5 cut(s)	465, 680, 750, 1469, 1499
MaeII	ACGT	1 cut(s)	1051
MaeIII	GTNAC	7 cut(s)	526, 821, 947, 1047, 1256, 1306, 1325
MalI	GATC	7 cut(s)	254, 503, 675, 765, 891, 1014, 1298
MbiI	CCGCTC	1 cut(s)	453
MboI	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
MboII	GAAGA	9 cut(s)	125, 151, 203, 206, 289, 319, 343, 346, 929
MflI	RGATCY	2 cut(s)	501, 673
MhlI	GDGCHC	2 cut(s)	1069, 1149
MluCI	AATT	5 cut(s)	494, 897, 1112, 1435, 1573
MlyI	GAGTC	6 cut(s)	188, 259, 386, 1055, 1254, 1545
MmeI	TCCRAC	4 cut(s)	216, 985, 1405, 1462
Mph1103I	ATGCAT	1 cut(s)	1181
MseI	TTAA	5 cut(s)	653, 845, 900, 1185, 1434
MslI	CAYNNNNRTG	4 cut(s)	600, 954, 1084, 1218
MspA1I	CMGCKG	2 cut(s)	438, 533
MspI	CCGG	3 cut(s)	270, 739, 1122
MspR9I	CCNGG	4 cut(s)	270, 271, 431, 819
Mva1269I	GAATGC	2 cut(s)	916, 1195
MvaI	CCWGG	2 cut(s)	431, 819
MvnI	CGCG	1 cut(s)	533
NciI	CCSGG	2 cut(s)	270, 271
NcoI	CCATGG	1 cut(s)	661
NdeI	CATATG	1 cut(s)	886
NdeII	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
NlaIII	CATG	9 cut(s)	326, 665, 773, 806, 843, 1013, 1024, 1077, 1159
NlaIV	GGNNCC	5 cut(s)	70, 675, 831, 1038, 1491
NmuCI	GTSAC	7 cut(s)	526, 821, 947, 1047, 1256, 1306, 1325
NotI	GCGGCCGC	1 cut(s)	439
NsiI	ATGCAT	1 cut(s)	1181
NspI	RCATGY	2 cut(s)	806, 1077
OliI	CACNNNNGTG	1 cut(s)	954
PaeI	GCATGC	1 cut(s)	1077
PciI	ACATGT	1 cut(s)	802
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 222
PctI	GAATGC	2 cut(s)	916, 1195
PfeI	GAWTC	1 cut(s)	134
PflFI	GACNNNGTC	1 cut(s)	209
PfoI	TCCNGGA	1 cut(s)	429
PleI	GAGTC	6 cut(s)	187, 259, 386, 1054, 1253, 1544
PpsI	GAGTC	6 cut(s)	187, 259, 386, 1054, 1253, 1544
PpuMI	RGGWCCY	2 cut(s)	68, 830
PscI	ACATGT	1 cut(s)	802
Psp124BI	GAGCTC	1 cut(s)	1149
Psp5II	RGGWCCY	2 cut(s)	68, 830
Psp6I	CCWGG	2 cut(s)	429, 817
PspEI	GGTNACC	1 cut(s)	947
PspFI	CCCAGC	2 cut(s)	313, 638
PspGI	CCWGG	2 cut(s)	429, 817
PspN4I	GGNNCC	5 cut(s)	70, 675, 831, 1038, 1491
PspPI	GGNCC	5 cut(s)	9, 68, 636, 830, 1152
PspPPI	RGGWCCY	2 cut(s)	68, 830
PstNI	CAGNNNCTG	1 cut(s)	1346
PsuI	RGATCY	2 cut(s)	501, 673
PsyI	GACNNNGTC	1 cut(s)	209
RsaI	GTAC	1 cut(s)	793
RsaNI	GTAC	1 cut(s)	792
RseI	CAYNNNNRTG	4 cut(s)	600, 954, 1084, 1218
SacI	GAGCTC	1 cut(s)	1149
SacII	CCGCGG	1 cut(s)	534
SaqAI	TTAA	5 cut(s)	653, 845, 900, 1185, 1434
Sau3AI	GATC	7 cut(s)	252, 501, 673, 763, 889, 1012, 1296
Sau96I	GGNCC	5 cut(s)	9, 68, 636, 830, 1152
SchI	GAGTC	6 cut(s)	188, 259, 386, 1055, 1254, 1545
ScrFI	CCNGG	4 cut(s)	270, 271, 431, 819
SduI	GDGCHC	2 cut(s)	1069, 1149
SfaNI	GCATC	3 cut(s)	523, 581, 1346
Sfr303I	CCGCGG	1 cut(s)	534
SgrBI	CCGCGG	1 cut(s)	534
SinI	GGWCC	3 cut(s)	68, 830, 1152
SmaI	CCCGGG	1 cut(s)	271
SmiMI	CAYNNNNRTG	4 cut(s)	600, 954, 1084, 1218
SmlI	CTYRAG	1 cut(s)	556
SmoI	CTYRAG	1 cut(s)	556
SphI	GCATGC	1 cut(s)	1077
Sse9I	AATT	5 cut(s)	494, 897, 1112, 1435, 1573
SspMI	CTAG	5 cut(s)	465, 680, 750, 1469, 1499
SstI	GAGCTC	1 cut(s)	1149
StyD4I	CCNGG	4 cut(s)	268, 269, 429, 817
StyI	CCWWGG	3 cut(s)	72, 661, 952
TaaI	ACNGT	1 cut(s)	1003
TaiI	ACGT	1 cut(s)	1054
TaqI	TCGA	5 cut(s)	137, 892, 1015, 1318, 1495
TaqII	GACCGA	1 cut(s)	213
TasI	AATT	5 cut(s)	494, 897, 1112, 1435, 1573
TfiI	GAWTC	1 cut(s)	134
Tru1I	TTAA	5 cut(s)	653, 845, 900, 1185, 1434
Tru9I	TTAA	5 cut(s)	653, 845, 900, 1185, 1434
TscAI	CASTG	2 cut(s)	638, 1330
TseFI	GTSAC	7 cut(s)	526, 821, 947, 1047, 1256, 1306, 1325
TseI	GCWGC	7 cut(s)	97, 171, 415, 418, 421, 424, 1159
Tsp45I	GTSAC	7 cut(s)	526, 821, 947, 1047, 1256, 1306, 1325
TspDTI	ATGAA	7 cut(s)	17, 204, 507, 764, 873, 1125, 1269
TspGWI	ACGGA	2 cut(s)	997, 1052
TspMI	CCCGGG	1 cut(s)	269
TspRI	CASTG	2 cut(s)	638, 1330
Tth111I	GACNNNGTC	1 cut(s)	209
VpaK11BI	GGWCC	3 cut(s)	68, 830, 1152
XagI	CCTNNNNNAGG	1 cut(s)	1497
XapI	RAATTY	2 cut(s)	494, 1112
XceI	RCATGY	2 cut(s)	806, 1077
XmaI	CCCGGG	1 cut(s)	269
XspI	CTAG	5 cut(s)	465, 680, 750, 1469, 1499
Zsp2I	ATGCAT	1 cut(s)	1181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.