Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

10976115 .. 10980072

Gene Structure 3958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29629

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGAACAGAAACAAGAAGCGAGGCGGCGGCGGCGGGGGAGGCTCAGCTCCCAAGGGCGGAAGAGGAGGAGCCAAAAAAGGCAAGAGGCAGTCGTTCAAGGAAGACCCATTTTTCACCAACGAATCCAAGAAGCGGCTCAAATACGACGAGGACATTCGGAGCGACGAGTCGTCCGACGATGAACGAAACGACGCCGTCGGTAGTGGCGAGGAGGAGGAGGAGGAGGCGGCTGAGGAGATTATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTTGGAGAGCATGAGAGGAAGAAGAAGGAAGACGAGGATGACGATAGCGATGACGACAGCGAGAAGGAAGGCGACAGAGACTCGCTTGTCGCCAAGGTTTTGCAGCAGCAGCAGCTCCAGGACAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCAGAAGCTACTGATGAGTTTCGGATATTAGTGAAGCATCGCCAATCTGTCACTGCAGTGGTTCTCTCTGATGATGATGGCAGAGGTTTTTCAGCATCTAAGGACGGCGCGATTTTGCATTGGGATGTAGATAGTGGGAAGAAAGAGAAGTATCAGTGGCCCAGTGACGAAGTATTGAAGTCCCACGGGGTCAAGGATCCACTAGGTAGAGCTACAAAGCATAGCAAACAAATGTTAGCCCTTGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATATTCATTTGTGGGATACTCGTACCAGACAGCATGTTCAGGCTTTTCCGGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCAGAACTCTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGCAGAAGACAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCAGAGGAGTCGCGTTTAGTATTCCGTGCCCCTGCATGCTCATTGGAGTGTTGTTCTTTTATTAGCAATGATGAATTCTTATCCGGCTCTGATAATGGAAGTGTTGAGCTTTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTTTGTTAGCTGCACACAAAGATGTTGAACAGAAGGATGGTGATAGAGTCCCCAATGGTCACATAGAAAGCTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGTAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATTCAGCCCTTACACAGTCTCCCCGTGGTTGGATTTGTTAATTCTTTAGCTATTGCAAAATCTGGAGGCTTTCTAGTTGCAGGAGTCGGAAAGGAACCGCGTCTGGGAAGGTGGGGACTTATCCCAGCTGCTCAGAATGGAGTTGCAATCCACACCCTTAAGCTTTCGTCAGATACAAATAAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

522

Amino Acids

57.77

Weight (kDa)

6.62

Isoelectric Point (pI)

51.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WDR55	PF24796	158 - 338	5.3e-11	WDR55
WD40_CDC20-Fz	PF24807	158 - 343	7.1e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	187 - 337	1e-06	WDR36/Utp21 first beta-propeller
NBCH_WD40	PF20426	199 - 323	2.1e-06	Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd	PF25047	207 - 336	1.8e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	222 - 281	2.1e-10	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	224 - 339	3.1e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	226 - 257	3.3e-06	WD domain, G-beta repeat
Beta-prop_EML	PF23409	226 - 394	5.4e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	226 - 386	4.4e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	227 - 341	2.5e-23	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	228 - 381	1e-10	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	229 - 343	6.9e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	229 - 332	1.8e-06	WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	229 - 304	8.1e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	230 - 344	3.1e-15	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	230 - 388	4e-19	Prp19 WD40 domain
WD40	PF00400	263 - 299	2.4e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	270 - 343	4.8e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	383
AccBSI	CCGCTC	1 cut(s)	438
AccII	CGCG	3 cut(s)	572, 1036, 1480
AclWI	GGATC	3 cut(s)	653, 666, 1289
AcoI	YGGCCR	1 cut(s)	424
AcsI	RAATTY	1 cut(s)	1097
AcuI	CTGAAG	1 cut(s)	311
AcyI	GRCGYC	1 cut(s)	192
AfaI	GTAC	1 cut(s)	778
AfiI	CCNNNNNNNGG	4 cut(s)	56, 132, 1409, 1484
AflII	CTTAAG	1 cut(s)	1538
AgsI	TTSAA	5 cut(s)	97, 640, 1018, 1211, 1364
AhdI	GACNNNNNGTC	1 cut(s)	169
AjnI	CCWGG	1 cut(s)	414
AleI	CACNNNNGTG	2 cut(s)	518, 939
Alw26I	GTCTC	3 cut(s)	242, 369, 1403
AlwI	GGATC	3 cut(s)	653, 666, 1289
AlwNI	CAGNNNCTG	1 cut(s)	1331
Ama87I	CYCGRG	1 cut(s)	254
AoxI	GGCC	2 cut(s)	424, 620
ApeKI	GCWGC	7 cut(s)	400, 403, 406, 409, 1144, 1193, 1508
ApoI	RAATTY	1 cut(s)	1097
AspLEI	GCGC	1 cut(s)	572
AspS9I	GGNCC	3 cut(s)	621, 815, 1137
AsuC2I	CCSGG	3 cut(s)	255, 256, 804
AsuHPI	GGTGA	4 cut(s)	106, 926, 953, 1235
AvaI	CYCGRG	1 cut(s)	254
AvaII	GGWCC	2 cut(s)	815, 1137
BaeGI	GKGCMC	1 cut(s)	1054
BamHI	GGATCC	1 cut(s)	658
BbsI	GAAGAC	3 cut(s)	108, 333, 909
BbvCI	CCTCAGC	2 cut(s)	231, 269
BbvI	GCAGC	7 cut(s)	412, 415, 418, 421, 1131, 1180, 1495
BccI	CCATC	3 cut(s)	533, 713, 1214
BceAI	ACGGC	3 cut(s)	179, 583, 992
BciT130I	CCWGG	1 cut(s)	416
BciVI	GTATCC	1 cut(s)	763
BcnI	CCSGG	3 cut(s)	255, 256, 804
BcoDI	GTCTC	3 cut(s)	242, 369, 1403
BfaI	CTAG	3 cut(s)	450, 665, 1454
BfmI	CTRYAG	1 cut(s)	516
BfrI	CTTAAG	1 cut(s)	1538
BfuI	GTATCC	1 cut(s)	763
BlpI	GCTNAGC	1 cut(s)	43
Bme1390I	CCNGG	4 cut(s)	255, 256, 416, 804
Bme18I	GGWCC	2 cut(s)	815, 1137
BmeRI	GACNNNNNGTC	1 cut(s)	169
BmeT110I	CYCGRG	1 cut(s)	254
BmgT120I	GGNCC	3 cut(s)	621, 815, 1137
BmiI	GGNNCC	5 cut(s)	70, 660, 816, 1023, 1476
BmrFI	CCNGG	4 cut(s)	255, 256, 416, 804
BmrI	ACTGGG	1 cut(s)	618
BmsI	GCATC	3 cut(s)	508, 566, 1331
BmuI	ACTGGG	1 cut(s)	618
BpiI	GAAGAC	3 cut(s)	108, 333, 909
BpmI	CTGGAG	2 cut(s)	398, 1464
Bpu10I	CCTNAGC	2 cut(s)	231, 269
Bpu1102I	GCTNAGC	1 cut(s)	43
BpuMI	CCSGG	3 cut(s)	255, 256, 804
BsaHI	GRCGYC	1 cut(s)	192
BsaJI	CCNNGG	7 cut(s)	51, 254, 255, 390, 646, 937, 1404
BsaXI	ACNNNNNCTCC	2 cut(s)	1383, 1413
Bsc4I	CCNNNNNNNGG	4 cut(s)	56, 132, 1409, 1484
Bse1I	ACTGG	1 cut(s)	624
Bse3DI	GCAATG	3 cut(s)	441, 1096, 1381
BseBI	CCWGG	1 cut(s)	416
BseDI	CCNNGG	7 cut(s)	51, 254, 255, 390, 646, 937, 1404
BseGI	GGATG	4 cut(s)	340, 592, 1180, 1225
BseLI	CCNNNNNNNGG	4 cut(s)	56, 132, 1409, 1484
BseMI	GCAATG	3 cut(s)	441, 1096, 1381
BseMII	CTCAG	4 cut(s)	57, 222, 260, 1526
BseNI	ACTGG	1 cut(s)	624
BseSI	GKGCMC	1 cut(s)	1054
BseX3I	CGGCCG	1 cut(s)	424
BseXI	GCAGC	7 cut(s)	412, 415, 418, 421, 1131, 1180, 1495
BseYI	CCCAGC	1 cut(s)	1504
BsgI	GTGCAG	1 cut(s)	1179
Bsh1236I	CGCG	3 cut(s)	572, 1036, 1480
Bsh1285I	CGRYCG	1 cut(s)	427
BshFI	GGCC	2 cut(s)	426, 622
BsiEI	CGRYCG	1 cut(s)	427
BsiHKCI	CYCGRG	1 cut(s)	254
BsiSI	CCGG	3 cut(s)	255, 803, 1107
BslFI	GGGAC	4 cut(s)	628, 828, 1217, 1509
BslI	CCNNNNNNNGG	4 cut(s)	56, 132, 1409, 1484
BsmAI	GTCTC	3 cut(s)	242, 369, 1403
BsmFI	GGGAC	4 cut(s)	628, 828, 1217, 1509
BsmI	GAATGC	2 cut(s)	901, 1180
BsnI	GGCC	2 cut(s)	426, 622
BsoBI	CYCGRG	1 cut(s)	254
Bsp1286I	GDGCHC	1 cut(s)	1054
Bsp143I	GATC	5 cut(s)	658, 748, 874, 997, 1281
Bsp1720I	GCTNAGC	1 cut(s)	43
BspANI	GGCC	2 cut(s)	426, 622
BspCNI	CTCAG	4 cut(s)	56, 223, 261, 1525
BspFNI	CGCG	3 cut(s)	572, 1036, 1480
BspLI	GGNNCC	5 cut(s)	70, 660, 816, 1023, 1476
BspMAI	CTGCAG	1 cut(s)	520
BspPI	GGATC	3 cut(s)	653, 666, 1289
BspTI	CTTAAG	1 cut(s)	1538
BsrBI	CCGCTC	1 cut(s)	438
BsrDI	GCAATG	3 cut(s)	441, 1096, 1381
BsrI	ACTGG	1 cut(s)	624
BssECI	CCNNGG	7 cut(s)	51, 254, 255, 390, 646, 937, 1404
BssMI	GATC	5 cut(s)	658, 748, 874, 997, 1281
BssNI	GRCGYC	1 cut(s)	192
BssT1I	CCWWGG	3 cut(s)	51, 390, 937
Bst2UI	CCWGG	1 cut(s)	416
Bst4CI	ACNGT	3 cut(s)	883, 988, 1397
Bst6I	CTCTTC	2 cut(s)	55, 287
BstACI	GRCGYC	1 cut(s)	192
BstAFI	CTTAAG	1 cut(s)	1538
BstC8I	GCNNGC	1 cut(s)	1060
BstDEI	CTNAG	5 cut(s)	43, 231, 269, 561, 1512
BstDSI	CCRYGG	2 cut(s)	646, 1404
BstEII	GGTNACC	1 cut(s)	932
BstF5I	GGATG	4 cut(s)	340, 592, 1180, 1225
BstFNI	CGCG	3 cut(s)	572, 1036, 1480
BstHHI	GCGC	1 cut(s)	572
BstKTI	GATC	5 cut(s)	661, 751, 877, 1000, 1284
BstMAI	GTCTC	3 cut(s)	242, 369, 1403
BstMBI	GATC	5 cut(s)	658, 748, 874, 997, 1281
BstMCI	CGRYCG	1 cut(s)	427
BstMWI	GCNNNNNNNGC	9 cut(s)	30, 39, 304, 406, 409, 432, 435, 1153, 1328
BstNI	CCWGG	1 cut(s)	416
BstNSI	RCATGY	2 cut(s)	791, 1062
BstPI	GGTNACC	1 cut(s)	932
BstSCI	CCNGG	4 cut(s)	253, 254, 414, 802
BstSFI	CTRYAG	1 cut(s)	516
BstSLI	GKGCMC	1 cut(s)	1054
BstUI	CGCG	3 cut(s)	572, 1036, 1480
BstV1I	GCAGC	7 cut(s)	412, 415, 418, 421, 1131, 1180, 1495
BstV2I	GAAGAC	3 cut(s)	108, 333, 909
BstX2I	RGATCY	1 cut(s)	658
BstYI	RGATCY	1 cut(s)	658
BstZI	CGGCCG	1 cut(s)	424
BsuI	GTATCC	1 cut(s)	763
BsuRI	GGCC	2 cut(s)	426, 622
BtgI	CCRYGG	2 cut(s)	646, 1404
BtgZI	GCGATG	2 cut(s)	360, 485
BtsCI	GGATG	4 cut(s)	340, 592, 1180, 1225
BtsI	GCAGTG	3 cut(s)	513, 525, 1315
BtsIMutI	CAGTG	5 cut(s)	513, 525, 623, 631, 1315
Cac8I	GCNNGC	1 cut(s)	1060
CaiI	CAGNNNCTG	1 cut(s)	1331
CciNI	GCGGCCGC	1 cut(s)	424
CfoI	GCGC	1 cut(s)	572
Cfr13I	GGNCC	3 cut(s)	621, 815, 1137
Cfr9I	CCCGGG	1 cut(s)	254
CseI	GACGC	2 cut(s)	200, 1469
Csp6I	GTAC	1 cut(s)	777
CviAII	CATG	7 cut(s)	308, 788, 825, 995, 1006, 1059, 1141
CviQI	GTAC	1 cut(s)	777
DdeI	CTNAG	5 cut(s)	43, 231, 269, 561, 1512
DpnI	GATC	5 cut(s)	660, 750, 876, 999, 1283
DpnII	GATC	5 cut(s)	658, 748, 874, 997, 1281
DrdI	GACNNNNNNGTC	1 cut(s)	383
DriI	GACNNNNNGTC	1 cut(s)	169
DseDI	GACNNNNNNGTC	1 cut(s)	383
EaeI	YGGCCR	1 cut(s)	424
EagI	CGGCCG	1 cut(s)	424
Eam1104I	CTCTTC	2 cut(s)	55, 287
Eam1105I	GACNNNNNGTC	1 cut(s)	169
EarI	CTCTTC	2 cut(s)	55, 287
EciI	GGCGGA	2 cut(s)	72, 422
EclXI	CGGCCG	1 cut(s)	424
Eco130I	CCWWGG	3 cut(s)	51, 390, 937
Eco47I	GGWCC	2 cut(s)	815, 1137
Eco52I	CGGCCG	1 cut(s)	424
Eco57I	CTGAAG	1 cut(s)	311
Eco88I	CYCGRG	1 cut(s)	254
Eco91I	GGTNACC	1 cut(s)	932
EcoO109I	RGGNCCY	1 cut(s)	815
EcoO65I	GGTNACC	1 cut(s)	932
EcoRI	GAATTC	1 cut(s)	1097
EcoRII	CCWGG	1 cut(s)	414
EcoT14I	CCWWGG	3 cut(s)	51, 390, 937
EcoT22I	ATGCAT	1 cut(s)	1166
ErhI	CCWWGG	3 cut(s)	51, 390, 937
FaeI	CATG	7 cut(s)	311, 791, 828, 998, 1009, 1062, 1144
FalI	AAGNNNNNCTT	2 cut(s)	963, 995
FaqI	GGGAC	4 cut(s)	628, 828, 1217, 1509
FatI	CATG	7 cut(s)	307, 787, 824, 994, 1005, 1058, 1140
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	871
FokI	GGATG	4 cut(s)	347, 599, 1167, 1232
FspBI	CTAG	3 cut(s)	450, 665, 1454
GlaI	GCGC	1 cut(s)	571
GsaI	CCCAGC	1 cut(s)	1508
GsuI	CTGGAG	2 cut(s)	398, 1464
HaeIII	GGCC	2 cut(s)	426, 622
HapII	CCGG	3 cut(s)	255, 803, 1107
HgaI	GACGC	2 cut(s)	200, 1469
HhaI	GCGC	1 cut(s)	572
Hin1I	GRCGYC	1 cut(s)	192
Hin1II	CATG	7 cut(s)	311, 791, 828, 998, 1009, 1062, 1144
Hin6I	GCGC	1 cut(s)	570
HinP1I	GCGC	1 cut(s)	570
HindIII	AAGCTT	2 cut(s)	1541, 1562
HinfI	GANTC	7 cut(s)	122, 167, 250, 377, 1031, 1230, 1464
HpaII	CCGG	3 cut(s)	255, 803, 1107
HphI	GGTGA	4 cut(s)	106, 926, 953, 1235
Hpy188III	TCNNGA	3 cut(s)	1025, 1326, 1443
Hpy99I	CGWCG	5 cut(s)	149, 167, 179, 194, 200
HpyAV	CCTTC	6 cut(s)	274, 316, 355, 359, 1210, 1482
HpyCH4III	ACNGT	3 cut(s)	883, 988, 1397
HpyF10VI	GCNNNNNNNGC	9 cut(s)	30, 39, 304, 406, 409, 432, 435, 1153, 1328
HpyF3I	CTNAG	5 cut(s)	43, 231, 269, 561, 1512
Hsp92I	GRCGYC	1 cut(s)	192
Hsp92II	CATG	7 cut(s)	311, 791, 828, 998, 1009, 1062, 1144
HspAI	GCGC	1 cut(s)	570
Kzo9I	GATC	5 cut(s)	658, 748, 874, 997, 1281
LmnI	GCTCC	6 cut(s)	52, 68, 159, 417, 443, 1328
Lsp1109I	GCAGC	7 cut(s)	412, 415, 418, 421, 1131, 1180, 1495
LweI	GCATC	3 cut(s)	508, 566, 1331
MaeI	CTAG	3 cut(s)	450, 665, 1454
MaeIII	GTNAC	7 cut(s)	511, 626, 806, 932, 1241, 1291, 1310
MalI	GATC	5 cut(s)	660, 750, 876, 999, 1283
MbiI	CCGCTC	1 cut(s)	438
MboI	GATC	5 cut(s)	658, 748, 874, 997, 1281
MboII	GAAGA	9 cut(s)	72, 113, 274, 304, 328, 331, 338, 613, 914
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	1 cut(s)	1054
MluCI	AATT	2 cut(s)	1097, 1420
MlyI	GAGTC	6 cut(s)	176, 244, 371, 1040, 1239, 1473
MmeI	TCCRAC	4 cut(s)	198, 970, 1390, 1447
Mph1103I	ATGCAT	1 cut(s)	1166
MseI	TTAA	5 cut(s)	830, 885, 1170, 1419, 1539
MslI	CAYNNNNRTG	5 cut(s)	518, 585, 939, 1069, 1203
MspA1I	CMGCKG	2 cut(s)	423, 1508
MspCI	CTTAAG	1 cut(s)	1538
MspI	CCGG	3 cut(s)	255, 803, 1107
MspR9I	CCNGG	4 cut(s)	255, 256, 416, 804
Mva1269I	GAATGC	2 cut(s)	901, 1180
MvaI	CCWGG	1 cut(s)	416
MvnI	CGCG	3 cut(s)	572, 1036, 1480
MwoI	GCNNNNNNNGC	9 cut(s)	30, 39, 304, 406, 409, 432, 435, 1153, 1328
NciI	CCSGG	3 cut(s)	255, 256, 804
NdeI	CATATG	1 cut(s)	871
NdeII	GATC	5 cut(s)	658, 748, 874, 997, 1281
NlaIII	CATG	7 cut(s)	311, 791, 828, 998, 1009, 1062, 1144
NlaIV	GGNNCC	5 cut(s)	70, 660, 816, 1023, 1476
NmuCI	GTSAC	7 cut(s)	511, 626, 806, 932, 1241, 1291, 1310
NotI	GCGGCCGC	1 cut(s)	424
NsiI	ATGCAT	1 cut(s)	1166
NspI	RCATGY	2 cut(s)	791, 1062
OliI	CACNNNNGTG	2 cut(s)	518, 939
PaeI	GCATGC	1 cut(s)	1062
PcsI	WCGNNNNNNNCGW	3 cut(s)	171, 204, 336
PctI	GAATGC	2 cut(s)	901, 1180
PfeI	GAWTC	1 cut(s)	122
PflFI	GACNNNGTC	1 cut(s)	194
PfoI	TCCNGGA	1 cut(s)	414
PleI	GAGTC	6 cut(s)	175, 244, 371, 1039, 1238, 1472
PpsI	GAGTC	6 cut(s)	175, 244, 371, 1039, 1238, 1472
PpuMI	RGGWCCY	1 cut(s)	815
Psp5II	RGGWCCY	1 cut(s)	815
Psp6I	CCWGG	1 cut(s)	414
PspEI	GGTNACC	1 cut(s)	932
PspFI	CCCAGC	1 cut(s)	1504
PspGI	CCWGG	1 cut(s)	414
PspN4I	GGNNCC	5 cut(s)	70, 660, 816, 1023, 1476
PspPI	GGNCC	3 cut(s)	621, 815, 1137
PspPPI	RGGWCCY	1 cut(s)	815
PstI	CTGCAG	1 cut(s)	520
PstNI	CAGNNNCTG	1 cut(s)	1331
PsuI	RGATCY	1 cut(s)	658
PsyI	GACNNNGTC	1 cut(s)	194
PvuII	CAGCTG	1 cut(s)	1508
RsaI	GTAC	1 cut(s)	778
RsaNI	GTAC	1 cut(s)	777
RseI	CAYNNNNRTG	5 cut(s)	518, 585, 939, 1069, 1203
SaqAI	TTAA	5 cut(s)	830, 885, 1170, 1419, 1539
Sau3AI	GATC	5 cut(s)	658, 748, 874, 997, 1281
Sau96I	GGNCC	3 cut(s)	621, 815, 1137
SchI	GAGTC	6 cut(s)	176, 244, 371, 1040, 1239, 1473
ScrFI	CCNGG	4 cut(s)	255, 256, 416, 804
SduI	GDGCHC	1 cut(s)	1054
SfaNI	GCATC	3 cut(s)	508, 566, 1331
SfcI	CTRYAG	1 cut(s)	516
SinI	GGWCC	2 cut(s)	815, 1137
SmaI	CCCGGG	1 cut(s)	256
SmiMI	CAYNNNNRTG	5 cut(s)	518, 585, 939, 1069, 1203
SmlI	CTYRAG	1 cut(s)	1538
SmoI	CTYRAG	1 cut(s)	1538
SphI	GCATGC	1 cut(s)	1062
Sse9I	AATT	2 cut(s)	1097, 1420
SspMI	CTAG	3 cut(s)	450, 665, 1454
StyD4I	CCNGG	4 cut(s)	253, 254, 414, 802
StyI	CCWWGG	3 cut(s)	51, 390, 937
TaaI	ACNGT	3 cut(s)	883, 988, 1397
TaqI	TCGA	3 cut(s)	877, 1000, 1303
TasI	AATT	2 cut(s)	1097, 1420
TauI	GCSGC	9 cut(s)	27, 30, 33, 136, 230, 426, 429, 438, 1322
TfiI	GAWTC	1 cut(s)	122
Tru1I	TTAA	5 cut(s)	830, 885, 1170, 1419, 1539
Tru9I	TTAA	5 cut(s)	830, 885, 1170, 1419, 1539
TscAI	CASTG	5 cut(s)	520, 525, 623, 631, 1315
TseFI	GTSAC	7 cut(s)	511, 626, 806, 932, 1241, 1291, 1310
TseI	GCWGC	7 cut(s)	400, 403, 406, 409, 1144, 1193, 1508
Tsp45I	GTSAC	7 cut(s)	511, 626, 806, 932, 1241, 1291, 1310
TspDTI	ATGAA	6 cut(s)	17, 195, 749, 858, 1110, 1254
TspGWI	ACGGA	2 cut(s)	982, 1037
TspMI	CCCGGG	1 cut(s)	254
TspRI	CASTG	5 cut(s)	520, 525, 623, 631, 1315
Tth111I	GACNNNGTC	1 cut(s)	194
Vha464I	CTTAAG	1 cut(s)	1538
VpaK11BI	GGWCC	2 cut(s)	815, 1137
XapI	RAATTY	1 cut(s)	1097
XceI	RCATGY	2 cut(s)	791, 1062
XmaI	CCCGGG	1 cut(s)	254
XspI	CTAG	3 cut(s)	450, 665, 1454
Zsp2I	ATGCAT	1 cut(s)	1166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.