Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

40899601 .. 40903536

Gene Structure 3936 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1274300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1563 bp

ATGAAGAACAAGAAGAGAGGCGGCGGCTCAGCTCCCAAGGGACCTAAAAAGGGCAAGGCTTTCTCTCTCGACGATGACCCCTTCTTCGGCACCGAATCCAAGAAGCGCCGGAAAGTCCAGCTCGATGAGATTGAGAGCGAATCCGACGCCGACGTTGGGTCGGACGGCGAGGGCGGCGGAGAGGAGGCCGAGGAGGAGGAGATGGAGGCTGTAGTGGAAACGGCAGACGAGAAGAGGAATAGGCTTGCGCATGAGTTTTTGGAGAACACTCGGGAACTGCTGAGGAGGGCGAAGGAAGATGAGGATGATGATAGTGGTGAAGAAGATGTGAAGGAAGGTGAGATGGACTCGCTTGTTGGCAAGATGTTGCAGCAGCAGCAGCTCGAGGACAGCGGCCGAGCTCGCCGTGCCATTGCTTCTAGGGTTAAAAAGCCAGAACCAACTGATGAATTTCGGATATTAGTGAAGCATCGACAACCTGTTACTGCTGTGGCTTTGTCTGAGGTTGACCTCAGGGGCTTTTCAGCTTCTAAGGATGGCGCCATTCTACATTGGGATGTAGATAGTGGGAAAAGTGAAAAGTATCTATGGCCTAGTGATGCAGTATTAAAGTCCCACGGGGTCAAGGATCCACAAGGTCGAGCTAAAAAGCATAGTAAACATGTTTTAGCACTAGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATGTTCATTTGTGGGATACTCGTACACGAGAGCATATTCTGGCTTTTCCAGGTCACAGGGGACCTGTTTCATGTTTAACTTTCAGGCAAGGGTCGTCAGAACTGTTTTCTGGTTCATATGATAGAACAGTTAAGATATGGAATGTAGAAGATAGAGCTTACATAAACACATTATTTGGTCATCAAGGTGAAGTTTTAAGTATTGATTGCCTACGGAAAGAAAGGGTGTTGACTGTTGGACATGATCGAAGTATGCAGTTATTTAAGGTCCCAGAGGAGTCTCGTTTAGTATTCCGTGCCCCTGCATCTTCTTTGGAATGTTGTTGTTTTATTAGCAATGACGAATTCTTATCCGGCTCTGATGATGGAAGTGTTGAGCTTTGGGACATGCTGCGGAAGAAGCCTGTTTGCATAGCTAAGAATGCTCATCCTCTGTTGGCTGCACAGAAAAATGCTGAACCAAAGGATGGTGATAGAATCCCAAATGGTCACATAGAAAATGGTAACCACACTTCTGAAAGCTACGATTGTTCATCAACAAATTCTTGGGTTAGTTCAGTCGCTGTTTGTAGCGGCAGTGACCTCGCTGCATCAGGAGCTGGTAACGGCTCTGTTCGATTATGGGCTATTAAAAGTGAAAGCAAAGTGATTCAGCCGTTACACAACATCCCATTGGTTGGATTTGTGAATTCGTTAGCTTTTGAAAAATCTGGACAGTTTCTAGTCGCAGGAGTTGGGAAGGAACCTCGTCTAGGAAGGTGGGGACATAACCCAGCTGCTCGGAATGGAATCGCAATTTATTCCCTTAAGCTTTCATCAGAAGATACAAATACTCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

521

Amino Acids

57.21

Weight (kDa)

5.99

Isoelectric Point (pI)

45.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	151 - 331	1.6e-17	CDC20/Fizzy WD40 domain
WDR55	PF24796	152 - 376	1.8e-14	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	156 - 328	2.1e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	175 - 329	3.3e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	196 - 328	2.5e-15	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	209 - 328	1.1e-07	WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	214 - 331	6.8e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	215 - 376	4.2e-25	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	215 - 302	2.3e-16	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	216 - 331	2.2e-24	WDR3 first beta-propeller domain
Beta-prop_SPT8	PF23798	216 - 381	7.1e-06	SPT8 beta propeller
WD40_WDHD1_1st	PF24817	216 - 276	2.9e-06	WDHD1 first WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	216 - 333	6.5e-06	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	217 - 247	2.8e-07	WD domain, G-beta repeat
Beta-prop_EML	PF23409	217 - 384	3.2e-10	Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta	PF25391	217 - 372	6.2e-15	G protein beta WD-40 repeat protein
EIF3I	PF24805	218 - 297	8.5e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	218 - 334	3.7e-14	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	219 - 377	3.4e-19	Prp19 WD40 domain
WD40	PF00400	254 - 289	5.8e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	260 - 375	1.8e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	249
AccB1I	GGYRCC	2 cut(s)	89, 539
AccB7I	CCANNNNNTGG	2 cut(s)	1190, 1400
AciI	CCGC	7 cut(s)	21, 24, 174, 177, 393, 1117, 1296
AclWI	GGATC	2 cut(s)	623, 636
AcoI	YGGCCR	1 cut(s)	394
AcsI	RAATTY	4 cut(s)	449, 1067, 1264, 1411
AcyI	GRCGYC	2 cut(s)	147, 540
AfaI	GTAC	1 cut(s)	748
AfiI	CCNNNNNNNGG	6 cut(s)	50, 86, 156, 1190, 1400, 1475
AflII	CTTAAG	1 cut(s)	1529
AflIII	ACRYGT	1 cut(s)	661
AgsI	TTSAA	1 cut(s)	1427
AhdI	GACNNNNNGTC	1 cut(s)	157
AjnI	CCWGG	1 cut(s)	772
Alw21I	GWGCWC	1 cut(s)	403
Alw26I	GTCTC	1 cut(s)	1008
AlwI	GGATC	2 cut(s)	623, 636
AlwNI	CAGNNNCTG	2 cut(s)	1286, 1322
Ama87I	CYCGRG	2 cut(s)	270, 383
AoxI	GGCC	3 cut(s)	186, 394, 590
ApeKI	GCWGC	8 cut(s)	370, 373, 376, 379, 1114, 1163, 1310, 1499
ApoI	RAATTY	4 cut(s)	449, 1067, 1264, 1411
ArsI	GACNNNNNNTTYG	2 cut(s)	68, 100
AspLEI	GCGC	3 cut(s)	108, 250, 542
AspS9I	GGNCC	3 cut(s)	41, 785, 991
AsuHPI	GGTGA	4 cut(s)	329, 350, 923, 1205
AvaI	CYCGRG	2 cut(s)	270, 383
AvaII	GGWCC	3 cut(s)	41, 785, 991
AxyI	CCTNAGG	1 cut(s)	512
BaeGI	GKGCMC	1 cut(s)	1024
BamHI	GGATCC	1 cut(s)	628
BanI	GGYRCC	2 cut(s)	89, 539
BanII	GRGCYC	1 cut(s)	403
BarI	GAAGNNNNNNTAC	2 cut(s)	1219, 1251
BauI	CACGAG	1 cut(s)	750
Bbv12I	GWGCWC	1 cut(s)	403
BbvCI	CCTCAGC	1 cut(s)	281
BbvI	GCAGC	8 cut(s)	382, 385, 388, 391, 1101, 1150, 1297, 1486
BccI	CCATC	6 cut(s)	196, 337, 530, 683, 1082, 1184
BceAI	ACGGC	5 cut(s)	181, 237, 390, 1345, 1363
BcgI	CGANNNNNNTGC	2 cut(s)	1288, 1322
BciT130I	CCWGG	1 cut(s)	774
BciVI	GTATCC	1 cut(s)	733
BcoDI	GTCTC	1 cut(s)	1008
BfaI	CTAG	5 cut(s)	420, 594, 674, 1445, 1475
BfmI	CTRYAG	1 cut(s)	210
BfoI	RGCGCY	2 cut(s)	109, 543
BfrI	CTTAAG	1 cut(s)	1529
BfuI	GTATCC	1 cut(s)	733
BlpI	GCTNAGC	1 cut(s)	28
Bme1390I	CCNGG	1 cut(s)	774
Bme18I	GGWCC	3 cut(s)	41, 785, 991
BmeRI	GACNNNNNGTC	1 cut(s)	157
BmeT110I	CYCGRG	2 cut(s)	270, 383
BmgT120I	GGNCC	3 cut(s)	41, 785, 991
BmiI	GGNNCC	7 cut(s)	42, 91, 541, 630, 786, 993, 1467
BmrFI	CCNGG	1 cut(s)	774
BmsI	GCATC	4 cut(s)	478, 589, 1037, 1322
BplI	GAGNNNNNCTC	2 cut(s)	332, 364
Bpu10I	CCTNAGC	1 cut(s)	281
Bpu1102I	GCTNAGC	1 cut(s)	28
BsaHI	GRCGYC	2 cut(s)	147, 540
BsaJI	CCNNGG	3 cut(s)	36, 189, 616
BsaXI	ACNNNNNCTCC	1 cut(s)	1540
Bsc4I	CCNNNNNNNGG	6 cut(s)	50, 86, 156, 1190, 1400, 1475
Bse21I	CCTNAGG	1 cut(s)	512
Bse3DI	GCAATG	2 cut(s)	411, 1066
BseBI	CCWGG	1 cut(s)	774
BseDI	CCNNGG	3 cut(s)	36, 189, 616
BseGI	GGATG	6 cut(s)	310, 541, 562, 1150, 1195, 1389
BseLI	CCNNNNNNNGG	6 cut(s)	50, 86, 156, 1190, 1400, 1475
BseMI	GCAATG	2 cut(s)	411, 1066
BseMII	CTCAG	4 cut(s)	42, 272, 492, 526
BseRI	GAGGAG	6 cut(s)	197, 206, 209, 212, 298, 1013
BseSI	GKGCMC	1 cut(s)	1024
BseX3I	CGGCCG	1 cut(s)	394
BseXI	GCAGC	8 cut(s)	382, 385, 388, 391, 1101, 1150, 1297, 1486
BseYI	CCCAGC	1 cut(s)	1495
BsgI	GTGCAG	1 cut(s)	1149
Bsh1285I	CGRYCG	1 cut(s)	397
BshFI	GGCC	3 cut(s)	188, 396, 592
BshNI	GGYRCC	2 cut(s)	89, 539
BsiEI	CGRYCG	1 cut(s)	397
BsiHKAI	GWGCWC	1 cut(s)	403
BsiHKCI	CYCGRG	2 cut(s)	270, 383
BsiSI	CCGG	2 cut(s)	109, 1077
BslFI	GGGAC	6 cut(s)	54, 598, 798, 977, 1121, 1500
BslI	CCNNNNNNNGG	6 cut(s)	50, 86, 156, 1190, 1400, 1475
BsmAI	GTCTC	1 cut(s)	1008
BsmFI	GGGAC	6 cut(s)	54, 598, 798, 977, 1121, 1500
BsmI	GAATGC	1 cut(s)	1150
BsnI	GGCC	3 cut(s)	188, 396, 592
BsoBI	CYCGRG	2 cut(s)	270, 383
Bsp1286I	GDGCHC	2 cut(s)	403, 1024
Bsp143I	GATC	3 cut(s)	628, 718, 967
Bsp1720I	GCTNAGC	1 cut(s)	28
BspACI	CCGC	7 cut(s)	21, 24, 174, 177, 393, 1117, 1296
BspANI	GGCC	3 cut(s)	188, 396, 592
BspCNI	CTCAG	4 cut(s)	41, 273, 493, 525
BspLI	GGNNCC	7 cut(s)	42, 91, 541, 630, 786, 993, 1467
BspPI	GGATC	2 cut(s)	623, 636
BspT107I	GGYRCC	2 cut(s)	89, 539
BspTI	CTTAAG	1 cut(s)	1529
BsrDI	GCAATG	2 cut(s)	411, 1066
BssECI	CCNNGG	3 cut(s)	36, 189, 616
BssMI	GATC	3 cut(s)	628, 718, 967
BssNI	GRCGYC	2 cut(s)	147, 540
BssSI	CACGAG	1 cut(s)	750
BssT1I	CCWWGG	1 cut(s)	36
Bst2BI	CACGAG	1 cut(s)	750
Bst2UI	CCWGG	1 cut(s)	774
Bst4CI	ACNGT	4 cut(s)	828, 853, 958, 1440
Bst6I	CTCTTC	2 cut(s)	8, 227
BstACI	GRCGYC	2 cut(s)	147, 540
BstAFI	CTTAAG	1 cut(s)	1529
BstC8I	GCNNGC	2 cut(s)	246, 403
BstDEI	CTNAG	6 cut(s)	28, 281, 501, 512, 531, 1140
BstDSI	CCRYGG	1 cut(s)	616
BstEII	GGTNACC	1 cut(s)	1226
BstENI	CCTNNNNNAGG	1 cut(s)	1473
BstF5I	GGATG	6 cut(s)	310, 541, 562, 1150, 1195, 1389
BstH2I	RGCGCY	2 cut(s)	109, 543
BstHHI	GCGC	3 cut(s)	108, 250, 542
BstKTI	GATC	3 cut(s)	631, 721, 970
BstMAI	GTCTC	1 cut(s)	1008
BstMBI	GATC	3 cut(s)	628, 718, 967
BstMCI	CGRYCG	1 cut(s)	397
BstMWI	GCNNNNNNNGC	8 cut(s)	174, 376, 379, 402, 407, 1123, 1145, 1319
BstNI	CCWGG	1 cut(s)	774
BstNSI	RCATGY	2 cut(s)	665, 1114
BstPI	GGTNACC	1 cut(s)	1226
BstSCI	CCNGG	1 cut(s)	772
BstSFI	CTRYAG	1 cut(s)	210
BstSLI	GKGCMC	1 cut(s)	1024
BstV1I	GCAGC	8 cut(s)	382, 385, 388, 391, 1101, 1150, 1297, 1486
BstX2I	RGATCY	1 cut(s)	628
BstYI	RGATCY	1 cut(s)	628
BstZI	CGGCCG	1 cut(s)	394
Bsu36I	CCTNAGG	1 cut(s)	512
BsuI	GTATCC	1 cut(s)	733
BsuRI	GGCC	3 cut(s)	188, 396, 592
BtgI	CCRYGG	1 cut(s)	616
BtsCI	GGATG	6 cut(s)	310, 541, 562, 1150, 1195, 1389
BtsI	GCAGTG	1 cut(s)	1306
BtsIMutI	CAGTG	1 cut(s)	1306
Cac8I	GCNNGC	2 cut(s)	246, 403
CaiI	CAGNNNCTG	2 cut(s)	1286, 1322
CfoI	GCGC	3 cut(s)	108, 250, 542
Cfr13I	GGNCC	3 cut(s)	41, 785, 991
CseI	GACGC	1 cut(s)	155
Csp6I	GTAC	1 cut(s)	747
CviAII	CATG	6 cut(s)	251, 662, 725, 795, 965, 1111
CviQI	GTAC	1 cut(s)	747
DdeI	CTNAG	6 cut(s)	28, 281, 501, 512, 531, 1140
DinI	GGCGCC	1 cut(s)	541
DpnI	GATC	3 cut(s)	630, 720, 969
DpnII	GATC	3 cut(s)	628, 718, 967
DriI	GACNNNNNGTC	1 cut(s)	157
EaeI	YGGCCR	1 cut(s)	394
EagI	CGGCCG	1 cut(s)	394
Eam1104I	CTCTTC	2 cut(s)	8, 227
Eam1105I	GACNNNNNGTC	1 cut(s)	157
EarI	CTCTTC	2 cut(s)	8, 227
EciI	GGCGGA	1 cut(s)	192
Ecl136II	GAGCTC	1 cut(s)	401
EclXI	CGGCCG	1 cut(s)	394
Eco130I	CCWWGG	1 cut(s)	36
Eco24I	GRGCYC	1 cut(s)	403
Eco47I	GGWCC	3 cut(s)	41, 785, 991
Eco52I	CGGCCG	1 cut(s)	394
Eco53kI	GAGCTC	1 cut(s)	401
Eco81I	CCTNAGG	1 cut(s)	512
Eco88I	CYCGRG	2 cut(s)	270, 383
Eco91I	GGTNACC	1 cut(s)	1226
EcoICRI	GAGCTC	1 cut(s)	401
EcoNI	CCTNNNNNAGG	1 cut(s)	1473
EcoO109I	RGGNCCY	3 cut(s)	41, 785, 991
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRI	GAATTC	2 cut(s)	1067, 1411
EcoRII	CCWGG	1 cut(s)	772
EcoT14I	CCWWGG	1 cut(s)	36
EcoT38I	GRGCYC	1 cut(s)	403
EgeI	GGCGCC	1 cut(s)	541
EheI	GGCGCC	1 cut(s)	541
ErhI	CCWWGG	1 cut(s)	36
FaeI	CATG	6 cut(s)	254, 665, 728, 798, 968, 1114
FaqI	GGGAC	6 cut(s)	54, 598, 798, 977, 1121, 1500
FatI	CATG	6 cut(s)	250, 661, 724, 794, 964, 1110
FauNDI	CATATG	1 cut(s)	841
FokI	GGATG	6 cut(s)	317, 548, 569, 1137, 1202, 1376
FriOI	GRGCYC	1 cut(s)	403
FspBI	CTAG	5 cut(s)	420, 594, 674, 1445, 1475
FspI	TGCGCA	1 cut(s)	249
GlaI	GCGC	3 cut(s)	107, 249, 541
GsaI	CCCAGC	1 cut(s)	1499
HaeII	RGCGCY	2 cut(s)	109, 543
HaeIII	GGCC	3 cut(s)	188, 396, 592
HapII	CCGG	2 cut(s)	109, 1077
HgaI	GACGC	1 cut(s)	155
HhaI	GCGC	3 cut(s)	108, 250, 542
Hin1I	GRCGYC	2 cut(s)	147, 540
Hin1II	CATG	6 cut(s)	254, 665, 728, 798, 968, 1114
Hin6I	GCGC	3 cut(s)	106, 248, 540
HinP1I	GCGC	3 cut(s)	106, 248, 540
HincII	GTYRAC	2 cut(s)	508, 954
HindII	GTYRAC	2 cut(s)	508, 954
HindIII	AAGCTT	1 cut(s)	1532
HinfI	GANTC	7 cut(s)	95, 140, 347, 1001, 1200, 1372, 1512
HpaII	CCGG	2 cut(s)	109, 1077
HphI	GGTGA	4 cut(s)	329, 350, 923, 1205
Hpy166II	GTNNAC	4 cut(s)	508, 659, 749, 954
Hpy188III	TCNNGA	4 cut(s)	68, 272, 1317, 1434
Hpy8I	GTNNAC	4 cut(s)	508, 659, 749, 954
Hpy99I	CGWCG	3 cut(s)	74, 149, 155
HpyAV	CCTTC	6 cut(s)	91, 286, 325, 329, 1456, 1473
HpyCH4III	ACNGT	4 cut(s)	828, 853, 958, 1440
HpyCH4IV	ACGT	1 cut(s)	153
HpyCH4V	TGCA	7 cut(s)	370, 602, 979, 1028, 1134, 1166, 1313
HpyF10VI	GCNNNNNNNGC	8 cut(s)	174, 376, 379, 402, 407, 1123, 1145, 1319
HpyF3I	CTNAG	6 cut(s)	28, 281, 501, 512, 531, 1140
HpySE526I	ACGT	1 cut(s)	153
Hsp92I	GRCGYC	2 cut(s)	147, 540
Hsp92II	CATG	6 cut(s)	254, 665, 728, 798, 968, 1114
HspAI	GCGC	3 cut(s)	106, 248, 540
KasI	GGCGCC	1 cut(s)	539
Kzo9I	GATC	3 cut(s)	628, 718, 967
LmnI	GCTCC	2 cut(s)	37, 1319
Lsp1109I	GCAGC	8 cut(s)	382, 385, 388, 391, 1101, 1150, 1297, 1486
LweI	GCATC	4 cut(s)	478, 589, 1037, 1322
MaeI	CTAG	5 cut(s)	420, 594, 674, 1445, 1475
MaeII	ACGT	1 cut(s)	153
MaeIII	GTNAC	7 cut(s)	481, 776, 1211, 1226, 1301, 1325, 1380
MalI	GATC	3 cut(s)	630, 720, 969
MboI	GATC	3 cut(s)	628, 718, 967
MflI	RGATCY	1 cut(s)	628
MhlI	GDGCHC	2 cut(s)	403, 1024
MluCI	AATT	5 cut(s)	449, 1067, 1264, 1411, 1518
Mly113I	GGCGCC	1 cut(s)	540
MlyI	GAGTC	2 cut(s)	341, 1010
MmeI	TCCRAC	4 cut(s)	141, 168, 940, 1381
MseI	TTAA	8 cut(s)	426, 608, 800, 855, 920, 987, 1353, 1530
MslI	CAYNNNNRTG	2 cut(s)	555, 909
MspA1I	CMGCKG	2 cut(s)	393, 1499
MspCI	CTTAAG	1 cut(s)	1529
MspI	CCGG	2 cut(s)	109, 1077
MspR9I	CCNGG	1 cut(s)	774
Mva1269I	GAATGC	1 cut(s)	1150
MvaI	CCWGG	1 cut(s)	774
MwoI	GCNNNNNNNGC	8 cut(s)	174, 376, 379, 402, 407, 1123, 1145, 1319
NarI	GGCGCC	1 cut(s)	540
NdeI	CATATG	1 cut(s)	841
NdeII	GATC	3 cut(s)	628, 718, 967
NlaIII	CATG	6 cut(s)	254, 665, 728, 798, 968, 1114
NlaIV	GGNNCC	7 cut(s)	42, 91, 541, 630, 786, 993, 1467
NmeAIII	GCCGAG	2 cut(s)	214, 422
NmuCI	GTSAC	3 cut(s)	776, 1211, 1301
NsbI	TGCGCA	1 cut(s)	249
NspI	RCATGY	2 cut(s)	665, 1114
PaeR7I	CTCGAG	1 cut(s)	383
PciI	ACATGT	1 cut(s)	661
PctI	GAATGC	1 cut(s)	1150
PfeI	GAWTC	5 cut(s)	95, 140, 1200, 1372, 1512
PflMI	CCANNNNNTGG	2 cut(s)	1190, 1400
PleI	GAGTC	2 cut(s)	341, 1009
PluTI	GGCGCC	1 cut(s)	543
PpsI	GAGTC	2 cut(s)	341, 1009
PpuMI	RGGWCCY	3 cut(s)	41, 785, 991
PscI	ACATGT	1 cut(s)	661
Psp124BI	GAGCTC	1 cut(s)	403
Psp5II	RGGWCCY	3 cut(s)	41, 785, 991
Psp6I	CCWGG	1 cut(s)	772
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	1 cut(s)	1495
PspGI	CCWGG	1 cut(s)	772
PspN4I	GGNNCC	7 cut(s)	42, 91, 541, 630, 786, 993, 1467
PspPI	GGNCC	3 cut(s)	41, 785, 991
PspPPI	RGGWCCY	3 cut(s)	41, 785, 991
PspXI	VCTCGAGB	1 cut(s)	383
PstNI	CAGNNNCTG	2 cut(s)	1286, 1322
PsuI	RGATCY	1 cut(s)	628
PvuII	CAGCTG	1 cut(s)	1499
RsaI	GTAC	1 cut(s)	748
RsaNI	GTAC	1 cut(s)	747
RseI	CAYNNNNRTG	2 cut(s)	555, 909
SacI	GAGCTC	1 cut(s)	403
SaqAI	TTAA	8 cut(s)	426, 608, 800, 855, 920, 987, 1353, 1530
Sau3AI	GATC	3 cut(s)	628, 718, 967
Sau96I	GGNCC	3 cut(s)	41, 785, 991
SchI	GAGTC	2 cut(s)	341, 1010
ScrFI	CCNGG	1 cut(s)	774
SduI	GDGCHC	2 cut(s)	403, 1024
SfaNI	GCATC	4 cut(s)	478, 589, 1037, 1322
SfcI	CTRYAG	1 cut(s)	210
SfoI	GGCGCC	1 cut(s)	541
Sfr274I	CTCGAG	1 cut(s)	383
SinI	GGWCC	3 cut(s)	41, 785, 991
SlaI	CTCGAG	1 cut(s)	383
SmiMI	CAYNNNNRTG	2 cut(s)	555, 909
SmlI	CTYRAG	2 cut(s)	383, 1529
SmoI	CTYRAG	2 cut(s)	383, 1529
Sse9I	AATT	5 cut(s)	449, 1067, 1264, 1411, 1518
SsiI	CCGC	7 cut(s)	21, 24, 174, 177, 393, 1117, 1296
SspDI	GGCGCC	1 cut(s)	539
SspMI	CTAG	5 cut(s)	420, 594, 674, 1445, 1475
SstI	GAGCTC	1 cut(s)	403
StyD4I	CCNGG	1 cut(s)	772
StyI	CCWWGG	1 cut(s)	36
TaaI	ACNGT	4 cut(s)	828, 853, 958, 1440
TaiI	ACGT	1 cut(s)	156
TaqI	TCGA	7 cut(s)	69, 123, 384, 472, 640, 970, 1339
TasI	AATT	5 cut(s)	449, 1067, 1264, 1411, 1518
TauI	GCSGC	5 cut(s)	24, 27, 177, 396, 1299
TfiI	GAWTC	5 cut(s)	95, 140, 1200, 1372, 1512
Tru1I	TTAA	8 cut(s)	426, 608, 800, 855, 920, 987, 1353, 1530
Tru9I	TTAA	8 cut(s)	426, 608, 800, 855, 920, 987, 1353, 1530
TscAI	CASTG	1 cut(s)	1306
TseFI	GTSAC	3 cut(s)	776, 1211, 1301
TseI	GCWGC	8 cut(s)	370, 373, 376, 379, 1114, 1163, 1310, 1499
Tsp45I	GTSAC	3 cut(s)	776, 1211, 1301
TspDTI	ATGAA	7 cut(s)	17, 462, 719, 783, 828, 1245, 1527
TspGWI	ACGGA	2 cut(s)	952, 1007
TspRI	CASTG	1 cut(s)	1306
Van91I	CCANNNNNTGG	2 cut(s)	1190, 1400
Vha464I	CTTAAG	1 cut(s)	1529
VpaK11BI	GGWCC	3 cut(s)	41, 785, 991
XagI	CCTNNNNNAGG	1 cut(s)	1473
XapI	RAATTY	4 cut(s)	449, 1067, 1264, 1411
XceI	RCATGY	2 cut(s)	665, 1114
XhoI	CTCGAG	1 cut(s)	383
XspI	CTAG	5 cut(s)	420, 594, 674, 1445, 1475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.