Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Reverse (-)

82979919 .. 82983762

Gene Structure 3844 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00060730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGAACAGGAACAAGAAGCGAGGCGGCGGCGGCGGCGGCGGAGGCCGAGGCTCAGCTCCCAAAGGCGGAGGAGGAGACAGAAAGGGAGCTAAAAAAGGCAAGAGGCAGTCCTTCAAGGAAGACCCATTTTTCACCAACGAATCCAAGAAGCGGCTCAAATACGACGAGGACATTCGGAGCGACGAGTCGTCCGACGACGACCGAAACGACGCCGTCGGTAGTGGCGAGGAGGAGGAGGAGGCGGCTGAGGAGATTATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTTGGAGAGCATGAGAGGAAGAAGAAGGAAGACGAGGATGACGATAGCGATGACGACAGCGAGAAGGAAGGCGACAGAGACTCGCTTGTGGCCAAGGTTTTGCAGCAGCAGCAGCTCCAGGACAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCAGAAGCTACTGATGAGTTTCGGATCTTAGTGAAGCATCGCCAATCTGTCACCGCAGTGGTTCTCTCTGACGATGATGTCAGAGGTTTTTCAGCATCTAAGGACGGCACGATTTTGCATTGGGATGTAGATAGTGGGAAAAAAGAGAAGTATCAGTGGCCCAGTGACGAAGTATTGAAGTCCCACGGGGTCAAGGATCCACTAGGTAGAGCTACAAAGCATAGCAAACAAATGTTGGCACTTGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATATTCATTTGTGGGATACTCGTACCAGACAGCATGTTCAGGCTTTTCCGGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCAGAATTCTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGCAGAAGATAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCGGAGGAGTCGCGTTTAGTATTCCGTGCCCCTGCATGCTCATTGGAGTGTTGTTCTTTTATTAGCAATGATGAATTCTTATCCGGCTCTGATAATGGAAGTGTTGAGCTTTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTTTGTTAGCTGCACACAAAGATGTTGAACAGAAGGATGGTGATAGAGTCCCCAATGGTCACATAGAAAGCTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGTAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATTCAGCCCTTACACAGTCTCCCTGTGGTTGGATTTGTTAATTCTTTAGCTATTGCGAAAGCTGGAGGCTTTCTAGTTGCAGGAGTCGGAAAGGAACCGCGTCTAGGAAGGTGGGGACTTATCCCAGCTGCTCAGAATGGAGTTGCAATCCACACCCTTAAGCTTTCGTCAGATACACATAAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

527

Amino Acids

58.31

Weight (kDa)

7.0

Isoelectric Point (pI)

50.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	162 - 347	9.5e-17	CDC20/Fizzy WD40 domain
WDR55	PF24796	164 - 326	1.6e-07	WDR55
Beta-prop_DCAF12	PF23760	172 - 344	6.8e-06	DDB1- and CUL4-associated factor 12 beta propeller
Beta-prop_WDR36-Utp21_1st	PF25171	192 - 342	2.6e-06	WDR36/Utp21 first beta-propeller
NBCH_WD40	PF20426	204 - 328	6.6e-06	Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd	PF25047	212 - 341	8.1e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	227 - 285	2.3e-10	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	229 - 345	1.7e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	231 - 391	1.8e-22	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	231 - 399	1.1e-10	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	231 - 262	3.3e-06	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	232 - 346	1.5e-22	WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	232 - 349	1.7e-08	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	234 - 386	1.7e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	234 - 338	7.8e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	235 - 394	6e-19	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	235 - 349	1.4e-15	WDR3 second beta-propeller domain
WD40	PF00400	268 - 304	4.8e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	554
AccBSI	CCGCTC	1 cut(s)	453
AccII	CGCG	2 cut(s)	1051, 1495
AccIII	TCCGGA	1 cut(s)	1039
AclWI	GGATC	4 cut(s)	509, 668, 681, 1304
AcoI	YGGCCR	2 cut(s)	402, 439
AcsI	RAATTY	2 cut(s)	869, 1112
AcuI	CTGAAG	1 cut(s)	326
AcyI	GRCGYC	1 cut(s)	210
AfaI	GTAC	1 cut(s)	793
AfiI	CCNNNNNNNGG	4 cut(s)	65, 150, 1424, 1499
AflII	CTTAAG	1 cut(s)	1553
AgsI	TTSAA	5 cut(s)	115, 655, 1033, 1226, 1379
AhdI	GACNNNNNGTC	1 cut(s)	187
AjnI	CCWGG	1 cut(s)	429
AleI	CACNNNNGTG	2 cut(s)	533, 954
Alw26I	GTCTC	4 cut(s)	69, 257, 384, 1418
AlwI	GGATC	4 cut(s)	509, 668, 681, 1304
AlwNI	CAGNNNCTG	1 cut(s)	1346
Ama87I	CYCGRG	1 cut(s)	269
Aor13HI	TCCGGA	1 cut(s)	1039
AoxI	GGCC	4 cut(s)	43, 402, 439, 635
ApeKI	GCWGC	7 cut(s)	415, 418, 421, 424, 1159, 1208, 1523
ApoI	RAATTY	2 cut(s)	869, 1112
AspS9I	GGNCC	3 cut(s)	636, 830, 1152
AsuC2I	CCSGG	3 cut(s)	270, 271, 819
AsuHPI	GGTGA	5 cut(s)	124, 520, 941, 968, 1250
AvaI	CYCGRG	1 cut(s)	269
AvaII	GGWCC	2 cut(s)	830, 1152
BaeGI	GKGCMC	1 cut(s)	1069
BalI	TGGCCA	1 cut(s)	404
BamHI	GGATCC	1 cut(s)	673
BbsI	GAAGAC	2 cut(s)	126, 348
BbvCI	CCTCAGC	2 cut(s)	246, 284
BbvI	GCAGC	7 cut(s)	427, 430, 433, 436, 1146, 1195, 1510
BccI	CCATC	2 cut(s)	728, 1229
BceAI	ACGGC	3 cut(s)	197, 598, 1007
BciT130I	CCWGG	1 cut(s)	431
BciVI	GTATCC	1 cut(s)	778
BcnI	CCSGG	3 cut(s)	270, 271, 819
BcoDI	GTCTC	4 cut(s)	69, 257, 384, 1418
BfaI	CTAG	4 cut(s)	465, 680, 1469, 1499
BfrI	CTTAAG	1 cut(s)	1553
BfuI	GTATCC	1 cut(s)	778
BlpI	GCTNAGC	1 cut(s)	52
Bme1390I	CCNGG	4 cut(s)	270, 271, 431, 819
Bme18I	GGWCC	2 cut(s)	830, 1152
BmeRI	GACNNNNNGTC	1 cut(s)	187
BmeT110I	CYCGRG	1 cut(s)	269
BmgT120I	GGNCC	3 cut(s)	636, 830, 1152
BmiI	GGNNCC	4 cut(s)	675, 831, 1038, 1491
BmrFI	CCNGG	4 cut(s)	270, 271, 431, 819
BmrI	ACTGGG	1 cut(s)	633
BmsI	GCATC	3 cut(s)	523, 581, 1346
BmuI	ACTGGG	1 cut(s)	633
BpiI	GAAGAC	2 cut(s)	126, 348
BpmI	CTGGAG	2 cut(s)	413, 1479
Bpu10I	CCTNAGC	2 cut(s)	246, 284
Bpu1102I	GCTNAGC	1 cut(s)	52
BpuMI	CCSGG	3 cut(s)	270, 271, 819
BsaHI	GRCGYC	1 cut(s)	210
BsaJI	CCNNGG	6 cut(s)	46, 269, 270, 405, 661, 952
BsaWI	WCCGGW	1 cut(s)	1039
BsaXI	ACNNNNNCTCC	2 cut(s)	1398, 1428
Bsc4I	CCNNNNNNNGG	4 cut(s)	65, 150, 1424, 1499
Bse1I	ACTGG	1 cut(s)	639
Bse3DI	GCAATG	3 cut(s)	456, 1111, 1396
BseAI	TCCGGA	1 cut(s)	1039
BseBI	CCWGG	1 cut(s)	431
BseDI	CCNNGG	6 cut(s)	46, 269, 270, 405, 661, 952
BseGI	GGATG	4 cut(s)	355, 607, 1195, 1240
BseLI	CCNNNNNNNGG	4 cut(s)	65, 150, 1424, 1499
BseMI	GCAATG	3 cut(s)	456, 1111, 1396
BseMII	CTCAG	4 cut(s)	66, 237, 275, 1541
BseNI	ACTGG	1 cut(s)	639
BseRI	GAGGAG	9 cut(s)	84, 87, 242, 245, 248, 251, 263, 275, 1058
BseSI	GKGCMC	1 cut(s)	1069
BseX3I	CGGCCG	1 cut(s)	439
BseXI	GCAGC	7 cut(s)	427, 430, 433, 436, 1146, 1195, 1510
BseYI	CCCAGC	1 cut(s)	1519
BsgI	GTGCAG	1 cut(s)	1194
Bsh1236I	CGCG	2 cut(s)	1051, 1495
Bsh1285I	CGRYCG	2 cut(s)	202, 442
BshFI	GGCC	4 cut(s)	45, 404, 441, 637
BsiEI	CGRYCG	2 cut(s)	202, 442
BsiHKCI	CYCGRG	1 cut(s)	269
BsiSI	CCGG	4 cut(s)	270, 818, 1040, 1122
BslFI	GGGAC	4 cut(s)	643, 843, 1232, 1524
BslI	CCNNNNNNNGG	4 cut(s)	65, 150, 1424, 1499
BsmAI	GTCTC	4 cut(s)	69, 257, 384, 1418
BsmFI	GGGAC	4 cut(s)	643, 843, 1232, 1524
BsmI	GAATGC	2 cut(s)	916, 1195
BsnI	GGCC	4 cut(s)	45, 404, 441, 637
BsoBI	CYCGRG	1 cut(s)	269
Bsp1286I	GDGCHC	1 cut(s)	1069
Bsp13I	TCCGGA	1 cut(s)	1039
Bsp143I	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
Bsp1720I	GCTNAGC	1 cut(s)	52
BspANI	GGCC	4 cut(s)	45, 404, 441, 637
BspCNI	CTCAG	4 cut(s)	65, 238, 276, 1540
BspEI	TCCGGA	1 cut(s)	1039
BspFNI	CGCG	2 cut(s)	1051, 1495
BspLI	GGNNCC	4 cut(s)	675, 831, 1038, 1491
BspPI	GGATC	4 cut(s)	509, 668, 681, 1304
BspTI	CTTAAG	1 cut(s)	1553
BsrBI	CCGCTC	1 cut(s)	453
BsrDI	GCAATG	3 cut(s)	456, 1111, 1396
BsrI	ACTGG	1 cut(s)	639
BssECI	CCNNGG	6 cut(s)	46, 269, 270, 405, 661, 952
BssMI	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
BssNI	GRCGYC	1 cut(s)	210
BssT1I	CCWWGG	2 cut(s)	405, 952
Bst2UI	CCWGG	1 cut(s)	431
Bst4CI	ACNGT	3 cut(s)	898, 1003, 1412
Bst6I	CTCTTC	1 cut(s)	302
BstACI	GRCGYC	1 cut(s)	210
BstAFI	CTTAAG	1 cut(s)	1553
BstC8I	GCNNGC	1 cut(s)	1075
BstDEI	CTNAG	6 cut(s)	52, 246, 284, 505, 576, 1527
BstDSI	CCRYGG	1 cut(s)	661
BstEII	GGTNACC	1 cut(s)	947
BstF5I	GGATG	4 cut(s)	355, 607, 1195, 1240
BstFNI	CGCG	2 cut(s)	1051, 1495
BstKTI	GATC	6 cut(s)	504, 676, 766, 892, 1015, 1299
BstMAI	GTCTC	4 cut(s)	69, 257, 384, 1418
BstMBI	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
BstMCI	CGRYCG	2 cut(s)	202, 442
BstNI	CCWGG	1 cut(s)	431
BstNSI	RCATGY	2 cut(s)	806, 1077
BstPI	GGTNACC	1 cut(s)	947
BstSCI	CCNGG	4 cut(s)	268, 269, 429, 817
BstSLI	GKGCMC	1 cut(s)	1069
BstUI	CGCG	2 cut(s)	1051, 1495
BstV1I	GCAGC	7 cut(s)	427, 430, 433, 436, 1146, 1195, 1510
BstV2I	GAAGAC	2 cut(s)	126, 348
BstX2I	RGATCY	2 cut(s)	501, 673
BstYI	RGATCY	2 cut(s)	501, 673
BstZI	CGGCCG	1 cut(s)	439
BsuI	GTATCC	1 cut(s)	778
BsuRI	GGCC	4 cut(s)	45, 404, 441, 637
BtgI	CCRYGG	1 cut(s)	661
BtgZI	GCGATG	2 cut(s)	375, 500
BtsCI	GGATG	4 cut(s)	355, 607, 1195, 1240
BtsI	GCAGTG	2 cut(s)	540, 1330
BtsIMutI	CAGTG	4 cut(s)	540, 638, 646, 1330
Cac8I	GCNNGC	1 cut(s)	1075
CaiI	CAGNNNCTG	1 cut(s)	1346
CciNI	GCGGCCGC	1 cut(s)	439
Cfr13I	GGNCC	3 cut(s)	636, 830, 1152
Cfr9I	CCCGGG	1 cut(s)	269
CseI	GACGC	2 cut(s)	218, 1484
Csp6I	GTAC	1 cut(s)	792
CviAII	CATG	7 cut(s)	323, 803, 840, 1010, 1021, 1074, 1156
CviQI	GTAC	1 cut(s)	792
DdeI	CTNAG	6 cut(s)	52, 246, 284, 505, 576, 1527
DpnI	GATC	6 cut(s)	503, 675, 765, 891, 1014, 1298
DpnII	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
DrdI	GACNNNNNNGTC	1 cut(s)	554
DriI	GACNNNNNGTC	1 cut(s)	187
DseDI	GACNNNNNNGTC	1 cut(s)	554
EaeI	YGGCCR	2 cut(s)	402, 439
EagI	CGGCCG	1 cut(s)	439
Eam1104I	CTCTTC	1 cut(s)	302
Eam1105I	GACNNNNNGTC	1 cut(s)	187
EarI	CTCTTC	1 cut(s)	302
EciI	GGCGGA	3 cut(s)	54, 81, 437
EclXI	CGGCCG	1 cut(s)	439
Eco130I	CCWWGG	2 cut(s)	405, 952
Eco47I	GGWCC	2 cut(s)	830, 1152
Eco52I	CGGCCG	1 cut(s)	439
Eco57I	CTGAAG	1 cut(s)	326
Eco88I	CYCGRG	1 cut(s)	269
Eco91I	GGTNACC	1 cut(s)	947
EcoO109I	RGGNCCY	1 cut(s)	830
EcoO65I	GGTNACC	1 cut(s)	947
EcoRI	GAATTC	2 cut(s)	869, 1112
EcoRII	CCWGG	1 cut(s)	429
EcoT14I	CCWWGG	2 cut(s)	405, 952
EcoT22I	ATGCAT	1 cut(s)	1181
ErhI	CCWWGG	2 cut(s)	405, 952
FaeI	CATG	7 cut(s)	326, 806, 843, 1013, 1024, 1077, 1159
FalI	AAGNNNNNCTT	2 cut(s)	978, 1010
FaqI	GGGAC	4 cut(s)	643, 843, 1232, 1524
FatI	CATG	7 cut(s)	322, 802, 839, 1009, 1020, 1073, 1155
FauNDI	CATATG	1 cut(s)	886
FokI	GGATG	4 cut(s)	362, 614, 1182, 1247
FspBI	CTAG	4 cut(s)	465, 680, 1469, 1499
GsaI	CCCAGC	1 cut(s)	1523
GsuI	CTGGAG	2 cut(s)	413, 1479
HaeIII	GGCC	4 cut(s)	45, 404, 441, 637
HapII	CCGG	4 cut(s)	270, 818, 1040, 1122
HgaI	GACGC	2 cut(s)	218, 1484
Hin1I	GRCGYC	1 cut(s)	210
Hin1II	CATG	7 cut(s)	326, 806, 843, 1013, 1024, 1077, 1159
HindIII	AAGCTT	2 cut(s)	1556, 1577
HinfI	GANTC	7 cut(s)	140, 185, 265, 392, 1046, 1245, 1479
HpaII	CCGG	4 cut(s)	270, 818, 1040, 1122
HphI	GGTGA	5 cut(s)	124, 520, 941, 968, 1250
Hpy188III	TCNNGA	2 cut(s)	1040, 1341
Hpy99I	CGWCG	6 cut(s)	167, 185, 197, 200, 212, 218
HpyAV	CCTTC	7 cut(s)	121, 289, 331, 370, 374, 1225, 1497
HpyCH4III	ACNGT	3 cut(s)	898, 1003, 1412
HpyCH4V	TGCA	9 cut(s)	415, 595, 722, 914, 1073, 1179, 1211, 1475, 1541
HpyF3I	CTNAG	6 cut(s)	52, 246, 284, 505, 576, 1527
Hsp92I	GRCGYC	1 cut(s)	210
Hsp92II	CATG	7 cut(s)	326, 806, 843, 1013, 1024, 1077, 1159
Kpn2I	TCCGGA	1 cut(s)	1039
Kzo9I	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
LmnI	GCTCC	6 cut(s)	61, 86, 177, 432, 458, 1343
Lsp1109I	GCAGC	7 cut(s)	427, 430, 433, 436, 1146, 1195, 1510
LweI	GCATC	3 cut(s)	523, 581, 1346
MaeI	CTAG	4 cut(s)	465, 680, 1469, 1499
MaeIII	GTNAC	7 cut(s)	526, 641, 821, 947, 1256, 1306, 1325
MalI	GATC	6 cut(s)	503, 675, 765, 891, 1014, 1298
MbiI	CCGCTC	1 cut(s)	453
MboI	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
MboII	GAAGA	7 cut(s)	131, 289, 319, 343, 346, 353, 929
MflI	RGATCY	2 cut(s)	501, 673
MhlI	GDGCHC	1 cut(s)	1069
MlsI	TGGCCA	1 cut(s)	404
MluCI	AATT	3 cut(s)	869, 1112, 1435
MluNI	TGGCCA	1 cut(s)	404
MlyI	GAGTC	6 cut(s)	194, 259, 386, 1055, 1254, 1488
MmeI	TCCRAC	4 cut(s)	216, 985, 1405, 1462
Mox20I	TGGCCA	1 cut(s)	404
Mph1103I	ATGCAT	1 cut(s)	1181
MroI	TCCGGA	1 cut(s)	1039
MscI	TGGCCA	1 cut(s)	404
MseI	TTAA	5 cut(s)	845, 900, 1185, 1434, 1554
MslI	CAYNNNNRTG	5 cut(s)	533, 600, 954, 1084, 1218
Msp20I	TGGCCA	1 cut(s)	404
MspA1I	CMGCKG	2 cut(s)	438, 1523
MspCI	CTTAAG	1 cut(s)	1553
MspI	CCGG	4 cut(s)	270, 818, 1040, 1122
MspR9I	CCNGG	4 cut(s)	270, 271, 431, 819
Mva1269I	GAATGC	2 cut(s)	916, 1195
MvaI	CCWGG	1 cut(s)	431
MvnI	CGCG	2 cut(s)	1051, 1495
NciI	CCSGG	3 cut(s)	270, 271, 819
NdeI	CATATG	1 cut(s)	886
NdeII	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
NlaIII	CATG	7 cut(s)	326, 806, 843, 1013, 1024, 1077, 1159
NlaIV	GGNNCC	4 cut(s)	675, 831, 1038, 1491
NmeAIII	GCCGAG	1 cut(s)	71
NmuCI	GTSAC	7 cut(s)	526, 641, 821, 947, 1256, 1306, 1325
NotI	GCGGCCGC	1 cut(s)	439
NsiI	ATGCAT	1 cut(s)	1181
NspI	RCATGY	2 cut(s)	806, 1077
OliI	CACNNNNGTG	2 cut(s)	533, 954
PaeI	GCATGC	1 cut(s)	1077
PcsI	WCGNNNNNNNCGW	4 cut(s)	189, 204, 222, 351
PctI	GAATGC	2 cut(s)	916, 1195
PfeI	GAWTC	1 cut(s)	140
PflFI	GACNNNGTC	1 cut(s)	212
PfoI	TCCNGGA	1 cut(s)	429
PleI	GAGTC	6 cut(s)	193, 259, 386, 1054, 1253, 1487
PpsI	GAGTC	6 cut(s)	193, 259, 386, 1054, 1253, 1487
PpuMI	RGGWCCY	1 cut(s)	830
Psp5II	RGGWCCY	1 cut(s)	830
Psp6I	CCWGG	1 cut(s)	429
PspEI	GGTNACC	1 cut(s)	947
PspFI	CCCAGC	1 cut(s)	1519
PspGI	CCWGG	1 cut(s)	429
PspN4I	GGNNCC	4 cut(s)	675, 831, 1038, 1491
PspPI	GGNCC	3 cut(s)	636, 830, 1152
PspPPI	RGGWCCY	1 cut(s)	830
PstNI	CAGNNNCTG	1 cut(s)	1346
PsuI	RGATCY	2 cut(s)	501, 673
PsyI	GACNNNGTC	1 cut(s)	212
PvuII	CAGCTG	1 cut(s)	1523
RsaI	GTAC	1 cut(s)	793
RsaNI	GTAC	1 cut(s)	792
RseI	CAYNNNNRTG	5 cut(s)	533, 600, 954, 1084, 1218
SaqAI	TTAA	5 cut(s)	845, 900, 1185, 1434, 1554
Sau3AI	GATC	6 cut(s)	501, 673, 763, 889, 1012, 1296
Sau96I	GGNCC	3 cut(s)	636, 830, 1152
SchI	GAGTC	6 cut(s)	194, 259, 386, 1055, 1254, 1488
ScrFI	CCNGG	4 cut(s)	270, 271, 431, 819
SduI	GDGCHC	1 cut(s)	1069
SfaNI	GCATC	3 cut(s)	523, 581, 1346
SinI	GGWCC	2 cut(s)	830, 1152
SmaI	CCCGGG	1 cut(s)	271
SmiMI	CAYNNNNRTG	5 cut(s)	533, 600, 954, 1084, 1218
SmlI	CTYRAG	1 cut(s)	1553
SmoI	CTYRAG	1 cut(s)	1553
SphI	GCATGC	1 cut(s)	1077
Sse9I	AATT	3 cut(s)	869, 1112, 1435
SspMI	CTAG	4 cut(s)	465, 680, 1469, 1499
StyD4I	CCNGG	4 cut(s)	268, 269, 429, 817
StyI	CCWWGG	2 cut(s)	405, 952
TaaI	ACNGT	3 cut(s)	898, 1003, 1412
TaqI	TCGA	3 cut(s)	892, 1015, 1318
TaqII	GACCGA	1 cut(s)	216
TasI	AATT	3 cut(s)	869, 1112, 1435
TfiI	GAWTC	1 cut(s)	140
Tru1I	TTAA	5 cut(s)	845, 900, 1185, 1434, 1554
Tru9I	TTAA	5 cut(s)	845, 900, 1185, 1434, 1554
TscAI	CASTG	4 cut(s)	540, 638, 646, 1330
TseFI	GTSAC	7 cut(s)	526, 641, 821, 947, 1256, 1306, 1325
TseI	GCWGC	7 cut(s)	415, 418, 421, 424, 1159, 1208, 1523
Tsp45I	GTSAC	7 cut(s)	526, 641, 821, 947, 1256, 1306, 1325
TspDTI	ATGAA	5 cut(s)	17, 764, 873, 1125, 1269
TspGWI	ACGGA	2 cut(s)	997, 1052
TspMI	CCCGGG	1 cut(s)	269
TspRI	CASTG	4 cut(s)	540, 638, 646, 1330
Tth111I	GACNNNGTC	1 cut(s)	212
Vha464I	CTTAAG	1 cut(s)	1553
VpaK11BI	GGWCC	2 cut(s)	830, 1152
XapI	RAATTY	2 cut(s)	869, 1112
XceI	RCATGY	2 cut(s)	806, 1077
XmaI	CCCGGG	1 cut(s)	269
XspI	CTAG	4 cut(s)	465, 680, 1469, 1499
Zsp2I	ATGCAT	1 cut(s)	1181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.