Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

12260444 .. 12264662

Gene Structure 4219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032227

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1578 bp

ATGAACAGGAACAAGAAGCGAGGCGGCGGCGGCGGGGGAGGCTCAGCTCCCAAGGGCGGAAGAGGAGGAGACAGAAAGGGAGCTAAAAAAGGCAAGAGGCAGTCCTTCAAGGAAGACCCATTTTTCACCAACGAATCCAAGAAGCGGCTCAAATACGACGAGGACATTCGGAGCGACGAGTCGTCCGACGACGACCGAAACGACGCCGTCGGTAGTGGCGAGGAGGAGGAGGAGGCGGCTGAGGAGATTATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTTGGAGAGCATGAGAGGAAGAAGAAGGAAGACGAGGATGACGACAGCGATGACGACAGCGAGAAGGAAGGGGACAGAGACTCGCTTGTGGCCAAGGTTTTGCAGCAGCAACAGCTCCAGGATAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCAGAAGCTACTGATGAGTTTCGGATCTTAGTGAAGCATCGCCAATCTGTCACTGCAGTGGTTCTCTCTGACGATGATGTCAGAGGTTTTTCAGCATCTAAGGACGGCGCAATTATGCATTGGGATGTAGATAGTGGGAAAAAAGAGAAGTATCAGTGGCCCAGTGACGAAGTATTGAAGTCCCACGGGGTCAAGGATCCACTAGGTAGAGCGACAAAGCATAGCAAACAAATGTTAGCACTTGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATATTCATTTGTGGGATACTCGTACCAGACAGCATGTTCAGGCTTTGCCGGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCAGAACTCTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGCAGAAGATAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCAGAGGAGTCGCGTTTAGTATTCCGTGCCCCTGCATGCTCATTGGAGTGTTGTTCTTTTATTAGCAATGATGAATTCTTATCCGGCTGTGATAATGGAAGTGTTGAGCTTTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTTTGTTAGCTGCAAACAAAGATGTTGAACAGAAGGATGGTGATAGAGTCCCCAATGGTCACATAGAAAGCTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGTAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATTCAGCCCTTACAGAGTCTCCCCGTGGTTGGATTTGTTAATTCTTTAGCTATTGCAAAATCTGGAGGCTTTCTAGTTGCAGGAGTCGGAAAGGAACCGCGTCTAGGACGGTGGGGACTTATCCCAGCTGCTCAGAATGGAGTTGCAATCCACACCCTTAAGCTTTCGTCAGATACAAATAAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

526

Amino Acids

58.09

Weight (kDa)

6.93

Isoelectric Point (pI)

52.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	160 - 346	1.1e-16	CDC20/Fizzy WD40 domain
WDR55	PF24796	161 - 341	3.4e-11	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	190 - 340	2.3e-06	WDR36/Utp21 first beta-propeller
NBCH_WD40	PF20426	202 - 326	1.8e-06	Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd	PF25047	210 - 340	1.2e-12	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	224 - 382	6.2e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	225 - 284	4.2e-10	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	227 - 342	6.5e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	229 - 260	3.3e-06	WD domain, G-beta repeat
Beta-prop_EML	PF23409	229 - 397	5.8e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	229 - 389	6.2e-23	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	230 - 384	1.2e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	230 - 344	4.9e-24	WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	231 - 347	4e-09	MABP1/WDR62 second WD40 domain
WD40_MABP1-WDR62_1st	PF24780	232 - 307	2.5e-06	MABP1/WDR62 first WD40 domain
WD40_Prp19	PF24814	233 - 391	2.4e-19	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	234 - 347	1.6e-15	WDR3 second beta-propeller domain
WD40	PF00400	266 - 302	3.1e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	273 - 346	4.9e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	548
AccBSI	CCGCTC	1 cut(s)	447
AccII	CGCG	2 cut(s)	1045, 1489
AclWI	GGATC	4 cut(s)	503, 662, 675, 1298
AcoI	YGGCCR	2 cut(s)	396, 433
AcsI	RAATTY	1 cut(s)	1106
AcuI	CTGAAG	1 cut(s)	320
AcyI	GRCGYC	1 cut(s)	204
AfaI	GTAC	1 cut(s)	787
AfiI	CCNNNNNNNGG	4 cut(s)	56, 144, 1418, 1493
AflII	CTTAAG	1 cut(s)	1547
AgsI	TTSAA	5 cut(s)	109, 649, 1027, 1220, 1373
AhdI	GACNNNNNGTC	1 cut(s)	181
AjnI	CCWGG	1 cut(s)	423
AleI	CACNNNNGTG	2 cut(s)	527, 948
Alw26I	GTCTC	4 cut(s)	63, 251, 378, 1412
AlwI	GGATC	4 cut(s)	503, 662, 675, 1298
AlwNI	CAGNNNCTG	1 cut(s)	1340
Ama87I	CYCGRG	1 cut(s)	263
AoxI	GGCC	3 cut(s)	396, 433, 629
ApeKI	GCWGC	5 cut(s)	409, 412, 1153, 1202, 1517
ApoI	RAATTY	1 cut(s)	1106
AspLEI	GCGC	1 cut(s)	581
AspS9I	GGNCC	3 cut(s)	630, 824, 1146
AsuC2I	CCSGG	3 cut(s)	264, 265, 813
AsuHPI	GGTGA	4 cut(s)	118, 935, 962, 1244
AvaI	CYCGRG	1 cut(s)	263
AvaII	GGWCC	2 cut(s)	824, 1146
BaeGI	GKGCMC	1 cut(s)	1063
BalI	TGGCCA	1 cut(s)	398
BamHI	GGATCC	1 cut(s)	667
BbsI	GAAGAC	2 cut(s)	120, 342
BbvCI	CCTCAGC	2 cut(s)	240, 278
BbvI	GCAGC	5 cut(s)	421, 424, 1140, 1189, 1504
BccI	CCATC	2 cut(s)	722, 1223
BceAI	ACGGC	3 cut(s)	191, 592, 1001
BciT130I	CCWGG	1 cut(s)	425
BciVI	GTATCC	1 cut(s)	772
BcnI	CCSGG	3 cut(s)	264, 265, 813
BcoDI	GTCTC	4 cut(s)	63, 251, 378, 1412
BfaI	CTAG	4 cut(s)	459, 674, 1463, 1493
BfmI	CTRYAG	1 cut(s)	525
BfrI	CTTAAG	1 cut(s)	1547
BfuI	GTATCC	1 cut(s)	772
BlpI	GCTNAGC	1 cut(s)	43
Bme1390I	CCNGG	4 cut(s)	264, 265, 425, 813
Bme18I	GGWCC	2 cut(s)	824, 1146
BmeRI	GACNNNNNGTC	1 cut(s)	181
BmeT110I	CYCGRG	1 cut(s)	263
BmgT120I	GGNCC	3 cut(s)	630, 824, 1146
BmiI	GGNNCC	4 cut(s)	669, 825, 1032, 1485
BmrFI	CCNGG	4 cut(s)	264, 265, 425, 813
BmrI	ACTGGG	1 cut(s)	627
BmsI	GCATC	3 cut(s)	517, 575, 1340
BmuI	ACTGGG	1 cut(s)	627
BpiI	GAAGAC	2 cut(s)	120, 342
BpmI	CTGGAG	2 cut(s)	407, 1473
Bpu10I	CCTNAGC	2 cut(s)	240, 278
Bpu1102I	GCTNAGC	1 cut(s)	43
BpuMI	CCSGG	3 cut(s)	264, 265, 813
BsaHI	GRCGYC	1 cut(s)	204
BsaJI	CCNNGG	7 cut(s)	51, 263, 264, 399, 655, 946, 1413
BsaXI	ACNNNNNCTCC	2 cut(s)	1392, 1422
Bsc4I	CCNNNNNNNGG	4 cut(s)	56, 144, 1418, 1493
Bse1I	ACTGG	1 cut(s)	633
Bse3DI	GCAATG	3 cut(s)	450, 1105, 1390
BseBI	CCWGG	1 cut(s)	425
BseDI	CCNNGG	7 cut(s)	51, 263, 264, 399, 655, 946, 1413
BseGI	GGATG	4 cut(s)	349, 601, 1189, 1234
BseLI	CCNNNNNNNGG	4 cut(s)	56, 144, 1418, 1493
BseMI	GCAATG	3 cut(s)	450, 1105, 1390
BseMII	CTCAG	4 cut(s)	57, 231, 269, 1535
BseNI	ACTGG	1 cut(s)	633
BseRI	GAGGAG	9 cut(s)	78, 81, 236, 239, 242, 245, 257, 269, 1052
BseSI	GKGCMC	1 cut(s)	1063
BseX3I	CGGCCG	1 cut(s)	433
BseXI	GCAGC	5 cut(s)	421, 424, 1140, 1189, 1504
BseYI	CCCAGC	1 cut(s)	1513
Bsh1236I	CGCG	2 cut(s)	1045, 1489
Bsh1285I	CGRYCG	2 cut(s)	196, 436
BshFI	GGCC	3 cut(s)	398, 435, 631
BsiEI	CGRYCG	2 cut(s)	196, 436
BsiHKCI	CYCGRG	1 cut(s)	263
BsiSI	CCGG	3 cut(s)	264, 812, 1116
BslFI	GGGAC	5 cut(s)	392, 637, 837, 1226, 1518
BslI	CCNNNNNNNGG	4 cut(s)	56, 144, 1418, 1493
BsmAI	GTCTC	4 cut(s)	63, 251, 378, 1412
BsmFI	GGGAC	5 cut(s)	392, 637, 837, 1226, 1518
BsmI	GAATGC	2 cut(s)	910, 1189
BsnI	GGCC	3 cut(s)	398, 435, 631
BsoBI	CYCGRG	1 cut(s)	263
Bsp1286I	GDGCHC	1 cut(s)	1063
Bsp143I	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
Bsp1720I	GCTNAGC	1 cut(s)	43
BspANI	GGCC	3 cut(s)	398, 435, 631
BspCNI	CTCAG	4 cut(s)	56, 232, 270, 1534
BspFNI	CGCG	2 cut(s)	1045, 1489
BspLI	GGNNCC	4 cut(s)	669, 825, 1032, 1485
BspMAI	CTGCAG	1 cut(s)	529
BspPI	GGATC	4 cut(s)	503, 662, 675, 1298
BspTI	CTTAAG	1 cut(s)	1547
BsrBI	CCGCTC	1 cut(s)	447
BsrDI	GCAATG	3 cut(s)	450, 1105, 1390
BsrI	ACTGG	1 cut(s)	633
BssECI	CCNNGG	7 cut(s)	51, 263, 264, 399, 655, 946, 1413
BssMI	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
BssNI	GRCGYC	1 cut(s)	204
BssT1I	CCWWGG	3 cut(s)	51, 399, 946
Bst2UI	CCWGG	1 cut(s)	425
Bst4CI	ACNGT	3 cut(s)	892, 997, 1500
Bst6I	CTCTTC	2 cut(s)	55, 296
BstACI	GRCGYC	1 cut(s)	204
BstAFI	CTTAAG	1 cut(s)	1547
BstC8I	GCNNGC	1 cut(s)	1069
BstDEI	CTNAG	6 cut(s)	43, 240, 278, 499, 570, 1521
BstDSI	CCRYGG	2 cut(s)	655, 1413
BstEII	GGTNACC	1 cut(s)	941
BstF5I	GGATG	4 cut(s)	349, 601, 1189, 1234
BstFNI	CGCG	2 cut(s)	1045, 1489
BstHHI	GCGC	1 cut(s)	581
BstKTI	GATC	6 cut(s)	498, 670, 760, 886, 1009, 1293
BstMAI	GTCTC	4 cut(s)	63, 251, 378, 1412
BstMBI	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
BstMCI	CGRYCG	2 cut(s)	196, 436
BstMWI	GCNNNNNNNGC	8 cut(s)	30, 39, 313, 418, 441, 444, 1162, 1337
BstNI	CCWGG	1 cut(s)	425
BstNSI	RCATGY	2 cut(s)	800, 1071
BstPI	GGTNACC	1 cut(s)	941
BstSCI	CCNGG	4 cut(s)	262, 263, 423, 811
BstSFI	CTRYAG	1 cut(s)	525
BstSLI	GKGCMC	1 cut(s)	1063
BstUI	CGCG	2 cut(s)	1045, 1489
BstV1I	GCAGC	5 cut(s)	421, 424, 1140, 1189, 1504
BstV2I	GAAGAC	2 cut(s)	120, 342
BstX2I	RGATCY	2 cut(s)	495, 667
BstYI	RGATCY	2 cut(s)	495, 667
BstZI	CGGCCG	1 cut(s)	433
BsuI	GTATCC	1 cut(s)	772
BsuRI	GGCC	3 cut(s)	398, 435, 631
BtgI	CCRYGG	2 cut(s)	655, 1413
BtgZI	GCGATG	2 cut(s)	369, 494
BtsCI	GGATG	4 cut(s)	349, 601, 1189, 1234
BtsI	GCAGTG	3 cut(s)	522, 534, 1324
BtsIMutI	CAGTG	5 cut(s)	522, 534, 632, 640, 1324
Cac8I	GCNNGC	1 cut(s)	1069
CaiI	CAGNNNCTG	1 cut(s)	1340
CciNI	GCGGCCGC	1 cut(s)	433
CfoI	GCGC	1 cut(s)	581
Cfr13I	GGNCC	3 cut(s)	630, 824, 1146
Cfr9I	CCCGGG	1 cut(s)	263
CseI	GACGC	2 cut(s)	212, 1478
Csp6I	GTAC	1 cut(s)	786
CviAII	CATG	7 cut(s)	317, 797, 834, 1004, 1015, 1068, 1150
CviQI	GTAC	1 cut(s)	786
DdeI	CTNAG	6 cut(s)	43, 240, 278, 499, 570, 1521
DpnI	GATC	6 cut(s)	497, 669, 759, 885, 1008, 1292
DpnII	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
DrdI	GACNNNNNNGTC	1 cut(s)	548
DriI	GACNNNNNGTC	1 cut(s)	181
DseDI	GACNNNNNNGTC	1 cut(s)	548
EaeI	YGGCCR	2 cut(s)	396, 433
EagI	CGGCCG	1 cut(s)	433
Eam1104I	CTCTTC	2 cut(s)	55, 296
Eam1105I	GACNNNNNGTC	1 cut(s)	181
EarI	CTCTTC	2 cut(s)	55, 296
EciI	GGCGGA	2 cut(s)	72, 431
EclXI	CGGCCG	1 cut(s)	433
Eco130I	CCWWGG	3 cut(s)	51, 399, 946
Eco47I	GGWCC	2 cut(s)	824, 1146
Eco52I	CGGCCG	1 cut(s)	433
Eco57I	CTGAAG	1 cut(s)	320
Eco88I	CYCGRG	1 cut(s)	263
Eco91I	GGTNACC	1 cut(s)	941
EcoO109I	RGGNCCY	1 cut(s)	824
EcoO65I	GGTNACC	1 cut(s)	941
EcoRI	GAATTC	1 cut(s)	1106
EcoRII	CCWGG	1 cut(s)	423
EcoT14I	CCWWGG	3 cut(s)	51, 399, 946
EcoT22I	ATGCAT	2 cut(s)	591, 1175
ErhI	CCWWGG	3 cut(s)	51, 399, 946
FaeI	CATG	7 cut(s)	320, 800, 837, 1007, 1018, 1071, 1153
FalI	AAGNNNNNCTT	2 cut(s)	972, 1004
FaqI	GGGAC	5 cut(s)	392, 637, 837, 1226, 1518
FatI	CATG	7 cut(s)	316, 796, 833, 1003, 1014, 1067, 1149
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	880
FokI	GGATG	4 cut(s)	356, 608, 1176, 1241
FspBI	CTAG	4 cut(s)	459, 674, 1463, 1493
GlaI	GCGC	1 cut(s)	580
GsaI	CCCAGC	1 cut(s)	1517
GsuI	CTGGAG	2 cut(s)	407, 1473
HaeIII	GGCC	3 cut(s)	398, 435, 631
HapII	CCGG	3 cut(s)	264, 812, 1116
HgaI	GACGC	2 cut(s)	212, 1478
HhaI	GCGC	1 cut(s)	581
Hin1I	GRCGYC	1 cut(s)	204
Hin1II	CATG	7 cut(s)	320, 800, 837, 1007, 1018, 1071, 1153
Hin6I	GCGC	1 cut(s)	579
HinP1I	GCGC	1 cut(s)	579
HindIII	AAGCTT	2 cut(s)	1550, 1571
HinfI	GANTC	8 cut(s)	134, 179, 259, 386, 1040, 1239, 1405, 1473
HpaII	CCGG	3 cut(s)	264, 812, 1116
HphI	GGTGA	4 cut(s)	118, 935, 962, 1244
Hpy188III	TCNNGA	3 cut(s)	1034, 1335, 1452
Hpy99I	CGWCG	6 cut(s)	161, 179, 191, 194, 206, 212
HpyAV	CCTTC	6 cut(s)	115, 283, 325, 364, 368, 1219
HpyCH4III	ACNGT	3 cut(s)	892, 997, 1500
HpyF10VI	GCNNNNNNNGC	8 cut(s)	30, 39, 313, 418, 441, 444, 1162, 1337
HpyF3I	CTNAG	6 cut(s)	43, 240, 278, 499, 570, 1521
Hsp92I	GRCGYC	1 cut(s)	204
Hsp92II	CATG	7 cut(s)	320, 800, 837, 1007, 1018, 1071, 1153
HspAI	GCGC	1 cut(s)	579
Kzo9I	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
LmnI	GCTCC	6 cut(s)	52, 80, 171, 426, 452, 1337
Lsp1109I	GCAGC	5 cut(s)	421, 424, 1140, 1189, 1504
LweI	GCATC	3 cut(s)	517, 575, 1340
MaeI	CTAG	4 cut(s)	459, 674, 1463, 1493
MaeIII	GTNAC	7 cut(s)	520, 635, 815, 941, 1250, 1300, 1319
MalI	GATC	6 cut(s)	497, 669, 759, 885, 1008, 1292
MbiI	CCGCTC	1 cut(s)	447
MboI	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
MboII	GAAGA	8 cut(s)	72, 125, 283, 313, 337, 340, 347, 923
MflI	RGATCY	2 cut(s)	495, 667
MhlI	GDGCHC	1 cut(s)	1063
MlsI	TGGCCA	1 cut(s)	398
MluCI	AATT	3 cut(s)	582, 1106, 1429
MluNI	TGGCCA	1 cut(s)	398
MlyI	GAGTC	7 cut(s)	188, 253, 380, 1049, 1248, 1414, 1482
MmeI	TCCRAC	4 cut(s)	210, 979, 1399, 1456
Mox20I	TGGCCA	1 cut(s)	398
Mph1103I	ATGCAT	2 cut(s)	591, 1175
MscI	TGGCCA	1 cut(s)	398
MseI	TTAA	5 cut(s)	839, 894, 1179, 1428, 1548
MslI	CAYNNNNRTG	4 cut(s)	527, 594, 948, 1078
Msp20I	TGGCCA	1 cut(s)	398
MspA1I	CMGCKG	1 cut(s)	1517
MspCI	CTTAAG	1 cut(s)	1547
MspI	CCGG	3 cut(s)	264, 812, 1116
MspR9I	CCNGG	4 cut(s)	264, 265, 425, 813
Mva1269I	GAATGC	2 cut(s)	910, 1189
MvaI	CCWGG	1 cut(s)	425
MvnI	CGCG	2 cut(s)	1045, 1489
MwoI	GCNNNNNNNGC	8 cut(s)	30, 39, 313, 418, 441, 444, 1162, 1337
NciI	CCSGG	3 cut(s)	264, 265, 813
NdeI	CATATG	1 cut(s)	880
NdeII	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
NlaIII	CATG	7 cut(s)	320, 800, 837, 1007, 1018, 1071, 1153
NlaIV	GGNNCC	4 cut(s)	669, 825, 1032, 1485
NmuCI	GTSAC	7 cut(s)	520, 635, 815, 941, 1250, 1300, 1319
NotI	GCGGCCGC	1 cut(s)	433
NsiI	ATGCAT	2 cut(s)	591, 1175
NspI	RCATGY	2 cut(s)	800, 1071
OliI	CACNNNNGTG	2 cut(s)	527, 948
PaeI	GCATGC	1 cut(s)	1071
PcsI	WCGNNNNNNNCGW	4 cut(s)	183, 198, 216, 345
PctI	GAATGC	2 cut(s)	910, 1189
PfeI	GAWTC	1 cut(s)	134
PflFI	GACNNNGTC	1 cut(s)	206
PfoI	TCCNGGA	1 cut(s)	423
PleI	GAGTC	7 cut(s)	187, 253, 380, 1048, 1247, 1413, 1481
PpsI	GAGTC	7 cut(s)	187, 253, 380, 1048, 1247, 1413, 1481
PpuMI	RGGWCCY	1 cut(s)	824
Psp5II	RGGWCCY	1 cut(s)	824
Psp6I	CCWGG	1 cut(s)	423
PspEI	GGTNACC	1 cut(s)	941
PspFI	CCCAGC	1 cut(s)	1513
PspGI	CCWGG	1 cut(s)	423
PspN4I	GGNNCC	4 cut(s)	669, 825, 1032, 1485
PspPI	GGNCC	3 cut(s)	630, 824, 1146
PspPPI	RGGWCCY	1 cut(s)	824
PstI	CTGCAG	1 cut(s)	529
PstNI	CAGNNNCTG	1 cut(s)	1340
PsuI	RGATCY	2 cut(s)	495, 667
PsyI	GACNNNGTC	1 cut(s)	206
PvuII	CAGCTG	1 cut(s)	1517
RsaI	GTAC	1 cut(s)	787
RsaNI	GTAC	1 cut(s)	786
RseI	CAYNNNNRTG	4 cut(s)	527, 594, 948, 1078
SaqAI	TTAA	5 cut(s)	839, 894, 1179, 1428, 1548
Sau3AI	GATC	6 cut(s)	495, 667, 757, 883, 1006, 1290
Sau96I	GGNCC	3 cut(s)	630, 824, 1146
SchI	GAGTC	7 cut(s)	188, 253, 380, 1049, 1248, 1414, 1482
ScrFI	CCNGG	4 cut(s)	264, 265, 425, 813
SduI	GDGCHC	1 cut(s)	1063
SfaNI	GCATC	3 cut(s)	517, 575, 1340
SfcI	CTRYAG	1 cut(s)	525
SinI	GGWCC	2 cut(s)	824, 1146
SmaI	CCCGGG	1 cut(s)	265
SmiMI	CAYNNNNRTG	4 cut(s)	527, 594, 948, 1078
SmlI	CTYRAG	1 cut(s)	1547
SmoI	CTYRAG	1 cut(s)	1547
SphI	GCATGC	1 cut(s)	1071
Sse9I	AATT	3 cut(s)	582, 1106, 1429
SspMI	CTAG	4 cut(s)	459, 674, 1463, 1493
StyD4I	CCNGG	4 cut(s)	262, 263, 423, 811
StyI	CCWWGG	3 cut(s)	51, 399, 946
TaaI	ACNGT	3 cut(s)	892, 997, 1500
TaqI	TCGA	3 cut(s)	886, 1009, 1312
TaqII	GACCGA	1 cut(s)	210
TasI	AATT	3 cut(s)	582, 1106, 1429
TauI	GCSGC	9 cut(s)	27, 30, 33, 148, 239, 435, 438, 447, 1331
TfiI	GAWTC	1 cut(s)	134
Tru1I	TTAA	5 cut(s)	839, 894, 1179, 1428, 1548
Tru9I	TTAA	5 cut(s)	839, 894, 1179, 1428, 1548
TscAI	CASTG	5 cut(s)	529, 534, 632, 640, 1324
TseFI	GTSAC	7 cut(s)	520, 635, 815, 941, 1250, 1300, 1319
TseI	GCWGC	5 cut(s)	409, 412, 1153, 1202, 1517
Tsp45I	GTSAC	7 cut(s)	520, 635, 815, 941, 1250, 1300, 1319
TspDTI	ATGAA	5 cut(s)	17, 758, 867, 1119, 1263
TspGWI	ACGGA	2 cut(s)	991, 1046
TspMI	CCCGGG	1 cut(s)	263
TspRI	CASTG	5 cut(s)	529, 534, 632, 640, 1324
Tth111I	GACNNNGTC	1 cut(s)	206
Vha464I	CTTAAG	1 cut(s)	1547
VpaK11BI	GGWCC	2 cut(s)	824, 1146
XapI	RAATTY	1 cut(s)	1106
XceI	RCATGY	2 cut(s)	800, 1071
XmaI	CCCGGG	1 cut(s)	263
XspI	CTAG	4 cut(s)	459, 674, 1463, 1493
Zsp2I	ATGCAT	2 cut(s)	591, 1175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.