Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

34649597 .. 34653690

Gene Structure 4094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1254000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGAAGAACAAGAAGAGAGGCGGCGGCTCAGCTCCCAAGGGACCCAAAAAGGGCAAGGCTTTCTCTCTCGACGAAGATCCCTTCTTCGGCACCGAATCCAAGAAGCGCCGGAAAGTCGAGCGCGATGAGATTGAGAGCGAATCCGACGACGACTACGGGGCCGTCGGGTCGGACGGCGAGGGCGGCGGAGAGGAGGCCGAGGAGGAGGGGGAGGCTATAGTGGAAACGGCAGACGAGAAGAGGAATAGGCTTGCTCATGAGTTCTTGGAGAACACTCGGGAGCTGCTGAGGAGGGCCAAGGAAGATGATGAGGATGATGATAGCGGTGAAGAGGGTGTGAAGGAAGGCGAGATGGACTCGCTTGTTGGCAAGATGTTGCAGCAGCAGCAGCTCGAGGACAGCGGCCGAGCTCGCCGTGCCATTGCTTCTAGGGTTAAAAAGCCAGAATCTACTGATGAATTTCGGATATTAGTGAAGCATCGACAAACTGTTACTGCTGTGGTTCTGTCCGAGAATGACCTCAGGGGCTTTTCAGCTTCTAAGGATGGCACCATTCTACATTGGGATGTAGATAGTGGGAAAAGTGAAAAGTATCTATGGCCTAGTGATGAAGTACTTAAGTCTCATGGGGTCAAGGATCCACAAGGTCGAGCTAAAAAGCATAGTAAACACGTGTTAGCACTAGCAGTTAGCTCTGATGGTCGGTATTTGGCAAGTGGGGGCTTAGATCGCCATGTTCATTTGTGGGATACTCGTACACGAGAGCATATTCTGGCTTTTCCAGGTCACAGGGGCCCCGTTTCATGTTTAACTTTCAGGCAAGGGTCTTCAGAACTTTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGTAGAAGATAGAGCTTACATAAACACATTATTTGGTCATCAAGGGGAAGTATTAAGTATTGATTGCCTACGGAAAGAAAGGGTATTAACTGTTGGACATGATCGAAGCATGCAGTTATTTAAGGTCCCAGAGGAGTCACGTTTAGTATTCCGTGCCCCTGCATCTTCATTGGAGTGTTGTTGCTTTATTAGCAATGACGAATTCTTATCCGGCTCTGATGATGGAAGTGTTGAGCTTTGGAACATGGTGCGGAAGAAGCCTGTTTGCATAGTTAAGAATGCTCATCCTTTTTTGGCTGCACACAAAGAAGTCGAACAAAAGGATGGTGATAGAATCCCAAATGGTCACATAGAAAATGGTAACCACACTTCTGAAAGCTTTGATTGTTCATCAACCAATTCTTGGATCAGTTCAGTCACTGTTTGTAGAGGCAGTGACCTCGCTGCATCAGGAGCTGGTAATGGCTCTGTTCGATTATGGGCTATTGAAAGTGAGAGCAGAGTGCTTCAGCCGTTACACAACATCCCATTGGTTGGATTTGTGAATTCTTTAGCTTTTGCAAAATCTGGACGGTTTCTGGTTGCAGGAGTTGGGAAGGAACCTCGTCTAGGAAGGTGGGGACATAACCCAGCTGCTCGGAACGGAATCGCAATCCATTCCATTAAGCTTTCATCAGAAGATACAAACACTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

524

Amino Acids

57.81

Weight (kDa)

6.06

Isoelectric Point (pI)

46.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	150 - 372	2.5e-19	CDC20/Fizzy WD40 domain
WDR55	PF24796	151 - 385	1.4e-16	WDR55
Beta-prop_EML_2	PF23414	178 - 332	2.2e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	180 - 331	8.3e-08	WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd	PF25047	199 - 331	2.3e-15	TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	217 - 334	5.9e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	217 - 380	3.7e-25	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	217 - 300	9.5e-16	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	219 - 336	5.9e-06	CAF1B/HIR1 beta-propeller domain
WD40	PF00400	220 - 250	2.9e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	220 - 334	2.5e-24	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	220 - 387	4.7e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd	PF25172	221 - 337	3.9e-14	WDR3 second beta-propeller domain
EIF3I	PF24805	221 - 301	7.8e-07	EIF3I
WD40_Gbeta	PF25391	221 - 375	2.7e-14	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	222 - 380	1e-18	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	223 - 331	2.2e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	257 - 292	5.9e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	263 - 379	6.2e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	89, 548
AccB7I	CCANNNNNTGG	2 cut(s)	1278, 1409
AccII	CGCG	1 cut(s)	123
AciI	CCGC	7 cut(s)	21, 24, 183, 186, 324, 402, 1126
AclWI	GGATC	4 cut(s)	71, 632, 645, 1289
AcoI	YGGCCR	1 cut(s)	403
AcsI	RAATTY	3 cut(s)	458, 1076, 1420
AcuI	CTGAAG	2 cut(s)	813, 1367
AcvI	CACGTG	1 cut(s)	673
AfaI	GTAC	2 cut(s)	615, 757
AfiI	CCNNNNNNNGG	5 cut(s)	50, 86, 1278, 1409, 1484
AflII	CTTAAG	1 cut(s)	617
AflIII	ACRYGT	2 cut(s)	670, 672
AgsI	TTSAA	1 cut(s)	1364
AjnI	CCWGG	1 cut(s)	781
Alw21I	GWGCWC	1 cut(s)	412
Alw26I	GTCTC	1 cut(s)	627
AlwI	GGATC	4 cut(s)	71, 632, 645, 1289
AlwNI	CAGNNNCTG	2 cut(s)	1295, 1331
Ama87I	CYCGRG	2 cut(s)	276, 392
AoxI	GGCC	6 cut(s)	159, 195, 294, 403, 599, 793
ApaI	GGGCCC	1 cut(s)	797
ApeKI	GCWGC	8 cut(s)	283, 379, 382, 385, 388, 1172, 1319, 1508
ApoI	RAATTY	3 cut(s)	458, 1076, 1420
AspLEI	GCGC	2 cut(s)	108, 123
AspS9I	GGNCC	6 cut(s)	41, 159, 294, 793, 794, 1000
AsuHPI	GGTGA	2 cut(s)	338, 1214
AvaI	CYCGRG	2 cut(s)	276, 392
AvaII	GGWCC	2 cut(s)	41, 1000
AxyI	CCTNAGG	1 cut(s)	521
BaeGI	GKGCMC	2 cut(s)	797, 1033
BamHI	GGATCC	1 cut(s)	637
BanI	GGYRCC	2 cut(s)	89, 548
BanII	GRGCYC	2 cut(s)	412, 797
BarI	GAAGNNNNNNTAC	2 cut(s)	1228, 1260
BauI	CACGAG	1 cut(s)	759
BbrPI	CACGTG	1 cut(s)	673
BbsI	GAAGAC	1 cut(s)	819
Bbv12I	GWGCWC	1 cut(s)	412
BbvCI	CCTCAGC	1 cut(s)	287
BbvI	GCAGC	8 cut(s)	270, 391, 394, 397, 400, 1159, 1306, 1495
BccI	CCATC	5 cut(s)	346, 539, 692, 1091, 1193
BceAI	ACGGC	5 cut(s)	146, 190, 243, 399, 1372
BcgI	CGANNNNNNTGC	2 cut(s)	1297, 1331
BciT130I	CCWGG	1 cut(s)	783
BciVI	GTATCC	1 cut(s)	742
BcoDI	GTCTC	1 cut(s)	627
BfaI	CTAG	4 cut(s)	429, 603, 683, 1484
BfmI	CTRYAG	1 cut(s)	216
BfoI	RGCGCY	1 cut(s)	109
BfrI	CTTAAG	1 cut(s)	617
BfuI	GTATCC	1 cut(s)	742
BlpI	GCTNAGC	1 cut(s)	28
BmcAI	AGTACT	1 cut(s)	615
Bme1390I	CCNGG	1 cut(s)	783
Bme18I	GGWCC	2 cut(s)	41, 1000
BmeT110I	CYCGRG	2 cut(s)	276, 392
BmgT120I	GGNCC	6 cut(s)	41, 159, 294, 793, 794, 1000
BmrFI	CCNGG	1 cut(s)	783
BmsI	GCATC	3 cut(s)	487, 1046, 1331
BpiI	GAAGAC	1 cut(s)	819
BplI	GAGNNNNNCTC	2 cut(s)	341, 373
Bpu10I	CCTNAGC	1 cut(s)	287
Bpu1102I	GCTNAGC	1 cut(s)	28
BsaAI	YACGTR	1 cut(s)	673
BsaJI	CCNNGG	3 cut(s)	36, 198, 297
Bsc4I	CCNNNNNNNGG	5 cut(s)	50, 86, 1278, 1409, 1484
Bse21I	CCTNAGG	1 cut(s)	521
Bse3DI	GCAATG	2 cut(s)	420, 1075
BseBI	CCWGG	1 cut(s)	783
BseDI	CCNNGG	3 cut(s)	36, 198, 297
BseGI	GGATG	6 cut(s)	319, 550, 571, 1159, 1204, 1398
BseLI	CCNNNNNNNGG	5 cut(s)	50, 86, 1278, 1409, 1484
BseMI	GCAATG	2 cut(s)	420, 1075
BseMII	CTCAG	3 cut(s)	42, 278, 535
BseRI	GAGGAG	5 cut(s)	206, 215, 218, 304, 1022
BseSI	GKGCMC	2 cut(s)	797, 1033
BseX3I	CGGCCG	1 cut(s)	403
BseXI	GCAGC	8 cut(s)	270, 391, 394, 397, 400, 1159, 1306, 1495
BseYI	CCCAGC	1 cut(s)	1504
BsgI	GTGCAG	1 cut(s)	1158
Bsh1236I	CGCG	1 cut(s)	123
Bsh1285I	CGRYCG	1 cut(s)	406
BshFI	GGCC	6 cut(s)	161, 197, 296, 405, 601, 795
BshNI	GGYRCC	2 cut(s)	89, 548
BsiEI	CGRYCG	1 cut(s)	406
BsiHKAI	GWGCWC	1 cut(s)	412
BsiHKCI	CYCGRG	2 cut(s)	276, 392
BsiSI	CCGG	2 cut(s)	109, 1086
BslFI	GGGAC	3 cut(s)	54, 986, 1509
BslI	CCNNNNNNNGG	5 cut(s)	50, 86, 1278, 1409, 1484
BsmAI	GTCTC	1 cut(s)	627
BsmFI	GGGAC	3 cut(s)	54, 986, 1509
BsmI	GAATGC	1 cut(s)	1159
BsnI	GGCC	6 cut(s)	161, 197, 296, 405, 601, 795
BsoBI	CYCGRG	2 cut(s)	276, 392
Bsp120I	GGGCCC	1 cut(s)	793
Bsp1286I	GDGCHC	3 cut(s)	412, 797, 1033
Bsp143I	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
Bsp1720I	GCTNAGC	1 cut(s)	28
BspACI	CCGC	7 cut(s)	21, 24, 183, 186, 324, 402, 1126
BspANI	GGCC	6 cut(s)	161, 197, 296, 405, 601, 795
BspCNI	CTCAG	3 cut(s)	41, 279, 534
BspFNI	CGCG	1 cut(s)	123
BspHI	TCATGA	1 cut(s)	256
BspPI	GGATC	4 cut(s)	71, 632, 645, 1289
BspT107I	GGYRCC	2 cut(s)	89, 548
BspTI	CTTAAG	1 cut(s)	617
BsrDI	GCAATG	2 cut(s)	420, 1075
BssECI	CCNNGG	3 cut(s)	36, 198, 297
BssMI	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
BssSI	CACGAG	1 cut(s)	759
BssT1I	CCWWGG	2 cut(s)	36, 297
Bst2BI	CACGAG	1 cut(s)	759
Bst2UI	CCWGG	1 cut(s)	783
Bst4CI	ACNGT	5 cut(s)	490, 862, 967, 1297, 1449
Bst6I	CTCTTC	3 cut(s)	8, 233, 324
BstAFI	CTTAAG	1 cut(s)	617
BstBAI	YACGTR	1 cut(s)	673
BstC8I	GCNNGC	3 cut(s)	252, 412, 986
BstDEI	CTNAG	5 cut(s)	28, 287, 521, 540, 724
BstEII	GGTNACC	1 cut(s)	1235
BstENI	CCTNNNNNAGG	1 cut(s)	1482
BstF5I	GGATG	6 cut(s)	319, 550, 571, 1159, 1204, 1398
BstFNI	CGCG	1 cut(s)	123
BstH2I	RGCGCY	1 cut(s)	109
BstHHI	GCGC	2 cut(s)	108, 123
BstKTI	GATC	6 cut(s)	79, 640, 730, 856, 979, 1284
BstMAI	GTCTC	1 cut(s)	627
BstMBI	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
BstMCI	CGRYCG	1 cut(s)	406
BstMWI	GCNNNNNNNGC	9 cut(s)	183, 385, 388, 411, 416, 729, 1065, 1132, 1328
BstNI	CCWGG	1 cut(s)	783
BstNSI	RCATGY	1 cut(s)	988
BstPI	GGTNACC	1 cut(s)	1235
BstSCI	CCNGG	1 cut(s)	781
BstSFI	CTRYAG	1 cut(s)	216
BstSLI	GKGCMC	2 cut(s)	797, 1033
BstUI	CGCG	1 cut(s)	123
BstV1I	GCAGC	8 cut(s)	270, 391, 394, 397, 400, 1159, 1306, 1495
BstV2I	GAAGAC	1 cut(s)	819
BstX2I	RGATCY	2 cut(s)	76, 637
BstYI	RGATCY	2 cut(s)	76, 637
BstZI	CGGCCG	1 cut(s)	403
Bsu36I	CCTNAGG	1 cut(s)	521
BsuI	GTATCC	1 cut(s)	742
BsuRI	GGCC	6 cut(s)	161, 197, 296, 405, 601, 795
BtgZI	GCGATG	1 cut(s)	138
BtsCI	GGATG	6 cut(s)	319, 550, 571, 1159, 1204, 1398
BtsI	GCAGTG	1 cut(s)	1315
BtsIMutI	CAGTG	2 cut(s)	1293, 1315
Cac8I	GCNNGC	3 cut(s)	252, 412, 986
CaiI	CAGNNNCTG	2 cut(s)	1295, 1331
CciI	TCATGA	1 cut(s)	256
CfoI	GCGC	2 cut(s)	108, 123
Cfr13I	GGNCC	6 cut(s)	41, 159, 294, 793, 794, 1000
Csp6I	GTAC	2 cut(s)	614, 756
CviAII	CATG	7 cut(s)	257, 626, 734, 804, 974, 985, 1120
CviQI	GTAC	2 cut(s)	614, 756
DdeI	CTNAG	5 cut(s)	28, 287, 521, 540, 724
DpnI	GATC	6 cut(s)	78, 639, 729, 855, 978, 1283
DpnII	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
EaeI	YGGCCR	1 cut(s)	403
EagI	CGGCCG	1 cut(s)	403
Eam1104I	CTCTTC	3 cut(s)	8, 233, 324
EarI	CTCTTC	3 cut(s)	8, 233, 324
EciI	GGCGGA	1 cut(s)	201
Ecl136II	GAGCTC	1 cut(s)	410
EclXI	CGGCCG	1 cut(s)	403
Eco130I	CCWWGG	2 cut(s)	36, 297
Eco24I	GRGCYC	2 cut(s)	412, 797
Eco47I	GGWCC	2 cut(s)	41, 1000
Eco52I	CGGCCG	1 cut(s)	403
Eco53kI	GAGCTC	1 cut(s)	410
Eco57I	CTGAAG	2 cut(s)	813, 1367
Eco72I	CACGTG	1 cut(s)	673
Eco81I	CCTNAGG	1 cut(s)	521
Eco88I	CYCGRG	2 cut(s)	276, 392
Eco91I	GGTNACC	1 cut(s)	1235
EcoICRI	GAGCTC	1 cut(s)	410
EcoNI	CCTNNNNNAGG	1 cut(s)	1482
EcoO109I	RGGNCCY	4 cut(s)	41, 793, 794, 1000
EcoO65I	GGTNACC	1 cut(s)	1235
EcoRI	GAATTC	2 cut(s)	1076, 1420
EcoRII	CCWGG	1 cut(s)	781
EcoT14I	CCWWGG	2 cut(s)	36, 297
EcoT38I	GRGCYC	2 cut(s)	412, 797
ErhI	CCWWGG	2 cut(s)	36, 297
FaeI	CATG	7 cut(s)	260, 629, 737, 807, 977, 988, 1123
FaqI	GGGAC	3 cut(s)	54, 986, 1509
FatI	CATG	7 cut(s)	256, 625, 733, 803, 973, 984, 1119
FauNDI	CATATG	1 cut(s)	850
FokI	GGATG	6 cut(s)	326, 557, 578, 1146, 1211, 1385
FriOI	GRGCYC	2 cut(s)	412, 797
FspBI	CTAG	4 cut(s)	429, 603, 683, 1484
GlaI	GCGC	2 cut(s)	107, 122
GsaI	CCCAGC	1 cut(s)	1508
HaeII	RGCGCY	1 cut(s)	109
HaeIII	GGCC	6 cut(s)	161, 197, 296, 405, 601, 795
HapII	CCGG	2 cut(s)	109, 1086
HhaI	GCGC	2 cut(s)	108, 123
Hin1II	CATG	7 cut(s)	260, 629, 737, 807, 977, 988, 1123
Hin6I	GCGC	2 cut(s)	106, 121
HinP1I	GCGC	2 cut(s)	106, 121
HindIII	AAGCTT	2 cut(s)	1252, 1541
HinfI	GANTC	7 cut(s)	95, 140, 356, 446, 1010, 1209, 1521
HpaII	CCGG	2 cut(s)	109, 1086
HphI	GGTGA	2 cut(s)	338, 1214
Hpy166II	GTNNAC	2 cut(s)	668, 758
Hpy188III	TCNNGA	5 cut(s)	68, 257, 278, 1326, 1443
Hpy8I	GTNNAC	2 cut(s)	668, 758
Hpy99I	CGWCG	4 cut(s)	74, 149, 152, 167
HpyAV	CCTTC	5 cut(s)	91, 334, 338, 1465, 1482
HpyCH4III	ACNGT	5 cut(s)	490, 862, 967, 1297, 1449
HpyCH4IV	ACGT	2 cut(s)	672, 1015
HpyCH4V	TGCA	8 cut(s)	379, 988, 1037, 1143, 1175, 1322, 1436, 1460
HpyF10VI	GCNNNNNNNGC	9 cut(s)	183, 385, 388, 411, 416, 729, 1065, 1132, 1328
HpyF3I	CTNAG	5 cut(s)	28, 287, 521, 540, 724
HpySE526I	ACGT	2 cut(s)	672, 1015
Hsp92II	CATG	7 cut(s)	260, 629, 737, 807, 977, 988, 1123
HspAI	GCGC	2 cut(s)	106, 121
KflI	GGGWCCC	1 cut(s)	41
Kzo9I	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
LmnI	GCTCC	3 cut(s)	37, 280, 1328
Lsp1109I	GCAGC	8 cut(s)	270, 391, 394, 397, 400, 1159, 1306, 1495
LweI	GCATC	3 cut(s)	487, 1046, 1331
MaeI	CTAG	4 cut(s)	429, 603, 683, 1484
MaeII	ACGT	2 cut(s)	672, 1015
MaeIII	GTNAC	8 cut(s)	490, 785, 1011, 1220, 1235, 1291, 1310, 1389
MalI	GATC	6 cut(s)	78, 639, 729, 855, 978, 1283
MboI	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
MflI	RGATCY	2 cut(s)	76, 637
MhlI	GDGCHC	3 cut(s)	412, 797, 1033
MluCI	AATT	4 cut(s)	458, 1076, 1273, 1420
MlyI	GAGTC	2 cut(s)	350, 1019
MmeI	TCCRAC	4 cut(s)	150, 168, 949, 1390
MseI	TTAA	9 cut(s)	435, 618, 809, 864, 929, 962, 996, 1149, 1539
MslI	CAYNNNNRTG	2 cut(s)	564, 1048
MspA1I	CMGCKG	2 cut(s)	402, 1508
MspCI	CTTAAG	1 cut(s)	617
MspI	CCGG	2 cut(s)	109, 1086
MspR9I	CCNGG	1 cut(s)	783
Mva1269I	GAATGC	1 cut(s)	1159
MvaI	CCWGG	1 cut(s)	783
MvnI	CGCG	1 cut(s)	123
MwoI	GCNNNNNNNGC	9 cut(s)	183, 385, 388, 411, 416, 729, 1065, 1132, 1328
NdeI	CATATG	1 cut(s)	850
NdeII	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
NlaIII	CATG	7 cut(s)	260, 629, 737, 807, 977, 988, 1123
NmeAIII	GCCGAG	2 cut(s)	223, 431
NmuCI	GTSAC	5 cut(s)	785, 1011, 1220, 1291, 1310
NspI	RCATGY	1 cut(s)	988
PaeI	GCATGC	1 cut(s)	988
PaeR7I	CTCGAG	1 cut(s)	392
PagI	TCATGA	1 cut(s)	256
PctI	GAATGC	1 cut(s)	1159
PfeI	GAWTC	5 cut(s)	95, 140, 446, 1209, 1521
PflMI	CCANNNNNTGG	2 cut(s)	1278, 1409
PleI	GAGTC	2 cut(s)	350, 1018
PmaCI	CACGTG	1 cut(s)	673
PmlI	CACGTG	1 cut(s)	673
PpsI	GAGTC	2 cut(s)	350, 1018
Ppu21I	YACGTR	1 cut(s)	673
PpuMI	RGGWCCY	2 cut(s)	41, 1000
Psp124BI	GAGCTC	1 cut(s)	412
Psp5II	RGGWCCY	2 cut(s)	41, 1000
Psp6I	CCWGG	1 cut(s)	781
PspCI	CACGTG	1 cut(s)	673
PspEI	GGTNACC	1 cut(s)	1235
PspFI	CCCAGC	1 cut(s)	1504
PspGI	CCWGG	1 cut(s)	781
PspOMI	GGGCCC	1 cut(s)	793
PspPI	GGNCC	6 cut(s)	41, 159, 294, 793, 794, 1000
PspPPI	RGGWCCY	2 cut(s)	41, 1000
PspXI	VCTCGAGB	1 cut(s)	392
PstNI	CAGNNNCTG	2 cut(s)	1295, 1331
PsuI	RGATCY	2 cut(s)	76, 637
PvuII	CAGCTG	1 cut(s)	1508
RsaI	GTAC	2 cut(s)	615, 757
RsaNI	GTAC	2 cut(s)	614, 756
RseI	CAYNNNNRTG	2 cut(s)	564, 1048
SacI	GAGCTC	1 cut(s)	412
SaqAI	TTAA	9 cut(s)	435, 618, 809, 864, 929, 962, 996, 1149, 1539
Sau3AI	GATC	6 cut(s)	76, 637, 727, 853, 976, 1281
Sau96I	GGNCC	6 cut(s)	41, 159, 294, 793, 794, 1000
ScaI	AGTACT	1 cut(s)	615
SchI	GAGTC	2 cut(s)	350, 1019
ScrFI	CCNGG	1 cut(s)	783
SduI	GDGCHC	3 cut(s)	412, 797, 1033
SfaNI	GCATC	3 cut(s)	487, 1046, 1331
SfcI	CTRYAG	1 cut(s)	216
Sfr274I	CTCGAG	1 cut(s)	392
SinI	GGWCC	2 cut(s)	41, 1000
SlaI	CTCGAG	1 cut(s)	392
SmiMI	CAYNNNNRTG	2 cut(s)	564, 1048
SmlI	CTYRAG	2 cut(s)	392, 617
SmoI	CTYRAG	2 cut(s)	392, 617
SphI	GCATGC	1 cut(s)	988
Sse9I	AATT	4 cut(s)	458, 1076, 1273, 1420
SsiI	CCGC	7 cut(s)	21, 24, 183, 186, 324, 402, 1126
SspMI	CTAG	4 cut(s)	429, 603, 683, 1484
SstI	GAGCTC	1 cut(s)	412
StyD4I	CCNGG	1 cut(s)	781
StyI	CCWWGG	2 cut(s)	36, 297
TaaI	ACNGT	5 cut(s)	490, 862, 967, 1297, 1449
TaiI	ACGT	2 cut(s)	675, 1018
TaqI	TCGA	9 cut(s)	69, 117, 393, 481, 649, 856, 979, 1188, 1348
TasI	AATT	4 cut(s)	458, 1076, 1273, 1420
TatI	WGTACW	1 cut(s)	613
TauI	GCSGC	4 cut(s)	24, 27, 186, 405
TfiI	GAWTC	5 cut(s)	95, 140, 446, 1209, 1521
Tru1I	TTAA	9 cut(s)	435, 618, 809, 864, 929, 962, 996, 1149, 1539
Tru9I	TTAA	9 cut(s)	435, 618, 809, 864, 929, 962, 996, 1149, 1539
TscAI	CASTG	2 cut(s)	1300, 1315
TseFI	GTSAC	5 cut(s)	785, 1011, 1220, 1291, 1310
TseI	GCWGC	8 cut(s)	283, 379, 382, 385, 388, 1172, 1319, 1508
Tsp45I	GTSAC	5 cut(s)	785, 1011, 1220, 1291, 1310
TspDTI	ATGAA	9 cut(s)	17, 471, 624, 728, 792, 837, 1032, 1254, 1536
TspGWI	ACGGA	3 cut(s)	961, 1016, 1533
TspRI	CASTG	2 cut(s)	1300, 1315
Van91I	CCANNNNNTGG	2 cut(s)	1278, 1409
Vha464I	CTTAAG	1 cut(s)	617
VpaK11BI	GGWCC	2 cut(s)	41, 1000
XagI	CCTNNNNNAGG	1 cut(s)	1482
XapI	RAATTY	3 cut(s)	458, 1076, 1420
XceI	RCATGY	1 cut(s)	988
XhoI	CTCGAG	1 cut(s)	392
XspI	CTAG	4 cut(s)	429, 603, 683, 1484
ZrmI	AGTACT	1 cut(s)	615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.