Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

26919484 .. 26921347

Gene Structure 1864 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g25570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 873 bp

ATGAAGAACAAGAAGAGAGGCGGCAGCTCAGCTCCCAAGGGACCTAAAAAGGGCAAGGCTTTCTCTCTCGACGATGACCCCTTCTTCGGCGCCGAATCCAAGAAGCGCCGGAAAGTCGAGCTCGATGAGATTGAGAGCGAATCCGACGCCGACGTTGGGTCGGACGGCGAGGGCGGCGGGGAGGAGGCGGAGGAGGAGGAGGAGGAGGCTGTAGTGGAAACGGCAGACGAGAAGAGGAATAGGCTTGCGCATGAGTTTTTGGAGAACACTCGGGAACTGCTGAGGAGGGCGAAGGAAGATGAGGATGATGATAGTGGTGAAGAAGATGTGAAGGAAGGCGAGATGGACTCGCTTGTTGGCAAGATGTTGCAGCAGCAGCAGCTCGAGGACAGCGGCCGAGCTCGCCGTGTCATTGCTTCTAGGGTTAAAAAGCCAGAACCTACTGATGAATTTCGGATATTAGTGAAGCATCGACAACCTGTTACTGCTGTGGCTCTGTCTGAGGTTGACCTCAGGGGCTTTTCAGCTTCTAAGGATGGCACCATTCTACATTGGGATGTAGACAGTGGGAAAAGTGAAAAGTATCTATGGCCTAGTGATGCAGTATTAAAGTCCCATGGGGTCAAGGATCCACAAGGTCGAGCTAAAAAGCATAGTAAACATGTATTAGCACTAGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGGGGATTCGATCGCCATGTTCATTTGTGGGATACTCGTACACGAGAGCATATTCTGGCTTTTCCAGGTCACAGGGGACCTGTTTCATGTTTAACTTTCAGGCAAGGGTCTTCAGAACTGTTTTCTGGTTCATATGATCGAACAGTCATAAGATATGGAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

291

Amino Acids

32.36

Weight (kDa)

5.68

Isoelectric Point (pI)

56.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	151 - 278	1.5e-12	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	214 - 285	6.3e-12	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	215 - 286	5.7e-14	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	216 - 285	1.6e-13	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	216 - 277	1.2e-06	WDHD1 first WD40 domain
WD40	PF00400	217 - 247	1.2e-07	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	217 - 286	2.4e-06	WDR3 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	249
AccB1I	GGYRCC	2 cut(s)	89, 539
AccI	GTMKAC	1 cut(s)	561
AciI	CCGC	5 cut(s)	21, 174, 177, 188, 393
AclWI	GGATC	2 cut(s)	623, 636
AcoI	YGGCCR	1 cut(s)	394
AcsI	RAATTY	1 cut(s)	449
AcuI	CTGAAG	1 cut(s)	804
AcyI	GRCGYC	2 cut(s)	90, 147
AfaI	GTAC	1 cut(s)	748
AfiI	CCNNNNNNNGG	3 cut(s)	50, 86, 156
AflIII	ACRYGT	1 cut(s)	661
AhdI	GACNNNNNGTC	1 cut(s)	157
AjnI	CCWGG	1 cut(s)	772
AluBI	AGCT	7 cut(s)	27, 32, 121, 382, 401, 527, 644
AluI	AGCT	7 cut(s)	27, 32, 121, 382, 401, 527, 644
Alw21I	GWGCWC	2 cut(s)	123, 403
AlwI	GGATC	2 cut(s)	623, 636
Ama87I	CYCGRG	2 cut(s)	270, 383
AoxI	GGCC	2 cut(s)	394, 590
ApeKI	GCWGC	5 cut(s)	24, 370, 373, 376, 379
ApoI	RAATTY	1 cut(s)	449
ArsI	GACNNNNNNTTYG	2 cut(s)	68, 100
AspLEI	GCGC	3 cut(s)	92, 108, 250
AspS9I	GGNCC	2 cut(s)	41, 785
AsuHPI	GGTGA	1 cut(s)	329
AvaI	CYCGRG	2 cut(s)	270, 383
AvaII	GGWCC	2 cut(s)	41, 785
AxyI	CCTNAGG	1 cut(s)	512
BamHI	GGATCC	1 cut(s)	628
BanI	GGYRCC	2 cut(s)	89, 539
BanII	GRGCYC	2 cut(s)	123, 403
BauI	CACGAG	1 cut(s)	750
BbsI	GAAGAC	1 cut(s)	810
Bbv12I	GWGCWC	2 cut(s)	123, 403
BbvCI	CCTCAGC	1 cut(s)	281
BbvI	GCAGC	5 cut(s)	36, 382, 385, 388, 391
BccI	CCATC	3 cut(s)	337, 530, 683
BceAI	ACGGC	3 cut(s)	181, 237, 390
BciT130I	CCWGG	1 cut(s)	774
BciVI	GTATCC	1 cut(s)	733
BfaI	CTAG	3 cut(s)	420, 594, 674
BfmI	CTRYAG	1 cut(s)	210
BfoI	RGCGCY	2 cut(s)	93, 109
BfuI	GTATCC	1 cut(s)	733
BisI	GCNGC	8 cut(s)	22, 25, 175, 371, 374, 377, 380, 394
BlpI	GCTNAGC	1 cut(s)	28
BlsI	GCNGC	8 cut(s)	23, 26, 176, 372, 375, 378, 381, 395
Bme1390I	CCNGG	1 cut(s)	774
Bme18I	GGWCC	2 cut(s)	41, 785
BmeRI	GACNNNNNGTC	1 cut(s)	157
BmeT110I	CYCGRG	2 cut(s)	270, 383
BmgT120I	GGNCC	2 cut(s)	41, 785
BmiI	GGNNCC	5 cut(s)	42, 91, 541, 630, 786
BmrFI	CCNGG	1 cut(s)	774
BmsI	GCATC	2 cut(s)	478, 589
BpiI	GAAGAC	1 cut(s)	810
BplI	GAGNNNNNCTC	2 cut(s)	332, 364
Bpu10I	CCTNAGC	1 cut(s)	281
Bpu1102I	GCTNAGC	1 cut(s)	28
BsaHI	GRCGYC	2 cut(s)	90, 147
BsaJI	CCNNGG	2 cut(s)	36, 616
Bsc4I	CCNNNNNNNGG	3 cut(s)	50, 86, 156
Bse21I	CCTNAGG	1 cut(s)	512
Bse3DI	GCAATG	1 cut(s)	411
BseBI	CCWGG	1 cut(s)	774
BseDI	CCNNGG	2 cut(s)	36, 616
BseGI	GGATG	3 cut(s)	310, 541, 562
BseLI	CCNNNNNNNGG	3 cut(s)	50, 86, 156
BseMI	GCAATG	1 cut(s)	411
BseMII	CTCAG	4 cut(s)	42, 272, 492, 526
BseRI	GAGGAG	7 cut(s)	197, 206, 209, 212, 215, 218, 298
BseX3I	CGGCCG	1 cut(s)	394
BseXI	GCAGC	5 cut(s)	36, 382, 385, 388, 391
Bsh1285I	CGRYCG	2 cut(s)	397, 721
BshFI	GGCC	2 cut(s)	396, 592
BshNI	GGYRCC	2 cut(s)	89, 539
BsiEI	CGRYCG	2 cut(s)	397, 721
BsiHKAI	GWGCWC	2 cut(s)	123, 403
BsiHKCI	CYCGRG	2 cut(s)	270, 383
BsiSI	CCGG	1 cut(s)	109
BslFI	GGGAC	3 cut(s)	54, 598, 798
BslI	CCNNNNNNNGG	3 cut(s)	50, 86, 156
BsmFI	GGGAC	3 cut(s)	54, 598, 798
BsnI	GGCC	2 cut(s)	396, 592
BsoBI	CYCGRG	2 cut(s)	270, 383
Bsp1286I	GDGCHC	2 cut(s)	123, 403
Bsp143I	GATC	3 cut(s)	628, 718, 844
Bsp1720I	GCTNAGC	1 cut(s)	28
Bsp19I	CCATGG	1 cut(s)	616
BspACI	CCGC	5 cut(s)	21, 174, 177, 188, 393
BspANI	GGCC	2 cut(s)	396, 592
BspCNI	CTCAG	4 cut(s)	41, 273, 493, 525
BspLI	GGNNCC	5 cut(s)	42, 91, 541, 630, 786
BspPI	GGATC	2 cut(s)	623, 636
BspT107I	GGYRCC	2 cut(s)	89, 539
BsrDI	GCAATG	1 cut(s)	411
BssECI	CCNNGG	2 cut(s)	36, 616
BssMI	GATC	3 cut(s)	628, 718, 844
BssNI	GRCGYC	2 cut(s)	90, 147
BssSI	CACGAG	1 cut(s)	750
BssT1I	CCWWGG	2 cut(s)	36, 616
Bst2BI	CACGAG	1 cut(s)	750
Bst2UI	CCWGG	1 cut(s)	774
Bst4CI	ACNGT	3 cut(s)	566, 828, 853
Bst6I	CTCTTC	2 cut(s)	8, 227
BstACI	GRCGYC	2 cut(s)	90, 147
BstC8I	GCNNGC	2 cut(s)	246, 403
BstDEI	CTNAG	5 cut(s)	28, 281, 501, 512, 531
BstDSI	CCRYGG	1 cut(s)	616
BstF5I	GGATG	3 cut(s)	310, 541, 562
BstH2I	RGCGCY	2 cut(s)	93, 109
BstHHI	GCGC	3 cut(s)	92, 108, 250
BstKTI	GATC	3 cut(s)	631, 721, 847
BstMBI	GATC	3 cut(s)	628, 718, 844
BstMCI	CGRYCG	2 cut(s)	397, 721
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 376, 379, 402
BstNI	CCWGG	1 cut(s)	774
BstNSI	RCATGY	1 cut(s)	665
BstSCI	CCNGG	1 cut(s)	772
BstSFI	CTRYAG	1 cut(s)	210
BstV1I	GCAGC	5 cut(s)	36, 382, 385, 388, 391
BstV2I	GAAGAC	1 cut(s)	810
BstX2I	RGATCY	1 cut(s)	628
BstYI	RGATCY	1 cut(s)	628
BstZI	CGGCCG	1 cut(s)	394
Bsu36I	CCTNAGG	1 cut(s)	512
BsuI	GTATCC	1 cut(s)	733
BsuRI	GGCC	2 cut(s)	396, 592
BtgI	CCRYGG	1 cut(s)	616
BtsCI	GGATG	3 cut(s)	310, 541, 562
BtsIMutI	CAGTG	1 cut(s)	571
Cac8I	GCNNGC	2 cut(s)	246, 403
CfoI	GCGC	3 cut(s)	92, 108, 250
Cfr13I	GGNCC	2 cut(s)	41, 785
CseI	GACGC	1 cut(s)	155
Csp6I	GTAC	1 cut(s)	747
CviAII	CATG	5 cut(s)	251, 617, 662, 725, 795
CviQI	GTAC	1 cut(s)	747
DdeI	CTNAG	5 cut(s)	28, 281, 501, 512, 531
DinI	GGCGCC	1 cut(s)	91
DpnI	GATC	3 cut(s)	630, 720, 846
DpnII	GATC	3 cut(s)	628, 718, 844
DriI	GACNNNNNGTC	1 cut(s)	157
EaeI	YGGCCR	1 cut(s)	394
EagI	CGGCCG	1 cut(s)	394
Eam1104I	CTCTTC	2 cut(s)	8, 227
Eam1105I	GACNNNNNGTC	1 cut(s)	157
EarI	CTCTTC	2 cut(s)	8, 227
EciI	GGCGGA	1 cut(s)	203
Ecl136II	GAGCTC	2 cut(s)	121, 401
EclXI	CGGCCG	1 cut(s)	394
Eco130I	CCWWGG	2 cut(s)	36, 616
Eco24I	GRGCYC	2 cut(s)	123, 403
Eco47I	GGWCC	2 cut(s)	41, 785
Eco52I	CGGCCG	1 cut(s)	394
Eco53kI	GAGCTC	2 cut(s)	121, 401
Eco57I	CTGAAG	1 cut(s)	804
Eco81I	CCTNAGG	1 cut(s)	512
Eco88I	CYCGRG	2 cut(s)	270, 383
EcoICRI	GAGCTC	2 cut(s)	121, 401
EcoO109I	RGGNCCY	2 cut(s)	41, 785
EcoRII	CCWGG	1 cut(s)	772
EcoT14I	CCWWGG	2 cut(s)	36, 616
EcoT38I	GRGCYC	2 cut(s)	123, 403
EgeI	GGCGCC	1 cut(s)	91
EheI	GGCGCC	1 cut(s)	91
ErhI	CCWWGG	2 cut(s)	36, 616
FaeI	CATG	5 cut(s)	254, 620, 665, 728, 798
FaqI	GGGAC	3 cut(s)	54, 598, 798
FatI	CATG	5 cut(s)	250, 616, 661, 724, 794
FauI	CCCGC	1 cut(s)	170
FauNDI	CATATG	1 cut(s)	841
FblI	GTMKAC	1 cut(s)	561
Fnu4HI	GCNGC	8 cut(s)	22, 25, 175, 371, 374, 377, 380, 394
FokI	GGATG	3 cut(s)	317, 548, 569
FriOI	GRGCYC	2 cut(s)	123, 403
Fsp4HI	GCNGC	8 cut(s)	22, 25, 175, 371, 374, 377, 380, 394
FspBI	CTAG	3 cut(s)	420, 594, 674
FspI	TGCGCA	1 cut(s)	249
GlaI	GCGC	3 cut(s)	91, 107, 249
GluI	GCNGC	8 cut(s)	22, 25, 175, 371, 374, 377, 380, 394
HaeII	RGCGCY	2 cut(s)	93, 109
HaeIII	GGCC	2 cut(s)	396, 592
HapII	CCGG	1 cut(s)	109
HgaI	GACGC	1 cut(s)	155
HhaI	GCGC	3 cut(s)	92, 108, 250
Hin1I	GRCGYC	2 cut(s)	90, 147
Hin1II	CATG	5 cut(s)	254, 620, 665, 728, 798
Hin6I	GCGC	3 cut(s)	90, 106, 248
HinP1I	GCGC	3 cut(s)	90, 106, 248
HincII	GTYRAC	1 cut(s)	508
HindII	GTYRAC	1 cut(s)	508
HinfI	GANTC	4 cut(s)	95, 140, 347, 714
HpaII	CCGG	1 cut(s)	109
HphI	GGTGA	1 cut(s)	329
Hpy166II	GTNNAC	4 cut(s)	508, 562, 659, 749
Hpy188I	TCNGA	6 cut(s)	145, 163, 456, 502, 688, 823
Hpy188III	TCNNGA	2 cut(s)	68, 272
Hpy8I	GTNNAC	4 cut(s)	508, 562, 659, 749
Hpy99I	CGWCG	3 cut(s)	74, 149, 155
HpyAV	CCTTC	4 cut(s)	91, 286, 325, 329
HpyCH4III	ACNGT	3 cut(s)	566, 828, 853
HpyCH4IV	ACGT	1 cut(s)	153
HpyCH4V	TGCA	2 cut(s)	370, 602
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 376, 379, 402
HpyF3I	CTNAG	5 cut(s)	28, 281, 501, 512, 531
HpySE526I	ACGT	1 cut(s)	153
Hsp92I	GRCGYC	2 cut(s)	90, 147
Hsp92II	CATG	5 cut(s)	254, 620, 665, 728, 798
HspAI	GCGC	3 cut(s)	90, 106, 248
KasI	GGCGCC	1 cut(s)	89
Kzo9I	GATC	3 cut(s)	628, 718, 844
LmnI	GCTCC	1 cut(s)	37
Lsp1109I	GCAGC	5 cut(s)	36, 382, 385, 388, 391
LweI	GCATC	2 cut(s)	478, 589
MaeI	CTAG	3 cut(s)	420, 594, 674
MaeII	ACGT	1 cut(s)	153
MaeIII	GTNAC	2 cut(s)	481, 776
MalI	GATC	3 cut(s)	630, 720, 846
MboI	GATC	3 cut(s)	628, 718, 844
MboII	GAAGA	8 cut(s)	16, 25, 76, 244, 308, 332, 335, 810
MflI	RGATCY	1 cut(s)	628
MhlI	GDGCHC	2 cut(s)	123, 403
MluCI	AATT	1 cut(s)	449
Mly113I	GGCGCC	1 cut(s)	90
MlyI	GAGTC	1 cut(s)	341
MmeI	TCCRAC	2 cut(s)	141, 168
MseI	TTAA	3 cut(s)	426, 608, 800
MslI	CAYNNNNRTG	1 cut(s)	555
MspA1I	CMGCKG	1 cut(s)	393
MspI	CCGG	1 cut(s)	109
MspR9I	CCNGG	1 cut(s)	774
MvaI	CCWGG	1 cut(s)	774
MwoI	GCNNNNNNNGC	4 cut(s)	174, 376, 379, 402
NarI	GGCGCC	1 cut(s)	90
NcoI	CCATGG	1 cut(s)	616
NdeI	CATATG	1 cut(s)	841
NdeII	GATC	3 cut(s)	628, 718, 844
NlaIII	CATG	5 cut(s)	254, 620, 665, 728, 798
NlaIV	GGNNCC	5 cut(s)	42, 91, 541, 630, 786
NmeAIII	GCCGAG	1 cut(s)	422
NmuCI	GTSAC	1 cut(s)	776
NsbI	TGCGCA	1 cut(s)	249
NspI	RCATGY	1 cut(s)	665
PaeR7I	CTCGAG	1 cut(s)	383
PciI	ACATGT	1 cut(s)	661
PfeI	GAWTC	3 cut(s)	95, 140, 714
PkrI	GCNGC	8 cut(s)	23, 26, 176, 372, 375, 378, 381, 395
Ple19I	CGATCG	1 cut(s)	721
PleI	GAGTC	1 cut(s)	341
PluTI	GGCGCC	1 cut(s)	93
PpsI	GAGTC	1 cut(s)	341
PpuMI	RGGWCCY	2 cut(s)	41, 785
PscI	ACATGT	1 cut(s)	661
Psp124BI	GAGCTC	2 cut(s)	123, 403
Psp5II	RGGWCCY	2 cut(s)	41, 785
Psp6I	CCWGG	1 cut(s)	772
PspGI	CCWGG	1 cut(s)	772
PspN4I	GGNNCC	5 cut(s)	42, 91, 541, 630, 786
PspPI	GGNCC	2 cut(s)	41, 785
PspPPI	RGGWCCY	2 cut(s)	41, 785
PspXI	VCTCGAGB	1 cut(s)	383
PsuI	RGATCY	1 cut(s)	628
PvuI	CGATCG	1 cut(s)	721
RsaI	GTAC	1 cut(s)	748
RsaNI	GTAC	1 cut(s)	747
RseI	CAYNNNNRTG	1 cut(s)	555
SacI	GAGCTC	2 cut(s)	123, 403
SaqAI	TTAA	3 cut(s)	426, 608, 800
SatI	GCNGC	8 cut(s)	22, 25, 175, 371, 374, 377, 380, 394
Sau3AI	GATC	3 cut(s)	628, 718, 844
Sau96I	GGNCC	2 cut(s)	41, 785
SchI	GAGTC	1 cut(s)	341
ScrFI	CCNGG	1 cut(s)	774
SduI	GDGCHC	2 cut(s)	123, 403
SfaNI	GCATC	2 cut(s)	478, 589
SfcI	CTRYAG	1 cut(s)	210
SfoI	GGCGCC	1 cut(s)	91
Sfr274I	CTCGAG	1 cut(s)	383
SinI	GGWCC	2 cut(s)	41, 785
SlaI	CTCGAG	1 cut(s)	383
SmiMI	CAYNNNNRTG	1 cut(s)	555
SmlI	CTYRAG	1 cut(s)	383
SmoI	CTYRAG	1 cut(s)	383
Sse9I	AATT	1 cut(s)	449
SsiI	CCGC	5 cut(s)	21, 174, 177, 188, 393
SspDI	GGCGCC	1 cut(s)	89
SspMI	CTAG	3 cut(s)	420, 594, 674
SstI	GAGCTC	2 cut(s)	123, 403
StyD4I	CCNGG	1 cut(s)	772
StyI	CCWWGG	2 cut(s)	36, 616
TaaI	ACNGT	3 cut(s)	566, 828, 853
TaiI	ACGT	1 cut(s)	156
TaqI	TCGA	8 cut(s)	69, 117, 123, 384, 472, 640, 717, 847
TasI	AATT	1 cut(s)	449
TauI	GCSGC	3 cut(s)	24, 177, 396
TfiI	GAWTC	3 cut(s)	95, 140, 714
Tru1I	TTAA	3 cut(s)	426, 608, 800
Tru9I	TTAA	3 cut(s)	426, 608, 800
TscAI	CASTG	1 cut(s)	571
TseFI	GTSAC	1 cut(s)	776
TseI	GCWGC	5 cut(s)	24, 370, 373, 376, 379
Tsp45I	GTSAC	1 cut(s)	776
TspDTI	ATGAA	5 cut(s)	17, 462, 719, 783, 828
TspRI	CASTG	1 cut(s)	571
VpaK11BI	GGWCC	2 cut(s)	41, 785
XapI	RAATTY	1 cut(s)	449
XceI	RCATGY	1 cut(s)	665
XhoI	CTCGAG	1 cut(s)	383
XmiI	GTMKAC	1 cut(s)	561
XspI	CTAG	3 cut(s)	420, 594, 674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.