Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0012065

Physical Location & Seq

Genomic Coordinates Forward (+)

11678802 .. 11682332

Gene Structure 3531 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G010640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGAACAGGAACACGAAGCGAGGCGGCGGCCGCGGTGGGGGAGGCTCAGCTCCCAAGGGCGGAAGAGGAGGAGACAGAAAGGGAGCCAAAAAAGGCAAGAGGCAGTCGTTCAAGGAAGACCCATTTTTCACCAACGAATCCAAGAAGCGGCTCAAATACGACGAGGACATTCGGAGCGACGAGTCGTCCGACGATGAACGAAACGACGCCGTCGGTAGTGGCGAGGAGGAGGAGGAGGAGGAGGCGGCTGAGGAGATTATAGAGGAGACTCCCGGGGAGAAGAAGCTGAGGCTGACGAAGGAGTATCTGAAGAGGCTTGGAGAGCATGAGAGGAAGAAGAAGGAAGACGAGGATGACGATAGCGATGACGACAGCGAGAAGGAAGGCGACAGAGACTCGCTTGTCGCCAAGGTTTTGCAGCAGCAGCAGCTCCAGGACAGCGGCCGCCTCCGCCGCTCCATTGCTTCTAGGGTTCAGAAGCCAGAAGCTACTGATGAGTTTCGGATCTTAGTGAAGCATCGCCAATCTGTCACTGCAGTGGTTCTCTCTGATGATGATGGCAGAGGTTTTTCAGCATCTAAGGACGGCGCGATTTTGCATTGGGATGTAGATAGTGGGAAGAAAGAGAAGTATCCGTGGCCCAGTGACGAAGTATTGAAGTCCCATGGGGTCAAGGAACCACTAGGTAGAGCTACAAAGCATAGCAAACAAATGTTAGCACTTGCAGTTAGTTCTGATGGTCGGTATTTGGCAAGTGGAGGATTAGATCGCCATATTCATTTGTGGGATACTCGTACCAGACAGCATGTTCAGGCTTTTCCGGGTCACAGGGGACCTGTATCATGTTTAACTTTCAGGCAAGGGAGTTCAGAACTCTTTTCTGGTTCATATGATCGAACAGTTAAGATATGGAATGCAGAAGACAGAACTTACATAAATACATTATATGGTCACCAAGGTGAAGTATTATCTATGGATTGCCTACGGAAAGAACGGCTTTTGACTGTTGGACATGATCGAAGCATGATGTTATTCAAGGTTCCAGAGGAGTCGCGTTTAGTATTCCGTGCCCCTGCCTGCTCATTGGAGTGTTGTTCTTTTATTAGCAATGATGAATTCTTATCCGGCTCTGATAATGGAAGTGTTGAGCTTTGGACCATGCTGCGAAAGAAGCCTGTATGCATAGTTAAGAATGCTCATCCTTTGTTAGCTGCACACAAAGATGTTGAACAGAAGGATGGTGATAGAGTCCCCAATGGTCACATGGAAAGTTATGATTGTTCATCAACATATTCTTGGATCAGTTCAGTCACTTCTTGTCGAGGCAGTGACCTTGCCGCATCAGGAGCTGGTAATGGCTTTGTTCGTTTATGGTCTATTGAAAGTGAGAGCAATGCCATTCAGCCCTTACACAGTCTCCCCGTGGTTGGATTTGTTAATTCTTTAGCTATTGCAAAATCTGGAGGCTTTCTAGTTGCAGGAGTCGGAAAGGAACCGCGTCTAGGAAGGTGGGGACTTATCCCAGCTGCTCAGAATGGAGTTGCAATCCACACCCTTAAGCTTTCGTCAGATACAAATAAGCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007154 GO:0007275 GO:0008037 GO:0008150 GO:0008152 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009875 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016072 GO:0022414 GO:0022613 GO:0030515 GO:0030684 GO:0031428 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044706 GO:0046483 GO:0048229 GO:0048316 GO:0048544 GO:0048608 GO:0048731 GO:0048856 GO:0051704 GO:0060321 GO:0061458 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

528

Amino Acids

58.49

Weight (kDa)

6.62

Isoelectric Point (pI)

49.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	164 - 348	8.1e-17	CDC20/Fizzy WD40 domain
WDR55	PF24796	164 - 396	1.9e-13	WDR55
Beta-prop_CAF1B_HIR1	PF24105	170 - 314	9.6e-08	CAF1B/HIR1 beta-propeller domain
NBCH_WD40	PF20426	205 - 329	2.5e-06	Neurobeachin beta propeller domain
Beta-prop_TEP1_2nd	PF25047	213 - 343	1.6e-12	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	226 - 287	1.4e-10	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	229 - 346	2.6e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	231 - 263	2.7e-06	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	232 - 392	4.2e-23	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	232 - 400	5.6e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	233 - 347	2.3e-23	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	234 - 387	1.2e-10	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_1st	PF24780	235 - 310	8.1e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	235 - 338	1.6e-06	WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	235 - 350	7e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	236 - 350	3.1e-15	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	236 - 343	1.7e-06	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	236 - 394	4.2e-19	Prp19 WD40 domain
WD40	PF00400	269 - 305	2.5e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	276 - 351	2.5e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G05410 AT4G21130
fragaria_vesca	FvH4_3g09990
malus_domestica	MD05G1274300.v1.1 MD10G1254000.v1.1
prunus_persica	Prupe.4G089600_v2.0.a1
pyrus_communis	pycom05g25570 pycom05g25580 pycom10g20960 pycom10g20970
rosa_chinensis	RchiOBHm_Chr5g0015871
rosa_laevigata	RLG00000032227
rosa_roxburghii	Rroxscaffold_1G00060730
rosa_rugosa	Rorug05G0029700
rosa_samantha	Rh5AG123300 Rh5BG122200 Rh5CG133400 Rh5DG121600
rosa_wichuraiana	Rw5G010640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	401
AccBSI	CCGCTC	1 cut(s)	456
AccII	CGCG	4 cut(s)	33, 590, 1054, 1498
AclWI	GGATC	2 cut(s)	512, 1307
AcoI	YGGCCR	2 cut(s)	28, 442
AcsI	RAATTY	1 cut(s)	1115
AcuI	CTGAAG	1 cut(s)	329
AcyI	GRCGYC	1 cut(s)	207
AfaI	GTAC	1 cut(s)	796
AfiI	CCNNNNNNNGG	5 cut(s)	37, 59, 147, 1427, 1502
AflII	CTTAAG	1 cut(s)	1556
AgsI	TTSAA	5 cut(s)	112, 658, 1036, 1229, 1382
AhdI	GACNNNNNGTC	1 cut(s)	184
AjnI	CCWGG	1 cut(s)	432
AleI	CACNNNNGTG	2 cut(s)	536, 957
Alw26I	GTCTC	4 cut(s)	66, 260, 387, 1421
AlwI	GGATC	2 cut(s)	512, 1307
AlwNI	CAGNNNCTG	1 cut(s)	1349
Ama87I	CYCGRG	1 cut(s)	272
AoxI	GGCC	3 cut(s)	28, 442, 638
ApeKI	GCWGC	7 cut(s)	418, 421, 424, 427, 1162, 1211, 1526
ApoI	RAATTY	1 cut(s)	1115
AspLEI	GCGC	1 cut(s)	590
AspS9I	GGNCC	3 cut(s)	639, 833, 1155
AsuC2I	CCSGG	3 cut(s)	273, 274, 822
AsuHPI	GGTGA	4 cut(s)	121, 944, 971, 1253
AvaI	CYCGRG	1 cut(s)	272
AvaII	GGWCC	2 cut(s)	833, 1155
BaeGI	GKGCMC	1 cut(s)	1072
BbsI	GAAGAC	3 cut(s)	123, 351, 927
BbvCI	CCTCAGC	2 cut(s)	249, 287
BbvI	GCAGC	7 cut(s)	430, 433, 436, 439, 1149, 1198, 1513
BccI	CCATC	3 cut(s)	551, 731, 1232
BceAI	ACGGC	3 cut(s)	194, 601, 1010
BciT130I	CCWGG	1 cut(s)	434
BciVI	GTATCC	2 cut(s)	642, 781
BcnI	CCSGG	3 cut(s)	273, 274, 822
BcoDI	GTCTC	4 cut(s)	66, 260, 387, 1421
BfaI	CTAG	4 cut(s)	468, 683, 1472, 1502
BfmI	CTRYAG	1 cut(s)	534
BfrI	CTTAAG	1 cut(s)	1556
BfuI	GTATCC	2 cut(s)	642, 781
BlpI	GCTNAGC	1 cut(s)	46
Bme1390I	CCNGG	4 cut(s)	273, 274, 434, 822
Bme18I	GGWCC	2 cut(s)	833, 1155
BmeRI	GACNNNNNGTC	1 cut(s)	184
BmeT110I	CYCGRG	1 cut(s)	272
BmgT120I	GGNCC	3 cut(s)	639, 833, 1155
BmiI	GGNNCC	5 cut(s)	85, 678, 834, 1041, 1494
BmrFI	CCNGG	4 cut(s)	273, 274, 434, 822
BmrI	ACTGGG	1 cut(s)	636
BmsI	GCATC	3 cut(s)	526, 584, 1349
BmuI	ACTGGG	1 cut(s)	636
BpiI	GAAGAC	3 cut(s)	123, 351, 927
BpmI	CTGGAG	2 cut(s)	416, 1482
Bpu10I	CCTNAGC	2 cut(s)	249, 287
Bpu1102I	GCTNAGC	1 cut(s)	46
BpuMI	CCSGG	3 cut(s)	273, 274, 822
BsaHI	GRCGYC	1 cut(s)	207
BsaJI	CCNNGG	9 cut(s)	31, 54, 272, 273, 408, 635, 664, 955, 1422
BsaXI	ACNNNNNCTCC	2 cut(s)	1401, 1431
Bsc4I	CCNNNNNNNGG	5 cut(s)	37, 59, 147, 1427, 1502
Bse1I	ACTGG	1 cut(s)	642
Bse3DI	GCAATG	3 cut(s)	459, 1114, 1399
BseBI	CCWGG	1 cut(s)	434
BseDI	CCNNGG	9 cut(s)	31, 54, 272, 273, 408, 635, 664, 955, 1422
BseGI	GGATG	4 cut(s)	358, 610, 1198, 1243
BseLI	CCNNNNNNNGG	5 cut(s)	37, 59, 147, 1427, 1502
BseMI	GCAATG	3 cut(s)	459, 1114, 1399
BseMII	CTCAG	4 cut(s)	60, 240, 278, 1544
BseNI	ACTGG	1 cut(s)	642
BseSI	GKGCMC	1 cut(s)	1072
BseX3I	CGGCCG	2 cut(s)	28, 442
BseXI	GCAGC	7 cut(s)	430, 433, 436, 439, 1149, 1198, 1513
BseYI	CCCAGC	1 cut(s)	1522
BsgI	GTGCAG	1 cut(s)	1197
Bsh1236I	CGCG	4 cut(s)	33, 590, 1054, 1498
Bsh1285I	CGRYCG	2 cut(s)	31, 445
BshFI	GGCC	3 cut(s)	30, 444, 640
BsiEI	CGRYCG	2 cut(s)	31, 445
BsiHKCI	CYCGRG	1 cut(s)	272
BsiSI	CCGG	3 cut(s)	273, 821, 1125
BslFI	GGGAC	4 cut(s)	646, 846, 1235, 1527
BslI	CCNNNNNNNGG	5 cut(s)	37, 59, 147, 1427, 1502
BsmAI	GTCTC	4 cut(s)	66, 260, 387, 1421
BsmFI	GGGAC	4 cut(s)	646, 846, 1235, 1527
BsmI	GAATGC	2 cut(s)	919, 1198
BsnI	GGCC	3 cut(s)	30, 444, 640
BsoBI	CYCGRG	1 cut(s)	272
Bsp1286I	GDGCHC	1 cut(s)	1072
Bsp143I	GATC	5 cut(s)	504, 766, 892, 1015, 1299
Bsp1720I	GCTNAGC	1 cut(s)	46
Bsp19I	CCATGG	1 cut(s)	664
BspANI	GGCC	3 cut(s)	30, 444, 640
BspCNI	CTCAG	4 cut(s)	59, 241, 279, 1543
BspFNI	CGCG	4 cut(s)	33, 590, 1054, 1498
BspLI	GGNNCC	5 cut(s)	85, 678, 834, 1041, 1494
BspMAI	CTGCAG	1 cut(s)	538
BspPI	GGATC	2 cut(s)	512, 1307
BspTI	CTTAAG	1 cut(s)	1556
BsrBI	CCGCTC	1 cut(s)	456
BsrDI	GCAATG	3 cut(s)	459, 1114, 1399
BsrI	ACTGG	1 cut(s)	642
BssECI	CCNNGG	9 cut(s)	31, 54, 272, 273, 408, 635, 664, 955, 1422
BssMI	GATC	5 cut(s)	504, 766, 892, 1015, 1299
BssNI	GRCGYC	1 cut(s)	207
BssT1I	CCWWGG	4 cut(s)	54, 408, 664, 955
Bst2UI	CCWGG	1 cut(s)	434
Bst4CI	ACNGT	3 cut(s)	901, 1006, 1415
Bst6I	CTCTTC	2 cut(s)	58, 305
BstACI	GRCGYC	1 cut(s)	207
BstAFI	CTTAAG	1 cut(s)	1556
BstC8I	GCNNGC	1 cut(s)	1078
BstDEI	CTNAG	6 cut(s)	46, 249, 287, 508, 579, 1530
BstDSI	CCRYGG	4 cut(s)	31, 635, 664, 1422
BstEII	GGTNACC	1 cut(s)	950
BstF5I	GGATG	4 cut(s)	358, 610, 1198, 1243
BstFNI	CGCG	4 cut(s)	33, 590, 1054, 1498
BstHHI	GCGC	1 cut(s)	590
BstKTI	GATC	5 cut(s)	507, 769, 895, 1018, 1302
BstMAI	GTCTC	4 cut(s)	66, 260, 387, 1421
BstMBI	GATC	5 cut(s)	504, 766, 892, 1015, 1299
BstMCI	CGRYCG	2 cut(s)	31, 445
BstMWI	GCNNNNNNNGC	8 cut(s)	30, 322, 424, 427, 450, 453, 1171, 1346
BstNI	CCWGG	1 cut(s)	434
BstNSI	RCATGY	1 cut(s)	809
BstPI	GGTNACC	1 cut(s)	950
BstSCI	CCNGG	4 cut(s)	271, 272, 432, 820
BstSFI	CTRYAG	1 cut(s)	534
BstSLI	GKGCMC	1 cut(s)	1072
BstUI	CGCG	4 cut(s)	33, 590, 1054, 1498
BstV1I	GCAGC	7 cut(s)	430, 433, 436, 439, 1149, 1198, 1513
BstV2I	GAAGAC	3 cut(s)	123, 351, 927
BstX2I	RGATCY	1 cut(s)	504
BstYI	RGATCY	1 cut(s)	504
BstZI	CGGCCG	2 cut(s)	28, 442
BsuI	GTATCC	2 cut(s)	642, 781
BsuRI	GGCC	3 cut(s)	30, 444, 640
BtgI	CCRYGG	4 cut(s)	31, 635, 664, 1422
BtgZI	GCGATG	2 cut(s)	378, 503
BtsCI	GGATG	4 cut(s)	358, 610, 1198, 1243
BtsI	GCAGTG	3 cut(s)	531, 543, 1333
BtsIMutI	CAGTG	4 cut(s)	531, 543, 649, 1333
Cac8I	GCNNGC	1 cut(s)	1078
CaiI	CAGNNNCTG	1 cut(s)	1349
CciNI	GCGGCCGC	2 cut(s)	28, 442
CfoI	GCGC	1 cut(s)	590
Cfr13I	GGNCC	3 cut(s)	639, 833, 1155
Cfr42I	CCGCGG	1 cut(s)	34
Cfr9I	CCCGGG	1 cut(s)	272
CseI	GACGC	2 cut(s)	215, 1487
Csp6I	GTAC	1 cut(s)	795
CviAII	CATG	8 cut(s)	326, 665, 806, 843, 1013, 1024, 1159, 1264
CviQI	GTAC	1 cut(s)	795
DdeI	CTNAG	6 cut(s)	46, 249, 287, 508, 579, 1530
DpnI	GATC	5 cut(s)	506, 768, 894, 1017, 1301
DpnII	GATC	5 cut(s)	504, 766, 892, 1015, 1299
DrdI	GACNNNNNNGTC	1 cut(s)	401
DriI	GACNNNNNGTC	1 cut(s)	184
DseDI	GACNNNNNNGTC	1 cut(s)	401
EaeI	YGGCCR	2 cut(s)	28, 442
EagI	CGGCCG	2 cut(s)	28, 442
Eam1104I	CTCTTC	2 cut(s)	58, 305
Eam1105I	GACNNNNNGTC	1 cut(s)	184
EarI	CTCTTC	2 cut(s)	58, 305
EciI	GGCGGA	2 cut(s)	75, 440
EclXI	CGGCCG	2 cut(s)	28, 442
Eco130I	CCWWGG	4 cut(s)	54, 408, 664, 955
Eco47I	GGWCC	2 cut(s)	833, 1155
Eco52I	CGGCCG	2 cut(s)	28, 442
Eco57I	CTGAAG	1 cut(s)	329
Eco88I	CYCGRG	1 cut(s)	272
Eco91I	GGTNACC	1 cut(s)	950
EcoO109I	RGGNCCY	1 cut(s)	833
EcoO65I	GGTNACC	1 cut(s)	950
EcoRI	GAATTC	1 cut(s)	1115
EcoRII	CCWGG	1 cut(s)	432
EcoT14I	CCWWGG	4 cut(s)	54, 408, 664, 955
EcoT22I	ATGCAT	1 cut(s)	1184
ErhI	CCWWGG	4 cut(s)	54, 408, 664, 955
FaeI	CATG	8 cut(s)	329, 668, 809, 846, 1016, 1027, 1162, 1267
FalI	AAGNNNNNCTT	2 cut(s)	981, 1013
FaqI	GGGAC	4 cut(s)	646, 846, 1235, 1527
FatI	CATG	8 cut(s)	325, 664, 805, 842, 1012, 1023, 1158, 1263
FauNDI	CATATG	1 cut(s)	889
FokI	GGATG	4 cut(s)	365, 617, 1185, 1250
FspBI	CTAG	4 cut(s)	468, 683, 1472, 1502
GlaI	GCGC	1 cut(s)	589
GsaI	CCCAGC	1 cut(s)	1526
GsuI	CTGGAG	2 cut(s)	416, 1482
HaeIII	GGCC	3 cut(s)	30, 444, 640
HapII	CCGG	3 cut(s)	273, 821, 1125
HgaI	GACGC	2 cut(s)	215, 1487
HhaI	GCGC	1 cut(s)	590
Hin1I	GRCGYC	1 cut(s)	207
Hin1II	CATG	8 cut(s)	329, 668, 809, 846, 1016, 1027, 1162, 1267
Hin6I	GCGC	1 cut(s)	588
HinP1I	GCGC	1 cut(s)	588
HindIII	AAGCTT	2 cut(s)	1559, 1580
HinfI	GANTC	7 cut(s)	137, 182, 268, 395, 1049, 1248, 1482
HpaII	CCGG	3 cut(s)	273, 821, 1125
HphI	GGTGA	4 cut(s)	121, 944, 971, 1253
Hpy188III	TCNNGA	3 cut(s)	1043, 1344, 1461
Hpy99I	CGWCG	5 cut(s)	164, 182, 194, 209, 215
HpyAV	CCTTC	6 cut(s)	292, 334, 373, 377, 1228, 1500
HpyCH4III	ACNGT	3 cut(s)	901, 1006, 1415
HpyF10VI	GCNNNNNNNGC	8 cut(s)	30, 322, 424, 427, 450, 453, 1171, 1346
HpyF3I	CTNAG	6 cut(s)	46, 249, 287, 508, 579, 1530
Hsp92I	GRCGYC	1 cut(s)	207
Hsp92II	CATG	8 cut(s)	329, 668, 809, 846, 1016, 1027, 1162, 1267
HspAI	GCGC	1 cut(s)	588
KspI	CCGCGG	1 cut(s)	34
Kzo9I	GATC	5 cut(s)	504, 766, 892, 1015, 1299
LmnI	GCTCC	6 cut(s)	55, 83, 174, 435, 461, 1346
Lsp1109I	GCAGC	7 cut(s)	430, 433, 436, 439, 1149, 1198, 1513
LweI	GCATC	3 cut(s)	526, 584, 1349
MaeI	CTAG	4 cut(s)	468, 683, 1472, 1502
MaeIII	GTNAC	7 cut(s)	529, 644, 824, 950, 1259, 1309, 1328
MalI	GATC	5 cut(s)	506, 768, 894, 1017, 1301
MbiI	CCGCTC	1 cut(s)	456
MboI	GATC	5 cut(s)	504, 766, 892, 1015, 1299
MboII	GAAGA	9 cut(s)	75, 128, 292, 322, 346, 349, 356, 631, 932
MflI	RGATCY	1 cut(s)	504
MhlI	GDGCHC	1 cut(s)	1072
MluCI	AATT	2 cut(s)	1115, 1438
MlyI	GAGTC	6 cut(s)	191, 262, 389, 1058, 1257, 1491
MmeI	TCCRAC	4 cut(s)	213, 988, 1408, 1465
Mph1103I	ATGCAT	1 cut(s)	1184
MseI	TTAA	5 cut(s)	848, 903, 1188, 1437, 1557
MslI	CAYNNNNRTG	5 cut(s)	536, 603, 957, 1087, 1221
MspA1I	CMGCKG	3 cut(s)	33, 441, 1526
MspCI	CTTAAG	1 cut(s)	1556
MspI	CCGG	3 cut(s)	273, 821, 1125
MspR9I	CCNGG	4 cut(s)	273, 274, 434, 822
Mva1269I	GAATGC	2 cut(s)	919, 1198
MvaI	CCWGG	1 cut(s)	434
MvnI	CGCG	4 cut(s)	33, 590, 1054, 1498
MwoI	GCNNNNNNNGC	8 cut(s)	30, 322, 424, 427, 450, 453, 1171, 1346
NciI	CCSGG	3 cut(s)	273, 274, 822
NcoI	CCATGG	1 cut(s)	664
NdeI	CATATG	1 cut(s)	889
NdeII	GATC	5 cut(s)	504, 766, 892, 1015, 1299
NlaIII	CATG	8 cut(s)	329, 668, 809, 846, 1016, 1027, 1162, 1267
NlaIV	GGNNCC	5 cut(s)	85, 678, 834, 1041, 1494
NmuCI	GTSAC	7 cut(s)	529, 644, 824, 950, 1259, 1309, 1328
NotI	GCGGCCGC	2 cut(s)	28, 442
NsiI	ATGCAT	1 cut(s)	1184
NspI	RCATGY	1 cut(s)	809
OliI	CACNNNNGTG	2 cut(s)	536, 957
PcsI	WCGNNNNNNNCGW	3 cut(s)	186, 219, 354
PctI	GAATGC	2 cut(s)	919, 1198
PfeI	GAWTC	1 cut(s)	137
PflFI	GACNNNGTC	1 cut(s)	209
PfoI	TCCNGGA	1 cut(s)	432
PleI	GAGTC	6 cut(s)	190, 262, 389, 1057, 1256, 1490
PpsI	GAGTC	6 cut(s)	190, 262, 389, 1057, 1256, 1490
PpuMI	RGGWCCY	1 cut(s)	833
Psp5II	RGGWCCY	1 cut(s)	833
Psp6I	CCWGG	1 cut(s)	432
PspEI	GGTNACC	1 cut(s)	950
PspFI	CCCAGC	1 cut(s)	1522
PspGI	CCWGG	1 cut(s)	432
PspN4I	GGNNCC	5 cut(s)	85, 678, 834, 1041, 1494
PspPI	GGNCC	3 cut(s)	639, 833, 1155
PspPPI	RGGWCCY	1 cut(s)	833
PstI	CTGCAG	1 cut(s)	538
PstNI	CAGNNNCTG	1 cut(s)	1349
PsuI	RGATCY	1 cut(s)	504
PsyI	GACNNNGTC	1 cut(s)	209
PvuII	CAGCTG	1 cut(s)	1526
RsaI	GTAC	1 cut(s)	796
RsaNI	GTAC	1 cut(s)	795
RseI	CAYNNNNRTG	5 cut(s)	536, 603, 957, 1087, 1221
SacII	CCGCGG	1 cut(s)	34
SaqAI	TTAA	5 cut(s)	848, 903, 1188, 1437, 1557
Sau3AI	GATC	5 cut(s)	504, 766, 892, 1015, 1299
Sau96I	GGNCC	3 cut(s)	639, 833, 1155
SchI	GAGTC	6 cut(s)	191, 262, 389, 1058, 1257, 1491
ScrFI	CCNGG	4 cut(s)	273, 274, 434, 822
SduI	GDGCHC	1 cut(s)	1072
SfaNI	GCATC	3 cut(s)	526, 584, 1349
SfcI	CTRYAG	1 cut(s)	534
Sfr303I	CCGCGG	1 cut(s)	34
SgrBI	CCGCGG	1 cut(s)	34
SinI	GGWCC	2 cut(s)	833, 1155
SmaI	CCCGGG	1 cut(s)	274
SmiMI	CAYNNNNRTG	5 cut(s)	536, 603, 957, 1087, 1221
SmlI	CTYRAG	1 cut(s)	1556
SmoI	CTYRAG	1 cut(s)	1556
Sse9I	AATT	2 cut(s)	1115, 1438
SspMI	CTAG	4 cut(s)	468, 683, 1472, 1502
StyD4I	CCNGG	4 cut(s)	271, 272, 432, 820
StyI	CCWWGG	4 cut(s)	54, 408, 664, 955
TaaI	ACNGT	3 cut(s)	901, 1006, 1415
TaqI	TCGA	3 cut(s)	895, 1018, 1321
TasI	AATT	2 cut(s)	1115, 1438
TauI	GCSGC	9 cut(s)	27, 30, 33, 151, 248, 444, 447, 456, 1340
TfiI	GAWTC	1 cut(s)	137
Tru1I	TTAA	5 cut(s)	848, 903, 1188, 1437, 1557
Tru9I	TTAA	5 cut(s)	848, 903, 1188, 1437, 1557
TscAI	CASTG	4 cut(s)	538, 543, 649, 1333
TseFI	GTSAC	7 cut(s)	529, 644, 824, 950, 1259, 1309, 1328
TseI	GCWGC	7 cut(s)	418, 421, 424, 427, 1162, 1211, 1526
Tsp45I	GTSAC	7 cut(s)	529, 644, 824, 950, 1259, 1309, 1328
TspDTI	ATGAA	6 cut(s)	17, 210, 767, 876, 1128, 1272
TspGWI	ACGGA	3 cut(s)	624, 1000, 1055
TspMI	CCCGGG	1 cut(s)	272
TspRI	CASTG	4 cut(s)	538, 543, 649, 1333
Tth111I	GACNNNGTC	1 cut(s)	209
Vha464I	CTTAAG	1 cut(s)	1556
VpaK11BI	GGWCC	2 cut(s)	833, 1155
XapI	RAATTY	1 cut(s)	1115
XceI	RCATGY	1 cut(s)	809
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1261
XmaI	CCCGGG	1 cut(s)	272
XspI	CTAG	4 cut(s)	468, 683, 1472, 1502
Zsp2I	ATGCAT	1 cut(s)	1184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.