FvH4_3g12150

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010189
Physical Location & Seq
Forward (+)
7202208 .. 7204339
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12150.t1

Sequence Viewer

Length: 498 bp
ATGGAGAAGGGTATCCAAGGGACCATCAATCTTAGAAGATATGGAGAAGAAGAAGAAGAAGCTGTGGATTTGAGCGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAATTCAACGGCCCTTGTGACGTCTCCGACTACTTCAAACCCAAACCCACCGGGATGGAGTCTGATGGTTTGAAGACGCAGGAGGCTTATTTTCGAGGAAGGAAGCTGCAGGGTGCAAATGTTCCCATCCCAGATGGGTATTCCGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCCAAGATATCTGAAGGGAATTCGAACTGTTGGGAGATAAATGCCAAATATAAAAGCATTACATATTGGAATCATGACAGCGTTCCTTCGCAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAACAGCAGAAGATTTGGTTTCTGCATCAGCTGTTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

166

Amino Acids

18.42

Weight (kDa)

6.43

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 28 - 145 1.3e-29 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 96
AatII GACGTC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 75
AccIII TCCGGA 1 cut(s) 248
AciI CCGC 1 cut(s) 75
AcsI RAATTY 2 cut(s) 107, 310
AcuI CTGAAG 1 cut(s) 324
AcyI GRCGYC 1 cut(s) 126
AgsI TTSAA 4 cut(s) 112, 142, 178, 493
AluBI AGCT 4 cut(s) 62, 211, 290, 476
AluI AGCT 4 cut(s) 62, 211, 290, 476
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 133
Alw44I GTGCAC 1 cut(s) 83
Aor13HI TCCGGA 1 cut(s) 248
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 99, 211
ApoI RAATTY 2 cut(s) 107, 310
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 21, 116
AsuC2I CCSGG 1 cut(s) 157
AsuII TTCGAA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 86, 198
BccI CCATC 5 cut(s) 32, 154, 164, 233, 239
BceAI ACGGC 1 cut(s) 130
BciVI GTATCC 1 cut(s) 23
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 133
BfmI CTRYAG 2 cut(s) 212, 420
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 2 cut(s) 100, 212
BlsI GCNGC 2 cut(s) 101, 213
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 21, 116
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 157
BmsI GCATC 3 cut(s) 111, 379, 479
BpiI GAAGAC 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 16, 259
BsaWI WCCGGW 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 443
BseAI TCCGGA 1 cut(s) 248
BseDI CCNNGG 2 cut(s) 16, 259
BseGI GGATG 3 cut(s) 165, 231, 391
BseNI ACTGG 1 cut(s) 443
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 2 cut(s) 86, 198
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 2 cut(s) 156, 249
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 133
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 248
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 248
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 1 cut(s) 443
BssECI CCNNGG 2 cut(s) 16, 259
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 16, 259
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 3 cut(s) 165, 231, 391
BstMAI GTCTC 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 2 cut(s) 212, 420
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 86, 198
BstV2I GAAGAC 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 160
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 165, 231, 391
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 417
CciI TCATGA 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 21, 116
CseI GACGC 1 cut(s) 190
CviAII CATG 2 cut(s) 365, 437
CviJI RGCY 7 cut(s) 62, 117, 191, 211, 290, 415, 476
CviKI_1 RGCY 7 cut(s) 62, 117, 191, 211, 290, 415, 476
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 16, 259
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 310
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 2 cut(s) 16, 259
EcoT22I ATGCAT 2 cut(s) 394, 438
ErhI CCWWGG 2 cut(s) 16, 259
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 2 cut(s) 368, 440
FaiI YATR 6 cut(s) 42, 342, 355, 366, 434, 438
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 364, 436
Fnu4HI GCNGC 2 cut(s) 100, 212
FokI GGATG 3 cut(s) 172, 218, 398
Fsp4HI GCNGC 2 cut(s) 100, 212
GluI GCNGC 2 cut(s) 100, 212
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 156, 249
HgaI GACGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 2 cut(s) 368, 440
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 3 cut(s) 164, 271, 361
HpaII CCGG 2 cut(s) 156, 249
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 133, 169, 304
Hpy188III TCNNGA 4 cut(s) 249, 275, 365, 482
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 3 cut(s) 198, 299, 387
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 7 cut(s) 85, 102, 214, 221, 392, 436, 470
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 2 cut(s) 368, 440
Kpn2I TCCGGA 1 cut(s) 248
LmnI GCTCC 1 cut(s) 96
Lsp1109I GCAGC 2 cut(s) 86, 198
LweI GCATC 3 cut(s) 111, 379, 479
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 122, 445
MbiI CCGCTC 1 cut(s) 75
MboII GAAGA 9 cut(s) 48, 59, 62, 65, 68, 190, 277, 280, 467
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 2 cut(s) 107, 310
MlyI GAGTC 2 cut(s) 173, 280
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 2 cut(s) 181, 194
Mph1103I ATGCAT 2 cut(s) 394, 438
MroI TCCGGA 1 cut(s) 248
MroXI GAANNNNTTC 1 cut(s) 289
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 476
MspI CCGG 2 cut(s) 156, 249
MspR9I CCNGG 1 cut(s) 157
Mva1269I GAATGC 1 cut(s) 392
NciI CCSGG 1 cut(s) 157
NlaIII CATG 2 cut(s) 368, 440
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 2 cut(s) 394, 438
NspV TTCGAA 1 cut(s) 314
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 1 cut(s) 96
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 101, 213
PleI GAGTC 2 cut(s) 172, 279
PpsI GAGTC 2 cut(s) 172, 279
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 21, 116
PstI CTGCAG 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 158
SatI GCNGC 2 cut(s) 100, 212
Sau96I GGNCC 2 cut(s) 21, 116
SchI GAGTC 2 cut(s) 173, 280
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 1 cut(s) 87
SetI ASST 7 cut(s) 64, 84, 90, 129, 213, 292, 478
SfaNI GCATC 3 cut(s) 111, 379, 479
SfcI CTRYAG 2 cut(s) 212, 420
SfuI TTCGAA 1 cut(s) 314
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 2 cut(s) 107, 310
SsiI CCGC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 2 cut(s) 16, 259
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 199, 314
TasI AATT 2 cut(s) 107, 310
TfiI GAWTC 1 cut(s) 361
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 2 cut(s) 99, 211
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 398, 453
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 2 cut(s) 107, 310
XmnI GAANNNNTTC 1 cut(s) 289
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 2 cut(s) 394, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.