Rroxscaffold_1G00058280

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010189
Physical Location & Seq
Reverse (-)
80130274 .. 80133056
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058280.1

Sequence Viewer

Length: 546 bp
ATGGAGAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAGAAGAAGAAGAAACTGTGGATTTGAGTGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAATACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCTTAAAGACGCAGGAGGCTTGTTTCCGAGGACGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGTGTGTTTCTGATTGTTTCGATCATCTTTACTATGTATTGCCTCGCAGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCAAATATATCTGAAGGGAATTCAAACTTTTGGGAGATAAATGCCAAATATAAAAACATTACATATTGGAATCATGACAGCCTTCCTTCGCAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAGCAGCAGAAGATTTGGTTTCTGCATCCGCTGCTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

181

Amino Acids

20.11

Weight (kDa)

6.1

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 28 - 161 1.7e-27 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 96
AciI CCGC 1 cut(s) 522
AcsI RAATTY 1 cut(s) 358
AcuI CTGAAG 1 cut(s) 372
AgsI TTSAA 4 cut(s) 142, 342, 363, 541
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw21I GWGCWC 1 cut(s) 87
Alw44I GTGCAC 1 cut(s) 83
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 99, 497, 524
ApoI RAATTY 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 337
AspS9I GGNCC 2 cut(s) 21, 116
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 86, 509, 511
BccI CCATC 2 cut(s) 233, 239
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 468
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 3 cut(s) 100, 498, 525
BlsI GCNGC 3 cut(s) 101, 499, 526
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 21, 116
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 4 cut(s) 111, 230, 427, 527
BsaJI CCNNGG 4 cut(s) 16, 168, 199, 307
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse1I ACTGG 2 cut(s) 160, 491
BseDI CCNNGG 4 cut(s) 16, 168, 199, 307
BseGI GGATG 4 cut(s) 221, 231, 439, 518
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 160, 491
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 3 cut(s) 86, 509, 511
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 87
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 1 cut(s) 270
BspACI CCGC 1 cut(s) 522
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 96
BsrI ACTGG 2 cut(s) 160, 491
BssECI CCNNGG 4 cut(s) 16, 168, 199, 307
BssMI GATC 1 cut(s) 270
BssT1I CCWWGG 2 cut(s) 16, 307
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 465
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 221, 231, 439, 518
BstKTI GATC 1 cut(s) 273
BstMBI GATC 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 86, 509, 511
BstXI CCANNNNNNTGG 1 cut(s) 160
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 4 cut(s) 221, 231, 439, 518
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 465
CciI TCATGA 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 21, 116
CseI GACGC 1 cut(s) 190
CviAII CATG 2 cut(s) 413, 485
CviJI RGCY 6 cut(s) 117, 174, 191, 338, 420, 463
CviKI_1 RGCY 6 cut(s) 117, 174, 191, 338, 420, 463
DdeI CTNAG 1 cut(s) 32
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco130I CCWWGG 2 cut(s) 16, 307
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 372
EcoRI GAATTC 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 16, 307
EcoT22I ATGCAT 2 cut(s) 442, 486
ErhI CCWWGG 2 cut(s) 16, 307
FaeI CATG 2 cut(s) 416, 488
FaiI YATR 8 cut(s) 26, 284, 347, 390, 403, 414, 482, 486
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 412, 484
Fnu4HI GCNGC 3 cut(s) 100, 498, 525
FokI GGATG 4 cut(s) 208, 218, 446, 505
Fsp4HI GCNGC 3 cut(s) 100, 498, 525
GluI GCNGC 3 cut(s) 100, 498, 525
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 190
Hin1II CATG 2 cut(s) 416, 488
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 3 cut(s) 164, 319, 409
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 6 cut(s) 33, 133, 169, 200, 261, 352
Hpy188III TCNNGA 3 cut(s) 323, 413, 530
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 3 cut(s) 347, 431, 435
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 5 cut(s) 85, 102, 440, 484, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 416, 488
Kzo9I GATC 1 cut(s) 270
LmnI GCTCC 2 cut(s) 96, 218
Lsp1109I GCAGC 3 cut(s) 86, 509, 511
LweI GCATC 4 cut(s) 111, 230, 427, 527
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 122
MalI GATC 1 cut(s) 272
MboI GATC 1 cut(s) 270
MboII GAAGA 8 cut(s) 48, 59, 62, 65, 68, 325, 328, 515
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 1 cut(s) 358
MlyI GAGTC 2 cut(s) 173, 328
MnlI CCTC 4 cut(s) 163, 181, 194, 302
Mph1103I ATGCAT 2 cut(s) 442, 486
MroXI GAANNNNTTC 1 cut(s) 337
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 440
MwoI GCNNNNNNNGC 1 cut(s) 524
NdeII GATC 1 cut(s) 270
NlaIII CATG 2 cut(s) 416, 488
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 2 cut(s) 442, 486
PagI TCATGA 1 cut(s) 412
PaqCI CACCTGC 1 cut(s) 96
PctI GAATGC 1 cut(s) 440
PdmI GAANNNNTTC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 409
PkrI GCNGC 3 cut(s) 101, 499, 526
PleI GAGTC 2 cut(s) 172, 327
PpsI GAGTC 2 cut(s) 172, 327
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 21, 116
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 3 cut(s) 100, 498, 525
Sau3AI GATC 1 cut(s) 270
Sau96I GGNCC 2 cut(s) 21, 116
SchI GAGTC 2 cut(s) 173, 328
SduI GDGCHC 1 cut(s) 87
SetI ASST 4 cut(s) 84, 90, 129, 340
SfaNI GCATC 4 cut(s) 111, 230, 427, 527
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 1 cut(s) 358
SsiI CCGC 1 cut(s) 522
StyI CCWWGG 2 cut(s) 16, 307
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 269
TasI AATT 1 cut(s) 358
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 3 cut(s) 99, 497, 524
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 446, 501
TspGWI ACGGA 2 cut(s) 214, 220
TspRI CASTG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 337
Zsp2I ATGCAT 2 cut(s) 442, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.