MD10G1234600.v1.1

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010189
Physical Location & Seq
Reverse (-)
33068179 .. 33071169
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1234600.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGAGCAGGGCACCCAAGGCAGCATAGTTCTGAGAAGAAATGGAAACGGAGAGTCACAGGGAGAAGAAGAAAGAGTGGTGGACTTGAGTGGTCAGGTGCACCTCCTCCCCTGCTCCATCAAATTCAATGGCCCTTCCTCCGTCTCCCAATACTTCAAACCCAAATCAACTGGAATCGAATCAGAGGGTTTAAGAACACAGGAAGCTTATTTCAGAGGAAGGAAGTTGCAAGGAGCCTCGATTCCGATTCCAGATGGGTATTCCGGATTTGTTCTAGGGAAGAAGAGTCTTGGCAAGAGAAAAGCTTCCAATAATTCAGATGGGAGCTCAAACTGTTGGGAGATGAATGCGAAATACAAAAGCATCACATATTGGAATCACGACAGCCTTCCTTCACAGGATGATGCATTCTTGCGTTGTTTTCACTGGCTTTCTGTAGCAAAATCTATGCACGAACCAGCAACACCCGGAGATTTGGTTTCTGCATCAGCTGCATTGGAAAAGATGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

171

Amino Acids

18.63

Weight (kDa)

8.42

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 33 - 150 9.8e-28 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 263
AcsI RAATTY 1 cut(s) 122
AgsI TTSAA 2 cut(s) 127, 157
AluBI AGCT 4 cut(s) 206, 305, 327, 491
AluI AGCT 4 cut(s) 206, 305, 327, 491
Alw21I GWGCWC 2 cut(s) 102, 329
Alw26I GTCTC 1 cut(s) 148
Alw44I GTGCAC 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 263
AoxI GGCC 1 cut(s) 130
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 21, 491
ApoI RAATTY 1 cut(s) 122
Asp700I GAANNNNTTC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 468
BaeGI GKGCMC 2 cut(s) 14, 102
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 329
Bbv12I GWGCWC 2 cut(s) 102, 329
BbvI GCAGC 2 cut(s) 33, 478
BccI CCATC 3 cut(s) 125, 248, 314
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 1 cut(s) 148
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 2 cut(s) 22, 492
BlsI GCNGC 2 cut(s) 23, 493
Bme1390I CCNGG 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 13, 235
BmrFI CCNGG 1 cut(s) 468
BmsI GCATC 3 cut(s) 372, 394, 494
BpuEI CTTGAG 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 468
BsaJI CCNNGG 1 cut(s) 16
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse1I ACTGG 2 cut(s) 175, 431
BseAI TCCGGA 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 16
BseGI GGATG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 175, 431
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 2 cut(s) 14, 102
BseXI GCAGC 2 cut(s) 33, 478
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 1 cut(s) 11
BsiHKAI GWGCWC 2 cut(s) 102, 329
BsiSI CCGG 2 cut(s) 264, 468
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
BsmI GAATGC 2 cut(s) 352, 407
BsnI GGCC 1 cut(s) 132
Bsp1286I GDGCHC 3 cut(s) 14, 102, 329
Bsp13I TCCGGA 1 cut(s) 263
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 24
BspEI TCCGGA 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 13, 235
BspT107I GGYRCC 1 cut(s) 11
BsrI ACTGG 2 cut(s) 175, 431
BssECI CCNNGG 1 cut(s) 16
BssT1I CCWWGG 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 335
Bst6I CTCTTC 1 cut(s) 278
BstAPI GCANNNNNTGC 1 cut(s) 491
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 18, 491
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 435
BstSLI GKGCMC 2 cut(s) 14, 102
BstV1I GCAGC 2 cut(s) 33, 478
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 1 cut(s) 424
Cfr13I GGNCC 1 cut(s) 131
CviJI RGCY 8 cut(s) 132, 206, 236, 305, 327, 387, 430, 491
CviKI_1 RGCY 8 cut(s) 132, 206, 236, 305, 327, 387, 430, 491
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 16
EcoT22I ATGCAT 1 cut(s) 409
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 16
Esp3I CGTCTC 1 cut(s) 148
FaiI YATR 3 cut(s) 26, 370, 449
Fnu4HI GCNGC 2 cut(s) 22, 492
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 2 cut(s) 22, 492
FspBI CTAG 1 cut(s) 275
GluI GCNGC 2 cut(s) 22, 492
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 264, 468
HindIII AAGCTT 2 cut(s) 204, 303
HinfI GANTC 7 cut(s) 53, 174, 179, 241, 247, 286, 376
HpaII CCGG 2 cut(s) 264, 468
Hpy166II GTNNAC 2 cut(s) 82, 100
Hpy188I TCNGA 5 cut(s) 33, 184, 215, 246, 319
Hpy188III TCNNGA 3 cut(s) 251, 264, 380
Hpy8I GTNNAC 2 cut(s) 82, 100
HpyAV CCTTC 4 cut(s) 144, 213, 398, 402
HpyCH4III ACNGT 1 cut(s) 335
HpyCH4V TGCA 6 cut(s) 100, 229, 407, 451, 485, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 491
HpyF3I CTNAG 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 263
LmnI GCTCC 4 cut(s) 4, 119, 233, 324
Lsp1109I GCAGC 2 cut(s) 33, 478
LweI GCATC 3 cut(s) 372, 394, 494
MaeI CTAG 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 54
MboII GAAGA 5 cut(s) 48, 77, 80, 292, 295
MhlI GDGCHC 3 cut(s) 14, 102, 329
MluCI AATT 2 cut(s) 122, 313
MlyI GAGTC 2 cut(s) 62, 295
MnlI CCTC 6 cut(s) 113, 116, 148, 178, 209, 247
Mph1103I ATGCAT 1 cut(s) 409
MroI TCCGGA 1 cut(s) 263
MroXI GAANNNNTTC 1 cut(s) 304
MseI TTAA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 264, 468
MspR9I CCNGG 1 cut(s) 468
Mva1269I GAATGC 2 cut(s) 352, 407
MwoI GCNNNNNNNGC 2 cut(s) 18, 491
NciI CCSGG 1 cut(s) 468
NlaIV GGNNCC 2 cut(s) 13, 235
NmuCI GTSAC 1 cut(s) 54
NsiI ATGCAT 1 cut(s) 409
PctI GAATGC 2 cut(s) 352, 407
PdmI GAANNNNTTC 1 cut(s) 304
PfeI GAWTC 5 cut(s) 174, 179, 241, 247, 376
PkrI GCNGC 2 cut(s) 23, 493
PleI GAGTC 2 cut(s) 61, 294
PpsI GAGTC 2 cut(s) 61, 294
Psp124BI GAGCTC 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 13, 235
PspPI GGNCC 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 2 cut(s) 22, 492
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 2 cut(s) 62, 295
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 3 cut(s) 14, 102, 329
SetI ASST 6 cut(s) 99, 105, 208, 307, 329, 493
SfaNI GCATC 3 cut(s) 372, 394, 494
SfcI CTRYAG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 85
SmoI CTYRAG 1 cut(s) 85
Sse9I AATT 2 cut(s) 122, 313
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 1 cut(s) 466
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 177, 239
TasI AATT 2 cut(s) 122, 313
TfiI GAWTC 5 cut(s) 174, 179, 241, 247, 376
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TscAI CASTG 1 cut(s) 431
TseFI GTSAC 1 cut(s) 54
TseI GCWGC 2 cut(s) 21, 491
Tsp45I GTSAC 1 cut(s) 54
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 2 cut(s) 63, 130
TspRI CASTG 1 cut(s) 431
VneI GTGCAC 1 cut(s) 98
XapI RAATTY 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 304
XspI CTAG 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.