Rw5G012810

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010189
Physical Location & Seq
Forward (+)
15197596 .. 15200109
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012810.1

Sequence Viewer

Length: 486 bp
ATGGACAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAAGTGTGGATTTGAGTGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAATACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCTTAAAGACGCATGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCCAATATATCTGAAGGGAATTCAAACTGTTGGGAGATAAATGCCAAATATAAAAGCATTACATATTGGAACCATGACAGCCTTCCTTCACAGGATGATGCATTCTTGCGTTCTTTTCAGTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAACAGCAGAAGATTTGGTTTCTGCATCCGCTGCTCTGGAAAAGTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

161

Amino Acids

17.75

Weight (kDa)

8.24

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 24 - 141 1.1e-27 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 84
AciI CCGC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 312
AgsI TTSAA 3 cut(s) 130, 303, 481
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
Alw21I GWGCWC 1 cut(s) 75
Alw44I GTGCAC 1 cut(s) 71
AoxI GGCC 1 cut(s) 103
ApaLI GTGCAC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 87, 464
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 21, 104
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 75
BaeI ACNNNNGTAYC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 2 cut(s) 74, 451
BccI CCATC 2 cut(s) 221, 227
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 84
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 2 cut(s) 88, 465
BlsI GCNGC 2 cut(s) 89, 466
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 21, 104
BmiI GGNNCC 2 cut(s) 22, 350
BmsI GCATC 4 cut(s) 99, 218, 367, 467
BsaJI CCNNGG 4 cut(s) 16, 156, 187, 247
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 198, 228
Bse1I ACTGG 2 cut(s) 148, 431
BseDI CCNNGG 4 cut(s) 16, 156, 187, 247
BseGI GGATG 4 cut(s) 209, 219, 379, 458
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 148, 431
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 2 cut(s) 74, 451
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 75
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 75
BspACI CCGC 1 cut(s) 462
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 22, 350
BspMI ACCTGC 1 cut(s) 84
BsrI ACTGG 2 cut(s) 148, 431
BssECI CCNNGG 4 cut(s) 16, 156, 187, 247
BssT1I CCWWGG 2 cut(s) 16, 247
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 251
BstAPI GCANNNNNTGC 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 209, 219, 379, 458
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 74, 451
BstXI CCANNNNNNTGG 1 cut(s) 148
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 4 cut(s) 209, 219, 379, 458
BtsIMutI CAGTG 2 cut(s) 141, 404
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 405
Cfr13I GGNCC 2 cut(s) 21, 104
CseI GACGC 1 cut(s) 178
CviAII CATG 3 cut(s) 173, 353, 425
CviJI RGCY 6 cut(s) 105, 162, 179, 278, 360, 403
CviKI_1 RGCY 6 cut(s) 105, 162, 179, 278, 360, 403
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 16, 247
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 16, 247
EcoT22I ATGCAT 2 cut(s) 382, 426
ErhI CCWWGG 2 cut(s) 16, 247
FaeI CATG 3 cut(s) 176, 356, 428
FaiI YATR 8 cut(s) 26, 174, 287, 330, 343, 354, 422, 426
FaqI GGGAC 1 cut(s) 34
FatI CATG 3 cut(s) 172, 352, 424
Fnu4HI GCNGC 2 cut(s) 88, 465
FokI GGATG 4 cut(s) 196, 206, 386, 445
Fsp4HI GCNGC 2 cut(s) 88, 465
GluI GCNGC 2 cut(s) 88, 465
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 1 cut(s) 105
HgaI GACGC 1 cut(s) 178
Hin1II CATG 3 cut(s) 176, 356, 428
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 2 cut(s) 152, 259
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 5 cut(s) 33, 121, 157, 188, 292
Hpy188III TCNNGA 3 cut(s) 237, 263, 470
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 186, 287, 371, 375
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 5 cut(s) 73, 90, 380, 424, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 3 cut(s) 176, 356, 428
LmnI GCTCC 2 cut(s) 84, 206
LpnPI CCDG 9 cut(s) 89, 97, 129, 188, 222, 237, 356, 444, 455
Lsp1109I GCAGC 2 cut(s) 74, 451
LweI GCATC 4 cut(s) 99, 218, 367, 467
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 110, 433
MboII GAAGA 4 cut(s) 48, 265, 268, 455
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 298
MlyI GAGTC 2 cut(s) 161, 268
MnlI CCTC 3 cut(s) 151, 169, 182
Mph1103I ATGCAT 2 cut(s) 382, 426
MroXI GAANNNNTTC 1 cut(s) 277
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 146
MspA1I CMGCKG 1 cut(s) 464
Mva1269I GAATGC 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 464
NlaIII CATG 3 cut(s) 176, 356, 428
NlaIV GGNNCC 2 cut(s) 22, 350
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 2 cut(s) 382, 426
PaqCI CACCTGC 1 cut(s) 84
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 277
PkrI GCNGC 2 cut(s) 89, 466
PleI GAGTC 2 cut(s) 160, 267
PpsI GAGTC 2 cut(s) 160, 267
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 22, 350
PspPI GGNCC 2 cut(s) 21, 104
RseI CAYNNNNRTG 1 cut(s) 146
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 2 cut(s) 88, 465
Sau96I GGNCC 2 cut(s) 21, 104
SchI GAGTC 2 cut(s) 161, 268
SduI GDGCHC 1 cut(s) 75
SetI ASST 4 cut(s) 72, 78, 117, 280
SfaNI GCATC 4 cut(s) 99, 218, 367, 467
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 1 cut(s) 298
SsiI CCGC 1 cut(s) 462
StyI CCWWGG 2 cut(s) 16, 247
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 117
TasI AATT 1 cut(s) 298
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 2 cut(s) 148, 404
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 87, 464
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 441
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 2 cut(s) 148, 404
VneI GTGCAC 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 277
Zsp2I ATGCAT 2 cut(s) 382, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.