Rh5AG144900

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010189
Physical Location & Seq
Forward (+)
15304265 .. 15307138
2874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG144900.1

Sequence Viewer

Length: 483 bp
ATGGACAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAACTGTGGATTTGAGTGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAATACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCCTAAAGACGCAGGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCCAATATATCTGAAGGGAATTCAAACTGTTGGGAGATAAATGCCAAATATAAAAGCATTACATATTGGAACCATGACAGCCTTCCTTCACAGGATGATGCATTCTTGCGTTCTTTTCAGTGGCTTGCTGTAGCAAAAACTGTAAGTATGAACACCTCATATGTATCAACCAAAAAAGACAAAACCTCGTATGGTGTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

160

Amino Acids

17.76

Weight (kDa)

9.16

Isoelectric Point (pI)

27.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 24 - 140 6.1e-28 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 84
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 312
AgsI TTSAA 2 cut(s) 130, 303
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
Alw21I GWGCWC 1 cut(s) 75
Alw44I GTGCAC 1 cut(s) 71
AoxI GGCC 2 cut(s) 103, 160
ApaLI GTGCAC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 87
ApoI RAATTY 1 cut(s) 298
Asp700I GAANNNNTTC 1 cut(s) 277
AspS9I GGNCC 3 cut(s) 21, 104, 160
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 75
BaeI ACNNNNGTAYC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 74
BccI CCATC 2 cut(s) 221, 227
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 84
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 1 cut(s) 88
BlsI GCNGC 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 21, 104, 160
BmiI GGNNCC 2 cut(s) 22, 350
BmsI GCATC 3 cut(s) 99, 218, 367
BsaJI CCNNGG 4 cut(s) 16, 156, 187, 247
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 198, 228
Bse1I ACTGG 1 cut(s) 148
BseDI CCNNGG 4 cut(s) 16, 156, 187, 247
BseGI GGATG 3 cut(s) 209, 219, 379
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 1 cut(s) 74
BseYI CCCAGC 1 cut(s) 202
BshFI GGCC 2 cut(s) 105, 162
BsiHKAI GWGCWC 1 cut(s) 75
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 2 cut(s) 105, 162
Bsp1286I GDGCHC 1 cut(s) 75
BspANI GGCC 2 cut(s) 105, 162
BspCNI CTCAG 1 cut(s) 24
BspLI GGNNCC 2 cut(s) 22, 350
BspMI ACCTGC 1 cut(s) 84
BsrI ACTGG 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 16, 156, 187, 247
BssT1I CCWWGG 2 cut(s) 16, 247
Bst4CI ACNGT 3 cut(s) 52, 308, 421
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 3 cut(s) 209, 219, 379
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 148
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 105, 162
BtsCI GGATG 3 cut(s) 209, 219, 379
BtsIMutI CAGTG 2 cut(s) 141, 404
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 405
Cfr13I GGNCC 3 cut(s) 21, 104, 160
CseI GACGC 1 cut(s) 178
CviAII CATG 1 cut(s) 353
CviJI RGCY 6 cut(s) 105, 162, 179, 278, 360, 403
CviKI_1 RGCY 6 cut(s) 105, 162, 179, 278, 360, 403
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco130I CCWWGG 2 cut(s) 16, 247
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 16, 247
EcoT22I ATGCAT 1 cut(s) 382
ErhI CCWWGG 2 cut(s) 16, 247
FaeI CATG 1 cut(s) 356
FaiI YATR 9 cut(s) 26, 287, 330, 343, 354, 428, 439, 441, 471
FaqI GGGAC 1 cut(s) 34
FatI CATG 1 cut(s) 352
FauNDI CATATG 1 cut(s) 439
Fnu4HI GCNGC 1 cut(s) 88
FokI GGATG 3 cut(s) 196, 206, 386
Fsp4HI GCNGC 1 cut(s) 88
GluI GCNGC 1 cut(s) 88
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 2 cut(s) 105, 162
HgaI GACGC 1 cut(s) 178
Hin1II CATG 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 2 cut(s) 152, 259
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 5 cut(s) 33, 121, 157, 188, 292
Hpy188III TCNNGA 2 cut(s) 237, 263
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 186, 287, 371, 375
HpyCH4III ACNGT 3 cut(s) 52, 308, 421
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 73, 90, 380
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 1 cut(s) 356
LmnI GCTCC 2 cut(s) 84, 206
LpnPI CCDG 8 cut(s) 89, 97, 129, 158, 188, 222, 237, 356
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 3 cut(s) 99, 218, 367
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 110
MboII GAAGA 3 cut(s) 48, 265, 268
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 298
MlyI GAGTC 2 cut(s) 161, 268
MnlI CCTC 5 cut(s) 151, 169, 182, 445, 475
Mph1103I ATGCAT 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 277
MslI CAYNNNNRTG 1 cut(s) 146
Mva1269I GAATGC 1 cut(s) 380
NdeI CATATG 1 cut(s) 439
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 2 cut(s) 22, 350
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 84
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 277
PkrI GCNGC 1 cut(s) 89
PleI GAGTC 2 cut(s) 160, 267
PpsI GAGTC 2 cut(s) 160, 267
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 22, 350
PspPI GGNCC 3 cut(s) 21, 104, 160
RseI CAYNNNNRTG 1 cut(s) 146
SatI GCNGC 1 cut(s) 88
Sau96I GGNCC 3 cut(s) 21, 104, 160
SchI GAGTC 2 cut(s) 161, 268
SduI GDGCHC 1 cut(s) 75
SetI ASST 6 cut(s) 72, 78, 117, 280, 437, 467
SfaNI GCATC 3 cut(s) 99, 218, 367
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 146
Sse9I AATT 1 cut(s) 298
StyI CCWWGG 2 cut(s) 16, 247
TaaI ACNGT 3 cut(s) 52, 308, 421
TaiI ACGT 1 cut(s) 117
TasI AATT 1 cut(s) 298
TscAI CASTG 2 cut(s) 148, 404
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 1 cut(s) 87
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 1 cut(s) 443
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 2 cut(s) 148, 404
VneI GTGCAC 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 298
XmnI GAANNNNTTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.