Rh5BG144600

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010189
Physical Location & Seq
Forward (+)
14985087 .. 14987409
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG144600.1

Sequence Viewer

Length: 495 bp
ATGGAGAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAGAAGAAGAAACTGTGGATTTGAGTGGTAAGGTTCACCTGCTCCCCTGCTGCATCAAATACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCCTAAAGACGCAGGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCCAATATATCTGAAGGGAGTTCAAACTGTTGGGAGATAAATGCCAAATATAAAAGCATTACATATTGGAATCATGACAGCCTTCCTTCACAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAACAGCAGAAGATTTGGTTTCTGCATCCGCTGCTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

164

Amino Acids

18.14

Weight (kDa)

6.43

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 27 - 144 8.3e-28 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 93
AciI CCGC 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 321
AgsI TTSAA 3 cut(s) 139, 312, 490
AloI GAACNNNNNNTCC 2 cut(s) 207, 239
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
AoxI GGCC 2 cut(s) 112, 169
ApeKI GCWGC 2 cut(s) 96, 473
Asp700I GAANNNNTTC 1 cut(s) 286
AspS9I GGNCC 3 cut(s) 21, 113, 169
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 21
BbvI GCAGC 2 cut(s) 83, 460
BccI CCATC 2 cut(s) 230, 236
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 93
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 2 cut(s) 97, 474
BlsI GCNGC 2 cut(s) 98, 475
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 21, 113, 169
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 4 cut(s) 108, 227, 376, 476
BsaJI CCNNGG 4 cut(s) 16, 165, 196, 256
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse1I ACTGG 2 cut(s) 157, 440
BseDI CCNNGG 4 cut(s) 16, 165, 196, 256
BseGI GGATG 4 cut(s) 218, 228, 388, 467
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 157, 440
BseXI GCAGC 2 cut(s) 83, 460
BseYI CCCAGC 1 cut(s) 211
BshFI GGCC 2 cut(s) 114, 171
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 2 cut(s) 114, 171
BspACI CCGC 1 cut(s) 471
BspANI GGCC 2 cut(s) 114, 171
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 93
BsrI ACTGG 2 cut(s) 157, 440
BssECI CCNNGG 4 cut(s) 16, 165, 196, 256
BssT1I CCWWGG 2 cut(s) 16, 256
Bst4CI ACNGT 2 cut(s) 61, 317
Bst6I CTCTTC 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 218, 228, 388, 467
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 83, 460
BstXI CCANNNNNNTGG 1 cut(s) 157
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 114, 171
BtsCI GGATG 4 cut(s) 218, 228, 388, 467
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 414
CciI TCATGA 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 21, 113, 169
CseI GACGC 1 cut(s) 187
CviAII CATG 2 cut(s) 362, 434
CviJI RGCY 6 cut(s) 114, 171, 188, 287, 369, 412
CviKI_1 RGCY 6 cut(s) 114, 171, 188, 287, 369, 412
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 16, 256
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 16, 256
EcoT22I ATGCAT 2 cut(s) 391, 435
ErhI CCWWGG 2 cut(s) 16, 256
FaeI CATG 2 cut(s) 365, 437
FaiI YATR 7 cut(s) 26, 296, 339, 352, 363, 431, 435
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 361, 433
Fnu4HI GCNGC 2 cut(s) 97, 474
FokI GGATG 4 cut(s) 205, 215, 395, 454
Fsp4HI GCNGC 2 cut(s) 97, 474
GluI GCNGC 2 cut(s) 97, 474
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 2 cut(s) 114, 171
HgaI GACGC 1 cut(s) 187
Hin1II CATG 2 cut(s) 365, 437
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 3 cut(s) 161, 268, 358
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 5 cut(s) 33, 130, 166, 197, 301
Hpy188III TCNNGA 4 cut(s) 246, 272, 362, 479
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 4 cut(s) 195, 296, 380, 384
HpyCH4III ACNGT 2 cut(s) 61, 317
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 4 cut(s) 99, 389, 433, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 2 cut(s) 365, 437
LmnI GCTCC 2 cut(s) 93, 215
Lsp1109I GCAGC 2 cut(s) 83, 460
LweI GCATC 4 cut(s) 108, 227, 376, 476
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 119, 442
MboII GAAGA 7 cut(s) 48, 59, 62, 65, 274, 277, 464
MlyI GAGTC 2 cut(s) 170, 277
MnlI CCTC 3 cut(s) 160, 178, 191
Mph1103I ATGCAT 2 cut(s) 391, 435
MroXI GAANNNNTTC 1 cut(s) 286
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 473
NlaIII CATG 2 cut(s) 365, 437
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 119
NsiI ATGCAT 2 cut(s) 391, 435
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 93
PctI GAATGC 1 cut(s) 389
PdmI GAANNNNTTC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 2 cut(s) 98, 475
PleI GAGTC 2 cut(s) 169, 276
PpsI GAGTC 2 cut(s) 169, 276
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 3 cut(s) 21, 113, 169
RseI CAYNNNNRTG 1 cut(s) 155
SatI GCNGC 2 cut(s) 97, 474
Sau96I GGNCC 3 cut(s) 21, 113, 169
SchI GAGTC 2 cut(s) 170, 277
SetI ASST 4 cut(s) 81, 87, 126, 289
SfaNI GCATC 4 cut(s) 108, 227, 376, 476
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 155
SsiI CCGC 1 cut(s) 471
StyI CCWWGG 2 cut(s) 16, 256
TaaI ACNGT 2 cut(s) 61, 317
TaiI ACGT 1 cut(s) 126
TfiI GAWTC 1 cut(s) 358
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 96, 473
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 395, 450
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 286
Zsp2I ATGCAT 2 cut(s) 391, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.