RLG00000032482

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010189
Physical Location & Seq
Forward (+)
15735518 .. 15738082
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032482

Sequence Viewer

Length: 498 bp
ATGGAGACGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAGAAGAAGAAGAAACTGTGGATTTGAGCGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAGTACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCCTAAAGACGCAGGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCAAATATATCTGAAGGGAATTTAAACTTTTGGGAGATAAATGCCAAATATAAAAACATTACATATTGGAATCATGACAGCCTTCCTTCGCAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAGCAGCAGAAGATTTGGTTTCTGCATCCGCTGCTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

166

Amino Acids

18.34

Weight (kDa)

5.83

Isoelectric Point (pI)

43.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 28 - 145 1.2e-27 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 75
AciI CCGC 2 cut(s) 75, 474
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 3 cut(s) 142, 294, 493
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 87
Alw44I GTGCAC 1 cut(s) 83
AoxI GGCC 2 cut(s) 115, 172
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 99, 449, 476
ApoI RAATTY 1 cut(s) 310
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 3 cut(s) 21, 116, 172
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 86, 461, 463
BccI CCATC 2 cut(s) 233, 239
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 420
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 3 cut(s) 100, 450, 477
BlsI GCNGC 3 cut(s) 101, 451, 478
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 21, 116, 172
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 4 cut(s) 111, 230, 379, 479
BsaJI CCNNGG 4 cut(s) 16, 168, 199, 259
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse1I ACTGG 2 cut(s) 160, 443
BseDI CCNNGG 4 cut(s) 16, 168, 199, 259
BseGI GGATG 4 cut(s) 221, 231, 391, 470
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 160, 443
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 3 cut(s) 86, 461, 463
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 2 cut(s) 117, 174
BsiHKAI GWGCWC 1 cut(s) 87
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 2 cut(s) 117, 174
Bsp1286I GDGCHC 1 cut(s) 87
BspACI CCGC 2 cut(s) 75, 474
BspANI GGCC 2 cut(s) 117, 174
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 2 cut(s) 160, 443
BssECI CCNNGG 4 cut(s) 16, 168, 199, 259
BssT1I CCWWGG 2 cut(s) 16, 259
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 221, 231, 391, 470
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 3 cut(s) 86, 461, 463
BstXI CCANNNNNNTGG 1 cut(s) 160
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 117, 174
BtsCI GGATG 4 cut(s) 221, 231, 391, 470
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 417
CciI TCATGA 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 21, 116, 172
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 365, 437
CviJI RGCY 6 cut(s) 117, 174, 191, 290, 372, 415
CviKI_1 RGCY 6 cut(s) 117, 174, 191, 290, 372, 415
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 1 cut(s) 32
DraI TTTAAA 1 cut(s) 315
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 16, 259
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 16, 259
EcoT22I ATGCAT 2 cut(s) 394, 438
ErhI CCWWGG 2 cut(s) 16, 259
FaeI CATG 2 cut(s) 368, 440
FaiI YATR 7 cut(s) 26, 299, 342, 355, 366, 434, 438
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 364, 436
Fnu4HI GCNGC 3 cut(s) 100, 450, 477
FokI GGATG 4 cut(s) 208, 218, 398, 457
Fsp4HI GCNGC 3 cut(s) 100, 450, 477
GluI GCNGC 3 cut(s) 100, 450, 477
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 2 cut(s) 117, 174
HgaI GACGC 1 cut(s) 190
Hin1II CATG 2 cut(s) 368, 440
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 3 cut(s) 164, 271, 361
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 5 cut(s) 33, 133, 169, 200, 304
Hpy188III TCNNGA 4 cut(s) 249, 275, 365, 482
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 198, 299, 383, 387
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 5 cut(s) 85, 102, 392, 436, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 368, 440
LmnI GCTCC 2 cut(s) 96, 218
Lsp1109I GCAGC 3 cut(s) 86, 461, 463
LweI GCATC 4 cut(s) 111, 230, 379, 479
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 122
MbiI CCGCTC 1 cut(s) 75
MboII GAAGA 8 cut(s) 48, 59, 62, 65, 68, 277, 280, 467
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 1 cut(s) 310
MlyI GAGTC 2 cut(s) 173, 280
MnlI CCTC 3 cut(s) 163, 181, 194
Mph1103I ATGCAT 2 cut(s) 394, 438
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 392
MwoI GCNNNNNNNGC 1 cut(s) 476
NlaIII CATG 2 cut(s) 368, 440
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 2 cut(s) 394, 438
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 1 cut(s) 96
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 3 cut(s) 101, 451, 478
PleI GAGTC 2 cut(s) 172, 279
PpsI GAGTC 2 cut(s) 172, 279
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 3 cut(s) 21, 116, 172
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 3 cut(s) 100, 450, 477
Sau96I GGNCC 3 cut(s) 21, 116, 172
SchI GAGTC 2 cut(s) 173, 280
SduI GDGCHC 1 cut(s) 87
SetI ASST 4 cut(s) 84, 90, 129, 292
SfaNI GCATC 4 cut(s) 111, 230, 379, 479
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 1 cut(s) 310
SsiI CCGC 2 cut(s) 75, 474
StyI CCWWGG 2 cut(s) 16, 259
TaaI ACNGT 1 cut(s) 64
TaiI ACGT 1 cut(s) 129
TasI AATT 1 cut(s) 310
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 3 cut(s) 99, 449, 476
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 398, 453
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 289
Zsp2I ATGCAT 2 cut(s) 394, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.