RchiOBHm_Chr5g0019891

Ribonuclease H2 subunit C-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010189
Physical Location & Seq
Forward (+)
14287757 .. 14290552
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30002

Sequence Viewer

Length: 489 bp
ATGGACAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAGAAACTGTGGATTTGAGTGGCAAGGTTCACCTGCTCCCCTGCTGCATCAAATACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGGGCCTAAAGACGCAGGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCAAATATATCTGAAGGGAATTCAAACTTTTGGGAGATAAATGCCAAATATAAAAACATTACATATTGGAATCATGACAGCCTTCCTTCGCAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAGCAGCAGAAGATTTGGTTTCTGCATCCGCTGCTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
RNase_H2_suC

Protein Analysis

162

Amino Acids

17.94

Weight (kDa)

7.66

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 25 - 142 4.7e-28 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 87
AciI CCGC 1 cut(s) 465
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 315
AgsI TTSAA 4 cut(s) 133, 285, 306, 484
AloI GAACNNNNNNTCC 2 cut(s) 201, 233
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
AoxI GGCC 2 cut(s) 106, 163
ApeKI GCWGC 3 cut(s) 90, 440, 467
ApoI RAATTY 1 cut(s) 301
Asp700I GAANNNNTTC 1 cut(s) 280
AspS9I GGNCC 3 cut(s) 21, 107, 163
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 21
BaeI ACNNNNGTAYC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 77, 452, 454
BccI CCATC 2 cut(s) 224, 230
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 87
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 3 cut(s) 91, 441, 468
BlsI GCNGC 3 cut(s) 92, 442, 469
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 21, 107, 163
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 4 cut(s) 102, 221, 370, 470
BsaJI CCNNGG 4 cut(s) 16, 159, 190, 250
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bse1I ACTGG 2 cut(s) 151, 434
BseDI CCNNGG 4 cut(s) 16, 159, 190, 250
BseGI GGATG 4 cut(s) 212, 222, 382, 461
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 151, 434
BseXI GCAGC 3 cut(s) 77, 452, 454
BseYI CCCAGC 1 cut(s) 205
BshFI GGCC 2 cut(s) 108, 165
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 108, 165
BspACI CCGC 1 cut(s) 465
BspANI GGCC 2 cut(s) 108, 165
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 151, 434
BssECI CCNNGG 4 cut(s) 16, 159, 190, 250
BssT1I CCWWGG 2 cut(s) 16, 250
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 212, 222, 382, 461
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 77, 452, 454
BstXI CCANNNNNNTGG 1 cut(s) 151
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 2 cut(s) 108, 165
BtsCI GGATG 4 cut(s) 212, 222, 382, 461
BtsIMutI CAGTG 1 cut(s) 144
BveI ACCTGC 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 21, 107, 163
CseI GACGC 1 cut(s) 181
CviAII CATG 2 cut(s) 356, 428
CviJI RGCY 6 cut(s) 108, 165, 182, 281, 363, 406
CviKI_1 RGCY 6 cut(s) 108, 165, 182, 281, 363, 406
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 16, 250
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 16, 250
EcoT22I ATGCAT 2 cut(s) 385, 429
ErhI CCWWGG 2 cut(s) 16, 250
FaeI CATG 2 cut(s) 359, 431
FaiI YATR 7 cut(s) 26, 290, 333, 346, 357, 425, 429
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 355, 427
Fnu4HI GCNGC 3 cut(s) 91, 441, 468
FokI GGATG 4 cut(s) 199, 209, 389, 448
Fsp4HI GCNGC 3 cut(s) 91, 441, 468
GluI GCNGC 3 cut(s) 91, 441, 468
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 2 cut(s) 108, 165
HgaI GACGC 1 cut(s) 181
Hin1II CATG 2 cut(s) 359, 431
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 3 cut(s) 155, 262, 352
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 5 cut(s) 33, 124, 160, 191, 295
Hpy188III TCNNGA 4 cut(s) 240, 266, 356, 473
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 4 cut(s) 189, 290, 374, 378
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 4 cut(s) 93, 383, 427, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 2 cut(s) 359, 431
LmnI GCTCC 2 cut(s) 87, 209
Lsp1109I GCAGC 3 cut(s) 77, 452, 454
LweI GCATC 4 cut(s) 102, 221, 370, 470
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 113
MboII GAAGA 5 cut(s) 48, 59, 268, 271, 458
MluCI AATT 1 cut(s) 301
MlyI GAGTC 2 cut(s) 164, 271
MnlI CCTC 3 cut(s) 154, 172, 185
Mph1103I ATGCAT 2 cut(s) 385, 429
MroXI GAANNNNTTC 1 cut(s) 280
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 1 cut(s) 467
NlaIII CATG 2 cut(s) 359, 431
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 113
NsiI ATGCAT 2 cut(s) 385, 429
PagI TCATGA 1 cut(s) 355
PaqCI CACCTGC 1 cut(s) 87
PctI GAATGC 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 3 cut(s) 92, 442, 469
PleI GAGTC 2 cut(s) 163, 270
PpsI GAGTC 2 cut(s) 163, 270
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 3 cut(s) 21, 107, 163
RseI CAYNNNNRTG 1 cut(s) 149
SatI GCNGC 3 cut(s) 91, 441, 468
Sau96I GGNCC 3 cut(s) 21, 107, 163
SchI GAGTC 2 cut(s) 164, 271
SetI ASST 4 cut(s) 75, 81, 120, 283
SfaNI GCATC 4 cut(s) 102, 221, 370, 470
SfcI CTRYAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 1 cut(s) 301
SsiI CCGC 1 cut(s) 465
StyI CCWWGG 2 cut(s) 16, 250
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 120
TasI AATT 1 cut(s) 301
TfiI GAWTC 1 cut(s) 352
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 3 cut(s) 90, 440, 467
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 2 cut(s) 389, 444
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 301
XmnI GAANNNNTTC 1 cut(s) 280
Zsp2I ATGCAT 2 cut(s) 385, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.